(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:    10000 MG/M3

ANIMAL NUMBER:  IGL348F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  1

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  1     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         M              +                                            +

 2         A         M              +             +             +

 E

 3         A         F              +                                            +

 4         A         M              +             +             +

 5         A         M              +                                            +

 6         A         M              +             +             +

 7         A         M              +                                            +

 8         A         F              +             +             +

 9C        A         M              +                                            +

10         A         M              +            (A)            +

11         A         M              +                                           (b)

12         A         M              +             +             +

13         A         F              +                                            +

14         A         F              +             +             +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:  Fetus numbers 3 and 5 found with identification tags detached, numbers

      arbitrarily assigned for skeletal exams

(A) - HEAD:  Retinal fold; Right

(b) - SKELETAL/RIBS (L1):  Rudimentary; Bilateral

H-67

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:  10000 MG/M3

ANIMAL NUMBER:  IGL430F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  0     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         M              +                                            +

 2         A         M              +              +              +

 3         A         F              +                                            +

 4C        A         M              +              +              +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

H-68

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:  10000 MG/M3

ANIMAL NUMBER:  IGL409F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  0     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         F              +              +             +

 2         A         F              +                                            +

 3         A         F              +              +             +

 4         A         F              +                                            +

 5         A         F              +              +             +

 6         A         M              +                                            +

 7         A         F              +              +             +

 8C        A         F              +                                            +

 9         A         F              +              +             +

10         A         F              +                                            +

11         A         M              +              +             +

12         A         F              +                                            +

13         A         F              +              +             +

14         A         M              +                                            +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

H-69

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:    10000 MG/M3

ANIMAL NUMBER:  IGL421F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  0     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         M              +             +               +

 2         A         F              +                                            +

 3         A         M              +             +               +

 4C        A         F              +                                            +

 5         A         F              +             +               +

 6         A         F              +                                            +

 7         A         F              +             +               +

 E

 8         A         F              +                                            +

 9         A         M              +             +               +

10         A         F              +                                            +

11         A         M              +             +               +

12         A         F              +                                            +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

H-70

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:    10000 MG/M3

ANIMAL NUMBER:  IGL423F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  0     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         M              +              +              +

 2         A         M              +                                             +

 3         A         F              +              +              +

 4         A         F              +                                             +

 5         A         M              +              +              +

 6         A         M              +                                             +

 7         A         F              +              +              +

 8         A         F              +                                             +

 9         A         F              +              +              +

10         A         F              +                                             +

 EC

11         A         M              +              +              +

12         A         F              +                                             +

13         A         F              +              +              +

14         A         F              +                                             +

15         A         M              +              +              +

16         A         F              +                                             +

17         A         M              +              +              +

18         A         F              +                                             +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

H-71

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:    10000 MG/M3

ANIMAL NUMBER:  IGL440F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  1     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         M              +                                            +

 2         A         M              +              +              +

 3C        A         M              +                                            +

 4         A         M              +              +              +

 5         A         F              +                                           (a)

 6         A         M              +              +              +

 7         A         M              +                                            +

 8         A         F              +              +              +

 9         A         M              +                                            +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

(a) - SKELETAL/RIBS (L1): Rudimentary; Left

H-72

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:    10000 MG/M3

ANIMAL NUMBER:  IGL399F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  3     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         F              +              +              +

 2         A         M              +                                            +

 3         A         M              +              +              +

 4         A         F              +                                          (b,c)

 5         A         M              +              +              +

 6         A         F              +                                          (d,e)

 7C        A         F              +              +              +

 8         A         M              +                                            +

 9         A         M              +              +              +

10         A         M              +                                            +

11         A         F              +              +              +

12         A         F              +                                           (a)

13         A         M              +              +              +

14         A         M              +                                            +

15         A         M              +              +              +

16         A         F              +                                            +

17         A         M              +              +              +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

(a) - SKELETAL/STERNEBRAE (VI):  Misshapen

(b) - SKELETAL/VERTEBRAE (T12):  Bifid centra

(c) - SKELETAL/VERTEBRAE (T12 Anlage):  Dumbbell shaped centra

(d) - SKELETAL/VERTEBRAE (T11-12):  Bifid centra

(e) - SKELETAL/VERTEBRAE (T11-12 Anlage):  Dumbbell shaped centra

H-73

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:    10000 MG/M3

ANIMAL NUMBER:  IGL452F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  2     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         M              +                                             +

 2         A         M              +              +             +

 3         A         F              +                                             +

 4         A         M              +              +             +

 5         A         M              +                                            (a)

 6C        A         F              +              +             +

 7         A         M              +                                            (b)

 8         A         M              +              +             +

 9         A         M              +                                             +

 E

10         A         F              +              +             +

11         A         F              +                                             +

12         A         F              +              +             +

13         A         F              +                                             +

14         A         F              +              +             +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

(a) - SKELETAL/STERNEBRAE (VI):  Misshapen

(b) - SKELETAL/RIBS (L1): Rudimentary; Left

H-74

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:  10000 MG/M3

ANIMAL NUMBER:  IGL436F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  1     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         M              +                                            +

 2         A         M              +              +             +

 3         A         M              +                                            +

 4         A         M              +              +             +

 5         A         M              +                                            +

 6         A         F              +              +             +

 EC

 7         A         F              +                                            +

 8         A         M              +              +             +

 9         A         M              +                                         (a,b,c)

10         A         M              +              +             +

11         A         M              +                                            +

12         A         F              +              +             +

13         A         M              +                                            +

14         A         M              +              +             +

15         A         F              +                                            +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

(a) - SKELETAL/STERNEBRAE (V): Unossified

(b) - SKELETAL/VERTEBRAE (T8): Dumbell centra

(c) - SKELETAL/VERTEBRAE (T8 Anlage): Dumbbell shaped centra

H-75

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:  10000 MG/M3

ANIMAL NUMBER:  IGL454F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  4     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         M              +                                           (a)

 2         A         M              +             +               +

 3         A         M              +                                           (b)

 E

 4         A         F              +             +               +

 5         A         F              +                                            +

 6         A         F              +             +               +

 7         A         F              +                                           (a)

 8C        A         F              +             +               +

 9         A         F              +                                           (c)

 E

10         A         F              +             +               +

11         A         M              +                                            +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

(a) - SKELETAL/RIBS: (L1):  Rudimentary; Left

(b) - SKELETAL/RIBS: (L1):  Rudimentary; Bilateral

(c) - SKELETAL/VERTEBRAE (T12 Anlage):  Dumbbell shaped centra

H-76

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:    10000 MG/M3

ANIMAL NUMBER:  IGL461F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  1     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  2     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         F              +              +            +

 2         A         F              +                                             +

 3         A         M              +              +            +

 4         A         M              +                                             +

 5         A         M              +              +            +

 6C        A         F              +                                            (b)

 7         A         F              +              +            +

 8         A         M              +                                            (c)

 9         A         F              +              +           (a)

10         A         M              +                                             +

11         A         M              +              +            +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

(a) - ABDOMEN/THORAX:  Umbilical artery arises from left side of urinary bladder

(b) - SKELETAL/FOREPAW (Metacarpal 1 anlage):  Misshapen; Left

(c) - SKELETAL/RIBS (L1):  Rudimentary; Left

H-77

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:    10000 MG/M3

ANIMAL NUMBER:  IGL469F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  0     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 C1        A         F              +              +              +

 2         A         M              +                                            +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

H-78

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:    10000 MG/M3

ANIMAL NUMBER:  IGL470F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  4     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         F              +                                          (b,c)

 2         A         M              +             +             +

 3         A         F              +                                            +

 4         A         M              +             +             +

 5         A         M              +                                            +

 6         A         M              +             +             +

 7         A         M              +                                         (d,e,f)

 8         A         M              +             +             +

 9         A         M              +                                            +

10         A         M              +             +             +

11         A         F              +                                            +

12C        A         M              +             +             +

13         A         F              +                                           (g)

14         A         M              +             +             +

15         A         F              +                                            +

16         A         M              +             +             +

17         A         M              +                                          (a,h)

18         A         F              +             +             +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

(a) - SKELETAL/RIBS (L1):  Rudimentary; Left

(b) - SKELETAL/VERTEBRAE (T10):  Bifid centra

(c) - SKELETAL/VERTEBRAE (T10 Anlage):  Dumbbell shaped centra

(d) - SKELETAL/VERTEBRAE (T8):  Dumbbell shaped centra

(e) - SKELETAL/VERTEBRAE (T11):  Bifid centra

(f) - SKELETAL/VERTEBRAE (T11 Anlage):  Dumbbell shaped centra

(g) - SKELETAL/VERTEBRAE (T10-11 Anlage):  Dumbbell shaped centra

				(h) - SKELETAL/VERTEBRAE (L1):  Dumbbell shaped centra

H-79

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:  10000 MG/M3

ANIMAL NUMBER:  IGL473F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  2     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         F              +              +               +

 2         A         M              +                                            +

 3         A         M              +              +               +

 E

 4         A         F              +                                           (a)

 5         A         F              +              +               +

 6         A         F              +                                            +

 7C        A         M              +              +               +

 8         A         F              +                                            +

 9         A         F              +              +               +

10         A         F              +                                            +

11         A         M              +              +               +

12         A         M              +                                           (b)

13         A         M              +              +               +

14         A         F              +                                            +

15         A         M              +              +               +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

(a) - SKELETAL/RIBS (L1): Rudimentary; Left

(b) - SKELETAL/RIBS (L1): Rudimentary; Bilateral

H-80

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:    10000 MG/M3

ANIMAL NUMBER:  IGL407F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  2     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  3     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         F              +              +               +

 2         A         F              +                                            +

 3         A         F              +              +               +

 4         A         M              +                                           (c)

 5         A         M              +              +              (a)

 6         A         M              +                                            +

 7         A         M              +              +               +

 8         A         F              +                                            +

 9         A         F              +              +               +

10         A         F              +                                           (b)

11C        A         F              +              +              (a)

12         A         F              +                                            +

13         A         F              +              +               +

14         A         F              +                                            +

15         A         M              +              +               +

16         A         M              +                                           (c)

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

(a) - ABDOMEN/THORAX:  Umbilical artery arises from left side of urinary bladder

(b) - SKELETAL/STERNEBRAE (VI):  Hypoplastic

(c) - SKELETAL/RIBS (L1):  Rudimentary; Bilateral

H-81

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:    10000 MG/M3

ANIMAL NUMBER:  IGL442F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  1     MALFORMATIONS:  1

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         F              +                                             +

 2         A         F              +             +                +

 3         A         F              +                                             +

 4         A         F              +             +                +

 5C        A         M              +                                             +

 6         A         M              +             +                +

 7         A         F              +                                             +

 8         A         F              +             +                +

 9         A         F              +                                             +

10         A         M              +             +                +

11         A         F              +                                           (a,B)

12         A         M              +             +                +

13         A         F              +                                             +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

(a) - SKELETAL/RIBS (T13):  Short last rib; Right

(B) - SKELETAL/VERTEBRAE (L): One less presacral vertebrae

H-82

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:  20000 MG/M3

ANIMAL NUMBER:  IGL364F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  3     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         F              +                                             +

 2         A         M              +             +               +

 3         A         M              +                                             +

 4         A         F              +             +               +

 5         A         F              +                                             +

 6C        A         M              +             +               +

 7         A         M              +                                            (a)

 8         A         M              +             +               +

 9         A         M              +                                             +

10         A         M              +             +               +

11         A         F              +                                             +

12         A         M              +             +               +

13         A         F              +                                             +

14         A         M              +             +               +

15         A         F              +                                            (a)

16         A         M              +             +               +

17         A         F              +                                            (b)

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

(a) - SKELETAL/RIBS (L1):  Rudimentary; Left

(b) - SKELETAL/RIBS (L1):  Rudimentary; Bilateral

H-83

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:  20000 MG/M3

ANIMAL NUMBER:  IGL367F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  2     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         M              +             +              +

