PH, which is a measure of hydrogen ion concentration, will affect the enzyme by changing the concentration of hydrogen in that enzyme.
Why enzyme activity is affected by pH. by Bhav Patel · October 10, 2014. Why enzyme activity is affected by pH. A pH is the measure of the concentration of hydrogen ions, H+ with their values ranging from 1 -14. pH 7 is said to be neutral, any value below this is said to be acidic and values above are alkaline. By doing this, it interferes with the binding of the substrate to the active site and it therefore changes the rate of activity of the enzyme – the rate of an enzyme controlled reaction). Every enzyme has their own optimum pHs; the pH where the rate of the enzyme controlled reaction is at its highest. PH changes affect the structure of an enzyme molecule and therefore. affect its ability to bind with its substrate molecules. Changes in pH. affect the ionic bonds and hydrogen bonds that hold the enzyme. together, which naturally affects the rate of reaction of the enzyme. with the substrate. The optimum pH for most enzymes is pH7. In the body, amylase works mainly in the small intestines, where the. acidity from the stomach has been neutralised by the hydrogencarbonate. ions in the pancreatic juices secreted by the pancreas. Effects of pH. Enzymes are affected by changes in pH. The most favorable pH value-the point where the enzyme is most active-is known as the optimum pH. Extremely high or low pH values generally result in complete loss of activity for most enzymes. pH is also a factor in the stability of enzymes. As with activity, for each enzyme there is also a region of pH optimal stability. In addition to temperature and pH there are other factors, such as ionic strength, which can affect the enzymatic reaction. Effects of pH. Enzymes are affected by changes in pH. The most favorable pH value-the point where the enzyme is most active-is known as the optimum pH. This is graphically illustrated in Figure 14. Extremely high or low pH values generally result in complete loss of activity for most enzymes This was received at pH 2. The pH has an affect on enzymes as the three-dimensional shape that enzymes molecular structure is based on, is partly the result of hydrogen bonding. PH, which is a measure of hydrogen ion concentration, will affect the enzyme by changing the concentration of hydrogen in that enzyme. Any change in pH can effectively denature enzymes. My anomalous results were identified and a justification for the reason behind it was explained. Other variable like temperature, enzyme concentration, substrate concentration, the affect of competitive and non-competitive inhibitors are all possibilities on the affect of enzymes. Concluding my investigating, my results support my hypothesis. At a pH level either too high or too low level would denature the enzyme. Amylase is an enzyme that breaks starch (a complex carbohydrate) into its simple sugar, glucose. Starch is also known as amylose.   Enzyme activity is sensitive t … o the temperature and pH of its environment. At the wrong temperature or pH, the enzyme's structure will degrade. pH is also a factor in the stability of enzymes. As with activity, for each enzyme there is also a region of pH optimal stability. In addition to temperature and pH there are other factors, such as ionic strength, which can affect the enzymatic reaction. Changes in pH may not only affect the shape of an enzyme but it may also change the shape or charge properties of the substrate so that either the substrate connot bind to the active site or it cannot undergo catalysis. The pH of a solution can have several effects of the structure and activity of enzymes. For example, pH can have an effect of the state of ionization of acidic or basic amino acids. Acidic amino acids have carboxyl functional groups in their side chains. otherwise affect the reaction of starch with amylase. 1. Temperature: As the temperature is increased, the rate at which. enzyme-substrate complexes are formed also increases, due to the. increase in kinetic energy, increasing the rate of reaction. The optimum pH for most enzymes is pH7. In the body, amylase works mainly in the small intestines, where the. acidity from the stomach has been neutralised by the hydrogencarbonate. ions in the pancreatic juices secreted by the pancreas. Above or below an enzyme’s optimum pH, its activity is lower. The optimum pH of a particular enzyme corresponds to the pH of its natural environment. For many enzymes, this corresponds to pH values of around 7. For pepsin, which is active in the stomach, the optimum pH is 2 (the pH of the stomach).  An amylase (/ˈaemɨleɪs/) ˈæmɨleɪs is an enzyme that catalyses the hydrolysis of starch into. Sugars amylase is present in the saliva of humans and some other, mammals where it begins the chemical process of. digestion 
http://www.sciencenutshell.com/enzyme-activity-affected-ph/ http://www.123helpme.com/view.asp?id=116243 http://www.answers.com/Q/How_does_pH_affect_amylase_activity http://www.worthington-biochem.com/introbiochem/effectsph.html http://www.markedbyteachers.com/as-and-a-level/science/how-does-ph-affect-the-denaturation-of-enzymes-starch-and-amylase.html http://www.answers.com/Q/How_does_pH_affect_amylase_activity http://academic.brooklyn.cuny.edu/biology/bio4fv/page/ph_and_.htm http://www.123helpme.com/view.asp?id=116243 http://www.laney.edu/wp/cheli-fossum/files/2012/01/10-Enzymes.pdf https://en.wikipedia.org/wiki/Amylase
why is enzyme amylase affected by ph