10.6092/issn.2038-3460/17083
In the past decades, reproductive biomedicine has quickly developed and become widespread, producing a number of new options that have challenged the definition of kinship and parenthood, bodies and gender relations and even of nature and life itself. Reproductive biomedicine is embedded in the ongoing construction of our wider social imagination, producing a re-imagining of the “facts of life”. Here, we can see how biomedical knowledge fosters a reframing of material bodily tissues. The same biological material can assume a different ontological status according to the sociomaterial processes in which it is embedded. Exploring the process of bioobjectification of embryos in an Italian context, this introduction describes how the equation between embryos and human life itself emerges inside and outside of labs and illustrates how the biomedical conceptualization of embryos is strongly dominated by moral and ethical concerns.
application/pdf
2038-3460
17083
1
en
Manuela Perrotta
https://creativecommons.org/licenses/by-nc-sa/2.5/
Tecnoscienza – Italian Journal of Science & Technology Studies
human life itself
Creating Human Life Itself: The Emerging Meanings of Reproductive Cells among Science, State and Religion
Text.Serial.Journal
https://tecnoscienza.unibo.it/
4
Editorial
2013-07-12
2023-05-30
2013-07-12
2023-07-17
7-21
TS
Manuela Perrotta
2013/07/12
10.6092/issn.2038-3460/17083
7
Norwegian University of Science and Technology
2038-3460
1
human life itself
en
21
Creating Human Life Itself: The Emerging Meanings of Reproductive Cells among Science, State and Religion
https://tecnoscienza.unibo.it/article/download/17083/16003
4
                                                         Guest Editor’s Introduction

Creating Human Life Itself
The Emerging Meanings of Reproductive Cells among
Science, State and Religion
!
Manuela Perrotta
Norwegian University of Science and Technology
!
    Abstract In the past decades, reproductive biomedicine has quickly devel-
    oped and become widespread, producing a number of new options that have
    challenged the definition of kinship and parenthood, bodies and gender rela-
    tions and even of nature and life itself. Reproductive biomedicine is embed-
    ded in the ongoing construction of our wider social imagination, producing a
    re-imagining of the “facts of life”. Here, we can see how biomedical
    knowledge fosters a reframing of material bodily tissues. The same biological
    material can assume a different ontological status according to the socio-
    material processes in which it is embedded. Exploring the process of bio-
    objectification of embryos in an Italian context, this introduction describes
    how the equation between embryos and human life itself emerges inside and
    outside of labs and illustrates how the biomedical conceptualization of em-
    bryos is strongly dominated by moral and ethical concerns.

    Keywords: Assisted reproductive technologies, embryo, ontological shifting,
    bio-objectification, human life itself.

    Corresponding author: Manuela Perrotta, Department of Interdisciplinary
    Studies of Culture, Dragvoll, Edvard Bulls veg 1- 7491, Trondheim, Norway –
    Email: manuela.perrotta@aruba.it.



1. Introduction

    In August 2006 I participated in my first EASST (European Associa-
tion for the Study of Science and Technology) Conference in Lausanne,
Switzerland. The title of the conference was Reviewing Humanness: Bod-
ies, Technologies and Spaces, and I thought this would be the perfect place
to present a piece of my then ongoing PhD work in the field of Assisted
Reproductive Technologies (ART). It was one of my first presentations at
an international conference and my colleague Roberto Lusardi and I pre-
sented a joint paper on the construction of the body in ART centres and
intensive care units (Lusardi and Perrotta 2006). At the end of our
!
TECNOSCIENZA
Italian Journal of Science & Technology Studies
4(1) pp. 7-21 – ISSN 2038-3460
www.tecnoscienza.net                              2013
8                                                             Tecnoscienza - 4 (1)

