________ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN EPIDIDYMIDES TESTES THYMUS ADRENAL GLANDS THYROIDS/ PARA FINAL BODY WT( G)   _    _ _ _ _ ._____ ABS.( G) REL. 1.95 0.586 8.96 2.691 2.24 0.673 1.25 0.375 1.01 0.303 0.70 0.210 1.08 0.324 3.22 0.967 0.2108 0.063 0.0601 0.018 0.0163 333. 0.005 40 PPM MALE SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE ___________________ NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECTJM COLON DUODENUM LAC GLAND, EXOR EPIDIDYMIDES ESOPHAGUS EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM JEJUNUM KIDNEYS LIVER AORTA LUNGS LARYNX NERVE, SCIATIC LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES PANCREAS TESTES THYMUS RECTUM PITUITARY PROSTATE SAL. GLAND MAND SEMINAL VESICLES SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER GROSS GRADE CODE: l SLIGHT, I MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 26 ANIMAL NO. 69328 GROUP 3: 40 PPM MALE SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE ____ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN EPIDIDYMIDES TESTES THYMUS ADRENAL GLANDS THYROIDS/ pARA FINAL BODY WT( G) ABS.( G) REL. SPLEEN 1.90 0.590 8.59 2.668 NO SIGNIFICANT 2.20 0.683 CHANGES OBSERVED 1.30 0.404 1.06 0.329 0.63 0.196 1.08 0.335 3.95 1.227 GROSS: ACCESSORY P ONE, 3 MM IN DIAMETER GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM COLON DUODENUM LAC GLAND, EXOR EPIDIDYMIDES ESOPHAGUS EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM JEJUNUM KIDNEYS LIVER AORTA LUNGS LARYNX NERVE, SCIATIC LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES PANCREAS THYMUS RECTUM PITUITARY 0.2050 0.064 TESTES 0.0478 0.015 PROSTATE 0.0163 0.005 SKIN 322. TRACHEA GROSS GRADE CODE: SAL. GLAND MAND SEMINAL VESICLES SKELETAL MUSCLE SPINAL CORD STOMACH THYROID GLANDS URINARY BLADDER l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 9O DAY INHAL. STUDY OF l, l, Z TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 27 ANIMAL NO. 69339 GROUP 3: 40 PPM MALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN EPIDIDYMIDES TESTES THYMUS ADRENAL GLANDS THYROIDSjPARA FINAL BODY WT( G) ABS.( G) REL. 1.77 0.656 7.44 2.756 1.94 0.719 0.90 0.333 0.91 0.337 0.51 0.189 0.88 0.326 2.80 1.037 0.1799 0.0572 0.0165 270. 0.067 0.021 0.006 NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM COLON DUODENUM ESOPHAGUS EYES/ OPTIC N. ILEUM JEJUNUM AORTA LUNGS LYMPH NODE, TR/ BR LYMPH NODE, MED TESTES THYMUS PROSTATE SAL. GLAND MAND SKIN SPINAL CORD THYROID GLANDS TRACHEA BRAIN CECUM LAC GLAND, EXOR EPIDIDYMIDES HARDERIAN GLANDS HEART KIDNEYS LIVER LARYNX NERVE, SCIATIC NASAL TISSUES PANCREAS RECTUM PITUITARY SEMINAL VESICLES SKELETAL MUSCLE SPLEEN STOMACH URINARY BLADDER GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 28 ANIMAL NO. 69344 GROUP 3: 40 PPM MALE _______________________ ORGAN WEIGHT ABS.( G) BRAIN 1.84 LIVER 9.00 KIDNEYS 2.32 LUNGS 1.24 HEART 1.01 SPLEEN 0.68 EPIDIDYMIDES 0.99 TESTES 3.11 THYMUS 0.2125 ADRENAL GLANDS 0.0562 THYROIDS/ PARA 0.0148 FINAL BODY WT( G) 316. SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE REL. NO SIGNIFICANT 0.582 CHANGES OBSERVED 2.848 0.734 0.392 0.320 0.215 0.313 0.984 0.067 0.018 0.005 GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM COLON DUODENUM LAC GLAND. EXOR EPIDIDYMIDES ESOPHAGUS ILEUM AORTA LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES PANCREAS TESTES THYMUS RECTUM PITUITARY EYES/ OPTIC N. HARDERIAN'GLANDS HEART JEJUNUM KIDNEYS LIVER LUNGS LARYNX NERVEsSCIATIC PROSTATE SAL. GLAND MAND SEMINAL VESICLES SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER GROSS GRADE CODE: l SLIGHT, Z MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 29 ANIMAL NO. 69348 GROUP 3: 40 PPM MALE _____________ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN EPIDIDYMIDES TESTES THYMUS ADRENAL GLANDS THYROIDS/ PARA FINAL BODY WT( G) ABS.( G) 1.92 8.36 2.17 1.19 0.95 0.60 0.99 3.16 0.1814 0.0565 0.0236 292. ________________. REL. STOMACH 0.658 NO SIGNIFICANT 2.863 CHANGES OBSERVED 0.743 0.408 0.325 0.205 0.339 1.082 0.062 0.019 0.008 SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE GROSS: CONTENTS, DARK RED P GROSS: ADRENAL GLANDS STERNUM COLON DUODENUM ESOPHAGUS EYES/ OPTIC N. ILEUM JEJUNUM AORTA LUNGS LYMPH NODE, TR/ BR LYMPH NODE, MED TESTES THYMUS PROSTATE SAL. GLAND MAND SKIN SPINAL CORD TRACHEA URINARY BLADDER BRAIN CECUM LAC GLAND, EXOR EPIDIDYMIDES HARDERIAN GLANDS HEART KIDNEYS LIVER LARYNX NERVE, SCIATIC NASAL TISSUES PANCREAS RECTUM PITUITARY SEMINAL VESICLES SKELETAL MUSCLE SPLEEN THYROID GLANDS GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 30 ANIMAL NO. 69350 GROUP 3: 40 PPM MALE _________________ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN EPIDIDYMIDES TESTES THYMUS ADRENAL GLANDS THYROIDS/ pARA FINAL BODY WT( G) .______ ABS. (G) 1.88 7.28 1.95 1.20 0.92 0.61 0.87 3.00 0.2170 0.0566 0.0128 280. _______________________ REL. NO SIGNIFICANT 0.671 CHANGES OBSERVED 2.600 0.696 0.429 0.329 0.218 0.311 1.071 0.077 0.020 0.005 SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM COLON DUODENUM LAC GLAND, EXOR EPIDIDYMIDES ESOPHAGUS EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM JEJUNUM KIDNEYS LIVER AORTA LUNGS LARYNX NERVE, SCIATIC LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES PANCREAS TESTES THYMUS RECTUM PITUITARY PROSTATE SAL. GLAND MAND SEMINAL VESICLES SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 31 ANIMAL NO. 69304 GROUP 4: 100 PPM MALE ________________ ________ ORGAN WEIGHT ABS.( G) BRAIN 1.91 LIVER 8.98 KIDNEYS 2.36 LUNGS 1.30 HEART 1.07 SPLEEN 0.76 EPIDIDYMIDES 1.09 TESTES 3.50 THYMUS 0.2438 ADRENAL GLANDS 0.0674 THYROIDS/ PAPA 0.0140 FINAL BODY WT( G) 323. _________________________ REL. THYMUS 0.591 NO SIGNIFICANT 2.780 CHANGES OBSERVED 0.731 0.402 0.331 0.235 0.337 1.084 0.075 0.021 0.004 SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE _______________ GROSS: DISCOLORATION, DARK RED P GROSS: ADRENAL GLANDS COLON ESOPHAGUS ILEUM AORTA LYMPH NODE, TR/ BR TESTES SAL. GLAND MAND SPINAL CORD TRACHEA STERNUM BRAIN DUODENUM LAC GLAND, EXOR EYES/ OPTIC N. HARDERIAN GLANDS JEJUNUM KIDNEYS LUNGS LARYNX LYMPH NODE, MED NASAL TISSUES RECTUM PITUITARY SEMINAL VESICLES SKELETAL MUSCLE SPLEEN STOMACH URINARY BLADDER CECUM EPIDIDYMIDES HEART LIVER NERVE, SCIATIC PANCREAS PROSTATE SKIN THYROID GLANDS GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 32 ANIMAL NO. 69307 GROUP 4: 100 PPM MALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN EPIDIDYMIDES TESTES THYMUS ADRENAL GLANDS THYROIDS/ PARA FINAL BODY WT( G) ABS.( G) REL. 1.95 0.596 9.49 2.902 2.22 0.679 1.36 1.13 0.65 1.06 3.31 0.2473 0.0637 0.0197 327. 0.416 0.346 0.199 0.324 1.012 0.076 0.019 0.006 LUNGS GROSS: AREA( S), WHITE FEW, IRREGULARLY SHAPED, ALL SKIN GROSS: MATTING, RED OCULAR, RIGHT NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM COLON DUODENUM ESOPHAGUS EYES/ OPTIC N. ILEUM JEJUNUM AORTA LARYNX LYMPH NODE, MED NASAL TISSUES THYMUS RECTUM P LOBES P BRAIN CECUM LAC GLAND, EXOR EPIDIDYMIDES HARDERIAN GLANDS HEART KIDNEYS LIVER NERVE, SCIATIC LYMPH NODE, TR/ BR PANCREAS TESTES PITUITARY PROSTATE SAL. GLAND MAND SEMINAL VESICLES SKELETAL MUSCLE SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT TABLE 31 (PRIMARY NECROPSY) PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE SPONSOR: HAP TASK FORCE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 33 ANIMAL NO. 69318 GROUP 4: 100 PPM MALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 ____________ ________. ORGAN WEIGHT ABS.( G) REL. BRAIN 1.83 0.594 LIVER 8.70 2.825 KIDNEYS 2.15 0.698 LUNGS 1.21 0.393 HEART 0.99 0.321 SPLEEN 0.64 0.208 EPIDIDYMIDES 0.95 0.308 TESTES 3.09 1.003 THYMUS 0.2204 0.072 ADRENAL GLANDS 0.0538 0.017 THYROIDS/ PARA 0.0228 0.007 FINAL BODY WT( G) 308. NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM COLON DUODENUM ESOPHAGUS EYES/ OPTIC N. ILEUM JEJUNUM AORTA LUNGS LYMPH NODE, TR/ BR LYMPH NODE, MED TESTES THYMUS PROSTATE SAL. GLAND MAND SKIN SPINAL CORD THYROID GLANDS TRACHEA I GRADE _____________________________

BRAIN CECUM LAC GLAND, EXOR EPIDIDYMIDES HARDERIAN GLANDS HEART KIDNEYS LIVER LARYNX NERVE, SCIATIC NASAL TISSUES PANCREAS RECTUM PITUITARY SEMINAL VESICLES SKELETAL MUSCLE SPLEEN STOMACH URINARY BLADDER GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 34 ANIMAL NO. 69319 GROUP 4: 100 PPM MALE SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN EPIDIDYMIDES TESTES ADRENAL GLANDS THYROIDS/ PAR?+ FINAL BODY WT( G) ABS.( G) 1.87 8.46 2.19 1.27 0.94 0.66 1.01 3.30 0.1970 0.0508 0.0152 305. REL. 0.613 2.774 0.718 0.416 0.308 0.216 0.331 1.082 0.065 0.017 0.005 NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS COLON ESOPHAGUS ILEUM AORTA LYMPH NODE, TR/ BR TESTES PROSTATE SKIN THYROID GLANDS STERNUM BRAIN CECUM DUODENUM LAC GLAND, EXOR EPIDIDYMIDES EYES/ OPTIC N. HARDERIAN GLANDS HEART JEJUNUM KIDNEYS LIVER LUNGS LARYNX NERVE, SCIATIC LYMPH NODE, MED NASAL TISSUES PANCREAS THYMUS RECTUM PITUITARY SAL. GLAND MAND SEMINAL VESICLES SKELETAL MUSCLE SPINAL CORD SPLEEN STOMACH TRACHEA URINARY BLADDER GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 35 ANIMAL NO. 69327 GROUP 4: 100 PPM MALE SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE ORGAN WEIGHT ABS. (G) BRAIN 1.84 LIVER 7.71 KIDNEYS 2.01 LUNGS 1.22 HEART 0.92 SPLEEN 0.64 EPIDIDYMIDES 0.98 TESTES 3.08 THYMUS 0.1762 ADRENAL GLANDS 0.0472 THYROIDS/ PARA 0.0166 FINAL BODY WT( G) 303. REL. 0.607 2.564 0.663 0.403 0.304 0.211 0.323 1.017 0.058 0.016 0.005 NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS COLON ESOPHAGUS ILEUM AORTA LYMPH NODE, TR/ BR TESTES PROSTATE SKIN THYROID GLANDS STERNUM DUODENUM EYES/ OPTIC N. JEJUNUM LUNGS LYMPH NODE, MED THYMUS SAL. GLAND MAND SPINAL CORD TRACHEA BRAIN CECUM LAC GLAND, EXOR EPIDIDYMIDES HARDERIAN GLANDS HEART KIDNEYS LIVER LARYNX NERVE, SCIATIC NASAL TISSUES PANCREAS RECTUM PITUITARY SEMINAL VESICLES SKELETAL MUSCLE SPLEEN STOMACH URINARY BLADDER GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT ANIMAL NO. 69329 GROUP _____________. ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN EPIDIDYMIDES TESTES THYMUS ADRENAL GLANDS THYROIDS/ PARA FINAL BODY WT( G) ___ _____. ABS.( G) 1.87 8.34 2.21 1.21 0.95 0.62 1.01 2.98 0.1926 0.0536 0.0224 293, TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 36 4: 100 PPM MALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE REL. 0.638 2.846 0.754 0.413 0.324 0.212 0.345 1.017 0.066 0.018 0.008 ________________________________________ SKIN GROSS: MATTING, RED OCULAR, RIGHT NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM COLON DUODENUM ESOPHAGUS EYES/ OPTIC N. ILEUM JEJUNUM AORTA LUNGS LYMPH NODE, TR/ BR LYMPH NODE, MED TESTES THYMUS PROSTATE SAL. GLAND MAND SPINAL CORD SPLEEN TRACHEA URINARY BLADDER BRAIN LAC GLAND, EXOR HARDERIAN GLANDS KIDNEYS LARYNX NASAL TISSUES RECTUM SEMINAL VESICLES STOMACH P CECUM EPIDIDYMIDES HEART LIVER NERVE, SCIATIC PANCREAS PITUITARY SKELETAL MUSCLE THYROID GLANDS GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) PO DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 37 ANIMAL NO. 69332 GROUP 4: 100 PPM MALE SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE ____________ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN EPIDIDYMIDES TESTES THYMUS ADRENAL GLANDS THYROIDS/ PARA FINAL BODY WT( G) ABS. (G) 1.90 7.56 1.94 1.11 0.97 0.56 1.11 3.05 0.1944 0.0499 0.0150 293. ______ REL. 0.648 2.580 0.662 0.379 0.331 0.191 0.379 1.041 0.066 0.017 0.005 ____________________________________ NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM COLON DUODENUM LAC GLAND, EXOR EPIDIDYMIDES ESOPHAGUS EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM JEJUNUM KIDNEYS LIVER AORTA LUNGS LARYNX NERVE, SCIATIC LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES PANCREAS TESTES THYMUS RECTUM PITUITARY PROSTATE SAL. GLAND MAND SEMINAL VESICLES SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT TABLE 31 (PRIMARY NECROPSY) PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE SPONSOR: HAP TASK FORCE INDIVIDUAL MACROSCOPIC FINDINGS ANIMAL NO. 69334 GROUP 4: 100 PPM MALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 9l. GRADE PAGE 38 ORGAN WEIGHT ABS. (G) BRAIN 1.82 LIVER 8.48 KIDNEYS 2.17 LUNGS 1.19 HEART 0.97 SPLEEN 0.60 EPIDIDYMIDES 0.97 TESTES 3.06 THYMUS 0.1969 ADRENAL GLANDS 0.0572 THYROIDSjPARA 0.0225 FINAL BODY WT( G) 288. REL. NO SIGNIFICANT 0.632 CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM 2.944 COLON DUODENUM 0.753 ESOPHAGUS EYES/ OPTIC N. 0.413 ILEUM JEJUNUM 0.337 AORTA LUNGS 0.208 LYMPH NODE, TR/ BR LYMPH NODE, MED 0.337 TESTES THYMUS 1.063 0.068 0.020 0.008 PROSTATE SAL. GLAND MAND SKIN SPINAL CORD THYROID GLANDS TRACHEA BRAIN CECUM LAC GLAND, EXOR EPIDIDYMIDES HARDERIAN GLANDS HEART KIDNEYS LIVER LARYNX NERVE, SCIATIC NASAL TISSUES PANCREAS RECTUM PITUITARY SEMINAL VESICLES SKELETAL MUSCLE SPLEEN STOMACH URINARY BLADDER GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 39 ANIMAL NO. DATE OF DEATH: 08/ 31/ 01 STUDY DAY:

ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN EPIDIDYMIDES TESTES THYMUS ADRENAL GLANDS THYROIDS/ PARA FINAL BODY WT( G) ABS.( G) 1.79 8.46 2.22 1.14 0.88 0.55 0.96 3.01 0.1683 0.0562 0.0168 306. REL. 0.585 2.765 0.725 0.373 0.288 0.180 0.314 0.984 0.055 0.018 0.005 GENERAL COMMENT GROSS: ORGAN DAMAGED AT NECROPSY PITUITARY NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM COLON DUODENUM ESOPHAGUS EYES/ OPTIC N. ILEUM JEJUNUM AORTA LUNGS LYMPH NODE, TR/ BR LYMPH NODE, MED TESTES THYMUS PROSTATE SAL. GLAND MAND SKIN SPINAL CORD THYROID GLANDS TRACHEA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 BRAIN CECUM LAC GLAND, EXOR EPIDIDYMIDES HARDERIAN GLANDS HEART KIDNEYS LIVER LARYNX NERVE, SCIATIC NASAL TISSUES PANCREAS RECTUM PITUITARY SEMINAL VESICLES SKELETAL MUSCLE SPLEEN STOMACH URINARY BLADDER MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 40 ANIMAL NO. 69346 GROUP 4: 100 PPM MALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE ____________________________________~~~~~ ORGAN WEIGHT ABS. (G) REL. EYES/ OPTIC N. GROSS: EYE( S) OPACITY P BRAIN 1.78 0.587 LEFT LIVER 8.89 2.934 GENERAL COMMENT GROSS: ORGAN LOST AT NECROPSY P KIDNEYS 2.24 0.739 HEART LUNGS 1.19 0.393 NO SIGNIFICANT SPLEEN 0.57 0.188 CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM EPIDIDYMIDES 0.97 0.320 COLON DUODENUM LAC GLAND, EXOR EPIDIDYMIDES TESTES 3.00 0.990 ESOPHAGUS HARDERIAN GLANDS HEART ILEUM THYMUS 0.1742 0.057 JEJUNUM KIDNEYS LIVER AORTA ADRENAL GLANDS 0.0533 0.018 LUNGS LARYNX NERVE, SCIATIC LYMPH NODE, TR/ BR THYROIDS/ PARA 0.0159 0.005 LYMPH NODE, MED NASAL TISSUES PANCREAS TESTES FINAL BODY MT( G) 303. THYMUS RECTUM PITUITARY PROSTATE SAL. GLAND MAND SEMINAL VESICLES SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: KAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 41 ANIMAL NO. 69354 GROUP _________ ______ _ ___. ORGAN WEIGHT ABS.( G) BRAIN 1.90 LIVER 5.34 KIDNEYS 1.35 LUNGS 0.97 HEART 0.65 SPLEEN 0.49 OVARIES 0.0923 1: 0 PPM FEMALE SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE ______________________________________~~ REL. NO SIGNIFICANT 0.969 CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM 2.724 COLON DUODENUM LAC GLAND, EXOR ESOPHAGUS 0.689 EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM 0.495 JEJUNUM KIDNEYS LIVER AORTA 0.332 LUNGS MAMMARY GLAND LARYNX NERVE, SCIATIC 0.250 LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES OVARIES 0.047 PANCREAS OVIDUCTS THYMUS RECTUM THYMUS 0.2110 0.108 PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN ADRENAL GLANDS 0.0645 0.033 SPINAL CORD SPLEEN STOMACH THYROID GLANDS THYROIDS/ PARA 0.0128 0.007 TRACHEA URINARY BLADDER UTERUS VAGINA FINAL BODY WT( G) 196.

GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 42 ANIMAL NO. 69366 GROUP _________ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN OVARIES THYMUS ADRENAL GLANDS THYROIDS/ PARA FINAL BODY WT( G) ABS.( G) 1.71 4.41 1.27 0.93 0.59 0.44 0.0885 0.1814 0.0493 0.0131 179. 1:

______ REL. 0.955 2.464 0.709 0.520 0.330 0.246 0.049 0.101 0.028 0.007 0 PPM FEMALE SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE ________________________________________~~~~~~ NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM COLON DUODENUM LAC GLAND, EXOR ESOPHAGUS EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM JEJUNUM KIDNEYS LIVER AORTA LUNGS MAMMARY GLAND LARYNX NERVE, SCIATIC LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES OVARIES PANCREAS OVIDUCTS THYMUS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 43 ANIMAL NO.

ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN OVARIES 69367 GROUP 1: 0 PPM FEMALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE _

ABS. (G) REL. EYES/ OPTIC N. GROSS: EYE( S) OPACITY P 1.79 0.913 RIGHT 4.64 2.367 LYMPH NODE, MAN GROSS: DISCOLORATION, DARK RED P 1.36 0.694 RIGHT 0.96 0.490 NO SIGNIFICANT 0.62 0.316 CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM 0.52 0.265 COLON DUODENUM LAC GLAND, EXOR ESOPHAGUS 0.0744 0.038 HARDERIAN GLANDS HEART ILEUM JEJUNUM THYMUS 0.2682 0.137 KIDNEYS LIVER AORTA LUNGS ADRENAL GLANDS 0.0457 0.023 MAMMARY GLAND LARYNX NERVE, SCIATIC LYMPH NODE, TR/ BR THYROIDS/ PARA 0.0216 0.011 LYMPH NODE, MED NASAL TISSUES OVARIES PANCREAS FINAL BODY WT( G) 196. OVIDUCTS THYMUS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 44 ANIMAL NO, 69378 GROUP ORGAN WEIGHT ABS.( G) BRAIN 1.81 LIVER 4.86 KIDNEYS 1.27 LUNGS 1.03 HEART 0.65 SPLEEN 0.44 OVARIES 0.0969 THYMUS 0.2174 ADRENAL GLANDS THYROIDS/ PARA FINAL BODY WT( G) 0.0449 0.0109 180. 1: 0 PPM FEMALE SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE _____________________________________~~~ REL. NO SIGNIFICANT 1.006 CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM 2.700 COLON DUODENUM LAC GLAND. EXOR ESOPHAGUS 0.706 EYES/ OPTIC N. HARDERIAN GLANDS HEART ' ILEUM 0.572 JEJUNUM KIDNEYS LIVER AORTA 0.361 LUNGS MAMMARY GLAND LARYNX NERVE, SCIATIC 0.244 LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES OVARIES 0.054 PANCREAS OVIDUCTS THYMUS RECTUM 0.121 PITUITARY SAL, GLAND MAND SKELETAL MUSCLE SKIN 0.025 SPINAL CORD SPLEEN STOMACH THYROID GLANDS 0.006 TRACHEA URINARY BLADDER UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT TABLE 31 (PRIMARY NECROPSY) PROJECT NO.: WIL 417OQ2 SPONSOR: HAP TASK FORCE ANIMAL NO. 69384 GROUP _________ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN OVARIES THYMUS ADRENAL GLANDS THYROIDS/ PARA FINAL BODY WT( G) ABS.( G) 1.72 4.70 1.14 0.95 0.60 0.44 0.1006 0.1931 0.115 PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN 0.0491 0.029 SPINAL CORD SPLEEN STOMACH THYROID GLANDS 0.0163 168. 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 45 1: 0 PPM FEMALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE REL. NO SIGNIFICANT 1.024 CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM 2.798 COLON DUODENUM LAC GLAND, EXOR ESOPHAGUS 0.679 EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM 0.565 JEJUNUM KIDNEYS LIVER AORTA 0.357 LUNGS MAMMARY GLAND LARYNX NERVE, SCIATIC 0.262 LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES OVARIES 0.060 PANCREAS OVIDUCTS THYMUS RECTUM 0.010 TRACHEA URINARY BLADDER UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT TABLE 31 (PRIMARY NECROPSY) PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE SPONSOR: HAP TASK FORCE PAGE 46 INDIVIDUAL MACROSCOPIC FINDINGS ANIMAL NO. 69398 GROUP 1: 0 PPM FEMALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE ____________________________________ ORGAN WEIGHT ABS. (G) REL. NO SIGNIFICANT BRAIN 1.86 0.944 CHANGES OBSERVED GROSS: ADRENAL GLANDS LIVER STERNUM BRAIN 4.72 2.396 CECUM COLON KIDNEYS DUODENUM 1.32 0.670 LAC GLAND, EXOR ESOPHAGUS LUNGS EYES/ OPTIC N. 0.91 0.462 HARDERIAN GLANDS HEART ILEUM JEJUNUM HEART 0.68 KIDNEYS 0.345 LIVER AORTA LUNGS MAMMARY GLAND SPLEEN LARYNX NERVE, SCIATIC 0.48 0.244 OVARIES LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES 0.1011 0.051 OVARIES PANCREAS OVIDUCTS THYMUS 0.2156 THYMUS 0.109 RECTUM PITUITARY ADRENAL GLANDS 0.0639 0.032 SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN THYROIDS/ PARA 0.0155 STOMACH 0.008 THYROID GLANDS TRACHEA FINAL BODY WT( G) URINARY BLADDER UTERUS 197. VAGINA PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE ANIMAL NO. 69404 GROUP ._. ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN OVARIES THYMUS ADRENAL GLANDS THYROIDS/ PAR?+ FINAL BODY WT( G) _ _ _ _ _ _ _ _ ABS.( G) 1.81 4.81 1.40 0.99 0.68 0.50 0.1018 0.2587 0.0567 0.0127 199. TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 47 1:

  _ _ _ _ _ REL. 0.910 2.417 0.704 0.497 0.342 0.251 0.051 0.130 0.028 0.006 0 PPM FEMALE SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE ______________________________________ NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM COLON DUODENUM LAC GLAND, EXOR ESOPHAGUS EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM JEJUNUM KIDNEYS LIVER AORTA LUNGS MAMMARY GLAND LARYNX NERVE, SCIATIC LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES OVARIES PANCREAS OVIDUCTS THYMUS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NE~ ROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 48 ANIMAL NO. 69416 GROUP 1: 0 ____________ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN OVARIES THYMUS ADRENAL GLANDS THyROIDS/ PARA FINAL BODY WT( G) ABS. (G) 1.84 5.16 1.40 0.98 0.67 0.50 0.1029 0.2011 0.0554 0.0181 192. _ _ _ _     REL. 0.958 2.687 0.729 0.510 0.349 0.260 0.054 0.105 0.029 0.009 PPM FEMALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE ____________________________________~~~~ NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM COLON DUODENUM LAC GLAND, EXOR ESOPHAGUS EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM JEJUNUM KIDNEYS LIVER AORTA LUNGS MAMMARY GLAND LARYNX NERVE, SCIATIC LYMPH NODE. TR/ BR LYMPH NODE. MED NASAL TISSUES OVARIES PANCREAS ' OVIDUCTS ' THYMUS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH TRACHEA URINARY BLADDER UTERUS THYROID GLANDS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 49 ANIMAL NO. 69419 GROUP 1: 0 PPM ORGAN WEIGHT ABS.( G) BRAIN 1.76 LIVER 4.90 KIDNEYS 1.31 LUNGS 0.96 HEART 0.70 SPLEEN 0.48 OVARIES 0.1059 THYMUS 0.2328 ADRENAL GLANDS 0.0506 THYROIDS/ PARA 0.0161 FINAL BODY WT( G) 193. FEMALE REL. NO SIGNIFICANT 0.912 CHANGES OBSERVED 2.539 0.679 0.497 0.363 0.249 0.055 0.121 0.026 0.008 SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE ._____________________________________

GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM COLON DUODENUM LAC GLAND, EXOR ESOPHAGUS EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM JEJUNUM KIDNEYS LIVER AORTA LUNGS MAMMARY GLAND LARYNX NERVE, SCIATIC LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES OVARIES PANCREAS OVIDUCTS THYMUS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 50 ANIMAL NO. 69432 GROUP 1: 0 PPM FEMALE SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE _____________________________________ ORGAN WEIGHT ABS.( G) REL. NO SIGNIFICANT BRAIN 1.81 1.011 CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM LIVER 4.93 KIDNEYS 1.34 LUNGS 0.94 HEART 0.68 SPLEEN 0.51 OVARIES 0.0924 THYMUS 0.1837 ADRENAL GLANDS 0.0562 THYROIDS/ PARA 0.0132 FINAL BODY WT( G) 179. 2 0 0 0 0 0 0 754 749 525 380 285 052 103 031 0 0.007 COLON DUODENUM EYES/ OPTIC N. HARDERIAN GLANDS JEJUNUM KIDNEYS LUNGS MAMMARY GLAND LYMPH NODE, TR/ BR LYMPH NODE, MED PANCREAS OVIDUCTS PITUITARY SAL. GLAND MAND SPINAL CORD SPLEEN TRACHEA URINARY BLADDER LAC GLAND, EXOR HEART LIVER LARYNX NASAL TISSUES THYMUS SKELETAL MUSCLE STOMACH UTERUS ESOPHAGUS ILEUM AORTA NERVE, SCIATIC OVARIES RECTUM SKIN THYROID GLANDS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 TABLE 31 (PRIMARY NEcROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE SPONSOR: HAP TASK FORCE INDIVIDUAL MACROSCOPIC FINDINGS ANIMAL NO. 69362 GROUP 2:

ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN OVARIES THYMUS ADRENAL GLANDS THYROIDS/ PARA FINAL BODY NT( G) ABS. (G) 1.66 4.79 1.36 1.10 0.66 0.53 0.1351 0.2288 0.0687 0.0103 176. REL. 0.943 2.722 0.773 0.625 0.375 0.301 0.077 0.130 0.039 0.006 PAGE 51 15 PPM FEMALE SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE ________________ LYMPH NODE, MAN GROSS: DISCOLORATION, DARK RED P BILATERAL NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM COLON DUODENUM LAC GLAND, EXOR ESOPHAGUS EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM JEJUNUM KIDNEYS LIVER AORTA LUNGS MAMMARY GLAND LARYNX NERVE, SCIATIC LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES OVARIES PANCREAS OVIDUCTS THYMUS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 52 ANIMAL NO. 69376 GROUP _____________ ORGAN WEIGHT ABS.( G) BRAIN 1.71 LIVER 4.49 KIDNEYS 1.15 LUNGS 0.80 HEART il. 60 SPLEEN 0.42 OVARIES 0.0985 THYMUS 0.1878 ADRENAL GLANDS 0.0558 THYROIDS/ PARA 0.0128 FINAL BODY WT( G) 159, 2:

REL. 1.075 2.824 0.723 0.503 0.377 0.264 0.062 0.118 0.035 0.008 15 PPM FEMALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE ______________________________________~~~~~~ NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM COLON DUODENUM LAC GLAND, EXOR ESOPHAGUS EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM JEJUNUM KIDNEYS LIVER AORTA LUNGS MAMMARY GLAND LARYNX NERVE, SCIATIC LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES OVARIES PANCREAS OVIDUCTS THYMUS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 53 ANIMAL NO. 69382 GROUP 2: 15 PPM FEMALE SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE ______________________________________

ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN OVARIES THYMUS ADRENAL GLANDS THYROIDS/ PARA FINAL BODY WT( G) ABS. (G) 1.79 4.64 1.27 0.93 0.65 0.46 0.0997 0.1678 0.0590 0.0129 195. REL. 0.918 2.379 0.651 0.477 0.333 0.236 0.051 0.086 0.030 0.007 NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN COLON DUODENUM LAC GLAND, EXOR EYES/ OPTIC N. HARDERIAN GLANDS HEART JEJUNUM KIDNEYS LIVER LUNGS MAMMARY GLAND LARYNX LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES PANCREAS OVIDUCTS THYMUS PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SPINAL CORD SPLEEN STOMACH TRACHEA URINARY BLADDER UTERUS GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT CECUM ESOPHAGUS ILEUM AORTA NERVE, SCIATIC OVARIES RECTUM SKIN THYROID GLANDS VAGINA PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) $O DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 54 ANIMAL NO. 69390 GROUP 2: 15 PPM FEMALE SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE ________~ ORGAN WEIGHT ABS.( Gf REL. LUNGS GROSS: AREA( S), WHITE P BRAIN 1.74 0.956 FEW, IRREGULARLY SHAPED, ALL LOBES LIVER 4.42 2.429 SKIN GROSS: MATTING, YELLOW P KIDNEYS 1.37 0.753 UROGENITAL LUNGS 1.04 0.571 NO SIGNIFICANT HEART 0.65 0.357 CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM SPLEEN 0.51 0.280 COLON DUODENUM LAC GLAND, EXOR ESOPHAGUS OVARIES 0.0858 0.047 EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM THYMUS 0.2184 0.120 JEJUNUM KIDNEYS LIVER AORTA ADRENAL GLANDS 0.0564 0.031 MAMMARY GLAND LARYNX NERVE, SCIATIC LYMPH NODE, TR/ BR THYROIDS/ PARA 0.0126 0.007 LYMPH NODE, MED NASAL TISSUES OVARIES PANCREAS FINAL BODY WT( G) 182. OVIDUCTS THYMUS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 90 DAY SPONSOR: HAP TASK FORCE ANIMAL NO. 69393 GROUP 2: 15 PPM FEMALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE __________________~~~~ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN OVARIES THYMUS ADRENAL GLANDS THYROIDS/ pARA FINAL BODY WT( G) ABS. (G) 1.81 4.33 1.25 0.97 0.59 0.41 0.0869 0.1759 0.0550 0.0164 166. REL. 1.090 2.608 0.753 0.584 0.355 0.247 0.052 0.106 0.033 GENERAL COMMENT GROSS: MECWANICAL TRAUMA DISTAL TAIL BROKEN GENERAL COMMENT GROSS: MECHANICAL TRAUMA DISTAL TAIL MISSING NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN COLON DUODENUM LAC GLAND, EXOR EYES/ OPTIC N. HARDERIAN GLANDS HEART JEJUNUM KIDNEYS LIVER LUNGS MAMMARY GLAND LARYNX LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES PANCREAS OVIDUCTS THYMUS PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SPINAL CORD SPLEEN STOMACH TRACHEA URINARY BLADDER UTERUS P P 0.010 CECUM ESOPHAGUS ILEUM AORTA NERVE, SCIATIC OVARIES RECTUM SKIN THYROID GLANDS VAGINA TABLE 31 (PRIMARY NECROPSY) INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 55 GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 56 SPONSOR: HAP TASK FORCE INDIVIDUAL MACROSCOPIC FINDINGS ANIMAL NO. 69396 GROUP 2: 15 PPM FEMALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE ____________________________________~~~ ORGAN WEIGHT ABS.( G) BRAIN 1.76 LIVER 4.88 KIDNEYS 1.19 LUNGS 0.98 HEART 0.63 SPLEEN 0.47 OVARIES 0.1049 THYMUS 0.1716 ADRENAL GLANDS 0.0601 THYROIDS/ PARA 0.0147 FINAL BODY WT( G) 188. REL. 0.936 2.596 0.633 0.521 0.335 0.250 0.056 0.091 0.032 0.008 PITUITARY GROSS: SMALL SKELETAL MUSCLE GROSS: DISCOLORATION, DARK RED SURROUNDING MECHANICAL TRAUMA GENERAL COMMENT GROSS: MECHANICAL TRAUMA FRACTURE OF TIBIA AND FIBULA, LEFT NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN COLON DUODENUM LAC GLAND, EXOR EYES/ OPTIC N. HARDERIAN GLANDS HEART JEJUNUM KIDNEYS LIVER LUNGS MAMMARY GLAND LARYNX LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES PANCREAS OVIDUCTS THYMUS SAL. GLAND MAND SKIN SPINAL CORD STOMACH THYROID GLANDS TRACHEA UTERUS VAGINA P P I?

