{"action":"created","issue":{"url":"https://api.github.com/repos/pombase/curation/issues/610","labels_url":"https://api.github.com/repos/pombase/curation/issues/610/labels{/name}","comments_url":"https://api.github.com/repos/pombase/curation/issues/610/comments","events_url":"https://api.github.com/repos/pombase/curation/issues/610/events","html_url":"https://github.com/pombase/curation/issues/610","id":93703367,"number":610,"title":"ofd2 priority paper PMID: 22235339","user":{"login":"pombase-admin","id":13230708,"avatar_url":"https://avatars.githubusercontent.com/u/13230708?v=3","gravatar_id":"","url":"https://api.github.com/users/pombase-admin","html_url":"https://github.com/pombase-admin","followers_url":"https://api.github.com/users/pombase-admin/followers","following_url":"https://api.github.com/users/pombase-admin/following{/other_user}","gists_url":"https://api.github.com/users/pombase-admin/gists{/gist_id}","starred_url":"https://api.github.com/users/pombase-admin/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/pombase-admin/subscriptions","organizations_url":"https://api.github.com/users/pombase-admin/orgs","repos_url":"https://api.github.com/users/pombase-admin/repos","events_url":"https://api.github.com/users/pombase-admin/events{/privacy}","received_events_url":"https://api.github.com/users/pombase-admin/received_events","type":"User","site_admin":false},"labels":[{"url":"https://api.github.com/repos/pombase/curation/labels/auto-migrated","name":"auto-migrated","color":"ededed"},{"url":"https://api.github.com/repos/pombase/curation/labels/high%20priority","name":"high priority","color":"e11d21"},{"url":"https://api.github.com/repos/pombase/curation/labels/sourceforge","name":"sourceforge","color":"ededed"}],"state":"open","locked":false,"assignee":{"login":"mah11","id":5196259,"avatar_url":"https://avatars.githubusercontent.com/u/5196259?v=3","gravatar_id":"","url":"https://api.github.com/users/mah11","html_url":"https://github.com/mah11","followers_url":"https://api.github.com/users/mah11/followers","following_url":"https://api.github.com/users/mah11/following{/other_user}","gists_url":"https://api.github.com/users/mah11/gists{/gist_id}","starred_url":"https://api.github.com/users/mah11/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/mah11/subscriptions","organizations_url":"https://api.github.com/users/mah11/orgs","repos_url":"https://api.github.com/users/mah11/repos","events_url":"https://api.github.com/users/mah11/events{/privacy}","received_events_url":"https://api.github.com/users/mah11/received_events","type":"User","site_admin":false},"milestone":null,"comments":3,"created_at":"2015-05-17T05:49:42Z","updated_at":"2015-09-16T16:47:56Z","closed_at":null,"body":"\r\nhistone H2A dioxygenase\r\nnegative regulation of transcription\r\n\r\nGO:0061428\tnegative regulation of transcription from RNA polymerase II promoter in response to hypoxia\r\n(presumably via chromatin remodelling?)\r\n\r\nI am adding an NAS to \r\nGO:0061428\tnegative regulation of transcription from RNA polymerase II promoter in response to hypoxia to  get this grouped for my figure....\r\n\r\n\n\nOriginal comment by: ValWood"},"comment":{"url":"https://api.github.com/repos/pombase/curation/issues/comments/140799584","html_url":"https://github.com/pombase/curation/issues/610#issuecomment-140799584","issue_url":"https://api.github.com/repos/pombase/curation/issues/610","id":140799584,"user":{"login":"mah11","id":5196259,"avatar_url":"https://avatars.githubusercontent.com/u/5196259?v=3","gravatar_id":"","url":"https://api.github.com/users/mah11","html_url":"https://github.com/mah11","followers_url":"https://api.github.com/users/mah11/followers","following_url":"https://api.github.com/users/mah11/following{/other_user}","gists_url":"https://api.github.com/users/mah11/gists{/gist_id}","starred_url":"https://api.github.com/users/mah11/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/mah11/subscriptions","organizations_url":"https://api.github.com/users/mah11/orgs","repos_url":"https://api.github.com/users/mah11/repos","events_url":"https://api.github.com/users/mah11/events{/privacy}","received_events_url":"https://api.github.com/users/mah11/received_events","type":"User","site_admin":false},"created_at":"2015-09-16T16:47:56Z","updated_at":"2015-09-16T16:47:56Z","body":"In progress. Two questions:\r\n\r\n1. I'm a bit confused now about whether to use GO:0061428 for ofd2. In the deletion mutant, genes that are up-regulated in hypoxia behave the same as in wt, but a subset repressed genes seem to be more repressed. So would you still infer a role in \"negative regulation of transcription ...\" from that effect?\r\n2. Would you annotate anything for the experiment in Figure 2C, where they made some peptides corresponding to a bit of H2A that seems to be important for whatever Ofd2 is doing to modify the histone? It could be captured as phenotypes of mutations in hta1/hta2, if we believe that the effect would be roughly the same with the whole pombe proteins. Can't think of any other sensible way to do it.\r\n"}}