presentation, a feminist British scholar (this is how she introduced her-
self) asked me: “You have talked a lot about this embryo, Why do you call
it embryo? Are you a Vaticanist? I see just a clump of cells”. Aside from
the shock, I answered that it was a fieldwork-related effect, since all of the
professionals and patients I met referred to “embryos” in their narratives.
However, this question pointed out what I had taken for granted in my
research. I want to thank this (unknown) colleague, because her comment
fostered my reflection on what an embryo is and how its meanings are
relatively stabilized both inside and outside of clinics. In fact, although
during my fieldwork I became familiar with the biological nomenclature –
which refers to zygotes, blastomeres, morulae, blastocysts and so on –
spending time with biologists in reproductive labs I also absorbed what in
the Italian context is taken for granted, and is indeed quite widespread
around the globe: an embryo is a fertilized egg after cellular division (even
though it is almost never specified in ART discourse when an embryo
stops being an embryo and becomes something else, like a foetus). More-
over and more relevantly for this special issue, “the embryo” is conceptu-
alized as the first stage of human life, and this notion is so stabilized in
some contexts – such as the Italian one and others – that it had become
unchallenged and even unquestioned in Italian public debate.
    Taking an STS perspective, this special issue aims at challenging this
conceptualization of embryos as well as the emerging meanings of repro-
ductive cells, using the Italian case as a prime example for the study of
how a bio-object emerges from a network of heterogeneous elements and
becomes relatively stable.
    In the introduction to a recent edited book evocatively titled Bio-
Objects: Life in the 21st Century (Vermeulen et al. 2012), Andrew Web-
ster defines the bio-object as “a useful conceptual device or heuristic to
refer to socio-technical phenomena where we see a new mixture of rela-
tions to life or to which ‘life’ is attributed” (Webster 2012, 6). Through
the bio-objectification process, novel socio-technical – including political
– relations are made possible. At the same time, the possible assemblages
can be more or less robust and different bio-objects are more or less able
to take their shape through time and space.
    To put this in Actor-Network Theory (ANT) terms, studying the bio-
objectification process means studying how the durable orderings are
achieved, how bio-objects become such, how they are put in place and
stay that way and how change comes about. On the other hand, following
the so-called ecological approach (Star and Griesemer 1989; Fujimura
1995), what is interesting in this process is the construction of hybrid ar-
rangements in which bio-objects move, being constantly renegotiated
through an ecology of actions. Susan Leigh Star and James Griesemer, in
their seminal article published in 1989, propose to take into account the
coexistence of complex “multiple translations”. They stress the collective
work of coordination, alignment mechanisms and translation chains be-
tween the different actors and worlds involved. To study the embryo as a
Perrotta                                                                          9!
bio-object, therefore, we should be able to describe its translations by dif-
ferent actors, including ART professionals, patients, politicians, scientists,
bio-ethicists and social movements (supporting patient rights or moral
and religious instances). Moreover, to explore the multiple translations
we need to follow the intricacies of actors’ relationships and pay greater
attention to powerless actors or “dissidents” within the enrolled actors
(Star 1991). The Italian case illustrates how the construction of the bio-
object embryo, with its current shape, produces a demarcation between
those interested in keeping embryos as an abstract model of human life in
a nutshell, and those who see embryos as future possibilities and hope,
both for reproductive purposes and biomedical research.
    The Italian embryo is an excellent example of how a bio-object is
made. How does the special ontological status of human embryos come
about? How is this ontological status constructed inside and outside of
reproductive laboratories? How is the idea of human life itself “created”?
    To answer these questions, the core idea is to explore what Charis
Thompson (formerly Charis Cussins) defined in ontological choreography
(Cussins 1996; Thompson 2005). With the term “ontological choreogra-
phy”, Thompson refers to:

               The dynamic coordination of the technical, scientific, kinship,
           gender, emotional, legal, political, and financial aspects of ART
           clinics. What might appear as an undifferentiated hybrid mess is
           actually a deftly balanced coming together of things that are gen-
           erally considered parts of different ontological orders (part of na-
           ture, part of the self, part of society). These elements have to be
           coordinated in highly staged ways so as to get on with the task at
           hand: producing parents, children, and everything that is needed
           for their recognition as such (Thompson 2005, 8).

    Therefore, the notion of ontological choreography aims to stress the
dramatic ongoing change in the ontological statute of human gametes and
embryos, which in ART centres changes from potential person to element
of the reproduction of a person and, eventually, to a non-person. An em-
bryo, for instance, can be a potential person when it is part of the treat-
ment, or a suspended animation when it is frozen, or a biological source
selected for research purposes, or biological material of poor quality
when it is unsuitable to any of the available purposes. Therefore, ART
practices become loci of achievement, multiplication and coordination of
ontological variations.
    Here the focus is not on the choreography (i.e., the coordinated action
of ontologically heterogeneous elements), but on the ontological shifting:
how multiple translations generate a network able to crystallize biological
substances into a specific shape with a specific array of meanings, produc-
ing bio-objects. The empirical case under analysis in this special issue is
the current changes in the ontological status of cells in Italian reproduc-
tive biomedicine and beyond. In other words, how did a clump of cells
10                                                          Tecnoscienza - 4 (1)