CECUM ESOPHAGUS ILEUM AORTA NERVE, SCIATIC OVARIES RECTUM SPLEEN URINARY BLADDER GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROFSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 57 ANIMAL NO. 69409 GROUP 2: 15 PPM FEMALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE ______________________________________~~~ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN ABS.( G) 1.80 4.75 1.21 0.96 0.64 0.43 0.0912 0.1997 0.0576 0.0152 180. REL. 1.000 2.639 0.672 0.533 0.356 0.239 0.051 0.111 0.032 0.008 GENERAL COMMENT GROSS: MECHANICAL TRAUMA DISTAL TAIL BROKEN NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN COLON DUODENUM LAC GLAND, EXOR EYES/ OPTIC N. HARDERIAN GLANDS HEART JEJUNUM KIDNEYS LIVER LUNGS MAMMARY GLAND LARYNX LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES PANCREAS OVIDUCTS THYMUS P CECUM ESOPHAGUS ILEUM AORTA NERVE, SCIATIC OVARIES RECTUM SKIN THYROID GLANDS OVARIES THYMUS ADRENAL GLANDS THYROIDS/ PARA FINAL BODY WT( G) PITUITARY SPINAL CORD ;&, LAND MAND SKELETAL MUSCLE STOMACH TRACHEA URINARY BLADDER UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSORcHAP TASK FORCE ANIMAL NO. 69413 GROUP 2: 1 ________________ _______  ORGAN WEIGHT ABS.( G) BRAIN 1.87 LIVER 4.45 KIDNEYS 1.37 LUNGS 0.97 HEART 0.69 SPLEEN 0.47 OVARIES 0.1115 THYMUS 0.1989 ADRENAL GLANDS 0.0656 THyROIDS/ PARA 0.0120 FINAL BODY WT( G) 173. TABLE 31 (PRIMARY NE~ ROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 58 _   _   _  _ REL. 1.081 2.572 0.792 0.561 0.399 0.272 0.064 0.115 0.038 0.007 5 PPM FEMALE SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE _____________________________________~~~ NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM COLON DUODENUM LAC GLAND, EXOR ESOPHAGUS EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM JEJUNUM KIDNEYS LIVER AORTA LUNGS MAMMARY GLAND LARYNX NERVE. SCIATIC LYMPH NODE, TR/ BR LYMPH NODE,. MED NASAL TISSUES OVARIES PANCREAS OVIDUCTS THYMUS RECTUM PITUITARY SPINAL CORD TRACHEA SAL. GLAND MAND SKELETAL MUSCLE SKIN SPLEEN STOMACH THYROID GLANDS URINARY BLADDER UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 59 ANIMAL NO. 63435 GROUP ORGAN WEIGHT ABS. (G) BRAIN 1.72 LIVER 4.49 KIDNEYS 1.30 LUNGS 0.97 HEART 0.61 SPLEEN 0.37 OVARIES 0.0769 THYMUS 0.1705 ADRENAL GLANDS 0.0519 THYROIDS/ PARA 0.0146 FINAL BODY WT( G) 179. 2: 15 PPM FEMALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE REL. NO SIGNIFICANT 0.961 CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM 2.508 COLON DUODENUM LAC GLAND, EXOR ESOPHAGUS 0.726 EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM 0.542 JEJUNUM KIDNEYS LIVER AORTA 0.341 LUNGS MAMMARY GLAND LARYNX NERVE, SCIATIC 0.207 LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES OVARIES 0.043 PANCREAS OVIDUCTS THYMUS RECTUM 0.095 PITUITARY 0.029 SPINAL CORD 0.008 TRACHEA SAL. GLAND MAND SKELETAL MUSCLE SKIN SPLEEN STOMACH THYROID GLANDS URINARY BLADDER UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 60 ANIMAL NO. 69359 GROUP 3: 40 PPM FEMALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE _____________________________________~~ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN OVARIES THYMUS ADRENAL GLANDS THYROIDS/ PARA FINAL BODY WT( G) ABS.( G) REL. 1.74 1.030 4.26 2.521 1.21 0.716 0.83 0.491 0.61 0.361 0.44 0.260 0.0943 0.056 0.2077 0.123 0.0557 0.033 NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM COLON DUODENUM EYES/ OPTIC N. HARDERIAN GLANDS JEJUNUM KIDNEYS LUNGS MAMMARY GLAND LYMPH NODE, TR/ BR LYMPH NODE, MED PANCREAS OVIDUCTS PITUITARY SAL. GLAND MAND SPINAL CORD SPLEEN URINARY BLADDER 0.0165 0.010 TRACHEA 169. BRAIN CECUM LAC GLAND, EXOR ESOPHAGUS HEART ILEUM LIVER AORTA LARYNX NERVE, SCIATIC NASAL TISSUES OVARIES THYMUS RECTUM SKELETAL MUSCLE SKIN STOMACH THYROID GLANDS UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, p PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NEcROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 61 ANIMAL NO. 69369 GROUP 3:

________ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN OVARIES THYMUS ADRENAL GLANDS THYROIDS/ PARA FINAL BODY WT( G)  _ _ _ _ _ _ _ _ ABS.( G) 1.82 4.41 1.31 1.00 0.62 0.42 0.0831 0.1986 0.0579 0.0113 184. REL. 0.989 2.397 0.712 0.543 0.337 0.228 0.045 0.108 0.031 0.006 40 PPM FEMALE SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE ________________~~~~ NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM COLON DUODENUM LAC GLAND, EXOR ESOPHAGUS EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM JEJUNUM KIDNEYS LIVER AORTA LUNGS MAMMARY GLAND LARYNX NERVE, SCIATIC LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES OVARIES PANCREAS OVIDUCTS THYMUS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 62 ANIMAL NO. 69370 GROUP 3:

____ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN OVARIES THYMUS ADRENAL GLANDS THYRQIDS/ PARA FINAL BODY WT( G)  _ _  _ _ _ _ _ _  _ _ _ _ _ ABS. (G) REL. 1.69 0.904 4.95 2.647 1.20 0.642 0.97 0.519 0.64 0.342 0.46 0.0924 0.1842 0.0578 0.0157 187. 0.246 0.049 0.099 0.031 0.008 40 PPM FEMALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM COLON DUODENUM LAC GLAND. EXOR ESOPHAGUS EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM JEJUNUM KIDNEYS LIVER AORTA LUNGS MAMMARY GLAND LARYNX NERVE, SCIATIC LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES OVARIES PANCREAS OVIDUCTS THYMUS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 63 ANIMAL NO. 69380 GROUP 3: 40 PPM FEMALE SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE ______________________________________~~~~~~~~~~~~~ ORGAN WEIGHT ABS.( G) BRAIN 1.81 LIVER 4.77 KIDNEYS 1.34 LUNGS 0.97 HEART 0.66 SPLEEN 0.46 OVARIES 0.0870 THYMUS 0.2330 ADRENAL GLANDS 0.0545 THYROIDS/ PARA 0.0123 FINAL BODY NT( G) 202. REL. 0.896 2.361 0.663 0.480 0.327 0.228 0.043 0.115 0.027 0.006 NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS COLON EYES/ OPTIC N. JEJUNUM LUNGS LYMPH NODE, TR/ BR PANCREAS PITUITARY SPINAL CORD TRACHEA STERNUM BRAIN CECUM DUODENUM LAC GLAND, EXOR ESOPHAGUS HARDERIAN GLANDS HEART ILEUM KIDNEYS LIVER AORTA MAMMARY GLAND LARYNX NERVE, SCIATIC LYMPH NODE, MED NASAL TISSUES OVARIES OVIDUCTS THYMUS RECTUM SAL. GLAND MAND SKELETAL MUSCLE SKIN SPLEEN STOMACH THYROID GLANDS URINARY BLADDER UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 64 ANIMAL NO. 69388 GROUP 3: 40 PPM FEMALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE _____________________________________

ORGAN WEIGHT ABS.( G) 1.82 4.93 1.30 0.96 0.70 REL. NO SIGNIFICANT 1.040 CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN 2.817 COLON DUODENUM LAC GLAND, EXOR 0.743 EYES/ OPTIC N. HARDERIAN GLANDS HEART 0.549 JEJUNUM KIDNEYS LIVER 0.400 LUNGS MAMMARY GLAND LARYNX 0.280 LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES 0.069 PANCREAS OVIDUCTS THYMUS 0.113 PITUITARY SAL. GLAND MAND SKELETAL MUSCLE 0.036 SPINAL CORD SPLEEN STOMACH 0.009 TRACHEA URINARY BLADDER UTERUS BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN OVARIES THYMUS ADRENAL GLANDS THYROIDS/ PARA FINAL BODY WT( G) 0.49 0.1199 0.1980 0.0637 0.0154 175. CECUM ESOPHAGUS ILEUM AORTA NERVE, SCIATIC OVARIES RECTUM SKIN THYROID GLANDS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 65 ANIMAL NO. 69394 GROUP 3: 40 PPM FEMALE SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE ______________ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN OVARIES THYMUS ADRENAL GLANDS THYROIDS/ PARA FINAL BODY WT( G) ________ ABS.( G) 1.73 4.56 1.30 1.00 0.64 0.48 0.0891 0.2163 0.0488 0.0121 182.    _ _ _ _

REL ,

0.951 2.505 0.714 0.549 0.352 0.264 0.049 0.119 0.027 0.007 ______________________________________~~. NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM COLON DUODENUM EYES/ OPTIC N. HARDERIAN GLANDS JEJUNUM KIDNEYS LUNGS MAMMARY GLAND LYMPH NODE, TR/ BR LYMPH NODE, MED PANCREAS OVIDUCTS PITUITARY SAL. GLAND MAND SPINAL CORD SPLEEN TRACHEA URINARY BLADDER BRAIN CECUM LAC GLAND, EXOR ESOPHAGUS HEART ILEUM LIVER AORTA LARYNX NERVE, SCIATIC NASAL TISSUES OVARIES THYMUS RECTUM SKELETAL MUSCLE SKIN STOMACH THYROID GLANDS UTERUS VAGINA GROSS GRADE CODE: I SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE ANIMAL NO. 69426 GROUP ____________________ ORGAN WEIGHT ABS.( G) BRAIN 1.79 LIVER 4.98 KIDNEYS 1.40 LUNGS 0.93 HEART 0.67 SPLEEN 0.47 OVARIES 0.0974 THYMUS 0.1986 ADRENAL GLANDS 0.0553 THYROIDSjPARA 0.0128 FINAL BODY WT( G) 193. __ TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 66 3: 40 PPM FEMALE SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE ___________~~ REL. NO SIGNIFICANT 0.927 CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM 2.580 COLON DUODENUM LAC GLAND, EXOR ESOPHAGUS 0.725 EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM 0.482 JEJUNUM KIDNEYS LIVER AORTA 0.347 LUNGS MAMMARY GLAND LARYNX NERVE, SCIATIC 0.244 LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES OVARIES 0.050 PANCREAS OVIDUCTS THYMUS RECTUM 0.103 PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN 0.029 SPINAL CORD SPLEEN STOMACH THYROID GLANDS 0.007 TRACHEA URINARY BLADDER UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: UAP TASK FORCE ANIMAL NO. 69428 GROUP 3:

____________ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN OVARIES THYMUS ADRENAL GLANDS ABS. (G) 1.75 4.41 1.20 0.94 0.63 0.47 0.0808 0.1873 0.0495 TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 67 REL. 1.042 2.625 0.714 0.560 0.375 0.280 0.048 0.111 0.029 THYROIDS/ PARA 0.0108 0.006 FINAL BODY WT( G) 168. 40 PPM FEMALE SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE .___________________________________~~~~~ NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM COLON DUODENUM LAC GLAND, EXOR ESOPHAGUS EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM JEJUNUM KIDNEYS LIVER AORTA LUNGS MAMMARY GLAND LARYNX NERVE, SCIATIC LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES OVARIES PANCREAS OVIDUCTS THYMUS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 68 ANIMAL NO. 69433 GROUP 3: 40 PPM FEMALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE ________________________________________ ORGAN WEIGHT ABS. (G) BRAIN 1.79 LIVER 4.91 KIDNEYS 1.38 LUNGS 1.06 HEART 0.66 SPLEEN 0.46 OVARIES 0.1062 THYMUS 0.2110 ADRENAL GLANDS 0.0488 THYROIDS/ PARA 0.0167 FINAL BODY WT( G) 111. REL. 1.011 2.714 0.780 0.599 0.373 0.260 0.060 0.119 0.028 0.009 GENERAL COMMENT GROSS: ORGAN DAMAGED AT NECROPSY EYE, LEFT NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN COLON DUODENUM LAC GLAND, EXOR EYES/ OPTIC N. HARDERIAN GLANDS HEART JEJUNUM KIDNEYS LIVER LUNGS MAMMARY GLAND LARYNX LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES PANCREAS OVIDUCTS THYMUS PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SPINAL CORD SPLEEN STOMACH TRACHEA URINARY BLADDER UTERUS P CECUM ESOPHAGUS ILEUM AORTA NERVE, SCIATIC OVARIES RECTUM SKIN THYROID GLANDS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT TABLE 31 (PRIMARY NECRO~ SY) PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 69 SPONS~ R: HAP TASK FORCE INDIVIDUAL MACROSCOPIC FINDINGS ANIMAL NO. 69436 GROUP 3: 40 PPM FEMALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE _________________________________~~~~~ ORGAN WEIGHT ABS. (G) BRAIN 1.78 REL. NO SIGNIFICANT 0.927 CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN 2.615 COLON DUODENUM LAC GLAND, EXOR 0.724 EYES/ OPTIC N. HARDERIAN GLANDS HEART 0.495 JEJUNUM KIDNEYS LIVER CECUM ESOPHAGUS ILEUM AORTA NERVE, SCIATIC OVARIES RECTUM SKIN THYROID GLANDS VAGINA LIVER KIDNEYS LUNGS HEART SPLEEN OVARIES THYMUS ADRENAL GLANDS THYROIDS/ PARA FINAL BODY WT( G) 5.02 1.39 0.95 0.62 0.323 0.43 0.224 0.0750 0.2076 0.0517 0.0173 192. 0.039 0.108 0.027 0.009 LUNGS MAMMARY GLAND LARYNX LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES PANCREAS OVIDUCTS THYMUS PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SPINAL CORD SPLEEN STOMACH TRACHEA URINARY BLADDER UTERUS GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 70 ANIMAL NO. 69375 GROUP 4: 100 PPM FEMALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE ________________________________________ ORGAN WEIGHT BRAIN ABS.( G) 1.74 4.70 1.25 0.89 0.60 0.42 0.0816 0.2034 0.0505 REL. 1.018 2.749 0.731 0.520 0.351 0.246 SKIN GROSS: MATTING, YELLOW P UROGENITAL NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM COLON DUODENUM LAC GLAND, EXOR ESOPHAGUS EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM LIVER KIDNEYS LUNGS HEART SPLEEN OVARIES THYMUS ADRENAL GLANDS THYROIDS/ PARA FINAL BODY WT( G) 0.0160 171. 0.048 0.119 0.030 0.009 JEJUNUM KIDNEYS LIVER AORTA LUNGS MAMMARY GLAND LARYNX NERVE, SCIATIC LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES OVARIES PANCREAS OVIDUCTS THYMUS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 71 ANIMAL NO. 69379 GROUP _________________________ ORGAN WEIGHT ABS. (G) BRAIN 1.79 LIVER 4.98 KIDNEYS 1.37 LUNGS 1.11 HEART 0.65 SPLEEN 0.46 OVARIES 0.1223 THYMUS 0.2146 ADRENAL GLANDS 0.0633 THYROIDS/ PARA 0.0158 FINAL BODY WT( G) 181. 4: 100 PPM FEMALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE ________________________________________ REL. LIVER GROSS: AREA( S), RAISED P 0.989 ONE, 3 X 3 X 1 MM, WHITE, MEDIAN LOBE 2.751 LUNGS GROSS: AREA( S), WHITE P 0.757 FEW, IRREGULARLY SHAPED, ALL LOBES 0.613 NO SIGNIFICANT 0.359 CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM 0.254 COLON DUODENUM LAC GLAND, EXOR ESOPHAGUS 0.068 EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM 0.119 0.035 0.009 JEJUNUM KIDNEYS AORTA MAMMARY GLAND LARYNX NERVE, SCIATIC LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES OVARIES PANCREAS OVIDUCTS THYMUS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT TABLE 31 (PRIMARY NECROPSY) PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE SPONSOR: HAP TASK FORCE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 72 ANIMAL NO. 69383 GROUP . _______ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN OVARIES THYMUS ADRENAL GLANDS THYROlDS/ PARA FINAL BODY WT( G) _____ __ ABS.( G) 1.79 4.88 1.28 1.03 0.67 0.46 0.0952 0.1797 0.0572 0.0136 178. 4:

