MANuALv1.0 06/ 20/ 2001 R: 06/ 20/ 2001 I fi: 00 PROJECT NO.: WIL 417001A SPONSOR: HAP TASK FORCE TABLE 16 (RESPONSE TO NOVEL STIMULUS) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS INDIVIDUAL CLINICAL OBSERVATIONS TABLE RANGE: 02 26 01 PAGE 1 ANIMAL SEX GROUP DATE TIME OBSERVATIONS ____________ .        ______________________________________ 61400 M 0 PFM 02 26 01 14: 16 SLIGHT REACTION 61403 M 0 PPM 02 26 01 14: 16 NOTABLY INCREASED REACTION 61404 M 0 PPM 02 26 01 14: 16 SLIGHT REACTION 61414 M 0 PPM 02 26 01 14: 16 NOTABLY INCREASED REACTION 61420 M 0 PPM 02 26 01 14: 16 NOT VISIBLE 61395 M 60 PPM 02 26 01 14: 21 NO REACTION TO STIMULUS 61398 M 60 PPM 02 26 01 14: 21 NOTABLY INCREASED REACTION 61425 M 60 PPM 02 26 01 14: 21 SLIGHT REACTION 61430 M 60 PPM 02 26 01 14: 21 SLIGHT REACTION 61435 M 60 PPM 02 26 01 14: 21 NOT VISIBLE 61407 M 205 PPM 02 26 01 14: 24 SLIGHT REACTION 61415 M 205 PPM 02 26 01 14: 24 NO REACTION TO STIMULUS 61422 M 205 PPM 02 26 01 14: 24 SLIGHT REACTION 61424 M 205 PPM 02 26 01 14: 24 NO REACTION TO STIMULUS 61433 M 205 PPM 02 26 01 14: 24 NO REACTION TO STIMULUS 61418 M 1474 PPM 02 26 01 * NO REACTION TO STIMULUS 61423 M 1474 PPM 02 26 01 * NO REACTION TO STIMULUS 61428 M 1474 PPM 02 26 01 l NO REACTION T0 STIMULUS 61429 M 1474 PPM 02 26 01 * NO REACTION TO STIMULUS 61436 M 2.474 PPM 02 26 01 * NO REACTION TO STIMULUS ______________________________ r_________ * = TIME INADVERTENTLY NOT RECORDED _________________ PROJECT NO.: WILr417001A SPONSOR: HAP TASK FORCE _____________ I__

ANIMAL SEX GROUP _________________

61450 F 61464 F 61469 F 61490 F 61494 F 61454 F 61457 F 61466 F 61487 F 61492 F 61448 F 61463 F 61468 F 61483 F 61469 F 61445 F 63.458 F 61460 F 61478 F 61485 F _______ 0 PPM 02 27 01 14: OS 0 PPM 02 27 01 14: OS 0 PPM 02 27 01 14: 05 0 PPM 02 27 01 14: OS 0 PPM 02 27 01 14: OS 45 PPM 02 27 01 14: 08 45 PPM 02 27 01 14: 08 45 PPM 02 27 01 14: 08 45 PPM 02 27 01 14: 08 45 PPM 02 27 01 14: 08 177 PPM 02 27 01 14: 12 177 PPM 02 27 01 14: 12 177 PPM 02 27 01 14: 12 177 PPM 02 27 01 14: 12 177 PPM 02 27 01 14: 12 527 PPM 02 27 01 14: 18 840 PPM 02 27 01 14: 18 840 PPM 02 27 01 14: 18 840 PPM 02 27 01 14: 18 840 PPM 02 27 01 14: 18 __.

. TABLE 16 (RESPONSE TO NOVEL STIMULUS) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS INDIVIDUAL CLINICAL OBSERVATIONS TABLE RANGE: 02 27 01 PAGE 2 ____________________________________ DATE TIME OBSERVATIONS _____________________________________

SLIGHT REACTION NOTABLY INCREASED REACTION NO REACTION TO STIMULUS NOTABLY INCREASED REACTION NO REACTION TO STIMULUS SLIGHT REACTION NO REACTION TO STIMULUS NO REACTION TO STIMULUS NO REACTION TO STIMULUS NO REACTION TO STIMULUS SLIGHT REACTION NO REACTION TO STIMULUS NO REACTION TO STIMULUS NO REACTION TO STIMULUS NO REACTION TO STIMULUS NO REACTION TO STIMULUS NO REACTION TO STIMULUS NO REACTION TO STIMULUS NO REACTION TO STIMULUS NO REACTION TO STIMULUS _____________________________________ MANuALv1.0 06, '20/ 2001 R: 10/ 29/ 2001 S S b'B1 E' Pt `TRK `8L1:

`66K `961 ' 16'1: '981 `LLT. `SLT `fLT `ELt . E9K 8SI _________________~"

K 0 hldd 0 :dflOW 3? tfW 1 33'fd (Sm3) sum3t i xaoa wnarfiraN1 SUi tl NI 33eL Z'I'T JO hanJS (ACXTIOH~ VdOLSIH HLIM) 'XOZ 'HNI 3. LKW L t 3Tw. u S 9'OK ' 09 t . PLT . L91: 'LSf '851 '9bK E S 9'6 . L.? x '6ET 'ZEK 'SZK 82X . EK. 'I:

.. 6 N 'a's NY3N PEP19 ZEbT9 6K619 OTPK9 60PT9 ?tlhlINV hQCl TABLE 17 PROJECT NO.: WIL 417001 ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1.1,2 TCE IN RATS PAGE 2 SPONSOR: H? iP TASK FORCE INDIVIDUAL BODY WEIGHTS (GRAMS)

MALE GROUP: 58 PPM DAY 9 3 0 1 _______________________________________ ANIMAL 61396 133. 166. 181. 180. 61405 125. 160. 174. 178. 61413 113. 143. 157. 160. 61416 137. 175. 197. 197. 61431 124. 152. 167. 169.

MEAN 126. 159. 175. 177. S. D. 9.3 12.4 15.1 13.8 N 5 5 5 5 PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE 9 ____ DAY _ ANIMAL 61399 61411 61441 61442 61443 MALE GROUP: 181 PPM 3 0 1 ______________________________ I_"

124. 157. 173. 165. 127. 161. 179. 172. 128. 162. 182. 177. 132. 169. 186. 178. 115. 148. 167. 167.

MEAN 125. 159. 177. 172. S. D. 6.4 7.7 7.5 5.8 N 5 5 5 5 TABLE 17 ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS INDIVIDUAL BODY WEIGHTS (GRAMS) PAGE 3 123 TABLE 17 PROJECT NO.: WIL 417001 ACUTE INH. TOX. (WITH HISTOPATHOL~ Y) STUDY OF ~,~, Z TCE IN RATS SPONSOR: HAP TASK FORCE INDIVIIJUAL BODY WEIGHTS (GRAMS) PAGE 5 FEMALE GROUP: 0 PPM DAY 9 3 0 1 ____________________

ANIMAL 61446 108. 119. 130. 132. 61452 105. 121. X28. 130. 61453 100. 109. 115. 114. 61456 100. 114. 121. 122. 61491 105. 124. 129. 130.

MEAN 104. 117. 125. 126. S. D. 3.5 5.9 6.4 7.5 N 5 5 5 5 PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 17 ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS INDIVIDUAL BODY WEIGHTS (GRAMS) PAGE 6 FEMALE GROUP: 58 PPM DAY 9 3 0 1 ______________________________________ L_

ANIMAL 61467 95. 111. 115. 117.

61470 100. 112. 118. 119. 61472 102. 121. 129. 131. 61475 108. 122. 132. 130. 61477 99. 116. 126. 127.

MEAN 101. 116. 12.4. 125.

S. D. 4.8 5.0 7.2 6.4 N 5 5 5 5 I. 26 I. 27 , , I I , I t , I I / 2 / I / I , I , / I , , 1 I , i , , ,

rl w H 128 129 TABLE 17 PROJECT NO.: WIL 417001A ACUTE INH. TOX (WITH HIST~ PATHOLOGY) STUDY OF 1,1,2 TCE IN RATS SPONSOR: HAP TASK FORCE INDIVIDUAL BODY WEIGHTS (GRAMS) PAGE 3   _ _    _ _ ANIMAL 61407 61415 61422 61424 61433 9 _____ 3 __"_____

124. 154. 134. 168. 115. 148. 133. 172. 122. 158.

MEAN 126. 160. S. D. 8.0 9.9 N 5 5 MALE GROUP: 205/ 177 PPM 0 1 .________________"~

171. 166. 190. 184. 171. 165. 197. 190. 186. 179.

183. 177. 11.6 11.0 5 5 TABLE 17 PROJECT NO.: WIL 417001A ACUTE INH. TOX (WITH HIST~ PATHOLOGY) STUDY OF 1,1,2 TCE IN RATS SPONSOR: HAP TASK FORCE INDIVIDUAL BODY WEIGHTS (GRAMS) PAGE 4 MALE GROUP: 14741840 PPM DAY 9 3 0 1 ____________________ L___________________

ANIMAL 61418 124. 160. 182. 159. 61423 128. 162. 185. 160. 61428 119. 152. 178. 152. 61429 141. 173. 200. 173. 61436 119. 148. 173. 149.

MEAN 126. 159. 184. 159. S. D. 9.1 9.7 10.2 9.3 N 5 5 5 5 __._  ___  __  _______ TABLE 17 PROJECT NO.: WIL 417001A ACUTE INH. TOX (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS SPONSOR: HAP TASK FORCE INDIVIDUAL BODY WEIGHTS (GRAMS) PAGE 5 FEMALE GROUP: 0 PPM DAY 9 3 0 1 ___________________

ANIMAL 61450 109. 125. 135. 136. 61464 93. 111. 124. 124. 61469 96. 112. 122. 123. 61490 102. 116. 131. 132. 61494 105. 119. 131. 133.

ME?. N 101. 117. 129. 130. S. D. 6.5 5.7 5.4 5.8 N 5 5 5 5 TABLE 17 PROJECT NO.: WIL 417001A ACUTE INH. TOX (WITH HI. STOPATHOLO(; Y) STUDY OF 1,1,2 TCE IN RATS SPONSOR: HAP TASK FORCE INDIVIDUAL BODY WEIGHTS (GRAMS)

FEMALE GROUP: 60/ 45 PPM DAY 9 3 0 1 ________________________________________~~

ANIMAL 61454 109. 127. 144. 139. 61457 98. 110. 116. 118. 61466 104. 118. 132. 127. 61487 107. 121. 134. 132. 61492 94. 112. 124. 123.

MEAN 102. 118. 130. 128. S. D. 6.3 6.9 10.6 8.1 N 5 5 5 5 PAGE 6 PAGE 7 TABLE 17 PROJECT NO.: WIL 417001A ACUTE INH. TOX (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS SPONSOR: HAP TASK FORCE INDIVIDUAL BODY WEIGHTS (GRAMS)

FEMALE GROUP: 205/ 111 PPM DAY 3 3 0 1 ________________________________________

ANIMAL 61448 110. 121. 134. 129.

61463 92. 113. 125. 115.

61468 33. 107. 119. 112.

61483 114. 127. 138. 132.

61489 104. 118. 129. 121.

MERN 103. 117. 129. 122.

S. D. 3.3 7.6 1.4 8.6 N 5 5 5 5 TABLE 17 PROJECT NO.: WIL 41700J. A ACUTE INH. TOX (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS SPONSOR: HAP TASK FORCE INDIVIDUAL BODY WEIGHTS (GRnMS) PAGE 8 FEMALE GROUP: 1474/ 840 PPM DAY 9 3 0 1 ___________ s___________________________________________________________________"________ I__._________________________~

ANIMAL 61445 105. 119. 131. 119. 61458 96. 114. 126. NA 61460 105. 119. 132. 117. 61478 108. 122. 133. 121. 61485 98. 110. 118. 108.

MEAN 102. 117. 128. 116. S. D. 5.1 4.8 6.2 5.7 N 5 S 5 4 NA = NOT APPLICABLE PBFTSv4.13 06/ 12/ 2001 136 138 139 TABLE 18 PROJECT NO.: WIL 417001 ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS SPONSOR: HAP TASK FORCE INDIVIDUAL BODY WEIGHT GAINS (GRAMS) PAGE 5 FEMALE GROUP: 0 PPM DAY 9 TO 3 3T0 0 OTO 1 _______________________________ c_______ ANIMAL 61446 11. 11. 2. 61452 16. 7. 2. 61453 9. 6. 1. 61456 14. 7. 1. 61491 19. 5. 1.

MEAN 14. 7. 1. S. D. 4.0 2.3 1.2 N 5 5 5 PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 18 ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS INDIVIDUAL BODY WEIGHT GAINS (GRAMS) PAGE 6 FEMALE GROUP: 58 PPM DAY 9 TO 3 3T0 0 OTO 1 _______________"____

ANIMAL 61467 16. 4. 2. 61470 12. 6. 1. 61472 3.9. 8. 2. 61475 14. 10. 2. 61477 17. 10. 1.

MEAN 16. 8. 1. S. D. 2.7 2.6 1.6 N 5 5 5 PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 18 ACUTE INH. TAX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS INDIVIDUAL BODY WEIGHT GAINS (GRAMS) PAGE 7 FEMALE GROUP: 181 PPM DAY 9 TO 3 3T0 0 OTO 1 __"________ s____________________________~

ANIMAL 61449 13. 11. 61474 12. 3. 61482 15. 9. 61486 17. 7. 61493 17. 7. 7. 3. 5. 7. 6.

MEAN 15. 7. 6. S. D. 2.3 3.0 1.7 N 5 5 5 PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE DAY 9 TO 3 3T0 0 ____________________

ANIMAL 61451 19. 7. 61462 12. 10. 61476 14. 8. 61481 12. 9. 61484 13. 8.

MEAN 14. 8. S. D. 2.9 1.1 N 5 5 NA = NOT APPLICABLE TABLE 18 ACUTE INH. TOX. (WITH HISTOPATHOLCGY) STUDY OF 1,1,2 TCE IN RATS INDIVIDUAL BODY WEIGHT GAINS (GRAMS) PAGE 0 FEMALE GROUP: 1527 PPM OTO 1 _______^_________"

NA 12. 12. NA 16.

13. 2.3 3 PBFTSv4.13 06/ 12/ 2001 PROJECT NO.: WIL 417001A SPONSOR: HAP TASK FORCE TABLE 18 ACUTE INH. TOX (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS INDIVIDUAL BODY WEIGHT GAINS (GRAMS) PAGE 1 MALE GROUP: 0 PPM DAY 9 TO 3 3T0 0 OTO 1 _____________________________________"~.~

ANIMAL 61400 29. 24. 1. 61403 36. 20. 1. 61404 30. 19. 2. 61414 29. 20. 0. 61420 4l.. 25. 0.

MEAN 33. 22. 0. S. D. 5.3 2.7 1.1 N 5 5 5 TABLE 18 PROJECT NO.: WIL 417001A ACUTE INH. TOX (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RnTS SPONSOR: HAP TASK FORCE INDIVIDUAL BODY WEIGHT GAINS (GRAMS) PAGE 2 MALE GROUP: 60/ 45 PPM DAY 9 TO 3 3T0 0 OTO 1 ____________________.~

ANIMAL 61395 36. 61398 32. 61425 32. 61430 31. 61435 37. 19. 1. 17. 1. 20. 1. 22. 0. 26. 6.

MEAN 34. 21. 1. S. D. 2.1 3.4 2.1 N 5 5 5 TABLE 18 PROJECT NO.: WIL 417001A ACUTE INH. TOX (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS SPONSOR: HAP TASK FORCE INDIVIDUAL BODY WEIGHT GAINS (GRAMS) PAGE 3 MALE GROUP: 205/ 177 PPM DAY 9 TO 3 3T0 0 OTO 1 ________________________________________~

ANIMAL 61407 30. 17. 5. 61415 34. 22. 6. 61422 33. 23. 6. 61424 39. 25. 7. 61433 36. 28. 7.

MEAN 34. 23. 6. S. D. 3.4 4.1 0.8 N 5 5 5 TABLE 18 PROJECT NO.: WIL 417001A ACUTE INH. TOX (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS SPONSOR: HAP TASK FORCE INDIVIDUAL BODY WEIGHT GAINS (GRAMS) PAGE 4 MALE GROUP: 1474/ 840 PPM DAY 9 TO 3 3T0 0 OTO 1 ________________________________________~

ANIMAL 61418 36. 22. 23. 61423 34. 23. 25. 61428 33. 26. 26. 61429 32. 27. 27. 61436 29. 25. 24.

MEAN 33. 25. 25.

S. D. 2.6 2.1 1.6 N 5 5 5 TABLE 18 PROJECT NO.: WIL 417001A ACUTE INH. TOX (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS SPONSOR: HAP TASK FORCE INDIVIDUAL BODY WEIGHT GAINS (GRAMS) PAGE 5 DAY 9 TO 3 ______________ ANIMAL 61450 16. 61464 18. 61469 16. 61490 14. 61494 14. FEMALE GROUP: 0 PPM 3T0 0 OTO 1 ________ L___________ _________________

10. 1. 13. 0. 10. 1. 15. 1.

12. 2.

MEAN 16. 12. 1. S. D. 1.7 2.1 0.7 N 5 5 5 TABLE 18 PROJECT NO.: WIL 417001A ACUTE INA. TOX (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS SPONSOR: HAP TASK FORCE INDIVIDUAL BODY WEIGHT GAINS (GRAMS)

FEMALE GROUP: 60/ 4S PPM DAY 9 TO 3 3T0 0 OTO 1 ____________________ ANIMAL 61454 18. 17. 5. 61457 12. 6. 2. 61466 14. 14. 5. 61487 14. 13. 2. 61492 18. 12. 1.

MEAN 15. 12. 2. S. D. 2.7 4.0 2.9 N 5 5 5 PAGE 6 ,

150 TABLE 18 PROJECT NO.: WIL 417001A ACUTE INH. TOX (WITH HISTOPATKOLOGY) STUDY OF 1,1,2 TCE IN RATS SPONSOR: HAP TASK FORCE INDIVIDUAL BODY WEIGHT GAINS (GRAMS)

FEMALE GROUP: 1474/ 840 PPM DAY 9 TO 3 3T0 0 OTO 1 ________________________________________ ___________________

ANIMAL 61445 14. 12. 12. 61458 18. 12. NA 61460 14. 13. 15. 61478 14. 11. 12. 61485 12. 8. 10.