become the beginning of human life and even a proto-person?
    Answering this question will provide a twofold opportunity. From an
empirical point of view, it will allow exploration and explanation of many
paradoxes that characterize the Italian case, where embryos have more
rights (or at least, they had for a while, as we will see) than foetuses and
even mothers; where some practices are illegal in the country (such as
gamete donation) but people who go abroad to undergo the same prac-
tices are not blamed; where the State openly discriminates against some of
its citizens, such as singles or homosexual couples (who are not allowed
to undergo treatments in Italy), but people are still willing to cross the
border for the benefit of treatments that are illegal in their own country
(if they can afford to go abroad); where surplus embryos cannot be do-
nated to research or other patients for reproductive purposes, or be de-
stroyed (since embryos are considered people at the cellular stage), but
they can be “abandoned” and kept frozen; where the State forbids re-
search on national embryos, while allowing scientists to import foreign
stem cell lines.
    This special issue aims at exploring the bio-objectification of repro-
ductive cells in an Italian context, from the perspectives of a multitude of
actors. The material and regulatory aspects of life have already been un-
der investigation in STS (Hauskeller et al. 2005; Waldby and Mitchell
2006; Sunder Rajan 2006; Franklin 2007; Cooper 2008 – to mention just a
few examples of this strand of research), and this special issue represents
an interdisciplinary enrichment of the STS literature on the study of the
relationship between life and technoscience.
    The first contribution is a lecture by Sarah Franklin on In Vitro Ferti-
lization (IVF) as a visual culture. The conversion of the human embryo
into both a tool and a lens allows the author to revisit a series of broad
sociological questions concerning technology, reproduction, genealogy
and the future of biological control.
    The Essays section offers four contributions. The article by Patrick
Hanafin, exploring the relationship between biopolitics, law and repro-
ductive citizenship, proposes an interesting excursus of the Italian regula-
tory path on ART after the establishment of the Italian law in 2004, focus-
ing on how different individuals and groups challenged the regulation in
local, national and international courts. Alessandra Gribaldo’s article fo-
cuses on the concept of “micro-reproduction”, on the relevance of visual
tools in this domain and the processes of constant re-signification that in-
volve professionals as well as prospective parents. The contribution by
Giulia Zanini illustrates the variable meaning of donor embryos for Ital-
ian cross-border reproductive travellers approaching donor conception,
analysing how they make sense of different embryos’ trajectories. The ar-
ticle by Lorenzo Beltrame discusses the cultural meaning and political us-
es of ethical stem cells in Italy, following the embryo and its conceptual-
ization in the biomedical research domain.
    The Conversations section presents two contributions that pave the
Perrotta                                                                       11!
way for the debate on the ART-stem cell research interface; the first con-
tains three comments by Laura Lucia Parolin, Ingrid Metzler and Alex-
ander Schuster on the book Fecondazione E(s)terologa by Carlo Flamigni
and Andrea Borini; the second collects two interviews with top Italian
scientists in the field of stem cell research, Elena Cattaneo and Giuseppe
Testa, introduced by a reflection on the Italian pathways of stem cells by
Assunta Viteritti.
    In the following pages I shall introduce the main issues converging in
this special issue, and try to provide a framework that makes the Italian
case more understandable to an international public. First, I shall intro-
duce the Italian national regulation on ART and the moral monopoly im-
posed by the Catholic front. Although the attempt to create a new moral
order in the country failed, it created a gap between politics and lay peo-
ple, and moral arguments and daily life. Secondly, I shall discuss the pro-
cess of bio-objectification of embryos that causes in vitro embryo to be-
come embryo as the beginning of life and embryo as a proto-person. Finally,
I will discuss the construction of “the embryo” as an abstract representa-
tion of (human) Life Itself (Franklin 2000). Regarding word use, I do not
use the word “creation” to refer to the production of embryos in ART
labs, but to the sociomaterial construction of the equivalence between
embryos and human life itself, both inside and outside of labs.


2. The Moral Monopoly and the Italian Regulation on ART
    The introduction of the law on ART in 20041 was the result of a long
negotiation process carried out by numerous political and social actors,
which deployed different rationalities and resources to support diverse
proposals for change. The debate on the ethical aspects of ART was ex-
tremely heated, with radically opposed moral and religious stances being
taken. On the one hand, the Catholic front – highly influential in the Ital-
ian debate – proposed that restrictions should be imposed on therapeutic
treatments by virtue of the (moral) argument that an embryo is not only a
life-form but also a future person. On the other hand, there were those
who argued that it was necessary to go beyond biological limits in order
to adjust reproductive capacity to the life-choices typical of contemporary
society.
    The position of the Catholic Church was expressed in 1995 by John
Paul II in the evangelical letter “Evangelium Vitae”, in which the Pope
argued that biomedical technologies (here referring to the manipulation
of gametes in the lab) go well beyond a reasonable dominion over nature.
The main criticism was directed at non-therapeutic research on human
embryos and their destruction or cryopreservation, the selection of char-
!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
1
 Law No. 40, February 19, 2004, Regulations relating to medically assisted procre-
ation published in the Official Gazette No. 45, February 24, 2004.
12                                                                   Tecnoscienza - 4 (1)