 2         A         M              +                                             +

 3         A         M              +             +              +

 4         A         M              +                                             +

 5         A         M              +             +              +

 6         A         M              +                                             +

 7         A         F              +             +              +

 8C        A         M              +                                            (a)

 9         A         F              +             +              +

10         A         M              +                                             +

11         A         F              +             +              +

12         A         M              +                                            (b)

13         A         F              +             +              +

14         A         M              +                                             +

15         A         M              +             +              +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

(a) - SKELETAL/STERNEBRAE (V):  Unossified

(b) - SKELETAL/RIBS (L1):  Rudimentary; Left

H-84

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:    20000 MG/M3

ANIMAL NUMBER:  IGL368F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  1     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         F              +                                            +

 2         A         M              +             +            +

 3         A         M              +                                            +

 4         A         F              +             +            +

 5         A         M              +                                            +

 6*        A         M              +             +            +

 7         A         M              +                                            +

 8C        A         F              +             +            +

 9         A         M              +                                            +

10         A         M              +             +            +

11         A         M              +                                            +

12         A         M              +             +            +

13         A         F              +                                           (a)

14         A         F              +             +            +

15         A         M              +                                            +

16         A         F              +             +            +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

 *  - Stunted

(a) - SKELETAL/VERTEBRAE (T9):  Dumbbell shaped centra

H-85

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:  20000 MG/M3

ANIMAL NUMBER:  IGL375F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  2     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 L

 1         A         F              +             +             +

 2         A         M              +                                            +

 3         A         M              +             +             +

 4         A         F              +                                           (c)

 5         A         F              +             +             +

 6         A         M              +                                            +

 7         A         M              +             +             +

 8C*       A         M              +                                          (a,b)

 9         A         M              +             +             +

10         A         M              +                                            +

11         A         M              +             +             +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

 *  - Stunted

(a) - SKELETAL/STERNEBRAE (V):  Unossified

(b) - SKELETAL/STERNEBRAE (VI): Misshapen

(c) - SKELETAL/RIBS (L1):  Rudimentary; Right

H-86

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:    20000 MG/M3

ANIMAL NUMBER:  IGL363F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  1     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         M              +              +              +

 2         A         M              +                                            +

 3         A         M              +              +              +

 4C        A         M              +                                           (a)

 5         A         M              +              +              +

 6         A         F              +                                            +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

(a) - SKELETAL/RIBS (L1): Rudimentary; Right

H-87

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:  20000 MG/M3

ANIMAL NUMBER:  IGL373F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  1     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         F              +             +              +

 2         A         F              +                                          (a,b)

 3         A         M              +             +              +

 E

 4         A         F              +                                            +

 5         A         F              +             +              +

 6         A         F              +                                            +

 7         A         F              +             +              +

 8C        A         M              +                                            +

 9         A         F              +             +              +

10         A         M              +                                            +

11         A         F              +             +              +

12         A         F              +                                            +

13         A         M              +             +              +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

(a) - SKELETAL/VERTEBRAE (T11):  Bifid centra

(b) - SKELETAL/VERTEBRAE (T6):  Dumbbell shaped centra

H-88

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:  20000 MG/M3

ANIMAL NUMBER:  IGL397F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  1     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         F              +              +              +

 2         A         M              +                                            +

 3         A         M              +              +              +

 4         A         M              +                                            +

 5         A         F              +              +              +

 6         A         F              +                                            +

 7         A         M              +              +              +

 8         A         M              +                                            +

 9         A         F              +              +              +

10         A         M              +                                            +

11C        A         F              +              +              +

12         A         M              +                                            +

13         A         F              +              +              +

14         A         F              +                                           (a)

15         A         M              +              +              +

16         A         F              +                                            +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

- SKELETAL/RIBS (L1): Rudimentary; Left

H-89

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:  20000 MG/M3

ANIMAL NUMBER:  IGL357F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  5     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1*        D         F              +              +             +

 2         A         F              +                                            +

 3*        A         M              +              +             +

 4*        A         F              +                                          (a,b)

 5         A         F              +              +             +

 6         A         F              +                                           (c)

 7         A         M              +              +             +

 8         A         F              +                                            +

 9         A         F              +              +             +

10         A         M              +                                          (c,d)

11         A         M              +              +             +

12C        A         M              +                                          (d,e)

13         A         F              +              +             +

14         A         M              +                                            +

15*        A         F              +              +             +

16*        A         F              +                                          (c,e)

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

 *  - Stunted

(a) - SKELETAL/STERNEBRAE (II-IV):  Dumbbell-shaped

(b) - SKELETAL/STERNEBRAE (V-VI):   Unossified

(c) - SKELETAL/STERNEBRAE (V):  Unossified

(d) - SKELETAL/STERNEBRAE (VI):  Asymmetric form

(e) - SKELETAL/STERNEBRAE (IV):  Dumbbell-shaped

H-90

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:  20000 MG/M3

ANIMAL NUMBER:  IGL369F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  3     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         F              +             +               +

 2         A         M              +                                           (a)

 3         A         F              +             +               +

 4         A         M              +                                            +

 E

 5C        A         F              +             +               +

 6         A         M              +                                            +

 7         A         M              +             +               +

 8         A         F              +                                           (c)

 9         A         F              +             +               +

10         A         F              +                                            +

11         A         F              +             +               +

12         A         F              +                                           (b)

13         A         M              +             +               +

14         A         F              +                                            +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

(a) - SKELETAL/STERNEBRAE (V):  Unossified

(b) - SKELETAL/STERNEBRAE (VI):  Misshapen

(c) - SKELETAL/RIBS (L1):  Rudimentary; Right

H-91

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:  20000 MG/M3

ANIMAL NUMBER:  IGL404F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  0     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1        A          M              +                                            +

 2        A          F              +             +             +

 3        A          F              +                                            +

 4        A          M              +             +             +

 5        A          M              +                                            +

 6C       A          F              +             +             +

 7        A          F              +                                            +

 8        A          F              +             +             +

 9        A          M              +                                            +

10        A          M              +             +             +

11        A          F              +                                            +

12        A          F              +             +             +

13        A          F              +                                            +

14        A          F              +             +             +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

H-92

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:    20000 MG/M3

ANIMAL NUMBER:  IGL365F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  0     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         M              +             +               +

 2         A         M              +                                            +

 3         A         M              +             +               +

 4         A         M              +                                            +

 5         A         F              +             +               +

 6         A         F              +                                            +

 E

 7C        A         M              +             +               +

 8         A         F              +                                            +

 9         A         M              +             +               +

10         A         F              +                                            +

11         A         M              +             +               +

12         A         M              +                                            +

13         A         F              +             +               +

14         A         M              +                                            +

15         A         F              +             +               +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

H-93

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:    20000 MG/M3

ANIMAL NUMBER:  IGL366F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  1

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  1     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1*        A         F              +                                          (b,c,d,e)

 2         A         M              +             +                 +

 3         A         F              +                                             +

 4         A         F              +            (A)                +

 5         A         M              +                                             +

 6         A         M              +             +                 +

 7C        A         M              +                                             +

 8         A         F              +             +                 +

 9         A         F              +                                             +

10         A         M              +             +                 +

11         A         M              +                                             +

12         A         F              +             +                 +

13         A         F              +                                             +

14         A         M              +             +                 +

15         A         M              +                                             +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

 *  - Stunted

(A) - HEAD:  Open eye; Right

(b) - SKELETAL/STERNEBRAE (II,III,VI):  Hypoplastic

(c) - SKELETAL/STERNEBRAE (V):  Unossified

(d) - SKELETAL/STERNEBRAE (IV):  Bifid

(e) - SKELETAL/RIBS (L1):  Rudimentary; Left

H-94

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:  20000 MG/M3

ANIMAL NUMBER:  IGL372F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  4     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         F              +                                            +

 2         A         F              +             +              +

 3         A         M              +                                           (a)

 4         A         M              +             +              +

 5         A         M              +                                           (b)

 6         A         M              +             +              +

 7         A         M              +                                            +

 8C        A         M              +             +              +

 9         A         F              +                                            +

10         A         F              +             +              +

11         A         M              +                                           (a)

 E

12         A         F              +             +              +

13         A         M              +                                           (a)

14         A         M              +             +              +

15         A         M              +                                            +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

(a) - SKELETAL/RIBS (L1):  Rudimentary; Right

(b) - SKELETAL/RIBS (L1):  Rudimentary; Left

H-95

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:    20000 MG/M3

ANIMAL NUMBER:  IGL396F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  0     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         M              +                                            +

 2         A         M              +              +              +

 3         A         F              +                                            +

 4         A         F              +              +              +

 5         A         F              +                                            +

 6C        A         M              +              +              +

 7         A         F              +                                            +

 8         A         F              +              +              +

 9         A         F              +                                            +

10         A         F              +              +              +

11         A         M              +                                            +

12         A         F              +              +              +

13         A         M              +                                            +

14         A         M              +              +              +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

H-96

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:    20000 MG/M3

ANIMAL NUMBER:  IGL371F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  2     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         F              +             +               +

 2         A         F              +                                             +

 3         A         M              +             +               +

 4         A         M              +                                            (a)

 5         A         F              +             +               +

 6         A         M              +                                             +

 7         A         F              +             +               +

 8         A         M              +                                            (b)

 EC

 9         A         F              +             +               +

10         A         M              +                                             +

11         A         F              +             +               +

12         A         F              +                                             +

13         A         M              +             +               +

14         A         F              +                                             +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

(a) - SKELETAL/RIBS (L1):  Rudimentary; Left

(b) - SKELETAL/RIBS (L1):  Rudimentary; Right

H-97

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:  20000 MG/M3

ANIMAL NUMBER:  IGL410F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  4     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         M              +                                           (b)

 2         A         M              +             +              +

 3         A         F              +                                           (c)

 4         A         F              +             +              +

 5         A         F              +                                          (a,d)

 6         A         F              +             +              +

 7         A         F              +                                            +

 8C        A         M              +             +              +

 9         A         F              +                                            +

10         A         M              +             +              +

11         A         M              +                                        (c,e,f,g)

12         A         F              +             +              +

13         A         F              +                                            +

14         A         F              +             +              +

15         A         M              +                                            +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

(a) - SKELETAL/STERNEBRAE (VI):  Misshapen

(b) - SKELETAL/RIBS (L1): Rudimentary; Bilateral

(c) - SKELETAL/RIBS (L1): Rudimentary; Right

(d) - SKELETAL/RIBS (L1): Rudimentary; Left

(e) - SKELETAL/RIBS (L1): Well-formed; Left

(f) - SKELETAL/VERTEBRAE (T12):  Bifid centra

(g) - SKELETAL/VERTEBRAE (T12 Anlage):  Dumbbell shaped centra

H-98

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:  20000 MG/M3

ANIMAL NUMBER:  IGL415F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  1

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  0     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         F              +             +              +

 2         A         M              +                                            +

 3         A         M              +             +              +

 4         A         F              +                                            +

 5         A         F              +             +              +

 6         A         F              +                                            +

 7         A         M              +             +              +

 8C        A         F              +                                            +

 9         A         M              +             +              +

10         A         M              +                                            +

11         A         M              +             +             (A)

12         A         F              +                                            +

13         A         M              +             +              +

14         A         M              +                                            +

15         A         M              +             +              +

16         A         F              +                                            +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

                                (A) - ABDOMEN/THORAX:  Umbilical artery aneurysm

H-99

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:    20000 MG/M3

ANIMAL NUMBER:  IGL444F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  3     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         F              +                                            +