MEAN 14. 11. 12. S. D. 2.2 1.9 2.1 N 5 5 4 NA = NOT APPLICABLE PAGE 8 PBFTSv4.13 06/ 12/ 2001 PROJECT NO.: WIL 417001A SPONSOR : :HAP TASK FORCE TABLE 19 ACUTE INH. TOX (WITH HISTOPATHOLCGY) STUDY OF l, l, z TIE IN RATS INDIVIDUAL BRONCHOALVEOLAR LAVAGE VALUES PAGE 1 DAY 1 ________________________________________________ r________________________________________, r_ l_________________

TOTAL TVOL TOTALCEL ANIMAL PROTEIN COUNT ___________________"

mg/ dL nfL x10 t/ ml, ________"_______________~~~_________________________________________________________________"_____ r_________________________ GROUP: 0 PPM MALES 61400 a. 4 4. 1.5 61403 7.9 3. 1.7 61404 9.0 4. 2.3 61414 a. 0 4. 1.7 61420 10.3 3. 1.4 MEAN a. 7 4. 1.7 S. D. 0.98 0.5 0.35 N 5 5 5 ______________________________ L______ mg/ dL = GRAMS/ DECILITER, II& = MILLILITERS PROJECT NO.: WIL 417001A SPONSOR: HAP TASK FORCE TABLE 19 ACUTE INH. TOX (WITH HISTOPATHOLCGY) STUDY OF l, l, Z TCE IN RATS INDIVIDUAL BRONCHOALVEOLAR LAVAGE VALUES PAGE 2 DAY 1 ___________________ TOTAL TVOL TOTALCEL ANIMAL PROTEIN COUNT ____________________"

w/ dL mL x10 6/ 11& ____________________" GROUP: 60/ 45 PPM MALES 61395 LA LA LA 61398 12.0 3. 1.9 61425 8.6 4. 1.4 61430 LA LA LA 61435 10.0 3. 0.9 MEAN 10.2 3. 1.4 S. D. 1.71 0.6 0.50 N 3 3 3 ________________________________________ r LA = LAB ACCIDENT _______________________________________  mg/ dL = GRAMS/ DECILITER, mL = MILLILITERS TABLE 19 PROJECT NO.: WIL 417001A ACUTE INH. TOX (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS PAGE 3 SPONSOR: HAP TASK FORCE INDIVIDUAL BRONCHOALVEOLAR LAVAGE VALUES DAY 1 ____________________ I___________________~

TOTAL TVOL TOTALCEL ANIMAL PROTEIN COUNT __________________ L_____________________

w/ dL mu %10" 6/ mL _______________"_______________________

GROUP: 205/ 177 PPM MALES 61407 13.8 2. 1.8 61415 LA LA LA 61422 8.1 4. 1.6 61424 9.5 4. 1.5 61433 9.7 4. 1.2 MEAN 10.3 4. 1.5 S. D. 2.46 1.0 0.25 N 4 4 4 _______~ LA = LAB ACCIDENT mg/ dL = GRAMS/ DECILITER, mL = MILLILITERS PROJECT NO.: WIL 417001A SPONSOR: HAP TASK FORCE TABLE 19 ACUTE INN. TOX (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS INDIVIDUAL BRONCHOALVEOLAR LAVAGE VALUES PAGE 4 DAY 1 ________________________________________"

TOTAL TVOL TOTALCEL ANIMAL PROTEIN COUNT ________________________________________

w/ a WJ x10 6/ ti ___________________~

GROUP: 1474/ 840 PPM MALES 61418 23.9 4. 1.2 61423 25.6 3. 0.7 61428 27.4 3. 1.0 61429 22.0 4. 1.7 61436 17.0 3. 1.2 MEAN 23.2 3. 1.2 S. D. 3.99 0.5 0.36 N 5 5 5 _________________

mg/ dL = GRAMS/ DECILITER, mL = MILLILITERS 156 . . . . . rnrnrnrnN 157 I. 58 159 TABLE 20 (UNSCHEDULED mxms) PROJECT NO.: WIL 417001 ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF l, l, Z TCE IN RATS PAGE 1 SPONSOR: HAP TASK FORCE GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS ANIMAL NO. 61451 GROUP 4: 1527 PPM FEMALE FOUND DEAD 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 __________________ KIDNEYS FINAL BODY WT( G1 123.

LIVER LARYNX NASAL LEVEL II NASAL LEVEL 113 NASAL LEVEL IV NASAL LEVEL V GRADE _________________" MICRO: NECROSIS, TUBULAR 1 LUMINAL DEBRIS 1 SMALL AMOUNT OF BRIGHTLY EOSINOPHILIC GRANULAR MATERIAL WITHIN CORTICAL TUBULE5 THAT WERE NOT NECROTIC. MICRO: NECROSIS, HEPATOCELLULAR, CENTRILOBULAR 4 BRIDGING CENTRILOBULAR NECROSIS AND HEMORRHAGE. MICRO: INFLAMMATION, ACUTE 1 ACUTE TO SUBACUTE INFLAMMATION NEAR THE BASE OF THE EPIGLOTTIS AND VENTRAL POUCH. VOCAL PROCESSES NOT WELL PRESENTED IN SECTION, RECUT SECTIONS WERE EXAMINED. MICRO: INFLAMMATION, SUBACUTE 1 LATERAL WALL OPPOSITE THE MAXILLOTURBINATES. NECROSIS, SINGLE CELL 1 NECROSIS ON THE TURBINATES, PRIMARILY THE NASOTURBINATES. MICRO: NECROSIS, OLFACTORY EPITHELIUM 3 NECROSIS AND LOSS OF EPITHELIUM AND UNDERLYING GLANDS. MICRO: NECROSIS, OLFACTORY EPITHELIUM 2 NECROSIS AND LOSS OF EPITHELIUM AND UNDERLYING GLANDS, DORSAL MFATUS AND ETHMOID TURBINATES. MICRO: NECROSIS, OLFACTORY EPITHELIUM WIDELY SCATTERED SINGLE CELL NECROSIS. 1 PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 20 (UNSCHEDULED DEATHS) ACUTE INH. TOX. (WITH HISTOPATHOLCGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 2 ANIMAL NO. 61451 GROUP 4: 1527 PPM FEMALE FOUND DEAD 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ________________________________________

NASAL LEVEL VI MICRO: NECROSIS, OLFACTORY EPITHELIUM 1 DIFFUSELY SCATTERED SINGLE CELL NECROSIS. CAUSE DEATH/ MORB MICRO: UNDETERMINED P SEVERE HEPATOCELLULAR NECROSIS IS A POSSIBLE CAUSE OF DEATH, BUT BECAUSE OF THE LIMITED NUMBER OF TISSUES EXAMINED A DEFINITIVE CAUSE OF DEATH WAS NOT DETERMINED. NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL C&? WDS STERNEBRAE BRAIN CECUM COLON DUODENUM AORTA ESOPHAGUS EYES/ OPTIC N. OVIDUCTS LYMPH NODE, TR/ BR HEART ILEUM JEJUNUM KIDNEYS LIVER LYMPH NODE, MED NASAL TISSUE LARYNX LUNGS THYMUSGLAND NERVB, SCIATIC OVARIES PANCREAS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA MICRO: ADRENAL CORTEX ADRENAL MEDULLA LUNGS STOMACH, GLD TRACHEA NASAL LEVEL I GROSS GRADE CODE: l SLIGHT, % MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, 2 MILD, 3 MODERATE. 4 SEVERE, P PRESENT PAGE 3 PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 20 (UNSCHEDULED DEATHS) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS ANIMAL NO. 61462 GROUP 4: 1527 PPM FEMALE FOUND DEAD 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ______ L____________*~

KIDNEYS FINAL BODY WT( G) 117.

LIVER LARYNX MICRO: LUMINAL DEBRIS 1 SKIN SKIN STOMACH, GLD NASAL LEVEL II NASAL LEVEL 111 NASAL LEVEL IV NASAL LEVEL V SMALL AMOUNT OF BRIGHTLY EOSINOPHILIC GRANULAR MATERIAL WITHIN CORTICAL TUBULES THAT WERE NOT NECROTIC. MICRO: NECROSIS, HEPATOCELLULAR, CENTRILOBULAR BRIDGING CENTRILOBULAR NECROSIS AND HEMORRHAGE. MICRO: INFLAMMATION, ACUTE ACUTE TO SUSACU! TE INFLAMMATION, VENTRAL POUCH AND DISTAL LARYNX. THE VOCAL PROCESSES WERE NOT WELL PRESENTED IN SECTION, RECUTS WERE EXAMINED. GROSS: RED MATTING OCULAR, RIGHT; NASAL GROSS: CLEAR MATTING OCUI. J+ R, BILATERRL MICRO: ULCERATION MICRO: NECROSIS, SINGLE CELL TRANSITIONAL EPITHELIUM OF TURBINATES AND LATERAL WALL. MICRO: NECROSIS, OLFACTORY EPITHELIUM NECROSIS AND LOSS OF EPITHELIUM AND UNDERLYING GLANDS. MICRO: NECROSIS, OLFACTORY EPITHELIUM NECROSIS AND LOSS OF EPITHELIUM AND UNDERLYING GLANDS, DORSAL MEATUS AND ETHMOID TURBINATES. MICRO: NECROSIS, OLFACTORY EPITHELIUM 4 1 2 PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE mm3 20 UJNSCHEDULED mmw ACUTE INH. TOX. (WITH HISTOFATHOLCGYt STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 4 ANIMAL NO. 61462 GROUP 4: 1527 PPM FEMALE FOUND DEAD 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ______________________________________~ NECROSIS AND LOSS OF THE EPITHELIUM AND UNDERLYING GLANDS, DORSAL MEATUS AND FACING TURBINATES AND DORAL SEPTUM. NASAL LEVEL VI MICRO: NECROSIS, OLFACTORY EPITHELIUM 2 CAUSE DEATH/ MORE MICRO: UNDETERMINED P SEVERE HEPATOCELLULAR NECROSIS IS A POSSIBLE CAUSE OF DEATH, BUT BECAUSE OF THE LIMITED NUMBER OF TISSUES EXAMINED A DEFINITIVE CAUSE OF DEATH WAS NOT DETERMINED. NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNEBRAE BRAIN CECUM COLON DUODENUM AORTA ESOPHAGUS EYES/ OPTIC N. OVIDUCTS LYMPH NODE, TR/ BR HEART ILEUM JEJUNUM KIDNEYS LIVER LYMPH NODE, MED NASAL TISSUE LARYNX LUNGS THYMUS GLAND NERVE, SCIATIC OVARIES PANCREAS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA MICRO: ADRENAL CORTEX ADRENALMEDULLA LUNGS TRACHEA NASAL LEVEL I GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, 2 MILD, 3 MODERATE, I SEVERE. P PRESENT TABLE 20 (UNSCHEDULED DEATHS) PROJECT NO.: WIL 417001 ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS PAGE 5 SPONSOR: HAP TASK FORCE GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS ANIMAL NO. 61481 GROUP 4: 1527 PPM FEMALE FOUNDDEAD 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE . ___________________~ LIVER FINAL BODY WT( G) 113.. LARYNX STOMACH STOMACH, GLD NASAL LEVEL II NASAL LEVEL III ___________ 1___ 11_

MICRO: NECROSIS, HEPATOCELLULAR, CENTRILOBULAR 4 MICRO: INFLAMMATION, ACUTE 2 ACUTE TO SUBACUTE INFLAMMATION, VENTRAL POUCH AND VOCAL PROCESSES. THE BASE OF THE EPIGLOTTIS WAS NOT WELL PRESENTED IN SECTION, RECUT SECTIONS WERE EXAMINED. EDEMA 1 GROSS: DARK RED AREA( S) P ONE, 3 X 1 MM, GLANDULAR PORTION NASAL LEVEL IV NASAL LEVEL V NASAL LEVEL VI CAUSE DF. ATH/ MORB MICRO: NO SIGNIFICANT CHANGES OBSERVED NO HISTOLGGIC CORRELATION FOR DARK RED AREAS. MICRO: NECROSIS, SINGLE CELL 1 TRANSITIONAL EPITHELIUM OF TURBINATES AND LATERAL WALL. MICRO: NECROSIS, OLFACTORY EPITHELIUM 3 NECROSIS AND LOSS OF EPITHELIUM AND UNDERLYING GLANDS. NECROSIS, SINGLE CELL 1 CONTINUED INVOLVEMENT OF TRANSITIONAL EPITHELIUM, TURBINATES AND LATERAL WALL. MICRO: NECROSIS, OLFACTORY EPITHELIUM 4 MICRO: NECROSIS, OLFACTORY EPITHELIUM 2 MICRO: NECROSIS, OLFACTORY LPITHELIUM 2 MICRO: UNDETERMINED P SEVERE HEPATOCELLULAR NECROSIS IS A POSSIBLE CAUSE OF DEATH, BUT BECAUSE OF THE LIMITED NUMBER OF TISSUES EXAMINED A PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE mm3 20 (UNSCHEDULED DExm. 9 ACUTE INH. TOX. (WITH HISTOPATHOLCGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 6 ANIMAL NO. 61481 GROUP 4: 1527 PPM FEMALE FOUND DEAD 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE _____________________________________~~" DEFINITIVE CAUSE OF DEATH WAS NOT DETERMINED. NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNEBRAE BRAIN CECUM COLON DUODENUM AORTA ESOPHAGUS EYES/ OPTIC N. OVIDUCTS LYMPH NODE, TR/ BR HEART ILEUM JEJUNUM KIDNEYS LIVER LYMPH NODE, MED NASAL TISSUE LARYNX LUNGS THYMUSGLAND NERVE, SCIATIC OVARIES PANCREAS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA MICRO: ADRENAL CORTEX ADRENAL MEDULLA KIDNEYS LUNGS STOMACH, GLD STOMACH, NONGLD TRACHEA NASAL LEVEL I GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, 2 MILD, 3 MODERATE, 4 SEVERE, P PRESENT PGRHv4.28 10/ 29/ 2001 PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 1 ANIMAL NO. 61409 GROUP 1: 0 PPM MALE __________ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS TESTES ADRENAL GLANDS FINAL BODY WT( G) ABS. (G) 1.67 7.56 1.36 0.90 2.02 0.0388 163. _________ REL. PHARYNX 1.025 PANCREAS 4.638 0.834 TRACHEA 0.552 NASAL LEVEL II 1.239 0.024 NO SIGNIFICANT CHANGES OBSERVED SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ___________~ MICRO: INFLAMMATION, SUBACUTE 1 MICRO: ATROPHY, ACINAR 1 TISSUE IN PLANE WITH GLANDULAR STOMACH. MICRO: INFILTRATE, LYMPHOCYTE 1 MICRO: INFLAMMATION, SUBACUTE 1 LATERAL WALL OPPOSITE THE MAXILLOTURBINATES.

GRO~ S: ADRENAL GLANDS STERNEBRAE COLON DUODENUN ESOPHAGUS EYES/ OPTIC N. ILEUM JEJUNUM LYMPH NODE, MED NASAL TISSUE LUNGS THYMUS GLAND RECTUM PITUITARY SEMINAL VESICLES SKELETAL MUSCLE SPLEEN STOMACH URINARY BLADDER MICRO: ADRENAL CORTEX ADRENAL MEDULLA LARYNX LUNGS NASAL LEVEL III NASAL LEVEL IV BRAIN CECIJM AORTA EPIDIDYNIDES LYMPH NODE, TR/ BR HEART KIDNEYS LIVER LARYNX TESTES NERVE, SCIATIC PANCREAS PROSTATE SAL. GLANDMAND SKIN SPINAL CORD THYROID GLANDS TRACHEA KIDNEYS LIVER STOMACH, GLD NASAL LEVEL I NASAL LEVEL V NASAL LEVEL VI GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, 2 MILD, 3 MODERATE, 4 SEVERE, P PRESENT PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS PAGE 2 GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS ANIMAL NO. 61410 GROUP 1:

________________  L_

ORGAN WEIGHT ABS. (G) BRAIN 1.71 LIVER 7.27 KIDNEYS 1.46 LUNGS 1.03 TESTES 1.70 ADRENAL GLANDS 0.0370 FINAL BODY WI( G) 173. REL.

0.988 4.202 0.844 0.595 0.983 0.021 0 PPM MALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE _______

KIDNEYS MICRO: NEPHROPATHY 1 LIVER MICRO: INFILTRATE, MONONUCLEAR 1 LARYNX MICRO: NO SIGNIFICANT CHANGES OBSERVED VOCAL PROCESSES WERE NOT WELL PRESENTED IN SECTION, RECUT SECTIONS WERE EXAMINED. NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNEBRAE BRAIN CECUM COLON DUODENUM AORTA EPIDIDYMIDES ESOPHAGUS EYES/ OPTIC N. LYMPH NODE. TR/ BR HEART ILEUM JEJUNUM KIDNEYS LIVER LYMPH NODE, MED NASAL TISSUE LARYNX TESTES LUNGS THYMUSGLAND NERVE, SCIATIC PANCREAS RECTUM PITUITARY PROSTATE SAL. GLAND MAND SEMINAL VESICLES SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER MICRO: ADRENAL CORTEX ADRENAJ., MEDULLA LARYNX LUNGS STOMACH, GLD TRACHEA NASAL LEVEL I NASAL LEVEL II NASAL LEVEL III NASAL LEVEL IV NASAL LEVEL V NASAL LEVEL VI GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: ~ MINIMAL, ~ MILD, ~ MODERATE, ~ SEVERE, P PRESENT PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 3 ANIMAL NO. 61419 GROUP 1: 0 PPM MALE ______________ .

ORGAN WEIGHT ABS. (G) BRAIN 1.66 LIVER a. 12 KIDNEYS 1.56 LUNGS 0.89 TESTES 1.82 ADRENAL GLANDS 0.0398 FINAL BODY NT( G) 177. ____ c_________________

REL. LARYNX 0.938 4.588 0.881 NO SIGNIFICANT 0.503 CHANGES OBSERVED 1.028 0.022 SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ______________________________________ MICRO: NO SIGNIFICANT CHANGES OBSERVED BASE OF THE EPIGLOTTIS NOT WELL PRESENTED IN SECTION, RECUT SECTIONS WERE EXAMINED.

GROSS: ADRENAL GLANDS COLON ESOPHAGUS ILEUM LYMPH NODE, MED LUNGS RECTUM SEMINAL VESICLES SPLEEN URINARY BLADDER MICRO: ADRENAL CORTEX LARYNX NASAL LEVEL I NASAL LEVEL V STERNEBRAE BRAIN CECUM DUODENUM AORTA EPIDIDYMIDES EYES/ OPTIC N. LYMPH NODE, TR/ BR HEART JEJUNM KIDNEYS LIVER NASAL TISSUE LARYNX TESTES THYMUS GLAND NERVE, SCIATIC PANCREAS PITUITARY PROSTATE SAL. GLANDMAND SKELETAL MUSCLE SKIN SPINAL CORD STOMACH THYROID GLANDS TRACHEA ADRENAL MEDULLA LUNGS NASAL LEVEL II NASAL LEVEL VI KIDNEYS STOMACH, GLD NASAL LEVEL III LIVER TRACHEA NASAL LEVEL IV GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, Z MILD, 3 MODERATE, 4 SEVERE, P PRESENT PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLCGY) STUDY OF l, l, P TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 4 ANIMAL NO. 61432 GROUP 1: 0 PPM MALE _______________ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS TESTES ADRENAL GLANDS FINAL BODY WT( G)  __ ABS. (G)

1.67 7.83 1.57 0.89 2.26 0.0318 191. ,_______ REL. KIDNEYS 0.874 LIVER 4.099 NASAL LEVEL II 0.822 0.466 1.183 0.017 NO SIGNIFICANT CHANGES OBSERVED SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE .________"~ MICRO: NEPHROPATHY 1 MICRO: INFILTRATE, MONONUCLEAR 1 MICRO: INFLAMMATION, SUBACUTE 1 TRANSITIONAL EPITHELIUM OF TURBINATES AND LATERAL WALL. NECROSIS, SINGLE CELL 1 LATERAL WALL OPPOSITE THE MAXILLOTURBINATES.

