III. C. Animal Transport and Placement D. Exposure Environmental Conditions Test Atmosphere Generation Methods Methods of Characterization of Exposure Atmospheres A.

B.

C. Nominal Exposure Concentrations Actual Exposure Concentrations Determination of Homogeneity and Temporal Stability of Exposure Atmospheres D. Chemical Purity in Vapor Test Atmosphere IV. E. Aerosol Monitoring Results of Characterization of Exposure Atmospheres A. Nominal Exposure Concentrations B. Actual Exposure Concentrations 367 of 619  WIL 4 17002 HAP Task Force Inhalation Report Page 2 A 90 Day Inhalation Toxicity Study of 1,1,2 Trichloroethane (1,1,2 TCE) In Rats (with Satellite Grouns for Pharmacokinetic Evaluations in Rats and Mice)

Index of Figures 1. Schematic Diagram of Generation and Exposure System (CH 2 & 3)

2. Schematic Diagram of Generation and Exposure System (CH 4)

Index of Tables 1.

2.

3.

4.

5.

6.

7.

8. Daily Mean Chamber Temperature (Degrees Celsius)

Daily Mean Chamber Relative Humidity (%)

Daily Mean Chamber Ventilation Rate (SLPM)

Test Atmosphere Homogeneity Determination Daily Nominal Concentrations (ppm)

Daily Mean Test Atmosphere Concentrations (PPM)

Daily Environmental Conditions for Secondary Chamber 4 Daily Concentrations for Secondary Chamber 4 368 of 619 NIL 4 17002 HAP Task Force Inhalation Report Page 3 I. EXPOSURE METHODS A. Exposure Repimen The test article was administered as a daily six hour exposure.

Exposures were conducted five days per week at approximately the same time each day for 90 days (66 total exposure days). Animals in the control group were subjected to the identical exposure regimen.

B. Inhalation Exposure Svstems Description Except for pharmacokinetic group rats on their day of scheduled blood collection (see section II), exposures were conducted in four 2 m3 stainless steel and glass whole body inhalation exposure chambers (Hazleton H 2000). One chamber was dedicated for each group for the duration of the study. Chamber supply air was provided from a HEPA

and charcoal filtered, temperature and humidity controlled source.

Exhaust from the exposure system entered the main exhaust system, and was treated with a carbon scrubber prior to discharge from the laboratory.

C. Animal Transport and Placement Animals were individually housed in stainless steel wire mesh cage batteries suspended over cage board during the exposures. Food and water were not available for rats and food was not available for pharmacokinetic group mice during exposure period.

Animals were housed in a normal colony room during the non exposure hours. For each day's exposure, the animals were transferred to the appropriate exposure caging, transported to the exposure chamber room, exposed for the requisite duration and returned to their home cages.

The cage batteries were rotated on a daily basis through six battery positions in the chambers to help ensure a similar exposure for all animals within each group over the duration of the exposure period.

369 of 619 WIL 4 17002 HAP Task Force Inhalation Report Page 4 D. Exposure Environmental Conditions Temperature, relative humidity, chamber ventilation rate, and negative pressure within the chambers were monitored continuously and recorded manually approximately every 35 minutes.

Chamber temperature and relative humidity were monitored by 100 ohm platinum resistance temperature detectors and thin film polymeric sensors, respectively (Omega Engineering, Inc., Stamford, CT).

Airflow through the chamber was monitored by the pressure drop across a sharp edge orifice plate as measured by differential pressure gauges (Dwyer Magnehelic@ Indicating Transmitter, Michigan City, IN) and was set such that there were 12 15 air changes per hour. These gauges were calibrated under the conditions of exposure for conversion from pressure to airflow in liters per minute (LPM) through the use of a FM Series Axial Turbine Flow Transducer (Flowmetrics, Chatsworth, CA). Oxygen content was measured during the pre exposure method development phase with a MSA Remote Sampling Sensor (Pittsburgh, PA). Oxygen content was approximately 20.5%.

Daily means for chamber temperature, relative humidity, and chamber ventilation rate over the study period are presented in Tables 1 through 3 of this appendix, respectively. The following table summarizes the grand mean environmental conditions for all four study groups:

370 of 619 WIL 4 17002 HAP Task Force Inhalation Report Page 5 Temperature Mean (" C): Standard Deviation: (days) 1 Grow 2 Grow 3 Group 4 Group 21 22 22 25 0.8 0.7 0.8 0.9 66 66 66 66 Relative Humidity Mean (%): 49 46 44 42 Standard Deviation: 2.3 2.1 3.6 4.0 (days) 66 66 66 66 Chamber Ventilation Rate Mean (SLPM): Standard Deviation: (days) 434 440 4.4 12.2 66 66 421 433 11.3 4.9 66 66 II. TEST ATMOSPHERE GENERATION METHODS Vapor atmospheres of the test article were generated using a system similar to that shown in Figure 1 (CH 2 & 3) and Figure 2 (CH 4). The system operated as follows: test article was metered using a Harvard Compact Infusion pump (Harvard Apparatus, Hollison, MA) for CH 2 and 3, and a positive displacement pump (Fluid Metering, Inc., Oyster Bay, NY) for CH 4 at a known and constant rate to glass vaporization columns. The vaporization columns for test article exposure groups 2, 3, and 4 were 2.4 cm inner diameter (I. D.) by approximately 75 cm and were filled with 6, 8 and 12 n m 1 glass beads. The vaporization columns were wrapped with heating tapes that were controlled by temperature controllers (Model CN370, Omega Engineering, Stamford, CT).

Compressed air was metered to the bottom of each column using a rotameter

type flowmeter (Model No. FM 1050, Gilmont Instruments, Barrington, IL).

The liquid test article was delivered onto the heated glass beads and vaporized and the resulting vapors were carried out of the generators by the air flowing upward through the columns. The concentrated vapors in each system were piped to the chamber inlet where the concentration was diluted to the target concentration by the chamber ventilation airflow.

371 of 619 WIL 4 17002 HAP Task Force Inhalation Report Page 6 The following table defines the system conditions for each exposure chamber:

Chamber Number 1 2 3 4 Approximate Test Vaporization Vaporization Chamber Article Column Flow Rate of Ventilation Metering Pump Flow Rate Temp. Compressed Air Flow Rate Device Setting (m1imin. j li2.! L2 a? J3dJ cLE!& J NA NA NA 75 17 430 Harvard 20 ind. 0.038 75 18 430 Harvard 20 ind. 0.080 75 19 430 FM1 30 40% 0.186 0.242 75 17 430 (till scale)

A 500 L glass and stainless steel whole body inhalation chamber was used for pharmacokinetic study rats on the days the bloods were collected for Group 5 (100 ppm). The secondary chamber (CH4S) for Group 5 ran under the same conditions as Chambers 2 and 3 with the exception of the test article delivery on 6/ 25/ 01 and ventilation flow rate through the chamber. A Raze1 syringe pump (Raze1 Scientific Instruments, Inc., Stamford, CT) was used on 6/ 25/ 01 for the delivery of the test article to the bead column. The Raze1 syringe pump was removed following exposures on 6/ 25/ 01 and a Harvard syringe pump was used for test article delivery for the remainder of exposures conducted in this chamber.

The following table indicates the specific conditions for CH 4 S each day it was used for exposure:

Target Column Test Article Compressed Air Ventilation Exposure Concentration Temperature Flow Rate Flow Rate Flow Rate && 4iLlPdl (Des C] &nl/ min. J 4!? ilba (. L&. Q 0.1600 612510 1 100 75 0.282 13 400 6121101 100 75 0.092 13 150 612910 1 100 75 0.092 13 150 The use of a higher than normal ventilation rate for the 500 L chamber on 6/ 25/ 01 would not be expected to affect the exposure of the pharmacokinetic group rats on that day.

372 of 619 WIL 4 17002 HAP Task Force Inhalation Report Page 7 Oxygen content of the exposure atmospheres in CH 4 S was determined during the 6/ 29/ 01 animal exposure in the same manner as Chambers 2 4 with a calibrated oxygen sensor. The oxygen content was greater than 19%. Daily means for chamber temperature, relative humidity, and chamber ventilation rate for CH 4 S are presented in Table 7.

III. METHODS OF CHARACTERIZATION OF EXPOSURE ATMOSPHERES A. Nominal Exposure Concentrations A nominal exposure concentration was calculated for each daily exposure for each chamber from the total amount of test article used during the exposure and the total volume of air passed through the chamber during that day's exposure. The amount of test article used was obtained by weighing the reservoir containing the test article for each chamber prior to and after exposure each day. The total volume of air passed through each chamber was calculated from the daily average chamber ventilation flow rate in liters per minute (LPM) and the exposure duration.

The nominal concentration was calculated as follows:

ppm Trichloroethane =

Where: Wt. Trichloroethane . Mol. Vol. . lo6 MW . Ch. Flow * Exp. Dur.

Wt. Trichloroethane = Weight of test article in grams Mol. Vol. ZZ Molar volume at 730 m. mHg and 2 1° C 25.11 L/ mole 106 = ppm conversion factor = Trichloroethane molecular weight, 133.41 Ch. Flow z Daily average chamber flowrate for a given day, in LPM.

Exp. Dur. = Duration of a given day's exposure, in minutes 373 of 619 WIL 4 17002 HAP Task Force Inhalation Report Page 8 B. Actual Exposure Concentrations Actual exposure concentrations were determined using a Hewlett Packard 5890 Series II gas chromatograph (GC). Samples of the exposure atmospheres from each chamber were manually collected at approximate 35 minute intervals using a sample loop and multiposition valve.

The following table summarizes the GC conditions:

Instrument:

Detector:

Column:

Gases: Carrier  Helium Fuel  Hydrogen Air Temperatures (" C) Injector Column Detector Injection volume (ml)

Retention time (min.) Approximately 0.825 Integrator Run Parameters Chart Zero Offset Chart Attenuation Chart Speed Peak Area Rejection Value Peak Threshold Peak Width 0 1 1 .O cm/ min 0 2 0.04 Hewlett Packard 5890 Series II with a 3396A Series II integrator Flame ionization J & W DB Wax Narrowbore 30 m x 0.25 mm I. D., 1.5 micron film thickness Pressure (psinl Flow Rate (mVmin. 1 80 10 25 30 25 300 250 160, isothermal 250 0.25 The chromatograph was standardized using 40 liter Tedlar' gas bags prepared to contain known concentrations of the test article. The standard bags were prepared by injecting known volumes of test article into a 374 of 619 WIL 4 17002 HAP Task Force Inhalation Report Page 9 500 ml glass vaporization bulb heated to 80° C. A continuous flow of air carried the vaporized test article to a 40 liter bag. The total volume of air was measured by a dry test meter (Model DTM 200A, American Meter Co., Horsham, PA).

Concentrations of the gas phase standards were calculated as follows:

Concentration =

Where: VOL. R. T . D . 1O 3 1 lo6 L. GMW. P Concentration is in ppm VOL = volume of test article vaporized into bag in l. tl R = universal gas constant, 62.36 L mmHg mole K T = nominal laboratory temperature in K (273 + 21° C = 294 IS) D = 10" density of the test article, 1.4432 g/ ml = lo6 = ~1 to ml conversion factor conversion factor to ppm L = volume of air used to prepare bag, 30 liters GMW = gram molecular weight, 133.4 1 g/ mole P = nominal laboratory barometric pressure, 730 mmHg A calibration curve was prepared for concentration analysis during the study. The following table defines the standards prepared for this study:

Standard Concentration JrJ2? aL 9 27 72 109 136 Volume of Test Article Used 4L 1 3 8 12 15 Total Air Volume =e 30 30 30 30 Each standard was prepared in triplicate prior to the exposure period and analyzed with the GC. A least squares line was fitted to the resulting 375 of 619 WIL 4 17002 Page 10 HAP Task Force Inhalation Report peak areas. Concentrations were then calculated using the slope and intercept of the calibration curves.

On a daily basis, the integrity of the prime calibration curves was checked by analyzing one freshly prepared standard. On a rotational basis, a different one of the five standards was used each day. If the analyzed concentrations were within 110% of each known concentration,

C. the GC was considered within calibration specifications.

Determination of HomoPeneitv and Temporal Stab& v of Exposure Atmospheres Evaluation of the homogeneity of exposure concentrations was accomplished during the method development phase of the study prior to animal exposures. Four test locations and a reference location were used for these determinations. The test locations were Left Upper Front, Left Lower Rear, Right Upper Rear, Right Lower Front, identified as 1, 2, 3 and 4, respectively. The reference position was located in the approximate center of the chamber. Samples were collected as rapidly as possible, always collecting a sample from the reference location and then from one of the four test locations. For each test location, the measured concentration was calculated as a percent difference from the reference location. The homogeneity determination was performed in triplicate for each test article exposure. The results are presented in Table 4 of this appendix. The following table summarizes these data as the mean of the replicates for each chamber:

376of619 WIL 417002 HAP Task Force Inhalation Report Chamber Number 2 Test Mean % From Location Reference 1 5.6 2 1.7 3 3.0 4 4.0 3 1 2 3 4 5 4 1 2 3 4 0.8 0.1 0.8 0.0 0.0 0.7 0.0 0.3 4s 1 1.0 2 1.4 3 2.4 4 1.4 These data indicate that the homogeneity of each exposure atmosphere was adequate for the purpose of this study. To insure uniform exposure of all animals to the test article, animal cage batteries were rotated through the various possible chamber locations on a daily basis.

During the method development phase of this study the stability of the test article vapor concentrations over time was evaluated. Vapor chamber generated for six hours. Samples were taken approximately every 35 minutes and were found to be within the *lOoh range.

D. Chemical Puritv of the Test Atmosphere On 5/ 25/ 01 and 5/ 29/ 01 of method development, the chemical purity of the test atmosphere was analyzed by the following procedure. A sample of the test atmosphere was analyzed by the gas chromatograph.

The GC was optimized and the run length was extended to allow for detection of all test article associated peaks. The 109 ppm standard was 377 of 619 WIL 4 17002 HAP Task Force Inhalation Report then analyzed in the same manner. The percent of total peak area counts represented by the area counts for the atmosphere and standard peaks were calculated and compared. The 1,1,2 TCE peak represented 96.4%

and 96.3% of all peaks detected for the test atmosphere and 109 ppm standard, respectively. On 5/ 29/ 01 the 109 ppm standard and both chamber 4 test atmosphere samples were 100% of the 1,1,2 TCE peak.

E. Aerosol Monitoring Chamber 4 (100 ppm) was monitored for aerosol formation during the method development phase of the study. A light scattering aerosol photometer (MIE Industrial Dust Sensor, Model HPM 1000, MIE, Inc.,

Billerica, MA) was used on May 29, 2001 to monitor the chamber for aerosol formation. The monitor was connected to a strip chart recorder (Omega Engineering, Inc., Stamford, CT) such that a display of the dust sensor output was available to the operator. During actual determinations of aerosol formation in the chamber, aerosols were not detected at a level equal to or above the room and it was concluded that no aerosols were present.

IV. RESULTS OF CHARACTERIZATION OF EXPOSURE ATMOSPHERES A. Nominal Exnosure Concentrations The overall mean nominal concentrations were 24 ppm, 5 1 ppm and 128 ppm for the 15, 40 and 100 ppm groups, respectively. The daily nominal concentrations for CH l 4 are presented in Table 5 and for CH 4 S are presented in Table 8 of this appendix.

