3 mL of the desired solvent (methylene chloride for GPC and HPLC, or
hexane for the other cleanups) and continue concentration to
approximately 100 µL.  Repeat the addition of solvent and concentrate
once more.

12.7.5	If the extract is to be cleaned up by GPC, adjust the volume of
the extract to 5.0 mL with methylene chloride.  If the extract is to be
cleaned up by HPLC, concentrate the extract to 1.0 mL.  Proceed with GPC
or HPLC cleanup (Section 13.2 or 13.5, respectively).

12.7.6	If the extract is to be cleaned up by column chromatography
(silica gel, Carbopak/Celite, or Florisil), bring the final volume to
1.0 mL with hexane.  Proceed with column cleanup (Sections 13.3, 13.4,
or 13.7).

12.7.7	If the extract is to be concentrated for injection into the GC/MS
(Section 14), quantitatively transfer the extract to a 0.3-mL conical
vial for final concentration, rinsing the larger vial with hexane and
adding the rinse to the conical vial.  Reduce the volume to
approximately 100 µL.  Add 20 µL of nonane to the vial, and evaporate
the solvent to the level of the nonane.  Seal the vial and label with
the sample number.  Store in the dark at room temperature until ready
for GC/MS analysis.  If GC/MS analysis will not be performed on the same
day, store the vial at less than -10 ºC.

13.0	Extract cleanup

13.1	Cleanup may not be necessary for relatively clean samples (e.g.,
treated effluents, groundwater, drinking water).  If particular
circumstances require the use of a cleanup procedure, the laboratory may
use any or all of the procedures below or any other appropriate
procedure.  Before using a cleanup procedure, the laboratory must
demonstrate that the requirements of Section 9.2 can be met using the
cleanup procedure.

13.1.1	Gel permeation chromatography (Section 13.2) removes high
molecular weight interferences that cause GC column performance to
degrade.  It should be used for all soil and sediment extracts.  It may
be used for water extracts that are expected to contain high molecular
weight organic compounds (e.g., polymeric materials, humic acids).  It
should also be used for tissue extracts after initial cleanup on the
anthropogenic isolation column (Section 13.6).

13.1.2	Acid, neutral, and basic silica gel (Section 13.3) and Florisil
(Section 13.7) are used to remove non-polar and polar interferences.

13.1.3	Carbopak/Celite (Section 13.4) can be used to separate CBs 77,
126, and 169 from the mono- and di- ortho-substituted CBs, if desired.

13.1.4	HPLC (Section 13.5) is used to provide specificity for certain
congeners and congener groups.

13.1.5	The anthropogenic isolation column (Section 13.6) is used for
removal of lipids from tissue samples.

13.2	Gel permeation chromatography (GPC)

13.2.1	Column packing

<IP_ADDRESS>	Place 70 to 75 g of SX-3 Bio-beads (Section <IP_ADDRESS>) in a 400-
to 500-mL beaker.

<IP_ADDRESS>	Cover the beads with methylene chloride and allow to swell
overnight (a minimum of 12 hours).

<IP_ADDRESS>	Transfer the swelled beads to the column (Section <IP_ADDRESS>) and
pump solvent through the column, from bottom to top, at 4.5 to 5.5
mL/minute prior to connecting the column to the detector.

<IP_ADDRESS>	After purging the column with solvent for 1 to 2 hours, adjust
the column head pressure to 7 to 10 psig and purge for 4 to 5 hours to
remove air.  Maintain a head pressure of 7 to 10 psig.  Connect the
column to the detector (Section <IP_ADDRESS>).

13.2.2	Column calibration

<IP_ADDRESS>	Load 5 mL of the GPC calibration solution (Section 7.4) into
the sample loop.

<IP_ADDRESS>	Inject the GPC calibration solution and record the signal from
the detector.  The elution pattern will be corn oil, BEHP, methoxychlor,
perylene, and sulfur.

<IP_ADDRESS>	Set the “dump time” to allow >85% removal of BEHP and >85%
collection of methoxychlor.

<IP_ADDRESS>	Set the “collect time” to the time of the sulfur peak
maximum.

<IP_ADDRESS>	Verify calibration with the GPC calibration solution after
every 20 extracts.  Calibration is verified if the recovery of the
methoxychlor is greater than 85%.  If calibration is not verified, the
system must be recalibrated using the GPC calibration solution, and the
previous sample batch must be re-extracted and cleaned up using the
calibrated GPC system.

13.2.3	Extract cleanup – GPC requires that the column not be
overloaded.  The column specified in this Method is designed to handle a
maximum of 0.5 g of material from an aqueous, soil, or mixed-phase
sample in a 5-mL extract, and has been shown to handle 1.5 g of lipid
from a tissue sample in a 5-mL extract.  If the extract is known or
expected to contain more than these amounts, the extract is split into
aliquots for GPC, and the aliquots are combined after elution from the
column.  The residue content of the extract may be obtained
gravimetrically by evaporating the solvent from a 50-µL aliquot.

<IP_ADDRESS>	Filter the extract or load through the filter holder (Section
<IP_ADDRESS>) to remove particles.  Load the 5.0-mL extract onto the column.

<IP_ADDRESS>	Elute the extract using the calibration data determined in
Section 13.2.2.  Collect the eluate in a clean 400- to 500-mL beaker.
Allow the system to rinse for additional 10 minutes before injecting the
next sample.

<IP_ADDRESS>	Rinse the sample loading tube thoroughly with methylene
chloride between extracts to prepare for the next sample.

<IP_ADDRESS>	If an extract is encountered that could overload the GPC column
to the extent that carry-over could occur, a 5.0-mL methylene chloride
blank must be run through the system to check for carry-over.

<IP_ADDRESS>	Concentrate the eluate per Sections 12.6 and 12.7 for further
cleanup or injection into the GC/MS.

13.3	Silica gel cleanup

13.3.1	Place a glass-wool plug in a 15-mm ID chromatography column
(Section  <IP_ADDRESS>).  Pack the column bottom to top with: 1 g silica gel
(Section <IP_ADDRESS>), 4 g basic silica gel (Section <IP_ADDRESS>), 1 g silica
gel, 8 g acid silica gel (Section <IP_ADDRESS>), 2 g silica gel, and 4 g
granular anhydrous sodium sulfate (Section 7.2.1).  Tap the column to
settle the adsorbents.

13.3.2	Pre-elute the column with 50 to 100 mL of hexane.  Close the
stopcock when the hexane is within 1 mm of the sodium sulfate.  Discard
the eluate.  Check the column for channeling.  If channeling is present,
discard the column and prepare another.

13.3.3	Apply the concentrated extract to the column.  Open the stopcock
until the extract is within 1 mm of the sodium sulfate.

13.3.4	Rinse the receiver twice with 1-mL portions of hexane, and apply
separately to the column.  Elute the CBs with 25 mL of hexane and
collect the eluate.

13.3.5	Concentrate the eluate per Section 12.6 and 12.7 for further
cleanup or injection into the HPLC or GC/MS.

13.3.6	For extracts of samples known to contain large quantities of
other organic compounds, it may be advisable to increase the capacity of
the silica gel column.  This may be accomplished by increasing the
strengths of the acid and basic silica gels.  The acid silica gel
(Section <IP_ADDRESS>) may be increased in strength to as much as 40% w/w
(6.7 g sulfuric acid added to 10 g silica gel).  The basic silica gel
(Section <IP_ADDRESS>) may be increased in strength to as much as 33% w/w (50
mL 1N NaOH added to 100 g silica gel), or the potassium silicate
(Section <IP_ADDRESS>) may be used.

Note:	The use of stronger acid silica gel (44% w/w) may lead to charring
of organic compounds in some extracts.  The charred material may retain
some of the analytes and lead to lower recoveries of the CBs.
Increasing the strengths of the acid and basic silica gel may also
require different volumes of hexane than those specified above to elute
the analytes from the column.  The performance of the Method after such
modifications must be verified by the procedure in Section 9.2.

 ADVANCE \d7

13.4	Carbon column (Reference 16)

13.4.1	Cut both ends from a 50-mL disposable serological pipet (Section
<IP_ADDRESS>) to produce a 20-cm column.  Fire-polish both ends and flare
both ends if desired.  Insert a glass-wool plug at one end, and pack the
column with 3.6 g of Carbopak/Celite (Section <IP_ADDRESS>) to form an
adsorbent bed 20 cm long.  Insert a glass-wool plug on top of the bed to
hold the adsorbent in place.

13.4.2	Pre-elute the column with 20 mL each in succession of toluene,
methylene chloride, and hexane.

13.4.3	When the solvent is within 1 mm of the column packing, apply the
n-hexane sample extract to the column.  Rinse the sample container twice
with 1-mL portions of hexane and apply separately to the column.  Apply
2 mL of hexane to complete the transfer.

13.4.4	Elute the column with 25 mL of n-hexane and collect the eluate.
This fraction will contain the mono- and di-ortho CBs.  If carbon
particles are present in the eluate, filter through glass-fiber filter
paper.

13.4.5	Elute the column with 15 mL of methanol and discard the eluate.
The fraction discarded will contain residual lipids and other potential
interferents, if present.

13.4.6	Elute the column with 15 mL of toluene and collect the eluate.
This fraction will contain CBs 77, 126, and 169.  If carbon particles
are present in the eluate, filter through glass-fiber filter paper.

13.4.7	Concentrate the fractions per Section 12.6 and 12.7 for further
cleanup or injection into the HPLC or GC/MS.

13.5	HPLC (References 4 and 17)

13.5.1	Column calibration

<IP_ADDRESS>	Prepare a calibration standard containing the Toxics and other
congeners of interest at the concentrations of the stock solution in
Table 3, or at a concentration appropriate to the response of the
detector.

<IP_ADDRESS>	Inject the calibration standard into the HPLC and record the
signal from the detector.  Collect the eluant for reuse.  Elution will
be in the order of the di-ortho, mono-ortho, and non-ortho congeners.

<IP_ADDRESS>	Establish the collection time for the congeners of interest.
Following calibration, flush the injection system with solvent to ensure
that residual CBs are removed from the system.

<IP_ADDRESS>	Verify the calibration with the calibration solution after
every 20 extracts.  Calibration is verified if the recovery of the CBs
is 75 to 125% compared to the calibration (Section <IP_ADDRESS>).  If
calibration is not verified, the system must be recalibrated using the
calibration solution, and the previous 20 samples must be re-extracted
and cleaned up using the calibrated system.

13.5.2	Extract cleanup – HPLC requires that the column not be
overloaded.  The column specified in this Method is designed to handle a
maximum of 5-50 µg of a given CB, depending on the congener (Reference
17).  If the amount of material in the extract will overload the column,
split the extract into fractions  and combine the fractions after
elution from the column.

<IP_ADDRESS>	Rinse the sides of the vial containing the sample and adjust to
the volume required for the sample loop for injection.

<IP_ADDRESS>	Inject the sample extract into the HPLC.

<IP_ADDRESS>	Elute the extract using the calibration data determined in
Section 13.5.1.  Collect the fraction(s) in clean 20-mL concentrator
tubes.

<IP_ADDRESS>	If an extract containing greater than 500 µg of total CBs is
encountered, a blank must be run through the system to check for
carry-over.

<IP_ADDRESS>	Concentrate the eluate per Section 12.7 for injection into the
GC/MS.

13.6	Anthropogenic isolation column (Reference 3) – Used for removal
of lipids from tissue extracts

13.6.1	Prepare the column as given in Section 7.5.3.

13.6.2	Pre-elute the column with 100 mL of hexane.  Drain the hexane
layer to the top of the column, but do not expose the sodium sulfate.

13.6.3	Load the sample and rinses (Section <IP_ADDRESS>) onto the column by
draining each portion to the top of the bed.  Elute the CBs from the
column into the apparatus used for concentration (Section 12.4.7) using
200 mL of hexane.

13.6.4	Remove a small portion (e.g., 50 µL) of the extract for
determination of residue content.  Estimate the percent of the total
that this portion represents.  Concentrate the small portion to constant
weight per Section <IP_ADDRESS>.  Calculate the total amount of residue in
the extract.  If more than 500 mg of material remains, repeat the
cleanup using a fresh anthropogenic isolation column.

