10.6092/issn.1973-9494/9991
In this study, conservative strategies are applied in order to limit any irreversible damage and to characterize and quantify microbial colonization that may induce the deterioration of constitutive heritage materials; subsequently, suitable antimicrobial interventions are performed based on the use of biotechnology. This paper presents a summary of the basic and applied biotechnology research developed in the Laboratory of Biology and Biotechnology for Cultural Heritage (LaBBCH, University of Palermo, Italy). It shows that biotechnology has an evidently important role in the preservation and restoration of cultural assets, also taking into consideration that “Prevention is better than cure”, a milestone in the field of human health that can be shifted and applied to the “cure and preservation” of cultural assets. Moreover, biotechnological tools offer great potential for application, thanks to the increasing interaction between the worlds of art and science, thus opening the way for advanced innovation in the conservation and restoration field of art works.
application/pdf
1973-9494
9991
en
Ambra Giordano
Conservation Science in Cultural Heritage
green biocide
Biotechnology a Source of Knowledge in Agreement with Green Strategies for the Conservation of Cultural Assets
Text.Serial.Journal
https://conservation-science.unibo.it/
19
Articles
2020-03-18
2019-11-12
2020-03-18
2020-03-18
69-80
CSCH
Ambra Giordano
2019
10.6092/issn.1973-9494/9991
69
Università degli Studi di Palermo
1973-9494
green biocide
en
80
Biotechnology a Source of Knowledge in Agreement with Green Strategies for the Conservation of Cultural Assets
https://conservation-science.unibo.it/article/download/9991/9771
19
B    IOTECHNOLOGY, A SOURCE OF KNOWLEDGE IN
      AGREEMENT WITH GREEN STRATEGIES FOR THE
      CONSERVATION OF CULTURAL ASSETS
   Franco Palla*,1Valentina Rotolo, Ambra Giordano
   Laboratorio di Biologia e Biotecnologie per i Beni Culturali
   Università degli Studi di Palermo, Italy




   Keywords: biodeterioration, integrated approach, bioactive molecules, green biocide.


   1. Introduction

    The words, “Prevention is better than cure” represent a milestone for human health,
at the same time however, the same words can unquestionably be shifted to the “cure
and preservation” of cultural assets. Indeed, in order to perform adequate strategies,
it is indispensable to limit any irreversible damage, characterize and quantify any mi-
crobial colonization that may induce the deterioration of constitutive heritage materials,
and perform suitable antimicrobial interventions through the use of biotechnology.
    As regards historic-artistic artifacts, biological, chemical and physical factors are
able to act concomitantly to induce the degradation of both organic and inorganic
artworks. The huge number of papers available in scientific literature attest that bio-
technology plays a key role in medicine, agriculture and industry, with its importance
becoming increasingly evident in the preservation and restoration of cultural assets.
    Particularly in the last two decades relevant biotechnological applications in the field
of artwork preservation have emerged opening the way for advanced innovation. This
paper presents a summary of the basic and applied biotechnology research devel-
oped in the Laboratory of Biology and Biotechnology for Cultural Heritage (LaBBCH,
University of Palermo, Italy). In addition, the further development of biotechnological
techniques can be hypothesized, creating new opportunities for biologists, biotech-
nologists and artwork conservators, providing innovative strategies that are totally safe
for artworks, operators and their environment.
    Finally, biotechnological tools have a great potential of application thanks to the
increasing interaction between the worlds of art and science.


*1 Corresponding author: franco.palla@unipa.it
                                                                                             2. Biodeterioration

                                                                                            Several biological systems are able to induce “undesirable change in the properties
                                                                                        of a material caused by the vital activities” (Hueck, 1968). Bacteria and fungi in particu-
                                                                                        lar, that are able to colonize many different nature artworks or present as environmen-
                                                                                        tal aerosol pollutants in relation to their metabolic versatility, represent complex prob-
                                                                                        lems for conservation. Moreover, specific thermo-hygrometric parameters can lead to
                                                                                        higher concentrations of microbial units, enhancing the deterioration process (mainly
                                                                                        due to the release of organic-inorganic acids, enzymes, pigments), altering the chemi-
                                                                                        cal, physical and material properties [1].
                                                                                            Deterioration is also the result of aerosol pollution (dust, grease, soot), resulting in
                                                                                        the deposition of significantly reactive particles on the artwork surface [2, 3]. Frequent-
                                                                                        ly, biological, anthropogenic and pollution factors act simultaneously, thus inducing
                                                                                        the formation of complex stratified layers as shown on the marble sculptured surface
F. Palla, V. Rotolo, A. Giordano - Biotechnology, a source of knowledge in agreement




                                                                                        exposed to outdoor environments in Figure 1.




