Technology in molecular biology used to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence
Definition of POLYMERASE CHAIN REACTION. : an in vitro technique for rapidly synthesizing large quantities of a given DNA segment that involves separating the DNA into its two complementary strands, using DNA polymerase to synthesize two-stranded DNA from each single strand, and repeating the process —abbreviation PCR. ADVERTISEMENT. polymerase chain reaction / PCR. Polymerase chain reaction, or PCR, is a laboratory technique used to make multiple copies of a segment of DNA. PCR is very precise and can be used to amplify, or copy, a specific DNA target from a mixture of DNA molecules. Then, to perform PCR, the DNA template that contains the target is added to a tube that contains primers, free nucleotides, and an enzyme called DNA polymerase, and the mixture is placed in a PCR machine. The PCR machine increases and decreases the temperature of the sample in automatic, programmed steps. Freebase (0.00 / 0 votes) Rate this definition: Polymerase chain reaction. The polymerase chain reaction is a biochemical technology in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.  PCR (polymerase chain reaction): PCR (polymerase chain reaction) is a technique in molecular genetics that permits the analysis of any short sequence of DNA (or RNA) even in samples containing only minute quantities of DNA or RNA. PCR is used to reproduce (amplify) selected sections of DNA or RNA for analysis.  Reverse transcription polymerase chain reaction (RT-PCR) is one of many variants of polymerase chain reaction (PCR) . This technique is commonly used in molecular biology to detect RNA expression. RT-PCR is often confused with real-time polymerase chain reaction (qPCR) by students and scientists alike.  Polymerase chain reaction (PCR) is a way to make many copies of a sequence of DNA. This is done in a lab, using an enzyme called DNA polymerase. It is called chain reaction because the result of one cycle is used immediately for the next cycle. This allows exponential growth to happen.  PCR (polymerase chain reaction) is a method to analyze a short sequence of DNA (or RNA) even in samples containing only minute quantities of DNA or RNA. PCR is used to reproduce (amplify) selected sections of DNA or RNA.  First, two short DNA sequences called primers are designed to bind to the start and end of the DNA target. Then, to perform PCR, the DNA template that contains the target is added to a tube that contains primers, free nucleotides, and an enzyme called DNA polymerase, and the mixture is placed in a PCR machine. The PCR machine increases and decreases the temperature of the sample in automatic, programmed steps. The polymerase chain reaction (PCR) is a technology in molecular biology used to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. 1 In silico PCR (digital PCR, virtual PCR, electronic PCR, e-PCR) refers to computational tools used to calculate theoretical polymerase chain reaction results using a given set of primers (probes) to amplify DNA sequences from a sequenced genome or transcriptome.
http://www.merriam-webster.com/dictionary/polymerase%20chain%20reaction http://www.nature.com/scitable/definition/polymerase-chain-reaction-PCR-110 http://www.definitions.net/definition/polymerase%20chain%20reaction http://www.medicinenet.com/script/main/art.asp?articlekey=4807 https://en.wikipedia.org/wiki/Reverse_transcription_polymerase_chain_reaction https://simple.wikipedia.org/wiki/Polymerase_chain_reaction http://www.medicinenet.com/pcr_polymerase_chain_reaction/article.htm http://www.nature.com/scitable/definition/polymerase-chain-reaction-PCR-110 https://en.wikipedia.org/wiki/Polymerase_chain_reaction
polymerase chain reaction definition