13.6.5	If necessary, exchange the extract to a solvent suitable for the
additional cleanups to be used (Section 13.2-13.5 and 13.7).

13.6.6	Clean up the extract using the procedures in Sections 13.2-13.5
and 13.7.  GPC (Section 13.2) and Florisil (Section 13.7) are
recommended as minimum additional cleanup steps.

13.6.7	Following cleanup, concentrate the extract to 20 µL as described
in Section 12.7 and proceed with the analysis in Section 14.

13.7	Florisil cleanup (Reference 18)

13.7.1	Begin to drain the n-hexane from the column (Section <IP_ADDRESS>.2).
Adjust the flow rate of eluant to 4.5-5.0 mL/min.

13.7.2	When the n-hexane is within 1 mm of the sodium sulfate, apply the
sample extract (in hexane) to the column.  Rinse the sample container
twice with 1-mL portions of hexane and apply to the column.

13.7.3	Elute the mono-ortho and di-ortho CBs with approx 165 mL of
n-hexane and collect the eluate.  Elute the non-ortho co-planar CBs with
approx 100 mL of 6% ether:hexane and collect the eluate.  The exact
volumes of solvents will need to be determined for each batch of
Florisil.  If the mono/di-ortho CBs are not to be separated from the
non-ortho co-planar CBs, elute all CBs with 6% ether:hexane.

13.7.4	Concentrate the eluate(s) per Sections 12.6-12.7 for further
cleanup or for injection into the HPLC or GC/MS.

14.0	HRGC/HRMS analysis

14.1	Establish the operating conditions given in Section 10.1.

14.2	Add 2 µL of the labeled injection internal standard spiking
solution (Section 7.14) to the 20 µL sample extract immediately prior
to injection to minimize the possibility of loss by evaporation,
adsorption, or reaction.  If an extract is to be reanalyzed and
evaporation has occurred, do not add more labeled injection internal
standard spiking solution.  Rather, bring the extract back to its
previous volume (e.g., 19 µL) with pure nonane (18 µL if 2 µL
injections are used).

14.3	Inject 1.0 or 2.0 µL of the concentrated extract containing the
Labeled injection internal standards using on-column or splitless
injection.  The volume injected must be identical to the volume used for
calibration (Section 10.3).

14.3.1	Start the GC column initial isothermal hold upon injection.
Start MS data collection after the solvent peak elutes.

14.3.2	Monitor the exact m/z’s at each LOC throughout the LOC
retention time window.  Where warranted, monitor m/z’s associated with
congeners at higher levels of chlorination to assure that fragments are
not interfering with the m/z’s  for congeners at lower levels of
chlorination.  Also where warranted, monitor  m/z’s associated with
interferents expected to be present.

14.3.3	Stop data collection after 13C12-DeCB has eluted.  Return the
column to the initial temperature for analysis of the next extract or
standard.

15.0	System and laboratory performance

15.1	At the beginning of each 12-hour shift during which analyses are
performed, GC/MS system performance and calibration are verified for all
native CBs and labeled compounds.  For these tests, analyze the diluted
combined 209 congener solution (Section <IP_ADDRESS>) to verify all
performance criteria.  Adjustment and/or recalibration (Section 10) must
be performed until all performance criteria are met.  Only after all
performance criteria are met may samples, blanks, IPRs, and OPRs be
analyzed.

15.2	MS resolution – Static resolving power checks must be performed
at the beginning and at the end of each shift per Section 10.2.1.  If
analyses are performed on successive shifts, only the beginning of shift
static resolving power check is required.  If the requirement in Section
10.2.1 cannot be met, the problem must be corrected before analyses can
proceed.  If any of the samples in the previous shift may be affected by
poor resolution, those samples must be re-analyzed.

15.3	Calibration verification

15.3.1	Inject and analyze the Diluted combined 209 congener solution
(Section <IP_ADDRESS>.2) using the procedure in Section 14.

15.3.2	The m/z abundance ratios for each native CB and labeled compound
in the VER standard must be within the limits in Table 8; otherwise, the
mass spectrometer must be adjusted until the m/z abundance ratios fall
within the limits specified when the verification test is be repeated.
If the adjustment alters the resolution of the mass spectrometer,
resolution must be verified (Section 10.2.1) prior to repeat of the
verification test.

15.3.3	The GC peak representing each native CB and labeled compound in
the VER standard must be present with a S/N of at least 10; otherwise,
the mass spectrometer must be adjusted and the verification test
repeated.

15.3.4	Compute the recovery of the Toxics/LOC CBs by isotope dilution
(Section 17.1) and the labeled compounds by internal standard (17.2).
These recoveries are computed based on the calibration data in Section
10.

15.3.5	For each compound, compare the recovery with the calibration
verification limit in Table 6.  If all compounds meet the acceptance
criteria, calibration has been verified and analysis of standards and
sample extracts may proceed.  If, however, any compound fails its
respective limit, the measurement system is not performing properly.  In
this event, prepare a fresh calibration standard or correct the problem
and repeat the resolution (Section 15.2) and verification (Section 15.3)
tests, or recalibrate (Section 10).  If recalibration is required,
recalibration for the 209 congeners (Section 10.5) must also be
performed.

15.4	Retention times and GC resolution

15.4.1	Retention times

<IP_ADDRESS>	Absolute – The absolute retention times of the Labeled
Toxics/LOC/window defining standard congeners (Section 7.12) in the
verification test (Section 15.3) must be within ± 15 seconds of the
respective retention times in the calibration or, if an alternate column
or column system is employed, within ± 15 seconds of the respective
retention times in the calibration for the alternate column or column
system (Section <IP_ADDRESS>).

<IP_ADDRESS>	Relative – The relative retention times of native CBs and
labeled compounds in the verification test (Section 15.3) must be within
their respective RRT limits in Table 2 or, if an alternate column or
column system is employed, within their respective RRT limits for the
alternate column or column system (Section <IP_ADDRESS>).

<IP_ADDRESS>	If the absolute or relative retention time of any compound is
not within the limits specified, the GC is not performing properly.  In
this event, adjust the GC and repeat the verification test (Section
15.3) or recalibrate (Section 10), or replace the GC column and either
verify calibration or recalibrate.

15.4.2	GC resolution and minimum analysis time

<IP_ADDRESS>	As a final step in calibration verification, GC resolution and
minimum analysis time are verified and response factors for congeners
other than the Toxics and LOC CBs are updated.

<IP_ADDRESS>	The resolution and minimum analysis time specifications in
Sections <IP_ADDRESS>.2 and <IP_ADDRESS>.1, respectively, must be met for the
SPB-octyl column or, if an alternate column or column system is
employed, must be met as specified for the alternate column or column
system (Section <IP_ADDRESS>).  If these specifications are not met, the GC
analysis conditions must be adjusted until the specifications are met,
or the column must be replaced and the calibration verification tests
repeated Sections 15.4.1 through <IP_ADDRESS>), or the system must be
recalibrated (Section 10).

<IP_ADDRESS>	After the resolution and minimum analysis time specifications
are met, update the retention times and relative retention times for all
congeners, and response factors for all congeners except the Toxics and
LOC CBs.  For the Toxics and LOC CBs, the multi-point calibration data
must be used ( Section 10.4) and verified (Section 15.3.4).

15.5	Ongoing precision and recovery

15.5.1	Analyze the extract of the ongoing precision and recovery (OPR)
aliquot (Section <IP_ADDRESS>, 11.5.4, 11.6.2, or <IP_ADDRESS>) prior to
analysis of samples from the same batch.

15.5.2	Compute the percent recovery of the Toxics/LOC CBs by isotope
dilution  (Section 10.4).  Compute the percent recovery of each labeled
compound by the internal standard method (Section 10.5).

15.5.3	For the Toxics/LOC CBs and labeled compounds, compare the
recovery to the OPR limits given in Table 6.  If all compounds meet the
acceptance criteria, system performance is acceptable and analysis of
blanks and samples may proceed.  If, however, any individual
concentration falls outside of the range  given, the
extraction/concentration processes are not being performed properly for
that compound.  In this event, correct the problem, re-prepare, extract,
and clean up the sample batch and repeat the ongoing precision and
recovery test (Section 15.5).

15.5.4	If desired, add results that pass the specifications in Section
15.5.3 to initial and previous ongoing data for each compound in each
matrix.  Update QC charts to form a graphic representation of continued
laboratory performance.  Develop a statement of laboratory accuracy for
each congener in each matrix type by calculating the average percent
recovery (R) and the standard deviation of percent recovery (SR).
Express the accuracy as a recovery interval from R - 2SR to R + 2SR.
For example, if R = 95% and SR = 5%, the accuracy is 85 to 105%.

15.6	Blank – Analyze the Method blank extracted with each sample batch
immediately following analysis of the OPR aliquot to demonstrate freedom
from contamination and freedom from carryover from the OPR analysis. If
CBs will be carried from the OPR into the Method blank, analyze one or
more aliquots of solvent between the OPR and the Method blank.  The
results of the analysis of the blank must meet the specifications in
Section 9.5.2 before sample analyses may proceed.

16.0	Qualitative determination

A CB or labeled compound is identified in a standard, blank, or sample
when all of the criteria in Sections 16.1 through 16.4 are met.

16.1	The signals for the two exact m/z’s in Table 7 must be present
and must maximize within the same two scans.

16.2	The signal-to-noise ratio (S/N) for the GC peak at each exact m/z
must be greater than or equal to 2.5 for each CB detected in a sample
extract, and greater than or equal to 10 for all CBs in the calibration
and verification standards (Sections 10.3.3 and 15.3.3).

Note:	  An interference between DiCB m/z 223.9974 and PFK m/z 223.9872
may preclude meeting the S/N requirement for the DiCB congeners.  If
identification is ambiguous, an experienced spectrometrist (Section 1.4)
must determine the presence or absence of the congener. ADVANCE \d7

16.3	The ratio of the integrated areas of the two exact m/z’s
specified in Table 7 must be within the limit in Table 8, or within ±
15 percent of the ratio in the midpoint (CS-3) calibration or
calibration verification (VER), whichever is most recent.

16.4	The relative retention time of the peak for a CB must be within the
RRT QC limits specified in Table 2 or within similar limits developed
from calibration data (Section 10.1.2).   If an alternate column or
column system is employed, the RRT for the CB must be within its
respective RRT QC limits for the alternate column or column system
(Section <IP_ADDRESS>).

Note:	  For native CBs determined by internal standard quantitation, a
given CB congener may fall within more than one RT window and be
mis-identified unless the RRT windows are made very narrow, as in Table
2.  Therefore, consistency of the RT and RRT with other congeners and
the labeled compounds may be required for rigorous congener
identification.  Retention time regression analysis may aid in this
identification. ADVANCE \d7

16.5	Because of congener overlap and the potential for interfering
substances, it is possible that all of the identification criteria
(Sections 16.1-16.4) may not be met.  It is also possible that loss of
one or more chlorines from a highly chlorinated congener may inflate or
produce a false concentration for a less-chlorinated congener that
elutes at the same retention time (see Section 18.5).  If identification
is ambiguous, an experienced spectrometrist (Section 1.4) must determine
the presence or absence of the congener.

16.6	If the criteria for identification in Sections 16.1-16.5 are not
met, the CB has not been identified and the result for that congener may
not be reported or used for permitting or regulatory compliance
purposes.  If interferences preclude identification, a new aliquot of
sample must be extracted, further cleaned up, and analyzed.

17.0	Quantitative determination

17.1	Isotope dilution quantitation

17.1.1	By adding a known amount of the Labeled
Toxics/LOC/window-defining compounds to every sample prior to
extraction, correction for recovery of the CBs can be made because the
native compound and its labeled analog exhibit similar effects upon
extraction, concentration, and gas chromatography.  Relative responses
(RRs) are used in conjunction with the calibration data in Section 10.4
to determine concentrations in the final extract, so long as labeled
compound spiking levels are constant.