__ REL. 1.006 2.742 0.719 0.579 0.376 0.258 0.053 0.101 0.032 0.008 .OO PPM FEMALE SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE ___________________ OVARIES GROSS: AREA( S), WHITE P MULTIPLE, PINPOINT, BILATERAL NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM COLON DUODENUM LAC GLAND, EXOR ESOPHAGUS EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM JEJUNUM KIDNEYS LIVER AORTA LUNGS MAMMARY GLAND LARYNX NERVE, SCIATIC LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES PANCREAS OVIDUCTS THYMUS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE ANIMAL NO. 69385 GROUP ________ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN OVARIES 0.0951 THYMUS 0.2063 ADRENAL GLANDS 0.0547 THyROIDS/ PARA 0.0117 FINAL BODY WT( G) 187. ABS. (G) 1.73 4.91 1.47 0.89 0.73 0.45 TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 73 4: 100 PPM FEMALE SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE ____________________________________ REL. NO SIGNIFICANT 0.925 CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM 2.626 COLON DUODENUM LAC GLAND, EXOR ESOPHAGUS 0.786 EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM 0.476 JEJUNUM KIDNEYS LIVER AORTA 0.390 LUNGS MAMMARY GLAND LARYNX NERVE. SCIATIC 0.241 LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES OVARIk 0.051 PANCREAS OVIDUCTS THYMUS RECTUM 0.110 PITUITARY 0.029 SPINAL CORD 0.006 TRACHEA SAL. GLAND MAND SKELETAL MUSCLE SKIN SPLEEN STOMACH THYROID GLANDS URINARY BLADDER UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 74 ANIMAL NO. 69392 GROUP ________  ___ __. ORGAN WEIGHT ABS.( G) BRAIN 1.74 LIVER 4.43 KIDNEYS 1.30 LUNGS 0.88 HEART 0.60 SPLEEN 0.39 OVARIES 0.0763 THYMUS 0.1873 ADRENAL GLANDS 0.0508 THYROIDS/ PARA 0.0104 4: 100 PPM FEMALE SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE ____________________________________~~~~~

REL. NO SIGNIFICANT 1.055 CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM 2.685 COLON DUODENUM LAC GLAND, EXOR ESOPHAGUS 0.788 EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM 0.533 JEJUNUM KIDNEYS LIVER AORTA 0.364 LUNGS MAMMARY GLAND LARYNX NERVE. SCIATIf! 0.236 LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES OVARIES 0.046 PANCREAS OVIDUCTS THYMUS RECTUM 0.114 0.031 0.006 PITUITARY SPINAL CORD TRACHEA SAL. GLAND MAND SKELETAL MUSCLE SKIN SPLEEN STOMACH THYROID GLANDS URINARY BLADDER UTERUS VAGINA 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 75 ANIMAL NO. 69402 GROUP ____________ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN OVARIES THYMUS ADRENAL GLANDS THYROIDS/ PARA FINAL BODY WT( G) ABS. (G) 1.72 5.30 1.38 0.96 0.66 0.45 0.0933 0.2100 0.0541 0.0099 186. 4: 100 PPM FEMALE SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE ____________~~~ REL. NO SIGNIFICANT 0.925 CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM 2.849 COLON DUODENUM LAC GLAND, EXOR ESOPHAGUS 0.742 EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM 0.516 JEJUNUM KIDNEYS LIVER AORTA 0.355 LUNGS MAMMARY GLAND LARYNX NERVE, SCIATIC 0.242 LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES OVARIES 0.050 PANCREAS OVIDUCTS THYMUS RECTUM 0.113 PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN 0.029 SPINAL CORD SPLEEN STOMACH THYROID GLANDS 0.005 TRACHEA URINARY BLADDER UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 76 ANIMAL NO. 69408 GROUP 4: 100 PPM FEMALE SCHEDULED EUTH 08/ 31/ 01 DATE OF DEATH: 08/ 31/ 01 STUDY DAY: 92 GRADE _____________________________________~~~~ ORGAN WEIGHT ABS. (G) REL. GENERAL COMMENT GROSS: ORGAN DAMAGED AT NECROPSY P BRAIN 1.80 0.994 PITUITARY LIVER 4.87 2.691 NO SIGNIFICANT KIDNEYS 1.40 0 LUNGS 1.00 0 HEART 0.67 0 SPLEEN 0.46 0 OVARIES 0.0958 0 THYMUS 0.1681 0 ADRENAL GLANDS 0.0548 0 THYROIDS/ PARA 0.0106 0 FINAL BODY WT( G) 181. 773 CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM 552 COLON DUODENUM LAC GLAND, EXOR ESOPHAGUS 370 254 053 093 030 006 EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM JEJUNUM KIDNEYS LIVER AORTA LUNGS MAMMARY GLAND LARYNX NERVE, SCIATIC LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES OVARIES PANCREAS OVIDUCTS THYMUS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 77 ANIMAL NO. 69415 GROUP 4: 100 PPM FEMALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE ORGAN WEIGHT ABS.( G) REL. BRAIN 1.80 0.984 LIVER 4.88 2.667 KIDNEYS 1.29 0.705 LUNGS 0.97 0.530 HEART 0.76 0.415 SKIN GROSS: MATTING, YELLOW UROGENITAL NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM COLON DUODENUM EYES/ OPTIC N. HARDERIAN GLANDS JEJUNUM KIDNEYS LUNGS MAMMARY GLAND LYMPH NODE, TR/ BR LYMPH NODE, MED PANCREAS OVIDUCTS PITUITARY SAL. GLAND MAND SPLEEN STOMACH URINARY BLADDER UTERUS P CECUM BRAIN LAC GLAND, EXOR HEART LIVER LARYNX NASAL TISSUES THYMUS SKELETAL MUSCLE THYROID GLANDS VAGINA ESOPHAGUS ILEUM AORTA NERVE, SCIATIC OVARIES RECTUM SPINAL CORD TRACHEA SPLEEN OVARIES THYMUS ADRENAL GLANDS THYROIDS/ PARA FINAL BODY WT( G) 0.45 0.246 0.1102 0.060 0.2245 0.0537 0.0182 183. 0.123 0.029 0.010 GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE ANIMAL NO. 69427 GROUP ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN OVARIES THYMUS ADRENAL GLANDS THYROIDS/ PAR?+ FINAL BODY WT( G) .___  ____ ABS. (G) 1.75 4.89 1.31 1.10 0.66 0.47 0.1062 0.2529 0.0619 0.0198 178. TABLE 31 (PRIMARY NECR~ PSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 78 4: 100 PPM FEMALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE __________________________________

REL. SKIN GROSS: MATTING, YELLOW P 0.983 UROGENITAL; ANOGENITAL 2.747 NO SIGNIFICANT 0.736 CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM 0.618 COLON DUODENUM LAC GLAND, EXOR ESOPHAGUS 0.371 EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM 0.264 JEJUNUM KIDNEYS LIVER AORTA 0.060 LUNGS MAMMARY GLAND LARYNX NERVE, SCIATIC 0.142 LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES OVARIES 0.035 PANCREAS OVIDUCTS THYMUS RECTUM 0.011 PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER UTERUS VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 31 (PRIMARY NECROPSY) 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL MACROSCOPIC FINDINGS PAGE 79 ANIMAL NO. 69431 GROUP 4: 100 PPM FEMALE SCHEDULED EUTH 08/ 30/ 01 DATE OF DEATH: 08/ 30/ 01 STUDY DAY: 91 GRADE _____________________________________ ORGAN WEIGHT BRAIN LIVER KIDNEYS LUNGS HEART SPLEEN OVARIES THYMUS ADRENAL GLANDS THYROIDS/ PARA FINAL BODY WT( G) ABS.( G) 1.78 4.91 1.47 1.04 0.72 REL. 0.983 2.713 0.812 0.575 0.398 UTERUS GROSS: CONTENTS, CLEAR FLUID P BOTH HORNS NO SIGNIFICANT CHANGES OBSERVED GROSS: ADRENAL GLANDS STERNUM BRAIN CECUM 0.49 0.271 0.1014 0.056 COLON DUODENUM LAC GLAND, EXOR ESOPHAGUS EYES/ OPTIC N. HARDERIAN GLANDS HEART ILEUM 0.2008 0.0645 0.0162 181. 0.111 0.036 0.009 JEJUNUM KIDNEYS LIVER AORTA LUNGS MAMMARY GLAND LARYNX NERVE, SCIATIC LYMPH NODE, TR/ BR LYMPH NODE, MED NASAL TISSUES OVARIES PANCREAS OVIDUCTS THYMUS RECTUM PITUITARY SAL. GLAND MAND SKELETAL MUSCLE SKIN SPINAL CORD SPLEEN STOMACH THYROID GLANDS TRACHEA URINARY BLADDER VAGINA GROSS GRADE CODE: l SLIGHT, 2 MODERATE, 3 MARKED, P PRESENT PGRHv4.28 01/ 18/ 2002 FINAL REPORT Volume 2 of 2 (Individual Tables 32  34 and Appendices)

STUDY TITLE A 90 Day Inhalation Toxicity Study of 1, 1,2 Trichloroethane (1, 1,2 TCE) in Rats (With Satellite Groups for Pharrnacokinetic Evaluations in Rats and Mice)

STUDY NUMBER WIL 4 17002 DATA REQUIREMENT U. S. EPA TSCA 799.9346 STUDY DIRECTOR Daniel T. Kirkpatrick, Ph. D., D. A. B. T.

STUDY INITIATION DATE May 15,200l STUDY COMPLETION DATE May 23,2002 PERFORMING LABORATORY WIL Research Laboratories, Inc. 1407 George Road Ashland, OH 44805 928 1 SPONSOR HAP Task Force P. O. Box 331 Millwood, VA 22646 303 of 619 WIL 4 17002 HAP Task Force 1, 1,2 Trichloroethane TABLES 32  34 304of619 TABLE 32 PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 1 SPONSOR: HAP TASK FORCE INDIVIDUAL ORGAN WEIGHTS AND FINAL BODY WEIGHTS [Gl WEEK 13 MALE GROUP: 0 PPM ______________________________________

ANIMAL _ _ _ _ _ _ _ _ _ 69302 69306 69308 69310 69312 69314 69317 69322 69337 69343 FBW( G) .____________________ 305. 291. 282. 264. 316. 281. 309. 280. 284. 263. BRAIN LIVER KIDNEYS LUNGS HEART 1.83 1.81 1.88 1.73 1.88 1.74 1.84 1.85 1.84 1.83 MEAN 288. 1.82 S. D. 17.9 0.051 N 10 10 FBW = FINAL BODY WEIGHT ___________________ __________ 8.57 1.89 8.25 2.09 8.40 1.97 7.35 1.87 8.46 2.16 7.49 1.87 8.37 2.02 7.44 2.00 8.03 1.92 7.80 1.80 1.25 1.15 1.10 1.15 1.25 1.19 1.21 1.26 1.18 1.18 _ 0.99 0.90 0.98 0.95 1.01 0.85 0.89 0.87 1.02 0.89 8.02 1.96 1.19 0.94 0.463 0.111 0.052 0.062 10 10 10 10 TABLE 32 PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 2 SPONSOR: HAP TASK FORCE INDIVIDUAL ORGAN WEIGHTS AND FINAL BODY WEIGHTS [G] WEEK 13 MALE GROUP: 15 PPM _______________________________~

ANIMAL ____ ___

69305 69316 69320 69323 69326 69335 69336 69338 69345 69347 FBW( G) _____________

261. 1.87 307. 1.92 317. 1.87 323. 1.84 334. 1.98 320. 1.95 300. 1.86 273. 1.87 307. 2.01 282. 1.76 BRAIN LIVER KIDNEYS ____________________________________~

7.12 1.87 9.78 2.25 8.75 2.20 8.85 2.11 8.99 2.19 8.86 2.24 8.71 2.08 7.22 1.81 8.66 2.29 8.02 1.99 LUNGS _________________ 0.96 1.34 1.40 1.31 1.40 0.98 1.26 1.08 1.21 1.11 1.27 1.23 HEART .__ 0.81 1.00 1.00 0.99 0.99 0.88 0.98 0.87 MEAN 302. 1.89 8.50 2.10 1.25 0.96 S. D. 23.5 0.073 0.819 0.165 0.135 0.080 N 10 10 10 10 10 10 2 FBW = FINAL BODY WEIGHT a TABLE 32 PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 3 SPONSOR: HAP TASK FORCE INDIVIDUAL ORGAN WEIGHTS AND FINAL BODY WEIGHTS [G] WEEK 13 MALE GROUP: 40 PPM ______________________________________

ANIMAL ______. 69303 69309 69313 69315 69324 69328 69339 69344 69348 69350 FBW( G) ________________. 255. 308. 310. 253. 333. 322. 270. 316. 292. 280.

MEAN 294. S. D. 28.3 N 10 FBW = FINAL BODY WEIGHT BRAIN LIVER .__ ____ _________________________

1.61 6.40 1.89 8.02 1.91 9.01 1.85 6.64 1.95 8.96 1.90 8.59 1.77 7.44 1.84 9.00 1.92 8.36 1.88 7.28 1.85 7.97 2.10 1.17 0.96 0.099 0.980 0.240 0.149 0.064 10 10 10 10 10 KIDNEYS LUNGS ___________ ______________________________ 1.76 0.99 2.44 1.39 2.26 1.22 1.74 1.05 2.24 1.25 2.20 1.30 1.94 0.90 2.32 1.24 2.17 1.19 1.95 1.20 HEART ____ 0.89 0.97 1.03 0.87 1.01 1.06 0.91 1.01 0.95 0.92 TABLE 32 PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 4 SPONSOR: HAP TASK FORCE INDIVIDUAL ORGAN WEIGHTS AND FINAL BODY WEIGHTS [Gl WEEK 13 MALE GROUP: 100 PPM _____________________________________

ANIMAL FBW( G) _______ _______________

69304 323. 69307 327. 69318 308. 69319 305. 69327 303. 69329 293. 69332 293. 69334 288. 69342 306. 69346 303. BRAIN __________________

1.91 1.95 1.83 1.87 1.84 1.87 1.90 1.82 1.79 1.78 LIVER KIDNEYS ________________ .__________^__ 8.98 2.36 9.49 2.22 8.70 2.15 8.46 2.19 7.77 2.01 8.34 2.21 7.56 1.94 8.48 2.17 8.46 2.22 8.89 2.24 LUNGS HEART 1.30 1.36 1.21 1.27 1.22 1.07 1.13 0.99 0.94 0.92 0.95 0.97 0.97 0.88 NA 1.21 1.11 1.19 1.14 1.19 MEAN 305. 1.86 8.51 2.1.7 1.22 S. D. 12.5 0.054 0.562 0.119 0.074 N 10 10 10 10 10 w E FBW = FINAL BODY WEIGHT NA = NOT APPLICABLE % (3 ;; 0.98 0.077 9 TABLE 32 PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 5 SPONSOR: HAP TASK FORCE INDIVIDUAL ORGAN WEIGHTS AND FINAL BODY WEIGHTS [Gl WEEK 13 MALE GROUP: 0 PPM __________________~~ EPIDID ADRENAL THYROIDS ANIMAL SPLEEN YMIDES TESTES THYMUS GLANDS /PARA __________________~ 69302 0.55 0.99 2.87 0.1814 0.0451 0.0197 69306 0.53 0.98 3.16 0.1619 0.0595 0.0212 69308 0.58 1.00 3.03 0.1835 0.0627 0.0208 69310 0.54 0.99 2.79 0.1835 0.0546 0.0112 69312 0.66 1.04 3.43 0.2075 0.0638 0.0148 69314 0.57 0.90 2.98 0.1740 0.0487 0.0151 69317 0.59 0.91 3.13 0.2070 0.0393 0.0105 69322 0.50 0.94 2.99 0.1670 0.0602 0.0146 69337 0.55 1.07 3.10 0.1882 0.0592 0.0228 69343 0.61 0.91 2.83 0.1493 0.0518 0.0192 MEAN 0.57 0.97 3.03 0.1803 0.0545 0.0170 S. D. 0.045 0.057 0.188 0.01842 0.00812 0.00432 N 10 10 10 10 10 10 PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 32 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL ORGAN WEIGHTS AND FINAL BODY WEIGHTS [G]