                                                                                         A                                            B
                                                                                        Figure 1. A complex multilayer on stonework surface, abiotic deposits and biological colonization,
                                                                                        showing different aspects of: A) chromatic and morphological features; B) remarkable depth.



                                                                                           In order to characterize the taxa forming the microbial consortium on the surface of
                                                                                        works of art, an integrated approach (microscopy, in vitro culture, molecular investiga-
                                                                                        tion) is needed [4].
                                                                                           Microscopy observation (using Lugol’s iodine staining), as shown in Figure 2,
                                                                                        revealed fungal reproductive structures, attributable to Aspergillus sp. and Penicil-
                                                                                        lium sp.




                                                                                   70
A                                                B
Figure 2. Microscopy analysis. Fungal structures, hyphae and conidia related to Aspergillus sp.
(A) and Penicillium sp. (B), observed after staining by Lugol’s iodine reagent.



    A preliminary identification of fungal species was performed by in vitro culture on Sa-
bouraud media, allowing the identification of the morphological profile of Aspergillus fla-
vus, Aspergillus niger, Alternaria alternata, Penicillium chrisogenum colonies (Figure 3).
    The third step in the integrated approach is molecular investigation, which con-
tributes to confirming and completing the overview of the microbial colonizers. Spe-




                                                                                                          CONSERVATION SCIENCE IN CULTURAL HERITAGE
cifically, the sequencing and sequence analysis of the DNA fragments, as products of
the polymerase chain reactions (Figure 4), allow us to confirm the fungal species of
Aspergillus flavus, Aspergillus niger, Alternaria alternata, Penicillum chrisogenum, as
well as Alternaria brassicicola.




Figure 3. In vitro culture. Fungal colonies grown on Sabouroud-agar plate: (A) Aspergillus flavus;
(B) Aspergillus niger; (C) Alternaria alternate; (D) Penicillium chrisogenum.


                                                                                                     71
F. Palla, V. Rotolo, A. Giordano - Biotechnology, a source of knowledge in agreement




                                                                                        Figure 4. In vitro amplification of ITS rDNA region, using as a template the genomic microbial
                                                                                        DNA directly extracted from biofilm. M = 100 bp DNA ladder; NC= negative-control; PCR reaction
                                                                                        products corresponding to: A= Alternaria alternata; B=Aspergillus niger; C=Aspergillus flavus;
                                                                                        D=Penicillium chrisogenum; E= Alternaria brassicola



                                                                                           3. Bioaerosol

                                                                                            The identification of the largest number of bacteria and fungi has a marked impor-
                                                                                        tance, also from the point of view of risk management in cultural heritage environments
                                                                                        (CHE), specifically due to the presence of biological particles (allergens, cellular par-
                                                                                        ticles, spores, toxins), potentially dangerous for operator and visitor health [5 – 10].
                                                                                        As shown in Figure 5, an active sampling procedure of the CHE aerosol is outlined.
                                                                                        Biological particles are collected using a Sartorius MD8 portable air sampler, equipped
                                                                                        with sterile gelatine membrane filter.
                                                                                            The peculiarity of gelatine membrane filters is their solubility in aqueous solu-
                                                                                        tions so, in addition to being used to inoculate agar media, it is possible to dissolve
                                                                                        them in solution as 1xTE = 10mM Tris-HCl pH 7.5 /1 mM EDTA, performing the
                                                                                        direct extraction of the microbial genomic DNA. The DNA will later be the template
                                                                                        molecules in PCR reactions. Figure 5 schematically describes the integrated pro-
                                                                                        cedure utilized to reveal and identify microbial taxa colonizing the aerosol of CHE,
                                                                                        such as archives.




                                                                                   72
Figure 5. Scheme for analysis of CHE aerosol. Sampling by MD8 portable sampler equipped
with gelatine membrane filter (A), the microbial taxa are identified combining an in vitro culture
on Nutrient (B) or Sabouraud (C) agar media, and molecular investigation using the genomic
microbial DNA (D) as a template in PCR reactions amplifying ITS rDNA regions (E, F).