17.1.2	Compute the concentrations in the extract of the Native
Toxics/LOC CBs using the RRs from the calibration data (Section 10.4)
and following equation:

	where:

	Cex	=	concentration of the PCB in the extract (ng/mL) and the other
terms are as defined in Section 10.5.1

17.2	Internal standard quantitation and labeled compound recovery

17.2.1	Compute the concentrations in the extract of the labeled
compounds (except labeled CB 178) and of the native compounds other than
those in the Native Toxics/LOC standard using the response factors
determined from calibration (Section 10.5)  or calibration verification
(Section <IP_ADDRESS>) and the following equation:

	where:

	Cex	=	concentration of the native or labeled compound in the extract
(ng/mL) and the other terms are as defined in Section 10.5.1

17.2.2	Using the concentration in the extract determined above, compute
the percent recovery of the Labeled Toxics/LOC/window-defining CBs and
the Labeled cleanup standard CBs using the following equation:

17.3	The concentration of a native CB in the solid phase of the sample
is computed using the concentration of the compound in the extract and
the weight of the solids (Section <IP_ADDRESS>), as follows:

where:

Cex	=	The concentration of the compound in the extract (ng/mL).

Vex	=	The extract volume in mL.

Ws	=	The sample weight (dry weight) in kg.

17.4	The concentration of a native CB in the aqueous phase of the sample
is computed using the concentration of the compound in the extract and
the volume of water extracted (Section <IP_ADDRESS>), as follows:

where:

Cex	=	The concentration of the compound in the extract (pg/mL).

Vex	=	The extract volume in mL.

Vs	=	The sample volume in liters.

17.5	If the SICP area at either quantitation m/z for any congener
exceeds the calibration range of the system, dilute the sample extract
by the factor necessary to bring the concentration within the
calibration range, adjust the concentration of the Labeled injection
internal standard to 100 pg/µL in the extract, and analyze an aliquot
of this diluted extract.  If the CBs cannot be measured reliably by
isotope dilution, dilute and analyze an aqueous sample or analyze a
smaller portion of a soil, tissue, or mixed-phase sample.  Adjust the CB
congener concentrations, detection limits, and minimum levels to account
for the dilution.

17.6	Reporting of results – Results are reported to three significant
figures for the CBs and labeled compounds found in all standards,
blanks, and samples.

17.6.1	 Reporting units and levels

<IP_ADDRESS>	Aqueous samples – Report results in pg/L
(parts-per-quadrillion).

<IP_ADDRESS>	Samples containing greater than 1% solids (soils, sediments,
filter cake, compost) – Report results in ng/kg based on the dry
weight of the sample.  Report the percent solids so that the result may
be converted to aqueous units.

<IP_ADDRESS>	Tissues – Report results in ng/kg of wet tissue, not on the
basis of the lipid content of the tissue.  Report the percent lipid
content, so that the data user can calculate the concentration on a
lipid basis if desired.

<IP_ADDRESS>	Reporting level

<IP_ADDRESS>.1	Report the result for each congener at or above the minimum
level of quantitation (ML; Table 2) for analyses of blanks, standards,
and samples.  The MLs in Table 2 are the levels that can be achieved in
the presence of common laboratory contamination.  A laboratory may
establish an ML for a CB congener lower than the MLs in Table 2.  MLs
may be established as low as the lowest calibration point (Table 5)
provided that the concentration of the congener in a minimum of 10
blanks for a sample medium (e.g., water, soil, sludge, tissue) is
significantly below the ML in Table 2.  “Significant” means that the
ML for the congener is no less than 2 standard deviations above the mean
(average) level in the minimum of 10 blanks (Reference 19).  The blanks
must be analyzed during the same period that samples are analyzed,
ideally over an approximately 1-month period.

<IP_ADDRESS>.2	Standards (VER, IPR, OPR) and samples – Report the result
for each congener at or above the ML (Table 2) to 3 significant figures.
 Report results below the ML as <ML (where ML is the concentration at
the ML) or as required by the regulatory authority or permit.

<IP_ADDRESS>.3	Blanks – Report the result for each congener above the ML
to 3 significant figures.  Report a result below the ML but above the
MDL to 2 significant figures.  Report a result below the MDL as <MDL
(where MDL is the concentration at the MDL) or as required by the
regulatory authority or permit.

<IP_ADDRESS>.4	Blank correction – Blank-corrected results may be reported
in addition to reporting of separate results for samples (Section
<IP_ADDRESS>.1) and blanks (Section <IP_ADDRESS>.2).  The recommended procedure
for blank correction (Reference 19) is that a result is significantly
above the blank level, and the level in the blank may be subtracted, if
the result is 2 standard deviations above the mean (average) of results
of analyses of 10 or more blanks for a sample medium.

17.6.2	Results for a CB in a sample that has been diluted are reported
at the least dilute level at which the area at the quantitation m/z is
within the calibration range (Section 17.5).

17.6.3	For a CB having a labeled analog, report results at the least
dilute level at which the area at the quantitation m/z is within the
calibration range (Section 17.5) and the labeled compound recovery is
within the normal range for the Method  (Section 9.3 and Table 6).

17.6.4	If requested, the total concentration of all congeners at a given
level of  chlorination (homolog; i.e., total TrCB, total PeCB, total
HxCB) may be reported by summing the concentrations of all congeners
identified at that LOC, including both the Toxics and other congeners.
Also if requested, total CBs may be reported by summing all congeners
identified at all LOCs.

17.6.5	Reporting of coeluting PCB congeners–Optionally, Delaware River
Basin Commission (DRBC) data qualifier flags and conventions for
reporting coeluting congeners (see
http://www.state.nj.us/drbc/PCB_info.htm), or other reporting convention
agreed upon between the laboratory and the discharger/permittee or
regulatory/control authority, may be used.

18.0	Analysis of complex samples

18.1	Some samples may contain high levels (>10 ng/L; >1000 ng/kg) of the
compounds of interest, interfering compounds, and/or polymeric
materials.  Some extracts may not concentrate to 20 µL (Section
12.7.7); others may overload the GC column and/or mass spectrometer.
Fragment ions from congeners at higher levels of chlorination may
interfere with determination of congeners at lower levels of
chlorination.

18.2	Analyze a smaller aliquot of the sample (Section 17.5) when the
extract will not concentrate to 20 µL after all cleanup procedures have
been exhausted.  If a smaller aliquot of soils or mixed-phase samples is
analyzed, attempt to assure that the sample is representative.

18.3	Perform integration of peak areas and calculate concentrations
manually when interferences preclude computerized calculations.

18.4	Several laboratories have reported that backgrounds of many of the
CB congeners are difficult to eliminate, and that these backgrounds can
interfere with the determination of the CBs in environmental samples.
Backgrounds of Toxics with congener numbers 105, 114, 118, 123, 156,
157, and 167 are common.  The effects of contamination on results for
these congeners should be understood in order to make a reliable
determination.

18.5	Interferences may pose a problem in the determination of congeners
81, 123, 126, and 169 in  some environmental samples.  Loss of one or
more chlorines from a highly chlorinated congener may inflate or produce
a false concentration for a less-chlorinated congener that elutes at the
same retention time.  If, upon inspection of the chromatogram, the
possibility of interferences is evident (e.g., high concentrations of
fragments from loss of one or two chlorines from higher chlorinated
closely eluting congeners), carbon column fractionation (Section 13.4)
and analysis is recommended.

18.6	Recovery of labeled compounds – In most samples, recoveries of
the labeled compounds will be similar to those from reagent water or
from the alternate matrix (Section 7.6).

18.6.1	If the recovery of any of the labeled compounds is outside of the
normal range (Table 6), a diluted sample must be analyzed (Section
17.5).

18.6.2	If the recovery of any of the labeled compounds in the diluted
sample is outside of normal range, the Diluted combined 209 congener
solution (Section <IP_ADDRESS>.2) must be analyzed and calibration verified
(Section 15.3).

18.6.3	If the calibration cannot be verified, a new calibration must be
performed and the original sample extract reanalyzed.

18.6.4	If calibration is verified and the diluted sample does not meet
the limits for labeled compound recovery, the Method does not apply to
the sample being analyzed and the result may not be reported or used for
permitting or regulatory compliance purposes.  In this case, alternate
extraction and cleanup procedures in this Method or an alternate GC
column must be employed to resolve the interference.  If all cleanup
procedures in this Method and an alternate GC column have been employed
and labeled compound recovery remains outside of the normal range,
extraction and/or cleanup procedures that are beyond this scope of this
Method will be required to analyze the sample.

19.0	Pollution prevention

19.1	Pollution prevention encompasses any technique that reduces or
eliminates the quantity or toxicity of waste at the point of generation.
 Many opportunities for pollution  prevention exist in laboratory
operation.  EPA has established a preferred hierarchy of environmental
management techniques that places pollution prevention as the management
option of first choice.  Whenever feasible, laboratory personnel should
use pollution prevention techniques to address waste generation.  When
wastes cannot be reduced feasibly at the source, the Agency recommends
recycling as the next best option.

19.2	The CBs in this Method are used in extremely small amounts and pose
little threat to the environment when managed properly.  Standards
should be prepared in volumes  consistent with laboratory use to
minimize the disposal of excess volumes of expired standards.

19.3	For information about pollution prevention that may be applied to
laboratories and research institutions, consult Less is Better:
Laboratory Chemical Management for Waste Reduction, available from the
American Chemical Society's Department of Governmental Relations and
Science Policy, 1155 16th Street NW, Washington DC 20036, 202/872-4477.

20.0	Waste management

20.1	The laboratory is responsible for complying with all Federal,
State, and local regulations governing waste management, particularly
the hazardous waste identification rules and land disposal restrictions,
and to protect the air, water, and land by minimizing and controlling
all releases from fume hoods and bench operations.  Compliance is also
required with any sewage discharge permits and regulations.  An overview
of requirements can be found in Environmental Management Guide for Small
Laboratories (EPA 233-B-98-001).

20.2	Samples containing HCl or H2SO4 to pH <2 are hazardous and must be
neutralized before being poured down a drain or must be handled as
hazardous waste.

20.3	The CBs decompose above 800 ºC.  Low-level waste such as absorbent
paper, tissues, animal remains, and plastic gloves may be burned in an
appropriate incinerator.  Gross quantities (milligrams) should be
packaged securely and disposed of through commercial or governmental
channels that are capable of handling extremely toxic wastes.

20.4	Liquid or soluble waste should be dissolved in methanol or ethanol
and irradiated with ultraviolet light with a wavelength shorter than 290
nm for several days.  Use F40 BL or equivalent lamps.  Analyze liquid
wastes, and dispose of the solutions when the CBs can no longer be
detected.

20.5	For further information on waste management, consult The Waste
Management Manual for Laboratory Personnel and Less is Better-Laboratory
Chemical Management for Waste Reduction, available from the American
Chemical Society's Department of Government Relations and Science
Policy, 1155 16th Street NW, Washington, DC 20036.

21.0	Method performance

The original version of Method 1668 was validated in single-laboratory
studies at Pacific Analytical, Inc., Carlsbad, California and AXYS
Analytical Services, Ltd., Sidney, British Columbia, Canada.  The next
version, Method 1668A, was validated and data were collected at AXYS
Analytical (Reference 20).  Method 1668A was subjected to peer review in
1999, and published in 2000.  In 2003-2004, EPA conducted an
interlaboratory method validation study of Method 1668A (Reference 21),
subjected the study to a peer review, and subsequently published
interlaboratory performance data in Method 1668B.

After release of Method 1668B, it was reported to EPA that some of the
QC acceptance criteria in Method 1668B did not allow excursions above
100 percent.  As a result, the QC acceptance criteria were re-developed
using data from the interlaboratory study and data from AXYS Analytical
and TestAmerica-Knoxville, Tennessee.  The revised QC acceptance
criteria were published in addendum to the Interlaboratory Study Report
(Reference 22).

Subsequent to development of the revised QC acceptance criteria, AXYS
Analytical, TestAmerica-Knoxville, and Battelle-Columbus provided method
detection limit (MDL) data to EPA.  These data were combined to produced
pooled MDLs and MLs (Reference 23).  Method 1668B was revised to Method
1668C to incorporate the revised QC acceptance criteria and revised MDLs
and MLs.

Figure 8 is a chromatogram showing method performance at each level of
chlorination.