SPLEEN _______________

0.49 0.70 0.62 0.60 0.67 0.67 0.60 0.56 0.65 0.59 ANIMAL ________________

69305 69316 69320 69323 69326 69335 69336 69338 69345 69347 MALE GROUP: 15 PPM ____________________________________~~ EPIDID ADRENAL THYROIDS YMIDES TESTES THYMUS GLANDS /PARA ____________

0.96 3.04 0.1574 0.0607 0.0213 0.99 3.39 0.2055 0.0629 0.0208 0,95 3.08 0.2047 0.0537 0.0170 0.96 3.11 0.1898 0.0577 0.0178 1.09 3.39 0.2244 0.0521 0.0249 1.03 3.24 0.1967 0.0541 0.0145 0.94 2.90 0.2001 0.0618 0.0146 0.94 3.13 0.1938 0.0564 0.0146 1.08 3.20 0.2254 0.0587 0.0139 0.88 2.73 0.2210 0.0519 0.0253 MEAN 0.62 0.98 3.12 0.2019 0.0570 0.0185 S. D. 0.062 0.066 0.204 0.02018 0.00401 0.00435 N 10 10 10 10 10 10 PAGE 6 WEEK 13 PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 32 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL ORGAN WEIGHTS AND FINAL BODY WEIGHTS [G] PAGE I WEEK 13 MALE GROUP: ______________________________________

ANIMAL SPLEEN EPIDID YMIDES ___________ 69303 69309 69313 69315 69324 69328 69339 69344 69348 69350 MEAN 0.59 0.96 3.11 0.1919 0.0557 0.0165 S. D. 0.091 0.090 0.353 0.03725 0.00581 0.00296 N 10 10 10 10 10 10 0 0 41 59 64 42 70 63 51 68 60 61 _______________ 0.80 1.01 0.96 0.94 1.08 1.08 0.88 0.99 0.99 0.87 40 PPM _____________~~ ADRENAL THYROIDS TESTES THYMUS GLANDS /PARA 2.68 0.0972 0.0495 0.0144 3.25 0.2094 0.0654 0.0151 3.13 0.2268 0.0598 0.0162 2.81 0.1794 0.0474 0.0188 3.22 0.2108 0.0601 0.0163 3.95 0.2050 0.0478 0.0163 2.80 0.1799 0.0572 0.0165 3.11 0.2125 0.0562 0.0148 3.16 0.1814 0.0565 0.0236 3.00 0.2170 0.0566 0.0128 TABLE 32 PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 8 SPQNSOR: HAP TASK FORCE INDIVIDUAL ORGAN WEIGHTS AND FINAL BODY WEIGHTS [G] WEEK 13 MALE GROUP: 100 PPM ___________________________________ EPIDID ADRENAL THYROIDS ANIMAL SPLEEN YMIDES TESTES THYMUS GLANDS /PARA ________________________________________ 69304 0.76 1.09 3.50 0.2438 0.0674 0.0140 69307 0.65 1.06 3.31 0.2473 0.0637 0.0197 69318 0.64 0.95 3.09 0.2204 0.0538 0.0228 69319 0.66 1.01 3.30 0 :1970 0 . 050.8 0.0152 69327 0.64 0.98 3.08 0.1762 0.0472 0.0166 69329 0.62 1.01 2.98 0.1926 0.0536 0.0224 69332 0.56 1.11 3.05 0.1944 0.0499 0.0150 69334 0.60 0.97 3.06 0.1969 0.0572 0.0225 69342 0.55 0.96 3.01 0.1683 0.0562 0.0168 69346 0.57 0.97 3.00 0.1742 0.0533 0.0159 MEAN 0.63 1.01 3.14 0.2011 0.0553 0.0181 S. D. 0.062 0.057 0.172 0.02769 0.00620 0.00343 N 10 10 10 10 10 10 PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE ANIMAL FBW( G)     _   _ _ __________ 69354 196. 69366 179. 69367 196. 69378 180. 69384 168. 69398 197. 69404 199. 69416 192. 69419 193. 69432 179. BRAIN LIVER ____ 1.90 5.34 1.71 4.41 1.79 4.64 1.81 4.86 1.72 4.70 1.86 4.72 1.81 4.81 1.84 5.16 1.76 4.90 1.81 4.93 MEAN 188. 1.80 S. D. 10.5 0.059 N 10 10 w t; FBW = FINAL BODY WEIGHT % o\

5, ____ TABLE 32 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL ORGAN WEIGHTS AND FINAL BODY WEIGHTS [Gl FEMALE GROUP: 0 PPM HEART SPLEEN KIDNEYS LUNGS ______________________

1.35 0.97 1.27 0.93 1.36 0.96 1.27 1.03 1.14 0.95 1.32 0.91 1.40 0.99 1.40 0.98 1.31 0.96 1.34 0.94 THYMUS 0.65 0.49 0.59 0.44 0.62 0.52 0.65 0.44 0.60 0.44 0.68 0.48 0.68 0.50 0.67 0.50 0.70 0.48 0.68 0.51 OVARIES  _ _ _ _ _ _ _ _ 0.0923 0.0885 0.0744 0.0969 0.1006 0.1011 0.1018 0.1029 0.1059 0.0924 0.2110 0.1814 0.2682 0.2174 0.1931 0.2156 0.2587 0.2011 0.2328 0.1837 ADRENAL GLANDS .___ ___ 0.0645 0.0493 0.0457 0.0449 0.0491 0.0639 0.0567 0.0554 0.0506 0.0562 ________ THYROIDS /PARA ___________ 0.0128 0.0131 0.0216 0.0109 0.0163 0.0155 0.0127 0.0181 0.0161 0.0132 4.85 1.32 0.96 0.65 0.48 0.0957 0.2163 0.0536 0.0150 0.263 0.077 0.034 0.037 0.030 0.00928 0.02952 0.00691 0.00316 10 10 10 10 10 10 10 10 10 PAGE 9 WEEK 13 TABLE 32 PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 10 SPONSOR: HAP TASK FORCE INDIVIDUAL ORGAN WEIGHTS AND FINAL BODY WEIGHTS [G] WEEK 13 FEMALE GROUP: 15 PPM ______________________________________

ANIMAL FBW( G)  _  _ _    _   _ _ _ _ _   _  _ 69362 176. 69376 159. 69382 195. 69390 182. 69393 166. 63396 188. 69409 180. 69413 173. 69435 179. BRAIN ________ 1.66 1.71 1.79 1.74 1.81 1.76 1.80 1.87 1.72 LIVER __ ____ 4.79 4.49 4.64 4.42 4.33 4.88 4.75 4.45 4.49 KIDNEYS 1.36 1.15 1.27 1.37 1.25 1.19 1.21 1.37 1.30 MEAN 178. 1.76 4.58 1.27 0.97 0.64 0.45 0.0989 0.1910 0.0589 0.0135 S. D. 10.9 0.063 0.190 0.082 0.081 0.032 0.050 0.01719 0.02205 0.00529 0.00187 N 9 9 9 9 9 9 9 9 9 9 9 w FBW = FINAL BODY WEIGHT x 0 2 G __ LUNGS HEART SPLEEN OVARIES _ _ _ _ _ _ _ _ _ _ _ _    _  . _ _   _  . _ _ _   _ _  _ 1.10 0.66 0.53 0.1351 0.80 0.60 0.42 0.0985 0.93 0.65 0.46 0.0997 1.04 0.65 0.51 0.0858 0.97 0.59 0.41 0.0869 0.98 0.63 0.47 0.1049 0.96 0.64 0.43 0.0912 0.97 0.69 0.47 0.1115 0.97 0.61 0.37 0.0769 THYMUS 0.2288 0.1878 0.1678 0.2184 0.1759 0.1716 0.1997 0.1989 0.1705 ADRENAL THYROIDS GLANDS /PARA     _   _ _ _______ 0.0687 0.0103 0.0558 0.0128 0.0590 0.0129 0.0564 0.0126 0.0550 0.0164 0.0601 0.0147 0.0576 0.0152 0.0656 0.0120 0.0519 0.0146 PROJECT NO.: WlL 417002 SPONSOR: HAP TASK FORCE ANIMAL FBW( G) BRAIN _____________________ 69359 169. 1.74 69369 184. 1.82 69370 187. 1.69 69380 202. 1.81 69388 175. 1.82 69394 182. 1.73 69426 193. 1.79 69428 168. 1.75 69433 177. 1.79 69436 192. 1.78 MEAN 183. 1.77 S. D. 11.0 0.043 N 10 10 w t; FBW = FINAL BODY WEIGHT R cn G ____ TABLE 32 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL ORGAN WEIGHTS AND FINAL BODY WEIGHTS [G]

FEMALE GROUP: 40 PPM __ LIVER _____

4.26 4.41 4.95 4.77 4.93 4.56 4.98 4.41 4.91 5.02 LUNGS HEART KIDNEYS ____ 1.21 1.31 1.20 1.34 1.30 1.30 1.40 1.20 1.38 1.39 0.83 0.61 1.00 0.62 0.97 0.64 0.97 0.66 0.96 0.70 1.00 0.64 0.93 0.67 0.94 0.63 1.06 0.66 0.95 0.62 SPLEEN _____

0.44 0.42 0.46 0.46 0.49 0.48 0.47 0.47 0.46 0.43 _. OVARIES ___ 0.0943 0.0831 0.0924 0.0870 0.1199 0.0891 0.0974 0.0808 0.1062 0.0750 ____ THYMUS _  _ _ _ _ _ _

0.2077 0.1986 0.1842 0.2330 0.1980 0.2163 0.1986 0.1873 0.2110 0.2076 ADRENAL THYROIDS GLANDS /PARA 0.0557 0.0165 0.0579 0.0113 0.0578 0.0157 0.0545 0.0123 0.0637 0.0154 0.0488 0.0121 0.0553 0.0128 0.0495 0.0108 0.0488 0.0167 0.0517 0.0173 4.72 1.30 0.96 0.65 0.46 0.0925 0.2042 0.0544 0.0141 0.283 0.078 0.059 0.028 0.022 0.01308 0.01427 0.00479 0.00246 10 10 10 10 10 10 10 10 10 PAGE 11 WEEK 13 PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 32 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL ORGAN WEIGHTS AND FINAL BODY WEIGHTS [G]

FEMALE GROUP: 100 PPM PAGE 12 WEEK 13 ANIMAL ____ 69375 69379 69383 69385 69392 69402 69408 69415 69427 69431 FBW( G)  _ _ _  _ _ _ _   171. 181. 178. 187. 165. 186. 181. 183. 178. 181. BRAIN 1.74 1.79 1.79 1.73 1.74 1.72 1.80 1.80 1.75 1.78 MEAN 179. 1.76 S. D. 6.7 0.031 N 10 10 w s FBW = FINAL BODY WEIGHT % a 5 LIVER __ _____. 4.70 4.98 4.88 4.91 4.43 5.30 4.87 4.88 4.89 4.91 KIDNEYS LUNGS HEART ._____ 0.60 0.65 0.67 0.73 0.60 0.66 0.67 0.76 0.66 0.72 SPLEEN __ OVARIES  _   _  _ 0.0816 0.1223 0.0952 0.0951 0.0763 0.0933 0.0958 0.1102 0.1062 0.1014 THYMUS 1.25 0.89 1.37 1.11 1.28 1.03 1.47 0.89 1.30 0.88 1.38 0.96 1.40 1.00 1.29 0.97 1.31 1.10 1.47 1.04 _ _ _ _ _ _ 0.42 0.46 0.46 0.45 0.39 0.45 0.46 0.45 0.47 0.49 0.2034 0.2146 0.1797 0.2063 0.1873 0.2100 0.1681 0.2245 0.2529 0.2008 ADRENAL THYROIDS GLANDS /PARA _ _ _ _    _ ___________

0.0505 0.0160 0.0633 0.0158 0.0572 0.0136 0.0547 0.0117 0.0508 0.0104 0.0541 0.0099 0.0548 0.0106 0.0537 0.0182 0.0619 0.0198 0.0645 0.0162 4.88 1.35 0.99 0.67 0.45 0.0977 0.2048 0.0566 0.0142 0.217 0.078 0.084 0.052 0.027 0.01334 0.02387 0.00503 0.00349 10 10 10 10 10 10 10 I. 0 10 POFBWv4.1 01/ 18/ 2002 R: 01/ 18/ 2002 TABLE 33 PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 1 SPONSOR: HAP TASK FORCE INDIVIDUAL ORGAN WTS. RELATIVE TO FINAL BODY WTS. [G/ 100 G] WEEK 13 MALE GROUP: 0 PPM __________________~

ANIMAL FBW( G) BRAIN LIVER KIDNEYS LUNGS HEART ___________________

69302 305. 0.600 2.810 0.620 0.410 0.325 69306 291. 0.622 2.835 0.718 0.395 0.309 69308 282. 0.667 2.979 0.699 0.390 0.348 69310 264. 0.655 2.784 0.708 0.436 0.360 69312 316. 0.595 2.677 0.684 0.396 0.320 69314 281. 0.619 2.665 0.665 0.423 0.302 69317 309. 0.595 2.709 0.654 0.392 0.288 69322 280. 0.661 2.657 0.714 0.450 0.311 69337 284. 0.648 2.827 0.676 0.415 0.359 69343 263. 0.696 2.966 0.684 0.449 0.338 MEAN 288. 0.636 2.791 0.682 0.416 0.326 S. D. 17.9 0.0347 0.1170 0.0303 0.0231 0.0246 N 10 10 10 10 10 10 FBW = FINAL BODY WEIGHT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 33 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL ORGAN WTS. RELATIVE TO FINAL BODY WTS. [G/ 100 Gl MALE GROUP: 15 PPM PAGE 2 WEEK 13 ANIMAL FBW( G) _ _ _ _ _ _ _ _ _ ________________ 69305 261. 69316 307. 69320 317. 69323 323. 69326 334. 69335 320. 69336 300. 69338 273. 69345 307. 69347 282. BRAIN __________________ 0.716 0.625 0.590 0.570 0.593 0.609 0.620 0.685 0.655 0.624 LIVER KIDNEYS 2.728 0.716 3.186 0.733 2.760 0.694 2.740 0.653 2.692 0.656 2.769 0.700 2.903 0.693 2.645 0.663 2.821 0.746 2.844 0.706 MEAN 302. 0.629 2.809 S. D. 23.5 0.0450 0.1521 N 10 10 10 FBW = FINAL BODY WEIGHT LUNGS ________ 0.368 0.436 0.442 0.406 0.419 0.394 0.403 0.407 0.414 0.436 0.696 0.413 0.0315 0.0224 10 10 _______ HEART 0.310 0.326 0.315 0.307 0.293 0.338 0.330 0.322 0.319 0.309 0.317 0.0130 10 PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 33 90 DAY INHAL. STUDY OF l, l., 2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL ORGAN WTS. RELATIVE TO FINAL BODY WTS. [G/ 100 G] PAGE 3 WEEK 13 MALE GROUP: 40 PPM ____________________~~~~~~~~~~~

ANIMAL  __ _ 69303 69309 69313 69315 69324 69328 69339 69344 69348 69350 FBW( G) __________ 255. 308. 310. 253. 333. 322. 270. 316. 292. 280. BRAIN LIVER _______________________ 0.631 2.510 0.614 2.604 0.616 2.906 0,731 2.625 0.586 2.691 0.590 2.668 0.656 2.756 0.582 2.848 0.658 2.863 0.671 2.600 KIDNEYS _________________ 0.690 0.792 0.729 0.688 0.673 0.683 0.719 0.734 0.743 0.696 LUNGS ___________ 0.388 0.451 0.394 0.415 0.375 0.404 0.333 0.392 0.408 0.429 HEART .._____ 0.349 0.315 0.332 0.344 0.303 0.329 0.337 0.320 0.325 0.329 MEAN 294. 0.634 S. D. 28.3 2.707 0.0466 0.715 0.399 0.328 N 10 0.1315 10 0.0362 10 0.0318 10 0.0136 10 10 FEW = FINAL BODY WEIGHT TABLE 33 PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 4 SPONSOR: HAP TASK FORCE INDIVIDUAL ORGAN WTS. RELATIVE TO FINAL BODY WTS. [G/ 100 G] WEEK 13 MALE GROUP: 100 PPM _____________________________________

ANIMAL FBW( G) ___ _____ .____________ 69304 323. 69307 327. 69318 308. 69319 305. 69327 303. 69329 293. 69332 293. 69334 288. 69342 306. 69346 303.

