10.6092/issn.2531-7342/12562
We report the identification of both Hydnum ibericum and H. ovoideisporum growing in late autumn, in the woods of Monte Rocca Romana, the highest peak (612 m) of Monti Sabatini, inside the Bracciano-Martignano Regional Natural Park in Latium, Italy. The Park includes large composite zones: the relatively humid climate and the fertile volcanic soils allow the growth of a manifold flora related to the Mediterranean scrub, to the deciduous (Quercus spp.) or chestnut (Castanea sativa) and depressed beech woods. The fungal findings were observed in an area of the Park characterised by a forest composed in prevalence of chestnut trees and underwood of Ilex aquifolium and Ruscus aculeatus. In the same habitat and period, two small fungal species were present: blue coloured Entoloma sp. and Ramariopsis pulchella. The Entoloma sp. belongs to the Cyanula subgenus but combined morphological and molecular criteria did not correspond to any described Entoloma species yet. Ramariopis pulchella is a rare and ecologically important species.
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Rare findings from the chestnut forest of Monte Rocca Romana (Latium, Italy)
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Volobuev S, Bolshakov S (2017) Host preferences and distributional patterns of rare and protected species of aphyllophoroid fungi in the Middle Russian Upland. In: Proceedings of the conference Fungal conservation in a changing Europe (International Society for Fungal Conservation Ss. Cyril and Methodius University, Skopje, Macedonian Mycological Society, ed), 1-6 October 2017, Ohrid, Republic of Macedonia, p 61.
Rare findings from the chestnut forest of Monte Rocca Romana (Latium, Italy)
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                 IJM - Italian Journal of Mycology
                 ISSN 2531-7342 - Vol. 50 (2021): 78-91
                 Journal homepage: https://italianmycology.unibo.it/

Original paper

                         Rare findings from the chestnut forest
                        of Monte Rocca Romana (Latium, Italy)

                               Arnold Knijn1, Amalia Ferretti1, Irja Saar2

1 Via Arno 36, 00198 Roma, Italy
2 Institute of Ecology and Earth Sciences, University of Tartu, Ravila 14A, 50411 Tartu, Estonia

* Corresponding author e-mail: knijn_roma@hotmail.com


Article info
Received 18/3/2021; accepted 18/6/2021
https://doi.org/10.6092/issn.2531-7342/12562

Abstract
We report the identification of both Hydnum ibericum and H. ovoideisporum growing in late autumn, in the woods of
Monte Rocca Romana, the highest peak (612 m asl) of Monti Sabatini, inside the Bracciano-Martignano Regional Natural
Park in Latium, Italy. The Park includes large composite zones: the relatively humid climate and the fertile volcanic soils
allow the growth of a manifold flora related to the Mediterranean scrub, to the deciduous (Quercus spp.) or chestnut
(Castanea sativa) and depressed beech woods. The fungal findings were observed in an area of the Park characterised by
a forest composed in prevalence of chestnut trees and underwood of Ilex aquifolium and Ruscus aculeatus. In the same
habitat and period, two small fungal species were present: blue coloured Entoloma sp. and Ramariopsis pulchella. The
Entoloma sp. belongs to the Cyanula subgenus but combined morphological and molecular criteria did not correspond to
any described Entoloma species yet. Ramariopis pulchella is a rare and ecologically important species.

Keywords
Hydnum ibericum; Hydnum ovoideisporum; Entoloma; Cyanula; Ramariopsis pulchella; chestnut forest; Natural Parks