22.0	References

1.	Van den Berg, Martin, Linda S. Birnbaum, Michael Denison, Mike De
Vito, William Farland, Mark Feeley, Heidelore Fiedler, Helen Hakansson,
Annika Hanberg, Laurie Haws, Martin Rose, Stephen Safe, Dieter Schrenk,
Chiharu Tohyama, Angelika Tritscher, Jouko Tuomisto, Mats Tysklind,
Nigel Walker, and Richard E. Peterson, 2006, “The 2005 World Health
Organization Reevaluation of Human and Mammalian Toxic Equivalency
Factors for Dioxins and Dioxin-like Compounds,”  Toxicological
Sciences 93(2): 223-241.

2.	“Sampling and Analytical Methods of the National Status and Trends
Program Mussel Watch Project: 1993-1996 Update,” NOAA Technical
Memorandum NOS ORCS 130, Coastal Monitoring and Bioeffects Assessment
Division, Office of Ocean Resources Conservation and Assessment,
National Ocean Service, National Oceanic and Atmospheric Administration,
U.S. Department of Commerce, N/ORCA2, SSMC4, 1305 East-West Highway,
Silver Spring, MD 20910, p. 3, 1998.

3.	Kuehl, D.W., B.C. Butterworth, J. Libal, and P. Marquis, “An
Isotope Dilution High Resolution Gas Chromatography-High Resolution Mass
Spectrometric Method for the Determination of Coplanar Polychlorinated
Biphenyls: Application to Fish and Marine Mammals,” Chemosphere
22:9-10, 849-858, 1991.

4.	Echols, Kathy, Robert Gale, Donald E. Tillitt, Ted Schwartz, and
Jerome O'Laughlin, “An Automated HPLC Method for the Fractionation of
Polychlorinated Biphenyls, Polychlorinated Dibenzo-p-dioxins, and
Polychlorinated Dibenzofurans in Fish Tissue on a Porous Graphitic
Carbon Column,” Environmental Toxicology and Chemistry 16:8 1590-1597,
1997.

5.	“Working with Carcinogens,” Department of Health, Education, &
Welfare, Public Health Service, Centers for Disease Control, NIOSH,
Publication 77-206, August 1977, NTIS PB-277256.

6.	“OSHA Safety and Health Standards, General Industry,” OSHA 2206,
29 CFR 1910.

7.	“Safety in Academic Chemistry Laboratories,” ACS Committee on
Chemical Safety, 1979.

8.	“Standard Methods for the Examination of Water and Wastewater,”
18th edition and later revisions, American Public Health Association,
1015 15th St, NW, Washington, DC 20005, 1-35: Section 1090 (Safety),
1992.

9.	“Method 613 – 2,3,7,8-Tetrachlorodibenzo-p-dioxin,” 40 CFR 136,
Appendix A, Section 4.1.

10.	Lamparski, L.L., and Nestrick, T.J., “Novel Extraction Device for
the Determination of Chlorinated Dibenzo-p-dioxins (PCDDs) and
Dibenzofurans (PCDFs) in Matrices Containing Water,” Chemosphere,
19:27-31, 1989.

11.	Provost, L.P., and Elder, R.S., “Interpretation of Percent
Recovery Data,” American Laboratory, 15: 56-83, 1983.

12.	“Standard Practice for Sampling Water,” ASTM Annual Book of
Standards, ASTM, 1916 Race Street, Philadelphia, PA 19103-1187, 1980.

13.	“Methods 330.4 and 330.5 for Total Residual Chlorine,” USEPA,
EMSL, Cincinnati, OH 45268, EPA 600/4-70-020, April 1979.

14.	“Handbook of Analytical Quality Control in Water and Wastewater
Laboratories,” USEPA EMSL, Cincinnati, OH 45268, EPA-600/4-79-019,
April 1979.

15.	“Analytical Procedures and Quality Assurance Plan for the
Determination of PCDD/PCDF in Fish”, U.S. Environmental Protection
Agency, Environmental Research Laboratory, Duluth, MN 55804,
EPA/600/3-90/022, April 1990.

16.	Storr-Hansen, E. and T. Cederberg, “Determination of Coplanar
Polychlorinated Biphenyl (CB) Congeners in Seal Tissues by
Chromatography on Active Carbon, Dual-Column High Resolution GC/ECD and
High Resolution GC/High Resolution MS,” Chemosphere 24:9, 1181-1196,
1992.

17.	Echols, Kathy R., Robert W. Gale, Kevin Feltz, Jerome O'Laughlin,
Donald E. Tillitt, and Ted R. Schwartz, “Loading capacity and
chromatographic behavior of a porous graphitic carbon column for
polychlorinated biphenyls,” J. Chromatog. A 811: 135-144, 1998.

18.	Tessari, J.D., Personal communication with Dale Rushneck, available
from U.S. Environmental Protection Agency, Engineering and Analysis
Division (4303T), 1200 Pennsylvania Avenue NW, Washington, DC 20460.

19.	Ferrario, J.C., C. Byrne, A.E. Dupuy, Jr., “Background
Contamination by Coplanar Polychlorinated Biphenyls (PCBs) in Trace
Level High Resolution Gas Chromatography/High Resolution Mass
Spectrometry (HRGC/HRMS) Analytical Procedures” Chemosphere 34:11,
2451-2465, 1997.

20.	“Development of a Full Congener Version of Method 1668 and
Application to the Analysis of 209 PCB Congeners in Aroclors,” AXYS
Analytical Services, available from U.S. Environmental Protection
Agency, Engineering and Analysis Division (4303T), 1200 Pennsylvania
Avenue NW, Washington, DC 20460.

21.	“Method 1668A Interlaboratory Validation Study Report,” March
2010, EPA-820-R-10-004, U.S. Environmental Protection Agency,
Engineering and Analysis Division (4303T), 1200 Pennsylvania Avenue NW,
Washington, DC 20460.

22.	“Method 1668A Interlaboratory Study Report Addendum,” March
2010, EPA-820-R-10-003, U.S. Environmental Protection Agency,
Engineering and Analysis Division (4303T), 1200 Pennsylvania Avenue NW,
Washington, DC 20460.

23.	“Development of Pooled Method Detection Limits (MDLs) and Minimum
Levels of Quantitation (MLs) for EPA Method 1668C,” Brian Englert,
USEPA, May 18 2010; available from U.S. Environmental Protection Agency,
Engineering and Analysis Division (4303T), 1200 Pennsylvania Avenue NW,
Washington, DC 20460.

23.0	Tables and Figures

Table 1.	Names, Congener Numbers, and CAS Registry Numbers for Native
and Labeled Chlorinated Biphenyl (CB) Congeners Determined by Isotope
Dilution and Internal Standard HRGC/HRMS

CB congener name1	Congener number	CAS Registry number	Labeled analog
name	Labeled analog congener number	CAS Registry number