378 of 619 WIL 4 17002 HAP Task Force Inhalation Report Page B. Actual Exposure Concentrations The overal mean analyzed concentrations were I5 ppm, 40 ppm a 100 ppm for the 15, 40 and 100 ppm groups, respectively. Daily mean test atmosphere concentrations for CH 1 4 are presented in Table 6 and for CH 4 S 97 ppm overall mean concentration) are presented in Table 8 of this appendix.

prepar< ez Inhalation Toxicology Director, Inhalation Toxicology 379of619 WIL 4 17002 HAP Task Force Inhalation Report Page 14 Fim& 1. Schematic D& ram of Generation and Exposure System Chamber 2. a. nd 3 source of  Chamber Supply Air ZOOO Liter Hazleton Chamber ' To Compressed Air Source Column & Temperature Controller Orifice Meter +

To Chamber Exhaust 380 of 619 WIL 417002 HAP Task Force Inhalation Report Page 15 Fipe 2. Schematic Diagram of Genertition and Exposure System Chamber 4 Source of j ,i_ f3m~ b~; WP~ y ZOO& Liter Hazleton Chamber '

Orifice Meter i / /' To Compressed Air Source Column & Temperature / \

Controlfer Test Material To Chamber Exhaust 381 of 639 PROJECT NO.: WIL 4170021 SPONSOR: HAP TASK FORCE INHALATION TABLE 1 YO DAY INHAL. STUDY 1,1,2 TCE IN RATS WITH PK IN RATS & MICE DAILY MEAN CHAMBER TEMPERATURE (DEGREES CELSIUS)

____________~~ CHAMBER 1 CHAMBER 2 CHAMBER 3 CHAMBER 4 DATE GROUP: 0 PPM 15 PPM 40 PPM 100 PPM _____ 05/ 31/ 01 19. 19. 20. 22. 06/ 01/ 01 20. 20. 20. 23. 06/ 04/ 01 22. 23. 22. 24. 06/ 05/ 01 21. 22. 22. 25. 06/ 06/ 01 21. 22. 23. 25. 06/ 07/ 01 21. 22. 23. 26. 06/ 08/ 01 20. 21. 22. 26. 06/ 11/ 01 22. 22. 22. 26. 06/ 12/ 01 22. 23. 22. 26. 06/ 13/ 01 21. 22. 22. 26. 06/ 14/ 01 21. 22. 23. 25. 06/ 15/ 01 22. 22. 24. 26. 06/ 18/ 01 21. 22. 22. 27. 06/ 19/ 01 22. 22. 22. 26. 06/ 20/ 01 22. 24. 23. 25. 06/ 21/ 01 21. 22. 22. 25. % 06/ 22/ 01 21. 22. 23. 25. 06/ 25/ 01 21. 22. 23. 26. cn 06/ 26/ 01 21. 22. 22. 25. G 06/ 27/ 01 22. 22. 22. 26. 06/ 28/ 01 23. 23. 22. 25. 06/ 29/ 01 21. 22. 22. 24. 07/ 02/ 01 21. 22. 22. 24. 07/ 03/ 01 22. 22. 22. 24. 07/ 04/ 01 21. 21. 21. 24. 07/ 05/ 01 22. 22. 22. 24. 07/ 06/ 01 23. 23. 22. 24. PROJECT NO.: WIL 4170021 SPONSOR: HAP TASK FORCE INHALATION TABLE 1 90 DAY INHAL. STUDY 1,1,2 TCE IN RATS WITH PK IN RATS & MICE DAILY MEAN CHAMBER TEMPERATURE (DEGREES CELSIUS) PAGE 2 CHAMBER 1 CHAMBER 2 CHAMBER 3 CHAMBER 4 DATE GROUP : 0 PPM 15 PPM 40 PPM 100 PPM _________ 07/ 09/ 01 21. 22. 22. 24. 07/ 10/ 01 21. 22. 22. 24. 07/ 11/ 01 22. 22. 24. 25. 07/ 12/ 01 21. 22. 22. 24. 07/ 13/ 01 22. 22. 22. 24. 07j16jOl 23 07/ 17/ 01 21 07/ 18/ 01 21 07/ 19/ 01 22 07/ 20/ 01 21 07/ 23/ 01 22 07/ 24/ 01 23 07/ 25/ 01 21 07/ 26/ 01 21 07/ 27/ 01 22 07/ 30/ 01 21 % 07/ 31/ 01 22 08/ 01/ 01 23 CT\ 08/ 02/ 01 21 G OEjO3jOl 21 08/ 06/ 01 21 08/ 07/ 01 22 08/ 08/ 01 22 08/ 09/ 01 23 08/ 10/ 01 21 08/ 13/ 01 21 08/ 14/ 01 21 23. 22 24. 22. 22 24. 22. 23 24. 22. 23 25. 23. 22. 23. 22. 22. 22. 22. 22. 23. 23 22. 22 22 22 22 22 23 24 22 22 22. 23 22. 24 22. 22 22. 22 24. 23 22. 22 22. 23 22. 24 24. 24. 25. 24. 24. 26. 25. 25. 26. 25. 25. 26. 25. 25. 26. 25. 25. 26. . . . mulm NNN . . . NrnN NNN . . LnwLD NNN . . mmm NCUCU . . . . ln* II) uz NNNN 384of619 PROJECT NO.: WIL 4170021 SPONSOR: HAP TASK FORCE INHALATION TABLE 2 PO DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE DAILY MEAN CHAMBER RELATIVE HUMIDITY (%) PAGE 1 CHAMBER 1 CHAMBER 2 CHAMBER 3 CHAMBER 4 DATE GROUP: 0 PPM 15 PPM 40 PPM 100 PPM 05/ 31/ 01 06/ 01/ 01 06jO4jOl 06/ 05/ 01 06/ 06/ 01 06/ 07/ 01 06/ 08/ 01 06/ 11/ 01 06/ 12/ 01 06/ 13/ 01 06/ 14/ 01 06/ 15/ 01 06/ 18/ 01 06/ 19/ 01 06/ 20/ 01 06/ 21/ 01 0, 06/ 22/ 01 06/ 25/ 01 o\

5 06/ 26/ 01 06/ 27/ 01 06/ 28/ 01 06j29; Ol 07/ 02/ 01 07/ 03/ 01 07/ 04/ 01 07/ 05/ 01 07/ 06/ 01 _____________________ 50. 57. 51. 50. 51. 53. 54. 51. 51. 53. 48. 44. 52. 46. 47. 48. 48. 51. 49. 46. 50. 49. 47. 48. 48. 47. 46. 48. 54. 50. 46. 47. 49. 48. 50. 46. 47. 45. 49. 48. 44. 46. 45. 45. 48. 46. 44 46 46. 43. 47. 45. 45. 44. __________________ 47. 57. 48. 48. 49. 49. 48. 51. 50. 50. 46. 46. 52. 49. 48. 48. 42. 44. 44. 43. 44. 43. 40. 41. 43. 43. 40. 40. 46. 54. 48. 52. 57. 47. 48. 45. 45. 41. 51. 47. 44. 44. 42. 43. 47. 43. 44. 44. 42. 39. 42. 41. 40. 39. PROJECT NO.: WIL 4170021 SPONSOR: HAP TASK FORCE INHALATION TABLE 2 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE DAILY MEAN CHAMBER RELATIVE HUMIDITY (%) PAGE 2 ____________________________________~~~~~ CHAMBER 1 CAHMBER 2 CHAMBER 3 CHAMBER 4 DATE GROUP: 0 PPM 15 PPM 40 PPM 100 PPM __________________

07/ 09/ 01 50. 47. 44. 42. 07/ 10/ 01 50. 45. 42. 40. 07/ 11/ 01 49. 46. 40. 42. 07/ 12/ 01 50. 47. 44. 42. 07; 13jo1 46. 44. 43. 40: 07/ 16/ 01 49. 47. 44. 44. 07/ 17/ 01 50. 07/ 18/ 01 50. 07/ 19/ 01 49. 07; 20; 01 07/ 23/ 01 07/ 24/ 01 07/ 25/ 01 w 07; 26jOl % 07/ 27/ 01 07/ 30/ 01 % 07/ 31/ 01 08/ 01/ 01 CJY G 08/ 02/ 01 08/ 03/ 01 08/ 06/ 01 08/ 07/ 01 48. 48. 55. 50. 47. 46. 52. 47. 49. 49. 50. 49. 52. 47. 46. 46. 44. 45. 52. 46. 42. 44. 47. 44. 44. 45. 45. 46. 47. 44. 42. 41. 41. 44. 49. 43. 39. 40. 44. 42. 42. 42. 40. 40. 45. 42 41 41 40 41 48 42 38 39 41 40 40 39 40 38 45 08; 08; 01 48. 45. 44. 41. 08/ 09/ 01 52. 49. 47. 44. 08/ 10/ 01 47. 43. 41. 38. 08/ 13/ 01 48. 44. 41. 38. 08/ 14/ 01 48. 44. 40. 37. m I I , INHALATION TABLE 3 PROJECT NO.: WIL 4170021 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK RATS & MICE SPONSOR: HAP TASK FORCE DAILY MEAN CHAMBER VENTILATION RATE (SLPM) PAGE 1 CHAMBER 1 CHAMBER 2 CHAMBER 3 CHAMBER 4 DATE GROUP: 0 PPM 15 PPM 40 PPM 100 PPM ___________________________________ 05/ 31/ 01 06/ 01/ 01 06/ 04/ 01 06jO5jOl 06/ 06/ 01 06/ 07/ 01 06/ 08/ 01 06; ll; Ol 06/ 12/ 01 06/ 13/ 01 06/ 14/ 01 06/ 15/ 01 06/ 18/ 01 E 06/ 19/ 01 06/ 20/ 01 00 06/ 21/ 01 0 06/ 22/ 01 r 06/ 25/ 01 cn G 06/ 26/ 01 06/ 27/ 01 06/ 28/ 01 _ 433 433 433 433 433 429 429 434 434 437 437 438 433 433 434 445 437 441 421. 447. 432 446 426. 437. 433 451 416. 436. 433 437 424. 434. 436 441 422. 434. 429 424 438 440 424 437 425 421 461 451 457 450 431 454 450 451 ._______________________ 418. 418. 417. 415. 421. 419. 413. 412. 422. 418. 417. 426. 422. 430. 431. 427. .___________ 422. 431. 429. 433. 431. 431. 424. 431. 434. 439. 437. 436. 440. 434. 428. 437.

06j29jOl 435. 408. 419. 443. 07/ 02/ 01 439. 440. 425. 430. 07/ 03/ 01 441. 444. 426. 437. 07/ 04/ 01 430. 449. 424. 435. 07jO5jOl 437. 454. 419. 431. 07/ 06/ 01 438. 425. 429. 432. PROJECT NO.: WIL 4170021 SPONSOR: HAP TASK FORCE INHALATION TABLE 3 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK RATS & MICE DAILY MEAN CHAMBER VENTILATION RATE (SLPM) PAGE 2 _____________________________________ CHAMBER 1 CHAMBER 2 CHAMBER 3 CHAMBER 4 DATE GROUP: 0 PPM 15 PPM 40 PPM 100 PPM _________________________________

07/ 09/ 01 433. 450. 423.

07/ 10/ 01 431. 433. 458. 429. 07/ 11/ 01 434. 433. 453. 419. 07/ 12/ 01 439. 427. 450. 426.

07/ 13/ 01 433. 433. 441. 420. 07/ 16/ 01 429. 436. 456. 422. 07/ 17/ 01 428. 434. 45% 421. 422. 07/ 18/ 01 07/ 19/ 01 07/ 20/ 01 07/ 23/ 01 07/ 24/ 01 07/ 25/ 01 433. 436. 433. 434. 426. 428. 429. 430. 430.

E 07j26jOl 07/ 27/ 01 a 07/ 30/ 01 435. 440. 422 426 421 430 423 440 432 % 07/ 31/ 01 08/ 01/ 01 m z 08/ 02/ 01 08/ 03/ 01 08/ 06/ 01 08/ 07/ 01 432. 433. 430. 438. 433. 431 429 431. 430. 429. 430. 427. 436. 436.

08; 08; 01 08/ 09/ 01 08/ 10/ 01 08/ 13/ 01 08/ 14/ 01 432. 439. 434. 433. 431. 432. 431. 439. 444. 443. 454. 465. 436. 438. 433. 426. 437. 443. 438. 458. 440. 432. 448. 433. 421 409 428 431 421 421 430 420 415 430. 435. 432. 429. 432. 439. 431. 428. 433. 432. 432. 434. 449. 412 396 PROJECT NO.: WIL 4170021 SPONSOR: HAP TASK FORCE INHALATION TABLE 3 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK RATS & MICE DAILY MEAN CHAMBER VENTILATION RATE (SLPM) PAGE 3 CHAMBER 1 CHAMBER 2 CHAMBER 3 CHAMBER 4 DATE GROUP: 0 PPM 15 PPM 40 PPM 100 PPM ________________________________ 08/ 15/ 01 418. 460. 441. 08/ 16/ 01 436. 441. 436. 401. 08/ 17/ 01 427. 424. 441. 418. 441 08/ 20/ 01 425. 432. 413. 08/ 21/ 01 436. 441. 421. 402. 08/ 22/ 01 443. 438. 431. 409. 440. 08/ 23/ 01 437. 425. 410. 08/ 24/ 01 440. 437. 438. 422. 08/ 27/ 01 438. 434. 425. 404. 08/ 28/ 01 434. 441. 406. 388. 434. 08/ 29/ 01 441. 441. 408. 08/ 30/ 01 432. 433. 434. 386. 433. ____________________________________ GRAND MEAN 434. 440. 421. S. D. 4.4 12.2 11.3 C. V. (%) 1.01 2.78 2.68 433. 4.9 1.14 % N 66 66 66 66 6\ MANUALv1.00 F; 07/ 09/ 2001 R: 02/ 14/ 2002 PROJECT NO.: WIL 4170021 SPONSOR: HAP TASK FORCE INHALATION TABLE 4 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK RATS & MICE TEST ATMOSPHERE HOMOGENEITY DETERMINATION PAGE 1 ______________________________~~~~~~~ TARGET: 15 PPM CHAMBER #2 2.0 m3 ___________________________________~~~~ DATE SAMPLING LOCATION CONC. (PPM) % FROM REFERENCE ______________________________________~~~~~~~~~~~~ 05/ 27/ 01 REFERENCE PORT 16.1 LEFT UPPER FRONT 14.9 7.5 REFERENCE PORT 16.4 LEFT LOWER REAR 15.9 3.0 REFERENCE PORT 15.7 RIGHT UPPER REAR 17.4 10.8 REFERENCE PORT 15.6 RIGHT LOWER FRONT 14.9 4.5 05/ 27/ 01 REFERENCE PORT 16.0 LEFT UPPER FRONT 15.3 4.4 REFERENCE PORT 15.4 % LEFT LOWER REAR REFERENCE 15.1 PORT 15.9 1.9 E RIGHT UPPER REAR REFERENCE 14.9 PORT 16.6 6.3 RIGHT LOWER FRONT 15.9 4.2 o\ 5; 05/ 27/ 01 REFERENCE PORT 16.1 LEFT UPPER FRONT 15.3 5.0 REFERENCE PORT 15.7 LEFT LOWER REAR 15.7 0.0 REFERENCE PORT 15.7 RIGHT UPPER REAR 16.4 4.5 REFERENCE PORT 15.2 RIGHT LOWER FRONT 14.7 3.3 MEAN % FOR EACH LOCATION: LEFT UPPER FRONT 5.6 LEFT LOWER REAR 1.7 RIGHT UPPER REAR 3.0 RIGHT LOWER FRONT 4.0 ____________________________________~~~~~~ CONC. = CONCENTRATION PPM = PARTS PER MILLION PROJECT NO.: WIL 4170021 SPONSOR: HAP TASK FORCE INHALATION TABLE 4 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK RATS & MICE TEST ATMOSPHERE HOMOGENEITY DETERMINATION PAGE 2 TARGET: 40 PPM CHAMBER #3 2.0 m3 _________________________________~ DATE SAMPLING LOCATION CONC.( PPM) % FROM REFERENCE ____________________________________~ 05/ 29/ 01 REFERENCE PORT 39 LEFT UPPER FRONT 39 0.0 REFERENCE PORT 41 LEFT LOWER REAR 40 2.4 REFERENCE PORT 40 RIGHT UPPER REAR 39 2.5 REFERENCE RIGHT LOWER FRONT 38 0 05/ 29/ 01 REFERENCE LEFT UPPER FRONT 39 REFERENCE LEFT LOWER REAR 39 REFERENCE RIGHT UPPER REAR 40 REFERENCE RIGHT LOWER FRONT 40 PORT 39 PORT 39 0.0 PORT 40 PORT 40 0.0 PORT 39 2.6 05/ 29/ 01 REFERENCE PORT 40 LEFT UPPER FRONT 39 2.5 REFERENCE PORT 38 LEFT LOWER REAR 40 REFERENCE PORT 39 RIGHT UPPER REAR 39 0.0 REFERENCE PORT 40 RIGHT LOWER FRONT 40 0.0 MEAN % FOR EACH LOCATION: 2.6 2.5 5.3 LEFT UPPER FRONT 0.8 LEFT LOWER REAR 0.1 RIGHT UPPER REAR 0.8 RIGHT LOWER FRONT 0.0 _____________________________________ CONC. = CONCENTRATION PPM = PARTS PER MILLION PROJECT NO.: WIL 4170021 SPONSOR: HAP TASK FORCE INHALATION TABLE 4 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK RATS & MICE TEST ATMOSPHERE HOMOGENEITY DETERMINATION PAGE 3 __________~~ TARGET: 100 PPM CHAMBER #4 2.0 m3 ____________~~ DATE SAMPLING LOCATION CONC. (PPM) % FROM REFERENCE ______________________________~~~~~