Introduction
The genus Hydnum L. (Basidiomycetes, Agaricomycetes, Cantharellales, Hydnaceae) was originally
composed of six species present in both Europe and North America, on the basis of morphological
characteristics: H. repandum L., H. rufescens Pers., H. albidum Peck, H. umbilicatum Peck H.
ellipsosporum Ostrow & Beenken and H. albomagnum Banker (Ostrow and Beenken, 2004; Grebenc
et al., 2009; Swenie et al., 2018). Various subsequent phylogenetic studies have raised this number
currently to 34 global species (Feng et al., 2016; Buyck et al., 2017; Niskanen et al., 2018; Swenie
et al., 2018). For example, the two species H. ovoideisporum and H. vesterholtii Olariaga, Grebenc,
Salcedo & M.P. Martín were described as new taxa (Olariaga et al., 2012). Recently, another new
species was defined: H. ibericum Olariaga, Liimat. & Niskanen, sister species of H. vesterholtii from
which it deviates by eight substitution and indel positions in the genomic ITS sequences (Niskanen
et al., 2018). Nonetheless, Hydnum remains one of the most understudied fungal genera (Feng et al.,
2016). In fact, only 45 specimens of the genus have been officially recorded for Italy (https://www.
gbif.org/species/2513763, consulted on 2021/05/15).
    Hydnum ibericum is described as characterised by a small pileus, with cream or light ochre colours
and orange tones, the hymenium with concoloured spines and ellipsoidal basidiospores. Hydnum

Department of Agricultural and Food Science in partnership with the Italian Mycological Society (UMI).
The journal is hosted and mantained by ABIS-AlmaDL. © 2021 The Authors. This work is licensed under CC BY 4.0
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ibericum is terricolous and ectomycorrhizal, like the other species of the genus (Agerer et al., 1996).
It appears during late autumn and winter in coniferous forests and with Castanea sativa Mill. in Spain
(Niskanen et al., 2018). Hydnum vesterholtii is characterised by an ochre to light ochre pileus with
white stipe, spines slightly or not decurrent, sharply delimitated and conical not flattened, with pale
ochre colour. It harbours ovoid to broadly ellipsoid basidiospores (Olariaga et al., 2012). Initially
observed only in southern Europe, the Pyrenees and Andorra (Olariaga et al., 2012), H. vesterholtii
was reported to be present also in Estonia (Vizzini et al., 2013). Hydnum ovoideisporum is described
to accommodate brownish and deep orange pileus with conical and non-flattened spines, from orange-
white to orange-ochre colours. The spines are non-decurrent, but in some cases small decurrent spines
form a circular zone around the stipe. Its basidiospores vary from subglobose to broadly ellipsoid
(Olariaga et al., 2012; Vizzini et al., 2013). This species appears in forests under Pinus sylvestris L.
but also in Quercus woods, in Mediterranean areas of Europe and Italy (Olariaga et al., 2012; Vizzini
et al., 2013).
    We report the finding of H. ibericum and H. ovoideisporum species present in late autumn and
winter, in a forest dominated by Castania sativa on the mountain slope of Monte Rocca Romana, the
highest peak (612 m asl) of the Monti Sabatini, part of the Bracciano-Martignano Regional Natural
Park, in the province of Viterbo, Latium, Italy (Supplementary Fig. 1).
    The establishment of the Park in 1999 was aimed at promoting the conservation and enhancement
of the territory and the natural and cultural resources of the vast area of the Sabatini Mountains. In
fact, despite the past urban expansion, the Park still preserves stretches of riparian vegetation and
extensive forest areas. The keeping of these habitats allows the presence of numerous species of flora
and fauna, which should be a reason for continuous surveillance.
    In the same habitat and season other two small and beautiful fungal species were found and
described: blue coloured Entoloma sp., which determination through phylogenetic analyses will be
discussed and Ramariopsis pulchella (Boud.) Corner, in the family of Clavariaceae, considered a
rare and endangered species, enough to suggest inclusion in a fungal Red List (Onofri et al., 2005).
Just two specimens of R. pulchella have been officially recorded for Italy (https://www.gbif.org/
species/2540204, consulted on 2021/05/15) on the island of Sardinia, although it has also been
reported in Tuscany (Salerni and Perini, 2007). Hence the importance of a continuous survey for rare
and endangered macrofungi in the area.