acteristics or gender of the newborn, heterologous fertilization and all
cases of surrogate motherhood.
    After a long line of attempts, Law 40 forbade surrogate motherhood
of any kind, the insemination of homosexual couples and singles, insemi-
nation after the partner’s death and of women in non-precocious meno-
pause. Moreover, Law 40 increased penalties for all violations2 and intro-
duced “new” restrictions in addition to those already present: heterolo-
gous fertilization (i.e., with gametes from donors external to the couple),
the production of more than three embryos per cycle, the cryoconserva-
tion of embryos, the pre-implantation diagnosis (for a discussion on the
limitations operating in Italy before Law 40 see Parolin and Perrotta
2012). Moreover, the law ordered that all embryos produced (no more
than three) must be transferred to the uterus in any circumstances. What
is peculiar is that the law gave embryos rights that not even a foetus has,
since Law 194 of 1978 on abortion has not been overturned or modified
to create further restrictions on abortion (for a discussion on the differ-
ences among the debate on ART and that on abortion see Metzler in this
issue).
    Furthermore, by equating the embryo with a person, Law 40 made its
rights prevail over those of the mother. The law was widely discussed and
criticised in regard to both its ethical and clinical aspects. Scientific pro-
cedures were paralysed, while the clandestine market and the search for
assisted reproduction in other more permissive countries were stimulated.
A broad political movement developed around these criticisms and mobi-
lized public opinion for some months, leading to a referendum to abro-
gate the law. However, the referendum was not successful, because a
quorum (50% plus one of those entitled to vote) was not reached (just
25.3% voted).
    The bioethical position expressed by the law accommodates the con-
servative and patriarchal models of gender and familial relations, creating
a moral monopoly that impedes a pluralist development of multi-ethical
frameworks. Defining the rights of embryos as those of proto-citizens was
in fact meant to establish a monolithic view of bio-ethical issues (Hanafin
2007). However, this does not seem to correspond to the uses and cus-
toms in place. Referring to the year 2010, the annual report3 of the Health
Ministry to the parliament argues that 69,797 couples have undergone In
Vitro Fertilization (IVF) or IntraCytoplasmic Sperm Injection (ICSI)
treatments, and through these techniques 15,274 pregnancies were ob-

!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
2
  The law stated that breaches of its provisions were to be punished with impris-
onment for up to 6 years and a fine of up to €150,000 for operators, as well as
immediate closure of the center.
3
  Relazione del Ministero della Salute al Parlamento Italiano sullo stato di attuazione
della legge contenente norme in materia di procreazione medicalmente assistita
(Legge 19 Febbraio 2004, n. 40, articolo 15) – Anno 2010 – issued by the Ministry
of Health on 12 June 2012.
Perrotta                                                                   13!
tained. Moreover, the limitations imposed by the law have generated re-
productive tourism to countries with more liberal regimes.
    A recent study by the European Society for Human Reproduction
(Shenfield et al. 2010) claims that Italians are first in the world for pro-
creative tourism (31.8% of all foreign patients) and that the main reason
why couples are turning to centres abroad is the national law (70.6% of
cases among Italian patients). Some authors refer to this phenomenon as
“cross-border reproductive care” (Inhorn and Gürtin 2011) to avoid the
negative connotation related to the word “tourism”, and emphasize that
there is an element of “forced necessity”. Interestingly, cross-border re-
productive care in Italy is not socially stigmatized in the public debate. It
saw considerable development in recent years and gave cross-border pa-
tients many new choices regarding conception practices, disclosing how
prospective parents understand reproductive cells and embryos as ele-
ments of their parental project (see Zanini in this issue).
    Finally, several couples, often supported by fertility centres and asso-
ciations, appealed to local courts, to the Italian Constitutional Court and,
most recently, to the Court of First Instance of the European Court of
Human Rights in Strasbourg to modify some of the restrictions (for a de-
tailed discussion on the judicial interventions see Hanafin in this issue).
Many of the limitations imposed by the law lasted till 2009, when the Ital-
ian Constitutional Court declared the law partially unconstitutional. Be-
yond surrogacy, which is not even on the public agenda, two main prohi-
bitions still remain in force: heterologous insemination and access to the
treatments, which is still not permitted to homosexual couples and sin-
gles. On the one hand, the lack of willingness of the main political parties
(both the right and left wings) to revise the law is due to the fact that this
is still a highly sensitive issue for Catholics. On the other hand, the heter-
ogeneous alliances that crystallized the embryo as a proto-citizen are
aligned with the dominant narrative of a conservative Catholic-oriented
morality and ethics, which remove, cover and annihilate all antagonistic
narratives, fostering a monolithic view of bioethical issues.


3. What is an Embryo? Embryos-in-the-making

    Sarah Franklin and Celia Roberts (2003) have shown how the embryo
represents a social material actor playing within a set of technologies and
social practices, and how it is setup through these practices (like making a
picture of the embryo, or selecting, transferring and freezing it). The em-
bryo’s social life, as it is called in the scholars’ wording, becomes im-
portant as well as its life in the public and political debate (Mulkay 1997).
    Referring to the work of Donna Haraway, Sarah Franklin remarkably
notes that the embryo is a cyborg:
14                                                                Tecnoscienza - 4 (1)

          [It] is as much a product of high-tech procedures, such as ovula-
       tion induction and cryopreservation, as it is of hope for a child and
       the desire for technological assistance to overcome infertility.
       (Franklin 2006, 173)