MEAN 305. S. D. 12.5 N 10 FBW = FINAL BODY WEIGHT NA = NOT APPLICABLE BRAIN 0.591 0.596 0.594 0.613 0.607 0.638 0.648 0.632 0.585 0.587 LIVER KIDNEYS __________ .________ 2.780 0.731 2.902 0.679 2.825 0.698 2.774 0.718 2.564 0.663 2.846 0.754 2.580 0.662 2.944 0.753 2.765 0.725 2.934 0.739 LUNGS HEART 0.402 0.416 0.393 0.416 0.403 0.413 0.379 0.413 0.373 0.393 .___ 0.331 0.346 0.321 0.308 0.304 0.324 0.331 0.337 0.288 NA 0.609 2.791 0.712 0.400 0.321 0.0228 0.1323 0.0348 0.0154 0.0182 10 10 10 10 9 PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 33 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL ORGAN WTS. RELATIVE TO FINAL BODY WTS. [G/ 100 G] PAGE 5 WEEK 13 GROUP: 0 PPM _______________

ANIMAL ________ 69302 69306 69308 69310 69312 69314 69317 69322 69337 69343 MALE __________________________________ EBIDID SPLEEN YMIDES ____________________________________~

0.180 0.325 0.182 0.337 0.206 0.355 0.205 0.375 0.209 0.329 0.203 0.320 0.191 0.294 0.179 0.336 0.194 0.377 0.232 0.346 ____ TESTES .______________ 0.941 1.086 1.074 1.057 1.085 1.060 1.013 1.068 1.092 1.076 _____________________ ADRENAL THYROIDS ___ THYMSJS____________ GLRNDS______________! P~~_ 0.059 0.015 0.006 0.056 0.020 0.007 0.065 0.022 0.007 0.070 0.021 0.004 0.066 0.020 0.005 0.062 0.017 0.005 0.067 0.013 0.003 0.060 0.022 0.005 0.066 0.021 0.008 0.057 0.020 0.007 MEAN 0.198 0.339 S. D. 1.055 0.0164 0.063 0.0253 0.019 N 0.0459 0.006 10 0.0047 10 0.0031 10 0.0016 10 10 10 PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 33 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL ORGAN WTS. RELATIVE TO FINAL BODY WTS. [G/ 100 Gl PAGE 6 WEEK 13 MALE GROUP: 15 PPM ______________________________________ EPIDID ADRENAL THYROIDS ANIMAL SPLEEN YMIDES TESTES THYMUS GLANDS /PARA _________________~

69305 0.188 0.368 1.165 0.060 0.023 0.008 69316 0.228 0.322 1.104 0.067 0.020 0.007 69320 0.196 0.300 0.972 0.065 0.017 0.005 69323 0.186 0.297 0.963 0.059 0.018 0.006 69326 0.201 0.326 1.015 0.067 0.016 0.007 69335 0.209 0.322 1.013 0.061 0.017 0.005 69336 0.200 0.313 0.967 0.067 0.021 0.005 69338 0.205 0.344 1.147 0.071 0.021 0.005 69345 0.212 0.352 1.042 0.073 0.019 0.005 69347 0.209 0.312 0.968 0.078 0.018 0.009 MEAN 0.203 0.326 1.036 0.067 0.019 0.006 S. D. 0.0123 0.0228 0.0770 0.0060 0.0022 0.0015 N 10 10 10 10 10 10 PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 33 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL ORGAN WTS. RELATIVE TO FINAL BODY WTS. [G/ 100 G] PAGE 7 WEEK 13 MALE GROUP: 40 PPM __________________~~~ EPIDID ADRENAL THYROIDS ANIMAL SPLEEN YMIDES TESTES THYMUS GLANDS /FARA 69303 0.184 0.314 1.051 0.038 0.019 0.006 69309 0.192 0.328 1.055 0.068 0.021 0.005 69313 0.206 0.310 1.010 0.073 0.019 0.005 69315 0.166 0.372 1.111 0.071 0.019 0.007 69324 0.210 0.324 0.967 0.063 0.018 0.005 69328 0.196 0.335 1.227 0.064 0.015 0.005 69339 0.189 0.326 1.037 0.067 0.021 0.006 69344 0.215 0.313 0.984 0.067 0.018 0.005 69348 0.205 0.339 1.082 0.062 0.019 0.008 69350 0.218 0.311 1.071 0.078 0.020 0.005 MEAN 0.198 0.327 1.060 0.065 0.019 0.006 S. D. 0.0159 0.0187 0.0735 0.0107 0.0017 0.0011 N 10 10 10 10 10 10 PROJECT NO.: WIL 417002 TABLE 33 SPONSOR: HAP TASK FORCE 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL ORGAN WTS. RELATIVE TO FINAL BODY WTS. [G/ 100 G] PAGE 8 WEEK 13 ANIMAL SPLEEN __________ ______________________ 69304 0.235 69307 0.199 69318 0.208 69319 0.216 69327 0.211 69329 0.212 69332 0.191 69334 0.208 69342 0.180 69346 0.188 MALE GROUP: 100 PPM _____________ EPIDID YMIDES TESTES .______________________________

0.337 1.084 0.324 1.012 0.308 1.003 0.331 1.082 0.323 1.017 0.345 1.017 0.379 1.041 0.337 1.063 0.314 0.984 0.320 0.990 ______________________________~ ADRENAL THYROIDS THYMUS GLANDS /PARA ___________________________

0.075 0.021 0.004 0.076 0.019 0.006 0.072 0.017 0.007 0.065 0.017 0.005 0.058 0.016 0.005 0.066 0.018 0.008 0.066 0.017 0.005 0.068 0.020 0.008 0.055 0.018 0.005 0.057 0.018 0.005 MEAN 0.205 0.332 S. D. 1.029 0.0159 0.066 0.0200 0.018 0.0364 0.006 N 0.0074 10 10 0.0015 10 0.0014 10 10 10 PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE ANIMAL 69354 69366 69367 69378 69384 69398 69404 69416 69419 69432 FBW( G) __________

196. 179. 196. 180. 168. 197. 199. 192. 193. 179. BRAIN 0.969 0.955 0.913 1.006 1.024 0.944 0.910 0.958 0.912 1.011 TABLE 33 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL ORGAN WTS. RELATIVE TO FINAL BODY WTS. [G/ 100 G]

LIVER KIDNEYS LUNGS __________________ _____ 2.724 0.689 0.495 2.464 0.709 0.520 2.367 0.694 0.490 2.700 0.706 0.572 2.798 0.679 0.565 2.396 0.670 0.462 2.417 0.. 704 0.497 2.688 0.729 0.510 2.539 0.679 0.497 2.754 0.749 0.525 FEMALE GROUP: 0 PPM HEART SPLEEN OVARIES    _ _  _ _ _ 0.332 0.330 0.316 0.361 0.357 0.345 0.342 0.349 0.363 0.380 0.250 0.047 0.246 0.049 0.265 0.038 0.244 0.054 0.262 0.060 0.244 0.051 0.251 0.051 0.260 0.054 0.249 0.055 0.285 0.052 THYMUS ____ __ 0.108 0.101 0.137 0.121 0.115 0.109 0.130 0.105 0.121 0.103 _________ ______ ADRENAL THYROIDS GLANDS /PARA _______ __________ 0.033 0.007 0.028 0.007 0.023 0.011 0.025 0.006 0.029 0.010 0.032 0.008 0.028 0.006 0.029 0.009 0.026 0.008 0.031 0.007 PAGE 9 WEEK 13 MEAN 188. 0.960 2.585 0.701 0.513 0.348 0.256 0.051 0.115 0.028 0.008 S. D. 10.5 0.0424 0.1651 0.0243 0.0339 0.0187 0.0128 0.0058 0.0120 0.0031 0.0017 N 10 10 10 10 10 10 10 10 10 10 10 FBW = FINAL BODY WEIGHT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 33 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL ORGAN WTS. RELATIVE TO FINAL BODY WTS. [G/ 100 G] PAGE 10 WEEK 13 FEMALE GROUP: 15 PPM ________________________________________ 1 ANIMAL FBW( G) BRAIN ADRENAL THYROIDS LIVER KIDNEYS LUNGS HEART SPLEEN OVARIES THYMUS GLANDS /PARA ___________    _   _ _ _ 69362 176. 69376 159. 69382 195. 69390 182. 69393 166. 69396 188. 69409 180. 69413 3.73. 69435 179. 0.943 1.075 0.918 0.956 1.090 0.936 1.000 1.081 0.961 2 2 2    _ _ _ _ _ _  _ _ _ _ _ _  _ _ 722 0.773 824 0.723 379 0.651 429 0.753 608 0.753 596 0.633 639 0.672 572 0.792 508 0.726 0.625 0.503 0.477 0.571 0.584 0.521 0.533 0.561 0.542 , 0 0 0 0 0 0 0 0 0 375 377 333 357 355 335 356 399 341 _  _ _ _   _ _ .___  ________ 0.301 0.077 0.264 0.062 0.236 0.051 0.280 0.047 0.247 0.052 0.250 0.056 0.239 0.051 0.272 0.064 0.207 0.043 0.130 0.039 0.006 0.118 0.035 0.008 0.086 0.030 0.007 0.120 0.031 0.007 0.106 0.033 0.020 0.091 0.032 0.008 0.111 0.032 0.008 0.115 0.038 0.007 0.095 0.029 0.008 MEAN 178. 0.996 2.586 0.720 0.546 0.359 0.255 0.056 0.108 0.033 0.008 S. D. 10.9 0.0686 0.1380 0.0557 0.0447 0.0217 0.0277 0.0103 0.0147 0.0035 0.0011 N 9 9 9 9 9 9 9 9 9 9 9 FBW = FINAL BODY WEIGHT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE ANIMAL 69359 69369 69370 69380 69388 69394 69426 69428 69433 69436 FBW( G) ___________ 169. 184. 187. 202. 175. 182. 193. 168. 177. 192. BRAIN 1.030 0.989 0.904 0.896 1.040 0.951 0.927 1.042 1.011 0.927 MEAN 183. 0.972 S. D. 11.0 0.0573 N 10 10 FBW = FINAL BODY WEIGHT TABLE 33 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 11 INDIVIDUAL ORGAN WTS. RELATIVE TO FINAL BODY WTS. [G/ 100 Gl WEEK 13 FEMALE GROUP: 40 PPM _____________ _. _________________________________

LIVER KIDNEYS LUNGS ______________.  _ _ _ _ _ _ 2.521 0.716 0.491 2.397 0.712 0.543 2.647 0.642 0.519 2.361 0.663 0.480 2.817 0.743 0.549 2.505 0.714 0.549 2.580 0.725 0.482 2.625 0.714 0.560 2.774 0.780 0.599 2.615 0.724 0.495 HEART SPLEEN OVARIES 0.361 0.260 0.056 0.337 0.228 0.045 0.342 0.246 0.049 0.327 0.228 0.043 0.400 0.280 0.069 0.352 0.264 0.049 0.347 0.244 0.050 0.375 0.280 0.048 0.373 0.260 0.060 0.323 0.224 0.039 THYMUS _ _ _ _ _ _ _ _ 0.123 0.108 0.099 0.115 0.113 0.119 0.103 0.111 0.119 0.108 ADRENAL THYROIDS GLANDS /FARA ________ ___________ 0.033 0.010 0.031 0.006 0.031 0.008 0.027 0.006 0.036 0.009 0.027 0.007 0.029 0.007 0.029 0.006 0.028 0.009 0.027 0.009 2.584 0.713 0.527 0.354 0.251 0.051 0.112 0.030 0.008 0.1460 0.0383 0.0396 0.0239 0.0207 0.0088 0.0075 0.0030 0.0015 10 10 10 10 10 10 10 10 10 PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE ANIMAL FBW( G) BRAIN _______________________ 69375 171. 1.018 69379 181. 0.989 69383 178. 1.006 69385 187. 0.925 69392 165. 1.055 69402 186. 0.925 69408 181. 0.994 69415 183. 0.984 69427 178. 0.983 69431 181. 0.983 MEAN 179. 0.986 S. D. 6.7 0.0390 N 10 10 w E FEW = FINAL BODY WEIGHT R m s .

. TABLE 33 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 12 INDIVIDUAL ORGAN WTS. RELATIVE TO FINAL BODY WTS. [G/ 100 GI WEEK 13 FEMALE GROUP: 100 PPM ________________________________________

LIVER LUNGS HEART __________ 2.749 2.751 2.742 2.626 2.685 2.849 2.691 2.667 2.747 2.713 KIDNEYS ___ 0.731 0.757 0.719 0.786 0.788 0.742 0.773 0.705 0.736 0.812 ____ 0.520 0.351 0.613 0.359 0.579 0.376 0.476 0.390 0.533 0.364 0.516 0.355 0.552 0.370 0.530 0.415 0.618 0.371 0.575 0.398 SPLEEN _ _  _ _ _ _ 0.246 0.254 0.258 0.241 0.236 0.242 0.254 0.246 0.264 0.271 OVARIES  _ _ _ _ _ _ 0.048 0.068 0.053 0.051 0.046 0.050 0.053 0.060 0.060 0.056 ADRENAL THYROIDS THYMUS GLANDS /PARA _________________________________ 0.119 0.030 0.009 0.119 0.035 0.009 0.101 0.032 0.008 0.110 0.029 0.006 0.114 0.031 0.006 0.113 0.029 0.005 0.093 0.030 0.006 0.123 0.029 0.010 0.142 0.035 0.011 0.113. 0.036 0.009 2.722 0.755 0.551 0.375 0.251 0.055 0.115 0.032 0.008 0.0609 0.0342 0.0451 0.0204 0.0110 0.0066 0.0131 0.0028 0.0020 10 10 10 10 10 10 10 10 10 POFBWv4.1 01/ 18/ 2002 R: 01/ 18/ 2002 PROJECT NO.: WIL 417002 TABLE 34 SPONSOR: HAP TASK FORCE 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL ORGAN WEIGHTS RELATIVE TO BRAIN WEIGHTS [G/ 100 G] PAGE 1 WEEK 13 ______________________________________~~ BRAIN WT ANIMAL FBW( G) (GRAMS) ________________________ 69302 305. 1.83 69306 291. 1.81 69308 282. 1.88 69310 264. 1.73 69312 316. 1.88 69314 281. 1.74 69317 309. 1.84 69322 280. 1.85 69337 284. 1.84 69343 263. 1.83 MALE GROUP: 0 PPM ______________________________________

 _ _ _ _ _ _ _ LIVER ______ 468.306 455.801 446.809 424.855 450.000 430.460 454.893 402.162 436.413 426.230 KIDNEYS _______________ 103.279 115.470 104.787 108.092 114.894 107.471 109.783 108.108 104.348 98.361 MEAN 288. 1.82 439.593 S. D. 17.9 107.459 0.051 65.419 19.3561 51.290 N 10 5.1934 10 2.9922 10 3.1086 10 10 10 FBW = FINAL BODY WEIGHT _________________

LUNGS . _____. 68.306 63.536 58.511 66.474 66.489 68.391 65.761 68.108 64.130 64.481 HEART ._______________ 54.098 49.724 52.128 54.913 53.723 48.851 48.370 47.027 55.435 48.634 PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 34 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL ORGAN WEIGHTS RELATIVE TO BRAIN WEIGHTS [G/ 100 Gl ANIMAL FBW( G) __________ c__

69305 261. 69316 307. 69320 317. 69323 323. 69326 334. 69335 320. 69336 300. 69338 273. 69345 307. 69347 282. MALE GROUP: 15 PPM _________

BRAIN WT (GRAMS) LIVER KIDNEYS ________________ 1.87 380.749 100.000 1.92 509.375 117.188 1.87 467.914 117.647 1.84 480.978 114.674 1.98 454.040 110.606 1.95 454.359 114.872 1.86 468.280 111.828 1.87 386.096 96.791 2.01 430.846 113.930 1.76 455.682 113.068 MEAN 302. 1.89 448.832 111.060 S. D. 23.5 0.073 40.0560 7.0516 N 30 10 10 10 FBW = FINAL BODY WEIGHT PAGE 2 WEEK 13 LUNGS HEART __________________ .____ 51.337 43.316 69.792 52.083 74.866 53.476 71.196 53.804 70.707 49.495 64.615 55.385 65.054 53.226 59.358 47.059 63.184 48.756 69.886 49.432 66.000 6.8888 10 50.603 3.6834 10 PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE SPONSOR: HAP TASK FORCE INDIVIDUAL ORGAN WEIGHTS RELATIVE TO BRAIN WEIGHTS [G/ 100 G] PAGE 3 WEEK 13 MALE GROUP: 40 PPM ______________________________________ TABLE 34 BRAIN WT ANIMAL FBW( G) (GRAMS) LIVER KIDNEYS LUNGS HEART  _    _ _ _  69303 69309 69313 69315 69324 69328 69339 69344 69348 69350 ________________

255. 308.

310.

253. 1 1 333.

322. 270.

316.