05/ 26/ 01 REFERENCE PORT 101 LEFT UPPER FRONT 101 0.0 REFERENCE PORT 102 LEFT LOWER REAR 101 1.0 REFERENCE PORT 101 RIGHT UPPER REAR 102 1.0 REFERENCE PORT 100 RIGHT LOWER FRONT 101 1.0 05/ 26/ 01 REFERENCE PORT 101 LEFT UPPER FRONT 103 2.0 REFERENCE PORT 102 LEFT LOWER REAR 101 REFERENCE PORT 102 RIGHT UPPER REAR 101 1.0 REFERENCE PORT 101 % RIGHT LOWER FRONT 101 0.0 a\

t; 05/ 26/ 01 REFERENCE PORT 102 LEFT UPPER FRONT 100 2.0 REFERENCE PORT 101 LEFT LOWER REAR 101 REFERENCE PORT 101 RIGHT UPPER REAR 101 0.0 REFERENCE PORT 101 RIGHT LOWER FRONT 101 0.0 1.0 0.0 MEAN % FOR EACH LOCATION: LEFT UPPER FRONT 0.0 LEFT LOWER REAR 0.7 RIGHT UPPER REAR 0.0 RIGHT LOWER FRONT 0.3 ___________________________________~~~~~~~~ CONC. = CONCENTRATION PPM = PARTS PER MILLION PROJECT NO.: WIL 4170021 SPONSOR: HAP TASK FORCE INHALATION TABLE 4 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK RATS & MICE TEST ATMOSPHERE HOMOGENEITY DETERMINATION PAGE 4 _____________________________~~ TARGET: 100 PPM CHAMBER #4 S 0.5 m3 ______________________________~ DATE SAMPLING LOCATION CONC. (PPM) % FROM REFERENCE ____________________________________~~~~~~~~~~ 06/ 28/ 01 REFERENCE PORT 94 LEFT UPPER FRONT 94 0.0 REFERENCE PORT 98 LEFT LOWER REAR 96 2.0 REFERENCE PORT 97 RIGHT UPPER REAR 96 1.0 REFERENCE PORT 96 RIGHT LOWER FRONT 95 1.0 06/ 28/ 01 REFERENCE PORT 97 LEFT UPPER FRONT 96 1.0 REFERENCE PORT 97 LEFT LOWER REAR 97 0.0 REFERENCE PORT 98 RIGHT UPPER REAR 96 2.0 REFERENCE PORT 98 RIGHT LOWER FRONT 97 1.0 06/ 28/ 01 REFERENCE PORT 98 LEFT UPPER FRONT 96 2.0 REFERENCE PORT 98 LEFT LOWER REAR 96 2.0 REFERENCE PORT 98 RIGHT UPPER REAR 94 4.1 REFERENCE PORT 98 RIGHT LOWER FRONT 96 2.0 MEAN % FOR EACH LOCATION: LEFT UPPER FRONT 1.0 LEFT LOWER REAR 1.4 RIGHT UPPER REAR 2.4 RIGHT LOWER FRONT 1.4 ________________________________ CONC. = CONCENTRATION PPM = PARTS PER MILLION MANUAL V1.00 07/ 17/ 2001 PROJECT NO.: WIL 4170021 SPONSOR: HAP TASK FORCE INHALATION TABLE 5 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE DAILY NOMINAL CONCENTRATION (PMM) PAGE 1 _____________________________________~ CHAMBER 1 CHAMBER 2 CHAMBER 3 CHAMBER 4 DATE GROUP: 0 PPM 15 PPM 40 PPM 100 PPM ______________________________________ 05/ 31/ 01 0. 25. 51. 123. 06/ 01/ 01 0. 24. 51. 130. 06/ 04/ 01 0. 24. 51. 127. 06/ 05/ 01 0. 24. 52. 128. 06/ 06/ 01 0. 25. 51. 126. 06/ 07/ 01 0. 24. 50. 127. 06; 08jol 0. 26 52. 125. 06/ 11/ 01 0. 25 52. 131. 06/ 12/ 01 06/ 13/ 01 06; 14; 01 06/ 15/ 01 06/ 18/ 01 06/ 19/ 01 06/ 20/ 01 06/ 21/ 01 0 06/ 22/ 01 2 06/ 25/ 01 z 06/ 26/ 01 06/ 27/ 01 06/ 28/ 01 06/ 29/ 01 07/ 02/ 01 07/ 03/ 01 07/ 04/ 01 07/ 05/ 01 07/ 06/ 01 0. 23 0. 23 0. 24 0. 23 0. 22 0. 23 0. 23 0. 25 0. 24 0. 24 0. 23 0. 25 0. 24 0. 26 0. 24 0. 24 0. 23 0. 24 0. 24 51. 51. 52. 49. 50. 49. 50. 50. 51. 50. 51. 50. 50. 51. 50. 50. 51. 51. 50. 129. 128. 130. 125. 129. 126. 128. 130. 128. 127. 128. 128. 128. 127. 128. 123. 128. 129. 128. PROJECT NO.: WIL 4170021 SPONSOR: HAP TASK FORCE INHALATION TABLE 5 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE DAILY NOMINAL CONCENTRATION (PPM) PAGE 2 CHAMBER 1 CHAMBER 2 CHAMBER 3 CHAMBER 4 DATE GROUP: 0 PPM 15 PPM 40 PPM 100 PPM __________________________________~~~~~~~~~~~ 07/ 09/ 01 0. 23. 50. 129. 07/ 10/ 01 0. 23. 50. 129. 07/ 11/ 01 0. 23. 52. 127. 07/ 12/ 01 0. 23. 50. 129. 07/ 13/ 01 0. 24. 50. 127. 07/ 16/ 01 0. 23. 51. 127. 07/ 17/ 01 0. 23. 51. 129. 07/ 18/ 01 0. 24. 50. 128. 07/ 19/ 01 0. 24. 50. 123. 07/ 20/ 01 0. 23. 51. 128. 07/ 23/ 01 0. 23. 49. 127. 07/ 24/ 01 0. 22. 50. 127. 07/ 25/ 01 0. 24. 48. 127. 07/ 26/ 01 0. 24. 49. 127. 07/ 27/ 01 0. 24. 49. 125. 07/ 30/ 01 0. 24. 51. 125. 07/ 31/ 01 0. 24. 51. 127. 08/ 01/ 01 0. 24. 48. 128. 08/ 02/ 01 0. 24. 50. 128. 08/ 03/ 01 0. 24. 49. 129. 08/ 06/ 01 0. 24. 49. 127. 08/ 07/ 01 0. 24. 51. 128. 08/ 08/ 01 0. 23. 52. 128. 08/ 09/ 01 0. 25. 51. 127. 08/ 10/ 01 0. 25. 51. 126. 08/ 13/ 01 0. 24. 51. 128. 08/ 14/ 01 0. 24. 55. 128. zooz/ PK/ zo:~ TOOZ/ 6O/ LO 0 0 . t*? urlNuw 99 P'K 28. K .8ZK ______ E'P 0 (2) 'A'3 _ '" 1I_..~~~~~ z 'PZ '0 mi3w am3 m __ '82 i 'EZK '8ZK . LZK . Of E '8ZK 'EEK '9ZK "KEK .9ZK 'TET: `LZTm __________________________________ 99 f'fi OZ'Z 'KS ._____

'SS 'ZS . f9 . BS 'KS 'ZS . zs . ES . ES . tS '9s .9s . PZ '0 i O/ Of/ 80 'PZ '0 TO/ 6Z/ 80 'LZ '0 10/ 82/ 80 'LZ '0 CO/ LZ/ 80 'LZ '0 TO/ tZ,` EO 'SZ '0 IO/ EZ/ 80 'PZ '0 TO/ ZZ/ 80 'SZ '0 TO/ TZ/ 80 sz '0 CO/ OZ/ 80 . tiz '0 IO/ LI/ 80 'PZ '0 KO/ 9K/ 80 'EZ '0 TO/ ST/ 80 lzldd 001 Wdd OP Wdd 5T PJdd 0 :dClOtIE) 3ma f/ tI38bwH3 f ?lIB'FlWVHS Z ?338KVH3 5 tI38blUH3 _____________________________________

(Iddd) NOIJkPLLN33N03 ?IVNIblNON XlIYcI f 33Vd 3SIW 3 SLLtltI NI Xd HLIM SJiVtl NI 3X Z'T'T: d0 ~aI LT. S "IVHNI AVa 06 S B? S. 'rld NOI, LV? VHNI 33'dOJ HSIL dYH: XOSNOdS IZOOLIti TIM: 'ON 633POtId PROJECT NO.: WIL 4170021 SPONSOR: HAP TASK FORCE INHALATION TABLE 6 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE DAILY MEAN TEST ATMOSPHERE CONCENTRATIONS PAGE 1 GROUP : 0 RPM 15 PPM 40 PPM 100 RPM ______________________________________

CH. NO. 1 2. OM3 CH. NO. 2 2. OM3 CH. NO. 3 2. OM3 CH. NO. 4 2. OM3 CONC . CONC . CONC . CONC . ______________________________________~

EXPOSURE ON 31 MAY 01 MEAN 0. 15. 40. 96. E(%, 0.00 0.0 4.16 0.6 2.71 1.1 2.45 2.4 N 10 10 10 10 EXPOSURE ON Ol JUN 01 MEAN 0. 15. SD 0.0 0.7 CV(%) 0.00 4.89 N 10 10 EXPOSURE ON 04 JUN 01 MEAN 0. 15. SD 0.0 0.7 CV(%) 0.00 4.41 N 10 10 EXPOSURE ON 05 JUN 01 MEAN 0. 15. SD 0.0 0.8 CV(%) 0.00 5.44 N 10 10 EXPOSURE ON 06 JUN 01 MEAN 0. 15. SD 0.0 1.3 CV(%) 0.00 8.52 N 10 10 40. 0.9 2.39 10 40. 0.7 1.77 10 40. 100. 0.7 3.9 1.77 3.96 10 10 40. 100. 1.4 2.5 3.61 2.55 10 10 102. 3.3 3.22 10 101. 3.3 3.23 10 CH. = CHAMBER, NO. = NUMBER, CONC. = CONCENTRATION CONCENTRATION IN: PPM PROJECT NO.: WIL 4170021 SPONSOR: HAP TASK FORCE INHALATION TABLE 6 PO DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE DAILY MEAN TEST ATMOSPHERE CONCENTRATIONS PAGE 2 GROUP: 0 PPM 15 PPM 40 PPM 100 PPM CH. NO. 1 2. OM3 CH. NO. 2 2. OM3 CH. NO. 3 2. OM3 CH. NO. 4 2. OM3 CONC . CONC . CONC . CONC .

EXPOSURE ON 07 JUNO1 MEAN %%, N EXPOSURE ON 08 JUNO1 MEAN SD CV(%) N EXPOSURE ON ll JUN 01 MEAN SD CV(%) g N o\

5, EXPOSURE ON 12 JUN 01 MEAN EXPOSURE ON MEAN E(%, N 0. 15. 39. 100. 0.0 0.4 1.1 1.3 0.00 2.77 2.13 1.31 10 10 10 10 0. 14. 39. 97. 0.0 0.8 0.9 7.5 0.00 5.76 2.42 7.74 10 10 10 10 0. 15. 40. 100. 0.0 1.0 0.9 4.5 0.00 6.98 2.39 4.54 10 10 10 10 0. 16. 40. 100. 0.0 0.5 1.1 2.8 0.00 3.40 2.76 2.84 10 10 10 10 13 JUNO1 0. 16. 40. 100. 0.0 1.1 0.9 1.8 0.00 6.89 2.19 1.81 10 10 10 10 CH. = CHAMBER, NO. = NUMBER, CONC. = CONCENTRATION CONCENTRATION IN: PPM PROJECT NO.: WIL 4170021 SPONSOR: HAP TASK FORCE INHALATION TABLE 6 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE DAILY MEAN TEST ATMOSPHERE CONCENTRATIONS PAGE 3 _______________________________________ GROUP: 0 PPM 15 PPM 40 PPM 100 PPM ______________________________________ CH. NO. 1 2. OM3 CH. NO. 2 2. OM3 CH. NO. 3 2. OM3 CH. NO. 4 2. OM3 CONC . CONC . CONC CONC . ______________________________________

EXPOSURE ON 14 JUN 01 MEAN 0. 15. 39. 100. %(%) 0.00 0.0 3.14 0.5 4.96 2.0 4.31 4.3 N 10 10 10 10 EXPOSURE ON 15 JUN 01 MEAN SD CV(%) N 0. 15. 40. 99. 0.0 0.6 0.8 3.7 0.00 3.76 2.04 3.81 10 10 10 10 EXPOSURE ON 18 JUN 01 MEAN SD CV(%) N 0. 15. 40. 101. 0.0 1.2 1.0 2.1 0.00 8.09 2.48 2.05 10 10 10 10 EXPOSURE ON 19 JUN 01 MEAN 0. 15. SD 0.0 0.8 CV(%) 0.00 5.19 N 10 10 EXPOSURE ON 20 JUN 01 MEAN 0.