    The embryo is at the same time a product of biomedical knowledge,
lab procedures and patients’ alignment to the treatment, as well as the
hope for a child, an essential element of the parental project, and the ma-
terial production of the desire for parenthood. As many other kinds of
cells, in vitro embryos have a limited “life” in culture. The extracorporeal
embryos cannot reach the foetal stage outside the womb. They could not
exist as we know them without visualization devices (Franklin in this is-
sue) that make them available to professionals (Perrotta 2008a), patients
(Gribaldo in this issue) and the lay public (Lie 2012). As Merete Lie
(2012) has shown, these images represent reproductive cells as autono-
mous and independent entities. Similar to what happens when the foetus
is detached from the womb and presented as an individual, embryos have
an appearance detached from the body, seemingly independent of it.
    What I want to claim here is that the embryo as a bio-object, emerging
from multiple translations, is multiple itself: abstract and concrete, mate-
rial and conceptual, general and specific. However, conceptualizing the
embryo as an immaterial entity or as the product of biomedical technolo-
gies, reproductive knowledge and (unpredictable) bodies results in ex-
tremely different consequences. For instance, a regulation built on a gen-
eral, conceptual and abstract embryo fails to take into account the com-
plex heterogeneous network from which material, specific and concrete
embryos emerge. Wombs, bodies and their reactions to hormonal treat-
ments, as well as patients’ lives, affective relationships and their ability to
match the treatment schedules disappear in front of a purified idea of an
embryo.
    The autonomous life of the embryo is built in reproductive centres as
well as in other loci. ART centres enable infertile couples to achieve their
goal by means of technology. But while the couples’ objective is pregnan-
cy (‘the babe in arms’), the technoscientific objective becomes the pro-
duction of the embryo (for a detailed discussion see Perrotta 2008b).
More precisely, the production of a reasonable number of embryos (or
the three permitted by law between 2004 and 2009), if possible belonging
to the best class (according to a classification scale in use), or at least ones
which are implantable or cryoconservable. When the process comes to an
end (positively) and the embryos have been produced, they are trans-
ferred to the “natural” site of reproduction, i.e., the womb. The embryos’
reintroduction into the patient’s uterus mark the completion of the organ-
izational process, although two weeks later might yield to a completely
different result from the patients’ point of view (a pregnancy or a failure).
    However, in the need to manage the pressure of high rates of failure,
the ART centres frame their success as their ability to “give embryos back
Perrotta                                                                  15!
to patients”. As I have argued elsewhere (Perrotta 2008b), the transfer is
a key stage in the reproduction process in the sense that it is the moment
when responsibility for a successful outcome passes from the ART centre
to the patient. If there are embryos (and usually there are some), the cen-
tre has done its duty and the team must wait and see if the patient’s body
will let the embryos implant themselves. Responsibility is not explicitly
attributed, but it is clear that everything “technically” possible has been
done, and now “nature” must take its course.
    To “give embryos back to patients” is not accomplished solely with
embryo-transfer; embryos are showed on monitors, their pictures are of-
ten attached to the clinical records and even commented on by the per-
sonnel with utterances such as “look at your little embryos!” (Perrotta
2008a). As Franklin argues in her lecture, witnessing a live human embryo
is “special”. These images merge highly specialized scientific imaging ap-
paratus with intimate human biological substance. Moreover, as Gribaldo
and Zanini show in this issue, the possibility of watching one’s own em-
bryos, in addition to the patients’ expectations, produces different under-
standings of embryos themselves, according to the configurations of ele-
ments in which they are embedded. Anyhow, for reasons related to the
organizational work of ART centres, in several circumstances the lab is
one of the loci where the ontological shifting from the in vitro embryo to
the embryo as the beginning of human life is accomplished.


3.1. The Personification of Embryos

    With the introduction of Law 40 and its prohibition on freezing em-
bryos (that lasted until 2009), it was necessary to take action to resolve
some ethically controversial issues: what was to be done with the embryos
produced before the law, which every centre kept in storage? How could
the principle of equating the embryo with a person, and therefore guaran-
teeing it the same rights, be applied retrospectively to the already-existing
embryos?
    When Law 40 was enacted, in Italy there were around 30,000 cry-
oconserved embryos stored in several centres spread around the country.
Some of these embryos were supposed to be used by aspiring parents in
further attempts to obtain a pregnancy. But what would have happened
to those belonging to couples who had already had children and did not
want any more? Or in cases where the couple did not intend to procreate
further (because of separation, adoption, age)? In this case, the law states
that the embryos produced and frozen before its enactment must be kept
in their actual state at the expense of the couple, or they must be declared
“abandoned” or “neglected” (in stato di abbandono is the expression used
in the law). Couples were therefore required to pay for the maintenance
of their own embryos (the costs relative to the cryoconservation of em-
bryos varied from centre to centre, but they range between 500 and 1000
16                                                            Tecnoscienza - 4 (1)