                                                                                                          CONSERVATION SCIENCE IN CULTURAL HERITAGE
   4. Molecular biology investigation

    As concerns genus/species identification, the molecular biology approach, based
on the analysis of specific DNA genomic sequences was methodically applied to iden-
tify microbial colonizers on both inorganic and organic materials, exposed/stored in
museums, archive libraries, deposits, etc. [11, 12]. Particularly by using the polymer-
ase chain reaction (PCR) technique, an important tool for rapid identification of the
biological systems, specific genomic regions can be in vitro amplified.
    The corresponding amplification products are analysed through gel electrophoresis,
followed by the resolving of the nucleotide composition (sequencing) and related anal-
yses of sequence homology by consulting international nucleotide databases (e.g.,
NIH, USA; EMBL, Germany), performing phylogenetic analysis and also identifying
novel microbial species [13, 14].
    From our point of view, molecular investigation must be considered as part of
an interdisciplinary approach, where the contribution of microscopy (Optical, SEM,
CLSM) and in vitro culture (Nutrient / Sabouraud agar) are of comparable importance
[9, 15-17].




                                                                                                     73
                                                                                           5. Biological colonization control

                                                                                           The control of microbial colonization is generally performed by powerful biocides,
                                                                                        chemical compounds with a broad spectrum of action against Gram+/- bacteria, brown
                                                                                        and green algae, lichens, molds, micro-fungi [18-20]. The toxicity of many synthetic
                                                                                        products is well understood [21] and some of them are utilized in preventive conserva-
                                                                                        tion or restoration procedures.
                                                                                           Recently, natural compounds (such as essential oils) have been tested to replace
                                                                                        chemical compounds in order to develop alternative methods to control microbial colo-
                                                                                        nization and to prevent repeated occurrences [5, 23-29].
                                                                                           The in vitro evaluation of antimicrobial activity can be performed by means of at
                                                                                        least three methods defined as: agar disk diffusion (Fig. 6), well diffusion or microdilu-
                                                                                        tion [5, 22, 29-32].
                                                                                           Performing the agar disk diffusion assay, a paper disk (6 mm in diameter, wetted
F. Palla, V. Rotolo, A. Giordano - Biotechnology, a source of knowledge in agreement




                                                                                        with 10 μl of essential oils (at different concentrations: from 6.25 to 100%) is placed
                                                                                        on the surface of nutrient or Sabouraud agar plates (90 mm Petri dish) inoculated
                                                                                        by a bacterial or fungal single colony culture at a concentration of 1×106 CFU/
                                                                                        ml or 1x104 conidia/ml [5]. After incubation for 24/48 hours at 30 ± 1ºC, confluent
                                                                                        microbial growth was observed and the diameter (mm) of growth inhibition areas
                                                                                        measured, as shown in Figure 6. The measurement of the growth inhibition halo
                                                                                        (diameter in mm) allows the definition of antimicrobial activity (sensible > 9 mm,
                                                                                        resistant < 6 mm).




                                                                                        Figure 6. Agar disk diffusion method to test the antimicrobial activity of Thuja plicata Donn.
                                                                                        essential oil (EO) vs Bacillus subtilis colonies. The inhibition halos are related to 6.25% (left) and
                                                                                        25% (right) EO. The red lines highlight that T. plicata EO already has antimicrobial activity at a
                                                                                        lower percentage (6.25%).




                                                                                   74
   A high susceptibility of microbial strains to Thuja plicata Donn. essential oil was also
revealed for Stafilococcus aureus (Figure 7A) and Penicillium chrysogenum (Figure 7B).




Figure 7. Agar disk diffusion method. The antimicrobial activity of Thuja plicata EO vs Stafilococcus
aureus (A) and Penicillium chrysogenum colonies (B) is evident for both EO concentrations (6.25
and 25%).



   Furthermore, Thuja plicata EO (12.5, 25% and 50%) showed peculiar growth inhibi-
tion activity also against Micrococcus luteus colonies; the antimicrobial activity was so




                                                                                                             CONSERVATION SCIENCE IN CULTURAL HERITAGE
high that the inhibition halos were confluent (Figure 8).




Figure 8. Agar disk diffusion method. Evident antimicrobial activity of Thuja plicata EO is shown
vs Micrococcus luteus colonies.



