To ensure the proteins in the cell aren't separated from the rest of the solution with the DNA.
C. Performing the DNA Extraction: 1  Add 1 milliliter of the Extraction Solution/Buffer A (salt solution) to the large test tube. 2  Recap the tube and mix gently for 10 seconds. 3  Add 1 milliliter of the Extraction Solution/Buffer B (soap solution) to the test tube. 1 Extracting DNA involves a series of steps to gently break open the cell, break open the nuclear membrane, separate the DNA from proteins and then cause it to precipitate out of a solution. This is accomplished using various chemicals, based on the structure of membranes, DNA and its electronegativity. Sodium chloride, or other sodium-containing compounds, are used to stabilize the DNA after it has been stripped of its proteins and aid in precipitation. To recover DNA for further testing or visualization, the DNA must be precipitated out of a solution with water. Since water has a relatively weak positive charge, this is accomplished by providing a stronger positively charged ion in the solution. Sodium is the perfect candidate for this. Several different forms of sodium are used in DNA extraction. Sodium dodecyl sulfate, or SDS. is a sodium-containing detergent. It has the chemical formula of C12H25NaO4S, where the Na stands for sodium. Detergents are used to break down cell walls and membranes. Sodium hydroxide is a base. A solution of sodium hydroxide makes the solution very basic or alkaline. Sodium hydroxide can act by loosening the rigid structure of a cell wall or membrane, thereby releasing the DNA. Sodium hydroxide is most often used in plasmid DNA extraction. Sodium chloride was needed to ensure the proteins in the cell aren't separated from the rest of the solution with the DNA. Okay, the person above does not understand Chemistry nor the English language. The sodium chloride, NaCl, is a polar molecule, which would help separate an emulsion of two layers in an acid-base extraction. Since NaCl is polar, it would be easily dissolved into the solution and tie up the different layer molecules. Cellular and histone proteins bound to the DNA can be removed either by adding a protease or by having precipitated the proteins with sodium or ammonium acetate, or extracted … them with a phenol-chloroform mixture prior to the DNA-precipitation. Okay, the person above does not understand Chemistry nor the English language. The sodium chloride, NaCl, is a polar molecule, which would help separate an emulsion of two layers in an acid-base extraction. Since NaCl is polar, it would be easily dissolved into the solution and tie up the different layer molecules. Sodium Acetate's Role. Sodium can also be in the form of sodium acetate. Like sodium hydroxide, sodium acetate is used to help separate plasmid DNA from chromosomal DNA, but by a much different mechanism and at a different part of the DNA extraction procedure. Single strands of linear DNA are insoluble in high salt. Sodium hydroxide is a base. A solution of sodium hydroxide makes the solution very basic or alkaline. Sodium hydroxide can act by loosening the rigid structure of a cell wall or membrane, thereby releasing the DNA. Sodium hydroxide is most often used in plasmid DNA extraction. Answered by The Community. Making the world better, one answer at a time. Brine (saturated sodium chloride solution) is usually the last solution used in an aqueous wash to help remove trace amounts of water (and anything water soluble) from the organic layer. The sodium chloride, NaCl, is a polar molecule, which would help separate an emulsion of two layers in an acid-base extraction. Since NaCl is polar, it would be easily dissolved into the solution and tie up the different layer molecules. Brine (saturated sodium chloride solution) is usually the last solution used in an aqueous wash to help remove trace amounts of water (and anything water soluble) from t … he organic layer. Okay, the person above does not understand Chemistry nor the English language. The sodium chloride, NaCl, is a polar molecule, which would help separate an emulsion of two layers in an acid-base extraction. Since NaCl is polar, it would be easily dissolved into the solution and tie up the different layer molecules.
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what is the role of the sodium chloride detergent solution in the dna extraction process