E(%, 0.0 0.00 N 10 CH. = CHAMBER, NO. = NUMBER, CONC. = CONCENTRATION CONCENTRATION IN: PPM 16. 0.5 3.40 10 40. 99. 1.1 3.0 2.85 3.00 10 10 40. 1.3 3.37 10 100. 5.0 5.01 10 IlVxw. lH. L. L" I" IHBllI! a 0 PROJECT NO.: WIL 4170021 PO DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 4 SPONSOR: HAP TASK FORCE DAILY MEAN TEST ATMOSPHERE CONCENTRATIONS ___________~~~ GROUP: 0 PPM 15 PPM 40 PPM 100 PPM ______________~~~ CH. NO. 1 2. OM3 CH. NO. 2 2. OM3 CH. NO. 3 2. OM3 CH. NO. 4 2. OM3 CONC . CONC . CONC . CONC . ____________________________________

EXPOSURE ON 21 JUN 01 MEAN SD cvt%) N 0. 16. 40. 102. 0.0 1.8 1.0 2.7 0.00 11.39 2.48 2.63 10 10 10 10 EXPOSURE ON 22 JUN 01 MEAN 0. E(%, 0.00 0.0 N 10 EXPOSURE ON 25 JUN 01 MEAN SD CV(%) N 0. 0.0 0.00 10 EXPOSURE ON 26 JUN 01 MEAN 0. SD 0.0 CV(%) 0.00 N 10 EXPOSURE ON 27 JUN 01 MEAN 0. SD 0.0 CV(%) 0.00 N 10 CH. = CHAMBER, NO. = NUMBER, CONC. = CONCENTRATION CONCENTRATION IN: PPM 16. 39. 101. 1.0 4.0 1.6 6.27 10.12 1.56 10 10 10 15. 39. 100. 0.8 0.8 5.7 5.19 2.14 5.71 10 10 10 15. 40. 100. 0.8 0.9 0.9 5.38 2.29 0.92 10 10 10 15. 40. 100. 0.7 0.7 0.7 4.95 1.67 0.67 10 10 10 INHALATION TABLE 6 PROJECT NO.: WIL 4170021 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 5 SPONSOR: HAP TASK FORCE DAILY MEAN TEST ATMOSPHERE CONCENTRATIONS _____________~ GROUP: 0 PPM 15 PPM 40 PPM 100 PPM CH. NO. 1 2. OM3 CH. NO. 2 2. OM3 CH. NO. 3 2. OM3 CH. NO. 4 2. OM3 CONC . CONC . CONC . CONC .

EXPOSURE ON 28 JUNO1 MEAN 0. 15. 40. 101. SD 0.0 1.0 1.1 2.0 CV(%) 0.00 6.79 2.63 1.97 N 10 10 10 10 EXPOSURE ON 29 JUN 01 MEAN 0. SD 0.0 CV(%) 0.00 N 10 EXPOSURE ON OZ JUL 01 ' SD MEAN td 0 CV(%) `+ a N o\

5; EXPOSURE ON 03 JUL 01 MEAN 0. 15. 39. SD 0.0 1.7 0.8 CV(%) 0.00 11.02 2.14 N 10 10 10 EXPOSURE ON 04 JUL 01 MEAN 0. 15. 40. E(%, 0.00 0.0 8.04 1.2 1.59 0.6 N 10 10 10 0. 0.0 0.00 10 15. 40. 100. 3.4 1.1 1.4 23.14 2.85 1.42 10 10 10 16. 40. 100. 1.2 1.2 1.5 7.60 3.12 1.48 10 10 10 CH. = CHAMBER, NO. = NUMBER, CONC. = CONCENTRATION CONCENTRATION IN: PPM PP. 2.9 2.89 10 100. 1.2 1.23 10 PROJECT NO.: WIL 4170021 SPONSOR: HAP TASK FORCE INHALATION TABLE 6 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE DAILY MEAN TEST ATMOSPHERE CONCENTRATIONS PAGE 6 GROUP: 0 PPM 15 PPM 40 PPM 100 PPM _____________________________________~~~~~ CH. NO. 1 2. OM3 CH. NO. 2 2. OM3 CH. NO. 3 2. OM3 CH. NO. 4 2. OM3 CONC . CONC . CONC . CONC . _____________^______________________~~~~~~~~~

EXPOSURE ON 05 JUL 01 MEAN 0. 15. 40. SD 0.0 0.7 1.2 CV(%) 0.00 4.41 3.09 N 10 10 10 EXPOSURE ON 06 JUL 01 MEAN 0. 15. 40. SD 0.0 2.0 1.3 CV(%) 0.00 13.33 3.31 N 10 10 10 EXPOSURE ON 09 JUL 01 MEAN 0. 15. %%) 0.00 0.0 3.16 0.5 N 10 10 EXPOSURE ON lo JUL 01 MEAN 0. 15. SD 0.0 0.6 CV(%) 0.00 4.16 N 10 10 EXPOSURE ON ll JUL 01 MEAN 0. 15. SD 0.0 0.8 CV(%) 0.00 5.19 N 10 10 CH. = CHAMBER, NO. = NUMBER, CONC. = CONCENTRATION CONCENTRATION IN: PPM 40. 1.0 2.48 10 40. 1.0 2.49 10 40. 100. 0.7 0.3 1.70 0.32 10 10 100. 0.4 0.42 10 100. 0.9 0.92 10 100. 0.8 0.82 10 100. 0.9 0.87 10 PROJECT NO.: WTL 4170021 SPONSOR: HAP TASK FORCE INHALATION TABLE 6 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE DAILY MEAN TEST ATMOSPHERE CONCENTRATIONS PAGE I ______________________________________ GROUP: 0 PPM 15 PPM 40 RPM 100 PPM ________________________________________

CH. NO. 1 2. OM3 CH. NO. 2 2. OM3 CH. NO. 3 2. OM3 CH. NO. 4 2. OM3 CONC . CONC CONC . CONC ____________________________________

EXPOSURE ON 12 JUL 01 MEAN 0. 15. 40. 100. SD 0.0 1.0 1.4 1.0 CV(%) 0.00 6.59 3.61 0.96 N 10 10 10 10 EXPOSURE ON 13 JUL 01 MEAN SD CV(%) N EXPOSURE ON 16 JUL 01 MEAN E(%, g N m ;s; EXPOSURE ON 17 JUL 01 MEAN SD CV(%) N EXPOSURE ON 18 JUL 01 MEAN SD CV(%) N CH. = CHAMBER, NO. = NUMBER, CONCENTRATION IN: PPM 0. 0.0 0.00 10 0. 15. 40. 100. 0.0 0.8 0.7 0.5 0.00 5.44 1.70 0.48 10 10 10 10 0. 15. 40. 100. 0.0 0.9 0.9 0.6 0.00 5.88 2.39 0.57 10 10 10 10 0. 15. 40. 100. 0.0 1.1 1.0 1.9 0.00 7.61 2.48 1.95 10 10 10 10 CONC. = CONCENTRATION 16. 1.1 6.97 10 40. 100. 1.3 1.3 3.21 1.32 10 10 PROJECT NO.: WIL 4170021 INHALATION TABLE 6 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 8 SPONSOR: HAP TASK FORCE GROUP: 0 PPM CH. NO. 1 2. OM3 DAILY MEAN TEST ATMOSPHERE CONCENTRATIONS 15 PPM 40 PPM CH. NO. 2 2. OM3 CH. NO. 3 2. OM3 ___ CONC . CONC . _________________~~~~~~~ 100 PPM CH. NO. 4 2. OM3 CONC . CONC .

EXPOSURE ON 19 JUL 01 MEAN SD CV(%) N EXPOSURE ON 20 JUL 01 MEAN SD CV(%) N EXPOSURE ON 23 JUL 01 $ SD MEAN CV(%) g N m G EXPOSURE ON 24 JUL 01 MEAN SD CV(%) N 0. 0.0 0.00 10 0. 15. 40. 100. 0.0 0.9 0.7 0.9 0.00 5.80 1.67 0.92 10 10 10 10 0. 16. 40. 100. 0.0 2.3 1.4 1.5 0.00 14.36 3.54 1.49 10 10 10 10 0. 17. 40. 100. 0.0 4.2 1.1 0.9 0.00 25.11 2.82 0.87 10 10 10 10 15. 0.6 3.76 10 EXPOSURE ON 25 JUL 01 MEAN 0. 14. SD 0.0 1.4 CV(%) 0.00 9.92 N 10 10 CH. = CHAMBER, NO. = NUMBER, CONC. = CONCENTRATION CONCENTRATION IN: PPM 40. 100. 1.0 1.0 2.59 1.00 10 10 40. 100. 2.5 1.1 6.32 1.06 10 10 PROJECT NO.: WIL 4170021 SPONSOR: HAP TASK FORCE INHALATION TABLE 6 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE DAILY MEAN TEST ATMOSPHERE CONCENTRATIONS PAGE 9 __________________________________ GROUP: 0 PPM 15 PPM 40 PPM 100 PPM _____________________________________ CH. NO. 1 2. OM3 CH. NO. 2 2. OM3 CH. NO. 3 2. OM3 CH. NO. 4 2. OM3 CONC . CONC . CONC . CONC _____________________________________~

EXPOSURE ON 26 JUL 01 MEAN 0. 15. 40. 100. SD 0.0 1.7 1.0 1.6 CV(%) 0.00 11.83 2.48 1.57 N 10 10 10 10 EXPOSURE ON 27 JUL 01 MEAN 0. 15. 40. 99. SD 0.0 1.0 1.4 2.1 CV(%) 0.00 6.79 3.48 2.16 N 8 10 10 10 EXPOSURE ON 30 JUL 01 $i SD MEAN on, CV(%) N m z EXPOSURE ON 31 JUL 01 MEAN 0. 16. 40. 100. 0.0 1.8 0.9 1.5 0.00 11.39 2.29 1.53 10 10 10 10 0. 15. 40. 100. 0.0 1.7 0.9 3.0 0.00 11.58 2.31 2.97 10 10 10 10 EXPOSURE ON Ol AUG 01 MEAN 0. 15. 39. SD 0.0 1.2 0.9 CV(%) 0.00 7.62 2.25 N 10 10 10 CH. = CHAMBER, NO. = NUMBER, CONC. = CONCENTRATION CONCENTRATION IN: PPM 100. 1.3 1.29 10 PROJECT NO.: WIL 4170021 SPONSOR: HAP TASK FORCE INHALATION TABLE 6 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE DAILY MEAN TEST ATMOSPHERE CONCENTRATIONS PAGE 10 ____________________________________ GROUP: 0 PPM 15 PPM 40 PPM 100 PPM ____________________________________

CH. NO. 1 2. OM3 CH. NO. 2 2. OM3 CH. NO. 3 2. OM3 CH. NO. 4 2. OM3 CONC . CONC . CONC . CONC . _____________________________________

EXPOSURE ON 02 AUG 01 MEAN 0. 16. 40. 100. SD 0.0 3.5 1.3 1.9 CV(%) 0.00 22.28 3.34 1.91 N 10 10 10 10 EXPOSURE ON 03 AUG 01 MEAN 0. 15. 40. 100. Z(%) 0.00 0.0 20.45 3.1 2.36 0.9 3.20 3.2 N 10 10 10 10 EXPOSURE ON 06 AUG 01 $ SD MEAN 0. 0.0 % CV(%) N 0.00 10 cn z EXPOSURE ON 07 AUG 01 MEAN 0. SD 0.0 CV(%) 0.00 N 10 15. 39. PP. 1.7 3.4 1.6 11.02 8.73 1.58 10 10 10 15. 40. 100. 1.2 1.5 1.4 7.58 3.85 1.45 10 10 10 EXPOSURE ON OE AUG 01 MEAN 0. 15. 40. SD 0.0 1.9 1.3 CV(%) 0.00 12.27 3.21 N 10 10 10 CH. = CHAMBER, NO. = NUMBER, CONC. = CONCENTRATION CONCENTRATION IN: PPM 100. 1.8 1.76 10 PROJECT NO.: WIL 4170021 SPONSOR: HAP TASK FORCE INHALATION TABLE 6 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE DAILY MEAN TEST ATMOSPHERE CONCENTRATIONS PAGE 11 ____________________________________ GROUP: 0 PPM 15 PPM 40 PPM 100 PPM _________________^____________________ CH. NO. 1 2. OM3 CH. NO. 2 2. OM3 CH. NO. 3 2. OM3 CH. NO. 4 2. OM3 CONC . CONC . CONC . CONC .

EXPOSURE ON 09 AUG 01 MEAN 0. 15. 40. 100. SD 0.0 1.9 1.1 1.5 CV(%) 0.00 12.66 2.71 1.55 N 10 10 10 10 EXPOSURE ON lo AUG 01 MEAN 0. 16. 40. SD 0.0 3.7 1.4 CV(%) 0.00 22.95 3.42 N 10 10 10 EXPOSURE ON 13 AUG 01 MEAN 0. 16. 40. SD 0.0 1.4 0.9 CV(%) 0.00 9.25 2.31 N 10 10 10 EXPOSURE ON 15 AUG 01 MEAN 0. 15. 40. SD 0.3 1.1 3.7 CV(%) 316.23 7.21 9.29 N 10 10 10 EXPOSURE ON 17 AUG 01 MEAN 0. 16. 40. SD 0.0 2.6 0.8 CV(%) 0.00 16.14 2.15 N 10 10 10 CH. = CHAMBER, NO. = NUMBER, CONC. = CONCENTRATION CONCENTRATION IN: PPM 99. 1.4 1.44 10 100. 2.7 2.75 10 101. 2.7 2.70 10 99. 2.1 2.17 10 PROJECT NO.: WIL 4170021 SPONSOR: HAP TASK FORCE INHALATION TABLE 6 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE DAILY MEAN TEST ATMOSPHERE CONCENTRATIONS PAGE 12 __________________________________ GROUP: 0 PPM 15 PPM 40 PPM 100 PPM ___________________________________~~~~ CH. NO. 1 2. OM3 CH. NO. 2 2. OM3 CH. NO. 3 2. OM3 CH. NO. 4 2. OM3 CONC . CONC . CONC . CONC .

EXPOSURE ON 20 AUG 01 MEAN SD CV(%) N EXPOSURE ON 16 AUG 01 MEAN SD CV(%) N 0. 15. 39. 101. 0.0 1.9 0.8 2.0 0.00 12.66 2.01 2.00 10 10 10 10 0. 16. 39. 100. 0.0 1.4 6.7 3.3 0.00 8.62 17.39 3.28 9 10 10 10 EXPOSURE ON 14 AUG 01 MEAN 0. 16. 41. 101. SD 0.0 3.0 2.3 3.2 CV(%) 0.00 19.09 5.71 3.19 N 10 10 10 10 EXPOSURE ON 21 AUG 01 MEAN SD CV(%) N EXPOSURE ON 22 AUG 01 MEAN SD CV(%) N 0. 16. 40. 100. 0.0 2.1 0.5 0.9 0.00 13.18 1.30 0.95 10 10 I. 0 10 0. 17. 39. 100. 0.0 5.7 2.0 1.5 0.00 33.39 4.96 1.49 10 10 10 10 CH. = CHAMBER, NO. = NUMBER, CONC. = CONCENTRATION CONCENTRATION IN: PPM PROJECT NO.: WIL 4170021 SPONSOR: HAP TASK FORCE INHALATION TABLE 6 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE DAILY MEAN TEST ATMOSPHERE CONCENTRATIONS PAGE 13 GROUP: 0 PPM 15 PPM 40 PPM 100 PPM _____________________________________

CH. NO. 1 2. OM3 CH. NO. 2 2. OM3 CH. NO. 3 2. OM3 CH. NO. 4 2. OM3 CONC . CONC . CONC . CONC . _______________________________________

EXPOSURE ON 23 AUG 01 MEAN 0. SD 0.0 CV(%) 0.00 N 10 EXPOSURE ON 24 AUG 01 MEAN 0. EC%) 0.00 0.0 N 10 EXPOSURE ON 27 AUG 01 MEAN 0. SD 0.0 cv (k) 0.00 N 10 EXPOSURE ON 28 AUG 01 MEAN 0. El%) 0.00 0.0 N 10 EXPOSURE ON 29 AUG 01 MEAN 0. SD 0.0 CV(%) 0.00 N 10 CH. = CHAMBER, NO. = NUMBER, CONC. = CONCENTRATION CONCENTRATION IN: PPM 16. 39. 100. 2.8 1.1 1.9 17.68 2.83 1.85 10 10 10 16. 40. 100. 1.4 1.4 1.7 9.25 3.63 1.68 10 10 10 15. 39. 99. 1.4 1.3 2.0 9.07 3.43 2.04 10 10 10 16. 40. 100. 4.8 1.4 1.0 29.06 3.54 0.97 10 10 10 16. 39. 100. 1.6 0.8 1.9 9.98 2.18 1.93 10 10 10 411 of619 PROJECT NO.: WIL 4170021 SPONSOR: HAP TASK FORCE INHALATION TABLE 7 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK RATS & MICE DAILY ENVIRONMENTAL CONDITIONS FOR SECONDARY CHAMBER 4 (CH4 S) PAGE 1 DATE MEAN CHAMBER TEMPERATURE (DEGREES CELSIUS) MEAN CHAMBER RELATIVE HUMIDITY (%) MEAN CHAMBER VENTILATION RATE (SLPM) ______________________________________

06/ 25/ 01 21. 48. 387. 06/ 27/ 01 21. 46. 15s. 06/ 29/ 01 21. 45. 153. ____________________________________

GRAND MEAN 21. 46. 232. S. D. 0.0 1.5 135.5 C. V. (%) 0.0 3.26 58.0 N 3 3 3 MANUALv1.00 07/ 09/ 2001 R: 02/ 14/ 2002 P 413 of 619 WIL 417002 HAP Task Force 1, 1,2 Trichloroethane APPENDIX D Clinical Pathology Parameters for Control Animals (Study Week 8)

414 of 619 ,

415 of 619 I .