2-MoCB	1	2051-60-7	13C12-2-MoCB2	1L	234432-85-0

3-MoCB	2	2051-61-8

	4-MoCB	3	2051-62-9	13C12-4-MoCB2	3L	208263-77-8

2,2'-DiCB	4	13029-08-8	13C12-2,2'-DiCB2	4L	234432-86-1

2,3-DiCB	5	16605-91-7

	2,3'-DiCB	6	25569-80-6

	2,4-DiCB	7	33284-50-3

	2,4'-DiCB3	8	34883-43-7

	2,5-DiCB	9	34883-39-1	13C12-2,5-DiCB4	9L	250694-89-4

2,6-DiCB	10	33146-45-1

	3,3'-DiCB	11	2050-67-1

	3,4-DiCB	12	2974-92-7

	3,4'-DiCB	13	2974-90-5

	3,5-DiCB	14	34883-41-5

	4,4'-DiCB	15	2050-68-2	13C12-4,4'-DiCB2	15L	208263-67-6

2,2',3-TrCB	16	38444-78-9

	2,2',4-TrCB	17	37680-66-3

	2,2',5-TrCB3	18	37680-65-2

	2,2',6-TrCB	19	38444-73-4	13C12-2,2',6-TrCB2	19L	234432-87-2

2,3,3'-TrCB	20	38444-84-7

	 2,3,4-TrCB	21	55702-46-0

	2,3,4'-TrCB	22	38444-85-8

	2,3,5-TrCB	23	55720-44-0

	2,3,6-TrCB	24	55702-45-9

	2,3',4-TrCB	25	55712-37-3

	2,3',5-TrCB	26	38444-81-4

	 2,3',6-TrCB	27	38444-76-7

	 2,4,4'-TrCB3	28	7012-37-5	13C12-2,4,4'-TriCB5	28L	208263-76-7

2,4,5-TrCB	29	15862-07-4

	 2,4,6-TrCB	30	35693-92-6

	2,4',5-TrCB	31	16606-02-3

	 2,4',6-TrCB	32	38444-77-8

	2',3,4-TrCB	33	38444-86-9

	2',3,5-TrCB	34	37680-68-5

	3,3',4-TrCB	35	37680-69-6

	3,3',5-TrCB	36	38444-87-0

	3,4,4'-TrCB	37	38444-90-5	13C12-3,4,4'-TrCB2	37L	208263-79-0

3,4,5-TrCB	38	53555-66-1

	3,4',5-TrCB	39	38444-88-1

	2,2',3,3'-TeCB	40	38444-93-8

	 2,2',3,4-TeCB	41	52663-59-9

	2,2',3,4'-TeCB	42	36559-22-5

	 2,2',3,5-TeCB	43	70362-46-8

	2,2',3,5'-TeCB3	44	41464-39-5

	2,2',3,6-TeCB	45	70362-45-7

	2,2',3,6'-TeCB	46	41464-47-5

	2,2',4,4'-TeCB	47	2437-79-8

	2,2',4,5-TeCB	48	70362-47-9

	2,2',4,5'-TeCB	49	41464-40-8

	2,2',4,6-TeCB	50	62796-65-0

	2,2',4,6'-TeCB	51	68194-04-7

	2,2',5,5'-TeCB3	52	35693-99-3	13C12-2,2',5,5'-TeCB4	52L	208263-80-3

2,2',5,6'-TeCB	53	41464-41-9

	2,2',6,6'-TeCB	54	15968-05-5	13C12-2,2',6,6'-TeCB2	54L	234432-88-3

2,3,3',4'-TeCB	55	74338-24-2

	2,3,3',4'-TeCB	56	41464-43-1

	2,3,3',5-TeCB	57	70424-67-8

	2,3,3',5'-TeCB	58	41464-49-7

	2,3,3',6-TeCB	59	74472-33-6

	 2,3,4,4'-TeCB	60	33025-41-1

	 2,3,4,5-TeCB	61	33284-53-6

	 2,3,4,6-TeCB	62	54230-22-7

	 2,3,4',5-TeCB	63	74472-34-7

	2,3,4',6-TeCB	64	52663-58-8

	 2,3,5,6-TeCB	65	33284-54-7

	2,3',4,4'-TeCB3	66	32598-10-0

	 2,3',4,5-TeCB	67	73575-53-8

	 2,3',4,5'-TeCB	68	73575-52-7

	 2,3',4,6-TeCB	69	60233-24-1

	2,3',4',5-TeCB	70	32598-11-1

	 2,3',4',6-TeCB	71	41464-46-4

	 2,3',5,5'-TeCB	72	41464-42-0

	 2,3',5',6-TeCB	73	74338-23-1

	2,4,4',5-TeCB	74	32690-93-0

	2,4,4',6-TeCB	75	32598-12-2

	 2',3,4,5-TeCB	76	70362-48-0

	3,3',4,4'-TeCB3,6	77	32598-13-3	13C12-3,3',4,4'-TeCB2,7	77L	105600-23-5

3,3',4,5-TeCB	78	70362-49-1

	3,3',4,5'-TeCB	79	41464-48-6

	3,3',5,5'-TeCB	80	33284-52-5

	3,4,4',5-TeCB6	81	70362-50-4	13C12-3,4,4',5-TeCB7	81L	208461-24-9

2,2',3,3',4-PeCB	82	52663-62-4

	2,2',3,3',5-PeCB	83	60145-20-2

	2,2',3,3',6-PeCB	84	52663-60-2

	2,2',3,4,4'-PeCB	85	65510-45-4

	2,2',3,4,5-PeCB	86	55312-69-1

	2,2',3,4,5'-PeCB	87	38380-02-8

	2,2',3,4,6-PeCB	88	55215-17-3

	  2,2',3,4,6'-PeCB	89	73575-57-2

	2,2',3,4',5-PeCB	90	68194-07-0

	2,2',3,4',6-PeCB	91	68194-05-8

	 2,2',3,5,5'-PeCB	92	52663-61-3

	 2,2',3,5,6-PeCB	93	73575-56-1

	 2,2',3,5,6'-PeCB	94	73575-55-0

	2,2',3,5',6-PeCB	95	38379-99-6

	2,2',3,6,6'-PeCB	96	73575-54-9

	2,2',3',4,5-PeCB	97	41464-51-1

	 2,2',3',4,6-PeCB	98	60233-25-2

	2,2',4,4',5-PeCB	99	38380-01-7

	2,2',4,4',6-PeCB	100	39485-83-1

	2,2',4,5,5'-PeCB3	101	37680-73-2	13C12-2,2',4,5,5'-PeCB4	101L
104130-39-4

2,2',4,5,6'-PeCB	102	68194-06-9

	2,2',4,5,'6-PeCB	103	60145-21-3

	2,2',4,6,6'-PeCB	104	56558-16-8	13C12-2,2',4,6,6'-PeCB2	104L
234432-89-4

2,3,3',4,4'-PeCB3,6	105	32598-14-4	13C12-2,3,3',4,4'-PeCB7	105L
208263-62-1

 2,3,3',4,5-PeCB	106	70424-69-0

	2,3,3',4',5-PeCB	107	70424-68-9

	2,3,3',4,5'-PeCB	108	70362-41-3

	2,3,3',4,6-PeCB	109	74472-35-8

	2,3,3',4',6-PeCB	110	38380-03-9

	 2,3,3',5,5'-PeCB	111	39635-32-0	13C12-2,3,3',5,5'-PeCB5	111 L
235416-29-2

 2,3,3',5,6-PeCB	112	74472-36-9

	2,3,3',5',6-PeCB	113	68194-10-5

	2,3,4,4',5-PeCB6	114	74472-37-0	13C12-2,3,4,4',5-PeCB7	114 L
208263-63-2

 2,3,4,4',6-PeCB	115	74472-38-1

	 2,3,4,5,6-PeCB	116	18259-05-7

	2,3,4',5,6-PeCB	117	68194-11-6

	2,3',4,4',5-PeCB3,6	118	31508-00-6	13C12-2,3',4,4',5-PeCB7	118 L
104130-40-7

2,3',4,4',6-PeCB	119	56558-17-9

	2,3',4,5,5'-PeCB	120	68194-12-7

	2,3',4,5,'6-PeCB	121	56558-18-0

	2',3,3',4,5-PeCB	122	76842-07-4

	2',3,4,4',5-PeCB6	123	65510-44-3	13C12-2',3,4,4',5-PeCB7	123L
208263-64-3

 2',3,4,5,5'-PeCB	124	70424-70-3

	 2',3,4,5,6'-PeCB	125	74472-39-2

	3,3',4,4',5-PeCB3,6	126	57465-28-8	13C12-3,3',4,4',5-PeCB2,7	126L
208263-65-4

3,3',4,5,5'-PeCB	127	39635-33-1

	2,2',3,3',4,4'-HxCB3	128	38380-07-3

	2,2',3,3',4,5-HxCB	129	55215-18-4

	2,2',3,3',4,5'-HxCB	130	52663-66-8

	2,2',3,3',4,6-HxCB	131	61798-70-7

	2,2',3,3',4,6'-HxCB	132	38380-05-1

	2,2',3,3',5,5'-HxCB	133	35694-04-3

	2,2',3,3',5,6-HxCB	134	52704-70-8

	2,2',3,3',5,6'-HxCB	135	52744-13-5

	2,2',3,3',6,6'-HxCB	136	38411-22-2

	2,2',3,4,4',5-HxCB	137	35694-06-5

	2,2',3,4,4',5'-HxCB3	138	35065-28-2	13C12-2,2',3,4,4',5'-HxCB4	138L
208263-66-5

 2,2',3,4,4',6-HxCB	139	56030-56-9

	 2,2',3,4,4',6'-HxCB	140	59291-64-4

	2,2',3,4,5,5'-HxCB	141	52712-04-6

	2,2',3,4,5,6-HxCB	142	41411-61-4

	 2,2',3,4,5,6'-HxCB	143	68194-15-0

	2,2',3,4,5',6-HxCB	144	68194-14-9

	2,2',3,4,6,6'-HxCB	145	74472-40-5

	2,2',3,4',5,5'-HxCB	146	51908-16-8

	2,2',3,4',5,6-HxCB	147	68194-13-8

	2,2',3,4',5,6'-HxCB	148	74472-41-6

	2,2',3,4',5',6-HxCB	149	38380-04-0

	2,2',3,4',6,6'-HxCB	150	68194-08-1

	2,2',3,5,5',6-HxCB	151	52663-63-5

	2,2',3,5,6,6'-HxCB	152	68194-09-2

	2,2',4,4',5,5'-HxCB3	153	35065-27-1

	2,2',4,4',5',6-HxCB	154	60145-22-4

	2,2',4,4',6,6'-HxCB	155	33979-03-2	13C12-2,2',4,4',6,6'-HxCB2	155L
234432-90-7

2,3,3',4,4',5-HxCB6	156	38380-08-4	13C12-2,3,3',4,4',5-HxCB7	156L
208263-68-7

2,3,3',4,4',5'-HxCB6	157	69782-90-7	13C12-2,3,3',4,4',5'-HxCB7	157L
235416-30-5

2,3,3',4,4',6-HxCB	158	74472-42-7

	2,3,3',4,5,5'-HxCB	159	39635-35-3

	 2,3,3',4,5,6-HxCB	160	41411-62-5

	2,3,3',4,5',6-HxCB	161	74472-43-8

	2,3,3',4',5,5'-HxCB	162	39635-34-2

	 2,3,3',4',5,6-HxCB	163	74472-44-9

	 2,3,3',4',5',6-HxCB	164	74472-45-0

	 2,3,3',5,5',6-HxCB	165	74472-46-1

	 2,3,4,4',5,6-HxCB	166	41411-63-6

	2,3',4,4',5,5'-HxCB6	167	52663-72-6	13C12-2,3',4,4',5,5'-HxCB7	167L
208263-69-8

2,3',4,4',5',6-HxCB	168	59291-65-5

	3,3',4,4',5,5'-HxCB3,6	169	32774-16-6	13C12-3,3',4,4',5,5'-HxCB2,7	169L
208263-70-1

2,2',3,3',4,4',5-HpCB3	170	35065-30-6	13C12-2,2',3,3',4,4',5-HpCB	170L
160901-80-4

2,2'3,3',4,4',6-HpCB	171	52663-71-5

	2,2',3,3',4,5,5'-HpCB	172	52663-74-8

	2,2',3,3',4,5,6-HpCB	173	68194-16-1

	2,2',3,3',4,5,6'-HpCB	174	38411-25-5

	2,2',3,3',4,5',6-HpCB	175	40186-70-7

	2,2',3,3',4,6,6'-HpCB	176	52663-65-7

	2,2',3,3',4',5,6-HpCB	177	52663-70-4

	2,2',3,3',5,5',6-HpCB	178	52663-67-9	13C12-2,2',3,3',5,5',6-HpCB5	178L
232919-67-4

2,2',3,3',5,6,6'-HpCB	179	52663-64-6

	2,2',3,4,4',5,5'-HpCB3	180	35065-29-3	13C12-2,2',3,4,4',5,5'-HpCB	180L
160901-82-6

 2,2',3,4,4',5,6-HpCB	181	74472-47-2

	 2,2',3,4,4',5,6'-HpCB	182	60145-23-5

	2,2',3,4,4',5',6-HpCB	183	52663-69-1

	2,2',3,4,4',6,6'-HpCB	184	74472-48-3

	2,2',3,4,5,5',6-HpCB	185	52712-05-7

	2,2',3,4,5,6,6'-HpCB	186	74472-49-4

	2,2',3,4',5,5',6-HpCB3	187	52663-68-0

	2,2',3,4',5,6,6'-HpCB	188	74487-85-7	13C12-2,2',3,4',5,6,6'-HpCB2	188L
234432-91-8

2,3,3',4,4',5,5'-HpCB6	189	39635-31-9	13C12-2,3,3',4,4',5,5'-HpCB2,7
189L	208263-73-4

2,3,3',4,4',5,6-HpCB	190	41411-64-7

	2,3,3',4,4',5',6-HpCB	191	74472-50-7

	2,3,3',4,5,5',6-HpCB	192	74472-51-8

	2,3,3',4',5,5',6-HpCB	193	69782-91-8

	2,2',3,3',4,4',5,5'-OcCB	194	35694-08-7	13C12-2,2',3,3',4,4',5,5'-OcCB4
194L	208263-74-5

2,2',3,3',4,4',5,6-OcCB3	195	52663-78-2

	2,2',3,3',4,4',5,6'-OcCB	196	42740-50-1

	2,2',3,3',4,4',6,6'-OcCB	197	33091-17-7

	2,2',3,3',4,5,5',6-OcCB	198	68194-17-2

	2,2',3,3',4,5,5',6'-OcCB	199	52663-75-9

	2,2',3,3',4,5,6,6'-OcCB	200	52663-73-7

	2,2',3,3',4,5',6,6'-OcCB	201	40186-71-8

	2,2',3,3',5,5',6,6'-OcCB	202	2136-99-4	13C12-2,2',3,3',5,5',6,6'-OcCB2
202L	105600-26-8

2,2',3,4,4',5,5',6-OcCB	203	52663-76-0

	2,2',3,4,4',5,6,6'-OcCB	204	74472-52-9

	2,3,3',4,4',5,5',6-OcCB	205	74472-53-0	13C12-2,3,3',4,4',5,5',6-OcCB2
205L	234446-64-1

2,2',3,3',4,4',5,5',6-NoCB3	206	40186-72-9
13C12-2,2',3,3',4,4',5,5',6-NoCB2	206L	208263-75-6

2,2',3,3',4,4',5,6,6'-NoCB	207	52663-79-3

	2,2',3,3',4,5,5',6,6'-NoCB	208	52663-77-1
13C12-2,2',3,3',4,5,5',6,6'-NoCB2	208L	234432-92-9

DeCB3	209	2051-24-3	13C12-DeCB2	209L	105600-27-9

.	Abbreviations for chlorination levels

MoCB	monochlorobiphenyl	HxCB	hexachlorobiphenyl

DiCB	dichlorobiphenyl	HpCB	heptachlorobiphenyl

TrCB	trichlorobiphenyl	OcCB	octachlorobiphenyl

TeCB	tetrachlorobiphenyl	NoCB	nonachlorobiphenyl

PeCB	pentachlorobiphenyl	DeCB	decachlorobiphenyl

2.	Labeled level of chlorination (LOC) window-defining congener

3.	National Oceanic and Atmospheric Administration (NOAA) congener of
interest

4.	Labeled injection internal standard

5.	Labeled clean-up standard

6.	World Health Organization (WHO) toxic congener

7.	Labeled analog of WHO toxic congener

Table 2.   Retention times (RT), RT references, relative retention times
(RRTs), method detection limits (MDLs), and minimum levels of
quantitation (MLs) for the 209 CB congeners on SPB-octyl.