292, 280. 1 1 1 1 1 1 .____^________

61 89 91 85 95 90 77 84 92 88 MEAN 294. 1.85 S. D. 28.3 0.099 N 10 10 ____ 397.516 424.339 471.728 358.919 459.487 452.105 420.339 489.130 435.417 387.234 ______________________ 109.317 129.101 118.325 94.054 114.872 115.789 109.605 126.087 113.021 103.723 __

61 73 63 56 64 68 50 67 61 63 _______________ 491 545 874 757 103 421 847 391 979 830 _______

55.280 51.323 53.927 47.027 51.795 55.789 51.412 54.891 49.479 48.936 429.621 113.389 63.224 51.986 40.3947 10.2130 6.2541 2.9534 10 10 10 10 w" FBW = FINAL BODY WEIGHT ;; '

2 5; PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 34 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL ORGAN WEIGHTS RELATIVE TO BRAIN WEIGHTS [G/ 100 G] PAGE 4 WEEK 13 MALE GROUP: 100 PPM ________________~ BRAIN WT ANIMAL FBW( G) (GRAMS) LIVER KIDNEYS LUNGS HEART ___________________________________ 69304 323. 1.91 470.157 123.560 68.063 56.021 69307 327. 1.95 486.667 113.846 69.744 57.949 69318 308. 1.83 475.410 117.486 66.120 54.098 69319 305. 1.87 452.406 117.112 67.914 50.267 69327 303. 1.84 422.283 109.239 66.304 50.000 69329 293. 1.87 445.989 118.182 64.706 50.802 69332 293. 1.90 397.895 102.105 58.421 51.053 69334 288. 1.82 465.934 119.231 65.385 53.297 69342 306. 1.79 472.626 124.022 63.687 49.162 69346 303. 1.78 499.438 125.843 66.854 NA MEAN 305. 1.86 458.881 117.063 65.720 S. D. 12.5 0.054 30.4098 7.2201 3.1061 N 10 10 10 10 10 FBW = FINAL BODY WEIGHT NA = NOT APPLICABLE 52.517 3.0131 9 PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 34 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL ORGAN WEIGHTS RELATIVE TO BRAIN WEIGHTS [G/ 100 G] PAGE 5 WEEK 13 MALE GROUP: 0 PPM _____________________~~~~~~~~~~~~ EPIDID ADRENAL ANIMAL THYROIDS SPLEEN YMIDES TESTES THYMUS GLANDS /PARA _______________________~~~~~~~~~~~

69302 30.055 54.098 156.831 9.913 2.464 69306 1.077 29.282 54.144 174.586 8.945 3.287 69308 1.171 30.851 53.191 161.170 9.761 69310 3.335 1.106 31.214 57.225 161.272 10.607 3.156 69312 0.647 35.106 55.319 182.447 11.037 69314 3.394 0.787 32.759 51.724 171.264 10.000 69317 2.799 32.065 0.868 49.457 170.109 11.250 69322 2.136 0.571 27.027 50.811 161.622 9.027 3.254 69337 0.789 29.891 58.152 168.478 10.228 3.217 69343 33.333 1.239 49.727 154.645 8.158 2.831 1.049 MEAN 31.158 53.385 166.242 9.893 S. D. 2.987 2.2902 0.930 2.9912 8.6425 0.9696 0.4199 N 0.2294 10 10 10 10 10 10 PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 34 YO DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL ORGAN WEIGHTS RELATIVE TO BRAIN WEIGHTS [G/ 100 G]

MALE GROUP: 15 PPM ______________________________________ EPIDID ANIMAL SPLEEN YMIDES TESTES THYMUS ________________________________________

69305 26.203 51.337 162.567 8.417 69316 36.458 51.563 176.563 10.703 69320 33.155 50.802 164.706 10.947 69323 32.609 52.174 169.022 10.315 69326 33.838 55.051 171.212 11.333 69335 34.359 52.821 166.154 10.087 69336 32.258 50.538 155.914 10.758 69338 29.947 50.267 167.380 10.364 69345 32.338 53.731 159.204 11.214 69347 33.523 50.000 155.114 12.557 MEAN 32.469 51.828 164.784 10.670 3.014 0.980 S. D. 2.7617 1.6303 6.7896 1.0550 0.2286 0.2459 N 10 10 10 10 10 10 PAGE 6 WEEK 13 ADRENAL THYROIDS GLANDS /PARA ________________________ 3.246 1.139 3.276 1.083 2.872 0.909 3.136 0.967 2.631 1.258 2.774 0.744 3.323 0.785 3.016 0.781 2.920 0.692 2.949 1.438 PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 34 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL ORGAN WEIGHTS RELATIVE TO BRAIN WEIGHTS [G/ 100 G] PAGE 7 WEEK 13 MALE GROUP: 40 PPM _____________________________________

ANIMAL SPLEEN EPIDID YMIDES ADRENAL THYROIDS TESTES THYMUS GLANDS /PARA _______________________ ________________ 69303 29.193 69309 31.217 69313 33.508 69315 22.703 69324 35.897 69328 33.158 69339 28.814 69344 36.957 69348 31.250 69350 32.447 49.689 53.439 50.262 50.811 55.385 56.842 49.718 53.804 51.563 46.277 _____  _____ 166.460 6.037 171.958 11.079 163.874 11.874 151.892 9.697 165.128 10.810 207.895 10.789 158.192 10.164 169.022 11.549 164.583 9.448 159.574 11.543 3.075 3.460 3.131 2.562 3,082 2.516 3.232 3.054 2.943 3.011 _  _ _ _ _ _ _

0.894 0.799 0.848 1.016 0.836 0.858 0.932 0.804 1.229 0.681 MEAN 31.514 51.779 167.858 10.299 3.007 0.890 S. D. 4.0395 3.1210 15.1808 1.6987 0.2842 0.1482 N 10 10 10 10 10 10 PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 34 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL ORGAN WEIGHTS RELATIVE TO BRAIN WEIGHTS [G/ 100 G] PAGE 8 WEEK 13 MALE GROUP: 100 PPM _________________________________^__~ EPIDID ADRENAL THYROIDS __"" r"""____________________ s"""""____________ '" '"" s_____________ T"" T""____________ 'f" '" 51s___________~~~~~~~~~!~~~

69304 39.791 57.068 183.246 12.764 3.529 0.733 69307 33.333 54.359 169.744 12.682 3.267 1.010 69318 34.973 51.913 168.852 12.044 2.940 1.246 69319 35.294 54.011 176.471 10.535 2.717 0.813 69327 34.783 53.261 167.391 9.576 2.565 0.902 69329 33.155 54.011 159.358 10.299 2.866 1.198 69332 29.474 58.421 160.526 10.232 2.626 0.789 69334 32.967 53.297 168.132 10.819 3.143 1.236 69342 30.726 53.631 168.156 9.402 3.140 0.939 69346 32.022 54.494 168.539 9.787 2.994 0.893 MEAN 33.652 54.447 169.042 10.814 2.979 0.976 S. D. 2.8460 1.9110 6.8990 1.2502 0.3014 0.1901 N 10 10 10 10 10 10 TABLE 34 PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 9 SPONSOR: HAP TASK FORCE INDIVIDUAL ORGAN WEIGHTS RELATIVE TO BRAIN WEIGHTS [G/ 100 Gl WEEK I. 3 FEMALE GROUP: 0 PPM _______________________________________~ BRAIN WT ADRENAL THYROIDS ANIMAL FBW( G) (GRAMS) LIVER KIDNEYS LUNGS HEART SPLEEN OVARIES THYMUS GLANDS /PARA ____________________________________ 69354 196. 1.90 281.053 71 69366 179. 1.71 257.895 74 69367 196. 1.79 259.218 75 69378 180. 1.81 268.508 70 69384 168. 1.72 273.256 66 69398 197. 1.86 253.763 70 _____________________________ 053 51.053 34.211 25 269 54.386 34.503 25 978 53.631 34.637 29 166 56.906 35.912 24 279 55.233 34.884 25 968 48.925 36.559 25 ________ 789 4.858 11.105 3 731 5.175 10.608 2 050 4.156 14.983 2 309 5.354 12.011 2 581 5.849 11.227 2 806 5.435 11.591 3 395 0.674 883 0.766 553 1.207 481 0.602 855 0.948 435 0.833 69404 199. 1.81 265.746 77.348 54.696 37.569 27.624 5.624 14.293 3.133 0.702 69416 192. 1.84 280.435 76.087 53.261 36.413 27.174 5.592 10.929 3.011 0.984 69419 193. 1.76 278.409 74.432 54.545 39.773 27.273 6.017 13.227 2.875 0.915 69432 179. 1.81 272.376 74.033 51.934 37.569 28.177 5.105 10.149 3.105 0.729 MEAN 188. 1.80 269.066 73.061 53.457 36.203 26.651 5.317 12.012 2.973 0.836 S. D. 10.5 0.059 9.7528 3.3849 2.2934 1.7570 1.4385 0.5356 1.6240 0.3138 0.1806 N 10 10 10 10 10 10 10 10 10 10 10 FBW = FINAL BODY WEIGHT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 34 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL ORGAN WEIGHTS RELATIVE TO BRAIN WEIGHTS [G/ 100 G] PAGE 10 WEEK 13 ANIMAL 69362 69376 69382 69390 69393 69396 69409 69413 69435 FBW( G) ._ _______ 176. 159. 195. 182. 166. 188. 180. 173. 179,    _  _    BRAIN WT (GRAMS)    _     _ 1.66 1.71 1.79 1.74 1.81 1.76 1.80 1.87 1.12 LIVER KIDNEYS LUNGS HEART _____ ______ _______ ___________ 288.554 81.928 66.265 39.759 262.573 67.251 46.784 35.088 259.218 70.950 51.955 36.313 254.023 78.736 59.770 37.356 239.227 69.061 53.591 32.597 277.273 61.614 55.682 35.795 263.889 67.222 53.333 35.556 237.968 73.262 51.872 36.898 261.046 75.581 56.395 35.465 FEMALE GROUP: 15 PPM ________ ________________

SPLEEN .___ ___ 31.928 24.561 25.698 29.310 22.652 26.705 23.889 25.134 21.512 ____________________________________ ADRENAL THYROIDS OVARIES THYMUS GLANDS /PAR?+ _____________________________________

8.139 13.783 4.139 0.620 5.760 10.982 3.263 0.749 5.570 9.374 3.296 0.721 4.931 12.552 3.241 0.724 4.801 9.718 3.039 0.906 5.960 9.750 3.415 0.835 5.067 11.094 3.200 0.844 5.963 10.636 3.508 0.642 4.471 9.913 3.017 0.849 MEAN 178. 1.76 260.419 72.401 55.072 36.092 25.710 5.629 10.867 3.346 0.766 S. D. 10.9 0.063 16.1563 5.3767 5.5152 1.9279 3.2511 1.0823 1.4649 0.3362 0.0988 N 9 9 9 9 9 9 9 9 9 9 9 2 FBW = FINAL BODY WEIGHT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 34 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL ORGAN WEIGHTS RELATIVE TO BRAIN WEIGHTS [G/ 100 Gl PAGE 11 WEEK 13 ANIMAL _ _ _  _ _ _ _ 69359 69369 69370 69380 69388 69394 69426 69428 69433 69436 BRAIN WT FBW( G) (GRAMS)

169. 1.74 184. 1.82 187. 1.69 202. 1.81 175. 1.82 182. 1.73 193. 1.79 168. 1.75 177. 1.79 192. 1.78 LIVER KIDNEYS LUNGS HEART __________ __ ______   _ _ _   244.828 69.540 47.701 35.057 242.308 71.978 54.945 34.066 292.899 71.006 57.396 37.870 263.536 74.033 53.591 36.464 270.879 71.429 52.747 38.462 263.584 75.145 57.803 36.994 278.212 78.212 51.955 37.430 252.000 68.571 53.714 36.000 274.302 77.095 59.218 36.872 282.023 78.090 53.371 34.831 FEMALE GROUP: 40 PPM ___________________________ ______

SPLEEN 25.287 23.077 27.219 25.414 26.923 27.746 26.257 26.857 25.698 24.157 OVARIES THYMUS _____ __ ___________^_ 5.420 11.937 4.566 10.912 5.467 10.899 4.807 12.873 6.588 10.879 5.150 12.503 5.441 11.095 4.617 10.703 5.933 11.788 4.213 11.663 ADRENAL THYROIDS GLANDS /PARA 3.201 0.948 3.181 0.621 3.420 0.929 3.011 0.680 3.500 0.846 2.821 0.699 3.089 0.715 2.829 0.617 2.726 0.933 2.904 0.972 MEAN 183. 1.77 266.457 73.510 54.244 36.405 25.864 5.220 11.525 3.068 0.796 S. D. 11.0 0.043 16.4571 3.5274 3.3266 1.4113 1.4485 0.7079 0.7511 0.2596 0.1434 N 10 10 10 10 10 10 10 10 10 10 10 k? FBW = FINAL BODY WEIGHT \o % 0 l s; PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE TABLE 34 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL ORGAN WEIGHTS RELATIVE TO BRAIN WEIGHTS [G/ 100 Gl PAGE 12 WEEK 13 ANIMAL 69375 69379 69383 69385 69392 69402 69408 69415 69427 69431 __________________

BRAIN WT FEW( G) (GRAMS) _______________

171. 1.74 181. 1.79 178. 1.79 187. 1.73 lb5. 1.74 186. 1.72 181. 1.80 183. 1.80 178. 1.75 181. 1.78 LIVER KIDNEYS _________ __________

270.115 71.839 278.212 76.536 272.626 71.508 283.815 84.971 254.598 74.713 308.140 80.233 270.556 77.778 271.111 11.667 279.429 74.857 275,843 82.584 FEMALE GROUP: 100 PPM __________ ^_______________________________

LUNGS HEART ____________ ______ 51.149 34.483 62.011 36.313 57.542 37.430 51.445 42.197 50.575 34.483 55.814 38.372 55.556 37.222 53.889 42.222 62.857 37.714 58.427 40.449 SPLEEN OVARIES THYMUS 24.138 4.690 11.690 25.698 6.832 11.989 25.698 5.318 10.039 26.012 5.497 11.925 22.414 4.385 10.764 26.163 5.424 12.209 25.556 5.322 9.339 25.000 6.122 12.472 26.857 6.069 14.451 27.528 5.697 11.281 .____________ ADRENAL THYROIDS GLANDS /PARA 2.902 0.920 3.536 0.883 3.196 0.760 3.162 0.676 2.920 0.598 3.145 0.576 3.044 0.589 2.983 1.011 3.537 1.131 3.624 0.910 MEAN 179. 1.76 276.445 76.669 55.927 38.089 25.506 5.536 11.616 3.205 0.805 S. D. 6.7 0.031 13.6070 4.7135 4.3431 2.7907 1.4285 0.7056 1.4092 0.2686 0.1943 N 10 10 10 10 10 10 10 10 10 10 10 FBW = FINAL BODY WEIGHT POFBWv4.1 % 01/ 18/ 2002 o\ R: 01/ 18/ 2002 s WIL 4 17002 HAP Task Force 1, 1,2 Trichloroethane APPENDIX A Analysis of Test Article Samples (Sponsor Provided)

341 of 619 Dow USA Texas Operations The Dow Chemi, al Company 2301 Brazospoti Btvd Freeport, Texas 77541 3257 July 27, 200 1 WIL Research Laboratoties, Inc. ATTN: qeter Voytek 1407 George +d Ashland, OH 44805 928 1 P& z Anaiysis of 1,1,2 Trichloroethane samples Dear Mr. Voytek I received two samples of l, l, Z trichloroethane from you on the afternoon of 7/ 25/ 01. Reference numbers for the samples are: WE417001 and WIL 417002. Tbe samples were returned for assay or determination of the purity of the 1,1,2 Uichloroetha. The samples were analyzed that same afternoon in our Quality Assurance lab which dws the analytical work for the release of our product. The results are as foliows:

1,1,2 trichloroetbane 1 , 1,1.2 Tetrachloroethane 1,2 ButyIene Oxide Secondary Butanof wIL 417001 99.546 Wt % 0.0600 Wt % Ct. 1893 WI % 0.2047 Wt % `NIL417002 99.549 wt % 0.0588 wt % normal process impurity 0.1895 wt % stabilizer 0.2023 Wt % stabilizer This material is Stabilized 1,1,2 Trichloruethane. The 1,2 butytene oxide serves as an acid acceptor and provides stability against metals. The secondary butanol sewes to provide stability against metak.

The analysis was done by gas chromatography. The method is as follows:

column Temperature program DB 1701 I p film thickness, 0.32 mm X 60 meter Initial Temp of 40° C for 3 min 10" Chin to 200° C stop carrier gas Heiium at a constant flow of 3 ccimin Split ratio 2OO: l As you know, Dow is a producer of I, 1 &richloroethane and producer of the material being analyzed I believe. We routinely analyze this materia1. We calibrate the gas chromatographs with standards containing the known process impurities and stabilizers in concentrations in the range that these impurities and srandards are normally present. We prepare the standards from pure components using an analytical balance. We determine the concentration of impurities and stabilizers present and then determine assay of the 1 ,I ,Z trichloroethane by subtracting the sum of the impurities and stabilizers from 100%. II is probably quite obvious that this method gives greater precision than does measuring the concentration of the 1.1,2 bichloroethane dir& y. The foIlowing simple example illustrates this. Let us assume that the relative standard deviation of the gas chromatogmphic analysis is ~3%. It is certainly obvious then that the resuIts of direct measuremcm of the component that is almosr 100% of the total will have a standard deviation of about 3%.

Now let us assume that same 3% RSD applies to each of the three measured components then the combined relative standard deviation for the three components is:

342of619 RSD, = d= 5.2%

5.2% of a total of about 0.45 wt % stabilizer plus impurity is about i0.023% compared to 23% for direct measurement.

I am enclosing copies of the GC scans. Note the following internal slang terms and short cuts as seen on the GC scam and computer prints outs.

BO = ! 1.2 Butylene Oxide SBA SC Secondary Butanol 1112, tetra s 1, I, I &tetrachloroethane B Tri (Beta Td or B Tri) = I, 1 ,Z trichloroethane Acetone is not a process impurity but a rinse solvent between sample vials on the autoinjector. Most of the small area counts seen on the computer printout is baseline noise as can bc seen by comparing it to the scan. The product can also contain trace amounts (< O. O1 WI %) of ethylene dichloride, trichloroethylene and bromochloroethane,

If I can be of further assistance pkase do not hesitate to contact me.

a+#?

Douglas L. Teny Chlorinated Organic Products Technologies Center Building A 2422 Office: 979 238 4232 Pager 888437 6791 FAX: 979 238 0136 E Mail dougteny@ dow. com 343 of 619 Sample Worksheet : 56260 Page 1 ____ I SOLVENTS SAMPLE WORKS4EETI __ I___ I________________

/ Worksheet prepared on 25 JUL 2001.

