Academics & Faculty

Scientists find vitamin D is crucial in human immune response to tuberculosis

Functions in both innate and adaptive immune reactions, study shows

Vitamin D is not just important for building strong bones — it also plays an essential role in the body's fight against infections such as tuberculosis, an international research team including UCLA scientists has found.
Tuberculosis, a potentially fatal lung disease, causes an estimated 1.8 million deaths annually, according to the World Health Organization, and it especially impacts those with reduced immunity, such as HIV-infected individuals.
In an interesting twist, people with darker skin traditionally have had a higher susceptibility to tuberculosis, and areas of Africa lead the world with the highest infection rates. Scientists believe this may be partly due to the skin pigment melanin. Melanin is more abundant in darker skin, which helps shield the body from ultraviolet rays, but it also reduces vitamin D production.
Vitamin D — a natural hormone, rather than a vitamin — is known to be instrumental in bone development but also may protect against cancer and autoimmune diseases and fight infections.
In a study published online Oct. 12 in the peer-reviewed journal Science Translational Medicine, researchers examined the mechanisms that govern the immune system's ability to kill or inhibit the growth of pathogens such as Myobacterium tuberculosis, the bacteria that causes tuberculosis.
The team found that T cells, which are white blood cells that play a central role in immunity, release a protein called interferon-g that triggers communication between cells and directs infected immune cells to attack the invading tuberculosis bacteria. However, this activation requires sufficient levels of vitamin D to be effective.
Researchers next tested serum taken from blood samples in healthy humans, both with and without sufficient levels of vitamin D. They found that the immune response was not triggered in the serum with lower vitamin D levels, as is found in many African Americans. But, when adequate vitamin D was added to this deficient serum, the immune response was effectively activated.
Scientists found that there was an 85 percent reduction of colony-forming tuberculosis bacteria in human macrophage cells that were effectively treated with interferon-g in the presence of sufficient vitamin D.
"Over the centuries, vitamin D has intrinsically been used to treat tuberculosis. Sanatoriums dedicated to tuberculosis patients were traditionally placed in sunny locations that seemed to help patients — but no one knew why this worked," said the study's first author, Dr. Mario Fabri, who conducted the research at UCLA and is currently a member of the department of dermatology at the University of Cologne in Germany. "Our findings suggest that increasing vitamin D levels through supplementation may improve the immune response to infections such as tuberculosis."
The team noted that vitamin D may help both innate and adaptive immunity, two systems that work synergistically together to fight infections.
Previous research by the team found that vitamin D played a key role in the production of a molecule called cathelicidin, which helps the innate immune system kill the tuberculosis bacteria. Humans are born with innate immunity, which is the preprogrammed part of the immune system.
The current research findings demonstrate that vitamin D is also critical for the action of T cells, key players in adaptive immunity, a highly specialized system that humans acquire over time as they encounter different pathogens.
"The findings of our previous research with innate immunity provided us with a new opportunity to take a look at the effects and role of vitamin D with acquired immunity, both critical systems of human defense," said senior investigator Dr. Robert Modlin, UCLA's Klein Professor of Dermatology and chief of dermatology at the David Geffen School or Medicine at UCLA.
Surprisingly, researchers found that although both the innate and acquired immune systems start out by using different receptors to trigger a complex chain reaction in infected cells to kill the tuberculosis bacteria, both converge early on to follow the same pathway that utilizes vitamin D. 
Specifically, in the current study, researchers discovered that T cells released interferon-g, which not only activated the infected cells called macrophages to generate cathelicidin and other proteins to kill tuberculosis but, like a honing device, also ensured that these proteins were delivered to the compartment of the cell where the bacteria resides. The cells then gobbled up the infectious areas containing bacteria.
"These current findings provide the first credible mechanistic explanation for how vitamin D critically contributes to acquired T cell immunity that protects us from infections, particularly tuberculosis," said Modlin, who also serves as vice chair for cutaneous medicine and dermatological research at UCLA and is a distinguished professor of medicine and microbiology, and of immunology and molecular genetics.
Researchers also noted that this is the first study to demonstrate that the protein interferon-g activates cells to kill the tuberculosis bacteria. 
"The role of interferon-g has been speculated for years in numerous studies, but previous research didn't take into account that sufficient vitamin D was needed to help interferon-g trigger an effective immune response," said study author Dr. John Adams, a professor of orthopaedic surgery at UCLA's Geffen School of Medicine. "Now we understand better how this chain reaction works."
According to the team, the findings are also important because they show that this unique pathway to fight tuberculosis cannot be studied in a mouse model. Mice, as nocturnal animals, are not exposed to the sun enough to absorb vitamin D; as a result, the pathway they use to kill tuberculosis is entirely different from humans.
Fabri noted that most people with tuberculosis are asymptomatic, perhaps due to successful immunological control and sufficient vitamin D to keep the infection from developing into active disease.
"At a time when drug-resistant forms of tuberculosis are emerging, understanding how to enhance natural innate and acquired immunity through vitamin D may be very helpful," said co-author Barry Bloom, former dean of the faculty at the Harvard University School of Public Health, Harvard's Distinguished University Service Professor, and the Jack and Joan Jacobson Professor of Public Health in the School of Public Health's department of immunology and infectious diseases and department of global health and population.
According to researchers, the next step is to initiate clinical trials to learn whether vitamin D supplementation can enhance the body's resistance to tuberculosis and other infections.
The study was funded by the U.S. National Institutes of Health; the Deutsche Forschungsgemeinschaft (German Research Foundation); and the Basic Science Research Program, through the National Research Foundation of Korea.
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