Cl

No. 1

Congener No. 2,3

RT Ref4

RT5

RRT6

RRT limits7

Window

(sec) 8

Quantitation reference9	Detection limits and minimum levels -

Matrix and concentration10

Water

 (pg/L)	Other

 (ng/kg)	Extract

(pg/µL)

MDL	ML	MDL	ML	ML

Compounds using 9L (13C12-2,5-DiCB) as Labeled injection internal
standard

	CB congener

		Monochlorobiphenyls

1	1	1L	13:44	1.0012	0.9988-1.0036	-1+3	1L	10	20	1.0	2	1

1	2	3L	16:08	0.9878	0.9847-0.9908	6	1L/3L	7	20	0.7	2	1

1	3	3L	16:21	1.0010	0.9990-1.0031	-1+3	3L	11	50	1.1	5	2.5

		Dichlorobiphenyls

2	4	4L	16:40	1.0010	0.9990-1.0030	-1+3	4L	13	50	1.3	5	2.5

2	10	4L	16:53	1.0140	1.0110-1.0170	6	4L/15L	13	50	1.3	5	2.5

2	9	4L	18:55	1.1361	1.1331-1.1391	6	4L/15L	7	20	0.7	2	1

2	7	4L	19:07	1.1481	1.1451-1.1512	6	4L/15L	8	20	0.8	2	1

2	6	4L	19:26	1.1672	1.1642-1.1702	6	4L/15L	7	20	0.7	2	1

2	5	4L	19:48	1.1892	1.1862-1.1922	6	4L/15L	8	20	0.8	2	1

2	8	4L	19:56	1.1972	1.1942-1.2002	6	4L/15L	15	50	1.5	5	2.5

2	14	15L	21:42	0.9267	0.9246-0.9288	6	4L/15L	8	20	0.8	2	1

2	11	15L	22:42	0.9694	0.9673-0.9715	6	4L/15L	34	100	3.4	10	5

2	13	15L	23:03	0.9843	0.9822-0.9865	6	4L/15L	19	50	1.9	5	2.5

2	12	15L	23:06	0.9865	0.9843-0.9886	6	4L/15L

	2	13/12	15L	23:04	0.9851	0.9829-0.9872	6	4L/15L

	2	15	15L	23:26	1.0007	0.9993-1.0021	-1+3	15L	16	50	1.6	5	2.5

		Trichlorobiphenyls

3	19	19L	20:19	1.0008	0.9992-1.0025	-1+3	19L	8	20	0.8	2	1

3	30	19L	22:15	1.0961	1.0936-1.0985	6	19L/37L	16	50	1.6	5	2.5

3	18	19L	22:23	1.1026	1.1002-1.1051	6	19L/37L

	3	30/18	19L	22:19	1.0993	1.0969-1.1018	6	19L/37L

	3	17	19L	22:49	1.1240	1.1215-1.1264	6	19L/37L	9	20	0.9	2	1

3	27	19L	23:06	1.1379	1.1355-1.1404	6	19L/37L	8	20	0.8	2	1

3	24	19L	23:14	1.1445	1.1420-1.1470	6	19L/37L	10	20	1.0	2	1

3	16	19L	23:25	1.1535	1.1511-1.1560	6	19L/37L	9	20	0.9	2	1

3	32	19L	24:57	1.2291	1.2266-1.2315	6	19L/37L	8	20	0.8	2	1

3	34	19L	25:17	1.2455	1.2430-1.2479	6	19L/37L	7	20	0.7	2	1

3	23	19L	25:26	1.2529	1.2504-1.2553	6	19L/37L	7	20	0.7	2	1

3	29	19L	25:47	1.2701	1.2660-1.2742	10	19L/37L	12	50	1.2	5	2.5

3	26	19L	25:48	1.2709	1.2668-1.2750	10	19L/37L

	3	29/26	19L	25:48	1.2709	1.2668-1.2750	10	19L/37L

	3	25	37L	26:04	0.8364	0.8348-0.8380	6	19L/37L	8	20	0.8	2	1

3	31	37L	26:25	0.8476	0.8460-0.8492	6	19L/37L	18	50	1.8	5	2.5

3	28	37L	26:44	0.8578	0.8551-0.8604	10	19L/37L	22	50	2.2	5	2.5

3	20	37L	26:49	0.8604	0.8578-0.8631	10	19L/37L

	3	28/20	37L	26:47	0.8594	0.8567-0.8620	10	19L/37L

	3	21	37L	26:58	0.8652	0.8626-0.8679	10	19L/37L	21	50	2.1	5	2.5

3	33	37L	27:01	0.8668	0.8642-0.8695	10	19L/37L

	3	21/33	37L	26:59	0.8658	0.8631-0.8684	10	19L/37L

	3	22	37L	27:29	0.8818	0.8802-0.8834	6	19L/37L	9	20	0.9	2	1

3	36	37L	29:05	0.9332	0.9316-0.9348	6	19L/37L	8	20	0.8	2	1

3	39	37L	29:30	0.9465	0.9449-0.9481	6	19L/37L	8	20	0.8	2	1

3	38	37L	30:10	0.9679	0.9663-0.9695	6	19L/37L	7	20	0.7	2	1

3	35	37L	30:42	0.9850	0.9834-0.9866	6	19L/37L	9	20	0.9	2	1

3	37	37L	31:11	1.0005	0.9995-1.0011	-1+3	37L	10	20	1.0	2	1

	Labeled Compounds

1	1L	9L	13:43	0.7257	0.7125-0.7390	30	9L

	1	3L	9L	16:20	0.8642	0.8510-0.8774	30	9L

	2	4L	9L	16:39	0.8810	0.8677-0.8942	30	9L

	2	15L	9L	23:25	1.2390	1.2302-1.2478	20	9L

	3	19L	9L	20:18	1.0741	1.0608-1.0873	30	9L

	3	37L	52L	31:10	1.0841	1.0754-1.0928	30	52L

	Compounds using 52L (13C12-2,2',5,5'-TeCB) as Labeled injection
internal standard

	CB congener

		Tetrachlorobiphenyls

4	54	54L	23:51	1.0007	0.9993-1.0021	-1+3	54L	14	50	1.4	5	2.5

4	50	54L	26:07	1.0958	1.0923-1.0993	10	54L/81L/77L	25	100	2.5	10	5

4	53	54L	26:09	1.0972	1.0937-1.1007	10	54L/81L/77L

	4	50/53	54L	26:08	1.0965	1.0930-1.1000	10	54L/81L/77L

	4	45	54L	26:55	1.1294	1.1259-1.1329	10	54L/81L/77L	22	50	2.2	5	2.5

4	51	54L	26:58	1.1315	1.1280-1.1350	10	54L/81L/77L

	4	45/51	54L	26:57	1.1308	1.1273-1.1343	10	54L/81L/77L

	4	46	54L	27:18	1.1455	1.1434-1.1476	6	54L/81L/77L	10	20	1.0	2	1

4	52	54L	28:45	1.2063	1.2042-1.2084	6	54L/81L/77L	15	50	1.5	5	2.5

4	73	54L	28:52	1.2112	1.2091-1.2133	6	54L/81L/77L	14	50	1.4	5	2.5

4	43	54L	28:58	1.2154	1.2133-1.2175	6	54L/81L/77L	14	50	1.4	5	2.5

4	69	54L	29:08	1.2224	1.2189-1.2259	10	54L/81L/77L	26	100	2.6	10	5

4	49	54L	29:16	1.2280	1.2245-1.2315	10	54L/81L/77L

	4	69/49	54L	29:12	1.2252	1.2217-1.2287	10	54L/81L/77L

	4	48	54L	29:33	1.2399	1.2378-1.2420	6	54L/81L/77L	14	50	1.4	5	2.5

4	65	54L	29:49	1.2510	1.2476-1.2545	10	54L/81L/77L	40	100	4.0	10	5

4	47	54L	29:50	1.2517	1.2483-1.2552	10	54L/81L/77L

	4	44	54L	29:53	1.2538	1.2503-1.2573	10	54L/81L/77L

	4	65/47/44	54L	29:50	1.2517	1.2483-1.2552	10	54L/81L/77L

	4	62	54L	30:06	1.2629	1.2594-1.2664	10	54L/81L/77L	37	100	3.7	10	5

4	75	54L	30:08	1.2643	1.2608-1.2678	10	54L/81L/77L

	4	59	54L	30:12	1.2671	1.2636-1.2706	10	54L/81L/77L

	4	62/75/59	54L	30:09	1.2650	1.2615-1.2685	10	54L/81L/77L

	4	42	54L	30:26	1.2769	1.2748-1.2790	6	54L/81L/77L	16	50	1.6	5	2.5

4	41	54L	30:52	1.2951	1.2916-1.2986	10	54L/81L/77L	42	100	4.2	10	5

4	71	54L	30:58	1.2993	1.2958-1.3028	10	54L/81L/77L

	4	40	54L	31:01	1.3014	1.2979-1.3049	10	54L/81L/77L

	4	41/71/40	54L	30:58	1.2993	1.2958-1.3028	10	54L/81L/77L

	4	64	54L	31:12	1.3091	1.3070-1.3112	6	54L/81L/77L	13	50	1.3	5	2.5

4	72	81L	31:59	0.8336	0.8323-0.8349	6	54L/81L/77L	13	50	1.3	5	2.5

4	68	81L	32:18	0.8419	0.8406-0.8432	6	54L/81L/77L	14	50	1.4	5	2.5

4	57	81L	32:46	0.8540	0.8527-0.8553	6	54L/81L/77L	11	50	1.1	5	2.5

4	58	81L	33:05	0.8623	0.8610-0.8636	6	54L/81L/77L	14	50	1.4	5	2.5

4	67	81L	33:13	0.8658	0.8645-0.8671	6	54L/81L/77L	12	50	1.2	5	2.5

4	63	81L	33:30	0.8732	0.8719-0.8745	6	54L/81L/77L	12	50	1.2	5	2.5

4	61	81L	33:46	0.8801	0.8775-0.8827	12	54L/81L/77L	59	200	5.9	20	10

4	70	81L	33:53	0.8831	0.8805-0.8858	12	54L/81L/77L

	4	76	81L	33:55	0.8840	0.8814-0.8866	12	54L/81L/77L

	4	74	81L	33:57	0.8849	0.8827-0.8871	10	54L/81L/77L

	4	61/70/76/74	81L	33:55	0.8840	0.8814-0.8866	12	54L/81L/77L

	4	66	81L	34:15	0.8927	0.8914-0.8940	6	54L/81L/77L	17	50	1.7	5	2.5

4	55	81L	34:28	0.8983	0.8970-0.8997	6	54L/81L/77L	12	50	1.2	5	2.5

4	56	81L	35:03	0.9136	0.9123-0.9149	6	54L/81L/77L	15	50	1.5	5	2.5

4	60	81L	35:16	0.9192	0.9179-0.9205	6	54L/81L/77L	14	50	1.4	5	2.5

4	80	81L	35:32	0.9262	0.9248-0.9275	6	54L/81L/77L	11	50	1.1	5	2.5

4	79	81L	37:16	0.9713	0.9700-0.9726	6	54L/81L/77L	13	50	1.3	5	2.5

4	78	81L	37:52	0.9870	0.9857-0.9883	6	54L/81L/77L	16	50	1.6	5	2.5

4	81	81L	38:23	1.0004	0.9996-1.0013	-1+3	81L	18	50	1.8	5	2.5

4	77	77L	39:02	1.0004	0.9996-1.0013	-1+3	77L	14	50	1.4	5	2.5

	Labeled compounds

4	54L	52L	23:50	0.8290	0.8232-0.8348	20	52L

	4	81L	52L	38:22	1.3345	1.3287-1.3403	20	52L

	4	77L	52L	39:01	1.3571	1.3513-1.3629	20	52L

	Compounds using 101L (13C12-2,2',4,5,5'-PeCB) as Labeled injection
internal standard