416 of 619 PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 1 SPONSOR: HAP TASK FORCE INDIVIDUAL SPECIAL CHEMISTRY VALUES WEEK 8 LACTATE ANIMAL 'GENASE _______________ U/ L GROUP: 0 PPM MALES 69302 440. 69306 513. 69308 349. 69310 421. 69312 418. 69314 335.

MEAN 413. S. D. 64.8 N 6 ______________________________________ U/ L = INTERNATIONAL UNIT/ LITER . PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 2 SPONSOR: HAP TASK FORCE INDIVIDUAL SPECIAL CHEMISTRY VALUES WEEK 8 _______^________________________________ LACTATE  ANIMAL 'GENASE ______________________________________

U/ L ___________________________________

GROUP: 0 PPM FEMALES 69354 268. 69366 273. 69367 460. 69378 283. 69384 281. 69398 232.

MEAN 300. S. D. 80.8 N 6 __________________ U/ L = INTERNATIONAL UNIT/ LITER PCHEv4.01 11/ 28/ 2001 WIL 417002 HAP Task Force APPENDIX E Pretest Clinical Observations 1, 1,2 Trichloroethane PRETEST CLINICAL OBSERVATIONS FOR RATS PROJECT NO.: WIL 417002P 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 1 SPONSOR: HAP TASK FORCE SUMMARY OF CLINICAL FINDINGS: TOTAL OCCURRENCE/ NO. OF ANIMALS _____ MALE _ ________________________________________

TABLE RANGE: 05 15 01 TO 05 30 01 GROUP: 1 ____________________________________~

NORMAL NO SIGNIFICANT CLINICAL OBSERVATIONS 142/ 49 DISPOSITION SCHEDULED SEROLOGY NECROPSY 51 5 EYES/ EARS/ NOSE RIGHT EYE MISSING 31 1 DRIED RED MATERIAL AROUND RIGHT EYE 21 1 __________________________________

l PRETEST 421 of 619 422of619 WIL 417002 HAP Task Force 1, 1,2 Trichloroethane APPENDIX F Serological Screening (BioReliance Corporation)

423 of 619 BIORELIANCE= Formerly Microbiological Associates BtORELtANCE CORPORATtON 14920 BROSCHART ROAD ROCKVtLLE, MARYLAND 20850 3349 USA PHONE: 301.738.1000 l FAX: 301.738.1036 TO: Our Code MR A079 Page 1 WIL 417002Q Susan Haley WIL Research Laboratories, Inc. 1407 George Road Ashland, OH 44605 FROM: Kathleen M. Vincent $kX&, %(&/ AC& DATE: May 30, 2001 SPECIMEN: 5 Mouse Sera RECEIVED: May 25, 2001 RESULTS: None of the tests were positive.

Sample ID __ &ISA ___ EDIM GDVII MHV MPul PVM Sendai II I IFA HA1 Sample ID Parvo MVM II ELISA: Positive value is >= 0.17 OD units IFA: + = positive;  = negative HAI: Numerical value = positive;  = negative 424of619 BIORELIANCE Formerly Microbiological Asociales BIOREL~ ANCE CORPoRAnON 4 Ec$ 4920 BROXHART ROAD J. 3;:. f& nq$ u, MAWLAND 20850 3349 USA Pi 40 301.738.? 000 l FAX: 301.738.1036 .

TO:

FROM: DATE: SPECIMHN: RFX! EIVED: Kathleen M. Vincent y~ cti~, TX. L, L~ J& May 30, 2001 10 Rat Sera May 25, 2001 RESULTS : None of the tests were positive. 1/ LIL /Q ' : : :Ql GT&+ Our Code MR A078 Page 1 WIL417002P Susan Haley WIL Research Laboratories, Inc. 1407 George Road Ashland, OH 44805 See attached tables for test results 425 of 619 426of619 W IL 4 17002 HAP Task Force 1, 1,2 Trichloroethane APPENDIX G Clinical Pathology Methods, Procedures and References 427of619 WIL 4 17002 HAP Task Force 1, 1,2 Trichloroethane CLINICAL PATHOLOGY METHODS, PROCEDURES AND REFERENCES Serum Chemistry  Hitachi 911 Albumin  Bromcresol Green (BCG) Method, Modification of the Doumas reaction. Hitachi 911 Application Code 413. Roche Reagent, catalog number 1970593.

A/ G Ratio  Calculated from Albumin and Globulin Results.

Alkaline Phosphatase  Kinetic reaction measuring the rate of p nitrophenol liberation. Hitachi 9 11 Application Code 0 16. Roche Reagent, catalog number 4500 12.

Alanine Aminotransferase  Modification of the International Federation of Clinical Chemistry (IFCC) recommended method. Hitachi 9 11 Application Code 033. Roche Reagent, catalog number 450065.

Aspartate Aminotransferase  Kinetic method based on the International Federation of Clinical Chemistry (IFCC) recommendations. Hitachi 911 Application Code 199. Roche Reagent, catalog number 450064.

Bilirubin (Total)  Diazo method developed by Wahlefeld et al. Stand J Clin Lab Invest 1972; 29: Supplement 126. Hitachi 911 Application Code 018. Roche Reagent, catalog number 1039034.

Blood Urea Nitrogen (BUN)  A urease triggered methodology based upon the method of Talke and Schubert Klin Wschr, 1965; 43: 174. Hitachi 911 Application Code 427. Roche Reagent, catalog number 148932 1.

Calcium  Modified method using a gamma amino butyric acid (GABA) buffer. Hitachi 9 11 Application Code . Roche Reagent, catalog number 1125621.

Chloride  An ion selective electrode that measures the electrical potential of the ions present in solution, Hitachi 911 Application Codes 097, 098, and 099. Roche Reagent, catalog numbers 450041,450042, and 450043.

Cholesterol  Enzymatic reaction as described by Trinder. Ann Clin Biochem 1974; 12: 266. Hitachi 9 11 Application Code 27 1. Roche Reagent, catalog number 45006 1.

Creatinine  Modified Jaffe reaction based on the work of Poper et al. Biochem 2 1937; 291: 354 and Seelig and Wuest, Aerztl Labor 1969; 15: 34. Hitachi 911 Application Code 077. Roche Reagent, catalog number 450019.

428 of 619 WIL 4 17002 HAP Task Force 1, 1,2 Trichloroethane Serum Chemistry (continued)

Gamma Glutamyltransferase (GGT)  Method based on the studies of Persijn and Van der Slik, J Clin Chem Clin Biochem, 1976; 14: 42 1. Hitachi 9 11 Application Code 173. Roche Reagent, catalog number 450067.

Globulin  Calculation obtained by subtracting Albumin from Total Protein.

Glucose  Glucose hexokinase method based on the work of Schmidt, Peterson and Young. Klin Wschr 1961; 39: 1244. Methods of Enzvrnatic Analvsis, Znd Eng ed. New York, Academic Press, 1974: 1196. Anal Biochemistrv 1958; 23: 301. Hitachi 911 Application Code 174. Roche Reagent, catalog number 450058.

Lactate Dehydrogenase (LD)  Modification of the Gay procedure using a Tris buffer. Methods of Enzvmatic Analysis, ed. 3. Weinheim, Verlag Chemie, 1983, Vol III, pp 126 133. Hitachi 911 Application Code 115. Roche Reagent, catalog number 450022.

Phosphorus  Method involves the formation of ammonium phosphomolybdate. Hitachi 911 Application Code 161. Roche Reagent, catalog number 450024.

Potassium  An ion selective electrode that measures the electrical potential of the ions present in solution. Hitachi 911 Application Codes 097, 098, and 099. Roche Reagent, catalog numbers 450041,450042, and 450043,

Sodium  An ion selective electrode that measures the electrical potential of the ions present in solution. Hitachi 911 Application Codes 097, 098, and 099. Boehringer Mannheim Reagent, catalog number 450041,450042, and 450043.

Total Protein  Endpoint biuret method that utilizes a sample blank. Hitachi 911 Application Code 166. Roche Reagent, catalog number 104090 1.

Triglycerides  Method that utilizes lipase from a microorganism to promote rapid and complete hydrolysis of triglycerides to glycerol. Hitachi 911 Application Code 006. Roche Reagent, catalog number 1488899.

Hematologv  CELL DYN' 3500 HCT  The Hematocrit is the ratio of red blood cells to plasma and is expressed as a percentage of the whole blood volume. The HCT is calculated from the red blood cell 429of619 WIL 4 17002 HAP Task Force 1, 1,2 Trichloroethane Hematology  CELL DYN' 3500( continued)

count and the mean cell volume as follows: HCT = (RBC x MCV)/ lO CELL DYN@ 3500 Operator's Manual. Abbott Diagnostics,

HGB  The Hemoglobin concentration is measured using a modified hemoglobincyanide method. A filtered LED with a wavelength of 540 m n is the light source. A photodetector measures the light that is transmitted. The Hemoglobin is directly measured and expressed in grams of hemoglobin per deciliter of whole blood. CELL DYN@ 3500 Operator's Manual. Abbott Diagnostics.

MCV  The Mean Cell Volume is the average volume of the individual red blood cells. The MCV is derived from the RBC size distribution data and is expressed in femtoliters. CELL DYN@ 3500 Operator's Manual. Abbott Diagnostics.

MCH  The Mean Corpuscular Hemoglobin is the average amount of hemoglobin contained in the red blood cell expressed in picograms. The MCH is calculated from the RBC and HGB as follows: MCH = (HGB/ RBC) x 10 CELL DYN@ 3 500 Operator's Manual. Abbott Diagnostics.

MCHC  The Mean Corpuscular Hemoglobin Concentration is the ratio of the weight of hemoglobin to the volume of the average red blood cell expressed in percent. It is calculated from the HGB and the HCT as follows: MCHC = (HGB/ HCT) x 100 CELL DYN' 3500 Operator's Manual. Abbott Diagnostics.

PLT  Platelets are counted and sized by the Aperture Impedance method. The Platelet Count is derived from the histogram after the PLT data has been analyzed by the platelet algorithm. The Platelet Count is expressed as #xlO'/~ l. CELL DYN@ 3500 Operator's Manual. Abbott Diagnostics.

RBC Count  The Red Blood Cells are counted and sized by the Aperture Impedance method. The Red Blood Cell Count is directly measured and given the number of RBC's. The RBC Count is expressed as #x106/ yl. CELL DYN' 3500 Operator's Manual. Abbott Diagnostics.

430 of 619 WIL 4 17002 HAP Task Force 1, 1,2 Trichloroethane Hematology  CELL DYN@ 3500( continued)

WBC Count  The White Blood Cell Count is analyzed in two separate channels. Both an optical and impedance analysis are performed. Combining both methodologies minimizes any interference caused by the presence of fragile WBCs, NRBCs or lyse resistant RBCs. The WBC Count is expressed as #x103/ uL. CELL DYN@ 3500 Operator's Manual. Abbott Diagnostics.

WBC Differential Count  The light scatter information is graphically presented in the form of scatterplots. Each cell analyzed is represented by a dot on the scatterplot. The dots are plotted at a point determined by the intersection of the channel information designated on the X and Y axes. The scatter inforrnation may be plotted in various combinations to yield different information. The CELL DYN' 3500 uses the scatter plots to differentiate the WBCs into five subpopulations: Neutrophils, Eosinophils, Lymphocytes, Basophils and Monocytes. CELL DYN@ 3500 Operator's Manual. Abbott Diagnostics.

Hematology  Manual Methods White Cell Differential  Manual method of counting 100 white cells stained with Wright Giemsa and entered on line into the data files.

Reticulocyte Count  Manual method of counting the reticulocytes present in 1000 red blood cells stained with New Methylene Blue and entered into the data files.

Red Blood Cell Morphology  Manual method of evaluating red blood cells on a Wright Giemsa stained slide and entered into the data files.

Coagulation Prothrombin Time (Protime PT)  Photometric clot detection is used to measure and record the time required for plasma specimens to clot by adding an excess of thromboplastin reagent and an optional amount of calcium to titrated plasma. Automated Coagulation Analyzer Electra 1400C. Medical Laboratory Automation, Inc.

Activated Partial Thromboplastin Time (APTT)  Photometric clot detection is used to measure and record clotting time in a one stage procedure which consists of recalcifying plasma in the presence of an excess of platelet like reagent (cephalin) containing a plasma activator. Automated Coagulation Analyzer Electra 14OOC. Medical Laboratory Automation, Inc.