 2         A         M              +              +              +

 3         A         F              +                                            +

 4         A         M              +              +              +

 5         A         M              +                                            +

 6         A         F              +              +              +

 7C        A         M              +                                         (a,c,d)

 8         A         F              +              +              +

 9         A         M              +                                            +

10         A         F              +              +              +

11         A         F              +                                           (b)

12         A         F              +              +              +

13         A         F              +                                           (b)

14         A         F              +              +              +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

(a) - SKELETAL/STERNEBRAE (V):  Misshapen

(b) - SKELETAL/RIBS (L1):  Rudimentary; Left

(c) - SKELETAL/VERTEBRAE (T11):  Bifid centra

(d) - SKELETAL/VERTEBRAE (T11 Anlage):  Dumbbell shaped centra

H-100

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:    20000 MG/M3

ANIMAL NUMBER:  IGL449F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  2     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         F              +             +             +

 2         A         M              +                                            +

 3         A         F              +             +             +

 4         A         F              +                                            +

 5         A         F              +             +             +

 6         A         F              +                                            +

 7         A         M              +             +             +

 8         A         M              +                                           (a)

 9         A         F              +             +             +

10C        A         F              +                                            +

11         A         F              +             +             +

12         A         M              +                                           (b)

13         A         M              +             +             +

14         A         M              +                                            +

15         A         M              +             +             +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

(a) - SKELETAL/RIBS (L1):  Rudimentary; Left

(b) - SKELETAL/VERTEBRAE (CE3 Anlage):  Bifid centra

H-101

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:  20000 MG/M3

ANIMAL NUMBER:  IGL458F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  0     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1        A          F              +                                            +

 2        A          M              +              +            +

 3        A          M              +                                            +

 4        A          F              +              +            +

 5C       A          F              +                                            +

 6        A          M              +              +            +

 7        A          M              +                                            +

 8        A          F              +              +            +

 9        A          F              +                                            +

10        A          M              +              +            +

11        A          M              +                                            +

12        A          F              +              +            +

13        A          F              +                                            +

14        A          F              +              +            +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

H-102

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:    20000 MG/M3

ANIMAL NUMBER:  IGL471F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  0     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         F              +              +              +

 2         A         F              +                                            +

 3         A         M              +              +              +

 4         A         F              +                                            +

 5         A         F              +              +              +

 6C        A         M              +                                            +

 7         A         F              +              +              +

 8         A         M              +                                            +

 9         A         F              +              +              +

10*        A         F              +                                            +

11         A         M              +              +              +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

 *  - Stunted

H-103

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:  20000 MG/M3

ANIMAL NUMBER:  IGL424F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  4     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         F              +                                           (a)

 2         A         F              +             +              +

 3         A         M              +                                           (b)

 E

 4         A         M              +             +              +

 5         A         M              +                                          (c,d)

 6         A         F              +             +              +

 7C        A         M              +                                            +

 8         A         M              +             +              +

 9         A         M              +                                            +

10         A         M              +             +              +

11         A         F              +                                            +

12         A         M              +             +              +

13         A         F              +                                            +

14         A         F              +             +              +

15         A         F              +                                           (a)

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

(a) - SKELETAL/STERNEBRAE (V):  Unossified

(b) - SKELETAL/STERNEBRAE (VI):  Misshapen

(c) - SKELETAL/VERTEBRAE (T10): Bifid centra

(d) - SKELETAL/VERTEBRAE (T10 Anlage): Dumbbell shaped centra

H-104

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:    20000 MG/M3

ANIMAL NUMBER:  IGL428F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  2     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         F              +                                           (a)

 2         A         M              +              +              +

 3         A         M              +                                            +

 4         A         F              +              +              +

 5         A         M              +                                            +

 6         A         M              +              +              +

 7         A         F              +                                           (a)

 8         A         F              +              +              +

 EC

 9         A         F              +                                            +

10         A         F              +              +              +

11         A         F              +                                            +

12         A         F              +              +              +

13         A         M              +                                            +

14         A         M              +              +              +

15         A         F              +                                            +

16         A         F              +              +              +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

(a) - SKELETAL/RIBS (L1): Rudimentary; Left

H-105

APPENDIX H - INDIVIDUAL FETAL OBSERVATIONS

(ABBREVIATIONS PRESENTED BELOW OBSERVATIONS)

TARGET DOSE:    20000 MG/M3

ANIMAL NUMBER:  IGL439F

NUMBER OF FETUSES WITH EXTERNAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH VISCERAL VARIATIONS:  0     MALFORMATIONS:  0

NUMBER OF FETUSES WITH SKELETAL VARIATIONS:  1     MALFORMATIONS:  0

FETUS | STATUS |    SEX    |     EXTERNAL     |          VISCERAL          |  SKELETAL  |

 NO.  |        |           |                  |  HEAD  |   ABDOMEN/THORAX  |            |

------|--------|-----------|------------------|--------|---------------- ---|------------|

 1         A         F              +              +             +

 2         A         F              +                                            +

 3         A         M              +              +             +

 4         A         F              +                                            +

 5         A         M              +              +             +

 6         A         F              +                                           (a)

 7         A         M              +              +             +

 8         A         M              +                                            +

 9C        A         F              +              +             +

10         A         M              +                                            +

11         A         F              +              +             +

12         A         M              +                                            +

13         A         M              +              +             +

14         A         M              +                                            +

15         A         M              +              +             +

16         A         F              +                                            +

A = ALIVE      M = MALE      E = EARLY RESORPTION      C = CERVIX

D = DEAD       F = FEMALE    L = LATE RESORPTION       + = NO OBSERVABLE ABNORMALITIES

NOTE:

(a) - SKELETAL/VERTEBRAE (T11): Bifid centra

H-106

APPENDIX I  -  INHALATION EXPOSURE DATA

TABLE OF CONTENTS

EXPOSURE CHAMBERS 		I-2

TEST ATMOSPHERE GENERATION		I-2

CHAMBER ENVIRONMENTAL CONDITIONS		I-2

ANALYTICAL PROCEDURES 		I-3

CHAMBER HOMOGENEITY 		I-4

LIGHTING, NOISE, AND OXYGEN LEVELS		I-4

PARTICLE SIZE ANALYSIS		I-4

LIST OF FIGURES

Figure		Page

FIGURE I-1.  Schematic of generation and exposure system		I-5

FIGURE I-2.  Schematic of analytical calibration system		I-6

FIGURE I-3.  Analytical calibration response curves		I-9

LIST OF TABLES

Table		Page

TABLE I-1.  Mean exposure data		I-7

TABLE I-2.  Gas chromatograph operating conditions		I-8

TABLE I-3.  Summary of exposure data		I-10

TABLE I-4.  Summary of chamber distribution sampling		I-19

TABLE I-5.  Lighting, noise, and oxygen levels		I-20

TABLE I-6.  Particle size data		I-21

TABLE I-7.  Chamber temperatures and humidities		I-23

I-1

APPENDIX I  -  INHALATION EXPOSURE DATA

Exposure Chambers

The chambers used in this study were constructed of stainless steel and glass and had a total volume of approximately 1.0 m3.  They were operated at an airflow rate of 200 liters per minute ensuring 12 air changes per hour and a theoretical equilibration time (T99) of 23 minutes.  The chamber volume and airflow were considered adequate to ensure an oxygen level greater than 19% and an animal loading below 5%.  The chamber airflow rate was monitored continuously with a calibrated flow-limiting orifice and differential pressure gauge and recorded approximately every 30 minutes.  All of the chambers were maintained at a slight negative pressure to the room.

Test Atmosphere Generation

Figure I-1 presents a schematic of the test atmosphere generation and exposure system.

Aliquots of the test substance for use in the daily conduct of the study were received in gas cylinders (~20 L nominal volume) via a specially constructed distribution manifold.  A manifold system maintained the test substance under nitrogen pressure,  ensuring that the transferred substance remained in liquid phase and retained the same composition as the original container.

The test substance was delivered via a diptube from the outlet valve of the cylinder to a variable area rotameter which regulated the rate of liquid flow into a heated glass round-bottom flask.  The test substance volatilized within the flask and the resulting vapors mixed with the supply air as they were drawn into the exposure chamber.

Chamber Environmental Conditions

Chamber temperature and humidity were monitored by wet/dry bulb hygrometers and recorded at approximately thirty minute intervals throughout each exposure.

I-2

APPENDIX I  -  INHALATION EXPOSURE DATA

Analytical Procedures

Schematic of the analytical calibration system: Figure I-2.

Analytical calibration response curve: Figure I-3

Mean exposure data: Table I-1

Gas chromatograph operating conditions: Table I-2

Summary of exposure data: Table I-3

Exposure concentrations were determined on both a nominal and analytical basis.  Nominal concentrations for each exposure level were calculated by weighing the tank containing the test substance before and after exposure and dividing the net loss in weight by the total volume of air passing through the chamber during the exposure.

Analytical exposure concentrations were determined hourly during each exposure by on-line gas chromatography (Hewlett Packard 6890).  Samples of the chamber atmosphere were continuously delivered to the GC via 1/8” teflon tubing connected to an automated 12-port multiposition gas sampling valve.  The multiposition valve was programmed to sequentially direct each stream to a sample loop which injected a fixed sample volume (2 cc) directly onto the column for analysis.  A complete sampling cycle was performed during each hour of exposure.

The analytical system was calibrated against a series of known concentrations of the test substance in air.  The air concentrations were determined by injecting a weighed amount of the test substance from a gas-tight syringe into a closed loop system of a known air volume (see schematical drawing).  The closed loop system consisted of an infrared vapor analyzer (MIRAN 1A-CVF, Foxboro Analytical) connected to the gas sampling valve of the GC.  A metal bellows pump was used to circulate the injected test substance vapors through the sample cell of the infrared monitor and the gas sampling valve of the GC.  The response of the infrared analyzer was monitored until the air concentration appeared equilibrated and stable,  then three replicate samples were taken using the gas sample loop of the GC.  The entire closed loop system was cleared with clean air between each calibration injection.  The average response of the GC (total peak area) for the four main constituents of the atmosphere, at each air concentration was used to construct a linear calibration for the test substance.

This method permitted the GC and the infrared analyzer (a backup analytical method) to be calibrated simultaneously and  under conditions similar to actual chamber sampling.  Once established, the calibration was checked daily during the study by sampling a certified standard of n-butane, the major component of the test substance mixture.

I-3

APPENDIX I  -  INHALATION EXPOSURE DATA

Chamber Homogeneity

Table I-4 presents a summary of the chamber distribution data.

During the method development trials for this study, samples were drawn from twelve different points within the chamber at each target concentration to demonstrate the homogeneity of test atmosphere distribution.

Lighting, Noise and Oxygen Levels

Table I-5 presents a summary of the lighting, noise, and oxygen level data.

Light intensity was measured three times during the study (the first day of exposures, during the second week of exposures, and the last day of exposures) in both the animal room and the chamber room.  Light intensity was measured in the animal room in a cage approximately three feet above the floor level.  In the chamber room the light intensity was measured three feet above floor level in the approximate center of each generation room.

Additionally, the oxygen level and the noise level were measured in each exposure chamber on the first day of exposures, during the second week of exposures, and on the last day of exposures.

Particle Size Analysis.

Table I-6 presents a summary of the particle size data.

A particle size determination of the aerosol portion of the test atmosphere was conducted during the chamber trials from the control and 20,000 mg/m3 target concentration chambers.  The particle size sampling indicated aerosol concentrations of 0 and 20 mg/m3 for the control and 20,000 mg.m3 target concentration chambers.  These small concentrations of aerosol were likely due to animal hair or dander.

The sample was taken using a multistage cascade impactor.  Preweighed glass fiber filters were used to collect aerosol on each stage.  Each stage was associated with specific cutoff diameters for aerodynamic particle size in microns.