	CB congener

		Pentachlorobiphenyls

5	104	104L	29:46	1.0000	0.9994-1.0017	-1+3	104L	14	50	1.4	5	2.5

5	96	104L	30:17	1.0174	1.0146-1.0202	10	104L/123L/114L/118L/105L	15	50
1.5	5	2.5

5	103	104L	32:11	1.0812	1.0795-1.0829	6	104L/123L/114L/118L/105L	11	50
1.1	5	2.5

5	94	104L	32:29	1.0913	1.0896-1.0929	6	104L/123L/114L/118L/105L	13	50
1.3	5	2.5

5	95	104L	33:00	1.1086	1.1058-1.1114	10	104L/123L/114L/118L/105L	77	200
7.7	20	10

5	100	104L	33:06	1.1120	1.1092-1.1148	10	104L/123L/114L/118L/105L

	5	93	104L	33:14	1.1165	1.1137-1.1193	10	104L/123L/114L/118L/105L

	5	102	104L	33:21	1.1204	1.1176-1.1232	10	104L/123L/114L/118L/105L

	5	98	104L	33:26	1.1232	1.1204-1.1260	10	104L/123L/114L/118L/105L

	5	95/100/93/102/98	104L	33:13	1.1159	1.1131-1.1187	15
104L/123L/114L/118L/105L

	5	88	104L	33:48	1.1355	1.1321-1.1389	12	104L/123L/114L/118L/105L	22	50
2.2	5	2.5

5	91	104L	33:55	1.1394	1.1366-1.1422	10	104L/123L/114L/118L/105L

	5	88/91	104L	33:52	1.1377	1.1344-1.1411	12	104L/123L/114L/118L/105L

	5	84	104L	34:14	1.1501	1.1484-1.1517	6	104L/123L/114L/118L/105L	11	20
1.1	2	1

5	89	104L	34:44	1.1669	1.1652-1.1685	6	104L/123L/114L/118L/105L	13	50
1.3	5	2.5

5	121	104L	34:57	1.1741	1.1725-1.1758	6	104L/123L/114L/118L/105L	12	50
1.2	5	2.5

5	92	123L	35:26	0.8639	0.8627-0.8651	6	104L/123L/114L/118L/105L	13	50
1.3	5	2.5

5	113	123L	36:01	0.8781	0.8761-0.8801	10	104L/123L/114L/118L/105L	47	200
4.7	20	10

5	90	123L	36:03	0.8789	0.8769-0.8809	10	104L/123L/114L/118L/105L

	5	101	123L	36:04	0.8793	0.8773-0.8813	10	104L/123L/114L/118L/105L

	5	113/90/101	123L	36:03	0.8789	0.8769-0.8809	10
104L/123L/114L/118L/105L

	5	83	123L	36:39	0.8935	0.8911-0.8960	12	104L/123L/114L/118L/105L	29	100
2.9	10	5

5	99	123L	36:41	0.8944	0.8923-0.8964	10	104L/123L/114L/118L/105L

	5	83/99	123L	36:40	0.8939	0.8915-0.8964	12	104L/123L/114L/118L/105L

	5	112	123L	36:51	0.8984	0.8972-0.8996	6	104L/123L/114L/118L/105L	14	50
1.4	5	2.5

5	119	123L	37:12	0.9069	0.9037-0.9102	16	104L/123L/114L/118L/105L	74	200
7.4	20	10

5	109	123L	37:12	0.9069	0.9037-0.9102	16	104L/123L/114L/118L/105L

	5	86	123L	37:17	0.9090	0.9057-0.9122	16	104L/123L/114L/118L/105L

	5	97	123L	37:17	0.9090	0.9057-0.9122	16	104L/123L/114L/118L/105L

	5	125	123L	37:21	0.9106	0.9074-0.9139	16	104L/123L/114L/118L/105L

	5	87	123L	37:25	0.9122	0.9102-0.9143	10	104L/123L/114L/118L/105L

	5	119/109/86/97/125/87	123L	37:19	0.9098	0.9065-0.9130	16
104L/123L/114L/118L/105L

	5	117	123L	37:57	0.9252	0.9228-0.9277	12	104L/123L/114L/118L/105L	38
100	3.8	10	5

5	116	123L	38:02	0.9273	0.9248-0.9297	12	104L/123L/114L/118L/105L

	5	85	123L	38:05	0.9285	0.9265-0.9305	10	104L/123L/114L/118L/105L

	5	117/116/85	123L	38:00	0.9265	0.9240-0.9289	12
104L/123L/114L/118L/105L

	5	110	123L	38:16	0.9330	0.9309-0.9350	10	104L/123L/114L/118L/105L	39
100	3.9	10	5

5	115	123L	38:18	0.9338	0.9317-0.9358	10	104L/123L/114L/118L/105L

	5	110/115	123L	38:17	0.9334	0.9313-0.9354	10	104L/123L/114L/118L/105L

	5	82	123L	38:40	0.9427	0.9415-0.9439	6	104L/123L/114L/118L/105L	15	50
1.5	5	2.5

5	111	123L	38:52	0.9476	0.9464-0.9488	6	104L/123L/114L/118L/105L	14	50
1.4	5	2.5

5	120	123L	39:21	0.9594	0.9581-0.9606	6	104L/123L/114L/118L/105L	13	50
1.3	5	2.5

5	108	123L	40:39	0.9911	0.9890-0.9931	10	104L/123L/114L/118L/105L	29	100
2.9	10	5

5	124	123L	40:40	0.9915	0.9894-0.9935	10	104L/123L/114L/118L/105L

	5	108/124	123L	40:39	0.9911	0.9890-0.9931	10	104L/123L/114L/118L/105L

	5	107	123L	40:54	0.9972	0.9959-0.9984	6	104L/123L/114L/118L/105L	17	50
1.7	5	2.5

5	123	123L	41:02	1.0004	0.9996-1.0012	-1+3	123L	17	50	1.7	5	2.5

5	106	123L	41:10	1.0037	1.0024-1.0049	6	104L/123L/114L/118L/105L	17	50
1.7	5	2.5

5	118	118L	41:22	1.0004	0.9996-1.0012	-1+3	118L	30	100	3.0	10	5

5	122	118L	41:49	1.0113	1.0101-1.0125	6	104L/123L/114L/118L/105L	12	50
1.2	5	2.5

5	114	114L	41:58	1.0004	0.9999-1.0012	-1+3	114L	15	50	1.5	5	2.5

5	105	105L	42:43	0.9996	0.9996-1.0012	-2+3	105L	17	50	1.7	5	2.5

5	127	105L	44:09	1.0332	1.0320-1.0343	6	104L/123L/114L/118L/105L	14	50
1.4	5	2.5

5	126	126L	45:58	1.0004	0.9996-1.0011	-1+3	126L	16	50	1.6	5	2.5

	Labeled compounds

5	104L	101L	29:46	0.8257	0.8211-0.8303	20	101L

	5	123L	101L	41:01	1.1378	1.1331-1.1424	20	101L

	5	118L	101L	41:21	1.1470	1.1424-1.1516	20	101L

	5	114L	101L	41:57	1.1637	1.1590-1.1683	20	101L

	5	105L	101L	42:44	1.1854	1.1808-1.1900	20	101L

	5	126L	101L	45:57	1.2746	1.2700-1.2792	20	101L

	Compounds using 138L (13C12-2,2',3,4,4',5'-HxCB) as Labeled injection
internal standard

	CB congener

		Hexachlorobiphenyls

6	155	155L	35:44	1.0000	0.9995-1.0014	-1+3	155L	14	50	1.4	5	2.5

6	152	155L	36:07	1.0107	1.0093-1.0121	6	155L/156L/157L/167L/169L	14	50
1.4	5	2.5

6	150	155L	36:15	1.0145	1.0131-1.0159	6	155L/156L/157L/167L/169L	15	50
1.5	5	2.5

6	136	155L	36:44	1.0280	1.0266-1.0294	6	155L/156L/157L/167L/169L	16	50
1.6	5	2.5

6	145	155L	37:00	1.0354	1.0340-1.0368	6	155L/156L/157L/167L/169L	16	50
1.6	5	2.5

6	148	155L	34:26	1.0756	1.0742-1.0770	6	155L/156L/157L/167L/169L	14	50
1.4	5	2.5

6	151	155L	39:10	1.0961	1.0938-1.0984	10	155L/156L/157L/167L/169L	46	100
4.6	10	5

6	135	155L	39:17	1.0993	1.0970-1.1017	10	155L/156L/157L/167L/169L

	6	154	155L	39:21	1.1012	1.0989-1.1035	10	155L/156L/157L/167L/169L

	6	151/135/154	155L	39:15	1.0984	1.0961-1.1007	10
155L/156L/157L/167L/169L

	6	144	155L	39:47	1.1133	1.1119-1.1147	6	155L/156L/157L/167L/169L	15	50
1.5	5	2.5

6	147	155L	40:09	1.1236	1.1213-1.1259	10	155L/156L/157L/167L/169L	35	100
3.5	10	5

6	149	155L	40:12	1.1250	1.1227-1.1273	10	155L/156L/157L/167L/169L

	6	147/149	155L	40:10	1.1241	1.1217-1.1264	10	155L/156L/157L/167L/169L

	6	134	155L	40:27	1.1320	1.1297-1.1343	10	155L/156L/157L/167L/169L	33
100	3.3	10	5

6	143	155L	40:30	1.1334	1.1311-1.1357	10	155L/156L/157L/167L/169L

	6	134/143	155L	40:29	1.1329	1.1306-1.1353	10	155L/156L/157L/167L/169L

	6	139	155L	40:47	1.1413	1.1390-1.1437	10	155L/156L/157L/167L/169L	29
100	2.9	10	5

6	140	155L	40:48	1.1418	1.1395-1.1441	10	155L/156L/157L/167L/169L

	6	139/140	155L	40:47	1.1413	1.1390-1.1437	10	155L/156L/157L/167L/169L

	6	131	155L	41:03	1.1488	1.1474-1.1502	6	155L/156L/157L/167L/169L	17	50
1.7	5	2.5

6	142	155L	41:13	1.1535	1.1521-1.1549	6	155L/156L/157L/167L/169L	17	50
1.7	5	2.5

6	132	155L	41:36	1.1642	1.1618-1.1665	10	155L/156L/157L/167L/169L	16	50
1.6	5	2.5

6	133	155L	41:57	1.1740	1.1726-1.1754	6	155L/156L/157L/167L/169L	12	50
1.2	5	2.5

6	165	167L	42:23	0.8864	0.8853-0.8874	6	155L/156L/157L/167L/169L	13	50
1.3	5	2.5

6	146	167L	42:38	0.8916	0.8906-0.8926	6	155L/156L/157L/167L/169L	14	50
1.4	5	2.5

6	161	167L	42:47	0.8947	0.8937-0.8958	6	155L/156L/157L/167L/169L	13	50
1.3	5	2.5

6	153	167L	43:17	0.9052	0.9035-0.9069	10	155L/156L/157L/167L/169L	30	100
3.0	10	5

6	168	167L	43:21	0.9066	0.9048-0.9083	10	155L/156L/157L/167L/169L

	6	153/168	167L	43:19	0.9059	0.9041-0.9076	10	155L/156L/157L/167L/169L

	6	141	167L	43:34	0.9111	0.9101-0.9122	6	155L/156L/157L/167L/169L	17	50
1.7	5	2.5

6	130	167L	44:01	0.9205	0.9195-0.9216	6	155L/156L/157L/167L/169L	13	50
1.3	5	2.5

6	137	167L	44:14	0.9251	0.9240-0.9261	6	155L/156L/157L/167L/169L	15	50
1.5	5	2.5

6	164	167L	44:22	0.9278	0.9268-0.9289	6	155L/156L/157L/167L/169L	15	50
1.5	5	2.5

6	138	167L	44:42	0.9348	0.9324-0.9373	14	155L/156L/157L/167L/169L	63	200
6.3	20	10

6	163	167L	44:42	0.9348	0.9324-0.9373	14	155L/156L/157L/167L/169L

	6	129	167L	44:47	0.9366	0.9341-0.9390	14	155L/156L/157L/167L/169L

	6	160	167L	44:53	0.9387	0.9369-0.9404	10	155L/156L/157L/167L/169L

	6	138/163/129/160	167L	44:47	0.9366	0.9341-0.9390	14
155L/156L/157L/167L/169L

	6	158	167L	45:05	0.9428	0.9418-0.9439	6	155L/156L/157L/167L/169L	16	50
1.6	5	2.5

6	166	167L	45:59	0.9617	0.9599-0.9634	10	155L/156L/157L/167L/169L	29	100
2.9	10	5

6	128	167L	46:09	0.9651	0.9634-0.9669	10	155L/156L/157L/167L/169L

	6	128/166	167L	46:04	0.9634	0.9617-0.9651	10	155L/156L/157L/167L/169L

	6	159	167L	46:59	0.9826	0.9815-0.9836	6	155L/156L/157L/167L/169L	14	50
1.4	5	2.5

6	162	167L	47:18	0.9892	0.9881-0.9902	6	155L/156L/157L/167L/169L	13	50
1.3	5	2.5

6	167	167L	47:49	1.0000	0.9997-1.0010	-1+3	167L	13	50	1.3	5	2.5

6	156	156L/157L	49:05	0.9993	0.9983-1.0003	6	156L/157L	23	100	2.3	10	5

6	157	156L/157L	49:09	1.0007	0.9990-1.0024	10	156L/157L

	6	156/157	156L/157L	49:07	1.0000	0.9990-1.1010	6	156L/157L

	6	169	169L	52:31	1.0003	0.9997-1.0010	-1+3	169L	15	50	1.5	5	2.5

	Labeled compounds

6	155L	138L	35:44	0.7997	0.7960-0.8034	20	138L

	6	167L	138L	47:49	1.0701	1.0664-1.0739	20	138L

	6	156L	138L	49:05	1.0985	1.0974-1.0996	20	138L

	6	157L	138L	49:08	1.0996	1.0959-1.1033	20	138L

	6	156L/157L	138L	49:07	1.0992	1.0981-1.1003	20	138L

	6	169L	138L	52:30	1.1749	1.1738-1.1761	20	138L

	Compounds using 194L(13C12-2,2',3,3',4,4',5,5'-OcCB) as Labeled
injection internal standard