GROSS: ADRENAL GLANDS COLON ESOPHAGUS ILEUM LMulpH NODE, MED LUNGS RECTUM SEMINAL VESICLES SPLEEN URINARY BLADDER MICRO: ADRENAL CORTEX STOMACH, GLD NASAL LEVEL IV STERNEBRAE BRAIN CECUM DUODENUM AORTA EPIDIDYMIDES EYES/ OPTIC N. LYMPH NODE. TR/ BR HEART mJuNuM KIDNEYS LIVER NASAL TISSUE LARYNX TESTES THYMUSGLAND NERVE, SCIATIC PANCREAS PITUITARY PROSTATE SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD STOMACH THYROID GLANDS TRACHEA ADRENAL MEDULLA TRACHEA NASAL LEVEL V LARYNX NASAL LEVEL I NASAL LEVEL VI LUNGS NASAL LEVEL III GROSS GRADE CODE: l SLIGHT, ~ MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, Z MILD, 3 MODERATE, 4 SEVERE, P PRESENT PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 5 ANIMAL NO. 61434 GROUP 1:

____________ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS TESTES ADRENAL GLANDS FINAL BODY NT( G)        _  _

ABS. (G) 1.83 8.84 1.62 1.11 2.20 0.0405 199. .__ _ REL. 0.920 4.442 0.814 0.558 1.106 0.020 0 PPM MALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ________________________ II________

LIVER MICRO: INFILTRATE, MONONUCLEAR 1 NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNEBRAE BRAIN CECIJM COLON DUODENUM AORTA EPIDIDYMIDES ESOPHAGUS EYES/ OPTIC N. LYMPH NODE, TR/ BR HEART ILEUM JEJUNUM KIDNEYS LIVER LYMPH NODE, MED NASAL TISSUE LARYNX TESTES LUNGS THYMUSGIAND NERVE, SCIATIC PANCREAS RECTUM PITUITARY PROSTATE SAL. GIANDMAND SEMINAL VESICLES SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER MICRO: ADRENAL CORTEX ADRENAL MEDULLA KIDNEYS LARYNX LUNGS STOMACH, GLD TRACHEA NASAL LEVEL I NASAL LEVEL 11 NASAL LEVEL III NASAL LEVEL IV NASAL LEVEL V NASAL LEVEL VI GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, 2 MILD, 3 MODERATE, 4 SEVERE, P PRESENT PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS PAGE 6 GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS ANIMAL NO. 61396 GROUP 2: 58 PPM MALE ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS TESTES ADRENAL GLANDS FINAL BODY WT( G) ________ _ ABS. (G) REL. LARYNX 1.69 0.939 7.12 3.956 1.38 0.767 0.89 0.494 NASAL LEVEL II 2.09 1.161 0.0356 0.020 NO SIGNIFICANT 180. CHANGES OBSERVED SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE MICRO: INFLAMMATION, ACUTE 1 ACUTE TO SUBACUTE INFLAMMATION, DISTAL LARYNX. THE BASE OF THE EPIGLOTTIS WAS NOT WELL FRESENTED IN SECTION, RECUT SECTIONS WERE EXAMINED. MICRO: INFLAMMATION, SUBACUTE 1 LATERAL WALL OPPOSITE THE MAXILLGTURBINATES.

GROSS: ADREHAL GLANDS STERNEBRAE COLON DUODENUM ESOPHAGUS EYES/ OPTIC N. ILEUM JEJUNUM LYMPH NODE, MED NASAL TISSUE LUNGS THYMUS GLAND RECTUM PITUITARY SEMINAL VESICLES SKELETAL MUSCLE SPLEEN STOMACH URINARY BLADDER MICRO: KIDNEYS LIVER TRACHEA NASAL LEVEL III NASAL LEVEL VI BRAIN CECUM AORTA EPIDIDYMIDES LYMPH NODE, TR/ BR HEART KIDNEYS LIVER LARYNX TESTES NERVE, SCIATIC PANCRSAS PROSTATE SAL. GLANDMAND SKIN SPINAL CORD THYROID GLANDS TRACHEA STOMACH, GLD STOMACH, NONGLD NASAL LEVEL IV NASAL LEVEL V GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, 2 MILD, 3 MODERATE, I SEVERE, P PRESENT TABLE 21 (SCHEDULED NECROPSY) PROJECT NO.: WIL 417001 ACUTE INH. TOX. (WITH HIST~ PATHOL~ GY) sTuDY 0~ 1,1,2 TCE IN RATS PAGE 7 SPONSOR: HAP TASK FORCE GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS ANIMAL NO. 61405 GROUP 2: 58 PPM MALE __ ____ ___ ORGAN WEIGHT ABS. (G) BRAIN 1.72 LIVER 8.01 KIDNEYS 1.47 LUNGS 0.96 TESTES 1.95 ADRENAL GLANDS 0.0370 FINAL BODY WT( G) 178. .____ REL. LIVER 0.966 LARYNX 4.500 0.826 0.539 NASAL LEVEL IV 1.096 0.021 NASAL LEVEL VI NO SIGNIFICANT CHANGES OBSERVED SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE _____________ L__

MICRO: INFILTRATE, MONONUCLEAR 1 MICRO: NO SIGNIFICANT CHANGES OBSERVED VOCAL PROCESSES WERE NOT WELL PRESENTED IN SECTION, RECUT SECTIONS WERE EXAMINED. MICRO: INFLAMMATION, SUBACUTE 1 RESPIRATORY EPITHELIUM. MICRO: INFLAMMATION, SUBACUTE 1 INFLAMMATION, RESPIRATORY EPITHELIUM.

GROSS: ADRENAL GLANDS STERNEBRAE COLON DUODENUM ESOPHAGUS EYES/ OPTIC N. ILEUM JEJUNUM LYMPH NODE, MED NASAL TISSUE LUNGS THYMUS GLAND RECTUM PITUITARY SEMINAL VESICLES SKELETAt MUSCLE SPLEEN STOMACH URINARY BLADDER MICRO: KIDNEYS LARYNX NASAL LEVEL II NASAL LEVEL III BRAIN CECUM AORTA EPIDIDYMIDES LYMPH NODE, TR/ BR HEART KIDNEYS LIVER LARYNX TESTES NERVE, SCIATIC PANCREAS PROSTATE SAL. GLAND MAND SKIN SPINAL CORD THYROID GLANDS TRACHEA STOMACH, GLD STOMACH, NONGLD NASAL LEVEL V GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, 2 MILD, 3 MODERATE, I SEVERE, P PRESENT PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 8 ANIMAL NO. 61413 GROUP 2: 58 PPM MALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ________________________________ I_______" ORGAN WEIGHT ABS. (G) REL. KIDNEYS MICRO: NEPHROPATHY 1 BRAIN 1.67 1.044 LIVER MICRO: INFILTRATE, MONONUCLEAR 1 LIVER 6.62 4.137 LARYNX MICRO: INFLAMMATION, ACUTE 1 KIDNEYS 1.27 0.794 ACUTE TO SUBACUTE INFLAMMATION AT OR NEAR THE BASE OF THE LUNGS 0.84 0.525 EPIGLOTTIS AND VENTRAL POUCH. TESTES 1.79 1.119 ADRENAL GLANDS 0.0268 0.017 NASAL LEVEL IV MICRO: NECROSIS, OLFACTORY EPITHELIUM 1 FINAL BODY WT( G) 160. DORSAL MEATUS. NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNEBRAE BRAIN CECUM COLON DUODENUM AORTA EPIDIDYMIDES ESOPHAGUS EYES/ OPTIC N. LYMPH NODE, TR/ BR HEART ILEUM JEJUNUM KIDNEYS LIVER LYMPH NODE, MED NASAL TISSUE LARYNX TESTES LUNGS THYMUSGLAND NERVE. SCIATIC PANCREAS RECTUM PITUITARY PROSTATE SAL. GLANDMAND SEMINAL VESICLES SKELETAL MUSCLE SKIN SPINAL CDRD SPLEEN STOMACH THYROID GLANDS TRACHSA URINARY BLADDER MICRO: STOMACH, GLD STOMACH, NONGLD NASAL LEVEL II NASAL LEVEL III NASAL LEVEL V NASAL LEVEL VI GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, 2 MILD, ) MODERATE, 4 SEVERE, P PRESENT PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 9 ANIMAL NO. 61416 GROUP 2: 58 PPM MALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE _______________________________________~~ ORGAN WEIGHT ABS. (G) BRAIN 1.77 LIVER 8.89 KIDNEYS 1.75 LUNGS 1.00 TESTES 2.32 ADRENAL GLANDS 0.0435 FINAL BODY WT.( G) 197. REL. LIVER 0.898 LARYNX 4.513 0.888 0.508 NASAL LEVEL II 1.178 0.022 NASAL LEVEL IV NASAL LEVEL V NASAL LEVEL VI NO SIGNIFICANT CHANGES OBSERVED MICRO: INFILTRATE, MONONUCLEAR MICRO: INFLAMMATION, ACUTE ACUTE TO SUBACUTE INFLAMMATION, DISTAL LARYNX. EXUDATE, SUPPURATIVE MICRO: INFLAMMATION, SUBACUTE LATERAL WALL OPPOSITE THE MAXILLOTURBINATES. MICRO: NECROSIS, OLFACTORY EPITHELIUM DORSAL MEATUS. MICRO: NECROSIS, OLFACTORY EPITHELIUM SINGLE CELL NECROSIS, SEPTUM AND ADJACENT TURBINATE, UNILATERAL. MICRO: INFLAMMATION, SUBACUTE INFLAMMATION, DORSAL MEATUS.

GROSS: ADRENAL GLANDS STERNEBRAE BRAIN CECUM COLON DUODENUM AORTA EPIDIDYMIDES ESOPHAGUS EYES/ OPTIC N. LYMPH NODE, TR/ BR HEART ILEUM JEJUNUM KIDNEYS LIVER LYMPH NODE, MED NASAL TISSUE LARYNX TESTES LUNGS THYMUSGLAND NERVE, SCIATIC PANCREAS RECTUM PITUITARY PROSTATE SAL. GLANDMAND SEMINAL VESICLES SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER MICRO: KIDNEYS STOMACH, GLD STOMACH, NONGLD TRACHEA NASAL LEVEL III 175 TABLE 21 (SCHEDULED NECROPSY) PROJECT NO.: WIL 417001 ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS SPONSOR: HAP TASK FORCE GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS ANIMAL NO. 61431 GROUP 2: 58 PPM  _________ ,________"_________^_

ORGAN WEIGHT ABS. (0) REL. LARYNX BRAIN 1.74 1.030 LIVER 7.62 4.509 NASAL LEVEL II KIDNEYS 1.50 0.888 LUNGS 0.95 0.562 TESTES 1.48 0.876 ADRENAL GLANDS 0.0427 0.025 NO SIGNIFICANT FINAL BODY WT( G) 169. CHANGES OBSERVED MALE PAGE 11 SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE _"___""""____""""""""" MICRO: INFLAMMATION, ACUTE 1 ACUTE TO SUBACUTE INFLAMMATION, VENTRAL POUCH. MICRO: METAPLASIA, SQUAMOUS 1 LATERAL WALL OPPOSITE THE MAXILLOTURBINATES. INFLAMMhTION, SUBACUTE 1 LATERAL WALL OPPOSITE THE MAXILLOTURBINATES.

GROSS: ADRENAL GLANDS STERNEBRAE COLON DUODENUM ESOPHAGUS EYES/ OPTIC N. ILEUM JEJUNUM LYMPH NODE, MED NASAL TISSUE LUNGS THYMUSGLAND RECTUM PITUITARY SEMINAL VESICLES SKELETAL MUSCLE SPLEEN STOMACH URINARY BLADDER MICRO: KIDNEYS LIVER NASAL LEVEL III NASAL LEVEL IV BRAIN CECUM AORTA EPIDIDYMIDES LYMPH NODE, TR/ BR HEART KIDNEYS LIVER LARYNX TESTES NERVE, SCIATIC PANCREAS PROSTATE SAL. GLANDMAND SKIN SPINAL CORD THYROID GLANDS TRACHEA STOMACH, GLD STOMACH, NONGLD NASAL LEVEL V NASAL LEVEL VI GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, 2" MILD, 3 MODERATE, I SEVERE, P PRESENT PROJECT NO.: WIL 43.7001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INK. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 12 ANIMAL NO. 61399 GROUP 3: 181 PPM MALE _____ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS TESTES ADRENAL GLANDS FINAL BODY WT( G) L _

ABS. (G) 1.74 6.29 1.38 0.64 2.11 0.0399 165. ,_________________ REL. KIDNEYS 1.055 LIVER 3.812 LARYNX 0.836 0.509 1.279 NASAL LEVEL III 0.024 NASAL LEVEL IV NASAL LEVEL V NASAL LEVEL VI NO SIGNIFICANT CHANGES OBSERVED SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY _________________ MICRO: INFLAMMATION, CHRONIC MICRO: INFILTRATE, MONONUCLEAR MICRO: INFLAMMATION, ACUTE ACUTE TO SUBACUTE INFLAMMATION, VENTRAL POUCH. EXUDATE, SUPPURATIVE MICRO: NECROSIS, OLFACTORY EPITHELIUM MICRO: NECROSIS, OLFACTORY EPITHELIUM DORSAL MEATUS, SEPTUM, AND ETHMOID TURBINATES. MICRO: NECROSIS, OLFACTORY EPITHELIUM WIDELY SCATTERED SINGLE CELL NECROSIS. MICRO: NECROSIS, OLFACTORY EPITHELIUM SINGLE CELL NECROSIS, PRIMARILY DORSAL MEATUS AND SEPTUM.

GROSS: ADRENAL GLANDS COLON ESOPHAGUS ILEUM LYMPH NODE, MED LUNGS RECTUM SEMINAL VESICLES SPLEEN URINARY BLADDER MICRO: STOMACH, GLD STERNEBRAE BRAIN CECUM DUODENUM AORTA EPIDIDYMIDES EYES/ OPTIC N. LYMPH NODE, TR/ BR HEART JEJUNUN KIDNEYS LIVER NASAL TISSUE LARYNX TESTES THYMUS GLAND NERVE, SCIATIC PANCREAS PITUITARY PROSTATE SAL. GLANDMAND SKELETAL MUSCLE SKIN SPINAL CORD STOMACH THYROID GLANDS TRACHEA STOMACH, NONGLD DAY: 1 GRADE   _ _ _ _ _ _

1 1 1 NASALLEVEL II GROSS GRADE CODE: l SLIGHT, Z MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, 2 MILD, 3 MODERATE, 4 SEVERE, P PRESENT PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLCGY) STUDY OF 1,1,2 TCE IN FATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 13 ANIMAL NO. 61411 GROUP 3: 181 PPM MALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: L GRADE __________________~ ORGAN WEIGHT ABS. (G) BRAIN 1.68 LIVER 6.78 KIDNEYS 1.43 LUNGS 0.88 TESTES 2.02 ADRENAL GLANDS 0.04rls FINAL BODY WT( G) 172. REL. LIVER 0.977 3.942 0.831 0.512 1.174 LARYNX 0.024 NASAL LEVEL III NASAL LEVEL IV NASAL LEVEL V NASAL LEVEL VI NO SIGNIFICANT CHANGES OBSERVED MICRO: NECROSIS INFILTRATE, MONONUCLEAR INFLAMMATION, SUBACUTE MONONUCLEAR CELL INFILTRATES TO SUBACUTE INFLAMMATION AROUND CENTRAL VEINS. MICRO: NO SIGNIFICANT CHANGES OBSERVED BASE OF THE EPIGLOTTIS NOT WELL PRESENTED IN SBCTION, RECUT SECTIONS WERE EXAMINED. MICRO: NECROSIS, OLFACTORY EPITHELIUM NECROSIS AND LOSS OF EPITHELIUM AND UNDERLYING GLANDS. MICRO: NECROSIS, OLFACTORY EPITHELIUM MICRO: NECROSIS, OLFACTORY EPITHELIUM WIDELY SCATTERED SINGLE CELL NECROSIS. MICRO: NECROSIS, OLFACTORY EPITHELIUM DORSAL MEATIJS, DORSAL SEPTUM, AND TURBINATES. GLANDS SEEMED TG BE SPARED.

GROSS: ADRENAL GLANDS STERNEBFAE BRAIN CECUM COLON DUODENUM AORTA EPIDLDYMIDES ESOPHAGUS EYES/ OPTIC N. LYMPH NODE. TR/ BR HEART ILEUM JEJUNUM KIDNEYS LIVER LYMPH NODE, MED NASAL TISSUE LARYNX TESTES LUNGS THYMUS GLAND NERVE, SCIATIC PANCREAS RECTUM PITUITARY PROSTATE SAL. GLANDMAND 179 PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLGGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 15 ANIMAL NO. 61441 GROUP 3: 181 PPM MALE _ ._

ORGAN WEIGHT ABS. (G) BRAIN 1.71 LIVER 8.01 KIDNEYS 1.51 LUNGS 0.96 TESTES 2.13 ADRENAL GLANDS 0.0436 FINAL BODY WT( G) 177. ""

REL. NASAL LEVEL III 0.966 4.525 NASAL LEVEL IV 0.853 0.542 1.203 NASAL LEVEL V 0.025 NASAL LEVEL VI NO SIGNIFICANT CHANGES OBSERVED SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE _____________

MICRO: NECROSIS, OLFACTORY EPITHELIUM 2 NECROSIS AND LOSS OF EPITHELIUM AND UNDERLYING GLANDS. MICRO: NECROSIS, OLFACTORY EPITHELIUM 2 NECROSIS AND LOSS OF EPITHELIUM AND UNDERLYING GLANDS, DORSAL MEATUS AND ETHMOID TURBINATES. MICRO: NECROSIS, OLFACTORY EPITHELIUM 1 MICRO: NECROSIS, OLFACTORY EPITHELIUM 1 DIFFUSELY SCATTERED SINGLE CELL NECROSIS.

GROSS: ADRENAL GLANDS COLON ESOPHAGUS ILEUM LYMPH NODE, MED LUNGS RECTUM SEMINAL VESICLES SPLEEN URINARY BLADDER MICRO: KIDNEYS STOMACH, NONGLD STERNEBRAE BRAIN CECUM DUODENUM AORTA EPIDIDYMIDES EYES/ OPTIC N. LYMPH NODE. TR/ ER HEART JEJIJNUM KIDNEYS LIVER NASAL TISSUE LARYNX TESTES THYMUS GLAND NERVE, SCIATIC PANCREAS PITUITARY PROSTATE SAL. GLANDMAND SKELETAL MUSCLE SKIN SPINAL CORD STOMACH THYROID GLANDS TRACHEA LIVER NASAL LEVEL 11 LARYNX STOMACH, GLD GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, 2 MILD, 3 MODERATE, 4 SEVERE, P PRESENT PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 16 ANIMAL NO. 61442 GROUP 3: 181 PPM MALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ______^_____________~ ORGAN WEIGHT ABS. (G) BRAIN 1.79 LIVER 7.59 KIDNEYS 1.54 LUNGS 0.98 TESTES 2.36 ADRENAL GLANDS 0.0474 FINAL BODY WT( G) 178. REL. LIVER 1.006 4.264 0.865 0.551 IARYNX 1.326 0.027 NASAL LEVEL III NASAL LEVEL IV NASAL LEVEL V NASAL LEVEL VI NO SIGNIFICANT CHANGES OBSERVED MICRO: INFLAMMATION, SUBACUTE MONONUCLEAR CELL INFILTRATES TO SUBACUTE INFLAMMATION AROUND CENTRAL VEINS. INFILTRATE, MONONUCLEAR MICRO: INFLAMMA TION, ACUTE INFLAMMATION, FOCAL FOLDS. VOCAL PROCESSES AND BASE OF THE EPIGLOTTIS WERE NOT WELL PRESENTED IN SECTION, RECUTS WERE EXAMINED. MICRO: NECROSIS, OLFACTORY EPITHELIUM MICRO: NECROSIS, OLFACTORY EPITHELIUM MICRO: NECROSIS, OLFACTORY EPITHELIUM WIDELY SCATTERED SINGLE CELL NECROSIS. MICRO: NECROSIS, OLFACTORY EPITHELIUM DIFFUSELY SCATTERED SINGLE CELL NECROSIS.