                                                                                                        75
                                                                                            In order to achieve information useful in comparing the sensibility of microbial spe-
                                                                                        cies tested in this work to a commercial biocide, Benzalkonium Chloride (BC) was uti-
                                                                                        lized in control assays. Specifically, a paper disk was wetted with 6.25% BC and after
                                                                                        incubation at 30°C for 24/48 h, confluent microbial growth was observed except in the
                                                                                        growth inhibition area (halo), Figure 9 [33, 34].
F. Palla, V. Rotolo, A. Giordano - Biotechnology, a source of knowledge in agreement




                                                                                        Figure 9. Agar disk diffusion method, control assays. Antimicrobial activity of Benzalkoniun
                                                                                        chloride vs Micrococcus luteus (A) or Bacillus subtilis (B) colonies.



                                                                                           6. Conclusions

                                                                                           Microorganisms play a significant role in the biodeterioration of cultural assets and,
                                                                                        depending on growth processes, development and metabolic activities can cause
                                                                                        physical-chemical alterations and aesthetic damage to works of art with strong nega-
                                                                                        tive consequences for conservation strategies [35].
                                                                                           In this study we suggest an integrated approach to detect and identify the microor-
                                                                                        ganisms colonizing artwork surfaces and the aerosol of cultural heritage environments,
                                                                                        highlighting that molecular techniques definitely improve the sensitivity and specificity
                                                                                        of diagnostic investigation.
                                                                                           The potential use of commercial (Melaleuca alternifolia Origanum vulgare, Thy-
                                                                                        mus vulgaris, Thuja plicata) or laboratory distilled (Allium sativum, Calamita ne-
                                                                                        peta, Crithmum maritmum,) plant essential oils, together with the achievements
                                                                                        reached during the in vitro and in situ applications to control the growth of bacterial
                                                                                        (Bacillus subtilis, Micrococcus luteus, Stafilococcus aureus) or fungal (Alternaria
                                                                                        alternata, Alternaria brassicola, Aspergillus flavus, Aspergillum niger, Penicillium
                                                                                        chrysogenum) taxa, have led us to hypothesize their use as natural biocides, also
                                                                                        focusing on their importance in safeguarding the environment and human health
                                                                                        [27, 29]. Consistent with the hypothesis, encouraging results have recently been
                                                                                        obtained using Origanum vulgare essential oil in the control of complex biofilm
                                                                                        growth beneath mosaic tesserae [33].

                                                                                   76
      Acknowledgments

   The Authors thank prof. Maurizio Bruno for supplying the distilled essential oils (Al-
lium sativum, Calamita nepeta L., Crithmum maritmum) and the Institutions devoted to
the conservation of cultural heritage in Sicily.


      References

[1]  Di Carlo E, Barresi G, Palla F (2017) Biodeterioration. In: Palla F, Barresi G.
     (eds), Biotechnology and Conservation of Cultural Heritage, Springer Interna-
     tional Publishing Switzerland: pp. 1-30
[2] Zanardini E, Abbruscato P, Ghedini N, Realini M, Sorlini C (2000) Influence of atmos-
     pheric pollutants on the biodeterioration of stone. Int Biodeter Biodegr 45(1–2):35–42.
[3] Spiridon P, Sandu I, Stratulat L (2017) The conscious deterioration and degrada-
     tion of the cultural heritage. Int J Conservation Science 8 (1): 81-88
[4] Palla F & Barresi G (2017) Biotechnology and Conservation of Cultural Heritage.
     Springer International Publishing, Cham, Switzerland
[5] Borrego S, Lavin P, Perdomo I, Gómez de Saravia S, Guiamet P (2012) Deter-
     mination of indoor air quality in archives and biodeterioration of the documentary
     heritage. ISRN Microbiol 2012: 1–10
[6] Šimonovičová A, Kraková L, Pangallo D, Majorošová M, Piecková E, Bodoriková
     S, Dörnhoferová M (2015) Fungi on mummified human remains and in the indoor
     air in the Kuffner family crypt in Sládkovičovo (Slovakia). Int Biodeter Biodegrad
     99:157–164
[7] Palla F (2015) Biological risk for the conservation and exhibition of historical–
     artistic artefacts in confined spaces. In: Lorusso S, Natali A, Palla F (eds) Risk
     management in the Cultural Heritage sector: Museums, libraries and archives.




