how many atp does glycolysis produce
- Reactions 6 and 9 are coupled with the formation of ATP. To be exact, 2 ATP are produced at step 6 (remember that the reaction occurs twice) and 2 more ATP are produced at Step 9. The net production of visible ATP is: 4 ATP. How many ATP and NADH are produced from Glycolysis? Confidence votes 106. it produces 4 ATP molecules but then uses 2 ATP molecules to break down glucose... so really the net gain of ATP molecules is 2..