The flow of air and the duration of the flow were recorded in the data.  This provided the information needed to convert the amount of aerosol captured in the cascade impactor to a chamber concentration.

I-4

APPENDIX I  -  INHALATION EXPOSURE DATA

FIGURE I-1  -  SCHEMATIC OF GENERATION AND EXPOSURE SYSTEM

I-5

 APPENDIX I  -  INHALATION EXPOSURE DATA

FIGURE I-2  -  SCHEMATIC OF ANALYTICAL CALIBRATION SYSTEM

I-6

APPENDIX I  -  INHALATION EXPOSURE DATA

TABLE I-1  - MEAN EXPOSURE DATA

GROUP:	1	2	3	4

Target Exposure Concentration

(mg/m3)

0

2000

10000

20000

Mean Analytical Exposure Concentration

(mg/m3)

0

1988

10327	20541

Average Chamber Temperature

(OF)

69

72

75

72

Average Chamber Relative Humidity

(% RH)

62

58

54

58

I-7

APPENDIX I  -  INHALATION EXPOSURE DATA

TABLE I-2  -  GAS CHROMATOGRAPH OPERATING CONDITIONS

GAS CHROMATOGRAPH:	Hewlett Packard 6890GC

DETECTOR:	Flame Ionization

COLUMN:	Supelco Part # 13867, MTO-SS, 2M 1/8”,

	Carbopack C 80/100 0.19% Picric Acid 15g

GAS FLOWS (cc/min):	H2  -  45.0

	Air  -  450.0

	Makeup Gas (Helium)  -  30.0

INLET TEMPERATURE:	100oC

INLET FLOW (cc/min)	Helium - 19.8

OVEN TEMPERATURE:	110oC

DETECTOR TEMPERATURE:	250oC

SAMPLE LOOP SIZE:	2 cc

ATTENUATION:	0

RUN TIME:	8.5 Minutes

I-8

APPENDIX I  -  INHALATION EXPOSURE DATA

FIGURE  I-3 - ANALYTICAL CALIBRATION RESONSE CURVES

I-9

APPENDIX I  -  INHALATION EXPOSURE DATA

TABLE I-3  -  SUMMARY OF EXPOSURE DATA

	Group 1	Group 2	Group 3	Group 4

Date	Mean	Nominal	Chamber	Mean	Nominal	Chamber	Mean	Nominal	Chamber	Mean Nominal	Chamber

	(mg/m3)	(mg/m3)	oF	% Rh	(mg/m3)	(mg/m3)	oF	% Rh	(mg/m3)	(mg/m3)	oF	% Rh (mg/m3)	(mg/m3)	oF	% Rh

27-Apr-02	0	0	67	64	1733	2088	69	59	10392	10360	71	58	21400	19922	69	59

28-Apr-02	0	0	68	64	1991	2089	70	58	10622	9388	74	51	21646	19743	70	57

29-Apr-02	0	0	68	55	2179	1933	70	50	10699	10363	74	47	21121	19524	70	57

30-Apr-02	0	0	68	65	2050	1950	70	58	10443	10272	73	58	21167	19432	70	59

1-May-02	0	0	70	59	1979	2375	71	58	10736	10581	75	49	20984	19547	72	55

2-May-02	0	0	69	64	2076	2069	71	64	10293	10335	75	54	20177	18992	72	61

3-May-02	0	0	70	58	1929	1924	72	61	10304	10297	76	50	21320	19390	72	58

4-May-02	0	0	68	64	2016	1736	71	61	9793	9913	74	59	20473	19208	72	56

5-May-02	0	0	68	64	1967	1924	74	52	10652	10435	75	53	20603	19501	72	58

6-May-02	0	0	69	70	1913	1896	71	69	9762	10168	75	61	20594	19035	72	65

7-May-02	0	0	70	65	1965	1981	73	62	10297	10306	76	57	20438	19501	73	61

8-May-02	0	0	70	62	1910	1914	72	59	9816	10056	75	58	19934	18799	73	61

9-May-02	0	0	70	58	1965	1856	73	59	10389	10632	75	57	20077	19921	71	62

10-May-02	0	0	70	65	2022	1976	73	60	10224	10246	76	55	19785	18965	73	62

11-May-02	0	0	71	65	1941	1953	75	59	10367	10443	76	55	20145	19547	74	59

12-May-02	0	0	70	69	2134	2032	74	59	10270	10435	75	61	20100	19274	74	66

13-May-02	0	0	70	72	1943	1956	74	59	10232	10364	76	59	20057	19578	74	61

14-May-02	0	0	70	55	2076	2018	72	50	10441	10611	76	47	20251	19531	73	49

15-May-02	0	0	69	61	2034	2032	72	57	10422	10646	75	54	20642	19526	73	54

16-May-02	0	0	68	58	1982	1969	70	58	10565	10789	75	52	20162	19350	71	54

17-May-02	0	0	68	64	2008	2093	70	58	10431	10606	74	54	20615	19790	71	58

18-May-02	0	0	68	59	1909	2244	71	51	9805	10442	74	50	20649	19551	71	54

19-May-02	0	0	68	65	1906	1936	72	58	10598	10763	73	59	20386	19767	71	58

20-May-02	0	0	66	63	2032	1988	70	58	10173	10385	73	53	21014	20071	70	58

21-May-02	0	0	67	54	2033	2035	69	56	10449	10628	74	47	19793	19257	70	54

MEAN	0	0	69	62	1988	1999	72	58	10327	10379	75	54	20541	19469	72	58

S.D.	0	0	1.3	4.6	87.7	124.2	1.6	4.2	280.2	293.4	1.2	4.4	518.3	314.5	1.4 3.8

Minimum	0	0	66	54	1733	1736	69	50	9762	9388	71	47	19785	18799	69	49

Maximum	0	0	71	72	2179	2375	75	69	10736	10789	76	61	21646	20071	74	66

I-10

APPENDIX I  -  INHALATION EXPOSURE DATA

TABLE I-3  -  SUMMARY OF EXPOSURE DATA (CONT’D)

GROUP 1  -  0 MG/M3 TARGET CONCENTRATION

Nominal	Mean Analytical 	Mean	Mean

	Exposure	Concentration	Concentration	Temperature	Relative Humidity

Date	Number	(mg/m3)	(mg/m3)	(oF)	(%)

27-Apr-02	1	0	0	67	64

28-Apr-02	2	0	0	68	64

29-Apr-02	3	0	0	68	55

30-Apr-02	4	0	0	68	65

1-May-02	5	0	0	70	59

2-May-02	6	0	0	69	64

3-May-02	7	0	0	70	58

4-May-02	8	0	0	68	64

5-May-02	9	0	0	68	64

6-May-02	10	0	0	69	70

7-May-02	11	0	0	70	65

8-May-02	12	0	0	70	62

9-May-02	13	0	0	70	58

10-May-02	14	0	0	70	65

11-May-02	15	0	0	71	65

I-11

APPENDIX I  -  INHALATION EXPOSURE DATA

TABLE I-3  -  SUMMARY OF EXPOSURE DATA (CONT’D)

GROUP 1  -  0 MG/M3 TARGET CONCENTRATION

Nominal	Mean Analytical 	Mean	Mean

	Exposure	Concentration	Concentration	Temperature	Relative Humidity

Date	Number	(mg/m3)	(mg/m3)	(oF)	(%)

12-May-02	16	0	0	70	69

13-May-02	17	0	0	70	72

14-May-02	18	0	0	70	55

15-May-02	19	0	0	69	61

16-May-02	20	0	0	68	58

17-May-02	21	0	0	68	64

18-May-02	22	0	0	68	59

19-May-02	23	0	0	68	65

20-May-02	24	0	0	66	63

21-May-02	25	0	0	67	54

Mean

0	0	69	62

S.D.

0	0	1.3	4.6

I-12

APPENDIX I  -  INHALATION EXPOSURE DATA

TABLE I-3  -  SUMMARY OF EXPOSURE DATA (CONT'D)

GROUP 2  -  2000 MG/M3 TARGET CONCENTRATION

Nominal

	Mean 	Mean	Mean

	Exposure	Conc.	Hourly Analytical Concentrations (mg/m3)	Concentration Temperature	Relative

Date	Number	(mg/m3)	1	2	3	4	5	6	(mg/m3)	(oF)	Humidity (%)

27-Apr-02	1	2088	2103	2128	1891	1498	1733	1042	1733a	69	59

28-Apr-02	2	2089	2159	1916	2035	2017	1785	2032	1991	70	58

29-Apr-02	3	1933	2135	2215	2274	2203	2149	2099	2179	70	58

30-Apr-02	4	1950	1923	2066	2074	2090	2178	1970	2050	70	58

1-May-02	5	2375	2020	2049	1874	2263	2051	1615	1979	71	58

2-May-02	6	2069	2101	2269	1978	2092	1898	2120	2076	71	64

3-May-02	7	1924	591	2222	2393	2076	2064	2225	1929	72	61

4-May-02	8	1736	1941	2028	1968	1979	1969	2212	2016	71	61

5-May-02	9	1924	2052	1795	2064	1987	1913	1989	1967	74	52

6-May-02	10	1896	1805	2052	1937	1902	1819	1965	1913	71	69

7-May-02	11	1981	2002*	1972	2029	1964	1879	1946	1965	73	62

8-May-02	12	1914	2022	2058	1953	1867	1746	1812	1910	72	59

9-May-02	13	1856	1875	2117	2034	1943	1918	1905	1965	73	59

10-May-02	14	1976	1992	1963	1927	2113	2051	2085	2022	73	60

11-May-02	15	1953	1915	1777	1986	2037	1914	2019	1941	75	59

a – Low value due to problems with the rotameter

* - Backup analytical system used

I-13

APPENDIX I  -  INHALATION EXPOSURE DATA

TABLE I-3  -  SUMMARY OF EXPOSURE DATA (CONT'D)

GROUP 2  -  2000 MG/M3 TARGET CONCENTRATION

Nominal

	Mean 	Mean	Mean

	Exposure	Conc.	Hourly Analytical Concentrations (mg/m3)	Concentration Temperature	Relative

Date	Number	(mg/m3)	1	2	3	4	5	6	(mg/m3)	(oF)	Humidity (%)

12-May-02	16	2032	2099	2099	2238	2110	2201	2058	2134	74	59

13-May-02	17	1956	1797	2044	2035	2112	2004	1663	1943	74	59

14-May-02	18	2018	2294	1988	2065	2048	1825	2238	2076	72	50

15-May-02	19	2032	1964	2078	2081	2142	2082	1859	2034	72	57

16-May-02	20	1969	2204	2131	2050	1623	1797	2089	1982	70	58

17-May-02	21	2093	2016	1977	2051	2041	2104	1858	2008	70	58

18-May-02	22	2244	1492	2346	1787	1858	2003	1966	1909	71	51

19-May-02	23	1936	1893	1861	1759	1784	2054	2085	1906	72	58

20-May-02	24	1988	2041	2177	2045	2073	1946	1912	2032	70	58

21-May-02	25	2035	1939	2112	2119	2020	2094	1913	2033	69	56

MEAN

1999

	1988	72	58

S.D.

