CKELR - Performance: Creatine Kinase Isoenzyme Reflex, Serum

Test Catalog

Test ID: CKELR    
Creatine Kinase Isoenzyme Reflex, Serum


Creatine Kinase, Total:



Creatine Kinase Isoenzymes:

Electrophoresis is the migration of charge particles in an electrical field. The rate of migration is dependent on such factors as: 1) net electrical charge of the molecule, 2) size and shape of the molecule, 3) strength of the electrical field, 4) properties of the supporting medium, 5) temperature of the operation.


In this procedure, serum CK is separated into the 3 main isoenzymes of CK (MM, MB, and BB) by electrophoretic separation on agarose gel. After electrophoresis, the separated CK isoenzymes are visualized using a chromogenic substrate. The amount of resulting chromogenic product (formazan precipitate) is proportional to the CK enzymatic activity.


The films are then dried and densitometry is used to quantitate the bands.(Package insert: Sebia Hydragel ISO-CK, Sebia, Norcross, GA)




CK, total: Monday through Sunday; Continuously

CK isoenzymes: Wednesday, Friday; 12 p.m.


1 day-CK Total; 4 days- CK Isoenzymes


2 days-CK Total; 8 days- CK Isoenzymes


1 week

Performing Laboratory Location The location of the laboratory that performs the test