GROSS: ADRENAL GLANDS STERNEBRAE BRAIN CECUM COLON DUODENUM AORTA EPIDIDYMIDES ESOPHAGUS EYES/ OPTIC N. LYMPH NODE. TR/ BR HEART ILEUM JEJUNUM KIDNEYS LIVER LYMPH NODE, MED NASAL TISSUE LARYNX TESTES LUNGS THYMUS GLAND NERVE, SCIATIC PANCREAS RECTUM PITUITARY PROSTATE SAL. GLAND MAND SEMINAL VESICLES SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER 182 PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOFATHOLOGY~ STUDY OF l, l, Z TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 18 ANIMAL NO. 61443 GROUP 3: 181 PPM MALE ___ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS TESTES ADRENAL GLANDS FINAL BODY WT( G)  _

ABS. (G) 1.69 7.28 1.42 0.85 1.96 0.0442 167. .C

REL. KIDNEYS 1.012 LIVER 4.359 0.850 0.509 1.174 LARYNX 0.026 NASAL LEVEL IV NASAL LEVEL V NASAL LEVELVI NO SIGNIFICANT CHANGES OBSERVED SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ________________ MICRO: INFLAMMATION, CHRONIC 1 MICRO: INFILTRATE, MONONUCLEAR 1 INFLAMMATION, SUBACUTE 1 MOST OF THE INFLAMMATION IS SUBCAPSULAR BUT SIMILAR TO THAT IN CENTRILOBULAR AREAS. MICRO: NO SIGNIFICANT CHANGES OBSERVED VOCAL PROCESSES WERE NOT WELL PRESENTED IN SECTION, RECUT SECTIONS WERE EXAMINED. MICRO: NECROSIS, OLFACTORY EPITHELIUM 2 MICRO: NECROSIS, OLFACTORY EPITHELIUM 2 MICRO: NECROSIS, OLFACTORY EPITHELXUM 1 GROSS: ADRENAL GLANDS STERNEBRAE COLON DUODENUM ESOPHAGUS EYES/ OPTIC N. ILEUM JEJUNUM LYMPH NODE, MED NASAL TISSUE LUNGS_ THYMUS GLAND RECTUM PITUITARY SEMINAL VESICLES SKELETAL MUSCLE SPLEEN STOMACH URINARY BLADDER MICRO: LARYNX STOMACH, GLD NASAL LEVEL III BRAIN CECUM AORTA EPIDIDYMIDES LYMPH NODE. TR/ BR HEART KIDNEYS LIVER LARYNX TESTES NERVE, SCIATIC PANCREAS PROSTATE SAL. GLANDMAND SKIN SPINAL CORD THYROID GLANDS TRACHEA STOMACH, NONGLD NASAL LEVEL II GROSS GRADE CODE: l SLIGHT, I MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: I MINIMAL. 2 MILD, 3 MODERATE, 4 SEVERE, P PRESENT PAGE 19 PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. [WITH HISTOPATHOLOGY) STUDY OF l, l, P TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS ANIMAL NO. 61397 GROUP 4: 1527 PPM MALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE _____________ I_____~

ORGAN WEIGHT ABS. (G) BRAIN 1.72 LIVER 7.37 KIDNEYS 1.56 LUNGS 0.90 TESTES 1.54 ADRENAL GLANDS 0.0619 FINAL BODY NT( G) 158. REL. DUODENUM 1.089 4.665 JEJUNUM 0.987 JEJUNUM 0.570 0.975 0.039 LIVER STOMACH STOMACH, GLD NASAL LEVEL II NASAL LEVEL III NASAL LEVEL IV MICRO: EROSION PROXIMAL DUODENUM, IN SECTION WITH STOMACH. GROSS: DARK RED CONTENTS MICRO: NO SIGNIFICANT CHANGES OBSERVED DARK RED CONTENTS MAY HAVE RESULTED FROM THE GASTRIC OR DUODENAL EROSIONS. MICRO: NECROSIS, HEPATOCELLULAR, CENTRILOBULAR BRIDGING CENTRILOBULAR NECROSIS, INFLAMMATION, AND HEMORRHAGE. GROSS: DARK RED CONTENTS MICRO: EROSION EROSIONS AT THE JUNCTION OF THE PYLORIS AND DUODENUM AND IS THE LIKELY CAUSE OF DARK RED CONTENTS IN THE GASTROINTESTINAL TRACT. MICRO: NECROSIS, SINGLE CELL TRANSITIONAL EPITHELIUM OF TURBINATES AND LATERAL WALL. MICRO: NECROSIS, SINGLE CELL CONTINUED INVOLVEMENT OF TRANSITIONAL EPITHELIUM, TURBINATES AND LATERAL WALL. NECROSIS, OLFACTORY EPITHELIUM NECROSIS AND LOSS OF EPITHELIUM AND UNDERLYING GLANDS. MICRO: NECROSIS, OLFACTORY EPITHELIUM PROJECT NO.: WIL 417001 SPONSOR: H? iP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 20 ANIMAL NO. 61397 GROUP 4: 1527 PPM MALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ________________________________________~~~ NECROSIS AND LOSS OF EPITHELIUM AND UNDERLYING GLANDS, DORSAL MEATUS AND ETHMOID TURBINATES. NASAL LEVEL V MICRO: NECROSIS, OLFACTORY EPITHELIUM 1 WIDELY SCATTERED SINGLE CELL NECROSIS. NASAL LEVEL VI MICRO: NECROSIS, OLFACTORY EPITHELIUM 1 DIFFUSELY SCATTERED SINGLE CELL NECROSIS. NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNEBRAE BRAIN CECUM COLON DUODENUM AORTA EPIDIDYMIDES ESOPHAGUS EYES/ OPTIC N. LYMPH NODE. TR/ BR HEART ILEUM KIDNEYS LIVER LYMPH NODE. MED NASAL TISSUE LARYNX TESTES LUNGS THYMUS GLAND NERVE, SCIATIC PANCREAS RECTUM PITUITARY PROSTATE SAL. GLAND MAND SEMINAL VESICLES SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN THYROID GLANDS TF'ACHEA URINARY BLADDER MICRO: ADRENAL CORTEX ADRENAL MEDULLA JEJUNUM KIDNEYS LARYNX LUNGS TRACHEA NASALLEVELI GROSS GRADE CODE: 1 SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, 2 MILD, 3 MODERATE, 4 SEVERE, P PRESENT PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLCGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 21 ANIMAL NO. 61406 GROUP 4: 1527 PPM MALE _. ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS TESTES ADRENAL GLANDS FINAL BODY WT.( G)  ABS. (G) REL. DUODENUM 1.59 1.046 7.11 4.678 LIVER 1.37 0.901 0.78 0.513 LARYNX 1.86 1.224 0.0469 0.031 152. STOMACH, GLD NASAL LEVEL II ..

NASAL LEVEL III NASAL LEVEL IV NASAL LEVEL V SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE __________________ MICRO: EROSION 1 PROXIMAL DUODENUM, IN SECTION WITH STOMACH. MICRO: NECROSIS, HEPATOCELLULAR, CENTRILOEULAR 3 BRIDGING CENTRILOBULAR NECROSIS, INFLAMMATION, AND HEMORRHAGE. MICRO: INFLFMMATION, ACUTE 1 ACUTE TO SUBACUTE INFLAMMATION, VENTRAL POUCH AND VOCAL PROCESSES. THE BASE OF THE EPIGLOTTIS WAS NOT NELL PRESENTED IN SECTION, RECUT SECTIONS WERE EXAMINED. MICRO: EROSION 1 MICRO: NECROSIS, SINGLE CELL 2 NECROSIS ON THE TURBINATES, PRIMARILY THE NASOTURBINATES. MICRO: NECROSIS, SINGLE CELL 2 CONTINUED INVOLVEMENT OF THE TRANSITIONAL EPITHELIUM OF BOTH TURBINATES. NECROSIS, OLFACTORY EPITHELIUM 4 NECROSIS AND LOSS OF EPITHELIUM AND UNDERLYING GLANDS. MICRO: NECROSIS, OLFACTORY EPITHELIUM 3 NECROSIS AND LOSS OF EPITHELIUM AND UNDERLYING GLANDS, DORSAL MEATUS AND ETHMOID TURBINATES. MICRO: NECROSIS, OLFACTORY EPITHELIUM 2 NECROSIS AND LOSS OF THE EPITHELIUM AND UNDERLYING GLANDS, TABLE 21 (SCHEDULED NECROPSY) PROJECT No.: WIL 417001 ACUTE INH. TOX. (WITH HISTOPATHOL~ Y) STUDY 0~ 1,1,2 TCB IN RATS PAGE 22 SPONSOR: HAP TASK FORCE GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS ANIMAL NO. 61406 GROUP 4: 1527 PPM MALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ____________________*~

DORSAL MEATUS AND FACING TURBINATES AND DORbL SEPTUM. NASAL LEVEL VI MICRO: NECROSIS, OLFACTORY EPITHELIUM 1 DORSAL MEATUS, DORSAL SEPTUM, AND TURBINATES. GLANDS SEEMED TO BE SPARED. NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL 'GLANDS STERNE5RAE COLON DUODENUM ESOPHAGUS EYES/ OPTIC N. ILEUM JEJUNUM LYMPH NODE, MED NASAL TISSUE LUNGS THYMUSGLAND RECTUM PITUITARY SEMINAL VESICLES SKELETAL MUSCLE SPLEEN STOMACH URINARY BLADDER MICRO: ADRENAL CORTEX ADRENAL MEDULLA TRACHEA NASAL LEVEL I BRAIN CECUM AORTA EPIDIDYMIDES LYMPH NODE. TR/ BR HEART KIDNEYS LIVER LARYNX TESTES NERVE, SCIATIC PANCREAS PROSTATE SAL. GLRNDMAND SKIN SPINAL CORD THYROID GLANDS TRACHEA KIDNEYS LUNGS GROSS GRADE CODE: l SLIGHT, Z MODERATE, 3 MARKED, P PRESENT MICRO GRAnE CODE: l MINIMAL, 2 MILD, 3 MODERATE, 4 SEVERE, P PRESENT TABLE 21 (SCHEDULED NECROPSY) PROJECT NO.: WIL 417001 ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS PAGE 23 SPONSOR: HAP TASK FORCE GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS ANIMAL NO. 61408 GROUP 4: 1527 PPM _"_________ c_

ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS TESTES ADRENAL GLANDS FINAL BODY WT( G) .__  _

ABS. (0) 1.74 8.37 1.77 1.07 2.13 0.0641 166. ,_ REL. ILEUM 1.048 ILEUM 5.042 1.066 JEJUNUM 0.645 JEJUNUM 1.283 0.039 LIVER LIVER LARYNX SKIN STOMACH STOMACH,

STOMACH, MALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ___________________" GROSS: DARK RED CONTENTS P MICRO: NO SIGNIFICANT CHANGES OBSERVED NO HISTOLOGIC CORRELATION TO EXPLAIN DARK RED CONTENTS. GROSS: DARK RED CONTENTS P MICRO: NO SIGNIFICANT CHANGES OBSERVED NO HISTOLOGIC CORRELATION TO EkPLAIN DARK RED CONTENTS. GROSS: DARK RED AREA( S) P MULTIPLE, IRREGULARLY SHAPED, ALL LOBES MICRO: NECROSIS, HEPATOCELLULAR, CBNTRILOBULAR 4 BRIDGING CENTRILOBULAR NECROSIS, INFLAMMATION, AND HEMORRHAGE THAT CORRESPONDS TO THE GROSS FINDING OF DARK RED AREAS. MICRO: INFLAMMATION, ACUTE 1 ACUTE TO SUBACUTE INFLAMMATION OF THE VOCAL PROCESSES, BASE OF THE EPIGLOTTIS, AND VENTRAL POUCH. GROSS: RED MATTING P BUCCAL GROSS: DARK RED AREA( S) P ONE, 1 MM IN DIAMETER, GLANDULAR; ONE, 2 MM IN DIAMETER, NONGLANDULAR GLD MICRO: NO SIGNIFICANT CHANGES OBSERVED NO HISTOLOGIC CORRELATION FOR DARK RED AREA, RECUT SECTION WAS EXAMINED. NONGLD MICRO: HEMORRHAGE 3 CORRESPONDS TO THE GROSS OBSERVATION OF DARK RED AREAS. PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLOZY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 24 ANIMAL NO. 61408 GROUP 4: 152 l PPM MALE SCHEDULED EUTH 02/ 26, '01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ___________________________________

NASAL LEVEL II MICRO: NECROSIS, SINGLE CELL TRANSITIONAL EPITHELIUM OF TURBINATES AND LATERAL WALL. NASAL LEVEL III MICRO: NECROSIS, OLFACTORY EPITHELIUM NECROSIS AND LOSS OF EPITHELIUM AND UNDERLYING GLANDS. NASAL LEVEL IV MICRO: NECROSIS, OLFACTORY EPITHELIUM NECROSIS AND LOSS OF EPITHELIUM AND UNDERLYING GLANDS, DORSAL MSATUS AND ETHMOID TURBINATES. NASAL LEVEL V MICRO: NECROSIS, OLFACTORY EPITHELIUM WIDELY SCATTERED SINGLE CELL NECROSIS. NASAL LEVEL VI MICRO: NECROSIS, OLFACTORY EPITHELIUM DIFFUSELY SCATTERED SINGLE CELL NECROSIS. NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNEBRAE BRAIN CECUM COLON DUODENUM AORTA EPIDIDYMIDES ESOPHAGUS EYES/ OPTIC N. LYMPH NODE. TR/ BR HEART KIDNEYS LYMPH NODE, MED NASAL TISSUE LARYNX TESTES LUNGS THYMUS GLAND NERVE, SCIATIC PANCREAS RECTUM PITUITARY PROSTATE SAL. GLAND MAND SEMINAL VESICLES SKELETAL MUSCLE SPINAL CORD SPLEEN THYROID GLANDS TRACHEA URINARY BLADDER MICRO: ADRENAL CORTEX ADRENAL MEDULLA ILEUM JEJUNUM KIDNEYS LUNGS STOMACH, GLD TRACHEA NASAL LEVEL I GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: 1 MINIMAL, I MILD, 3 MODERATE, 4 SEVERE, P PRESENT PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 25 ANIMAL NO. 61421 GROUP 4: 1527 PPM MALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ________________~

ORGAN WEIGHT ABS. (G) BRAIN 1.67 LIVER 6.30 KIDNEYS 1.44 LUNGS 0.83 TESTES 1.75 ADRENAL GLANDS 0.0475 FINAL BODY WT( G) 144. REL. KIDNEYS 1.160 4.375 LIVER 1.000 0.576 LARYNX 1.215 0.033 SKIN STOMACH GROSS: STOMACH, OLD MICRO:

NASAL LEVEL II MICRO:

NASAL LEVEL III MICRO:

NASAL LEVEL IV NASAL LEVEL V MICRO:

MICRO:

MICRO:

MICRO:

MICRO: LUMINAL DEBRIS BASOPHILIC GRANULAR MATERIAL WITHIN CORTICAL TuBuLAR LuMENS. NECROSIS, HEPATOCELLULAR, CENTRILOBIJLAR BRIDGING CENTRILOBULAR NECROSIS, INFLAMMATION, AND HEMORRHAGE NO SIGNIFICANT CHANGES OBSERVED BASE OF THE EPIGLOTTIS NOT WELL PRESENTED IN SECTION, REClJT SECTIONS WERE EXAMINED. RED MATTING NASAL: BUCCAL DARK RED CONTENTS NO SIGNIFICANT CHANGES OBSERVED NO HISTOLOGIC CORRELATION TO EXPLAIN DARK RED CONTENTS. NECROSIS, SINGLE CELL TRANSITIONAL EPITHELIUM OF TURBINATES AND LATERRL WALL. NECROSIS, OLFACTORY EPITHELIUM NECROSIS, SINGLE CELL CONTINUED INVOLVEMENT OF TRANSITIONAL EPITHELIUM, TURBINATES AND LATERAL WALL. NECROSIS, OLFACTORY EPITHELfUM NECROSIS AND LOSS OF EPITHELIUM AND UNDERLYING GLANDS, DORSAL MEATUS AND ETHMOID TURBINATES. NECROSIS, OLFACTORY EPITHELIUM PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLCGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 26 ANIMAL NO. 61421 GROUP 4: 1527 PFM MALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE _______________________________________"~ WIDELY SCATTERED SINGLE CELL NECROSIS. NASAL LEVEL VI MICRO: NECROSIS, OLFACTORY EPITHELIUM 1 DIFFUSELY SCATTERED SINGLE CELL NECROSIS. NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNEBRAE BRAIN CECUM COLON DUODENUM AORTA EPIDIDYMIDES ESOPHAGUS EYES/ OPTIC N. LYMPH NODE, TR/ BR HEART 'ILEUM JEJUNUM KIDNEYS LIVER LYMPH NODE, MED NASAL TISSUE LARYNX TESTES LUNGS THYMUSGLAND NERVE, SCIATIC PANCREAS RECTUM PITUITARY PROSTATE SAL. GLANDMAND SEMINAL VESICLES SKELETAL MUSCLE SPINAL CORD SPLEEN THYROID GLANDS TRACHEA URINARY BLADDER MICRO: ADRENAL CORTEX ADRENAL MEDULLA LARYNX LUNGS STOMACH, GLD STOMACH, NONGLD TRACHEA NASALLEVELI GROSS GRADE CODE: l SLIGHT. Z MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, Z MILD, 3 MODERATE, 4 SEVERE, P PRESENT TABLE 21 (SCHEDULED NECROPSY) PROJECT NO.: WIL 417001 ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS PAGE 27 SPoNSoR: HAP TASK FORCE GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS ANIMAL NO. 61439 GROUP 4: 1527 PPM MALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ___

ORGAN WEIGHT ABS. (0) BRAIN 1.78 LIVER 7.90 KIDNEYS 1.80 LUNGS 1.02 TESTES 2.32 ADRENAL GLANDS 0.0585 FINAL BODY NT( G) 172. ________________ REL. CECUM 1.035 CECUM 4.593 1.047 JEJUNUM 0.593 JEJUNUM 1.349 0.034 KIDNEYS LIVER LIVER LARYNX STOMACH STOMACH, GLD __________________~~~ GROSS: DARK RED CONTENTS P MICRO: NO SIGNIFICANT CHANGES OBSERVED NO HISTOLOGIC CHANGES TO EXPLAIN RED CONTENTS. GROSS: DARK RED CONTENTS P MICRO: NO SIGNIFICANT CHANGES OBSERVED NO HISTOLOGIC CORRELATION TO EXPLAIN DARK RED CONTENTS. MICRO: MINERALIZATION, TUBULAR 1 GROSS: DARK RED AREA( S) P SEVERAL, IRREGULARLY SHAPED, ALL LOBES MICRO: NECROSIS, HEPATOCELLULAR, CENTRILOBULAR 3 BRIDGING CENTRILOBULAR NECROSIS, INFLAMMATION, AND HEMORRHAGE THAT CORRESPONDS TO THE GROSS FINDING OF DARK RED AREAS. MICRO: INFLAMMATION, ACUTE 1 INFLAMMATION,. WLTH EXUDATION AND ASSOCIATED PLANT MATERIAL, AT AND NEAR THE BASE OF THE EPIGLOTTIS AND DISTAL LARYNX. EXUDATE, SUPPURATIVE 2 VOCAL PROCESSES AND VENTRAL POUCH WERE NOT WELL PRESENTED IN SECTION, RECUT SECTIONS WERE EXAMINED. GROSS: DARK RED CONTENTS P MICRO: EROSION 1 EROSIONS AT THE JUNCTION OF THE PYLORIS AND DUODENUM AND IS THE LIKELY CAUSE OF DARK RED CONTENTS IN THE GASTROINTESTINAL PAGE 28 PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN .RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS ANIMAL NO. 61439 GROUP 4: 1527 PPM MALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ____________________________ L__________"

URINARY BLADDER URINARY BLADDER NASAL LEVEL II NASAL LEVEL III NASALLEVEL IV NASAL LEVEL V NASAL LEVEL VI NO SIGNIFICANT CHANGES OBSERVED TRACT. ULCERATION ULCER AT THE PROXIMAL PYLORIC REGION ALSO A LIKELY CAUSE FOR DARK RED CONTENTS. GROSS: DARK RED CONTENTS MICRO: NO SIGNIFICANT CHANGES OBSERVED NO HISTOLOGIC CORRELATION TO EXPLAIN DARK RED CONTENTS. MICRO: NECROSIS, SINGLE CELL TRANSITIONAL EPITHELIUM OF TURBINATES AND LATERAL WALL. NECROSIS, RESPIRATORY EPITHELIUM NECROSIS, DORSAL MEATUS. MICRO: NECROSIS. OLFACTORY EPITHELIUM NECROSIS AND LOSS OF EPITHELIUM AND UNDERLYING GLANDS. MICRO: NECROSIS, OLFACTORY EPITHELIUM NECROSIS AND LOSS OF EPITHELIUM AND UNDERLYING GLANDS, DORSAL MSATUS AND ETHMOID TURBINATES. MICRO: NECROSIS, OLFACTORY EPITHELIUM MICRO: NOT EXAMINED SEVERELY DAMAGED PRIOR TO TRIMMING, SECTION INADEQUATE.