                                                                                                    CONSERVATION SCIENCE IN CULTURAL HERITAGE
     Mimesis Edizioni, Milano-Udine, pp 95–108
[8] Palla F, Di Carlo E, Barresi G, Billeci N, Chisesi R, Barbaro S, Sebastianelli S
     (2014) Bioaerosol in indoor environments for conservation of cultural heritage:
     potential risks for historical-artistic manifacts and human health. In: International
     Conference Indoor Air Quality in Heritage and Historic Environments, Prague,
     Kaiserštejn Palace, April 13–16 2014, p 47
[9] Di Carlo E, Chisesi R, Barresi G, Barbaro S, Lombardo G, Rotolo V, Sebastianelli
     M, Travagliato G, Palla F (2016) Fungi and bacteria in indoor Cultural Heritage
     environments: microbial-related risks for artworks and human health. Environ
     Ecol Res 4(5):257–264.
[10] De Nuntiis P, Palla F (2017) Bioerosol. In Palla F & Barresi G (eds) Biotech-
     nology and Conservation of Cultural Heritage. Springer International Publishing,
     Cham, Switzerland, pp. 31-48
[11] Piñar G, Garcia-Valles M, Gimeno-Torrente D, Fernandez-Turiel JL, Ettenauer J,
     Sterflinger K (2013) Microscopic, chemical, and molecular-biological investiga-
     tion of the decayed medieval stained window glasses of two Catalonian church-
     es. Int Biodeterior Biodegrad 84 (100): 388-400
[12] Valentin N (2003) Microbial contamination in museum collections: organic mate-
     rials. In: Saiz-Jimenez C (ed) Molecular biology and Cultural Heritage. Swets &
     Zeitlinger, Lisse


                                                                                               77
                                                                                        [13] González JM, Saiz-Jiménez C (2004) Microbial diversity in biodeteriorated
                                                                                             monuments as studied by denaturing gradient gel electrophoresis. J Sep Sci
                                                                                             27(3):174–180
                                                                                        [14] Gaüzère C, Moletta-Denat M, Blanquart H, Ferreira S, Moularat S, Godon J-J,
                                                                                             Robine E (2014) Stability of airborne microbes in the Louvre Museum over time.
                                                                                             Indoor Air 24:29–40
                                                                                        [15] Palla F, Billeci N, Mancuso FP, Pellegrino L, Lorusso LC (2010) Microscopy and
                                                                                             molecular biology techniques for the study of biocenosis diversity in semi-con-
                                                                                             fined environments. Conserv Sci Cult Heritage 10:185–194
                                                                                        [16] Palla F, Mancuso FP, Billeci N. (2013) Multiple approach to identify bacteria in
                                                                                             archaeological waterlogged wood. J Cult Heritage 14: e61–64
                                                                                        [17] Pasquarella C, Pasquariello G, Balocco C, Saccani E, Marmonti E, Palla F,
                                                                                             Ugolotti M, Maggi O, Albertini R (2015) Biological and microclimatic diagnosis
                                                                                             in cultural heritage conservation: interdisciplinary research at Palatina library in
F. Palla, V. Rotolo, A. Giordano - Biotechnology, a source of knowledge in agreement




