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Measuring leaf or algae blade area in a digital photo with ImageJ

The following is a tutorial on using the freely available ImageJ program from the National Institutes of Health for estimating the area of a leaf or algae blade (or anything else). The process is straightforward once you understand the various steps.

You must begin with a photograph of your object, flattened and dried off as much as practical. Include a clearly visible ruler or other measuring device in the picture. Ideally you will photograph your dark leaf or blade on a light background to maximize the contrast between the object and the background.

[caption id=”attachment_1009″ align=”aligncenter” width=”300″ caption=”The original picture, … Continue Reading