Euros a year), or sign an abandonment form with which they lose all
rights over their embryos. Law 40 required the establishment of a nation-
al embryo bank, which has never been created (for an updated discussion
of the failure of the National Biobank, see Zanini, this issue). Instructions
have not yet been issued on what is to be done with abandoned embryos,
which are currently awaiting conservation in what are now often called
“orphan embryo banks”. What is certain is that other options – such as
donation to other couples or for research purposes, or disposal, which
represented 77% of choices before the enactment of the law (Cattoli et al.
2005) – are still illegal.
    Although expressions like “orphan embryos”, “abandoned embryos”
and “adoption of embryos” recall the principle of the law which equates
them with people, these definitions reproduce and embed the ontological
shift from the in vitro embryo to the embryo as a proto-person. On the one
hand, this is an expression of the moral monopoly I have presented in one
of the previous sections. On the other, this understanding of embryos is
problematic and morally controversial, especially in relation to the effects
it produces on the ART stem cell research interface. Even though embry-
os produced before or after the introduction of the law cannot be used
for research purposes, Law 40 (nor other laws) does not forbid stem cell
research in the country. Nevertheless, the prohibition on embryo research
envisaged by Law 40 is not a legal preclusion of research on embryonic
cell lines created in other countries – also because EU legislation stipu-
lates the free circulation of goods and therefore makes it impossible to
prohibit the importing of stem cells. Therefore, Italian scientists are al-
lowed to do stem cell research in Italy, as long as they are not “killing Ital-
ian embryos” (Melzner 2011).
    Since the ambiguity of banning research on embryos by virtue of their
alleged sacredness (for a discussion on the tension between the sacred
and the profane that characterizes embryos, see Thompson 2005), the sci-
entists who work with human embryonic stem cells (hESCs) are consid-
ered “unethical”, and are tolerated but not publicly supported. As the
interviews with Elena Cattaneo and Giuseppe Testa in this issue illustrate,
the Italian public debate reflects this ambiguity reproducing an ethical
contrast between the research on adult stem cells (especially iPS – In-
duced Pluripotent Stem Cells) and embryonic stem cells.


3.2. Embryo, or Non-embryo: That is the Question

    The process of bio-objectification of an embryo as a proto-person fos-
tered the proliferation of forms of non-embryo. In his contribution to this
special issue, Lorenzo Beltrame, analysing the debate on “ethical stem
cells”, presents an interesting analysis of the advent of several forms of
quasi-embryos, deployed to circumvent the Italian regulatory regime and
allow research on stem cells. Another case of non-embryos as a residual
Perrotta                                                                   17!
category created to sidestep the meanings attached to the notion of em-
bryos has been the creation of pre-embryos, symbolic in nature and lin-
guistically justified in opposition to the law, which had the distinctive fea-
ture of not being subject to the same restrictions imposed by the law.
     In the months before the enactment of Law 40, the Italian public de-
bate turned to the issue of the “beginning of life” as the new main ethical
and moral issue. The debate, moreover, was framed as a controversy
promoting two adverse factions: the so-called “pro-life” movement
(summarized by the slogan “the embryo is one of us”) and those who did
not accept the classification of an embryo as a person and wanted to put
the rights of the mother before those of the unborn child.
     These positions brought different legal and technical decisions, par-
ticularly regarding the possibility of freezing surplus embryos: the first
group clearly rejected embryo cryopreservation in order to defend human
life; on the contrary, the second group was in favour of embryo freezing,
seeing it as a valuable tool to limit the negative effects of repeated hor-
monal treatments and surgery for oocyte collection. As we have seen, the
position that prevailed in Italy (until the intervention of the Constitution-
al Court in 2009) was that of the pro-life movement.
     In the first months after the enactment of the law, to avoid these re-
strictions operators of the reproduction centres, and their professional
associations, questioned the term embryo used in the law: when can a fer-
tilized oocyte be considered an embryo, therefore constituting the begin-
ning of human life and a person? Some scientists proposed a technical
distinction between embryo and pre-embryo on the basis of a medical in-
terpretation of the law, which enabled them to circumvent its provisions.
According to this interpretation, as a zygote the embryo should be a cell
with a single nucleus (of 46 chromosomes) in which the fusion between
the male and female gametes had already taken place, usually 36 hours
after the moment when the oocyte and the spermatozoon come into con-
tact. Otherwise, an oocyte fertilized less than 36 hours previously should
be considered a “pre-embryo” (or “pre-zygote”).
     The strategy of differentiation between embryo and pre-embryo – in
use in countries like Germany and Switzerland – had a technical basis:
pre-embryos had almost the same rate of survival as embryos, while freez-
ing oocytes (the only other option available to avoid wasting biological
material from female patients in each treatment) at the time had a very
low rate of survival after thawing. However, the notion of pre-embryos
assumed fundamental significance in the Italian bioethical debate on the
question of life, and the various stages of embryo development have been
used in the public debate to “establish” when life begins.
     The overlapping of biomedical and moral/ethical meaning produced
the failure of this strategy (for a deeper discussion see Perrotta 2011). The
pre-embryo became an example of how even in the bio-medical commu-
nity the moral and ethical categories were dominant in the reproductive
discourse. The bio-medical community was not rejecting the equation be-
18                                                           Tecnoscienza - 4 (1)