431 of 619 WIL 4 17002 HAP Task Force 1, 1,2 Trichloroethane APPENDIX H Individual Clinical Observations 432 of 619 PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL CLINICAL OBSERVATIONS TABLE RANGE: 05 31 01 TO 08 31 01 ANIMAL SEX GROUP CATEGORY ___^______________________________ 69302 M 0 PPM NORMAL DATE TIME GRADE OBSERVATIONS 06 06 01 7~ 27 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 6: 58 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 6: 40 p NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 30 p NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 07 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 28 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 6: 56 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 6: 47 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 23 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 19 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 22 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 58 P PRIMARY NECROPSY (WEEK 13) 07 11 01 6: 16 P RED DISCHARGE LEFT EYE 08 30 01 7: 38 P OPACITY LEFT EYE 06 06 01 7: 31 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 6: 58 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 6: 41 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 30 P ~0 SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 08 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 17 P NO SIGNIFICANT CLINICAL OBBERVATIONS 07 18 01 7: 28 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 6: 56 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 23 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 19 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 23 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 34 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 56 P PRIMARY NECROPSY (WEEK 13) 08 01 01 6: 48 P RED DISCHARGE RIGHT EYE 06 06 01 7: 31 P NO SIGNIFICANT CLINICAL OBSERVATIONS PAGE 1 69302 M 0 PPM DISPOSITION 69302 M 0 PPM EYES/ EARS/ NOSE 69306 M 0 PPM NORMAL 69306 M 0 PPM DISPOSITION 69306 M 0 PPM EYES/ EARS/ NOSE 69308 M 0 PPM NORMAL GRADE CODE: 1  SLIGHT 2  MODERATE 3  SEVERE P  PRESENT DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 2 S?? ONSOR: HAP TASK FORCE INDIVIDUAL CLINICAL OBSERVATIONS ANIMAL SEX ________ 69308 M 69308 M 69308 M 69310 M % ch 69310 M G 69310 M 69310 M 69312 M ____ GRADE CODE: 1 GROUP 0 PPM 0 PPM 0 PPM 0 PPM 0 PPM 0 PPM 0 PPM 0 PPM TABLE RANGE: 05 31 01 TO 08 31 01 CATEGORY ________ NORMAL _  DATE TIME GRADE OBSERVATIONS ____________________________________

06 13 01 6: 58 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 6: 41 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 30 P ~0 SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 08 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 18 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 29 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 6: 56 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 6: 48 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 23 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 19 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 23 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 55 p PRIMARY NECROPSY (WEEK 13) 08 30 01 7: 32 P DRIED RED MATERIAL AROUND LEFT EYE 06 06 01 7: 31 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 30 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 08 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 29 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 6: 57 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 24 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 20 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 31 01 7: 44 P PRIMARY NECROPSY (WEEK 13) 07 11 01 6: lP P SOFT FECES 06 13 01 6: 59 P TAIL BROKEN 06 20 01 6: 42 P TAIL BROKEN 08 01 01 6: 48 P TAIL BROKEN 08 22 01 6: 24 P TAIL BROKEN 08 31 01 7: 07 P TAIL BROKEN 06 06 01 7: 32 P NO SIGNIFICANT CLINICAL OBSERVATIONS DISPOSITION EYES/ EARS/ NOSE NORMAL DISPOSITION EXCRETA SPECIAL II NORMAL ____________________________________ SLIGHT 2  MODERATE 3  SEVERE P  PRESENT DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE SPONSOR: HAP TASK FORCE PAGE 3 INDIVIDUAL CLINICAL OBSERVATIONS TABLE RANGE: 05 31 01 TO 08 31 01 ___________________________________~~ ANIMAL SEX GROUP CATEGORY DATE TIME GRADE OBSERVATIONS _____________________________________~~

69312 M 0 PPM NORMAL 06 13 01 6: 59 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 6: 42 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 30 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 08 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 20 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 29 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 6: 5 l P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 6: 49 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 24 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 20 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 24 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 31 01 7~ 08 P NO SIGNIFICANT CLINICAL OBSERVATIONS 69312 M 0 PPM DISPOSITION 08 31 01 7: 44 69314 M 0 PPM P PRIMARY NECROPSY (WEEK 13) NORMAL 06 06 01 7: 32 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: oo P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 6: 42 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 30 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 08 P NO SIGNIFICANT CLINICAL OBSERVATIONS % 07 25 01 6~ 57 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 6: 49 P NO SIGNIFICANT CLINICAL OBSERVATIONS o\ 08 08 01 6: 24 P NO SIGNIFICANT CLINICAL OBSERVATIONS E 08 15 01 7: 21 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 25 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 31 01 7: 08 P NO SIGNIFICANT CLINICAL OBSERVATIONS 69314 M 0 PPM DISPOSITION 08 31 01 7: 45 P PRIMARY NECROPSY (WEEK 13) 69314 M 0 PPM EYES/ EARS/ NOSE 07 11 01 6: 21 P DRIED RED MATERIAL AROUND LEFT EYE 07 11 01 6: 21 P RED DISCHARGE LEFT EYE 07 18 01 7: 30 P DRIED RED MATERIAL AROUND LEFT EYE 69317 M 0 PPM NORMAL 06 06 01 7: 33 P NO SIGNIFICANT CLINICAL OBSERVATIONS ___________________________________ GRADE CODE: 1  SLIGHT 2  MODERATE 3  SEVERE P  PRESENT DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 4 SPONSOR: HAP TASK FORCE INDIVIDUAL CLINICAL OBSERVATIONS TABLE RANGE: 05 31 01 TO 08 31 01 ____________ ANIMAL SEX GROUP CATEGORY DATE TIME GRADE OBSERVATIONS ______________~~~~~~~~~~ 69317 M 0 PPM NORMAL 69317 M 0 PPM DISPOSITION 69322 M 0 PPM NORMAL 69322 M 0 PPM DISPOSITION 69337 M 0 PPM NORMAL ________________ GRADE CODE: 1  SLIGHT 2  MODERATE 3 06 13 01 7: oo P 06 20 01 6: 42 P 06 27 01 6: 31 P 07 04 01 6: 09 P 07 11 01 6: 22 P 07 18 01 7: 30 P 07 25 01 6: 58 P 08 01 01 6: 50 P 08 08 01 6: 24 P 08 15 01 7: 21 P 08 22 01 6: 25 P 08 31 01 7: 08 P 08 31 01 7: 45 P 06 06 01 7: 33 P 06 13 01 7: 00 P 06 20 01 6: 43 P 06 27 01 6: 31 P 07 04 01 6: 09 P 07 11 01 6: 23 P 07 18 01 7: 30 P 07 25 01 6: 58 P 08 01 01 6: 50 P 08 08 01 6: 25 P 08 15 01 7: 22 P 08 22 01 6: 25 P 08 31 01 7: 08 P 08 31 01 7: 45 P 06 06 01 7: 33 P 06 13 01 7: 00 P _________________.

SEVERE P  PRESENT NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS PRIMARY NECR~ PSY (WEEK 13) NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS PRIMARY NECROPSY (WEEK 13) NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS __ ______ __ DETAILED FHYSICAL EXAMINATIONS/ DISPOSITIONS PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 5 SFONSOR: HAF TASK FORCE INDIVIDUAL CLINICAL OBSERVATIONS TABLE RANGE: 05 31 01 TO 08 31 01 _________________~~ ANIMAL SEX GROUP CATEGORY DATE TIME GRADE OBSERVATIONS 69337 M 0 PPM NORMAL 06 20 01 6: 43 F NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 31 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 09 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 23 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 30 F NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 6: 58 F NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 6: 50 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 25 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 22 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 33 P NO SIGNIFICANT CLINICAL OBSERVATIONS 69337 M 0 FFM DISPOSITION 08 30 01 7: 56 P PRIMARY NECROPSY (WEEK 13) 69337 M 0 FFM EXCRETA 08 22 01 6: 26 F DECREASED DEFECATION 69337 M 0 PPM SPECIAL 08 22 01 6: 26 F WET BROWN MATERIAL ANOGENITAL AREA 69343 M 0 FPM NORMAL 06 06 01 7: 34 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: Ol F NO SIGNIFICANT CLINICAL OBSERVATIONS pw 06 20 01 6: 43 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 31 P NO SIGNIFICANT CLINICAL OBSERVATIONS 4 07 04 01 6: 09 F NO SIGNIFICANT CLINICAL OBSERVATIONS 4 07 11 01 6: 24 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 31 P NO SIGNIFICANT CLINICAL OBSERVATIONS cI\ 07 25 01 6: 59 F NO SIGNIFICANT CLINICAL OBSERVATIONS t; 08 01 01 6: 51 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 25 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 36 F NO SIGNIFICANT CLINICAL OBSERVATIONS 69343 M 0 PFM DISPOSITION 08 30 01 7: 57 P PRIMARY NECROPSY (WEEK 13) 69343 M 0 PPM EYES/ EARS/ NOSE 08 15 01 7: 23 P DRIED RED MATERIAL AROUND RIGHT EYE 08 15 01 7: 23 P DRIED RED MATERIAL AROUND LEFT EYE 69343 M 0 PPM SPECIAL 08 15 01 7: 23 P WET YELLOW MATERIAL UROGENITAL AREA 08 22 01 6: 27 F DRIED YELLOW MATERIAL UROGENITAL AREA ______________

GRADE CODE: 1  SLIGHT 2  MODERATE 3  SEVERE P  PRESENT DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE SPONSOR: HAP TASK FORCE PAGE 6 INDIVIDUAL CLINICAL OBSERVATIONS TABLE RANGE: 05 31 01 TO 08 31 01 ___________________________________ ANIMAL SEX GROUP CATEGORY DATE TIME GRADE OBSERVATIONS _____________________________________~ 69305 M 15 PPM NORMAL 06 06 01 7: 38 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 04 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 6: 47 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 33 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: ll P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 30 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 35 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: 04 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 6: 58 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 29 p NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 28 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 33 P NO SIGNIFICANT CLINICAL OBSERVATIONS 69305 M 15 PPM DISPOSITION 08 30 01 7: 56 P 69305 M 15 PPM PRIMARY NECROPSY (WEEK 13) EYES/ EARS/ NOSE 08 30 01 7: 34 P DRIED RED MATERIAL AROUND RIGHT EYE 69316 M 15 PPM NORMAL 06 06 01 7: 38 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 04 P NO SIGNIFICANT CLINICAL OBSERVATIONS is 06 20 01 6: 48 P NO SIGNIFICANT CLINICAL OBSERVATIONS co 06 27 01 6: 33 P NO SIGNIFICANT CLINICAL OBSERVATIONS 0 07 04 01 6: ll P NO SIGNIFICANT CLINICAL OBSERVATIONS 2 07 11 01 6: 31 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 35 P NO SIGNIFICANT CLINICAL OBSERVATIONS z 07 25 01 7: 04 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 6: 58 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 30 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7~ 28 p NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 33 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 32 P NO SIGNIFICANT CLINICAL OBSERVATIONS 69316 M 15 PPM DISPOSITION 08 30 01 7: 55 P PRIMARY NECR~ PSY (WEEK 13) 69320 M 15 PPM NORMAL 06 06 01 7: 39 P NO SIGNIFICANT CLINICAL OBSERVATIONS _____________________________________

GRADE CODE: 1  SLIGHT 2  MODERATE 3  SEVERE p  PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORcE ANIMAL SEX ____^_______ 69320 M ___________________

_____ GROUP .____ __

15 PPM 69320 M 15 PPM 69320 M 15 PPM 69323 M 15 PPM 69323 M 69323 M 15 PPM 15 PPM DETAILED PHYSICAL EKAMINATIONS/ DISPOSITIONS 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL CLINICAL OBSERVATIONS PAGE 7 TABLE RANGE: 05 31 01 TO 08 31 01 CATEGORY _______ NORMAL DATE TIME GRADE OBSERVATIONS _ _ _ _ _ _ _ _ _ _____________________________________ 06 13 01 7: 05 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 6~ 48 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 33 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: ll P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 31 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 35 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 6: 58 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 30 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 29 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 33 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 31 01 7: 08 p NO SIGNIFICANT CLINICAL OBSERVATIONS 08 31 01 7: 45 p PRIMARY NECROPSY (WEEK 13) 07 25 01 7: 05 P SOFT DISPOSITION EXCRETA DISPOSITION EYES/ EARS/ NOSE FECES 06 06 01 7: 39 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 05 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 6: 48 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 34 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 12 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 32 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7~ 35 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: 05 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 30 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 29 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 34 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 31 01 7: 45 P PRIMARY NECROPSY (WEEK 13) 08 01 01 6: 59 P DRIED RED MATERIAL AROUND LEFT EYE 08 01 01 6: 59 P RED DISCHARGE LEFT EYE 08 31 01 7: 08 P DRIED RED MATERIAL AROUND LEFT EYE _____________________________________~~~~ GRADE CODE: 1  SLIGHT 2  MODERATE 3  SEVERE P  PRESENT PROJECT NO.: WIL 417002 SP~ NSOR: HAP TASK FORCE DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL CLINICAL OBSERVATIONS PAGE 8 TABLE RANGE: 05 31 01 TO 08 31 01 ANIMAL sEx GROUP CATEGORY DATE TIME GRADE OBSERVATIONS 69326 M 15 PPM NORMAL 06 06 01 7: 39 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: os P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 6: 49 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 34 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 12 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 32 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 36 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: 05 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 6: 59 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 30 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 30 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 34 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 35 P NO SIGNIFICANT CLINICAL OBSERVATIONS 69326 M 15 PPM DISPOSITION 08 30 01 7: 56 p PRIMARY NECROPSY (WEEK 1.3) 69335 M 15 PPM NORMAL 06 06 01 7: 40 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 05 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 6: 49 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 34 P NO SIGNIFICANT CLINICAL OBSERVATIONS 0 07 04 01 6: 12 P NO SIGNIFICANT CLINICAL OBSERVATIONS z 07 11 01 6: 33 P NO SIGNIFICANT CLINICAL OBSERVATIONS 5 07 18 01 7: 36 P NO SIGNIFICANT CLINICAL 07 25 01 7: 06 P NO SIGNIFICANT CLINICAL OBSERVATIONS OBSERVATIONS 08 01 01 7: 00 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 31 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 30 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 35 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 31 01 7: 08 P NO SIGNIFICANT CLINICAL OBSERVATIONS 69335 M 15 PPM DISPOSITION 08 31 01 7: 45 P PRIMARY NECROPSY (WEEK 13) 69336 M 15 PPM NORMAL 06 06 01 7: 40 P NO SIGNIFICANT CLINICAL OBSERVATIONS _____________________________________~~

GRADE CODE: 1  SLIGHT 2  MODERATE 3  SEVERE P  PRESENT DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 9 SPONSOR: HAP TASK FORCE INDIVIDUAL CLINICAL OBSERVATIONS TABLE RANGE: 05 31 01 TO 08 31 01 ___________________________________~

ANIMAL SEX _______

69336 M 69336 M 69338 M 69338 M 69338 M 69345 M GROUP CATEGORY _________ _______________

15 PPM NORMAL 15 PPM 15 PPM 15 PPM 15 PPM 15 PPM DISPOSITION NORMAL DISPOSITION EYES/ EARS/ NOSE NORMAL DATE TIME GRADE OBSERVATIONS _____________~~

06 13 01 7: 06 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 6: 50 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 35 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 13 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 33 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 36 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7 : 06 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 7: 00 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 31 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 30 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 35 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 31 01 7: 08 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 31 01 7: 45 P PRIMARY NECROPSY (WEEK 13) 06 06 01 7: 41 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 06 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 6: 50 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 35 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 13 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 34 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 36 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 7: Ol P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 31 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 31 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 35 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 58 P PRIMARY NECROPSY (WEEK 13) 07 25 01 7: 06 P DRIED RED MATERIAL AROUND LEFT EYE 08 30 01 7: 38 P RED DISCHARGE LEFT EYE 06 06 01 7: 41 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 06 P NO SIGNIFICANT CLINICAL OBSERVATIONS ____________________________^_______~~~~ GRADE CODE: 1  SLIGHT 2  MODERATE 3  SEVERE P  PRESENT PROJECT NO.: WIL 417002 DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 10 TABLE RANGE: 05 31 01 TO 08 31 01 ____________________________________ ANIMAL SEX GROUP CATEGORY DATE TIME GRADE OBSERVATIONS ___________________________________ 69345 M 15 PPM NORMAL 06 20 01 6: 51 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 35 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 13 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 34 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 36 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: 07 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 7: Ol P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 31 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 31 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 35 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 31 01 7: 08 P NO SIGNIFICANT CLINICAL OBSERVATIONS 69345 M 15 PPM DISPOSITION 08 31 01 7~ 45 P PRIMARY NECROPSY (WEEK 13) 69347 M 15 PPM NORMAL 06 06 01 7: 42 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 07 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 6: 51 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 35 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 13 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 35 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 37 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: 07 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 7: Ol P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 32 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 31 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 36 P NO SIGNIFICANT CLINICAL OBSERVATIONS 69347 M 15 PPM DISPOSITION 08 30 01 7: 56 P PRIMARY NECROPSY (WEEK 13) 69347 M 15 PPM EYES/ EARS/ NOSE 08 30 01 7: 35 P RED DISCHARGE LEFT EYE 69303 M 40 PPM NORMAL 06 06 01 7: 48 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: ll P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 6: 56 P NO SIGNIFICANT CLINICAL OBSERVATIONS SPONSOR: HAP TASK FORCE INDIVIDUAL CLINICAL OBSERVATIONS GRADE CODE: 1  SLIGHT 2  MODERATE 3  SEVERE P  PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL CLINICAL OBSERVATIONS PAGE 11 TABLE RANGE: 05 31 01 TO 08 31 01 ____________________________________ ANIMAL SEX GROUP CATEGORY DATE TIME GRADE OBSERVATIONS 69303 M 40 PPM 69303 M 40 PPM 69309 M 40 PPM 69309 M 40 PPM 69313 M 40 PPM ______________________ GRADE CODE: 1  SLIGHT 2 NORMAL 06 27 01 6: 37 P 07 04 01 6: 16 P 07 11 01 6: 4l. P 07 18 01 7: 39 P 07 25 01 7: ll P 08 01 01 7: 05 P 08 08 01 6: 35 P 08 15 01 7: 35 P 08 22 01 6: 38 P 08 30 01 7: 36 P DISPOSITION 08 30 01 7: 57 P NORMAL 06 06 01 7: 48 P 06 13 01 7: ll P 06 20 01 6: 56 P 06 27 01 6: 37 P 07 04 01 6: 16 P 07 11 01 6: 41 P 07 18 01 7: 39 P 07 25 01 7: ll P 08 01 01 7: 05 P 08 08 01 6: 36 P 08 15 01 7: 36 P 08 22 01 6: 38 P 08 31 01 7: 08 P DISPOSITION 08 31 01 7: 45 P NORMAL 06 06 01 7: 48 P 06 13 01 7: ll P 06 20 01 6: 57 P 06 27 01 6: 38 P _________________ c_______________