Sample ID : 56260 Text ID : B VU S JUL 25 52 Comments : #5015A fl  L C PRODUCT : B TRI S SPEC LEVEL : PRODUCTION SYIPMENT LOT NUMBER : CARRIER ID : SAMPLE TYlE : TEST COMPARTMENT : LOGIN DATE : 25 JUL 2001 18: 36 LOGIN BY : u137519 ========================== s== 2==~==~==========

The foilowing tests have been. assigned to this sample:  c_~___~~~~~~~~~~~~~~~~~~~~~~~~

___ C_______ I Analysis I Component Name I MIN I MAX I Result 1 Units 1 , c +++++, I ASSAY? 3TRI/ I BO jo. 100 10.200 IO. 1893 I %wt I , _ ++++~, I I saA 10.200 IO. 350 (0.2047 I %wt i /+ f~+~, I 1 1112, Tetra I I 10.0600 %wt I + ++ i+ I I j B Tri I I 199.546 I %wt (++~* + I The Dow Chemical Company Dow Confidential 344of619 . Sample Worksheet : 56260 Page 1 1 SOLVENTS SAMPLE WORKSHEET] _______________^________________

Worksheet prepared on 25 JCJL 2001.

Sample ID : 56260 Text ID : B TRI S JUL 25 52 Comments : #5015A lfl  PRODUCT : B TRI S SPEC LEVEL : PRODUTTION SHIPMENT : LOT NUMBER : SAMPLE TYPE : TEST LOGZN DATE : 25 m 2001 18: 36 CARRIER ID : COMPARTMENT : LOGIN BY : u137519 The foll. owing tests have been assigned to this sample: _____________ r____________

________ _________ c______

Analysis 1 Component Name I MIN / MAX [ Result [ Units +*__________ f___ + i+ ASSAY BTRI/ l/ BO jo. roo ~0.200 1  f+~~ Gf 25 ___ !_"~ r~~~ ( SBA 10.200 lo. 350 \ ;I GV7 I %Wt *+~ +.+ [ 1112, Tetra I I I I %wt + 06 f++ '+ 1 B Tri I I 1 $jT jq6 I %wt ,++~*~~

The Dow Chemical Company Dow Confidential 345 of 619 .[ TERMIITAL] 191 BTR25jULO11845,1,1 Reported on 25 JUL 2001 at 19: 06 Page 1 Injection Report Acquired on 25 JUL 2001 at 18: 48 TX% 02 Texas Division Manufacturing Labs Analyst Name : REED Lims Id : ASSAY BTRI Comment : #5015# 1 Method Title : B Tri (1,1,2 Trichloroethane) Sample Name L B Tri (1,1,2 Trichloroethane) Sample Id : 56260 Sample Type : Sample Amount= l. OOOOO Bottle No : 1 v

PEAK INFORMATION 346of619 TXVG02 Texas Divis f& a Lys i s Name : CTERMINFILI ELTrl I1.1,2 Trlchl. oroethane)

Channel 191 B Tr I 90 811 E 5 on Mar w fact ur i ng Labs 191 !3TR2S& tL011845,1~ 1.

Rmount I 1 .cIcIO 1.

Ineti umant II HP5890 13 Channe I Tit le a Channel ~193 Llms ID 8 56260 Acquired on 25 JUL 2001 at 18~ 48 Reported on 25 JUL 2001 at IPSO6 Met; hod s B TRI Callbrdtion s B TRI Run Sequence s B TRI Sample Worksheet : 56261 Page '1 _ I SOLVENTS SAMPLE WORKSHESTI _

Worksheet prepared on 25. TUL 2001.

SamFle ID I 56261 Comments : #5015A #2 Text ID : B TRI S X? L 25 53 _L

PRODUCT : B TRI S SPEC LEVEL : PRODUCTION SHIPMENT LOT NUMBER .: CARRIER ID : SA??? LE TYPE : TEST COMPARTMENT : LOGIN DATE : 25 JUL 2001 18: 38 LOGIN BY : u137519 ================ r================ n=================~=======~===========

The following tests have been assigned to this sample: ~

1 Analysis I Component Name I MIN l w4. x I Result I Units i i  f  s+++ 1 I ASSAY BTRI/ I BO 10.100 10.200 IO. 1895 %wt ,  +  r++++* I 1 I SBA 10.200 10.350 10.2023 / %wt I 1+ f+ ++, \ I 113.2, Terra I I 10.3588 I %wt 1 , +++~~~ i+~+ I j B Tri I, i ' 199.549 I %wt I +++ I The Dow Chemical Company DOW Confidential 348 of 619 Sample Worksheet : 55261 Page I _______ c_^______________

/ SOLVENTS SAMPLE WORKSHEET1 ____________ L________________

Worksheet prepared on 25 J~ JL 2001..

Sample ID : 56261 Text ID : B TRI S m 25 53 Comments : #5015A #2 __________ L_ r_______

PRODUCT : B TRI s SPEC LEVEL : PEODU? TION SHIPMENT . LOT NUMBER . SAMPLE TYPE I TEST CARRIER ID 1 COMPARTMENT : LOGIN DATE : 25 JUL 2001 l. 8: 38 LOGIN BY : u13 7519 The following tests have been assigned to this sample: ____ ti 1 halysis j Component Name 1 MIN I  I Result 1 Units * ASSAY BTRI/ l\ BO c++* Jo. 100 jo. 200 1 %wt ~+ i, JPYs' ++ 4 . g.

] SBA 10.200 10.350 1 %wt .* ~ c+ ';+~* 3c3'3 1 1112, Tetra +" [ B Tri + f*

The Dow Chemical Company Dow Confidential 349of619 '[ TERMINAL! 191 BTR25JUL011919,1,1 Reported on 25 JUL 2001 at 19: 41 Page 1 Injection Report Acquired on 25 J7UL 2001 at 19: 22 TXVG02 Texas Division Manufacturing Labs Analyst Name : ED Lims Id : ASSAY BTRI Comment : #S015A #2 Method Title : B Tri (1,1,2 Trichloroethane) Sample Name : B Tri (1,1,2 Trichloroethane) Sample Id : 56261 Sample Type : Sample Amount= l. 00000 Bottle No : 1 350 of 619 TXVG02 Texas Q i v i s i on Maw f& t ur i t g Labs Hna 1 p i s Name : [TERM 1 NRLI 19 1 BTR2SJULO 119 I9,1,1,

B Tr I (1.2.2` Tr Ich loroethanel Rmount I 1.000 Channel 191 B Tr I E 5 3 m 5 "r 70 `50 i I

50  50

40  40

30  30  t t a a w w 20  20  ul ul 2 2 10 10 A.. A.. I I t I I I L I I t I I I L 0.0 0.0 2.0 2.0 Time (mlnutesl Hult I& ram 5

.P Instrument g HP5890 A Channel Tit te g Channel tr. lPl Llms ID .a 56261 Acquired on 25 JUL 2001 at 19~ 22 Reported on 25 JUL 2001 at 79g41 Het hod n B TRl Callhrat Ion P R TRI Run Seqtience s B TRI ~TERMIw& I 193. BTR25SJLclli919,1,1 Reported on 25 JUi 2001 at 19: 41 Page 2

ANALYSIS SUMMARY Method.......................... B TRI Run sequence.................... B TRI Calibration..................... B TRI Normalised % calibration using area Calibration last modified on 25~ PJL 2001 at 19: 07 Method last modified on ll JAN 2001 at i5: 34 Uncalibrated peaks use user factor (Q. 0000) WIL 4 17002 HAP Task Force APPENDIX B 1, 1,2 Trichloroethane Purity Assessment (WIL Research Laboratories, Inc.)

353 of619 WIL 4 17002 HAP Task Force Page 1 A 90 DAY INHALATION TOXICITY STUDY OF 1,1,2 TRICHLOROETHANE (l, l, ZTCE) IN RATS (WITH SATELLITE GROUPS FOR PHARMACOlKENETIC EVALUATIONS IN RATS AND MICE)

Purity Assessments Analytical Chemistry Department WIL Research Laboratories, Inc.

354 of 619 WIL 417002 HAP Task Force Page 2 A 90 DAY INHALATION TOXICITY STUDY OF 1,1,2 TRICHLOROETHANE (1,1,2 TCE) IN RATS (WITH SATELLITE GROUPS FOR PHARMACOKENETIC EVALUATIONS IN RATS AND MICE)

TABLE OF CONTENTS m I. INTRODUCTION 4 II. EXPERIMENTAL 4 A. Gas Chromatography B. Preparation of Samples for Purity Analysis 5 C. Purity Assessment 5 III. CONCLUSION 7 355 of 619 WIL 417002 HAP Task Force Page 3 A 90 DAY INHALATION TOXICITY STUDY OF 1,1,2 TRICHLOROETHANE (l, l, ZTCE) IN RATS (WITH SATELLITE GROUPS FOR PHARMACOKENETIC EVALUATIONS IN RATS AND MICE)

INDEX OF FIGURES m Figure 1: Representative chromatogram of a commercially available (Aldrich Chemical Co.) 1,1,2 TCE reference standard 6 Figure 2: Representative chromatogram of a test article container sample 6 INDEX OF TABLES m I. Purity Assessments 8 356 of 619 WIL 417002 HAP Task Force Page 4 A 90 DAY INHALATION TOXICITY STUDY OF 1,1,2 TRICHLOROETHANE (1,1,2 TCE) IN RATS (WITH SATELLITE GROUPS FOR PHARMACOKENETIC EVALUATIONS IN RATS AND MICE)

Purity Assessments I. INTRODUCTION Bulk samples of test article (1 ,1,2 TCE) were analyzed to determine the test article purity by gas chromatography with flame ionization detection (GUFID).

Retention samples (taken from the test article container prior to its initial use on the study) and residual samples (taken from the test article container after its final use on the study) were analyzed to assess purity and stability.

The test article samples that were analyzed and used for inhalation exposures were greater than 99.0% pure. Comparison of the purity of samples taken at the beginning and end of use of the container demonstrated that the test article was stable for the duration of use on this study.

II. EXPERIMENTAL A. Gas Chromatography Instrument: Hewlett Packard 6890A (series 11) gas chromatograph equipped with a flame ionization detector (FID), an HP7673 autosampler, and an IIP ChemStation data system, or equivalent system Column: J & W Scientific DB wax 30 m x 0.25 mm ID, .25 pm film thickness Temperature Program: 40° C for 4.0 minutes; ramp at lO" C/ minute to 225° C; hold 0.0 minute 357 of 619 WIL 4 17002 HAP Task Force Page 5 Carrier gas: Helium at 6.58 psi at 40° C Injector temperature:

Injection volume:

Detector:

Retention time: 250° C Purity Check: 1 FL; split (67: l)

FID at 250° C Approximately 13.4 to 14.0 minutes for 1,1,2 TCE B. Preparation of Samples for Purity Analysis Bulk samples of test article (1,1,2 TCE) were analyzed to determine the test article purity by gas chromatography with flame ionization detection (GCFID).

Samples were analyzed to assess purity by transferring approximately 1 mL of test article into amber autosampler vials. The vials were capped and an aliquot (1 pL) injected for GC/ FID analysis.

C. Purity Assessment Samples were collected at the beginning and end of use from the test article container. They were transferred to Analytical Chemistry for GC/ FID analysis.

At least duplicate injections were made from each sample. The purity of the injected test article was calculated based on the area percent of the resulting peaks.

Purity assessment results are presented in Table I. These data indicate that the test article used in this study was 99.14% pure (mean of the before and after use purity assessments), with the replicate analytical results ranging from 98.97% to 99.39%. Representative chromatograms of a commercially available (Aldrich Chemical Co.) 1,1,2 TCE reference standard (98.08% pure) and a test article container sample are shown in Figures 1 and 2.

Comparison of the beginning of use purity assessment (99.0% pure) with the end of use assessment (99.3% pure) indicates that the test article was stable during room temperature storage over the duration of the study.

358 of 619 WIL 417002 HAP Task Force r FIDI B, (417002A\ SIG20003. D) Page 6 P 4 14 oi P E 5 i5 i0 Figure 1: Representative chromatogram of a commercially available (Aldrich Chemical Co.) 1,1,2 TCE reference standard FIDI 5, (417002A\ SIG20006. D)

Figure 2: Representative chromatogram of a test article container sample 359 of 619 WIL 4 17002 HAP Task Force Page 7 III. CONCLUSION The test article (1,12 TCE) was analyzed to assess test article purity and storage stability. The test article used for inhalation exposures was greater than 99.0% pure. Comparison of the beginning of use purity assessment (99.0% pure)

with the end of use assessment (99.3% pure) indicates that the test article was stable during room temperature storage over the duration of the study.

Prepared by:

e SL. / Cathline L. Shayq /,,` I+@? Leader 360of619 WIL 417002 HAP Task Force Page 8 A 90 DAY INHALATION TOXICITY STUDY OF 1,1,2 TRICHLOROETHANE (l, l, ZTCE) IN RATS (WITH SATELLITE GROUPS FOR PHARMACOKENETIC EVALUATIONS IN RATS AND MICE)

TABLE I 361 of 619 WIL 417002 HAPTaskForce A 90 DAY INHALATION TOXICITY STUDY OF 1,1,2 TRICHLOROETHANE (1,1,2 TCE) IN RATS {WITH SATELLITE GROUPS FOR PHARMACOKINETIC EVALUATIONS IN RATS AND MICE\

Table I: Purity Assessments Collected Analyzed ga& pa&

5116/ 01 6/ 6/ 01 Reference # Sample j417002 1 ID l 2 5015A# 2 Drum  beginning of use Run Number 5 Retention Time 6.10 6.69 9.44 10.8 13.3 13.5 14.1 15.2 15.9 16.0 19.0 22.3 23.0 25.4 25.6 25.8 Area Response 343 6.75 314 6.45 1.09 71388 0.755 0.956 1.68 1.80 26.7 3.10 4.09 8.10 2.81 3.44 total area 72112.7 Area @J 0.476 0.00937 0.436 0.00894 0.00151 99.00 0.00105 0.00133 0.00233 0.00249 0.0371 0.00430 0.00567 0.0112 0.00390 0.00477 Mean Area 3 98.98 WIL 417002 HAPTaskForce Collected pa&

5l16101 A 90 DAY INHALATION TOXICITY STUDY OF I, I, Z TRICHLOROETHANE (l, l, P TCE) IN RATS (WITH SATELLITE GROUPS FOR PHARMACOKINETIC EVALUATIONS IN RATS AND MICE\

Table I: Purity Assessments Analyzed Reference # Sample Run Retention Area Area p& (417002~\ E Number Time Response j% J 6/ 6/ 01 1 2 5015A# 2 6 6.10 343 0.483 Drum beginning of use 6.68 6.51 0.00915 9.44 313 0.441 10.8 6.28 0.00883 12.9 3.09 0.00435 13.3 0.989 0.00139 13.5 70357 98.97 14.1 0.845 0.00119 14.7 4.22 0.00594 15.2 0.822 0.00116 15.9 1.62 0.00280 16.0 1.69 0.00238 19.0 26.2 0.0369 20.3 1.24 0.00174 22.3 2.66 0.00375 23.0 3.79 0.00533 25.8 19.0 0.0268 total area 71092.0 Mean Area x WIL 417002 HAPTask Force Collected Date 9i21iOl A 90 DAY INHALATION TOXICITY STUDY OF 1 ,l, P TRICHLOROETHANE (1,1,2 TCE) IN RATS {WITH SATELLITE GROUPS FOR PHARMACOKINETIC EVALUATIONS IN RATS AND MICE)

Table I: Purity Assessments Analyzed Reference # Sample Run Retention &f& J417002 1 !! 2 Number Time 9127101 8 2 5015A# 2 13 6.54 Drum endofuse 6.87 9.52 10.1 Area Area Response (%) &

17.3 0.00652 99.30 1147 0.432 2.05 0.000770 881 0.332 11.6 23.2 0.00873 14.0 264630 99.22 15.2 1.08 0.000410 15.7 3.80 0.00143 15.9 1.93 0.000730 16.6 1.07 0.000400 16.9 1.66 0.000630 22.7 1.02 0.000380 total area 266710.6 Mean Area WIL 417002 HAPTaskForce A 90 DAY INHALATION TOXICITY STUDY OF 1,1,2 TRICHLOROETHANE (1,1,2 TCE) IN RATS JWITH SATELLITE GROUPS FOR PHARMACOKINETIC EVALUATIONS IN RATS AND MICE)

Table I: Purity Assessments Collected Analyzed Reference # Sample Run Retention Area pa& ga& /417002 1 i2 Number Time Response 9/ 21101 9/ 27/ 01 8 2 5015A# 2 14 6.58 7.950 Drum endofuse 6.92 1964 9.54 3.033 10.1 1482 11.8 52.01 14.0 580935 15.2 2.905 15.7 9.994 15.9 4.629 16.4 0.8537 16.5 0.9745 16.6 2.753 16.9 4.079 19.4 1.308 22.7 4.104 24.9 1.355 25.2 3.052 total area 584480 Area Mean Area fxJ z 0.00136 0.336 0.000520 0.254 0.00890 99.39 0.000500 0.00171 0.000790 0.000150 0.000170 0.000470 0.000700 0.000220 0.000700 0.000230 0.000520 Overall Mean (%) 99.14 WIL 4 17002 HAP Task Force 1, 1,2 Trichloroethane APPENDIX C Test Atmosphere Generation and Environmental Conditions During Exposure (WIL Research Laboratories, Inc.)

366 of 619 WIL 4 17002 HAP Task Force Inhalation Report Page 1 A 90 Day Inhalation Toxicity Study of 1,1,2 Trichloroethane (1,1,2 TCE) In Rats (with Satellite Groups for Pharmacokinetic Evaluations in Rats and Mice)

Table of Contents I. Exposure Methods A. Exposure Regimen B. Inhalation Exposure Systems Description II.