124.2

	87.7	1.7	3.8

* - Backup analytical system used

I-14

APPENDIX I  -  INHALATION EXPOSURE DATA

TABLE I-3  -  SUMMARY OF EXPOSURE DATA (CONT’D)

GROUP 3 -  10,000 MG/M3 TARGET CONCENTRATION

Nominal

	Mean	Mean	Mean

Exposure	Conc.	Hourly Analytical Concentration (mg/m3)	Concentration Temperature	Relative

Date	Number	(mg/m3)	1	2	3	4	5	6	(mg/m3)	(oF)	Humidity (%)

27-Apr-02	1	10360	10533	10729	8758	10228	11168	10937	10392	71	58

28-Apr-02	2	9388	10829	9642	9711	11446	10142	11960	10622	74	51

29-Apr-02	3	10363	10510	11120	11077	10638	10446	10405	10699	74	47

30-Apr-02	4	10272	9950	9289	11872	10315	10541	10691	10443	73	58

1-May-02	5	10581	9829	10506	11152	10795	11065	11068	10736	75	49

2-May-02	6	10335	10172	10378	10258	10357	10393	10202	10293	75	54

3-May-02	7	10297	10000	10292	10727	10374	10522	9906	10304	76	50

4-May-02	8	9913	9753	10292	11027	7441	9886	10357	9793	74	59

5-May-02	9	10435	10748	10508	10788	10802	10290	10777	10652	75	53

6-May-02	10	10168	9746	9073	10210	9397	10114	10029	9762	75	61

7-May-02	11	10306	11503*	10241	10112	10047	10012	9867	10297	76	57

8-May-02	12	10056	9346	9589	10209	9670	10041	10042	9816	75	58

9-May-02	13	10632	10227	10262	10671	10553	10346	10276	10389	75	57

10-May-02	14	10246	10185	9932	10648	10116	10135	10329	10224	76	55

11-May-02	15	10443	10078	10575	10666	10333	10090	10463	10367	76	55

* - Backup analytical system used

I-15

APPENDIX I  -  INHALATION EXPOSURE DATA

TABLE I-3  -  SUMMARY OF EXPOSURE DATA (CONT'D)

GROUP 3  -  10,000 MG/M3 TARGET CONCENTRATION

Nominal

	Mean 	Mean	Mean

	Exposure	Conc.	Hourly Analytical Concentration (mg/m3)	Concentration Temperature	Relative

Date	Number	(mg/m3)	1	2	3	4	5	6	(mg/m3)	(oF)	Humidity (%)

12-May-02	16	10435	9945	9988	10161	10471	10579	10474	10270	75	61

13-May-02	17	10364	9900	10068	10249	10655	10209	10309	10232	76	59

14-May-02	18	10611	10147	10293	10392	10403	10649	10763	10441	76	47

15-May-02	19	10646	9513	10424	10249	10989	10762	10595	10422	75	54

16-May-02	20	10789	10412	10416	10719	10693	10660	10493	10565	75	52

17-May-02	21	10606	10172	10584	10214	10408	10617	10591	10431	74	54

18-May-02	22	10442	9100	9745	9832	9864	10150	10137	9805	74	50

19-May-02	23	10763	9761	10432	11103	10674	10626	10993	10598	73	59

20-May-02	24	10385	9866	10368	10566	10192	10133	9916	10173	73	53

21-May-02	25	10628	10033	10466	10250	10716	10584	10647	10449	74	47

MEAN

10379

	10327	75	54

S.D.

293.4

	280.2	1.2	4.4

* - Backup analytical system used

I-16

APPENDIX I  -  INHALATION EXPOSURE DATA

TABLE I-3  -  SUMMARY OF EXPOSURE DATA (CONT'D)

GROUP 4 -  20,000 MG/M3 TARGET CONCENTRATION

Nominal

	Mean	Mean	Mean

Exposure	Conc.	Hourly Analytical Concentration (mg/m3)	Concentration Temperature	Relative

Date	Number	(mg/m3)	1	2	3	4	5	6	(mg/m3)	(oF)	Humidity (%)

27-Apr-02	1	19922	21229	21107	20717	21913	22589	20845	21400	69	59

28-Apr-02	2	19743	20894	21824	21540	22071	21998	21550	21646	70	57

29-Apr-02	3	19524	21334	21752	21230	20981	20733	20689	21121	70	57

30-Apr-02	4	19432	19740	20830	21230	21195	22460	21550	21167	70	59

1-May-02	5	19547	20214	20531	21389	21281	21086	21404	20984	72	55

2-May-02	6	18992	18611	19921	19585	21056	21148	20743	20177	72	61

3-May-02	7	19390	20737	21288	21456	21500	21580	21358	21320	72	57

4-May-02	8	19208	20495	21303	20488	19770	20119	20664	20473	72	56

5-May-02	9	19501	20313	21205	20829	20625	20536	20107	20603	72	58

6-May-02	10	19035	19444	20189	20716	17551	20521	25144	20594	72	65

7-May-02	11	19501	19382*	20626	20747	21406	19892	20576	20438	73	61

8-May-02	12	18799	18743	19573	20462	20183	20005	20638	19934	73	61

9-May-02	13	19921	20342	19990	20308	19972	18632	21216	20077	71	62

10-May-02	14	18965	19491	20230	20272	18387	20199	20133	19785	73	62

11-May-02	15	19547	15234	21088	20859	21692	20900	21095	20145	74	59

* - Backup analytical system used

I-17

APPENDIX I  -  INHALATION EXPOSURE DATA

TABLE I-3  -  SUMMARY OF EXPOSURE DATA (CONT'D)

GROUP 4  -  20,000 MG/M3  TARGET CONCENTRATION

Nominal

	Mean 	Mean	Mean

	Exposure	Conc.	Hourly Analytical Concentration (mg/m3)	Concentration Temperature	Relative

Date	Number	(mg/m3)	1	2	3	4	5	6	(mg/m3)	(oF)	Humidity (%)

12-May-02	16	19274	18154	19894	20787	20241	22009	19513	20100	74	66

13-May-02	17	19578	20298	19879	20010	19612	20170	20370	20057	74	61

14-May-02	18	19531	19506	20204	20905	19819	20526	20542	20251	73	49

15-May-02	19	19526	20123	20852	20415	20941	20783	20737	20642	73	54

16-May-02	20	19350	18785	19978	20442	20500	20312	20954	20162	71	54

17-May-02	21	19790	19989	20182	20241	20200	22775	20303	20615	71	58

18-May-02	22	19551	20381	20949	20809	20550	20767	20439	20649	71	54

19-May-02	23	19767	20283	18377	21781	21244	18671	21963	20386	71	58

20-May-02	24	20071	19828	20809	21286	20966	21738	21454	21014	70	58

21-May-02	25	19257	19289	20130	21021	16493	20883	20942	19793	70	54

MEAN

19469

	20541	72	58

S.D.

314.5

	518.3	1.4	3.8

I-18

APPENDIX I  -  INHALATION EXPOSURE DATA

TABLE  I-4 - SUMMARY OF DISTRIBUTION SAMPLES

SAMPLE

LOCATION	TARGET EXPOSURE LEVELS

	2000 MG/M3	10,000 MG/M3	20,000 MG/M3

Left Top Back	2073	10462	21155

Left Top Front	1965	10636	21532

Left Middle Back	2270	10385	20995

Left Middle Front	2183	10507	21408

Left Bottom Back	2184	10659	21124

Left Bottom Front	1926	10373	21294

Right Top Back	2135	10676	20832

Right Top Front	2031	10526	21550

Right Middle Back	1926	10997	21517

Right Middle Front	1886	10903	21399

Right Bottom Back	2162	10802	21026

Right Bottom Front	1960	10401	20969

MEAN	2058	10611	21233

S.D.	126.79	206.70	248.71

%CV	6.16	1.95	1.17

Minimum	1886	10373	20832

Maximum	2270	10997	21550

		NOTE:  Top, middle, bottom sample locations represent horizontal planes within exposure chamber.

		Left, right, front, back represent corners of each horizontal plane.  Samples taken in series approximately

	8 minute intervals.

I-19

APPENDIX I  -  INHALATION EXPOSURE DATA

TABLE I-5 LIGHTING, NOISE, AND OXYGEN LEVELS

Environmental Conditions

	April 27, 2002	May 8, 2002	May 21, 2002

Light Intensity: (fc)

	Room PE103  in a cage 3 feet above the floor.	8.9	3.8	5.3

Center of room PE 102 3 feet above the floor.	32.5	36.1	62

Center of room PE 110 3 feet above the floor.	34.1	28.9	45.3

Noise level:  (db)

	1m - 1: Door open	78.9	75.5	73.2

1m - 1: Through port	79.7	76.9	77.1

1m - 2: Door open	79.3	73.6	73.1

1m - 2: Through port	80.2	77.1	76.5

1m - 3: Door open	79.1	74.0	74.9

1m - 3: Through port	80.1	76.8	77.2

1m - 4: Door open	78.8	72.5	72.2

1m - 4: Through port	79.9	75.2	75.1

O2 Level: (%)

	(Reading upon removal)	No Alarm	No Alarm	No Alarms

1m - 1	20.7	20.3	19.9

1m - 2	20.7	20.4	20.2

1m - 3	20.7	20.3	19.7

1m - 4	20.7	20.2	19.9

1m-1, 1m-2, 1m-3, 1m-4 are exposure chamber designations.

fc = foot candles (measured with an Omega HHLM-2 Light Meter)

db = decibels (measured using an Omega HHSL-1 Sound Meter)

% = % oxygen (measured using a Biosystems Oxy Plus Single Sensor Gas Detector with an alarm at 19.5% O2)

I-20

APPENDIX I  -  INHALATION EXPOSURE DATA

TABLE  I-6 - PARTICLE SIZE DATA

0 MG/M3

 PRIVATE  IMPACTOR

STAGE	STAGE CONSTANT

((m)	FILTER WEIGHT

DIFFERENCE ((g)	PERCENT IN

SIZE RANGE

FILTER	0.30	0	0

8	0.54	0	0

7	0.84	0	0

6	1.50	0	0

5	2.60	0	0

4	4.10	0	0

3	6.80	0	0

2	17.0	0	0

1	28.0	0	0

TOTAL =0

	PARTICLE CONCENTRATION = 0 MG/M3

	PARTICLE SIZE DETERMINED WITH A SIERRA SERIES 210 CASCADE IMPACTOR

	CONDITIONS:

		SAMPLE FLOWRATE (Liters/Minute):  3		SAMPLE DURATION (Minutes):  3

		CALCULATION OF PARTICLE CONCENTRATION:

		SAMPLE VOLUME (Liters)= SAMPLE FLOW RATE*SAMPLE DURATION

		PARTICLE CONCENTRATION =

		((TOTAL FILTER WEIGHT DIFFERENCE /1000 (g/mg)/(SAMPLE VOLUME))*1000 L/M3

I-21

APPENDIX I  -  INHALATION EXPOSURE DATA (CONT'D)

TABLE  I-6 - PARTICLE SIZE DATA (CONT'D)

20,000 MG/M3 TARGET CONCENTRATION

 PRIVATE  IMPACTOR

STAGE	STAGE CONSTANT

((m)	FILTER WEIGHT

DIFFERENCE ((G)	PERCENT IN

SIZE RANGE

FILTER	0.30	0	0

8	0.54	10	5.6

7	0.84	70	38.9

6	1.50	0	0

5	2.60	50	27.8

4	4.10	40	22.2

3	6.80	0	0

2	17.0	0	0

1	28.0	10	5.6

TOTAL =180

	CONCENTRATION OF PARTICLES = 20 MG/M3

MEAN MEDIAN AERODYNAMIC DIAMETER = 2.368 (m

	PARTICLE SIZE DETERMINED WITH A SIERRA SERIES 210 CASCADE IMPACTOR

	CONDITIONS:

		SAMPLE FLOWRATE (Liters/Minute):  3		SAMPLE DURATION (Minutes):  3

		CALCULATION OF PARTICLE CONCENTRATION:

		SAMPLE VOLUME (Liters) = SAMPLE FLOW RATE*SAMPLE DURATION

		PARTICLE CONCENTRATION =

		((TOTAL FILTER WEIGHT DIFFERENCE /1000 (g/mg)/(SAMPLE VOLUME))*1000 L/M3

I-22

TABLE  I-7 – CHAMBER TEMPERATURES AND HUMIDITIES

CHAMBER TEMPERATURES (ºF) - 0 mg/m3 Target Concentration

	Time from Start of Exposure (Hours)