Materials and Methods
Area of study and samples
The Monti Sabatini are a mountainous group originating from a currently quiescent volcanic district,
which landscape is characterised by crater lakes and wooded hills, near the city of Rome, but partly
extended into the province of Viterbo. The area is included in the Bracciano-Martignano Regional
Natural Park and covered with vegetation characteristic of the Mediterranean forest and the Apennine
beech woods, displaying the particular morphology of fertile volcanic soil in a relatively humid and
rainy climate. The chemical compositions of the Sabatini volcanic products generally fall within
the potassium series KS. The volcanic soils examined in the territory have a low content of calcium
carbonate and a subacid to neutral reaction (pH = 5.3-7.8) and in general are fertile but fragile
pedoenvironments. From the analysis of temperatures and rainfall, it emerges that the Bracciano-
Martignano area figures as a transition zone from a more distinctly Mediterranean climate to a
temperate climate, typical of the inland areas of the Lazio Apennines (Various authors, 2009).
   The highest peak of 612 m asl is Monte Rocca Romana, a geological emergence formed by
volcanic scoria cones, prevalently covered by Quercus cerris L. and other Quercus spp. on the south
slope facing the lake. Instead, C. sativa grows on the more humid slope facing north confining with


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cultivations of hazelnut trees (Corylus avellana L.). Besides the prevalent chestnut trees, these woods
(Supplementary Fig. 2) are composed of Acer campestre L., Ostrya carpinifolia Scop. and sporadic
Populus sp., Ulmus sp. and Fagus sylvatica L. trees; the underwood is formed prevalently by holly
(Ilex aquifolium L.) and Ruscus aculeatus L. Even if part of the pure chestnut woods is the result of
human intervention, C. sativa is considered a representative species of the autochthonous flora of the
district. In this study, the surveyed area stretched about 125×535 m along the escarpment at the side
of the road cutting the C. sativa woods (Supplementary Fig. 1).
    The first Hydnum specimen with orange colourings was collected on December 29th 2019, while in
October and November of 2020 two morphotypes of Hydnum were found, one group with cream-light
ochre colours and the other with orange colours (Fig. 1 and Fig. 3). The other two findings, Entoloma
sp. (subgenus Cyanula) and Ramariopsis pulchella, were present in late autumn in 2020 in the same
habitat on the berm of the road as the Hydnum specimens. Ramariopsis pulchella grew amidst moss,
terricolous but with saprotrophic behaviour.
    All specimens are conserved as part of the Mycological Collections of the Natural History Museum
and Botanical Garden of the University of Tartu, Estonia (Table 1).

           Table 1 - The collected specimen data, their herbarium accession number and nuclear ribosomal
           DNA sequence accession number. All specimens were collected in the same habitat in the woods
           of Monte Rocca Romana.

             Species                    Fungarium codes         UNITE Accession         Collection date
             Hydnum ibericum               TUF135543              UDB0780327              2019/12/29
             Hydnum ibericum               TUF105918              UDB0799298              2020/11/02
             Hydnum ibericum               TUF105948              UDB0799303              2020/11/30
             Hydnum ovoideisporum          TUF105919              UDB0799299              2020/11/02
             Hydnum ovoideisporum          TUF105947              UDB0799302              2020/11/30
             Entoloma sp.                  TUF105920              UDB0799300              2020/11/02
             Ramariopsis pulchella         TUF105921              UDB0799301              2020/11/02

Molecular analyses
Ribosomal DNA-based analysis was performed on all specimens listed in Table 1 in the frame of
the UNITE project (Kõljalg et al., 2013; https://unite.ut.ee/). DNA extraction, PCR amplification of
SSU partial, ITS1, 5.8S, ITS2, LSU partial regions and sequencing were performed as in Voitk et al.,
(2018). BLAST 2.8.1+ (Camacho et al., 2009) was used to assess sequence identity, when possible,
and to select sequences for follow-up phylogenetic analyses. The selected sequences were aligned
with the MUSCLE v3.8.425 algorithm (Edgar, 2004) in AliView 1.26 software (Larsson, 2014) and
inspected manually. For the Hydnum sequences, two analyses were performed: one more extensive
with 232 sequences (5 of this study, 2 as outgroup, 225 others, similar to Feng et al., 2016; sequences
in Supplementary Table 1) and another more restrictive with 50 sequences (5 of this study, 1 as
outgroup, 44 others; sequences in Supplementary Table 1). Only the second tree is shown here (the
first tree is present in the Supplementary Fig. 3). For the analysis of the Entoloma sequence a total of
72 sequences were included (1 of this study, 2 as outgroup, 69 others; sequences in Supplementary
Table 2). Maximum Likelihood and Bayesian inference were engaged, applying GTR as the model
for nucleotide evolution after MrModeltest 2.3 (Nylander, 2004). In PhyML 3.1 (Guindon et al.,
2010), Nearest Neighbour Interchange (NNI) was set up as topology research, initial trees obtained
applying BIONJ and Neighbour-Joining to a matrix of pairwise distances. In MrBayes 3.2.6 (Ronquist

