{"action":"created","issue":{"url":"https://api.github.com/repos/veg/hyphy/issues/279","labels_url":"https://api.github.com/repos/veg/hyphy/issues/279/labels{/name}","comments_url":"https://api.github.com/repos/veg/hyphy/issues/279/comments","events_url":"https://api.github.com/repos/veg/hyphy/issues/279/events","html_url":"https://github.com/veg/hyphy/issues/279","id":69693792,"number":279,"title":"The same gene, but multiple insertion/deletions...","user":{"login":"AndrzejN","id":12040907,"avatar_url":"https://avatars.githubusercontent.com/u/12040907?v=3","gravatar_id":"","url":"https://api.github.com/users/AndrzejN","html_url":"https://github.com/AndrzejN","followers_url":"https://api.github.com/users/AndrzejN/followers","following_url":"https://api.github.com/users/AndrzejN/following{/other_user}","gists_url":"https://api.github.com/users/AndrzejN/gists{/gist_id}","starred_url":"https://api.github.com/users/AndrzejN/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/AndrzejN/subscriptions","organizations_url":"https://api.github.com/users/AndrzejN/orgs","repos_url":"https://api.github.com/users/AndrzejN/repos","events_url":"https://api.github.com/users/AndrzejN/events{/privacy}","received_events_url":"https://api.github.com/users/AndrzejN/received_events","type":"User","site_admin":false},"labels":[{"url":"https://api.github.com/repos/veg/hyphy/labels/Web","name":"Web","color":"02d7e1"}],"state":"open","locked":false,"assignee":{"login":"spond","id":1018513,"avatar_url":"https://avatars.githubusercontent.com/u/1018513?v=3","gravatar_id":"","url":"https://api.github.com/users/spond","html_url":"https://github.com/spond","followers_url":"https://api.github.com/users/spond/followers","following_url":"https://api.github.com/users/spond/following{/other_user}","gists_url":"https://api.github.com/users/spond/gists{/gist_id}","starred_url":"https://api.github.com/users/spond/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/spond/subscriptions","organizations_url":"https://api.github.com/users/spond/orgs","repos_url":"https://api.github.com/users/spond/repos","events_url":"https://api.github.com/users/spond/events{/privacy}","received_events_url":"https://api.github.com/users/spond/received_events","type":"User","site_admin":false},"milestone":null,"comments":2,"created_at":"2015-04-20T22:18:28Z","updated_at":"2015-04-20T22:55:58Z","closed_at":null,"body":"Hi! Here is my VERY basic question. I'm trying to detect regions of gene under positive selection. I have a set of gene coding sequences, however this sequences have MANY deletions/insertions and different STOP codons. \r\n\r\nI'm unable to upload this sequences to DataMonkey.\r\n\r\nI edit sequences (start/stop codon), did alignment using Muscle, then reverted it back to nucleotide sequence, whole alignment was saved in nexus format. DataMonkey is rejecting my sequences. I'm getting to the point to see gene names with indication at what position are STOP codons in my sequences, and that they are different.\r\n\r\nWhy I can't upload this sequences?\r\n\r\nANY help would be greatly appreciated.\r\n\r\nBTW. I can run HyPhy analysis using mentioned alignment in MEGA 6... "},"comment":{"url":"https://api.github.com/repos/veg/hyphy/issues/comments/94587258","html_url":"https://github.com/veg/hyphy/issues/279#issuecomment-94587258","issue_url":"https://api.github.com/repos/veg/hyphy/issues/279","id":94587258,"user":{"login":"AndrzejN","id":12040907,"avatar_url":"https://avatars.githubusercontent.com/u/12040907?v=3","gravatar_id":"","url":"https://api.github.com/users/AndrzejN","html_url":"https://github.com/AndrzejN","followers_url":"https://api.github.com/users/AndrzejN/followers","following_url":"https://api.github.com/users/AndrzejN/following{/other_user}","gists_url":"https://api.github.com/users/AndrzejN/gists{/gist_id}","starred_url":"https://api.github.com/users/AndrzejN/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/AndrzejN/subscriptions","organizations_url":"https://api.github.com/users/AndrzejN/orgs","repos_url":"https://api.github.com/users/AndrzejN/repos","events_url":"https://api.github.com/users/AndrzejN/events{/privacy}","received_events_url":"https://api.github.com/users/AndrzejN/received_events","type":"User","site_admin":false},"created_at":"2015-04-20T22:55:58Z","updated_at":"2015-04-20T22:55:58Z","body":"Yes, my sequences have TAA and TGA codons, but why DataMonkey would reject this sequences?\r\n\r\nAndrzej\r\n\r\nPs. I would rather not sent you my data :)."}}