

Reagents for Cloning

Cloning is the process of creating a recombinant DNA molecule, isolating it. and then amplifying and making many more genetically identical copies of the fragment or plasmid. During cloning reactions, it sometimes becomes necessary to purify (clean up) single-stranded or double-stranded DNA obtained from polymerase chain reaction (PCR) amplification. Contaminants may include excess primers, nucleotides, DNA polymerase, mineral oil or salts. One purification method is to bind the DNA to a silica column, wash and elute clean, concentrated DNA in a buffer solution. Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning or microarray analysis.

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NA1020 GenElute PCR Clean-Up Kit sufficient for 70 purifications