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et al., 2012), the Markov Chain Monte Carlo algorithm was run using four chains (three hot and one
cold), setting a temperature of 0.1 and iterations for 2×106 generations on two trees with a diagnose
frequency every 5,000 generations and a 25% burn-in. The final mean standard deviation of the
separated frequencies was below 0.008 for both analyses of Hydnum spp. and Entoloma sp. multiple
sequence alignments. The phylogenetic trees were produced using FigTree v1.4.3. The seven newly
obtained sequences were submitted to the UNITE database (accession numbers in Table 1).

Morphological analyses
The photographs of basidiomata were taken in the field, apart from the Hydnum specimen collected
in December 2019 (Fig. 1). Microscopic images were either obtained with a digital camera AmScope
(Irvine CA, USA) MU 500 mounted on an AmScope B490 microscope, operating at ×800 and ×2000
magnifications or on a Nikon Eclipse Ni microscope, at ×2000 magnifications, with software NIS-
Elements BR (Nikon Corporation, Tokyo, Japan). The mean length and width of free basidiospores
were measured in lateral view.

Results
Upon the observation of Hydnum fungi with an adnate hymenium in 2019, which genomic and
phylogenetic analyses proved to be H. ibericum, a further investigation of the area was performed
in 2020. Various fungal species were present in late autumn and winter, in the same habitat together
with reappeared Hydnum specimens under C. sativa woods, among which Cortinarius, Macrolepiota
and Helvella species, Geoglossum umbratile Sacc., Astraeus hygrometricus (Pers.) Morgan, and
Strobilomyces strobilaceus (Scop.) Berk., various Clavariaceae and others. Astreus hygrometricus,
and S. strobilaceus were proposed to be inserted in the Global Fungal Red List. Two findings in
particular were noteworthy and are described here: Entoloma sp. (subgenus Cyanula) and Ramariopsis
pulchella.

Hydnum spp.
The first finding of this genus in 2019 grew isolated and terricolous under C. sativa trees.
Macromorphology of basidiomes, in particular because of the orange pileus and the pink-orange
adnate spines (Fig. 1a), indicated the collection of H. rufescens. Microscopical analyses showed
basidiospores with ellipsoid and subglobose shapes (Fig. 1d), while it is reported that H. rufescens
possess globose to subglobose basidiospores (Olariaga et al., 2012 and therein). In fact, phylogenetic
analysis collocated the sequence in the H. ibericum clade (Fig. 2).
   A year later (2020), in the same zone, we collected other Hydnum specimens, again morphologically
very similar to H. rufescens. These had caps up to 6 cm wide, at first convex then plane or depressed,
with deep orange tones of the pileus (Fig. 3a,b), often umbilicate, rather hygrophanous, with
hymenophore composed by long conical hydni (spines) not flattened and well delimited, clearly
adnate and not decurrent to the stipe, from whitish to pink-pale orange colours. Stipe was cylindrical,
both central and eccentric, with peach orange colours of a lighter tone with respect to that of the cap.
Contemporarily and colocalising (in some cases just one meter distanced from each other) with the
orange cap specimens, we found other small Hydnum specimens with whitish-cream, light-ochre
coloured pileus (diameter up to 3 cm) and well-separated conical spines not decurrent to the stipe,
with whitish colours (Fig. 1b,c). Cylindrical stipe, sometimes thinned at the base or short, had the
same tones of the cap. The genomic barcode of the orange specimens resulted to be H. ovoideisporum,
instead those of the whitish-cream light ochre sporocarps fitted well with H. ibericum, as the basidioma
collected in 2019 (Fig. 2).