GROSS: ADRENAL GLANDS STERNEBRAE BRAIN COLON DUODENUM AORTA EPIDIDYMIDBS ESOPHAGUS PROJECT NO.: WIL 417001 SPONSOR:? lAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1.1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 29

ANIMAL NO. 61439 GROUP 4: 1527 PPM MALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE "_______________________________________ EYES/ OPTIC N. LYMPH NODE, TR/ BR HEART ILEUM KIDNEYS LYMPH NODE, MED NASAL TISSUE LARYNX TESTES LUNGS THYMUS GLAND NERVB, SCIATIC PANCREAS RECTUM PITUITARY PROSTATE SAL. GLAND MAND SEMINAL VESICLES SKELBTAL MUSCLE SKIN SPINAL CORD SPLEEN THYROID GLANDS TRACHEA MICRO: ADRENAL CORTEX ADRENAL MEDULLA CECUM JEJUNUM LUNGS STOMACH, NONGLD TRACHEA URINARY BLADDER NASAL LEVEL I NOT EXAMINED MICRO: NASAL LEVEL VI GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, Z MILD, 3 MODERATE, 4 SEVERE, P PRESENT PROJECT NO.: WIL 417001 TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS SPONSOR: HAP TASK FORCE GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS ANIMAL NO. 61446 GROUP 1: 0 PPM FEMALE SCHEDULED EUTH 02/ 26/ 01 _______________ L__ ORGAN WEIGHT ABS. (G) REL. LIVER MICRO: INFILTRATE. MONONUCLEAR BRAIN 1.69 1.280 NO SIGNIFICANT LIVER 5.64 4.273 CHANGES OBSERVED GROSS: ADRENAL GLANDS KIDNEYS 1.19 0.902 COLON LUNGS 0.82 0.621 EYES/ OPTIC N. OVARIES 0.0905 0.069 ILEUM ADRENAL GLANDS 0.0471 0.036 LYMPH NODE, MED FINAL BODY WT( G) 132. THYMUS GLAND RECTUM SKIN THYROID GLANDS VAGINA MICRO: ADRENAL CORTEX LUNGS NASAL LEVEL II NASAL LEVEL VI STERNEBRAE BRAIN CECUM DUODENUM AORTA ESOPHAGUS OVIDUCTS LYMPH NODE, TR/ BR HEART JEJUNtJM KIDNEYS LIVER NASAL TISSUE LARYNX LUNGS NERVE, SCIATIC OVARIES PANCREAS PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SPINAL CORD SPLEEN STOMACH TRACHEA URINARY BLADDER UTERUS PAGE 30 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GFADE __________________________________ 1 ADRENAL MEDuLLA KIDNEYS LARYNX STOMACH, GLD TRACHEA NASAL LEVEL I NASAL LEVEL III NASAL LEVEL IV NASAL LEVEL V GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, 2 MILD, 3 MODERATE, I SEVERE, P PRESENT PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATH~ LCGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 31 ANIMAL NO. 61452 GROUP ORGAN WEIGHT ABS, (G) BRAIN 1.63 LIVER 4.98 KIDNEYS 1.11 LUNGS 0.84 OVARIES 0.0816 ADRENAL GLANDS 0.0462 FINAL BODY WT( G) 130. 1: 0 PPM FEMALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ._______________________________________~~ REL. KIDNEYS MICRO: INFLAMMATION, CHRONIC 1 1.254 LIVER MICRO: INFILTRATE, MONONUCLEAR 1 3.831 NO SIGNIFICANT 0.854 CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNEBRAE BRAIN CECUM 0.646 COLON DUODENUM AORTA ESOPHAGUS 0.063 EYES/ OPTIC N, OVIDUCTS LYMPH NODE, TR/ BR HEART 0.036 ILEUM JEJUNUM KIDNEYS LIVER LYMPH NODE, MED NASAL TISSUE LARYNX LUNGS THYMUSGLAND NERVE, SCIATIC OVARIES PANCREAS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA MICRO: ADRENAL CORTEX ADRENAL MEDULLA LARYNX LUNGS STOMACH, GLD TRACHEA NASAL LEVEL I NASAL LNEL II NASAL LEVEL III NASAL LEVEL IV NASAL LEVEL V NASAL LEVEL VI GROSS GRADE CODE: l SLIGHT, 2 MODERATE, J MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, I MILD, 3 MODERATE, 4 SEVERE, P PRESENT PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 32 ANIMAL NO. 61453 GROUP 1:

__

ORGAN WEIGHT ABS. (G)

BRAIN 1.57 LIVER 4.46 KIDNEYS 1.09 LUNGS 0.77 OVARfES 0.0867 ADRENAL GLANDS 0.0461 FINAL BODY WT( G) 114. . REL. 1.377 3.912 0.956 0.675 0.076 0.040 0 PPM FEMALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE _______________* LIVER MICRO: INFILTRATE, MONONUCLEAR 1 LARYNX MICRO: NO SIGNIFICANT CHANGES OBSERVED VOCAL PROCESSES AND THE BASE OF THE EPIGLOTTIS WERE NOT WELL PRESENTED IN SECTION, RECUT SECTIONS WERE EXAMINED. NASAL LEVEL II MICRO: INFLAMMATION, SUBACUTE 1 LATERAL WALL OPPOSITE THE MAXILLOTURBINATES. NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNEBRAE BRAIN CECUM COLON DUODENUM AORTA ESOPHAGUS EYES/ OPTIC N. OVIDUCTS LYMPH NODE, TR/ BR HEART ILEUM JEJUNUM KIDNEYS LIVER LYMPH NODE, MED NASAL TISSUE LARYNX LUNGS THYMUS GLAND NERVE, SCIATIC OVARIES PANCREAS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA MICRO: ADRENAL CORTEX ADRENAL MEDULLA KIDNEYS LARYNX LUNGS STOMACH, GLD TRACHEA NASAL LEVEL I NASAL LEVEL III NASAL LEVEL IV NASAL LEVEL V NASAL LEVEL VI GROSS GRADE CODE: l SLIGHT, Z MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, Z MILD, 3 MODERATE, B SEVERE, P PRESENT PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE mm3 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 33 ANIMAL NO. 61456 GROUP ___ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS OVARIES ADRENAL GLANDS FINAL BODY WT( G) ABS. (G) 1.69 4.64 1.16 0.76 0.0721 0.0398 122. 1: 0 PPM FEMALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ____________________

REL. GENERAL COMMENT GROSS: ORGAN DAMAGED AT NECROPSY P 1.385 BRAIN 3.803 LARYNX MICRO: INFLAMMATION, ACUTE 1 0.951 ACUTE TO SUBACUTE INFLAMMATION, VENTRAL POUCH. 0.623 SPLEEN GROSS: ACCESSORY P 0.059 ONE, LESS THAN 1 MM IN DIAMETER 0.033 SPLEEN MICRO: ACCESSORY SPLEEN P CORRESPONDS TO THE GROSS FINDING OF ACCESSORY SPLEEN. NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNEBRAE BRAIN CECUM COLON DUODENUM AORTA ESOPHAGUS EYES/ OPTIC N. OVIDUCTS LYMPH NODE. TR/ ER HEART ILEUM JEJUNUM KIDNEYS LIVER LYMPH NODE, MED NASAL TISSUE LARYNX LUNGS THYMUSGLAND NERVE, SCIATIC OVARIES PANCREAS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA MICRO: ADRENAL CORTEX ADRENAL MEDULLA KIDNEYS LIVER LUNGS STOMACH, GLD TRACHEA NASAL LEVEL I NASAL LEVEL II NASAL LEVEL III NASAL LEVEL IV NASAL LEVEL V NASAL LEVEL VI GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, 2 MILD, 3 MODERATE, ~ SEVERE, P PRESENT PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 23 (SCHEDULED NECROFSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF l, l, Z TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 34 ANIMAL NO. 61491 GROUP 1: 0 PPM FEMALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 _r____________ .___

ORGAN WEIGHT ABS. (G) REL. BRAIN 1.62 1.246 LIVER 5.30 4.077 KIDNEYS 1.14 0.077 LUNGS 0.80 0.615 OVARIES 0.0894 0.069 ADRENAL GLANDS 0.0486 0.038 FINAL BODY WT( G) 130. KIDNEYS LIVER LARYNX NASAL LEVEL II NASAL LEVEL III NASAL LEVEL IV NASAL LEVEL V NASAL LEVEL VI NO SIGNIFICANT CHANGES OBSERVED GRADE ____________________________________

MICRO: NEPHROPATHY 1 MICRO: INFILTRATE, MONONUCLEAR 1 MICRO: INFLAMMATION, ACUTE 1 EDEMA 1 ACUTE TO SUBACUTE INFLAMMATION, DISTAL LARYNX, VENTRAZ FOUCH, AND NEAR THE BASE OF THE EPIGLOTTIS. THE VOCAL PROCESSES WERE NOT WELL PRESENTED IN SECTION, RECUTS WERE EXAMINED. EXUDATE, SUPPURATIVE 1 MICRO: INFLAMMATION, SUBACUTE 1 TRANSITIONAL EPITHELIUM OF TURBINATES AND LATERAt WALL. METAPLASIA, SQUAMOUS 1 MICRO: INFLAMMATION, SUBACUTE 1 INFLAMMATION, MAXILLOTURBINATE. MICRO: INFLAMMATION, SUBACUTE 2 INFLAMMATION OF THE RESPIRATORY EPITHELIUM, DORSAL LATERAL MEATUS. MICRO: INFLAMMATION, SUBACUTE 1 INFLAMMATION, RESPIRATORY EPITHELIUM. MICRO: INFLAMMATION, SUBACUTE 2 INFLAMMATION, RESPIRATORY EPITHELIUM.

GROSS: ADRENAL GLANDS STERNEBRAE BRAIN COLON DUODENUM AORTA CECUM ESOPHAGUS PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. Tax. (WITH HI~ TOPATHOLGGY) STUDY OF 1,1,2 TIE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 35 ANIMAL NO. 61491 GROUP 1: 0 PPM FEMALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE __________ p___________________________~~

EYES/ OPTIC N. OVIDUCTS LYMPH NODE, TR/ BR HEART ILEUM JEJUNUM KIDNEYS LIVER LYMPH NODE, MED NASAL TISSUE LARYNX LUNGS THYMUSGLAND NERVE, SCIATIC OVARIES PANCREAS RECTUM PITUITARY SAL. GLAND MAND SXELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA MICRO: ADRENAL CORTEX ADRENALMEDULLA LUNGS STOMACH. GLD TRACHEA NASAL LEVEL I GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, Z MILD, 3 MODERATE, 4 SEVERE, P PRESENT PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE ANIMAL NO. 61467 GROUP ___ ORGAN WEIGHT ABS. (G) BRAIN 1.61 LIVER 4.69 KIDNEYS 1.00 LUNGS 0.79 OVARIES 0.0757 TABLE 21 (SCHEDULED NECROFSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF l, l, P TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 36 2: 50 PPM FEMALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ___________________________________~

REL. LIVER MICRO: INFILTRATE, MONONUCLEAR 1 1.376 LAKYNX MICRO: NO SIGNIFICANT CHANGES OBSERVED 4.009 BASE OF THE EPIGLOTTIS NOT WELL PRESENTED IN SECTION, RECUT 0.855 SECTIONS WERE EXAMINED. 0.675 NASAL LEVEL II MICRO: INFLAMMATION, SUBACUTE 1 0.065 LATERAL WALL OPPOSITE THE MAXILLOTUKBINATES. ADRENAL GLANDS 0.0377 0.032 NASAL LEVEL IV MICRO: NECROSIS, OLFACTORY EPITHELIUM FINAL BODY MT( G) 117. NASAL LEVEL V MICRO: NECROSIS, OLFACTORY EPITHELIUM NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STEKNEBKAE COLON DUODENUM EYES/ OPTIC N. OVIDUCTS ILEUM JEJUNUM LYMPH NODE, MED NASAL TISSUE THYMUS GLAND NERVE, SCIATIC RECTUM PITUITARY SKIN SPINAL CORD THYKOID GLANDS TRACHEA VAGINA MICRO: KIDNEYS LARYNX NASAL LEVEL III NASAL LEVEL VI 1 1 BRAIN CECUM AORTA ESOPHAGUS LYMPH NODE, TR/ BK HEART KIDNEYS LIVER LARYNX LUNGS OVARIES PANCREAS SAL. GLAND MAND SKELETAL MUSCLE SPLEEN STOMACH URINARY BLADDER UTERUS STOMACH, OLD STOMACH, NONGLD GROSS GRADE CODE: l SLIGHT, Z MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, Z MILD, 3 MODERATE, 4 SEVERE, P PRESENT PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 37 ANIMAL NO. 61470 GROUP ____"____ ____

ORGAN WEIGHT ABS. (G) BRAIN 1.62 LIVER 4.14 KIDNEYS 1.01 LUNGS 0.71 OVARIES 0.0640 ADRENAL GLANDS 0.0418 FINAL BODY W'?( G) 119. 2: 58 PPM FEMALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE .______________________________________* REL. LIVER MICRO: INFILTRATE, MONONUCLEAR 1 1.361 LARYNX MICRO: NO SIGNIFICANT CHANGES OBSERVED 3.479 VOCAL PROCESSES AND THE BASE OF THE EPIGLOTTIS WERE NOT WELL 0.849 PRESENTED IN SECTION, RECUT SECTIONS WERE EXAMINED. 0.597 NASAL LEVEL IV MICRO: NECROSIS, OLFACTORY EPITHELIUM 1 0.054 NO SIGNIFICANT 0.035 CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNEBRAE BRAIN CECUM COLON DUODENUM AORTA ESOPHAGUS EYES/ OPTIC N. OVIDUCTS LYMFH NODE, TR/ BR HEART ILEUM JEJUNUM KIDNEYS LIVER LYMPH NODE, MED NASAL TISSUE LARYNX LUNGS THYMUS GLAND NERVE, SCIATIC OVARIES PANCREAS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTBRUS VAGINA MICR0: KIDNEY. Y LARYNX STOMACH, GLD STOMACH, NONGLD NASAL LEVEL II NASAL LEVEL III NASAL LEVEL V NASAL LEVEL VI GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, 2 MILD, 3 MODERATE, I SEVERE, P PRESENT PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 38 ANIMAL NO. 61472 GROUP _____________ _____

ORGAN WEIGHT ABS. (G) BRAIN 1.67 LIVER 5.27 KIDNEYS 1.21 LUNGS 0.81 OVARIES 0.0892 ADRENAL GLANDS 0.0496 FINAL BODY WT( G) 131.

l o 0 W 2: 58 PPM FEMALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ________________________________________ REL. LIVER MICRO: INFILTRATE, MONONUCLEAR 1 1.275 LARYNX MICRO: INFLAMMATION, ACUTE 1 4.023 ACUTE To SUSACUTE INFLAMMATION, VENTRAL POUCH. THE BASE OF 0.924 THE EPIGLOTTIS WAS NOT WELL PRESENTED IN SECTION, RECUT 0.618 SECTIONS WERE EXAMINED. 0.068 NASAL LEVEL II MICRO: METAPLASIA, SQUAMOUS 1 0.038 NASAL LEVEL IV MICRO: INFLAMMATION, SUBACUTE 1 INFLAMMATION OF THE RESPIRATORY EPITHELIUM, DORSAL LATERAL MBATUS. NECROSIS, OLFACTORY EPITHELIUM 2 DORSAL MEATUS, SEPTUM, AND ETHMOID TURBINATES. NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNEBRAE BRAIN CECUM COLON DUODENUM AORTA ESOPHAGUS EYES/ OPTIC N. OVIDUCTS LYMPH NODE, TR/ BR HEART ILEUM JEJUNUM KIDNEYS LIVER LYMPH NODE, MED NASAL TISSUE LARYNX LUNGS THYMUS GLAND NERVE, SCIATIC OVARIES PANCREAS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA MICRO: KIDNEYS STOMACH, GLD STOMACH, NONGLD NASAL LEVEL III NASAL LEVEL V NASAL LEVEL VI GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, I MILD, 3 MODERATE, 4 SEVERE, P PRESENT PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 39 ANIMAL NO. 61475 GROUP ________ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS OVARIES ADRENAL GLANDS FINAL BODY WT( G) ABS. (G) 1.66 5.13 1.16 0.87 0.0959 0.0571 130. 2: 58 PPM FEMALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ._______*~___~__~~~_ r~__~__~___~__________________~____ r~~_~~~ r~~_~~~~~~_____~________________ REL. LIVER MICRO: INFILTRATE, MONONUCLEAR 1 1.277 LARYNX MICRO: NO SIGNIFICANT CHANGES OBSERVED 3.346 VOCAL PROCESSES WERE NOT WELL PRESENTED IN SECTION, RECUT 0.892 SECTIONS WERE EXAMINED. 0.669 NASAL LEVEL III MICRO: INFLAMMATION, SUBACUTE 1 0.074 INFLA~ AT~~ N. DORSAL ~83~~ s (RESPIRATORY EPITHELIUM). 0.044 NASAL LEVEL IV MICRO: NECROSIS, OLFACTORY EPITHBLIUM 2 DORSAL MEATUS. NASAL LEVEL V MICRO: NECROSIS, OLFACTORY EPITHELIUM 2 NASAL LEVEL VI MICRO: INFLAMMATION, SUBACUTE 2 INFLAMMATION, RESPIRATORY EPITHELIUM. NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNEBRAE BRAIN CECUM COLON DUODENUM AORTA ESOPHAGUS EYES/ OPTIC N. OVIDUCTS LYMPH NODE, TR/ BR HEART ILEUM JEJUNUM KIDNEYS LIVER LYMPH NODE, MED NASAL TISSUE LARYNX LUNGS THYMUS GLAND NERVE, SCIATIC OVARIES PANCREAS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA MICRO: KIDNEYS LARYNX STOMACH, GLD STOMACH, NONGLD NASAL LEVEL II GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, 2 MILD, 3 MODERATE, 4 SEVERE, P PRESENT PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 40 ANIMAL NO. 61477 GROUP 2: 58 PPM FEMALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ____________________~~" ORGAN WEIGHT ABS. (G) BRAIN 1.68 LIVER 5.40 KIDNEYS 1.11 LUNGS 0.78 OVARIES 0.0892 ADRENAL GLAND. 5 0.0444 FINAL BODY WT( G) 127. REL. LARYNX 1.323 4.252 0.874 NASAL LEVEL II 0.614 0.070 NASAL LEVEL III 0.035 NASAL LEVEL IV NASAL LEVEL V NASAL LEVEL VI NO SIGNIFICANT CHANGES OBSERVED MICRO: NO SIGNIFICANT CHANGES OBSERVED VOCAL PROCESSES AND THE BASE OF THE EPIGLOTTIS WERE NOT WELL PRESENTED IN SECTION, RECUT SECTIONS WERE EXAMINED. MICRO: METAPLASIA, SQUAMOUS LATERAL WALL OPPOSITE THE MAXILLOTURBINATES. MICRO: NECROSIS, OLFACTORY EPITHELIUM MICRO: INFLAMMATION, SUBACUTE RESPIRATORY EPITHELIUM. NECROSIS, OLFACTORY EPITHELIUM MICRO: INFLAMMATION, SUBACUTE INFLAMMATION, RESPIRATORY EPITHELIUM. MICRO: INFLAMMATION, SUBACUTE ACUTE TO SUBACUTE INFLAMMATION OF THE TURBINATES (OLFACTORY EPITHELIUM).