	0	0.5	1	1.5	2	2.5	3	3.5	4	4.5	5	5.5	6

27-Apr-02	64	66	66	66	68	68	68	68	68	68	68	68	68

28-Apr-02	66	68	68	68	68	68	68	68	68	68	68	68	68

29-Apr-02	68	68	68	68	68	68	68	68	68	68	68	68	68

30-Apr-02	66	66	68	68	68	68	68	68	68	68	68	68	68

1-May-02	68	68	68	70	70	70	70	70	70	70	70	70	70

2-May-02	68	68	68	68	68	68	68	68	70	70	70	70	70

3-May-02	68	70	70	70	70	70	70	70	70	70	70	70	70

4-May-02	68	68	68	68	68	68	68	68	68	68	68	68	68

5-May-02	68	68	68	68	68	68	68	68	68	68	68	68	68

6-May-02	64	64	68	68	70	70	70	70	70	70	70	70	70

7-May-02	68	70	70	70	70	70	70	70	70	70	70	70	70

8-May-02	66	68	70	70	70	70	70	70	70	70	70	70	70

9-May-02	68	70	70	70	70	70	70	70	70	70	70	70	70

10-May-02	67	69	69	70	70	70	70	70	70	70	70	70	70

11-May-02	68	70	70	72	72	72	72	72	72	72	72	72	72

12-May-02	68	68	70	70	70	70	70	70	70	70	70	70	70

13-May-02	68	70	70	70	70	70	70	70	70	70	70	70	70

14-May-02	68	70	70	70	70	70	70	70	70	70	70	70	70

15-May-02	66	68	68	68	70	70	70	70	70	68	68	68	68

16-May-02	68	68	68	68	68	68	68	68	68	68	68	68	68

17-May-02	68	68	68	68	68	68	68	68	68	68	68	68	68

18-May-02	66	66	66	68	68	68	68	68	68	68	68	68	68

19-May-02	66	68	68	68	68	68	68	68	68	68	68	68	68

20-May-02	66	66	66	66	66	66	66	66	66	66	66	66	66

21-May-02	66	66	66	66	66	66	66	66	68	68	68	68	68

I-23

TABLE  I-7 – CHAMBER TEMPERATURES AND HUMIDITIES

CHAMBER TEMPERATURES (ºF) - 2000 mg/m3 Target Concentration

	Time from Start of Exposure (Hours)

	0	0.5	1	1.5	2	2.5	3	3.5	4	4.5	5	5.5	6

27-Apr-02	66	68	68	68	70	70	70	70	70	70	70	70	70

28-Apr-02	68	70	70	70	70	70	70	70	70	70	70	70	70

29-Apr-02	68	70	72	72	72	70	70	70	70	70	70	70	70

30-Apr-02	68	70	70	70	70	70	70	70	70	70	70	70	70

1-May-02	70	70	70	70	70	70	70	70	70	72	72	72	72

2-May-02	70	70	70	70	70	70	70	70	72	72	72	72	72

3-May-02	68	70	70	70	70	70	74	74	74	74	74	74	74

4-May-02	70	70	71	71	71	71	71	71	71	71	71	71	71

5-May-02	70	72	74	74	74	74	74	74	74	74	74	74	74

6-May-02	66	70	70	70	70	70	72	72	72	74	74	74	74

7-May-02	70	70	72	72	72	72	74	74	74	74	74	74	74

8-May-02	68	70	71	71	73	73	73	73	73	73	73	73	73

9-May-02	68	72	72	72	72	72	72	74	74	74	74	74	74

10-May-02	68	70	70	70	74	74	74	74	74	74	74	74	74

11-May-02	70	72	74	74	74	76	76	76	76	76	76	76	76

12-May-02	70	74	74	74	74	74	74	74	74	74	74	74	74

13-May-02	70	70	72	74	74	74	76	76	76	76	74	76	76

14-May-02	68	70	70	72	72	72	74	74	74	74	74	72	72

15-May-02	68	70	72	72	74	74	72	74	74	70	72	72	72

16-May-02	68	70	70	70	70	70	70	70	70	70	70	70	70

17-May-02	68	70	70	70	70	70	70	70	70	72	72	72	72

18-May-02	66	70	70	71	72	72	72	72	72	72	72	72	72

19-May-02	68	72	72	72	72	72	72	72	72	72	72	72	72

20-May-02	66	68	70	70	70	70	70	70	70	70	70	70	70

21-May-02	66	68	68	70	70	70	70	70	70	70	70	70	70

I-24

TABLE  I-7 – CHAMBER TEMPERATURES AND HUMIDITIES

CHAMBER TEMPERATURES (ºF) – 10,000 mg/m3 Target Concentration

	Time from Start of Exposure (Hours)

	0	0.5	1	1.5	2	2.5	3	3.5	4	4.5	5	5.5	6

27-Apr-02	66	70	70	70	70	72	72	72	72	72	72	72	72

28-Apr-02	70	72	74	74	74	74	74	74	74	74	74	74	74

29-Apr-02	68	72	74	74	74	75	75	75	75	75	75	75	75

30-Apr-02	68	70	72	72	74	74	74	74	74	74	74	74	74

1-May-02	70	73	74	74	76	76	76	76	78	76	76	76	76

2-May-02	70	72	74	74	74	74	74	74	78	78	78	78	78

3-May-02	68	72	74	76	76	76	78	78	78	78	78	78	78

4-May-02	72	72	74	74	74	74	74	74	74	74	74	74	74

5-May-02	70	72	74	76	76	76	76	76	76	76	76	76	76

6-May-02	68	70	74	74	76	76	76	76	76	78	78	78	78

7-May-02	68	72	76	76	76	76	76	78	78	78	78	78	78

8-May-02	68	72	74	76	76	76	76	76	76	76	76	76	76

9-May-02	68	72	74	74	74	74	74	78	78	78	78	78	78

10-May-02	68	72	74	76	76	76	78	78	78	78	78	78	78

11-May-02	68	72	76	76	78	78	78	78	78	78	78	78	78

12-May-02	70	74	76	76	76	76	76	76	76	76	76	76	76

13-May-02	68	72	76	76	78	78	78	78	78	78	78	78	78

14-May-02	68	72	74	76	76	76	78	78	78	78	78	78	78

15-May-02	66	72	74	76	76	76	76	78	78	76	76	76	76

16-May-02	68	72	74	74	74	76	76	76	76	76	76	76	76

17-May-02	68	70	74	74	74	74	76	76	76	76	76	76	76

18-May-02	66	70	72	74	76	76	76	76	76	76	76	76	76

19-May-02	66	70	74	74	74	74	74	74	74	74	74	74	74

20-May-02	66	70	72	72	74	74	74	74	74	74	74	74	74

21-May-02	66	70	72	74	74	74	74	74	76	76	76	76	76

I-25

TABLE  I-7 – CHAMBER TEMPERATURES AND HUMIDITIES

CHAMBER TEMPERATURES (ºF) – 20,000 mg/m3 Target Concentration

	Time from Start of Exposure (Hours)

	0	0.5	1	1.5	2	2.5	3	3.5	4	4.5	5	5.5	6

27-Apr-02	66	68	68	68	70	70	70	70	70	70	70	70	70

28-Apr-02	70	70	70	70	70	70	70	70	70	70	70	70	70

29-Apr-02	68	70	70	70	70	70	70	70	70	70	70	70	70

30-Apr-02	66	68	70	70	70	70	70	70	70	70	70	70	70

1-May-02	70	70	70	72	72	72	72	72	74	74	74	74	74

2-May-02	68	70	70	70	70	72	72	72	74	74	74	74	74

3-May-02	68	70	70	72	72	72	74	74	74	74	74	74	74

4-May-02	66	70	72	72	72	72	72	74	74	74	74	74	74

5-May-02	70	70	72	72	72	72	72	72	72	72	72	72	72

6-May-02	66	66	70	72	74	74	74	74	74	74	74	74	74

7-May-02	68	70	72	72	72	74	74	74	74	74	74	74	74

8-May-02	68	70	72	72	74	74	74	74	74	74	74	74	74

9-May-02	66	70	72	72	72	72	72	72	72	72	72	72	72

10-May-02	66	69	70	72	74	74	74	74	74	74	74	74	74

11-May-02	66	70	72	74	74	76	76	76	76	76	76	76	76

12-May-02	69	73	74	74	74	74	74	74	74	74	74	74	74

13-May-02	68	70	72	74	74	74	74	74	74	74	76	76	76

14-May-02	68	70	72	72	72	74	74	74	74	74	74	74	74

15-May-02	66	70	72	72	74	74	74	74	74	74	74	74	74

16-May-02	68	70	70	70	70	72	72	72	72	72	72	72	72

17-May-02	68	70	70	70	72	72	72	72	72	72	72	72	72

18-May-02	66	68	70	70	70	72	72	72	72	72	72	72	72

19-May-02	66	68	72	72	72	72	72	72	72	72	72	72	72

20-May-02	66	68	70	70	70	70	70	70	70	70	70	70	70

21-May-02	66	68	68	70	70	70	70	70	72	72	72	72	72

I-26

TABLE  I-7 – CHAMBER TEMPERATURES AND HUMIDITIES (CONT’D)

CHAMBER HUMIDITIES (%RH) - 0 mg/m3 Target Concentration

	Time from Start of Exposure (Hours)

	0	0.5	1	1.5	2	2.5	3	3.5	4	4.5	5	5.5	6

27-Apr-02	70	62	62	62	64	64	64	64	64	64	64	64	64

28-Apr-02	62	64	64	64	64	64	64	64	64	64	64	64	64

29-Apr-02	55	55	55	55	55	55	55	55	55	55	55	55	55

30-Apr-02	71	71	64	64	64	64	64	64	64	64	64	64	64

1-May-02	64	64	64	57	57	57	57	57	57	57	57	57	57

2-May-02	64	64	64	64	64	64	64	64	64	64	64	64	64

3-May-02	64	57	57	57	57	57	57	57	57	57	57	57	57

4-May-02	64	64	64	64	64	64	64	64	64	64	64	64	64

5-May-02	64	64	64	64	64	64	64	64	64	64	64	64	64

6-May-02	100	90	76	72	64	64	64	64	64	64	64	64	64

7-May-02	72	64	64	64	64	64	64	64	64	64	64	64	64

8-May-02	71	72	60	60	60	60	60	60	60	60	60	60	60

9-May-02	64	57	57	57	57	57	57	57	57	57	57	57	57

10-May-02	71	64	64	64	64	64	64	64	64	64	64	64	64

11-May-02	72	64	64	57	65	65	65	65	65	65	65	65	65

12-May-02	72	72	68	68	68	68	68	68	68	68	68	68	68

13-May-02	72	72	72	72	72	72	72	72	72	72	72	72	72

14-May-02	55	57	48	48	48	48	48	57	64	64	64	57	57

15-May-02	62	64	64	64	57	57	57	57	57	64	64	64	64

16-May-02	64	64	64	64	55	55	55	55	55	55	55	55	55

17-May-02	64	64	64	64	64	64	64	64	64	64	64	64	64

18-May-02	62	62	62	55	55	55	55	55	55	55	64	64	64

19-May-02	71	64	64	64	64	64	64	64	64	64	64	64	64

20-May-02	62	62	62	62	71	71	62	62	62	62	62	62	62

21-May-02	53	53	53	53	53	53	53	53	55	55	55	55	55

I-27

TABLE  I-7 – CHAMBER TEMPERATURES AND HUMIDITIES (CONT’D)

CHAMBER HUMIDITIES (%RH) - 2000 mg/m3 Target Concentration

	Time from Start of Exposure (Hours)