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        Fig. 1 – Hydnum ibericum found in a chestnut forest of Monte Rocca Romana: a) basidioma coloured with
        orange tones (December 2019, TUF135543); b-c) light-ochre coloured specimens (late autumn 2020,
        TUF105918 and TUF105948); d) TUF135543 basidiospores; e) TUF135543 basidia.; f) TUF105918
        basidiospores. Bar = 10 µm.

Optical microscopy of the basidiospores of all Hydnum specimens (orange and light-ochre coloured
specimens) showed smooth ellipsoid basidiospores with granular content. Microscopic measurements
determined on basidiospores of H. ibericum fungi (Fig. 1f) gave means of 7.5 x 4.5 µm (n = 9) and
Qm = 1.7; [(9.8)-7.5-(6.2) x (4.9)-4.5-(3.7) µm, Q = (2.0)-1.7-(1.4)]. Clamp connections were visible
at the basis of basidia. Microscopic measurements determined on basidiospores of H. ovoideisporum
(Fig. 3c) revealed basidiospores with means of 7.6 x 5.6 µm (n = 9), Qm = 1.3; [(8.7)-7.6-(7.1) x (6.3)-
5.6-(5.0) µm, Q = (1.5)-1.3-(1.2)]. Basidia with two-four sterigmata (Fig. 3d). Clamp connections
were evident.

Entoloma sp. subgenus Cyanula
The small blue fungi found had a mycenoid appearance (Fig. 4a), small conical, campanulate caps,
afterward rather plane (up to about 1 cm). The pilei were translucently striate, showing bright blue
lines alternating with light blue-whitish stripes, turning darker when dried. The pilei were squamulose
in particular at the centre, with undulating margins. Adnate lamellae and lamellule, whitish with pale
blue tones, moderately crowded. The long lithe stipe (about 6 cm) was smooth or slightly tomentose,
azulene blue coloured and with white tomentum at the base.




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                        Fig. 2 – Phylogenetic tree upon alignment of the nuclear ribosomal ITS sequences of Hydnum species, identified by their UNITE/GenBank accession
                        number and associated species. The sequence KF879468 from Hydnum sp. was used as outgroup. The new samples described in the article are indicated
                        by asterisks and sequences from holotypes by hashes. Bayesian posterior probabilities >70% are reported on the main branches. The final mean standard
                        deviation of the separated frequencies was < 0.008.


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                   Fig. 3 – a-b) Hydnum ovoideisporum basidiomata found in late autumn 2020 on
                   the edge of a road crossing a chestnut forest; c-d) specimen TUF105919: basidia
                   and basidiospores. Bar = 10 µm.




                Fig. 4 – A specimen of the blue Entoloma sp. showing mycenoid appearance (a).
                Microscopic observations: (b) polygonal (5-7 angles) basidiospores with coloured lipid
                droplets; (c) lamella edge; (d) coloured cellular structures of the lamella and pileus
                edges. Bar = 10 μm.

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                                 Fig. 5 – Phylogenetic tree with posterior probability values >70% obtained through Bayesian inference based upon alignment of the
                                 nuclear ribosomal ITS sequences of Entoloma species, identified by their UNITE/GenBank accession number and associated species. The
                                 sequences JF908005 from Entocybe turbida and UDB015647 from Entocybe nitida were used to root the tree. The final mean standard
                                 deviation of the separated frequencies was < 0.008. The new sample is indicated by an asterisk. The insert in the red box shows a vertical
                                 enlargement of the clade containing the new sample with the two nearest sequences.

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Upon microscopic analyses of the specimens, evident polygonal shaped basidiospores (Fig. 4b)
directed their determination towards the genus Entoloma, tentatively as the species E. lepidissimum.
However, phylogenetic analysis (Fig. 5) arranged the ITS sequence within the Cyanula subgenus.
Microscopic observations of these Entoloma sp. specimens showed polygonal (5-7 angles) nodulose
basidiospores (Fig. 4b) with apiculum and pigmented lipid droplets inside, average size 10.7 x 7.2
µm [(12.2)-10.7-(8.6) x (8.9)-7.2-(5.8) µm, Qm = 1.5 (n = 10), Q = (1.1)-1.5-(1.9)]. Some intermixed
tufts of clavate cheilocystidia were present on lamella edges, pigments were visible inside the cellular
structures (Fig. 4c,d). Clamp connections were not detected.

