DNA–DNA hybridization generally refers to a molecular biology technique that measures the degree of genetic similarity between pools of DNA sequences. It is usually used to determine the genetic distance between two organisms.This has been used a lot in phylogeny and taxonomy.
Scientists studying the chemistry of DNA moved even closer to actual sequences. Charles Sibley (left) and Jon Ahlquist pioneered the use of DNA kinetics to investigate evolutionary relationships using a technique called DNA-DNA hybridization (see figure, right). Each DNA molecule is made of two strands of nucleotides.  Whole genomic DNA-DNA hybridization has been a cornerstone of bacterial species determination but is not widely used because it is not easily implemented. We have developed a method based on random genome fragments and DNA microarray technology that overcomes the disadvantages of whole-genome DNA-DNA hybridization. DNA-DNA hybridization is one method that provides more resolution than 16S rDNA sequencing, and the 70% criterion (22) has been a cornerstone for describing a bacterial species. Hybridization is the process of combining two complementary single-stranded DNA or RNA molecules and allowing them to form a single double-stranded molecule through base pairing.  DNA–DNA hybridization is the gold standard to distinguish bacterial species, with a similarity value smaller than 70% indicating that the compared strains belong to distinct species. In 2014, a threshold of 79% similarity has been suggested to separate bacterial subspecies. The DNA of one organism is labeled, then mixed with the unlabeled DNA to be compared against. The mixture is incubated to allow DNA strands to dissociate and renewal forming hybrid double-stranded DNA. Search for: Biology Glossary search by EverythingBio.com. DNA-RNA hybridization When a mixture of DNA and RNA is heated to denaturation temperatures to form single strands and then cooled RNA can hybridize (form a double helix) with DNA that has a complementary nucleotide sequence.  DNA-DNA hybridization When DNA is heated to denaturation temperatures to form single strands and then cooled double helices will re-form (renaturation) at regions of sequence complementarity.  DNA–DNA hybridization generally refers to a molecular biology technique that measures the degree of genetic similarity between pools of DNA sequences. It is usually used to determine the genetic distance between two organisms. This has been used a lot in phylogeny and taxonomy. The DNA of one organism is labeled, then mixed with the unlabeled DNA to be compared against. The mixture is incubated to allow DNA strands to dissociate and renewal forming hybrid double-stranded DNA. Please try again later. A very simplistic explanation of DNA hybridization is when a single-stranded DNA (ssDNA) molecule bonds with a complementary ssDNA molecule from another source forming a hybrid. This animation shows a double-stranded DNA (dsDNA) molecule dividing into two ssDNA strands.  DNA-DNA hybridization, the formation of double-helical DNA from two complementary single strands. It is used to compare genome relationships between different species. 1  Facebook.  © A Dictionary of Zoology 1999, originally published by Oxford University Press 1999. DNA-DNA hybridization A technique that is used to compare DNA from two different species, to locate or identify nucleotide sequences, and to establish the effective in vitro transfer of nuclear material to a new host. Evolution deals with heritable changes in populations over time. Because DNA is the molecule of heredity, evolutionary changes will be reflected in changes in the base pairs in DNA. Two species that have evolved from a common ancestor will have DNA that has very similar base pair sequences.
http://www.evolution.berkeley.edu/evosite/history/genetsims2.shtml http://aem.asm.org/content/67/8/3677.full http://www.ghr.nlm.nih.gov/glossary=dnahybridization https://en.wikipedia.org/wiki/DNA-DNA_Hybridization http://groups.molbiosci.northwestern.edu/holmgren/Glossary/Definitions/Def-D/DNA-RNA_hybridization.html http://groups.molbiosci.northwestern.edu/holmgren/Glossary/Definitions/Def-D/DNA-DNA_hybridization.html https://en.wikipedia.org/wiki/DNA-DNA_Hybridization http://www.youtube.com/watch?v=0qoqzErrae4 http://medical-dictionary.thefreedictionary.com/DNA-DNA+hybridization http://www.encyclopedia.com/topic/DNA_Hybridization.aspx
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