GROSS: ADRENAL GLANDS STERNEBRAE BRAIN CECUM COLON DUODENUM AORTA ESOPHAGUS EYES/ OPTIC N. OVIDUCTS LYMPH NODE. TR/ BR HEART ILEUM JEJUNUM KIDNEYS LIVER LYMPH NODE, MED NASAL TISSUE LARYNX LUNGS THYMUS GLAND NERVE, SCIATIC OVARIES PANCREAS RECTUM PITUITARY SAL. GLAND NAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA 206 PAGE 42 PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECR~ PSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS ANIMAL NO. 61449 GROUP 3: 181 PPM FEMALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE _______________________________________ ORGAN WEIGHT ABS. (Cl BRAIN 1.57 LIVER 5.18 KIDNEYS 1.05 LUNGS 0.69 OVARIES 0.0847 ADRENAL GLANDS 0.0420 FINAL BODY WT( G) 120. REL. LIVER 1.308 4.317 0.875 0.575 0.071 LARYNX 0.035 NASAL LEVEL II NASAL LEVEL III NASAL LEVEL IV NASAL LEVEL V NO SIGNIFICANT CHANGES OBSERVED MICRO: NECROSIS, HEPATOCELLULAR, CENTRILOBULAR 1 CENTRILOBULAR NECROSIS WITH EXTENSIVE ASSOCIATED INFLAMMATION. INFLAMMATION, SUBACUTE 1 MONONUCLEAR CELL INFILTRATES TO SUBACUTE INFLAMMATION AROUND CENTRAL VEINS. MICRO: NO SIGNIFICANT CHANGES OBSERVED VOCAL PROCESSES WERE NOT WELL PRESENTED IN SECTION, RECUT SECTIONS WERE EXAMINED. MICRO: METAPLASIA, SQUAMOUS LATERAL WALL OPPOSITE THE MAXILLOTURBINATES. MICRO: NECROSIS, OLFACTORY EPITHELIUM NECROSIS AND LOSS OF EPITHELIUM AND UNDERLYING GLANDS. MICRO: NECROSIS, OLFACTORY EPITHELIUM DORSAL MEATUS, SEPTUM, AND ETHMOID TURBINATES. MICRO: NECROSIS, OLFACTORY EPITHELIUM GROSS: ADRENAL GLANDS STERNEBRAE BRAIN CECUM COLON DUODENUM AORTA ESOPHAGUS EYES/ OPTIC N. OVIDUCTS LYMPH NODE, TR/ BR HEART ILEUM JEJUNUM KIDNEYS LIVER LYMPH NODE, MED NASAL TISSUE LARYNX LUNGS THYMUS GLAND NERVE, SCIATIC OVARIES PANCREAS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPSNAL CORD SPLEEN STOMACH PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 43 ANIMAL NO. 61449 GROUP 3: 181 PPM FEMALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE _______________ I________________________~~

THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA MICRO: KIDNEYS LARYNX STOMACH, GLD STOMACH, NONGLD NASAL LEVEL VI GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, Z MILD, 3 MODERATE, 4 SEVERE, P PRESENT PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TILE 21 (SCHEDULED NEcRoPSY) ACUTE INH. TAX. (WITH HIST~ PATHOLOGY) STUDY 0~ 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 44 ANIMAL NO. 61474 GROUP 3: 181 PPM FEMALE SCHEDULED ELJTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ________________________________________________________________________.__________________

ORGAN WEIGHT ABS.( G) REL. LIVER MICRO: NECROSIS, HEPATOCELLULAR, CENTRILORULAR 1 BRAIN 1.52 1.357 CENTRILOBULAR NECROSIS WITH EXTENSIVE ASSOCIATED INFLAMMATION. LIVER 4.68 4.179 INFLAMMATION, SUBACUTE 2 KIDNEYS 1.00 0.893 MONONUCLEAR CELL INFILTRATES TO SUBACUTE INFLAMMATION AROUND LUNGS 0.69 0.616 CENTRAL VEINS. OVARIES 0.0827 0.074 NASAL LEVEL IV MICRO: NECROSIS, OLFACTORY EPITHELIUM 2 ADRENAL GLANDS 0.0450 0.040 NASAL LEVEL V MICRO: NECROSIS, OLFACTORY EPITHELIUM 2 FINAL BODY WT.( G) 112. NASAL LEVEL VI MICRO: NECROSIS, OLFACTORY EPITHELIUM 1 DIFFUSELY SCATTERED SINGLE CELL NECROSIS. NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNEBRAE BRAIN CECUM COLON DUODENUM AORTA ESOPHAGUS EYES/ O? TIC N. OVIDUCTS LYMPH NODE, TR/ BR HEART ILEUM JEJUNUM KIDNEYS LIVER LYMPH NODE, MED NASAL TISSUE LARYNX LUNGS THYMUS GLAND NERVE, SCIATIC OVARIES PANCREAS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA MICRO: KIDNEYS LARYNX STOMACH, GLD STOMACH, NONGLD NASAL LEVEL II NASAL LEVEL III GROSS GRADE CODE: l SLIGHT, Z MODERATE, 3 MARKED. P PRESENT MICRO GRADE CODE: l MINIMAL, 2 MILD, 3 MODERATE, 4SEVERE. p PRESENT PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROFSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 45 ANIMAL NO. 61482 GROUP _______   ORGAN WEIGHT ABS. (G) BRAIN 1.63 LIVER 5.44 KIDNEYS 1.12 LUNGS 0.70 OVARIES 0.0685 ADRENAL GLANDS 0.0455 FINAL BODY WT( G) 126.

E 0 3: 181 PPM FEMALE SCHEDULED EUTH 02/ 26, '01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ________________________________________~

REL. LIVER MICRO: NECROSIS, HEPATOCELLULAR, CENTRILOBULAR 1 1.294 CENTRILOBLBAR NECROSIS WITH EXTENSIVE ASSOCIATED INFLAMMATION. 4.317 INFLAMMATION, SUBACUTE 2 0.889 MOST OF THE INFLAMMATION IS SUBCAPSULAR BUT SIMILAR TO THAT IN 0.556 CENTRILOBULAR AREAS. 0.054 IARYNX MICRO: INFLAMMATION, ACUTE 1 0.036 NASAL LEVEL III MICRO: NECROSIS, OLFACTORY EPITHELIUM 2 NECROSIS AND LOSS OF EPITHELIUM AND UNDERLYING GLANDS. INFLAMMATION, SUBACUTE 1 INFLAMMATION, RESPIRATORY EPITHELIUM AND GLANDS, SEPTUM. NASAL LEVEL IV MICRO: NECROSIS, OLFACTORY EPITHELIUM 2 DORSAL MEATUS, SEPTUM, AND ETHMOID TURBINATES. NASAL LEVEL V MICRO: NECROSIS, OLFACTORY EPITHELIUM 1 WIDELY SCATTERED SINGLE CELL NECROSIS. NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNEBRAE BRAIN CECUM COLON DUODENUM AORTA ESOPHAGUS EYES/ OPTIC N. OVIDUCTS LYMPH NODE, TR/ BR HE& RT ILEUM JEJUNUM KIDNEYS LIVER LYMPH NODE, MED NASAL TlSSUE LARYNX LUNGS THYMUS GLAND NERVE, SCIATIC OVARIES PANCREAS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA 211 PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLCGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 47 ANIMAL NO. 61486 GROUP ____.__. ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS OVARIES ADRENAL GLANDS FINAL BODY WT( G) ABS.( G) 1.64 5.07 1.12 0.71 0.0816 0.0539 121. 3: 181 PPM FEMALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE .____________ REL. LIVER MICRO: INFILTRATE, MONONUCLEAR 1 1.355 LARYNX MICRO: NO SIGNIFICANT CHANGES OBSERVED 4.190 VOCAL PROCESSES AND THE BASE OF THE EPIGLOTTIS WERE NOT WELL 0.926 FRESENTED IN SECTION, RECUT SECTIONS WERE EXAMINED. 0.587 SKIN GROSS: RED MATTING P 0.067 OCULAR, RIGHT 0.045 NASAL LEVEL II MICRO: NECROSIS, SINGLE CELL 1 NECROSIS ON THE TURBINATES, PRIMARILY THE NASOTURBINATES. EXUDATE 1 NASAL LEVEL III MICRO: NECROSIS, OLFACTORY EPITHELIUM 2 NASAL LEVEL IV MICRO: NECROSIS, OLFACTORY EPITHELIUM 2 NASAL LEVEL V MICRO: NECROSIS, OLFACTORY EPITHELIUM 2 NASAL LEVEL VI MICRO: NECROSIS, OLFACTORY EPITHELIUM 1 DIFFUSELY SCATTERED SINGLE CELL NECROSIS. NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNEERAE BRAIN CECUM COLON DUODENUM AORTA ESOPHAGUS EYES/ OPTIC N. OVIDUCTS LYMPH NODE, TR/ BR HEART ILEUM JEJUNUM KIDNEYS LIVER LYMPH NODE, MED NASAL TISSUE LARYNX LUNGS THYMUS GLAND NERVE, SCIATIC OVARIES PANCREAS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SPIN& CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA MICRO: KIDNEYS LARYNX STOMACH, GLD STOMACH, NONGLD GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, F PRESENT MICRO GRADE CODE: l MINIMAL, 2 MILD, 3 MODERATE, 4 SEVERE, P PRESENT TABLE 21 (SCHEDULED NECROPSY) PROJECT NO.: WIL 417001 ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF l, l, Z TCE IN RATS PAGE 48 SPONSOR: HAP TASK FORCE GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS ANIMAL NO. 61493 GROUP 3: 181 PPM FEMALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 ________ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS OVARIES ADRENAL GLANDS FINAL BODY WTTG)        _ _ ABS. (G) 1.53 4.76 0.98 0.61 0.0616 0.0441 108. ______ L_______

REL. LIVER 1.417 4.407 LARYNX 0.907 0.565 0.057 NASAL LEVEL III 0.041 NASAL LEVEL IV GRADE ____________________ MICRO: NECROSIS, HEPATOCELLULAR, CENTRILOEIJLAR 2 CENTRILOBm NECROSIS WITH EXTENSIVE ASSOCIATED INFLAMMATION. MICRO: NO SIGNIFICANT CHANGES OBSERVED VOCAL PROCESSES WERE NOT WELL PRESENTED IN SECTION, RECUT SECTIONS WERE EXAMINED. MICRO: INFLAMMATION, SUBACUTE 1 INFLAMMATION, NASOTURBINATE. MICRO: NECROSIS, OLFACTORY EPITHELIUM 2 NECROSIS AND LOSS OF EPITHELIUM AND UNDERLYING GLANDS, DORSAL MEATUS AND ETHMOID TURBINATES. MICRO: NECROSIS, OLFACTORY EPITHELIUM 2 NECROSIS AND LOSS OF THE EPITHELIUM AND UNDERLYING GLANDS, DORSAL MEATUS AND FACING TURBINATES AND DOFAL SEPTUM. NASAL LEVEL V NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNEBRAE BRAIN CECUM COLON DUODENUM AORTA ESOPHAGUS EYES/ OPTIC N. OVIDUCTS LYMPH NODE, TR/ BR HEART ILEUM JEJUNUM KIDNEYS LIVER LYMPH NODE, MED NASAL TISSUE LARYNX LUNGS THYMUS GLAND NERVE, SCIATIC OVARIES PANCREAS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA PROJECT NG.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE SNH. TOX. (WITH HISTOPhTHOLOGY) STUDY OF l, l, Z TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 49 ANIMAL NO. 61493 GROUP 3: 181 PPM FEMALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ____________ I_____. MICRO: KIDNEYS LARYNX STOMACH, GLD STOMACH, NONGLD NASAL LEVEL II NASAL LEVEL VI GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, Z MILD, 3 MODERATE, 4 SEVERE. P PRESENT ANIMAL NO. 61476 GROUP 4: 1527 PPM FEMALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ___________________________________"

ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS OVARIES ADRENAL GLANDS FINAL BODY WT( G) ABS. (G) 1.56 4.33 1.08 0.74 0.0641 0.0513 108. REL. DUODENUM 1.444 4.009 1.000 0.685 KIDNEYS 0.059 0.048 LIVER MICRO: HYPERPLASIA, EPITHELIAL REGENERATIVE HYPERPLASIA, WITH LOSS OF VILLI, IN THE PROXIMAL DUODENUM. IN SECTION WITH THE STOMACH. INFLAMMATION. ACUTE 2 MICRO: LUMINAL DEBRIS BASOPHILIC GRANULAR MATERIAL WITHIN CORTICAL TUBULAR LUMENS. GROSS:

MICRO: PALE ALL LOBES 2 cn PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOL~ GY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 50 LIVER LARYNX SKIN GROSS:

SKIN GROSS:

STOMACH STOMACH, GLD GROSS: MICRO: MICRO: NECROSIS, HEPATOCELLULAR, CENTRILOBUIAR BRIDGING CENTRILOBULAR NECROSIS, INFLAMMATION, AND HEMORRHAGE THAT CORRESPONDS TO THE GROSS FINDING OF PALE. EDEMA INFLAMMATION. ACUTE ACUTE TO SUBACUTE INFLAMMATION, VENTRAL POUCH, VOCAL PROCESSES AND DISTAL LARYNX. THE BASE OF THE EPIGLOTTIS WAS NOT WELL PRESENTED IN SECTION, RECUTS WERE EXAMINED. YELLOW MATTING UROGENITAL RED MATTING FOREPAW. BILATERAL; OCULAR, BILATERAL; NASAL; BUCCAL DARK RED CONTENTS EROSION EROSIONS AT THE JUNCTION OF THE PYLORIS AND DUODENUM AND IS 2 2 PAGE 51 PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLCXiY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS ANIMAL NO. 61476 GROUP 4: 1527 PPM FEMALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE _____________~"

STOMACH, NONGLD NASAL LEVEL II NASAL LEVEL TII NASAL LEVEL IV NASAL LEVEL V NASAL LEVEL VI NO SIGNIFICANT CHANGES OBSERVED THE LIKELY CAUSE OF DARK RED CONTENTS IN THE GASTROINTESTINAL TRACT. MICRO: HEMORRHAGE CORRESPONDS TO THE GROSS OBSERVATION OF DARK RED CONTENTS. MICRO: NECROSIS, SINGLE CELL TRANSITIONAL EPITHELIUM OF TURBINATES AND LATERAL WALL. MICRO: NECROSIS, SINGLE CELL CONTINUED INVOLVEMENT OF TRANSITIONAL EPITHELIUM, TURBINATES AND LATERAL WALL. NECROSIS, OLFACTORY EPITHELIUM NECROSIS AND LOSS OF EPXTHELIUM AND UNDERLYING GLANDS. MICRO: NECROSIS, OLFACTORY EPITHELIUM NECROSIS AND LOSS OF EPITHELIUM AND UNDERLYING GLANDS, DORSAL MEATUS AND ETHMOID TURBINATES. MICRO: NECROSIS, OLFACTORY EPITHELIUM NECROSIS AND LOSS OF THE EPITHELIUM AND UNDERLYING GLANDS, DORSAL MBATUS AND FACING TURBINATES AND DORAL SEPTUM. MICRO: NECROSIS, OLFACTORY EPITHELIUM GROSS: ADRENAL GLANDS STERNEBRAB BRAIN CECUM COLON DUODENUM AORTA ESOPHAGUS TABLE 21 (SCHEDULED NECROPSY) PROJECT NO.: WIL 417001 ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS S!? ONSOR: HAP TASK FORCE GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 52 ANIMAL NO. 61476 GROUP 4: 1527 PPM FEMALE SCHEDULED EUTH _ 1_____.