MODERATE 3  SEVERE P  PRESENT NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS PRIMARY NECROPSY (WEEK 13) NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS PRIMARY NECROPSY (WEEK 13) NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS _____________________________________~~~~ PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL CLINICAL OBSERVATIONS PAGE 12 TABLE RANGE: 05 31 01 TO 08 31 01 ____________________________________~~~ ANIMAL SEX GROUP CATEGORY DATE TIME GRADE OBSERVATIONS 69313 M 40 PPM NORMAL 69313 M 40 PPM DISPOSITION 69313 M 40 PPM EYES/ EARS/ NOSE 69313 M 40 PPM ORAL/ DENTAL 69315 M 40 PPM NORMAL 69315 M 40 PPM DISPOSITION 69324 M 40 PPM NORMAL 07 11 01 6: 41 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 39 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6~ 36 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 36 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 39 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 37 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 58 P PRIMARY NECROPSY (WEEK 13) 07 04 01 6: 16 P DRIED RED MATERIAL AROUND LEFT EYE 07 25 01 7: 12 P UPPER INCISORS MALALIGNED 08 01 01 7: 07 P UPPER INCISORS MALALIGNED 08 01 01 7: 08 P LOWER INCISOR( S) LONG, TRIMMED 06 06 01 7: 49 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 12 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 6157 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 38 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6~ 17 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6~ 42 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 39 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 I: 12 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 7~ 08 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 36 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 36 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 39 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 38 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 57 P PRIMARY NECROPSY (WEEK 13) 06 06 01 7: 49 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 38 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: 13 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6~ 36 P NO SIGNIFICANT CLINICAL OBSERVATIONS _______________________________________ GRADE CODE: 1  SLIGHT 2  MODERATE 3  SEVERE P  PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL CLINICAL OBSERVATIONS PAGE 13 TABLE RANGE: 05 31 01 TO 08 31 01 ____________________________________~~~ ANIMAL SEX GROUP CATEGORY DATE TIME GRADE OBSERVATIONS _

69324 M 40 PPM NORMAL 69324 M 40 PPM DISPOSITION 69324 M 40 PPM EYES/ EARS/ NOSE 69324 M 40 PPM BODY/ INTEG II 69324 M 40 PPM SPECIAL II ti % 69328 M 40 PPM cn G NORMAL 08 15 01 7: 37 P 08 31 01 7: 45 P 07 11 01 6: 43 P 07 11 01 6~ 43 P 08 01 01 7: 09 P 08 22 01 6: 39 P 08 31 01 7: OY P 06 13 01 7: 12 P 06 20 01 6: 58 P 07 11 01 6: 43 P 06 13 01 7: 12 P 06 20 01 6: 58 P 07 04 01 6: 17 P 07 11 01 6: 43 P 07 18 01 7: 40 P 08 01 01 7: 09 P 08 22 01 6: 39 P 08 31 01 7: 09 P 06 06 01 7: 50 P 06 13 01 7: 12 P 06 20 01 6: 58 P 06 27 01 6: 38 P 07 04 01 6: 17 P 07 11 01 6~ 43 P 07 18 01 7: 40 P 07 25 01 7: 13 P 08 01 01 7: 09 P 08 08 01 6~ 37 P 08 15 01 7: 37 P _________________________________~ NO SIGNIFICANT CLINICAL OBSERVATIONS PRIMARY NECROPSY (WEEK 13) DRIED RED MATERIAL AROUND LEFT EYE RED DISCHARGE LEFT EYE RED DISCHARGE LEFT EYE DRIED RED MATERIAL AROUND LEFT EYE DRIED RED MATERIAL AROUND LEFT EYE SCABBING END OF TAIL SCABBING END OF TAIL SCABBING END OF TAIL END OF TAIL MISSING END OF TAIL MISSING END OF TAIL MISSING END OF TAIL MISSING END OF TAIL MISSING END OF TAIL MISSING END OF TAIL MISSING END OF TAIL MISSING NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS _____________________________________ GRADE CODE: 1  SLIGHT 2  MODERATE 3  SEVERE P  PRESENT DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 14 SPONSOR: HAP TASK FORCE INDIVIDUAL CLINICAL OBSERVATIONS TABLE RANGE: 05 31 01 TO 08 31 01 ANIMAL SEX GROUP CATEGORY DATE TIME GRADE OBSERVATIONS _______________________________________

69328 M 40 PPM NORMAL 08 22 01 6: 40 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 31 01 7: 09 P NO SIGNIFICANT CLINICAL OBSERVATIONS 69328 M 40 PPM DISPOSITION 08 31 01 7: 45 P PRIMARY NE~ ROPS~ (WEEK 13) 69339 M 40 PPM NORMAL 06 06 01 7: 50 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 13 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 6: 58 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 38 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6~ 17 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 44 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 40 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: 13 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 7: lO P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 37 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 37 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 40 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 34 P NO SIGNIFICANT CLINICAL OBSERVATIONS t 69339 M 40 PPM DISPOSITION 08 30 01 8: 01 P PRIMARY NECROPSY (WEEK 13) cn 69344 M 40 PPM NORMAL 06 06 01 7: 50 P NO SIGNIFICANT CLINICAL OBSERVATIONS % 06 13 01 7: 13 P NO SIGNIFICANT CLINICAL OBSERVATIONS cn 06 20 01 6: 59 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 38 P NO SIGNIFICANT CLINICAL OBSERVATIONS 5 07 04 01 6: 18 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 44 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 40 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: 14 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 7: lO P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 37 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 38 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 40 P NO SIGNIFICANT CLINICAL OBSERVATIONS ________________________________________

GRADE CODE: 1  SLIGHT 2  MODERATE 3  SEVERE P  PRESENT PAGE 15 PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL CLINICAL OBSERVATIONS TABLE RANGE: 05 31 01 TO 08 31 01 _____________________________________ ANIMAL SEX GROUP CATEGORY DATE TIME GRADE OBSERVATIONS ____________________________________

69344 M 40 PPM NORMAL 08 31 01 7: 09 P NO SIGNIFICANT CLINICAL OBSERVATIONS 69344 M 40 PPM DISPOSITION 08 31 01 7: 45 P PRIMARY NECROPSY (WEEK 13) 69348 M 40 PPM NORMAL 06 06 01 7: 51 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 13 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 6: 59 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 39 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 18 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 45 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: 14 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 7: lO P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 37 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 38 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 40 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 31 P NO SIGNIFICANT CLINICAL OBSERVATIONS 69348 M 40 PPM DISPOSITION 08 30 01 7: 55 P PRIMARY NECROPSY (WEEK 13) 69348 M 40 PPM SPECIAL 07 18 01 7~ 41 P WET BROWN MATERIAL ANOGENITAL AREA 69350 M 40 PPM NORMAL 06 06 01 7: 51 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 14 P NO SIGNIFICANT CLINICAL OBSERVATIONS % 06 20 01 6: 59 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 39 P NO SIGNIFICANT CLINICAL OBSERVATIONS CR 07 04 01 6: 18 P NO SIGNIFICANT CLINICAL OBSERVATIONS 5, 07 11 01 6: 45 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 41 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: 14 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 7: ll P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 37 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 38 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 40 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 31 01 7: 09 P NO SIGNIFICANT CLINICAL OBSERVATIONS GRADE CODE: 1  SLIGHT 2  MODERATE 3  SEVERE P  PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL CLINICAL OBSERVATIONS PAGE 16 TABLE RANGE: 05 31 01 TO 08 31 01 ______________~~~~~~ ANIMAL SEX GROUP CATEGORY DATE TIME GRADE OBSERVATIONS _______________________^_________

69350 M 40 PPM DISPOSITION 08 31 01 7: 46 P PRIMARY NECROPSY (WEEK 13) 69304 M 100 PPM NORMAL 06 06 01 7~ 56 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 17 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 7: 05 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 41 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 20 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 52 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7~ 46 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: 18 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 7: 15 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 41 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 43 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 43 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 31 01 7: 09 P NO SIGNIFICANT CLINICAL OBSERVATIONS 69304 M 100 PPM DISPOSITION 08 31 01 7: 46 P PRIMARY NECROPSY (WEEK 13) 69307 M 100 PPM NORMAL 06 06 01 7: 56 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 17 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 7: 05 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 42 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 21 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 52 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 46 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: 18 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 41 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 43 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 44 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 35 P NO SIGNIFICANT CLINICAL OBSERVATIONS 69307 M 100 PPM DISPOSITION 08 30 01 7: 56 p PRIMARY NEcROPSY (WEEK 13) 69307 M 100 PPM EYES/ EARS/ NOSE 08 01 01 7: 16 P DRIED RED MATERIAL AROUND RIGHT EYE ____________________________________~ GRADE CODE; 1  SLIGHT 2  MODERATE 3  SEVERE P  PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL CLINICAL OBSERVATIONS PAGE 17 TABLE RANGE: 05 31 01 TO 08 31 01 _______________________________________ ANIMAL SEX GROUP CATEGORY DATE TIME GRADE OBSERVATIONS __ 69318 M 100 PPM NORMAL 06 06 01 '7~ 56 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 17 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 7: 05 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6~ 42 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 21 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 53 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 46 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: 19 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 7: 16 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 41 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 43 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 36 P NO SIGNIFICANT CLINICAL OBSERVATIONS 69318 M 100 PPM DISPOSITION 08 30 01 7: 57 P PRIMARY NECROPSY (WEEK 13) 69318 M 100 PPM SPECIAL 08 22 01 6: 44 P WET YELLOW MATERIAL UROGENITAL AREA 69319 M 100 PPM NORMAL 06 06 01 7: 57 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 18 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 7: 06 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 42 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 21 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 53 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 47 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: 19 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 7: 17 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 42 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 44 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6~ 44 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 31 01 7: 09 P NO SIGNIFICANT CLINICAL OBSERVATIONS 69319 M 100 PPM DISPOSITION 08 31 01 7: 46 P PRIMARY NECROPSY (WEEK 13) 69327 M 100 PPM NORMAL 06 06 01 7: 57 P NO SIGNIFICANT CLINICAL OBSERVATIONS _______________________________________~~~~~~ GRADE CODE: 1  SLIGHT 2  MODERATE 3  SEVERE P  PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL CLINICAL OBSERVATIONS PAGE 18 TABLE RANGE: 05 31 01 TO 08 31 01 ___________________________________~~ ANIMAL SEX GROUP CATEGORY DATE TIME GRADE OBSERVATIONS ___________________________________~~~

69327 M 100 PPM NORMAL 06 13 01 7: 18 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 7: 06 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 42 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 22 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 54 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 47 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: 19 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 7: 17 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 42 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 44 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 44 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 31 01 7: 09 P NO SIGNIFICANT CLINICAL OBSERVATIONS 69327 M 100 PPM DISPOSITION 08 31 01 7~ 46 p PRIMARY NECROPSY (WEEK 13) 69329 M 100 PPM NORMAL 06 06 01 7~ 58 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 18 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 7: 06 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 43 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 23 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 54 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 48 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: 20 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 7: 18 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 42 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 44 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 44 P NO SIGNIFICANT CLINICAL OBSERVATIONS 69329 M 100 PPM DISPOSITION 08 30 01 7: 58 p PRIMARY NECROPSY (WEEK 13) 69329 M 100 PPM EYES/ EARS/ NOSE 08 30 01 7: 39 P RED DISCHARGE RIGHT EYE 69332 M 100 PPM NORMAL 06 06 01 7: 58 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 18 P NO SIGNIFICANT CLINICAL OBSERVATIONS ________________________________________ GRADE CODE: 1  SLIGHT 2  MODERATE 3  SEVERE P  PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL CLINICAL OBSERVATIONS PAGE 19 ANIMAL SEX GROUP CATEGORY DATE TIME GRADE OBSERVATIONS ___________________________________

