It is a test that detects and measures antibodies in your blood.This test can be used to determine if you have antibodies that are related to certain infectious conditions.Antibodies are proteins that the body produces in response to harmful substances (antigens).
Best Answer: The Enzyme-Linked ImmunoSorbent Assay, or (ELISA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. It uses two antibodies. One antibody is specific to the antigen. The other reacts to antigen-antibody complexes, and is coupled to an enzyme. This second antibody, which accounts for enzyme-linked in the test's name, can also cause a chromogenic or fluorogenic substrate to produce a signal. ELISA. n. A sensitive immunoassay that uses an enzyme linked to an antibody or antigen as a marker for the detection of a specific protein, especially an antigen or antibody. ELISA. a diagnostic test for detecting exposure to an infectious agent, as the AIDS virus, by combining a blood sample with antigen of the agent and probing with an enzyme that causes a color change when antibody to the infection is present in the sample. 1 Enzyme-linked immunosorbent assay (ELISA) . 2  This test is usually the first one used to detect infection with HIV. 3  If antibodies to HIV are present (positive), the test is usually repeated to confirm the diagnosis. 4  If ELISA is negative, other tests are not usually needed. These tests include: 1  ELISA, or EIA, is an acronym for enzyme-linked immuno assay. ELISA is a test that detects and measures antibodies in your blood. This test can be used to determine if you have antibodies that are related to certain infectious conditions. Antibodies are proteins that the body produces in response to harmful substances (antigens). An ELISA test may be used to diagnose: 1  HIV (the virus that causes AIDS). 2  Lyme disease. ELISA. n. Enzyme-linked immunosorbent assay; a sensitive immunoassay that uses an enzyme linked to an antibody or antigen as a marker for the detection of a specific protein, especially an antigen or antibody. Enzyme-linked immunosorbent assay Lab medicine A heterogeneous immunoenzymatic assay that approaches the sensitivity of RIA Pros Lower cost, simpler equipment, faster 'turn-around time', and none of the problems inherent in handling radioactive substances; ELISA may be used to measure any antigen and antibody. What is ELISA. Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. Enzyme-linked immunosorbent assay (ELISA) ELISA is a popular format of wet-lab type analytic biochemistry assay that uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample or wet sample. This antibody is linked to an enzyme, and, in the final step, a substance containing the enzyme's substrate is added. The subsequent reaction produces a detectable signal, most commonly a color change in the substrate. Performing an ELISA involves at least one antibody with specificity for a particular antigen. Because the ELISA can be performed to evaluate either the presence of antigen or the presence of antibody in a sample, it is a useful tool both for determining serum antibody concentrations (such as with the Human Immunodeficiency Virus, HIV test or West Nile Virus) and also for detecting the presence of antigen. The other reacts to antigen-antibody complexes, and is coupled to an enzyme. This second antibody, which accounts for enzyme-linked in the test's name, can also cause a chromogenic or fluorogenic substrate to produce a signal. An ELISA test uses components of the immune system and chemicals to detect immune responses in the body (for example, to infectious microbes). The ELISA test involves an enzyme (a protein that catalyzes a biochemical reaction). ELISA tests are widely utilized to detect substances that have antigenic properties, primarily proteins (as opposed to small molecules and ions such as glucose and potassium). The substances detected by ELISA tests include hormones, bacterial antigens and antibodies.
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what is the elisa test