No
During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, antiparallel RNA strand called a primary transcript. Transcription proceeds in the following general steps: 1  One or more sigma factor protein binds to the RNA polymerase holoenzyme, allowing it to bind to promoter DNA. The antisense strand of DNA is read by RNA polymerase from the 3' end to the 5' end during transcription (3' → 5'). The complementary RNA is created in the opposite direction, in the 5' → 3' direction, matching the sequence of the sense strand with the exception of switching uracil for thymine. Unlike DNA polymerase, RNA polymerase doesn't require a primer-it can synthesize RNA (in the form of mRNA) de novo. RNA polymerase also unwinds the DNA double helix itself to make it available for transcription. 4 years ago. The enzyme, RNA polymerase and DNA polymerase are different (yet they nearly do the same thing) - this doesn't mean they are identical. RNA polymerase does not require the 3' OH to initiate synthesis of RNA molecules. It's as simple as that. During initiation of DNA replication, DNA nucleotide triphosphates must be attached to a 3' OH of an RNA molecule by DNA polymerase. This process, in itself, removes the terminal two phosphates of the nucleotides In bacteria, transcription begins with the binding of RNA polymerase to the promoter in DNA. RNA polymerase is a core enzyme consisting of five subunits: 2 α subunits, 1 β subunit, 1 β' subunit, and 1 ω subunit. The antisense strand of DNA is read by RNA polymerase from the 3' end to the 5' end during transcription (3' → 5'). The complementary RNA is created in the opposite direction, in the 5' → 3' direction, matching the sequence of the sense strand with the exception of switching uracil for thymine. In eukaryotes, RNA polymerase, and therefore the initiation of transcription, requires the presence of a core promoter sequence in the DNA. Promoters are regions of DNA that promote transcription and, in eukaryotes, are found at -30, -75, and -90 base pairs upstream from the start site of transcription. RNA is transcribed in the nucleus; processed, it is transported to the cytoplasm and translated by the ribosome. At the end of its life, the mRNA is degraded. In transcription, the codons of a gene are copied into messenger RNA by RNA polymerase.In simple word or formation of RNA from DNA is known as DNA polymerase requires a primer because it can't initiate polymerization by it self only,but requires a preexisting free 3'OH group to which it can add deoxynucleotides formi … ng phosphodiester bond & the free 3'OH group is provided by the primer.Therefore the DNA polymerase requires primer.  Transcription is the process by which DNA is copied (transcribed) to mRNA, which carries the information needed for protein synthesis. Transcription takes place in two broad steps. First, pre-messenger RNA is formed, with the involvement of RNA polymerase enzymes.  1 One or more sigma factor protein binds to the RNA polymerase holoenzyme, allowing it to bind to promoter DNA. 2  RNA polymerase creates a transcription bubble, which separates the two strands of the DNA helix. 3  This is done by breaking the hydrogen bonds between complementary DNA nucleotides. The antisense strand of DNA is read by RNA polymerase from the 3' end to the 5' end during transcription (3' → 5'). The complementary RNA is created in the opposite direction, in the 5' → 3' direction, matching the sequence of the sense strand with the exception of switching uracil for thymine.
https://en.wikipedia.org/wiki/Transcription_(genetics) http://biology-forums.com/index.php?topic=3415.0 https://en.wikipedia.org/wiki/Transcription_(genetics) https://en.wikibooks.org/wiki/An_Introduction_to_Molecular_Biology/Transcription_of_RNA_and_its_modification http://www.answers.com/Q/Is_DNA_polymerase_required_for_transcription http://www.atdbio.com/content/14/Transcription-Translation-and-Replication https://en.wikipedia.org/wiki/Transcription_(genetics)
does transcription require dna polymerase