69332 M 100 PPM NORMAL 06 20 01 7: 07 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 43 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 24 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 55 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 48 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: 20 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 7: 18 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 42 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 44 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 45 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 31 01 7: 09 P NO SIGNIFICANT CLINICAL OBSERVATIONS 69332 M 100 PPM DISPOSITION 08 31 01 7~ 46 P PRIMARY NECROPSY (WEEK i3) 69334 M 100 PPM NORMAL 06 06 01 I: 58 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 19 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 7: 08 P NO SIGNIFICANT CLINICAL OBSERVATIONS R 06 27 01 6: 43 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 24 P NO SIGNIFICANT CLINICAL OBSERVATIONS B 07 11 01 6: 55 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 48 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: 20 P NO SIGNIFICANT CLINICAL OBSERVATIONS m G 08 01 01 7: 18 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 42 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 45 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 45 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 34 P NO SIGNIFICANT CLINICAL OBSERVATIONS 69334 M 100 PPM DISPOSITION 08 30 01 7: 56 P PRIMARY NECROPSY (WEEK 13) 69342 M 100 PPM NORMAL 06 06 01 7: 59 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 19 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 7: 08 P NO SIGNIFICANT CLINICAL OBSERVATIONS TABLE RANGE: 05 31 01 TO 08 31 01 GRADE CODE: 1  SLIGHT 2  MODERATE 3  SEVERE P  PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL CLINICAL OBSERVATIONS PAGE 20 TABLE RANGE: 05 31 01 TO 08 31 01 _______________________________________~~~~~~~~ ANIMAL SEX GROUP CATEGORY DATE TIME GRADE OBSERVATIONS __. _________ 69342 M 69342 M 100 PPM DISPOSITION 69346 M 100 PPM NORMAL 0, cn ri 69346 M 69346 M 69354 F 100 PPM NORMAL 100 PPM DISPOSITION 100 PPM EYES/ EARS/ NOSE 0 PPM NORMAL 06 27 01 07 04 01 07 11 01 07 18 01 07 25 01 08 01 01 08 08 01 08 15 01 08 22 01 08 31 01 08 31 01 06 06 01 06 13 01 06 20 01 06 27 01 07 04 01 07 11 01 07 18 01 07 25 01 08 08 01 08 15 01 08 30 01 08 01 01 08 22 01 08 30 01 06 06 01 06 13 01 06 20 01 06 27 01 6: 43 6: 24 6: 56 7: 49 7: 20 7: 19 6: 43 7: 45 6: 45 7: 09 7~ 46 7: 59 7: 20 7: 08 6: 43 6: 24 6: 56 7: 49 7: 21 6~ 43 7: 45 7: 54 7: lP 6: 45 7: 31 7: 34 7: Ol 6: 44 6: 31 ______ P P P P P P P P P P P P P P P P P P P P P P P P P P P P P ___ NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS PRIMARY NECROPSY (WEEK 13) NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS PRIMARY NECROPSY (WEEK 13) OPACITY LEFT EYE OPACITY LEFT EYE OPACITY LEFT EYE NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS GRADE CODE: 1  SLIGHT 2  MODERATE 3  SEVERE P  PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL CLINICAL OBSERVATIONS PAGE 21 TABLE RANGE: 05 31 01 TO 08 31 01 ANIMAL SEX GROUP CATEGORY DATE TIME GRADE OBSERVATIONS 69354 F 0 PPM NORMAL 07 04 01 6: 09 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 24 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 31 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 6: 59 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 6: 51 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 25 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 23 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 28 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 31 01 7: 09 P NO SIGNIFICANT CLINICAL OBSERVATIONS 69354 F 0 PPM DISPOSITION 08 31 01 7: 46 P PRIMARY NECROPSY (WEEK 13) 69366 F 0 PPM NORMAL 06 06 01 7: 35 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: Ol P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 6: 44 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 31 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 09 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 31 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 6: 59 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 6: 51 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 26 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 24 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 28 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 31 01 7: lO P NO SIGNIFICANT CLINICAL OBSERVATIONS 69366 F 0 PPM DISPOSITION 08 31 01 7: 46 P PRIMARY NECROPSY (WEEK 13) 69366 F 0 PPM SPECIAL 07 11 01 6: 25 P WET YELLOW MATERIAL UROGENITAL AREA 69367 F 0 PPM NORMAL 06 06 01 7: 35 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 09 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 25 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 6: 59 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 26 P NO SIGNIFICANT CLINICAL OBSERVATIONS __________________________________

GRADE CODE: 1  SLIGHT 2  MODERATE 3  SEVERE P  PRESENT PROJECT NO.: WIL 417002 DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 22 SPONSOR: HAP TASK FORCE INDIVIDUAL CLINICAL OBSERVATIONS TABLE RANGE: 05 31 01 TO 08 31 01 _______________________________________ ANIMAL SEX GROUP CATEGORY DATE TIME GRADE OBSERVATIONS 69367 F 0 PPM 69367 F 0 PPM 69367 F 0 PPM 69367 F 0 PPM 69378 F 0 PPM CA G 69378 F 0 PPM 69378 F 0 PPM 69378 F 0 PPM 69384 F 0 PPM _______________________ GRADE CODE: 1  SLIGHT 2 NORMAL DISPOSITION EYES/ EARS/ NOSE SPECIAL NORMAL DISPOSITION EYES/ EARS/ NOSE SPECIAL NORMAL 08 15 01 7: 24 P 08 30 01 7: 55 P 08 01 01 6: 53 P 08 22 01 6: 29 P 08 30 01 7: 31 P 06 13 01 7: 02 P 06 20 01 6: 44 P 06 27 01 6: 32 P 07 18 01 7: 32 P 08 01 01 6: 53 P 08 22 01 6: 29 P 06 06 01 7: 35 P 06 13 01 7: 02 P 06 20 01 6: 45 P 06 27 01 6: 32 P 07 04 01 6: lO P 07 11 01 6: 26 P 07 18 01 7: 32 P 07 25 01 7: 00 P 08 08 01 6: 26 P 08 15 01 7~ 25 P 08 31 01 7: 46 P 08 01 01 6: 53 P 08 22 01 6: 29 P 08 31 01 7: lO P 06 06 01 7: 36 P 06 13 01 7: 02 P 06 20 01 6: 45 P 06 27 01 6: 32 P __________________ NO SIGNIFICANT CLINICAL OBSERVATIONS PRIMARY NECROPSY (WEEK 13) OPACITY RIGHT EYE OPACITY RIGHT EYE OPACITY RIGHT EYE WET YELLOW MATERIAL UROGENITAL AREA DRIED YELLOW MATERIAL UROGENITAL AREA WET YELLOW MATERIAL UROGENITAL AREA WET YELLOW MATERIAL UROGENITAL AREA DRIED YELLOW MATERIAL UROGENITAL AREA WET YELLOW MATERIAL UROGENITAL AREA NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS PRIMARY NECROPSY (WEEK 13) RED DISCHARGE RIGHT EYE WET YELLOW MATERIAL UROGENITAL AREA DRIED YELLOW MATERIAL UROGENITAL AREA NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS MODERATE 3  SEVERE P  PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL CLINICAL OBSERVATIONS PAGE 23 TABLE RANGE: 05 31 01 TO 08 31 01 ______________________________________~~~ ANIMAL SEX GROUP CATEGORY DATE TIME GRADE OBSERVATIONS ___________________________________

69384 F 69384 F 69384 F 69384 F 69398 F OH, 69398 F 69398 F CT\

t;

69404 F GRADE CODE: 1 0 PPM NORMAL 0 PPM DISPOSITION 0 PPM EYES/ EARS/ NOSE 0 PPM SPECIAL 0 PPM NORMAL 0 PPM 0 PPM DISPOSITION SPECIAL 0 PPM NORMAL 07 04 01 6: lO P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 26 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 32 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: oo P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6~ 27 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 2S P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 37 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 57 P PRIMARY NEcROPSY (WEEK 13) 08 01 01 6: 56 P PUPIL DILATED RIGHT EYE 08 22 01 6: 30 P WET YELLOW MATERIAL UROGENITAL AREA 06 06 01 7: 36 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 03 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7~ 32 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: oo P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 6: 56 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 27 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7~ 26 P No SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 37 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 57 P PRIMARY NECROPSY (WEEK 13) 06 20 01 6~ 45 P WET YELLOW MATERIAL UROGENITAL AREA 06 27 01 6~ 32 P WET YELLOW MATERIAL UROGENITAL AREA 07 04 01 6: lO P DRIED YELLOW MATERIAL UROGENITAL AREA 07 11 01 6: 28 P DRIED YELLOW MATERIAL UROGENITAL AREA 08 22 01 6: 30 P WET YELLOW MATERIAL UROGENITAL AREA 06 06 01 7: 36 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 03 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 6: 46 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 32 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: lO P NO SIGNIFICANT CLINICAL OBSERVATIONS ______________________________________ SLIGHT 2  MODERATE 3  SEVERE P  PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE ________________________ ANIMAL SEX GROUP ___________________ 69404 F 69404 F 69416 F R ch 69416 F 0, 69416 F UY 6 0 PPM 0 PPM 69419 F 0 PPM _____________ GRADE CODE: 1 0 PPM 0 PPM 0 PPM __ _____  SLIGHT 2 DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS PO DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL CLINICAL OBSERVATIONS PAGE 24 TABLE RANGE: 05 31 01 TO 08 31 01 ________________________________________ CATEGORY DATE TIME GRADE OBSERVATIONS NORMAL 07 11 01 6: 28 P 07 18 01 7: 33 P 07 25 01 7: Ol P 08 01 01 6~ 57 P 08 08 01 6: 27 P 08 15 01 7: 26 P 08 22 01 6: 31 P 08 31 01 7: lO P 08 31 01 7: 46 P 06 06 01 7: 37 P 06 13 01 7: 03 P 06 27 01 6: 33 P 07 04 01 6: lO P 07 11 01 6: 29 P 07 18 01 7: 33 P 07 25 01 7: Ol P 08 08 01 6: 27 P 08 30 01 7: 55 P 06 20 01 6~ 46 P 08 01 01 6: 57 P 08 15 01 7~ 26 P 08 22 01 6: 31 P 08 30 01 7: 33 P 06 06 01 7: 37 P 06 13 01 7: 03 P 06 20 01 6: 47 P 06 27 01 6: 33 P 07 04 01 6: ll P 07 11 01 6: 29 P _____________________ SEVERE P  PRESENT NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS PRIMARY NECROPSY (WEEK 13) NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS PRIMARY NECROPSY (WEEK 13) WET YELLOW MATERIAL UROGENITAL AREA WET YELLOW MATERIAL UROGENITAL AREA WET YELLOW MATERIAL UROGENITAL AREA WET YELLOW MATERIAL UROGENITAL AREA WET YELLOW MATERIAL UROGENITAL AREA NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS NO SIGNIFICANT CLINICAL OBSERVATIONS DISPOSITION NORMAL DISPOSITION SPECIAL NORMAL MODERATE 3 ___________________ DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS PROJECT NO.: WIL 417002 PO DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 25 SPONSOR: HAP TASK FORCE INDIVIDUAL CLINICAL OBSERVATIONS TABLE RANGE: 05 31 01 TO 08 31 01 _______ ANIMAL SEX GROUP CATEGORY DATE TIME GRADE OBSERVATIONS _________ 69419 F 0 PPM 69419 F 0 PPM 69419 F 0 PPM 69419 F 0 PPM 69432 F 0 PPM % 69432 F 0 PPM o\ 69432 F 0 PPM t; 69432 F 0 PPM 69362 F 15 PPM ____________________ GRADE CODE: 1  SLIGHT 2 NORMAL DISPOSITION EYES/ EARS/ NOSE SPECIAL NORMAL DISPOSITION EYES/ EARS/ NOSE SPECIAL NORMAL 07 18 01 7: 33 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: 03 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 6: 57 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 33 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 56 P PRIMARY NECROPSY (WEEK 13) 08 08 01 6: 28 P DRIED RED MATERIAL AROUND LEFT EYE 08 15 01 7: 27 P WET YELLOW MATERIAL UROGENITAL AREA 08 22 01 6: 31 P WET YELLOW MATERIAL UROGENITAL AREA 06 06 01 7: 37 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 04 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 33 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: ll P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 30 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 33 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: 04 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 6: 58 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 28 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 22 01 6: 32 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 31 01 7: lO P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 31 01 7~ 46 P PRIMARY NECR~ PSY (WEEK 13) . 08 15 01 7~ 27 P DRIED RED MATERIAL AROUND LEFT EYE 06 20 01 6~ 47 P WET YELLOW MATERIAL UROGENITAL AREA 08 15 01 7~ 27 P WET YELLOW MATERIAL UROGENITAL AREA 06 06 01 7: 43 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 07 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 6: 52 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 35 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 14 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 36 P NO SIGNIFICANT CLINICAL OBSERVATIONS __________ MODERATE 3  SEVERE P  PRESENT DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS PROJECT NO.: WIL 417002 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE PAGE 26 SPONSOR: HAP TASK FORCE INDIVIDUAL CLINICAL OBSERVATIONS TABLE RANGE: 05 31 01 TO 08 31 01 _____________________________________ ANIMAL SEX GROUP CATEGORY DATE TIME GRADE OBSERVATIONS ______ 69362 F 15 PPM NORMAL 69362 69362 69376 P s 69376 69376 R 69376 o\

;;

69377 F 15 PPM NORMAL 69377 F 15 PPM DISPOSITION 69377 F 15 PPM BEHAVIOR/ CNS F F F F F F 15 PPM DISPOSITION 15 PPM SPECIAL 15 PPM NORMAL 15 PPM DISPOSITION 15 PPM EYES/ EARS/ NOSE 15 PPM SPECIAL 07 18 01 7: 37 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: 07 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 7: 02 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 32 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 32 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 31 01 7: 46 P PRIMARY NECROPSY (WEEK 13) 08 22 01 6: 36 P WET YELLOW MATERIAL UROGENITAL AREA 08 31 01 7: lO P DRIED YELLOW MATERIAL UROGENITAL AREA 06 13 01 7: 08 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 6: 52 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 36 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 14 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6: 36 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7~ 37 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: 08 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 7: 02 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 30 01 7: 51 P PRIMARY NECROPSY (WEEK 13) 08 30 01 7: 36 P DRIED RED MATERIAL AROUND LEFT EYE 08 30 01 7: 36 P RED DISCHARGE LEFT EYE 06 06 01 7: 44 P WET YELLOW MATERIAL UROGENITAL AREA 08 08 01 6: 32 P WET YELLOW MATERIAL UROGENITAL AREA 08 15 01 7: 33 P WET YELLOW MATERIAL UROGENITAL AREA 08 22 01 6: 36 P WET YELLOW MATERIAL UROGENITAL AREA 06 06 01 7: 44 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 08 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 6: 52 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 36 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 30 01 13: 57 P EUTHANIZED IN EXTREMIS DUE PHYSICAL CONDITION 06 30 01 13: 56 P IMPAIRED USE OF LEFT HINDLIMB _________________~~~~ GRADE CODE: 1  SLIGHT 2  MODERATE 3  SEVERE P  PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL CLINICAL OBSERVATIONS TABLE RANGE: 05 31 01 TO 08 31 01 PAGE 27 ANIMAL SEX GROUP ________

69317 F 15 PPM 69377 F 15 PPM 69377 F 15 PPM EXCRETA 69377 F 15 PPM SPECIAL 69382 F 15 PPM NORMAL P 2 0 z 69382 F 15 PPM 5, 69382 F 15 PPM 69390 F 15 PPM ________________ GRADE CODE: 1  SLIGHT 2 CATEGORY BEHAVIOR/ CNS EYES/ EARS/ NOSE DISPOSITION SPECIAL NORMAL __ DATE TIME GRADE OBSERVATIONS ________

06 30 01 13: 56 P IMPAIRED USE OF RIGHT HINDLIMB 06 30 01 13: 52 P DRIED RED MATERIAL AROUND RIGHT EYE 06 30 01 13: 52 P DRIED RED MATERIAL AROUND LEFT EYE 06 30 01 13: 53 P RED DISCHARGE RIGHT EYE 06 30 01 13: 54 P RED DISCHARGE LEFT EYE 06 30 01 13: 54 P DECREASED DEFECATION 06 30 01 13: 55 P WET YELLOW MATERIAL DORSAL TRUNK 06 30 01 13: 55 P WET YELLOW MATERIAL UROGENITAL AREA 06 06 01 7: 45 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 08 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 6: 53 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 27 01 6: 36 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 14 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 11 01 6~ 37 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7~ 37 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 25 01 7: 08 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 7: 02 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 33 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 15 01 7: 33 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 31 01 7: lO P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 31 01 7: 46 p PRIMARY NECROPSY (WEEK 13) 08 22 01 6: 37 P WET YELLOW MATERIAL UROGENITAL AREA 06 06 01 7: 45 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 13 01 7: 09 P NO SIGNIFICANT CLINICAL OBSERVATIONS 06 20 01 6: 53 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 04 01 6: 15 P NO SIGNIFICANT CLINICAL OBSERVATIONS 07 18 01 7: 37 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 01 01 7: 03 P NO SIGNIFICANT CLINICAL OBSERVATIONS 08 08 01 6: 33 P NO SIGNIFICANT CLINICAL OBSERVATIONS  MODERATE 3  SEVERE P  PRESENT PROJECT NO.: WIL 417002 SPONSOR: HAP TASK FORCE DETAILED PHYSICAL EXAMINATIONS/ DISPOSITIONS 90 DAY INHAL. STUDY OF 1,1,2 TCE IN RATS WITH PK IN RATS & MICE INDIVIDUAL CLINICAL OBSERVATIONS PAGE 28 TABLE RANGE: 05 31 01 TO 08 31 01 _____________________________________