{"action":"opened","issue":{"url":"https://api.github.com/repos/TGAC/bioruby-polyploid-tools/issues/5","labels_url":"https://api.github.com/repos/TGAC/bioruby-polyploid-tools/issues/5/labels{/name}","comments_url":"https://api.github.com/repos/TGAC/bioruby-polyploid-tools/issues/5/comments","events_url":"https://api.github.com/repos/TGAC/bioruby-polyploid-tools/issues/5/events","html_url":"https://github.com/TGAC/bioruby-polyploid-tools/issues/5","id":75950728,"number":5,"title":"Primer3 preference File","user":{"login":"thomasmaggen","id":12427820,"avatar_url":"https://avatars.githubusercontent.com/u/12427820?v=3","gravatar_id":"","url":"https://api.github.com/users/thomasmaggen","html_url":"https://github.com/thomasmaggen","followers_url":"https://api.github.com/users/thomasmaggen/followers","following_url":"https://api.github.com/users/thomasmaggen/following{/other_user}","gists_url":"https://api.github.com/users/thomasmaggen/gists{/gist_id}","starred_url":"https://api.github.com/users/thomasmaggen/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/thomasmaggen/subscriptions","organizations_url":"https://api.github.com/users/thomasmaggen/orgs","repos_url":"https://api.github.com/users/thomasmaggen/repos","events_url":"https://api.github.com/users/thomasmaggen/events{/privacy}","received_events_url":"https://api.github.com/users/thomasmaggen/received_events","type":"User","site_admin":false},"labels":[],"state":"open","locked":false,"assignee":null,"milestone":null,"comments":0,"created_at":"2015-05-13T11:23:34Z","updated_at":"2015-05-13T11:23:34Z","closed_at":null,"body":"Hi,\r\nThis is really a useful tool! However, I sometimes encounter the problem that primers could not be designed because no primers meet the criteria set by primer3. I notice there is an option to upload a primer3preference file, so i want to try this to use more relaxed parameters.... How should the preference file be formatted?\r\nMany thanks,\r\nThomas."}}