tween embryo and human life itself, but trying to negotiate the post-
ponement of the beginning of human life. This turn to the language of
biology (that in other circumstances would be viewed as “the natural”
language of ART) has been considered as a manipulatory attempt. The
term embryo, in fact, was (and still is) widely used inside and outside of
the scientific community, because it is considered a simpler and more
comprehensible language in order to communicate with personnel exter-
nal to the laboratory (doctors, nurses, anaesthetists, and so on) and with
patients. The generic expressions “fertilized oocyte” and “embryo” are
often used as commonplace terms, while biological nomenclature (zygote,
blastomere, morula, blastocyst and so on) is often set aside in ART cen-
tres.
    The attempt to negotiate the limitation imposed upon the medical
practice by redefining the category of embryo was subsequently blocked
by ministerial circulars and guidelines establishing that, although Italian
law made reference only to embryos, the restrictions also applied to pre-
embryos, since it had clearly been the intention of the legislators to pro-
tect potential “life” from its beginning: namely, the moment of encounter
between oocyte and spermatozoon, seen as the beginning of an unstop-
pable process that would lead to the birth of a child.


4. Conclusions: Creating Human Life Itself

    Through this introduction I have aimed at illustrating the process of
bio-objectification of embryos, looking at the ecology of actions through
which embryos are moving and which constantly renegotiate their mean-
ings. These multiple translations, for partially planned but also fortuitous
reasons, produce ontological shifts, crystallizing biological substances (the
in vivo embryo) into a specific shape (the embryo as the beginning of life
and as a proto-person) with a specific array of possible meanings (the pro-
tection of embryos, the donation of embryos for reproductive purposes,
the sacrifice of embryos for research, to mention just a few). Sarah Frank-
lin (2000) explored the emergence of the concept of “life itself”. Here, I
tried to illustrate how ART in the Italian setting has taken an active role
in the sociomaterial construction of embryo as human life itself. The ex-
cursus of examples I have presented in the last few pages had the purpose
of exploring how the ontological shift from in vivo embryo to embryo as
human life itself emerged from the entanglement of State, Science and Re-
ligion, in which ART is embedded.
    The Italian regulation of ART, whose main aim seems to be protecting
the embryo, can be read as the effect of the alignment of powerful actors
like Catholic hierarchies and the Conservative front, which built a moral
monopoly around these issues. I proposed here a framework that is able
to take into account a more complex ecology of actions, including those
of dissident actors – who actively participate in the different ontological
Perrotta                                                                       19!
shifts. My purpose was to show how the myth of the “creation of life in
the lab”, which is based on the equation between embryos and human life
itself, is partly supported in the ART centres for organizational reasons.
The production of embryos in the labs, their conceptualization as the end
of treatment and the beginning of a possible pregnancy, which translate
the presence of embryos into the core element of hope, participate in the
socio-material construction of the equivalence between embryos and hu-
man life. Moreover, I have analysed how the medical conceptualization of
embryos is highly dominated by moral and ethical categories, even when
the equivalence between embryos and life is discursively denied. The use
of categories of non-embryos to sidestep the moral status of embryos un-
intentionally and unwittingly reinforces the overlap between embryos and
human life itself.
    The contributions to this special issue further articulate this overlap.



Acknowledgments
    The idea for this Special Issue on the emerging meanings of reproductive cells
arose during my participation in the project Inside out: New images and imagina-
tions of the body, funded by the Norwegian Research Council through the Cultur-
al Values Programme (KULVER) 2010-2014. I would like to thank Merete Lie,
the project leader, for her continuous support, and my colleague Kristin Spilker
for her intellectual insights provided during the project.
    I am grateful to the reviewers for their critical and detailed feedback, and I
thank all the authors for their patience and enthusiasm while working under tight
deadlines. I wish to acknowledge the help provided by the Editorial Board of
Tecnoscienza. In particular, I want to thank Paolo Magaudda, Assunta Viteritti
and Attila Bruni for their moral support, and Stefano Crabu for his fruitful com-
ments on a previous draft of this introduction.
    Last but not least, I would like to express my great appreciation to Sarah
Franklin and the Cambridge Interdisciplinary Reproductive Forum for the inspira-
tion provided during my research visit to the University of Cambridge in Lent
2013, where I wrote this introduction.



References
Cattoli, M., Borini, A., Dal Prato, L., Bonu, M.A., Patuelli, M. and Flamigni, C.
   (2005) Stored Frozen Embryos: the Influence of Italian IVF Law, in “Human
   Reproduction”, 20 (suppl. 1), pp. 48i.
Cooper, M. (2008) Life as Surplus: Biotechnology and Capitalism in the Neoliberal
   Era, Washington, Washington University Press.
Cussins, C. (1996) Ontological Choreography: Agency through Objectification in
   Infertility Clinic, in “Social Studies of Science”, 26 (3), pp. 575-610.
20                                                                  Tecnoscienza - 4 (1)