Ramariopsis pulchella
Small specimens of Ramariopsis pulchella were identified from their coralloid morphology (Fig. 6),
basidiomata about 1 cm in height, smooth, with white feet and violet stems and filiform branches 2-3
times dichotomously separated and pale violet endings. The ends were simple or widened like short
teeth. A BLAST search on the ITS sequence yielded a most similar match (96%) to the sequence
KX812470 identified as Ramariopsis pulchella, collected in 2014 in the Guizhou Province in China.




                        Fig. 6 – Lilac Ramariopsis pulchella basidioma.

Discussion
This is the first record of H. ibericum fungi in the area of chestnut woods of Monte Rocca Romana
inside the Regional Natural Park of Bracciano-Martignano, in Latium and the first record for Italy.
The peculiar habitat formed by the particular volcanic soil and vegetation, together with the fact that
the areas inside the Park are rather preserved, allow the presence of uncommon or in some case rare
fungi (Ferretti et al., 2020). Hydnum ibericum grew scattered in the same environment in proximity,
in some cases about one meter apart, of other Hydnum specimens identified as H. ovoideisporum. The
sharing of the same habitat, which is relatively constant for several square meters of the area, suggests
that the two species possess similar ecology, such as common ectomycorrhizal partner trees and soil
requirement. Until a precise determination was obtained from DNA analysis and phylogeny of the
different specimens, it could be thought that they belonged to the same species with differences in
morphology due to variability.
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    The H. ibericum samples show variability in colours of the pileus, varying from an orange tone
to light ochre or whitish colours perhaps because the latter were young specimens. Also, the spines
showed variable colouration from whitish to pink-orange, such as in the first specimen we identified.
On the other hand, H. ovoideisporum was clearly characterised by an orange pileus as reported in the
original description by Olariaga et al. (2012).
    It is reported that the shape of the basidiospores is the most objective and constant character to
distinguish the species (Ostrow and Beenken, 2004; Olariaga et al., 2012). However, both H. ibericum
and H. ovoideisporum are described to possess ovoid to broadly ellipsoid basidiospores (Olariaga et
al., 2012; Vizzini et al., 2013; Niskanen et al., 2018). In fact, the morphological characteristics that
are constant in all the H. ibericum and H. ovoideisporum found in Monte Rocca Romana, were the
hymenophore adnate to the stipe and the ellipsoid shape of the basidiospores, particularly evident in
H. ibericum species. Probably the presence of these two species is underestimated in part because of
the macromorphological similarity with other Hydnums (such as H. rufescens, H. vesterholtii) and
also for the general lack of application of molecular barcoding to this genus.
    In the context of the presence of uncommon or rare fungi in the Park areas, the discovery of an
undetermined species of blue coloured Entoloma determined afterward in the clade Cyanula and of
the Ramariopsis pulchella draws interest.
    Genus Entoloma (Fr.) Kumm. contains more than 1,500 species, distributed in the most
heterogeneous habitats with respect to longitude, latitude and altitude. The taxonomy of Entoloma is
extremely difficult and still much in-progress with new species defined every year (He et al., 2020;
Noordeloos et al., 2021). Molecular phylogeny is essential for the taxonomy of this genus because
genetically different species do not always have significantly distinct morphological traits, on the
contrary differences in their morphology as for instance in colour can occur in the same species.
The high genetic variability of Entoloma (Vila et al., 2013) could lead to think that this genus has a
favourable adaptive ability to produce modifications to its DNA, for example through the crossing of
genetic sets coming from different but phylogenetically close Entoloma species.
    The blue colourations, the striate cap and the size of the spores could lead to the hypothesis that the
Entoloma specimens belong to E. pseudocoelestinum Arnolds (Arnolds, 1982) or to E. lepidissimum
(Svrček) Noordel. in incertae sedis (Morozova and Vila, 2014). Both species are rare (Noordeloos,
1982; Clericuzio and Vizzini 2011). Phylogenetic analyses led to exclude the last identification and
only collocates the Entoloma in the Cyanula subgenus. Noordeloos describes the Clade/group Cyanula
as including bluish species, all clampless, in the subgenus Leptonia but shown to be phylogenetically
rather distant from the Leptonia with clamp connections (Noordeloos et al., 2021). The two closest
species E. coelestinum (Fr.) Hesler voucher HMLD1659 giving rise to the KC257434 sequence and
the Entocybe nitida (Quél.) T.J. Baroni, Largent & V. Hofst. voucher TENN:067505 of the KU058498
sequence belong to groups that are genetically far from the position of the new sequence of Entoloma
sp. found on Monte Rocca Romana, in accordance with the fact that E. coelestinum is described to
have smaller spores and absent cheilocystidia (Morozova and Vila 2014) and the macroscopic and
microscopic characteristics described for Entocybe species (Baroni et al., 2011) differ from that of
our specimens. Anyhow, even these closest two relatives had a genetic distance of 69 (Accession
KC257434) and 61 (Accession KU058498) nucleotide and indel variations. Unfortunately, neither
specimen has been described morphologically. The E. gomerense Wölfel & Noordel. (Lorås et al.,
2014) has a quite similar morphological appearance and is part of the Cyanula clade, therefore it
would have been interesting to include its sequence in the DNA analysis however no sequence was
available in the public databases. Therefore, we were not able to attribute the specific name to the
Entoloma specimens from the Rocca Romana Forest.



