{"action":"created","issue":{"url":"https://api.github.com/repos/openmicroscopy/Imperial-FLIMfit/issues/135","labels_url":"https://api.github.com/repos/openmicroscopy/Imperial-FLIMfit/issues/135/labels{/name}","comments_url":"https://api.github.com/repos/openmicroscopy/Imperial-FLIMfit/issues/135/comments","events_url":"https://api.github.com/repos/openmicroscopy/Imperial-FLIMfit/issues/135/events","html_url":"https://github.com/openmicroscopy/Imperial-FLIMfit/issues/135","id":71065009,"number":135,"title":"Pixel by Pixel fitting on terminalsix prohibitively slow.","user":{"login":"imunro","id":1183110,"avatar_url":"https://avatars.githubusercontent.com/u/1183110?v=3","gravatar_id":"","url":"https://api.github.com/users/imunro","html_url":"https://github.com/imunro","followers_url":"https://api.github.com/users/imunro/followers","following_url":"https://api.github.com/users/imunro/following{/other_user}","gists_url":"https://api.github.com/users/imunro/gists{/gist_id}","starred_url":"https://api.github.com/users/imunro/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/imunro/subscriptions","organizations_url":"https://api.github.com/users/imunro/orgs","repos_url":"https://api.github.com/users/imunro/repos","events_url":"https://api.github.com/users/imunro/events{/privacy}","received_events_url":"https://api.github.com/users/imunro/received_events","type":"User","site_admin":false},"labels":[],"state":"open","locked":false,"assignee":null,"milestone":null,"comments":6,"created_at":"2015-04-26T13:13:05Z","updated_at":"2015-04-27T10:46:33Z","closed_at":null,"body":"As reported by A.Margineanu.  Processing the data following the steps below - is prohibitively slow.\r\nThe progress bar reached 4% after 2 hours before I gave up (running from Source in Matlab) although processing was continuing without errors.\r\n\r\nNB Attempting this on my (Mac) laptop it appeared to reach 36% in around 30 mins before hanging but this has yet to be confirmed using exactly the described steps.\r\n\r\n-\tLoad raw data file: \r\no\t(Fogim_datastore) Z:\\Our Projects\\RASSF plates\\Sectioned plate 2014\\Anca\\sectioned plate all new.raw\r\n-\tLoad ‘Time point calibration file’:\r\no\tZ:\\Our Projects\\RASSF plates\\Sectioned plate 2014\\Anca\\CalibrationData\\fast delay 60MHz CAL80MHz-3.csv\r\n-\tLoad IRF (not ‘Spatially varying IRF’)\r\no\tZ:\\Our Projects\\RASSF plates\\Sectioned plate 2014\\Anca\\CalibrationData\\library irf.xml\r\n-\tLoad ‘Time varying background’:\r\no\tZ:\\Our Projects\\RASSF plates\\Sectioned plate 2014\\Anca\\tvb computed.xml\r\n-\tImport plate metadata:\r\no\tZ:\\Our Projects\\RASSF plates\\Sectioned plate 2014\\Anca\\t0 sectioned last.csv\r\n-\tLoad Acceptor images – Import saved acceptor images:\r\no\tZ:\\Our Projects\\RASSF plates\\Sectioned plate 2014\\Anca\\AcceptorImagesFullPlate.tiff\r\n-\tSegmentation manager – Load segmentation images:\r\no\tZ:\\Our Projects\\RASSF plates\\Sectioned plate 2014\\Anca\\segmentation images no A threshod\r\no\tSelect options – Replace & “ONLY FOV”\r\n-\tImport exclusion list:\r\no\tZ:\\Our Projects\\RASSF plates\\Sectioned plate 2014\\Anca\\bad fov sectioned 800 threshold.txt\r\n-\tSettings: \r\no\tSmoothing 5x5\r\no\tIntegrated min 800\r\no\tTime min 0\r\no\tCounts/Photon 1\r\no\tRep rate 60\r\no\tGate max 2e+04\r\no\tTime max 2e+04\r\no\tBackground – TV Image (960)\r\no\tIRF – Scatter, Background 0, Time min 1300, Time max 4800, IRF shift (-210)\r\no\tAdvanced – Use FOV IRF shift\r\no\tGlobal fitting – Pixel wise\r\no\tNo. Exp 1\r\no\tNo. fixed 0\r\no\tFit contributions – Fitted locally\r\no\tFit IRF shift - Fixed\r\no\tAutomatically estimate lifetimes – checked (Initial tau 2000)\r\n"},"comment":{"url":"https://api.github.com/repos/openmicroscopy/Imperial-FLIMfit/issues/comments/96605982","html_url":"https://github.com/openmicroscopy/Imperial-FLIMfit/issues/135#issuecomment-96605982","issue_url":"https://api.github.com/repos/openmicroscopy/Imperial-FLIMfit/issues/135","id":96605982,"user":{"login":"imunro","id":1183110,"avatar_url":"https://avatars.githubusercontent.com/u/1183110?v=3","gravatar_id":"","url":"https://api.github.com/users/imunro","html_url":"https://github.com/imunro","followers_url":"https://api.github.com/users/imunro/followers","following_url":"https://api.github.com/users/imunro/following{/other_user}","gists_url":"https://api.github.com/users/imunro/gists{/gist_id}","starred_url":"https://api.github.com/users/imunro/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/imunro/subscriptions","organizations_url":"https://api.github.com/users/imunro/orgs","repos_url":"https://api.github.com/users/imunro/repos","events_url":"https://api.github.com/users/imunro/events{/privacy}","received_events_url":"https://api.github.com/users/imunro/received_events","type":"User","site_admin":false},"created_at":"2015-04-27T10:46:33Z","updated_at":"2015-04-27T10:46:33Z","body":"Trying exactly the same sequence of commands on t6 with the installed 4.8.0 build (i.e. MCR) gives the following error when switching Background to to 'TV Image' after loading.  Going to try now from Matlab with the .dll from the build."}}