Franklin, S. (2000) Life Itself: Global Nature and the Genetic Imaginary, in S.
   Franklin, C. Lury, and J. Stacey (eds.), Global Nature, Global Culture, Lon-
   don, Sage, pp. 188-227.
Franklin S. (2006) The Cyborg Embryo: Our Path to Transbiology, in “Theory,
   Culture & Society”, 23 (7–8), pp. 167-187.
Franklin S. (2007) Dolly Mixtures: the Remaking of Genealogy, Durham, NC,
   Duke University Press.
Fujimura, J. (1995) Ecologies of Actions: Recombining Genes, Molecularizing Can-
    cer, and Transforming Biology, in S.L. Star (ed.) Ecologies of Knowledge: Work
    and Politics in Science and Technology, New York, State University of New
    York Press.
Franklin, S. and Roberts, C. (2003) Innovative Health Technologies: The Social
   Life of the Embryo, published by the Department of Sociology, Lancaster
   University, Lancaster LA1 4YN, UK, at http://www.comp.lancs.ac.uk/-
   sociology/papers/Franklin-Roberts-Social-Life-of-Embryo.pdf
Hanafin, P. (2007) Conceiving Life. Reproductive Politics and the Law in Contem-
   porary Italy, Burlington, VT, Ashgate Publishing Company.
Hauskeller, C., Bender, W. and Manzei, A. (2005) Crossing Borders, Münster,
   Agenda Verlag.
Inhorn, M.C. and Gürtin, Z.B. (2011) Cross-Border Reproductive Care: A Future
   Research Agenda, in “Reproductive Biomedicine Online”, 23 (5), p. 665-676.
Lie, M. (2012) Reproductive Images: The Autonomous Cell, in “Science as Cul-
    ture”, pp. 475-496.
Lusardi R. and Perrotta M. (2006) The construction of body between life and
   death, paper presented at the EASST Conference 2006: Reviewing Human-
   ness: Bodies, Technologies and Spaces, August 23-26, Lausanne, Switzerland.
Mulkay, M. (1997) The Embryo Research Debate. Science and the Politics of Re-
   production, Cambridge, Cambridge University Press.
Metzler, I. (2011) Bewteen Church and State: Stemn Cells, Embryos, and Citizens
   in Italian Politics, in Jasanoff S. (ed.) Reframing Rights: Bioconstitutionalism in
   the Genetic Age, Cambridge, MA, MIT Press, pp. 105-124.
Parolin, L.L. and Perrotta, M. (2012) On the Fringe of Parenthood: Othering and
   Otherness in the Italian Assisted Kinship, in “About Gender. International
   Journal of Gender Studies”, 1 (2), pp. 100-131.
Perrotta, M. (2008a) Ri-produrre la vita: scelta tecnologica e visione professionale,
   in S. Gherardi (ed.), Apprendimento tecnologico e tecnologie di apprendimento,
   Bologna, Il Mulino, pp. 241-272.
Perrotta, M. (2008b) The Organizational Construction of the Body in the Assisted
   Reproductive Technologies, in F. Molfino and F. Zucco (eds.), Women on Bio-
   technology. Creating Interfaces, Milano, Springer, pp. 171-181.
Perrotta                                                                          21!
Perrotta, M. (2011) Il pre-embrione (non) è uno di noi: breve storia di una innova-
   zione inter-organizzativa tra istituzioni, comunità professionali e tecnologie, in
   “Sociologia del Lavoro”, 122, pp. 194-206.
Shenfield, F., de Mouzon, J., Pennings, G., Ferraretti, A.P., Nyboe A.A., de
   Wert, G. and Goossens, V. – ESHRE Taskforce on Cross Border Reproduc-
   tive Care (2010), Cross border reproductive care in six European countries, in
   “Human Reproduction”, 25 (6), pp. 1361-1368.
Star, S.L. (1991) Power, Technologies and the Phenomenology of Standards: On
    Being Allergic to Onions, in J. Law (ed.) Power, Action and Belief: A New So-
    ciology of Knowledge? London, Routledge & Kegan Paul, pp. 26-56.
Star, S.L. and Griesemer J.R. (1989) Institutional Ecology, “Translations”, and Co-
    herence: Amateurs and Professionals in Berkeley’s Museum of Vertebrate Zool-
    ogy, 1907- 1939, in “Social Studies of Science”, 19, pp. 387-420.
Sunder Rajan, K. (2006) Biocapital: The Constitution of Postgenomic Life,
   Durham, NC, Duke University Press.
Thompson, C. (2005) Making Parents: The Ontological Choreography of Reproduc-
   tive Technologies, Cambridge, MA, MIT Press.
Vermeulen, N., Tamminen, S. and Webster, A. (eds.) (2013) Bio-Objects. Life in
   the 21st Century, Farnham, Ashgate.
Waldby, C. and Mitchell, R. (2006) Tissue Economies: Blood, Organs and Cell
   Lines in Late Capitalism, Durham, NC, Duke University Press.
Webster, A. (2012), Introduction: Bio-Objects: Exploring the Boundaries of Life, in
  N. Vermeulen, S. Tamminen and A. Webster (eds.), Bio-Objects. Life in the
  21st Century, Farnham, Ashgate, pp. 1-10.