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    Ramariopsis pulchella (Clavariaceae) is a globally distributed small coralloid species that can
easily go unnoticed but are considered rare. Originally named Clavaria pulchella by Boudier in 1887,
the species was transferred to Ramariopsis by Corner in 1950. Our observation of this fungus might
have an important significance from an ecological point of view. In Sweden R. pulchella is included
among the 130 fungal species, which have been designated as indicators or “signal species” of forests
stand worthy of conservation (Senn-Irlet, 2005). The fungus is reported to be rare by some European
studies (Arnolds et al., 1995; Shiryaev, 2009; Jindrich, 2012). In Italy a few specimens were described
in some Reserves or Protected Areas of Tuscany (Ricci and Perini, 2001; Salerni and Perini, 2007).
Because of its rarity in some European countries, it was also included in the list of species with threat
categories, as in the Red List of Macromycetes of the Czech Republic in the CR category - critically
endangered species. It is suggested that the reason for its rarity is mainly the loss of suitable habitats,
i.e. native areas with bare clay among herbs, as well as underwood between moss or leaves (Volobuev
and Bolshakov, 2017). Also in Switzerland the species is inserted in the ‘Lista Rossa delle specie
minacciate in Svizzera: macromiceti’ (Senn-Irlet et al., 2007) and ‘Lista delle specie prioritarie a
livello nazionale’ (UFAM, 2011).
    The territory included in the Bracciano-Martignano Regional Natural Park is recognised to
be characterised by a wealth of biodiversity with regards to flora and fauna, but also subjected to
significant threats. For example, one species of aquatic quillwort was discovered and found only
in the Bracciano Lake in 2013, Isoetes Sabatina Troìa & Azzella and is now critically endangered
(Magrini et al., 2020). Furthermore, in 2018 the presence of the rare Rosalia alpina L. beetle was
noted in the beech forest of Monte Raschio (Cerulli, 2019).
    The findings reported are indicative of the multiplicity of fungal species and the relevant
biodiversity of the habitats in the various volcanic areas included in the Bracciano-Martignano
Regional Natural Park, and confirm the importance of environmental preservation, also to allow the
continuous blooming of interesting fungi.

Acknowledgements
We would like to thank the Gruppo Micologico Fiorentino “P.A. Micheli”, its president Gianfranco
Di Cocco and Marco della Maggiora for the interesting and useful discussion about Hydnum species.
Irja Saar was supported by the Estonian Research Council (PRG1170), the European Regional
Development Fund (Centre of Excellence EcolChange).

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