                                                                                             Parma. Sci Tot Environ 536:557–567
                                                                                        [18] Marshall BM, McMurry LM (2005) Biocides and resistance. In: White DG, Aleks-
                                                                                             hun MN, McDermott PF (eds) Frontiers in Antimicrobial Resistance: a Tribute to
                                                                                             Stuart B. Levy. ASM Press, Washington, D.C., pp 174–190
                                                                                        [19] Martin-Sanchez PM, Nováková A, Bastian F, Alabouvette C, Saiz-Jimenez
                                                                                             C (2012) Use of biocides for the control of fungal outbreaks in subterranean
                                                                                             environments: the case of the Lascaux Cave in France. Environ Sci Technol
                                                                                             46(7):3762–3770
                                                                                        [20] Maxim D, Bucşa L, Moza MI, Chachula O (2012) Preliminary antifungal investi-
                                                                                             gation of ten biocides against moulds from two different church frescos. Ann Rom
                                                                                             Soc Cell Biol 17(2):139
                                                                                        [21] Coors A, Vollmar P, Heim J, Sacher F, Kehrer A (2018) Environmental risk as-
                                                                                             sessment of biocidal products: identification of relevant components and reliabil-
                                                                                             ity of a component-based mixture assessment. Environ Sci Eur. 2018; 30(1): 3
                                                                                        [22] Guiamet PS, De la Paz JN, Arenas PM, Gómez de Saravia SG (2008) Differ-
                                                                                             ential sensitivity of Bacillus sp. isolated from archive materials to plant extracts,
                                                                                             Pharmacologyonline 3: 649-658
                                                                                        [23] Sakr AA, Ghaly MF, Abdel-Haliem M (2012) The efficacy of specific essential oils
                                                                                             on yeasts isolated from the royal tomb paintings at Tanis, Egypt. International
                                                                                             Journal of Conservation Science 3(2): 87-92
                                                                                        [24] Walentowska J, Foksowicz-Flaczyk J (2013) Thyme essential oil for antimicrobial
                                                                                             protection of natural textiles. Int Biodeter Biodegr 84: 407–411
                                                                                        [25] Marin E, Vaccaro C, Leis M (2016) Biotechnology applied to historic stonework
                                                                                             conservation: testing the potential harmfulness of two biological biocides. Inter-
                                                                                             national J Conservation Science 7 (1): 227-238
                                                                                        [26] Noshyutta W, Osman E, Mansour M (2016) An investigation of the biological fungi-
                                                                                             cidal activity of some essential oils used as preservatives for a 19th century Egyp-
                                                                                             tian Coptic cellulosic manuscript. International J Conservation Science 7 (1): 41-56
                                                                                        [27] Rotolo V, Barresi G, Di Carlo E, Giordano A, Lombardo G, Crimi E, Costa E, Bruno
                                                                                             M, Palla F (2016) Plant extracts as green potential strategies to control the biode-
                                                                                             terioration of cultural heritage. International J Conservation Science 7 (2): 839-846
                                                                                        [28] Salem MZM, Zidan YE, Mansour MMA, El Hadidi NMN (2016) Antifungal activi-
                                                                                             ties of two essential oils used in treatment of three commercial woods deterio-
                                                                                             rated by five common mold fungi. Int Biodet Biodegr 106: 88–96


                                                                                   78
[29] Barresi G, Cammarata M, Palla F (2017) Biocides. In: Palla F, Barresi G. (eds),
     Biotechnology and Conservation of Cultural Heritage, Springer International
     Publishing Switzerland: pp. 49-65
[30] Domig KJ, Mayrhofer S, Zitz U, Mair C, Petersson A, Amtmann E, Mayer EK,
     Kneifel W (2007) Antibiotic susceptibility testing of Bifidobacterium thermophilum
     and Bifidobacterium pseudolongum strains: Broth microdilution vs. agar disc dif-
     fusion assay. Int J Food Microbiol 120:191–195
[31] Gómez de Saravia SG, de la Paz Naranjo J, Guiamet P, Arenas P, Borrego S
     (2008) Biocide activity of natural extracts against microorganisms affecting ar-
     chives. Bol Latinoam Caribe Plant Med Aromat 7(1):25–29
[32] Balouiri M, Sadiki M, Ibnsouda SK (2016) Methods for in vitro evaluating antimi-
     crobial activity: a review. J Pharm Anal 6(2):71–79
[33] Rotolo V, De Caro ML, Giordano A, Palla F (2018) Solunto archaeological park in
     Sicily: life under mosaic tesserae. Fl Medit 28: 233-245
[34] Stupar M, Ljaljević-Grbić M, Džamić A, Unković N, Ristić M, Jelikić A, Vukojević
     JB (2014) Antifungal activity of selected essential oils and biocide benzalkonium
     chloride against the fungi isolated from cultural heritage objects. South African
     Journal of Botany 93: 118–124
[35] Fort, R., Alvarez de Buergo, M., Gomez-Heras, M., Vazquez-Calvo, C. (2006)
     Heritage, Weathering and Conservation vol 1-2. – London


  Biographical notes

    Franco Palla is Associate Professor of Applied and Environmental Botany at the
University of Palermo, Italy. He is Professor and Coordinator of the five-year Degree in
Conservation and Restoration of Cultural Property (MRL02, certified professional re-
storer). He was the scientific head of the UNIPA Research Unit, for the Research Pro-




                                                                                                 CONSERVATION SCIENCE IN CULTURAL HERITAGE
ject PON01_00625, It@cha (Italian Technology for Advanced Applications in Cultural
Assets) and was one of the members of the working groups in the joint project between
Italy-Cambodia for Training Experts of Cultural Heritage, University of Palermo – Royal
University of Fine Arts and the Ministry of Culture and Fine Arts, Angkor, Cambodia.
He is the Coordinator of the Laboratory of Biology and Biotechnologies for Cultural
Heritage at the Department of Biological, Chemical and Pharmaceutical Sciences and
Technologies (STEBICEF) of the University of Palermo in Italy, performing applied
research and scientific consulting in the field of the conservation and restoration of
Sicilian cultural heritage.