EYES/ OPTIC N. ILEUM NASAL TISSUE NERVE, SCIATIC PITUITARY SPLEEN UTERUS MICRO: ADRENAL CORTEX NASAL LEVEL I 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 GRADE ___________________________________ OVIDUCTS LYMPH NODE, TR/ BR HEART JEJUNUN KIDNEYS LYMPH NODE, MED LARYNX LUNGS THYMUS GLAND OVARIES PANCREAS RECTUM SAL. GLAND MAND SKELETAL MUSCLE SPINAL CORD THYROID GLANDS TRACHEA URINARY BLADDER VAGINA ADRENAL MEDULLA LUNGS TRACHEA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, 2 MILD, 3 MODERATE, 4 SEVERE, P PRESENT TABLE 21 (SCHEDULED NECROPSY) PROJECT NO.: WIL 417001 ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS PAGE 53 SPONSOR: HAP TASK FORCE GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS ANIMAL NO. 61484 GROUP 4: 1527 PPM FEMALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 1 __. ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS OVARIES ADRENAL GLANDS FINAL BODY WT( G) ABS. (G) 1.63 4.63 1.12 0.67 0.0729 0.0584 103. ________ REL. ADRENAL CORTEX 1.583 LIVER 4.495 1.087 LARYNX 0.650 0.071 0.057 SKIN NASAL LEVEL II NASAL LEVEL III NASAL LEVEL IV NASAL LEVEL V GRADE .____________________________________~

MICRO: NECROSIS 1 MICRO: NECROSIS, HEPATOCELLULAR, CENTRILOBULAR 3 BRIDGING CENTRILOBULAR NECROSIS, INFLAMMATION. AND HEMORRHAGE. MICRO: INFLAMMATION. ACUTE 2 ACUTE TO SUBACUTE INFLAMMATION OF THE VOCAL PROCESSES, BASE OF THE EPIGLOTTIS, AND VENTRAL POUCH. GROSS: RED MATTING P OCULAR, BILATERAL; BUCCAL MICRO: NECROSIS, SINGLE CELL 2 TRANSITIONAL EPITHELIUM OF TURBINATES AND LnTERAL WALL. MICRO: NECROSIS, OLFACTORY EPITHELIUM 4 NECROSIS AND LOSS OF EPITHELIUM AND UNDERLYING GLANDS. NECROSIS, SINGLE CELL 3 CONTINUED INVOLVEMENT OF TRANSITIONAL EPITHELIUM, TURBINATES AND LATERAL WALL. MICRO: NECROSIS, OLFACTORY EPITHELIUM 3 NECROSIS AND LOSS OF EPITHELIUM AND UNDERLYING GLANDS, DORSAL MEATUS AND ETHMOID TURBINATES. MICRO: NECROSIS, OLFACTORY EPITHELIUM 2 NECROSIS AND LOSS OF THE EPITHELIUM AND UNDERLYING GLANDS, DORSAL MEATUS AND FACING TURBINATES AND DORAL SEPTUM. PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 21 (SCHEDULED NECROPSY) ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS GROSS AND MICROSCOPIC DESCRIPTION OF ORGANS PAGE 54 ANIMAL NO. 61484 GROUP 4: 1527 PPM FEMALE SCHEDULED EUTH 02/ 26/ 01 DATE OF DEATH: 02/ 26/ 01 STUDY DAY: 3 GRADE __ I__________________________ I________

NASAL LEVEL VI MICRO: NECROSIS, OLFACTORY EPITHELIUM 2 DIFFUSELY SCATTERED SINGLE CELL NECROSIS. NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNEBRAE BRAIN CECUM COLON DUODENUM AORTA ESOPHAGUS EYES/ OPTIC N. OVIDUCTS LYMPH NODE, TR/ BR HEART ILEUM JEJUNUM KIDNEYS LIVER LYMPH NODE, MED NASAL TISSUE LARYNX LUNGS THYMUSGLAND NERVE, SCIATIC OVARIES PANCREAS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA MICRO: ADRENAL MEDULLA KIDNEYS LUNGS STOMACH, GLD TRACHEA NASAL LEVEL I GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT MICRO GRADE CODE: l MINIMAL, 2 MILD, 3 MODERATE, I SEVERE, P PRESENT PGRHv4.28 10/ 29/ 2001 PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 22 ACUTE INH. TOX. (WITH HI~ TOPATHOL~ GY) STUDY 0~ 1,1,2 TIE IN RATS ORGAN WEIGHTS AND FINAL BODY WEIGHTS (GRAMS) PAGE 1 DAY 1 MALE GROUP: 0 PPM __ L____________________________________.

ADRENAL ANIMAL FEW( G) BRAIN LIVER KIDNEYS LUNGS TESTES GLANDS ____________________________________________________~~~~~~~

61409 163. 1.67 7.56 1.36 0.90 2.02 0.0388 61410 173. 1.71 7.27 1.46 1.03 1.70 0.0370 61419 177. 1.66 8.12 1.56 0.89 1.82 0.0398 61432 191. 1.67 7.83 1.57 0.89 2.26 0.0318 61434 199. 1.83 8.84 1.62 1.11 2.20 0.0405 MEAN 181. 1.71 7.92 1.51 0.96 2.00 0.0376 S. D. 14.4 0.071 0.601 0.104 0.101 0.240 0.00349 N 5 5 5 5 5 5 5 FBW = FINAL BODY WEIGHT PROJECT NO. :w1L 417001 SPQNSDR: HAP TASK FORCE TABLE 22 ACUTE INH. TOX. (WITH HISTOPATHOLGGYI STUDY OF 1,1,2 TCE IN RATS ORGAN WEIGHTS AND FINAL BODY WEIGHTS (GRAMS) PAGE 2 DAY 1 MALE GROUP: 58 PPM _______________________________________

ADRENAL ANIMAL FEW( G) BRAIN LIVER KIDNEYS LUNGS TESTES GLANDS ___________________

61396 180. 1.69 7.12 1.38 0.89 2.09 0.0356 61405 178. 1.72 8.01 1.47 0.96 1.95 0.0370 61413 160. 1.67 6.62 1.27 0.84 1.79 0.0268 61416 197. 1.77 8.89 1.75 1.00 2.32 0.0435 61431 169. 1.74 7.62 1.50 0.95 1.48 0.0427 MEAN 177. 1.72 7.65 1.47 0.93 1.93 0.0371 S. D. 13.8 0.040 0.867 0.178 0.063 0.316 0.00672 N 5 5 5 5 5 5 5 FEW = FINAL BODY WEIGHT PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 22 ACUTE INH. TOX. (WITH HISTOPATHOLCGY) STUDY OF 1.1,2 TCE IN RATS ORGAN WEIGHTS AND FINAL BODY WEIGHTS (GRAMS) PAGE 3 DAY 1 MALE GROUP: 181 PPM ________________________________________~"*

ADRENAL ANIMAL FBW( G) BRAIN LIVER KIDNEYS LUNGS TESTES GLANDS ____ E______ s__________________________~~~~~~

61399 165. 1.74 6.29 1.38 0.84 2.11 0.0399 61411 172. 1.68 6.70 1.43 0.88 2.02 0.0405 61441 177. 1.71 8.01 1.51 0.96 2.13 0.0436 61442 178. 1.79 7.59 1.54 0.98 2.36 0.0474 61443 167. 1.69 7.20 1.42 0.85 1.96 0.0442 MEAN 172. 1.72 7.19 1.46 0.90 2.12 0.0431 S. D. 5.8 0.044 0.674 0.067 0.064 0.153 0.00304 N 5 5 5 5 5 5 5 FBW = FINAL BODY WEIGHT TABLE 22 PROJECT NO.: WIL 417001 ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF l., 1,2 TCE IN RATS PAGE 4 SPONSOR: UAP TASK FORCE ORGAN WEIGHTS AND FINAL BODY WEIGHTS (GRAMS) DAY 1 MALE GROUP: 1527 PPM ____________________~

ANIMAL FBW( G)

61397 158. 61406 152. 61408 166. 61421 144. 61439 172. BRAIN __"_________ 1.72 1.59 1.74 1.67 '1.78  ADRENAL GLANDS LIVER KIDNEYS LUNGS TESTES  _________________________________ c_~ 7.37 1.56 0.90 1.54 7.11 1.37 0.78 1.86 8.37 1.77 1.07 2.13 6.30 1.44 0.83 1.75 7.90 1.80 1.02 2.32 0.0619 0.0469 0.0641 0.0475 0.0585 MEAN 158. 1.70 7.41 1.59 0.92 1.92 0.0558 S. D. 11.1 0.073 0.788 0.193 0.123 0.309 0.00809 N 5 5 5 5 5 5 5 FBW 5 FINAL BODY WEIGHT TABLE 22 PROJECT NO.: WIL 417001 ACUTE INH. TOX. (WITH HISTOPATHOLCGY) STUDY OF 1,1,2 TCE IN RATS SPONSOR: HAP TASK FORCE PAGE 5 ORGAN WEIGHTS AND FINAL BODY WEIGHTS (GRAMS) DAY 1 FEMALE GROUP: 0 PPM __________ sL____________________________________________________________________________~~.

ANIMAL FBW( G) BRAIN ADRENAL LIVER KIDNEYS LUNGS OVARIES GLANDS _______________""~

61446 132. 1.69 5.64 1.19 0.82 61452 130. 0.0905 1.63 0.0471 4.98 1.11 0.84 61453 114. 0.0816 1.57 0.0462 4.46 1.09 0.77 61456 122. 0.0867 1.69 0.0461 4.64 1.16 0.76 61491 130. 0.0721 1.62 0.0398 5.30 1.14 0.80 0.0894 0.0488 MEAN 126. 1.64 5.00 1.14 S. D. 0.80 7.5 0.0841 0.051 0.480 0.0456 0.040 0.033 N 0.00752 5 5 5 5 0.00342 5 5 5 FBW = FINAL BODY WEIGHT PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 22 ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS ORGAN WEIGHTS AND FINAL BODY WEIGHTS (GRAMS)

FEMALE GROUP: SE Pm PAGE 6 DAY 1 ADRENAL ANIMAL FBW( G) BRAIN LIVER KIDNEYS LUNGS OVARIES GLANDS "_____________

61467 117. 1.61 4.69 1.00 0.79 0.0757 0.0377 61470 119. 1.62 4.14 1.01 0.71 0.0640 O. OGlEl 61472 131. 1.67 5.27 1.21 0.81 0 * 0892 0.0496 61475 130. 1.66 5.13 1.16 0.87 0.0959 0.0571 61477 127. 1.68 5.40 1.11 0.78 0.0892 0.0444 MEAN 125. 1.65 4.93 1.10 0.79 0.0828 0.0461 S. D. 6.4 0.031 0.514 0.092 0.058 0.01282 0.00750 N 5 5 5 5 5 5 5 FBW = FINAL BODY WEIGHT K cn PROJECT NO.: WIL 417001 SPONSOR: HAP TASK FORCE TABLE 22 ACUTE INH. TAX. (WITH HISTOPATHOLOGY) STUDY 0~ 1,1,2 TCE IN RATS ORGAN WEIGHTS AND FINAL BODY WEIGHTS (GRAMS) PAGE 7 DAY 1 FEMALE GROUP: 181 PPM _______________________________________~

ADRENAL ANIMAL FBW( G) BRAIN LIVER KIDNEYS LUNGS OVARIES GLANDS ______________________________________~~

61449 120. 1.57 5.18 1.05 0.69 0.0847 0.0420 61474 112. 1.52 4.68 1.00 0.69 0.0827 0.0450 61482 126. 1.63 5.44 1.12 0.70 0.0685 0.0455 61486 121. 1.64 5.07 1.12 0.71 0.0816 0.0539 61493 108. 1.53 4.76 0.98 0.61 0.0616 0.0441 MEAN 117. 1.58 5.03 1.05 0.68 0.0758 0.0461 S. D. 7.3 0.055 0.311 0.065 0.040 0.01019 0.00456 N 5 5 5 5 5 5 5 FBW = FINAL BODY WEIGHT TABLE 22 PROJECT NO.: WIL 417001 ACUTE INH. TOX. (WITH HISTOPATHOLCGY) STUDY OF 1,1,2 TCE IN RATS PAGE 0 SEQNSOR: HAP TASK FORCE ORGAN WEIGHTS AND FINAL BODY WEIGHTS (GRAMS) DAY 1 FEMALE GROUP: 1527 PPM ________________________________________~ ADRENAL ANIMAL FBW( G) BRAIN LIVER KIDNEYS LUNGS OVARIES GLRNDS ___________________________________^____"

61476 108. 1.56 4.33 1.08 0.74 0.0641 0.0513 61484 103. 1.63 4.63 1.12 0.67 0.0729 0.0584 MEAN 106. 1.60 4.48 1.10 0.71 0.0685 0.0549 S. D. 3.5 0.049 0.212 0.028 0.049 0.00622 0.00502 N 2 2 2 2 2 2 2 FBW = FINAL BODY WEIGHT POFBWv4.05 06/ 12/ 2001 TABLE 23 PROJECT NO.: WIL 417001 ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY 0~ 1,1,2 TCE IN RATS PAGE 1 SPONSOR: HAP TASK FORCE ORGAN WEIGHTS RELATIVE TO FINAL BODY WEIGHTS (GRAMS/ 100 GRAMS) DAY 1 MALE GROUP: 0 PPM _______________________________________ 1"~~~.~~~~~

ADRENAL ANIMAL FBW( G) BRAIN LIVER KIDNEYS LUNGS TESTES GLANDS ________________________________________~

61409 163. 1.025 4.638 0.834 0.552 1.239 0.024 61410 173. 0.988 4.202 0.844 0.595 0.983 0.021 61419 171. 0.938 4.588 0.881 0.503 1.028 0.022 61432 191. 0.874 4.099 0.822 0.466 1.183 0.017 61434 199. 0.920 4.442 0.814 0.558 1.106 0.020 MEAN 181. 0.949 4.394 0.839 0.535 1.108 0.021 S. D. 14.4 0.0589 0.2363 0.0261 0.0505 0.1058 0.0026 N 5 5 5 5 5 5 5 FBW = FINAL BODY WEIGHT TABLE 23 PROJECT NO.: WIL 417001 ACUTE INH. TOX. (WITH HISTOPATH~ L~ GY) STUDY OF 1,1.2 TCE IN RATS PAGE 2 SP0NSOR: HAP TASK FORCE ORGAN WEIGHTS RELATIVE TO FINAL BODY WEIGHTS (GRAMS/ 100 GRAMS) DAY 1 MALE GROUP: 58 PPM ______ ________________________________________

ADRENAL ANIMAL FBW( G) BRAIN LIVER KIDNEYS _________________________________________________________.._________________.________ LUNGs____________~~~~~~~~~~ 61396 180. 0.939 3.956 0.767 0.494 1.161 0.020 61405 178. 0.966 4.500 0.826 0.539 1.096 0.021 61413 160. 1.044 4.138 0.794 0.525 1.119 0.017 61416 197. 0.898 4.513 0.888 0.508 1.178 0.022 61431 169. 1.030 4.509 0.868 0.562 0.876 0.025 MEAN 177. 0.975 4.323 0.833 0.526 1.086 0.021 S. D. 13.8 0.0614 0.2603 0.0547 0.0265 0.1218 0.0029 N 5 5 5 5 5 5 5 FBW = FINAL BODY WEIGHT TABLE 23 PROJECT NO.: WIL 417'001 ACUTE INH. TOX. (NITH HXSTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS PAGE 3 SPONSOR: HAP TASK FORCE ORGAN WEIGHTS RELATIVE TO FINAL BODY WEIGHTS (GRAMS/ 100 GRAMS) DAY 1 MALE GROUP: 181 PPM ________________________________________~~ ADRENAL ANIMAL FBW( G) BRAIN LIVER KIDNEYS LUNGS TESTES GLANDS ________________________________________

61399 165. 1.055 3.812 0.836 0.509 1.279 0.024 61411 172. 0.977 3.942 0.831 0.512 1.174 0.024 61441 177. 0.966 4.525 0.853 0.542 1.203 0.025 61442 178. 1.006 4.264 0.865 0.551 1.326 0.027 61443 167. 1.012 4.359 0.850 0.509 1.174 0.026 MEAN 172. 1.003 4.180 0.847 0.525 1.231 0.025 S. D. 5.8 0.0348 0.2959 0.0137 0.0203 0.0682 0.0013 N 5 5 5 5 5 5 5 FBW = FINAL BODY WEIGHT TABLE 23 PROJECT NO.: WIL 417001 ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS PAGE 4 SPONSOR: HAP TASK FORCE ORGAN WEIGHTS RELATIVE TO FINAL BODY WEIGHTS (GRAMS/ 100 GRAMS) DAY 1 MALE GROUP: 1527 PFM ________________________________________~ ADRENAL ANIMAL FBW( GJ BRAIN LIVER KIDNEYS LUNGS TESTES GLANDS ________________________________________

61397 158. 1.099 4.665 0.987 0.570 0.975 0.039 61406 152. 1.046 4.678 0.901 0.513 1.224 0.031 61408 166. 1.048 5.042 1.066 0.645 1.283 0.039 61421 144. 1.160 4.37s 1.000 0.576 1.215 0.033 61439 172. 1.035 4.593 1.047 0.593 1.349 0.034 MEAN 158. 1.076 4.671 1.000 0.579 1.209 0.035 S. D. 11.1 0.0515 0.2405 0.0643 0.0474 0.1415 0.0036 N S S S 5 5 5 5 FBW = FINAL BODY WEIGHT TABLE 23 PROJECT NO.: WIL 417001 ACUTE INK. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS PAGE 5 SPONSOR: HAP TASK FORCE ORGAN WEIGHTS RELATIVE TO FINAL BODY WEIGHTS (GRAMS/ 100 GRAMS) DAY 1 FEMALE GROUP: 0 PPM ________________________________________

ADRENAL ANIMAL FBW( G) BRAIN LIVER KIDNEYS LUNGS OVARIES GLANDS ________________________________________

61446 132. 1.280 4.273 0.902 0.621 0.069 0.036 61452 130. 1.254 3.831 0.854 0.646 0.063 0.036 61453 114. 1.377 3.912 0.956 0.675 0.076 0.040 61456 122. 1.385 3.803 0.951 0.623 0.059 0.033 61491 130. 1.246 4.077 0.877 0.615 0.069 0.038 MEAN 126. 1.308 3.979 0.908 0.636 0.067 0.037 S. D. 7.5 0.0675 0.1958 0.0449 0.0248 0.0065 0.0026 N 5 5 5 5 5 5 5 FBW = FINAL BODY WEIGHT TABLE 23 PROJECT NO.: WIL 417001 ACUTE INH. TOX. (WITH HISTOPATHOLGGY) STUDY OF 1,1,2 TCE IN RATS PAGE 6 SPONSOR: HAP TASK FORCE ORGAN WEIGHTS RELATIVE TO FINAL BODY WEIGHTS (GRAMS/ 100 GRAMS) DAY 1 FEMALE GROUP: 58 PPM ________________________________________"~~~~~

ANIMAL FEW( G) BRAIN LIVER ___________________ ._________________ .____

61467 117. 1.376 4.009 61470 119. 1.361 3.479 61472 131. 1.275 4.023 61475 130. 1.277 3.946 61477 127. 1.323 4.252 KIDNEYS ____" 0.855 0.849 0.924 0.892 0.874 , LUNGS ___ 0.675 0.597 0.618 0.669 0.614 OVARIES __ _ _ _ 0.065 0.054 0.068 0.074 0.070 ADRENAL GLANDS  0.032 0.035 0.038 0.044 0.035 MEAN 125. S. D. 6.4 N 5 FBW = FINAL BODY WEIGHT 1.322 3.942 0.879 0.635 0.066 0.037 0.0466 0.2835 0.0304 0.0351 0.0076 0.0045 5 5 5 5 5 5 TABLE 23 PROJECT NO.: WIL 417001 ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS PAGE 7 SPONSOR: HAP TASK FORCE ORGAN WEIGHTS RELATIVE TO FINAL BODY WEIGHTS (GRAMS/ 100 GRAMS) DAY 1 FEMALE GROUP: 161 PPM ________________________________________

ADRENAL ANIMAL FBWtG) BRAIN LIVER KIDNEYS LUNGS OVARIES GLANDS ____________________"

61449 120. 1.308 4.317 0.875 0.575 0.071 0.035 61474 112. 1.357 4.179 0.893 0.616 0.074 0.040 61482 126. 1.294 4.317 0.889 0.556 0.054 0.036 61486 121. 1.355 4.190 0.926 0.587 0.067 0.045 61493 108. 1.417 4.407 0.907 0.565 0.057 0.041 MEAN 117. 1.346 4.282 0.898 0.580 0.065 0.039 S. D. 7.3 0.0485 0.0964 0.0194 0.0233 0.0087 0.0040 N 5 5 5 5 5 5 5 FEW = FINAL BODY WEIGHT PROJECT NO.: WIL 417001 Sl= 'ONSOR: :HAP TASK FORCE TABLE 23 ACUTE INH. TOX. (WITH HIST~ PATH~ LOGY) STUDY OF 1,1,2 Tc~ IN RATS ORGAN WEIGHTS RELATIVE TO FINAL BODY WEIGHTS (GRAMS/ 100 GRAMS) PAGE 8 DAY 1 FEMALE GROUP: 1527 PPM _________________. ADRENAL ANIMAL FBW( G) BRAIN LIVER KIDNEYS LUNGS OVARIES GLANDS ___________________~ 61476 108 * 1.444 4.009 1.000 0.685 0.059 0.048 61484 103. 1.583 4.495 1.087 0.650 0.071 0.057 MEAN 106. 1.514 4.252 1.044 0.668 0.065 0.053 S. D. 3.5 0.0983 0.3437 0.0615 0.0247 0.0085 0.0064 N 2 2 2 2 2 2 2 FBW = FINAL BODY WEIGHT POFBWv4.05 06/ 12/ 2001 WIL 417001 Hazardous Air Pollutant Task Force Acute Inhalation Toxicity (With Histopathology) Study of 1,1.2 Trichloroethane (1,1.2 TCE) in Rats APPENDIX A Certificate of Analvsis (Sponsor Provided Data)

236 Cc z r. i fLn2at. e 533272 %l. l?: 1I/ lc/ zc~ o s'j In Copy 'l? h@ OUW Chemical Company Cwrriif icir I: c of Analysis Pwgu 1 tu* r. D. 0. : Divy NOKC: 66433131 10 Mar w ini: 1. L. 2. `~` R~ cXILORO~ TII~ WE (STABITII ZED) SPCC: 0008'1429 9 c! ust n1. t: Vchicln: OVN'P' Ship frm: THE lmv CHwllCAL CmMPIIINY Y~ PCIRT TX 71Ni'I'ED RTA'l'YB This eul6crial mnCeCS i. tlo rcquimuncntc of Lhc sgnuiticatiw2.