	0	0.5	1	1.5	2	2.5	3	3.5	4	4.5	5	5.5	6

27-Apr-02	62	64	64	64	57	57	57	57	57	57	57	57	57

28-Apr-02	64	57	57	57	57	57	57	57	57	57	57	57	57

29-Apr-02	64	57	50	50	50	48	48	48	48	48	48	48	48

30-Apr-02	64	57	57	57	57	57	57	57	57	57	57	57	57

1-May-02	64	57	57	57	57	57	57	57	57	57	57	57	57

2-May-02	64	64	64	64	64	64	64	64	65	65	65	65	65

3-May-02	64	64	64	64	64	64	58	58	58	58	58	58	58

4-May-02	64	64	60	60	60	60	60	60	60	60	60	60	60

5-May-02	64	57	51	51	51	51	51	51	51	51	51	51	51

6-May-02	90	76	76	76	76	76	65	65	65	58	58	58	58

7-May-02	64	72	65	65	65	65	58	58	58	58	58	58	58

8-May-02	72	57	57	57	58	58	58	58	58	58	58	58	58

9-May-02	72	57	57	57	57	57	57	58	58	58	58	58	58

10-May-02	72	64	64	64	58	58	58	58	58	58	58	58	58

11-May-02	64	65	58	58	58	52	59	59	59	59	59	59	59

12-May-02	68	58	58	58	58	58	58	58	58	58	58	58	58

13-May-02	72	72	65	58	58	58	52	52	52	52	66	52	52

14-May-02	55	48	57	50	50	50	44	44	44	51	51	50	50

15-May-02	55	57	57	57	51	51	57	58	58	64	57	57	57

16-May-02	64	57	57	57	57	57	57	57	57	57	57	57	57

17-May-02	64	57	57	57	57	57	57	57	64	57	57	57	57

18-May-02	62	52	52	53	50	50	50	50	50	50	50	50	50

19-May-02	64	57	57	57	57	57	57	57	57	57	57	57	57

20-May-02	62	64	57	57	57	57	57	57	57	57	57	57	57

21-May-02	53	55	55	57	57	57	57	57	57	57	57	57	57

I-28

TABLE  I-7 – CHAMBER TEMPERATURES AND HUMIDITIES (CONT’D)

CHAMBER HUMIDITIES (%RH) – 10,000 mg/m3 Target Concentration

	Time from Start of Exposure (Hours)

	0	0.5	1	1.5	2	2.5	3	3.5	4	4.5	5	5.5	6

27-Apr-02	71	57	57	57	57	57	57	57	57	57	57	57	57

28-Apr-02	57	50	51	51	51	51	51	51	51	51	51	51	51

29-Apr-02	64	57	51	51	51	42	42	42	42	42	42	42	42

30-Apr-02	64	64	57	57	57	57	57	57	57	57	57	57	57

1-May-02	64	54	51	51	45	45	45	45	46	49	49	49	49

2-May-02	64	61	58	58	58	58	58	58	46	46	46	46	46

3-May-02	64	57	51	52	52	52	46	46	46	46	46	46	46

4-May-02	61	65	58	58	58	58	58	58	58	58	58	58	58

5-May-02	64	57	51	52	52	52	52	52	52	52	52	52	52

6-May-02	90	77	58	58	59	59	59	59	59	53	53	53	53

7-May-02	64	65	59	59	59	59	59	53	53	53	53	53	53

8-May-02	72	65	58	56	56	56	56	56	56	56	56	56	56

9-May-02	64	65	58	58	58	58	58	53	53	53	53	53	53

10-May-02	64	53	54	56	56	56	53	53	53	53	53	53	53

11-May-02	64	65	52	52	53	53	53	53	53	53	53	53	53

12-May-02	72	66	59	59	59	59	59	59	59	59	59	59	59

13-May-02	72	73	59	59	53	53	53	53	53	60	60	60	60

14-May-02	64	50	44	45	45	45	45	45	45	46	46	45	45

15-May-02	71	57	58	52	52	52	52	53	53	52	52	52	52

16-May-02	64	57	51	51	51	45	45	52	52	52	52	52	52

17-May-02	64	57	51	51	58	58	52	52	52	52	52	52	52

18-May-02	62	57	53	47	45	45	45	45	45	45	52	52	52

19-May-02	71	64	58	58	58	58	58	58	58	58	58	58	58

20-May-02	62	57	57	57	51	51	51	51	51	51	51	51	51

21-May-02	53	57	50	44	44	44	44	44	45	45	45	45	45

I-29

TABLE  I-7 – CHAMBER TEMPERATURES AND HUMIDITIES (CONT’D)

CHAMBER HUMIDITIES (%RH) – 20,000 mg/m3 Target Concentration

	Time from Start of Exposure (Hours)

	0	0.5	1	1.5	2	2.5	3	3.5	4	4.5	5	5.5	6

27-Apr-02	62	64	64	64	57	57	57	57	57	57	57	57	57

28-Apr-02	57	57	57	57	57	57	57	57	57	57	57	57	57

29-Apr-02	55	57	57	57	57	57	57	57	57	57	57	57	57

30-Apr-02	71	64	57	57	57	57	57	57	57	57	57	57	57

1-May-02	57	57	57	57	57	57	57	57	51	51	51	51	51

2-May-02	72	64	64	64	64	57	57	57	58	58	58	58	58

3-May-02	64	57	64	57	57	57	51	51	51	58	58	58	58

4-May-02	71	64	57	57	57	57	57	51	51	51	51	51	51

5-May-02	57	64	57	57	57	57	57	57	57	57	57	57	57

6-May-02	90	90	77	69	58	58	58	58	58	58	58	58	58

7-May-02	64	72	57	65	65	58	58	58	58	58	58	58	58

8-May-02	72	72	65	65	58	58	58	58	58	58	58	58	58

9-May-02	62	64	57	57	57	57	57	65	65	65	65	65	65

10-May-02	71	72	72	65	58	58	58	58	58	58	58	58	58

11-May-02	71	72	65	58	58	56	59	52	52	52	52	59	59

12-May-02	64	69	66	66	66	66	66	66	66	66	66	66	66

13-May-02	72	72	65	58	58	58	58	58	58	58	59	59	59

14-May-02	55	57	50	50	50	44	44	44	44	51	51	51	51

15-May-02	62	64	57	57	51	51	51	51	51	51	51	51	51

16-May-02	64	57	57	57	57	50	50	50	50	50	50	57	57

17-May-02	64	57	57	57	57	57	57	57	57	57	57	57	57

18-May-02	62	55	57	57	57	50	50	50	50	50	50	57	57

19-May-02	62	64	57	57	57	57	57	57	57	57	57	57	57

20-May-02	62	64	57	57	57	57	57	57	57	57	57	57	57

21-May-02	53	55	55	57	57	57	57	57	50	50	50	50	50

I-30

	APPENDIX J - ANALYTICAL CHEMISTRY REPORT

	SUMMARY

Charcoal tube sorbent tube samples were received by the Analytical Chemistry Laboratory from  inhalation chamber exposures and were characterized for hydrocarbon distribution using capillary gas chromatography with flame ionization detection (GC/FID).  Sorbent tube samples were stored in a freezer pending analysis.

	SAMPLE PREPARATION

The front and back sections of each charcoal sample tube were desorbed and analyzed separately to

assess potential sampling breakthrough.  The charcoal tube sections were desorbed with 3.0 mL  carbon disulfide (CS2) for at least 30 minutes.  Aliquots were then analyzed by GC-FID.

	STANDARDIZATION

A standard mixture was prepared in CS2 containing each of the 18 target hydrocarbons plus ETBE oxygenate.  Analysis of the standard mixture was used to confirm the relative retention times of each target hydrocarbon and was not used for quantitative purposes.

CHARACTERIZATION

Characterization of the neat MRD-00-716 (gasoline vapor condensate with ETBE) was performed separately and will be reported as part of EMBSI Study 167490. Neat test substance characterization included a similar analysis of the relative distribution of target hydrocarbons and oxygenate as was performed for the chamber sorbent tube samples.

J-1

APPENDIX J - ANALYTICAL CHEMISTRY REPORT

INSTRUMENT CONDITIONS FOR MRD-00-716

ON CHARCOAL SORBENT TUBE

The following GC conditions and equipment were used to determine the hydrocarbon distribution of test substance on chamber characterization sorbent tubes:

GC	Perkin Elmer XL Autosystem

FID Range	FID (2^5)

Detector Temperature; (gas flows)	225°C (H2 45 mL/min; Air  450 mL/min)

Injector Temperature (Split ratio)	225°C (split injection 20:1)

Injection Volume	0.2µL or 0.5µL*

Analytical Column	Supleco Petrocol DH 150 fused silica capillary column (150m x 0.25mm; 1.0µm film thickness)

Oven Temperature Program	35(C (hold 130 minutes); ramp @ 2(C/minute to 200(C

Data Collection	Perkin Elmer Nelson Turbochrom (TC4) version 4.1.2

Carrier Gas Program	65 psi (He)

*0.2µL injected of 10,000mg/m3 and 20,000mg/m3 samples, 0.5µL injected of 2000mg/m3 samples.

RESULTS

Hydrocarbon characterization was performed on an area percent basis for each of the 18

target hydrocarbons and ETBE oxygenate. Results are listed in Table J-1.

The relative distribution of hydrocarbons and oxygenate measured on the chamber characterization charcoal tubes was in good agreement with that measured in the characterization of neat MRD-00-716 test substance.

D. J. Letinski						Date

Analytical Chemist

J-2

APPENDIX J - ANALYTICAL CHEMISTRY REPORT

TABLE J - 1

MRD-00-716 CHAMBER CHARACTERIZATION - SORBENT TUBES HYDROCARBON DISTRIBUTION

Sample Date	30-Apr-02	8-May-02	14-May-02	21-May-02

Inhalation ID	1	2	3	4	5	6	7	8	9	10	11	12

	 mg/m3	 mg/m3	 mg/m3	 mg/m3

	2000	10,000	20,000	2000	10,000	20,000	2000	10,000	20,000	2000	10,000 20,000

Compound	RESULTS   ARE   in   "AREA %"  of   TARGET   HYDROCARBONS

isobutane	1.7	1.5	1.7	1.8	1.7	1.6	1.7	1.7	1.7	1.7	1.6	1.7

n-butane	9.6	8.7	9.7	10.0	9.6	9.4	9.5	9.7	9.5	9.7	9.3	9.6

isopentane	31.8	32.1	32.6	32.5	32.7	32.6	32.2	32.3	32.6	32.4	32.1	33.1

n-pentane	9.8	10.0	9.9	9.9	10.0	10.0	9.8	9.9	10.0	9.9	9.9	10.1

trans-2-pentene	2.2	2.2	2.2	2.2	2.2	2.2	2.1	2.2	2.2	2.2	2.2	2.2

2-methyl-2-butene	3.2	3.3	3.2	3.2	3.2	3.2	3.2	3.2	3.2	3.2	3.2	3.3

2,3-dimethylbutane	1.7	1.5	1.7	1.5	1.5	1.5	1.7	1.7	1.5	1.5	1.7	1.5

2-methylpentane	4.9	5.0	4.9	4.8	4.9	4.9	4.9	4.9	4.9	4.9	4.9	4.9

3-methylpentane	3.1	3.2	3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1

n-hexane	2.6	2.7	2.6	2.6	2.6	2.6	2.6	2.6	2.6	2.6	2.6	2.6

ETBE	17.1	17.6	16.9	16.6	17.0	17.2	17.1	17.1	17.1	16.9	17.4	16.9

methylcyclopentane	1.4	1.4	1.3	1.3	1.4	1.4	1.4	1.4	1.4	1.3	1.4	1.3

2,4-dimethylpentane	1.1	1.2	1.1	1.1	1.1	1.1	1.1	1.1	1.1	1.1	1.1	1.1

benzene	2.2	2.0	1.9	2.2	1.9	1.9	2.2	1.9	1.9	2.2	1.9	1.9

2-methylhexane	1.2	1.3	1.2	1.2	1.2	1.2	1.2	1.2	1.2	1.2	1.2	1.1

2,3-dimethylpentane	1.3	1.3	1.2	1.2	1.2	1.2	1.2	1.2	1.3	1.2	1.3	1.2

3-methylhexane	1.4	1.4	1.4	1.4	1.3	1.4	1.4	1.3	1.4	1.4	1.4	1.3

isooctane	1.5	1.6	1.4	1.4	1.4	1.5	1.4	1.3	1.5	1.5	1.5	1.3

toluene	2.3	2.3	2.1	2.2	2.1	2.1	2.3	2.1	2.1	2.2	2.3	1.9

Sum	100	100	100	100	100	100	100	100	100	100	100	100

No hydrocarbon target compounds were detected on the back section of the sorbent tubes analyzed.