    Valentina Rotolo graduated in “Biological Science” at the University of Palermo (Ita-
ly) and specialized in “Microbiology and Virology” at the School of Medicine in the same
university. She was a Member of the Commission “Protection of Cultural Heritage” of
the “National Order of Biologists”. She carries out research at the Laboratory of Biology
and Biotechnology for the Cultural Heritage (LABBBC) of the Department of Biological,
Chemical and Pharmaceutical Sciences and Technologies (STEBICEF) at the Univer-
sity of Palermo, where she is in charge of environmental monitoring and microbiologi-
cal diagnosis in storage environments and use of cultural heritage and the search for
natural methods to control microbial growth on artefacts. She is currently professor of
“Elements of Biology applied to Restoration” at the Academy of Fine Arts in Palermo.


                                                                                            79
                                                                                           Ambra Giordano graduated in “Conservation and Restoration of Cultural
                                                                                        Heritage” at the University of Palermo in Italy. She has been Vice President of the
                                                                                        CESMAR7 “Research Center for Conservation Materials” in Verona. From 2014
                                                                                        she has carried out research at the Laboratory of Biology and Biotechnology for
                                                                                        the Cultural Heritage (LABBBC) of the Department of Biological, Chemical and
                                                                                        Pharmaceutical Sciences and Technologies (STEBICEF) at the University of
                                                                                        Palermo, dealing with innovative materials and techniques for the conservation and
                                                                                        restoration of works of art. She currently teaches “Restoration of Contemporary
                                                                                        Art” at the Academy of Fine Arts in Palermo.


                                                                                           Summary
                                                                                           In this study, conservative strategies are applied in order to limit any irreversible
                                                                                        damage and to characterize and quantify microbial colonization that may induce the
F. Palla, V. Rotolo, A. Giordano - Biotechnology, a source of knowledge in agreement




                                                                                        deterioration of constitutive heritage materials; subsequently, suitable antimicrobial in-
                                                                                        terventions are performed based on the use of biotechnology.
                                                                                           This paper presents a summary of the basic and applied biotechnology research
                                                                                        developed in the Laboratory of Biology and Biotechnology for Cultural Heritage
                                                                                        (LaBBCH, University of Palermo, Italy). It shows that biotechnology has an evidently
                                                                                        important role in the preservation and restoration of cultural assets, also taking into
                                                                                        consideration that “Prevention is better than cure”, a milestone in the field of human
                                                                                        health that can be shifted and applied to the “cure and preservation” of cultural assets.
                                                                                           Moreover, biotechnological tools offer great potential for application, thanks to the
                                                                                        increasing interaction between the worlds of art and science, thus opening the way for
                                                                                        advanced innovation in the conservation and restoration field of art works.


                                                                                            Riassunto
                                                                                            In questo studio sono presentate alcune strategie conservative per caratterizzare e
                                                                                        quantificare la colonizzazione microbica che può indurre il deterioramento dei materiali
                                                                                        costitutivi di manufatti d’interesse storico-artistico, basate su protocolli biotecnologici.
                                                                                            Qui è presentata una sintesi della ricerca biotecnologica di base e applicata, con-
                                                                                        dotta nel Laboratorio di Biologia e Biotecnologie per i Beni Culturali (LaBBCH). Ciò
                                                                                        dimostra l’evidente ruolo della biotecnologia nella conservazione e nel restauro di beni
                                                                                        culturali, sottolineando che “Prevenire è meglio che curare”, pietra miliare per la salute
                                                                                        umana che può essere rivolta alla “cura e conservazione” dei beni culturali.
                                                                                            Inoltre, gli strumenti biotecnologici hanno un grande potenziale di applicazione gra-
                                                                                        zie alla crescente interazione tra il mondo dell’arte e della scienza, aprendo la strada
                                                                                        all’innovazione avanzata nel campo della conservazione / restauro di opere d’arte.




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