This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator. It triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator.
Isopropyl β-D-1-thiogalactopyranoside (IPTG) is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator. For induction, a sterile, filtered 1 M solution of IPTG is typically added by 1:1000 dilution into a logarithmically growing bacterial culture, however, different final concentration of IPTG may also be used. Protein expression with IPTG -. Hi, I use a pET vector system where the expression of my protein of interest is regulated by the lac operon. I want to express my protein in transformated E.coli in 100 ml of LB broth, so I need to add IPTG to the broth.  Hey guys, I was wondering of someone could tell me, how does IPTG induce the protein expression. I am trying to express the Scrapie Prion protein PrP in E coli. I know that the IPTG is an artificial inducer, however, I understand that it would induce the production of enzyme to metabolise IPTG but how does it start the expression of my protein of interest. Thanks in adavance for your answers. sta IPTG binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner, thereby allowing the transcription of genes in the lac operon, such as the gene coding for beta-galactosidase, a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. One advantage of IPTG for in vivo studies is that since it cannot be metabolized by E. coli its concentration remains constant and the rate of expression of lac p/o-controlled genes, is not a variable in the experiment. Thermo Scientific IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon. It is used in conjunction with X-Gal (#R0941) to determine the lac phenotype in blue/white colony screening. Applications. • Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies IPTG is a molecular biology reagent utilized in cloning experiments. This compound is used as a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon. One advantage of IPTG for in vivo studies is that since it cannot be metabolized by E. coli its concentration remains constant and the rate of expression of lac p/o-controlled genes, IPTG is not a variable in the experiment. lac operon (lactose operon) is an operon required for the transport and metabolism of lactose in Escherichia coli and many other enteric bacteria. Although glucose is the preferred carbon source for most bacteria, the lac operon allows for the effective digestion of lactose when glucose is not available. In this case, when lactose is required as a sugar source for the bacterium, the three genes of the lac operon can be expressed and their subsequent proteins translated: lacZ, lacY, and lacA. Background. IPTG (isopropyl beta-D-thiogalactoside) Inducer for Beta-Galactosidase Expression acts as a molecular mimic of a lactose metabolite. The presence of IPTG triggers the activation of the lac operon for downstream gene transcription due to its binding the lac repressor. Due to the presence of a sulfur atom in IPTG, the cells cannot degrade the inducer and therefore its concentration remains constant. IPTG (isopropyl beta-D-thiogalactoside) Inducer for Beta-Galactosidase Expression is ideal for investigators in Immunology, Cell Biology, and Microbiology research
https://en.wikipedia.org/wiki/IPTG http://www.protocol-online.org/biology-forums/posts/27243.html http://www.protocol-online.org/biology-forums/posts/10899.html http://agscientific.com/blog/index.php/2013/01/08/iptg-triggers-the-transcription-of-the-lac-operon/ https://www.thermofisher.com/order/catalog/product/R1171 http://agscientific.com/blog/index.php/2012/08/17/14-need-to-know-iptg-faqs-and-protocols/ https://en.wikipedia.org/wiki/Lac_operon http://www.rockland-inc.com/store/Enzyme-Substrates-IPTG-1000-O4L_12120.aspx
what does iptg do