J-3

APPENDIX K – STATISTICIAN’S REPORT

Analysis Of Fetal Data From A Whole-Body Inhalation Developmental Toxicity

Study In Rats With Baseline Gasoline with ETBE Vapor Condensate (MRD-00-713)

This report details the statistical analysis of fetal body weight and anomaly data from ExxonMobil Study 171634.  The study was conducted to evaluate the potential developmental toxicity of Baseline Gasoline with ETBE Vapor Condensate (GEVC).  GEVC was administered via whole-body inhalation exposure to pregnant rats during the period of major organogenesis and fetal growth.  GEVC was administered by whole-body inhalation exposure to 25 confirmed-mated Crl: CD®(SD) IGSBR female rats at target doses of 0 (air control) 2000, 10,000, and 20,000 mg/m3 for six hours (plus the theoretical equilibration time) daily from Gestation Day (GD) 5 through GD 20.

The fetal body weight was analyzed by a mixed model analysis of variance that provided an accurate statistical model of the biology.  The analysis used the litter as the basis for analysis and effectively used the litter size as a covariate.  The model considered dose group, litter size, and fetal sex as explanatory variables.  When the overall effect of dose, or the dose by sex effect, was statistically significant the dose groups means were tested pairwise vs. the control group using least squares means.  The least squares means allowed comparisons that accounted for differences in litter size and sex.  The mathematical model is based on a paper by Chen, et al (1996).  The analysis was run using SAS with code suggested in Little, et al (1997).

The analysis of anomalies (malformations, variations, or observations) was based on a Generalized Estimating Equation (GEE) application of the linearized model, Ryan (1992).  The model used the litter as the basis for analysis and considered correlation among littermates by incorporating an estimated constant correlation and the litter size as a covariate.  When the overall effect of dose, or the dose by sex effect, was statistically significant the dose groups were tested pairwise vs. the control group using least squares means.  The least squares means allows comparisons that account for differences in litter size.  There were three categories of anomalies tested, and within each category specific anomalies were also tested.  In addition to the category specific anomalies a series of combined analyses were performed within each category:

Combined Malformations and Variations for All Fetuses

Combined Malformations and Variations for Alive Fetuses

Combined Malformations and Variations for Dead Fetuses

Malformations for All Fetuses

Malformations for Alive Fetuses

Malformations for Dead Fetuses

Variations for All Fetuses

Variations for Alive Fetuses

Variations for Dead Fetuses

In some cases there were no dead fetuses in a category.

K-1

APPENDIX K – STATISTICIAN’S REPORT (CONT’D)

Table I lists the four categories and corresponding sub-categories.  Within each category several subcategories were combined into a group of similar anomalies.  For example in the Category Skeletal the separate sub-categories of “SKELETAL/RIBS (C7): Cervical rib; Bilateral”, “SKELETAL/RIBS: Rudimentary supernumerary ribs”, and “SKELETAL/RIBS (L1):  Well-formed; Left” were combined into a sub-category “SKELETAL/RIBS:  supernumerary Ribs(combined)”.  In this combined category an animal is counted once when he, or she, exhibits more than one characteristic.  The sub-categories that were combined are listed in the Sub-categories Combined column of Table 1 by the sub-category number.  The analyses were run using SAS.

Table I

Anomaly Categories and Corresponding Sub-categories

Category	Sub-Category	Sub-categories combined

Head	1- HEAD:  Anolphthalmia; Bilateral

2- HEAD:  Dilated lateral ventricles; Bilateral

3- HEAD:  Open eye; Right

4- HEAD:  Retina fold

	External	1- EXTERNAL:  Acaudate

2- EXTERNAL:  Anal atresia

3- EXTERNAL:  Anasarca

4- EXTERNAL:  Brachydactyly; all paws

5- EXTERNAL:  Conjoined twin; Joined at thoracic region; 1 head, 8 limbs, 2 tails, 1 umbilicus

6- EXTERNAL:  Domed head

7- EXTERNAL:  Ectrodactyly; all paws

8- EXTERNAL:  Kyphosis

9- EXTERNAL:  Malrotated hindpaw; Left

10- EXTERNAL:  Small eye bulge; Bilateral

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APPENDIX K – STATISTICIAN’S REPORT (CONT’D)

Table I

Malformation Categories and Corresponding Sub-categories

Abdomen/Thorax	1- ABDOMEN/THORAX:  All other abdominal organs appear on each side

2- ABDOMEN/THORAX:  All thoracic and cranial organs shared

3- ABDOMEN/THORAX:  Cecum not evident; bifurcation appears in area where cecum would normally reside

4- ABDOMEN/THORAX:  Double aorta

5- ABDOMEN/THORAX:  Duplicate tongue

6- ABDOMEN/THORAX:  Elongated innominate artery

7- ABDOMEN/THORAX:  Enlarged atrial chamber; Right

8- ABDOMEN/THORAX:  Hydronephrosis

9- ABDOMEN/THORAX:  Hydroureter

10- ABDOMEN/THORAX:  Innominate artery absent

11- ABDOMEN/THORAX:  Liver, stomach, spleen, entire small intestine shared

12- ABDOMEN/THORAX:  Malpositioned carotid

13- ABDOMEN/THORAX:  Malpositioned carotid and subclavian branches; Bilateral

14- ABDOMEN/THORAX:  Malpositioned kidneys

15- ABDOMEN/THORAX:  Malpositioned ovaries

16- ABDOMEN/THORAX:  Malpositioned pulmonary artery branch

17- ABDOMEN/THORAX:  Malpositioned subclavian branch; Right

18- ABDOMEN/THORAX:  Malpositioned uterus

19- ABDOMEN/THORAX:  Misshapen spleen

20- ABDOMEN/THORAX:  No cervical spinal column

21- ABDOMEN/THORAX:  Spleen small

22- ABDOMEN/THORAX:  Supernumerary lung lobe; Left

23- ABDOMEN/THORAX:  Umbilical artery aneurysm

24- ABDOMEN/THORAX:  Umbilical artery arises from left side of urinary bladder

25- ABDOMEN/THORAX:  Ventricle small; Left

26- ABDOMEN/THORAX:  Abnormal spleen	19,21

	27- ABDOMEN/THORAX:  Abnormal thoracic/cervical blood vessels 6,10,13,12,16,17

	28- ABDOMEN/THORAX:  Malpositioned urogenital organs	14,15,18

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APPENDIX K – STATISTICIAN’S REPORT (CONT’D)

Table I

Malformation Categories and Corresponding Sub-categories

Skeletal	1- SKELETAL/FORELIMB (All bones ):  Duplication or extra bones

2- SKELETAL/FOREPAW (All bones):  Duplication or extra bones

3- SKELETAL/FOREPAW (Metacarpal 1 anlage):  Misshapen; Left

4- SKELETAL/FOREPAW (Proximal phalanges 2-4):  Unossified; Bilateral

5- SKELETAL/HINDLIMB (All bones):  Duplication or extra bones; Bilateral

6- SKELETAL/HINDPAW (All bones):  Duplication or extra bones; Bilateral

7- SKELETAL/HINDPAW (Calcaneus): Advanced; Bilateral

8- SKELETAL/PECTORAL GIRDLE (All bones):  Duplication or extra bones; Bilateral

9- SKELETAL/PELVIC GIRDLE (All bones):  Duplication or extra bones; Bilateral

10- SKELETAL/RIBS (All bones): Duplication or extra bones

11- SKELETAL/RIBS (C7 and T1 Anlage):  Fused; Anlage on C7 and T1 rib fused before it meets sternebrae

12- SKELETAL/RIBS (C7, C7 Anlage):  Cervical rib; Bilateral

13- SKELETAL/RIBS (L1): Well-formed; Left

14- SKELETAL/RIBS (Right twin T8 left, Left twin T8 right):  Fused; Left

15- SKELETAL/RIBS (T13):  Short last rib; Right

16- SKELETAL/RIBS:  Rudimentary supernumerary ribs

17- SKELETAL/SKULL (Interparietal):  Misshapen

18- SKELETAL/SKULL (Squamosal Process):  Hypoplastic; Left

19- SKELETAL/SKULL (Supraoccipital,Interparietal,Parietals,Frontals,Nasals):  Duplication or extra bone

20- SKELETAL/SKULL (Tympanics):  Hypoplastic

21- SKELETAL/STERNEBRAE (All bones):  Duplication or extra bones

22- SKELETAL/STERNEBRAE (VI):  Advanced

23- SKELETAL/STERNEBRAE:  Asymmetric sternebrae

24- SKELETAL/STERNEBRAE:  Bifid sternebrae

25- SKELETAL/STERNEBRAE:  Dumbbell-shaped sternebrae

26- SKELETAL/STERNEBRAE:  Hypoplastic sternebrae

27- SKELETAL/STERNEBRAE:  Misshapen sternebrae

28- SKELETAL/STERNEBRAE:  Unossified sternebrae

29- SKELETAL/VERTEBRAE (All bones):  Duplication or extra bones

30- SKELETAL/VERTEBRAE (CE3 Anlage):  Bifid centra

31- SKELETAL/VERTEBRAE (L): One less presacral vertebrae

32- SKELETAL/VERTEBRAE (L1):  Misshapen centra

33- SKELETAL/VERTEBRAE (Left twin CE 1-7):  Fused

34- SKELETAL/VERTEBRAE (Left twin T2):  Unossified centra

35- SKELETAL/VERTEBRAE (Left twin T3):  Hemicentra

36- SKELETAL/VERTEBRAE:  Bifid vertebral centra

37- SKELETAL/VERTEBRAE:  Dumbbell-shaped vertebral centra

38- SKELETAL/VERTEBRAE:  Dumbbell-shaped vertebral centra anlage

39- SKELETAL/RIBS:  Supernumerary ribs	13,17,14

	40- SKELETAL/STERNEBRAE:  Hypoplastic sternebrae	24,25,26,28

	41- SKELETAL/VERTEBRAE:  Hypoplastic vertebral centra	35,36,37,38

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APPENDIX K – STATISTICIAN’S REPORT (CONT’D)

RESULTS:

BODY WEIGHT ANALYSIS

There was no statistically significant difference in the mean fetal litter weights among the dose groups.  Table II shows the mean fetal weight and the least squares mean fetal weight.  The weight difference between the male and female pups was not consistent across the dose groups; therefore only the mean pup weight respective of sex is presented.

Table II

Mean fetal weight, the least squares mean fetal weight

Exposure Group

(mg/m3)	n litters 	n fetuses	observed fetus mean (gm)	Least squares fetus mean (gm)

0	25	353	5.32	5.33

2,000	23	329	5.31	5.34

10,000	24	325	5.30	5.29