{"action":"created","issue":{"url":"https://api.github.com/repos/uchicago-bio/2015-Autumn-Forum/issues/38","labels_url":"https://api.github.com/repos/uchicago-bio/2015-Autumn-Forum/issues/38/labels{/name}","comments_url":"https://api.github.com/repos/uchicago-bio/2015-Autumn-Forum/issues/38/comments","events_url":"https://api.github.com/repos/uchicago-bio/2015-Autumn-Forum/issues/38/events","html_url":"https://github.com/uchicago-bio/2015-Autumn-Forum/issues/38","id":112940744,"number":38,"title":"BlastN search: E-values are all zero?","user":{"login":"bjmc","id":328557,"avatar_url":"https://avatars.githubusercontent.com/u/328557?v=3","gravatar_id":"","url":"https://api.github.com/users/bjmc","html_url":"https://github.com/bjmc","followers_url":"https://api.github.com/users/bjmc/followers","following_url":"https://api.github.com/users/bjmc/following{/other_user}","gists_url":"https://api.github.com/users/bjmc/gists{/gist_id}","starred_url":"https://api.github.com/users/bjmc/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/bjmc/subscriptions","organizations_url":"https://api.github.com/users/bjmc/orgs","repos_url":"https://api.github.com/users/bjmc/repos","events_url":"https://api.github.com/users/bjmc/events{/privacy}","received_events_url":"https://api.github.com/users/bjmc/received_events","type":"User","site_admin":false},"labels":[],"state":"open","locked":false,"assignee":null,"milestone":null,"comments":1,"created_at":"2015-10-23T03:25:18Z","updated_at":"2015-10-24T12:42:58Z","closed_at":null,"body":"On question 1, when I ran the BlastN search, my results summary shows every sequence with an E-value of 0.0. Is that correct? Did I do something wrong? The only change I made from the default parameters was to switch the search program from \"megablast\" to \"blastn\"\r\n\r\n![screenshot from 2015-10-22 22 23 01](https://cloud.githubusercontent.com/assets/328557/10683811/935e2488-790b-11e5-9c8a-fb660a913b03.png)\r\n"},"comment":{"url":"https://api.github.com/repos/uchicago-bio/2015-Autumn-Forum/issues/comments/150807783","html_url":"https://github.com/uchicago-bio/2015-Autumn-Forum/issues/38#issuecomment-150807783","issue_url":"https://api.github.com/repos/uchicago-bio/2015-Autumn-Forum/issues/38","id":150807783,"user":{"login":"tabinks","id":93689,"avatar_url":"https://avatars.githubusercontent.com/u/93689?v=3","gravatar_id":"","url":"https://api.github.com/users/tabinks","html_url":"https://github.com/tabinks","followers_url":"https://api.github.com/users/tabinks/followers","following_url":"https://api.github.com/users/tabinks/following{/other_user}","gists_url":"https://api.github.com/users/tabinks/gists{/gist_id}","starred_url":"https://api.github.com/users/tabinks/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/tabinks/subscriptions","organizations_url":"https://api.github.com/users/tabinks/orgs","repos_url":"https://api.github.com/users/tabinks/repos","events_url":"https://api.github.com/users/tabinks/events{/privacy}","received_events_url":"https://api.github.com/users/tabinks/received_events","type":"User","site_admin":false},"created_at":"2015-10-24T12:42:58Z","updated_at":"2015-10-24T12:42:58Z","body":"Something is fishy there.  Make sure you are searching protein sequence \"NP_\" against blastp."}}