RwRUltS Limits IlniK~ OK06019300 Mimi wum Murimtm . a . em_ . me . mm.. .. em,.  em_  e m,  s.

AXSay 3 wl. '99.560 99.0  mm Color 9  a 20 APHA nd ter wm 93  LSU AcicfScy (as HC:) YB" ' 1 50 Non volacils Rttsiduc Pm 1 e m 2u 237 WIL 417001 Hazardous Air Pollutant Task Force Acute Inhalation Toxicity (With Histopathology) Studv of 1.1.2 Trichloroethane (1.1.2 TCE) in Rats APPENDIX B Serological Screeniw (BioReliance Cornoration)

238 Quality Assurance Statement Study Title: RAT LEVEL II STUDY Study Number: MVZ172. WIL Principal Investigator: Kathleen M. Vincent This study has been divided into a series of in process phases. Using a random sampling approach, Quality Assurance monitors each of these phases over a series of studies. Procedures. documentation, equipment records, etc.. are examined in order to assure that the study is performed in accordance with the U. S. FDA Good Laboratory Practice Regulations (21 CFR SS), the U. S. EPA GLPs (40 CFR 792 and 40 CFR 160). the UK GLP Regulations, the Japanese GLP Standard, and the OECD Principles of Good Laboratory Practice and to assure that the study is conducted according to the protocol and reievant Standard Operating Procedures.

The following are the inspection dates, phases inspected, and report dates of QA inspections of this study.

Inspect On 23 Feb 01  23 Feb 01 To Prin Invst 23 Feb 01 To Mgmt 02 Mar 01 Phase Addition of conjugate to wells inspect On Phase 2% Feb 01  2% Feb 0 1 To Prin Invst 0 1 Mar O1 To Mgmt 02 Mar 01 Final Report This report describes the methods and procedures used in the study and the reported results accurately reflect the raw data of the study.

EIahe Siadatpour, B. S. QUALITY ASSURANCE Hbc& 2 At A DATE Page 3 241 wL417001 Hazardous Air Pollutant Task Force Acute Inhalation Toxicity (With Histopathology) Study of I, 1,2 Trichloroethane (1,1,2 TCE) in Rats APPENDIX C Pretest Clinical Observations 242 243 PAGE 2 PROJECT NO.: WIL 417001P SPONSOR: HAP TASK FORCE ACUTE INH. TOX. (WITH HISTOPATHOLOGY) STUDY OF 1,1,2 TCE IN RATS SUMMARY OF CLINICAL FINDINGS: TOTAL OCCURRENCE/ NO. OF ANIMALS ____ F E i%, A L E ____

NORMAL NO SIGNIFICANT CLINICAL OBSERVATIONS 91/ 49 DISPOSITION SENT TO NECROPSY FOR SCHEDULED PATHOLOGY EVALUATION ASSIGNED TO WIL 417001 5/ 5 40/ 40 BODY/ INTEGUMENT SCABBING DORSAL HEAD 11 1 EYES/ EARS/ NOSE ABSENCE OF RIGHT EYE 11 1 DRIED RED MATERIAL AROUND LEFT EYE 11 1 DRIED RED MATERIAL AROUND RIGHT EYE 1/ 1 b HAIR LOSS AROUND RIGHT EYE 11 1 __~~ l PRETEST PCSUV4.0 06/ l. 3/ 2001 WIL 417001 Hazardous Air Pollutant Task Force Acute Inhalation Toxicity (With Histopathology) Study of 1,1,2 Trichloroethane (1,1,2 TCE) in Rats APPENDIX D Clinical Pathology Methods, Procedures and References 245 wIL 417001 Hazardous Air Pollutant Task Force CLINICAL PATHOLOGY METHODS, PROCEDURES AND REFERENCES Serum Chemistry  Hitachi 911 Total Protein  Endpoint biuret method that utilizes a sample blank. Hitachi 911 Application Code 166. Roche Reagent, catalog number 1040901.

Total White Blood Cell Count Mix equal volumes of cell suspension and 0.4% neutral red stain (prepared in 0.9% NaCl) (for example, use 100 p. L of each). Cap tube and incubate at approximately 37° C for IO 15 minutes.

Perform hemacytometer count (normally 10 squares, 5 squares on each side, are counted). Calculate the number of cells per ti Average no. of cells per square x lo4 x 2 (2 is for staining dilution).

246 WIL 41700 1 Hazardous Air PoIlutant Task Force Acute Inhalation Toxicity (With Histopathology) Studv of 1,1,2 Trichloroethane (1 ,I .2 TCE) in Rats APPENDIX E Bronchoalveolar Lavage Cytology Report iGail L. Walter, D. V. M., D. A. C. V. P.. D. A. B. T., Clinical Pathology Consultant)

The following points should be noted for Appendix E:

1. Due to the unscheduled death of one Group 4 female, there were not always five animals/ sex/ group evaluated. Therefore, on page 1 of this cytology report, the sixth sentence of the first paragraph does not accurately reflect the number of animals evaluated for Group 4..

2. Although it cannot be confirmed at this time, it appears that for male no. 61429, a total of 201 cells were identified. Therefore, on page 1 of this cytology report, the final sentence of the first paragraph may not be correct for this animal.

These items listed above did not adversely affect the interpretation of the results for this study.

247 Clinical Pathology Report M/ IL Study Number: 417001 Bronchoalveolar Lavage Cytology Title: Acute inhalation Toxicity (with Histopathology) Study of 1,1,2 Trichloroethane (1,1,2 TCE) in Rats Introduction This study was performed at WIL Research Laboratories, Inc., 1407 George Road, Ashland. OH 448059281. The objective of this study was to characterize the toxicologic response (including the exposure levei response relationships) of the rat following a single acute inhalation exposure by evaluating gross pathology and histopathology, as well as effects of body weight, clinical condition and mortality. To characterize potential adverse effects on the respiratory tract, bktopathological examination of fixed tissues and evaluation of the cellular and biochemicai parameters in bronchoaiveolar lavage fluid (BALF) was performed. This report addresses evaluation of the cehular component of BALF. Fischer 344 CDF @( F 344) Kri: BR rats were exposed to 0, 50,200 or 1500 ppm of I ,I ,2 TCE for 4 hours. Approximately 24 hours following completion of the exposure, bronchoalveolar kvage was performed and BALF was collected from 5 ratskex/ group. Total cell counts were performed and an aliquot was used for preparation of slides for difkrential cell counts. Differential counts were performed by Dr. Gail L. Walter by visual identification of 200 nucleated cells peranimal.

Bronchoalveolar Lavage Cytology The BALF slides were well prepared and adequate for evaluation There were no alterations in BALF total cell counts or difkentiai cell counts that were considered tu be due to test article exposure. The majority of cells present were large mononuclear cells, with a small number of Lymphocytes also present in most samples. One  2 neutrophils were present in 4 of the rats in the 1500 ppm group. However, one neutrophil was also noted in one female tiom the control and 50 ppm groups. therefore these low numbers of neutrophils in the high dose group were not clearly related to test article adminktration A large number of red blood WIL 417001 I 248 cells (RBC) was present in one male in dosed at 1500 ppm. Because RBC's were not seen in large numbers in any other samples. this was considered to be incidental and not clearly related to test article administration.

Gd L. Walter, DVM Diplomate, American College of Veterinary Pathologists Diplomate. American Board of Toxicology Clinical Pathology Consultant Date W IL 417001 249 2 250 251 Appendix D (Table 1) WIL 417001 Acute Inhalation Toxicity (With Histopathology) Study of 1 ,I ,2 Trichloroethane (1,1,2 TCE) in Rats Sponsor: HAP Task Force Bronchoalveolar Lavage Fluid Leukocyte Differential Count (%)  Summary of Means MALES Group No. Large Mononuclear Cells (%) Lymphocytes Neutrophils (%) % Eosinophils (%) Epithelial Cells (%) Mitotic Figures (%)

1 MEAN 98.8 0.8 0.0 0.0 0.4 0.0 SD 1.15 0.57 0.00 0.00 0.89 0.00 N 5 5 5 5 5 5 2 MEAN 97.0 3.0 0.0 0.0 0.0 0.0 SD 5.2 5.2 0.0 0.0 0.0 0.0 N 3 3 3 3 3 3 3 MEAN 98.6 0.9 0.0 0.0 0.4 0.1 SD 1.11 0.85 0.00 0.00 0.48 0.25 N 4 4 4 4 4 4 4 MEAN 97.5 0.6 0.4 0.1 1.1 0.3 SD 2.03 0.65 0.55 0.22 1.75 0.67 N 5 5 5 5 5 5 1 Appendix D (Table 1) WIL 417001 Acute Inhalation Toxicity (With Histopathology) Study of 1 ,l ,PTrichloroethane (1 ,1,2 TCE) in Rats Sponsor: HAP Task Force Bronchoalveolar Lavage Fluid Leukocyte Differential Count (%)  Summary of Means FEMALES Group No. Large Mononuclear Cells (%) Lymphocytes Neutrophils (%) (%) Eosinophils (%) Epithelial Cells (?/) Mitotic Figures (%)

1 MEAN 99.0 0.9 0.1 0.0 0.0 0.0 SD 0.94 0.74 0.22 0.00 0.00 0.00 N 5 5 5 5 5 5 2 MEAN 98.5 0.6 0.1 0.0 0.8 0.0 SD 1.70 0.42 0.22 0.00 1.79 0.00 N 5 5 5 5 5 5 3 MEAN 96.4 1.7 0.0 0.0 1.8 0.1 SD 2.07 0.45 0.00 0.00 1.96 0.22 N 5 5 5 5 5 5 4 MEAN 96.6 2.4 0.4 0.0 0.5 0.1 SD 2.93 2.14 0.48 0.00 1 .oo 0.25 N 4 4 4 4 4 4 2 Appendix D (Table 2) WIL 417001 Acute Inhalation Toxicity (With Histopathology) Study of 1 ,1,2 Trichloroethane (1 ,I ,2 TCE) in Rats Sponsor: HAP Task Force Individual Bronchoalveolar Lavage Fluid Leukocyte Differential Count (%)

MALES Group No. Animal No. Large Mononuclear Cells (%) Lymphocytes % Neutrophils % Eosinophils (%) Epithelial Cells (%) Mitotic Figures (%)

1 61400 97.0 1 .o 0.0 0.0 2.0 0.0 61403 99.5 0.5 0.0 0.0 0.0 0.0 61404 100.0 0.0 0.0 0.0 0.0 0.0 61414 99.0 1 .o 0.0 0.0 0.0 0.0 61420 98.5 1.5 0.0 0.0 0.0 0.0 MEAN 98.8 0.8 0.0 0.0 0.4 0.0 SD 1.15 0.57 0.00 0.00 0.89 0.00 N 5 5 5 5 5 5 2 61398 100.0 0.0 0.0 0.0 0.0 0.0 61425 100.0 0.0 0.0 0.0 0.0 0.0 61435 91 .O 9.0 0.0 0.0 0.0 0.0 MEAN 97.0 3.0 0.0 0.0 0.0 0.0 SD 5.20 5.20 0.00 0.00 0.00 0.00 N 3 3 3 3 3 3 3 Appendix D (Table 2) WIL 417001 Acute Inhalation Toxicity (With Histopathology) Study of 1 ,1,2 Trichloroethane (1 ,1,2 TCE) in Rats Sponsor: HAP Task Force Individual Bronchoalveolar Fluid Leukocyte Differential Count (%)

MALES Group Animal Large Mononuclear Epithelial Cells Mitotic Figures No. Sex No. Cells (%) Lymphocytes (%) Neutrophils (%) Eosinophils (%) W 04 3 M 61407 100.0 0.0 0.0 0.0 0.0 0.0 M 61422 98.0 0.5 0.0 0.0 1.0 0.5 M 61424 97.5 2.0 0.0 0.0 0.5 0.0 M 61433 99.0 1.0 0.0 0.0 0.0 0.0 MEAN 98.6 0.9 0.0 0.0 0.4 0.1 SD 1.11 0.85 0.00 0.00 0.48 0.25 N 4 4 4 4 4 4 4 M 61418 98.5 0.5 1 .o 0.0 0.0 0.0 M 61423 100.0 0.0 0.0 0.0 0.0 0.0 M 61428 94.5 1.5 0.0 0.0 4.0 0.0 M 61429 97.0 1.0 0.0 0.5 0.0 1.5 M 61436 97.5 0.0 1.0 0.0 1.5 0.0 MEAN 97.5 0.6 0.4 0.1 1.1 0.3 SD 2.03 0.65 0.55 0.22 1.75 0.67 N 5 5 5 5 5 5 4 Appendix D (Table 2) WIL 417001 Acute Inhalation Toxicity (With Histopathology) Study of 1 ,I ,P Trichloroethane (1 ,I ,2 TCE) in Rats Sponsor: HAP Task Force Individual Bronchoalveolar Lavage Fluid Leukocyte Differential Count (%)

FEMALES Group No.

1 Animal No.

61450 61464 61469 61490 61494 Large Mononuclear Cells (%)

99.0 97.5 99.5 99.0 100.0 Lymphocytes Neutrophils Eosinophils Epithelial % (70) (33) Cells (" 3~)

1.0 0.0 0.0 0.0 2.0 0.5 0.0 0.0 0.5 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Mitotic Figures (%)

0.0 0.0 0.0 0.0 0.0 MEAN 99.0 0.9 0.1 0.0 0.0 0.0 SD 0.94 0.74 0.22 0.00 0.00 0.00 cn N 5 5 5 5 5 5 2 61454 99.5 0.5 0.0 0.0 0.0 0.0 61457 99.0 1.0 0.0 0.0 0.0 0.0 61466 99.5 0.0 0.5 0.0 0.0 0.0 61487 95.5 0.5 0.0 0.0 4.0 0.0 61492 99.0 1.0 0.0 0.0 0.0 0.0 MEAN 98.5 0.6 0.1 0.0 0.8 0.0 SD 1.70 0.42 0.22 0.00 1.79 0.00 N 5 5 5 5 5 5 5 Appendix D (Table 2) WIL 417001 Acute Inhalation Toxicity (With Histopathology) Study of 1,1,2 Trichloroethane (1 ,l ,PTCE) in Rats Sponsor: HAP Task Force Individual Bronchoalveolar Lavage Fluid Leukocyte Differential Count (%)

FEMALES Group Animal No. No. Large Mononuclear Cells (%) Lymphocytes (%) Neutrophils (%) Eosinophils % Epithelial Cells (%) Mitotic Figures (%)

3 61448 96.0 2.0 0.0 0.0 2.0 0.0 61463 98.0 2.0 0.0 0.0 0.0 0.0 61468 98.0 1 .o 0.0 0.0 0.5 0.5 61483 97.0 1.5 0.0 0.0 1.5 0.0 61489 93.0 2.0 0.0 0.0 5.0 0.0 MEAN 96.4 1.7 0.0 0.0 1.8 0.1 SD 2.07 0.45 0.00 0.00 1.96 0.22 N 5 5 5 5 5 5 4 61445 97.5 1.5 1 .o 0.0 0.0 0.0 61460 96.0 3.0 0.5 0.0 0.0 0.5 61478 93.0 5.0 0.0 0.0 2.0 0.0 61485 100.0 0.0 0.0 0.0 0.0 0.0 MEAN 96.6 2.4 0.4 0.0 0.5 0.1 SD 2.93 2.14 0.48 0.00 1 .oo 0.25 N 4 4 4 4 4 4 6 Appendix D (Table 3) w IL 41 7001 Acute Inhalation Toxicity (With Histopathology) Study of 1 ,1,2 Trichloroethane (1 ,1,2 TCE) in Rats Sponsor: HAP Task Force Bronchoalveolar Lavage Fluid Leukocyte Counts  Summary of Means MALES Total Cell Large Epithelial Mitotic Group Count Mononuclear Lymphocytes Neutrophils Eosinophils Cells Figures No. (Xl O%/ ml) Cells (Xl O%/ ml) (Xl OS/ ml) (Xl O%/ ml) (Xl O%/ ml) (XlO%/ ml) (XlO% hl)

1 MEAN 1.7 1.7 0.012 0.000 0.000 0.006 0.000 SD 0.35 0.36 0.0082 0.0000 0.0000 0.0134 0.0000 N 5 5 5 5 5 5 5 2 MEAN 1.4 1.4 0.027 0.000 0.000 0.000 0.000 SD 0.50 0.54 0.0468 0.0000 0.0000 0.0000 0.0000 N 3 3 3 3 3 3 3 3 MEAN 1.5 1.5 0.013 0.000 0.000 0.006 0.002 SD 0.25 0.25 0.0127 0.0000 0.0000 0.0076 0.0040 N 4 4 4 4 4 4 4 4 MEAN 1.2 1.1 0.008 0.005 0.002 0.012 0.005 SD 0.36 0.35 0.0081 0.0066 0.0038 0.0177 0.0113 N 5 5 5 5 5 5 5 7 Appendix D (Table 3) WIL 417001 Acute Inhalation Toxicity (With Histopathology) Study of 1 ,1,2 Trichloroethane (1 ,1,2 TCE) in Rats Sponsor: HAP Task Force Bronchoalveolar Lavage Fluid Leukocyte Counts  Summary of Means FEMALES Total Cell Large Epithelial Mitotic Group Count Mononuclear Lymphocytes Neutrophils Eosinophils Cells Figures No. (Xl O/ ml) Cells (Xl O%/ ml) (Xl O%/ ml) (Xl O%/ ml) (Xl O%/ ml) (Xl O%/ ml) (Xl m/ ml)