	CB congener

		Heptachlorobiphenyls

7	188	188L	41:51	1.0000	0.9996-1.0012	-1+3	188L	15	50	1.5	5	2.5

7	179	188L	42:19	1.0112	1.0100-1.0123	6	188L/189L	14	50	1.4	5	2.5

7	184	188L	42:45	1.0215	1.0203-1.0227	6	188L/189L	14	50	1.4	5	2.5

7	176	188L	43:15	1.0335	1.0323-1.0346	6	188L/189L	12	50	1.2	5	2.5

7	186	188L	43:45	1.0454	1.0442-1.0466	6	188L/189L	15	50	1.5	5	2.5

7	178	188L	45:06	1.0777	1.0765-1.0789	6	188L/189L	14	50	1.4	5	2.5

7	175	188L	45:46	1.0936	1.0924-1.0948	6	188L/189L	14	50	1.4	5	2.5

7	187	188L	46:02	1.1000	1.0988-1.1012	6	188L/189L	17	50	1.7	5	2.5

7	182	188L	46:14	1.1047	1.1035-1.1059	6	188L/189L	13	50	1.3	5	2.5

7	183	188L	46:42	1.1159	1.1147-1.1171	6	188L/189L	28	100	2.8	10	5

7	185	188L	46:53	1.1203	1.1191-1.1215	6	188L/189L

	7	183/185	188L	46:47	1.1179	1.1167-1.1191	6	188L/189L

	7	174	188L	47:02	1.1239	1.1227-1.1251	6	188L/189L	15	50	1.5	5	2.5

7	177	188L	47:30	1.1350	1.1338-1.1362	6	188L/189L	11	50	1.1	5	2.5

7	181	188L	47:52	1.1438	1.1426-1.1450	6	188L/189L	13	50	1.3	5	2.5

7	171	188L	48:10	1.1509	1.1489-1.1529	10	188L/189L	30	100	3.0	10	5

7	173	188L	48:11	1.1513	1.1501-1.1525	6	188L/189L

	7	171/173	188L	48:10	1.1509	1.1489-1.1529	6	188L/189L

	7	172	189L	49:47	0.9035	0.9026-0.9044	6	188L/189L	13	50	1.3	5	2.5

7	192	189L	50:06	0.9093	0.9083-0.9102	6	188L/189L	13	50	1.3	5	2.5

7	193	189L	50:26	0.9153	0.9144-0.9162	6	188L/189L	30	100	3.0	10	5

7	180	189L11	50:27	0.9156	0.9147-0.9165	6	188L/189L11

	7	193/180	189L	50:26	0.9153	0.9144-0.9162	6	188L/189L

	7	191	189L	50:51	0.9229	0.9220-0.9238	6	188L/189L	13	50	1.3	5	2.5

7	170	189L11	51:54	0.9419	0.9410-0.9428	6	188L/189L11	12	50	1.2	5	2.5

7	190	189L	52:26	0.9516	0.9507-0.9525	6	188L/189L	14	50	1.4	5	2.5

7	189	189L	55:07	1.0003	0.9997-1.0009	-1+3	189L	13	50	1.3	5	2.5

		Octachlorobiphenyls

8	202	202L	47:32	1.0004	0.9996-1.0011	-1+3	202L	24	100	2.4	10	5

8	201	202L	48:31	1.0210	1.0193-1.0228	10	202L/205L	20	50	2.0	5	2.5

8	204	202L	49:11	1.0351	1.0340-1.0361	6	202L/205L	21	50	2.1	5	2.5

8	197	202L	49:27	1.0407	1.0396-1.0417	6	202L/205L	43	100	4.3	10	5

8	200	202L	49:40	1.0452	1.0442-1.0463	6	202L/205L

	8	197/200	202L	49:33	1.0428	1.0417-1.0438	6	202L/205L

	8	198	202L	52:30	1.1049	1.1031-1.1066	10	202L/205L	37	100	3.7	10	5

8	199	202L	52:32	1.1056	1.1045-1.1066	6	202L/205L

	8	198/199	202L	52:31	1.1052	1.1035-1.1070	10	202L/205L

	8	196	205L	53:13	0.9207	0.9198-0.9216	6	202L/205L	20	50	2.0	5	2.5

8	203	205L	53:26	0.9245	0.9236-0.9253	6	202L/205L	18	50	1.8	5	2.5

8	195	205L	54:55	0.9501	0.9493-0.9510	6	202L/205L	22	50	2.2	5	2.5

8	194	205L	57:19	0.9916	0.9908-0.9925	6	202L/205L	18	50	1.8	5	2.5

8	205	205L	57:49	1.0003	0.9997-1.0009	-1+3	205L	15	50	1.5	5	2.5

		Nonachlorobiphenyls

9	208	208L	54:33	1.0003	0.9997-1.0009	-1+3	208L	16	50	1.6	5	2.5

9	207	208L	55:32	1.0183	1.0174-1.0193	6	208L/206L	19	50	1.9	5	2.5

9	206	206L	59:37	1.0003	0.9997-1.0008	-1+3	206L	16	50	1.6	5	2.5

		Decachlorobiphenyl

10	209	209L	61:15	1.0003	0.9997-1.0008	-1+3	209L	16	50	1.6	5	2.5

	Labeled compounds

7	188L	194L	41:51	0.7304	0.7275-0.7333	20	194L

	7	180L	194L	50:27	0.8805	0.8775-0.8834	20	194L

	7	170L	194L	51:53	0.9055	0.9026-0.9084	20	194L

	7	189L	194L	55:06	0.9616	0.9587-0.9645	20	194L

	8	202L	194L	47:31	0.8293	0.8264-0.8322	20	194L

	8	205L	194L	57:48	1.0087	1.0044-1.0131	30	194L

	9	208L	194L	54:32	0.9517	0.9488-0.9546	20	194L

	9	206L	194L	59:36	1.0401	1.0358-1.0445	30	194L

	10	209L	194L	61:14	1.0686	1.0643-1.0730	30	194L

	Labeled clean-up standards

3	28L	52L	26:44	0.9266	0.9209-0.9324	20	52L

	5	111L	101L	38:51	1.0777	1.0730-1.0823	20	101L

	7	178L	138L	45:05	1.0090	1.0052-1.0127	20	138L

	Labeled injection internal standards

2	9L	138L	18:54	0.4230	0.4183-0.4276	25	138L

	4	52L	138L	28:45	0.6434	0.6388-0.6481	25	138L

	5	101L	138L	36:03	0.8068	0.8021-0.8115	25	138L

	6	138L	138L	44:41	1.0000	0.9996-1.0011	100	138L

	8	194L	138L	57:18	1.2824	1.2777-1.2870	25	138L

1.	Number of chlorines on congener.

2.	Suffix “L” indicates labeled compound.

3.	Multiple congeners in a box indicates congeners that co-elute or may
not be adequately resolved on a 30-m SPB-octyl column.

4.	Retention time (RT) reference used to locate target congener.

5.	Retention time of target congener.

6.	Relative retention time (RRT) between the RT for the congener and RT
for the reference.

7.	RRT limits based on RT window.  RTs, RRTs, and RRT limits may differ
slightly from those in Table 2.

8.	RT window width necessary to attempt to unambiguously identify the
congener in the presence of other congeners.

9.	Labeled congeners that form the quantitation reference.  Areas from
the exact m/z’s of the congeners listed in the quantitation reference
are summed, and divided by the number of congeners in the quantitation
reference.  For example, for congener 10, the areas at the exact m/z’s
for 4L and 15L are summed and the sum is divided by 2 (because there are
2 congeners in the quantitation reference).

10.	MDLs for water pooled from data from AXYS Analytical,
TestAmerica-Knoxville, and Battelle-Columbus  (see Reference 24).  MLs
for water per ML procedure at 68 FR 11790.  MDLs and MLs for “Other”
and “Extract” calculated from sample amount and extract volume.

11.	If congeners 170L and 180L are included in the calibration and
spiking solutions, these congeners should be used as RT and quantitation
references.Table 3.	Concentrations of Native and Labeled Chlorinated
Biphenyls in Stock Solutions Spiking Solutions

CB Congener	Solution Concentrations

	Stock (µg/mL)	Spiking (ng/mL)	Extract (ng/mL)

  Native toxics/LOC1

1	20	1.0	50

3	20	1.0	50

4	20	1.0	50

15	20	1.0	50

19	20	1.0	50

37	20	1.0	50

54	20	1.0	50

77	20	1.0	50

81	20	1.0	50

104	20	1.0	50

105	20	1.0	50

114	20	1.0	50

118	20	1.0	50

123	20	1.0	50

126	20	1.0	50

155	20	1.0	50

156	20	1.0	50

157	20	1.0	50

167	20	1.0	50

169	20	1.0	50

188	20	1.0	50

189	20	1.0	50

202	20	1.0	50

205	20	1.0	50

206	20	1.0	50

208	20	1.0	50

209	20	1.0	50

  Native congener mix stock solutions2

	MoCB thru TrCB	2.5

	TeCB thru HpCB	5.0

	OcCB thru DeCB	7.5

  Labeled toxics/LOC/window-defining3

1L	1.0	2.0	100

3L	1.0	2.0	100

4L	1.0	2.0	100

15L	1.0	2.0	100

19L	1.0	2.0	100

37L	1.0	2.0	100

54L	1.0	2.0	100

77L	1.0	2.0	100

81L	1.0	2.0	100

104L	1.0	2.0	100

105L	1.0	2.0	100

114L	1.0	2.0	100

118L	1.0	2.0	100

123L	1.0	2.0	100

126L	1.0	2.0	100

155L	1.0	2.0	100

156L	1.0	2.0	100

157L	1.0	2.0	100

167L	1.0	2.0	100

169L	1.0	2.0	100

188L	1.0	2.0	100

189L	1.0	2.0	100

202L	1.0	.2.0	100

205L	1.0	2.0	100

206L	1.0	2.0	100

208L	1.0	2.0	100

209L	1.0	2.0	100

  Labeled clean-up4

28L	1.0	2.0	100

111L	1.0	2.0	100

178L	1.0	2.0	100

  Labeled injection internal5

9L	5.0	1000	100

52L	5.0	1000	100

101L	5.0	1000	100

138L	5.0	1000	100

194L	5.0	1000	100

 Diluted combined 209 congener6	Solution Concentration (ng/mL)

Standard	Native	Labeled

  Native congeners

      MoCB thru TrCB	25

	      TeCB thru HpCB	50

	      OcCB thru DeCB	75

	  Labeled toxics/LOC/window-defining

100

  Labeled cleanup

100

  Labeled injection internal

100

1.	Stock solution:  Section 7.8.1; Spiking solution: Section 7.11

2.	Section <IP_ADDRESS>

3.	Stock solution:  Section 7.9.1; Spiking solution: Section 7.12

4.	Stock solution:  Section 7.9.2; Spiking solution: Section 7.13

5.	Stock solution:  Section 7.9.3; Spiking solution: Section 7.14

6.	Section <IP_ADDRESS>.2

Table 4.	Composition of Individual Native CB Congener Solutions1

Solution Identifier

A2	B2	C2	D2	E2

Accu-Standard part number

M-1668A-1	M-1668A-2	M-1668A-3	M-1668A-4	M-1668A-5

2	7	13	25	1

10	5	17	21	3

9	12	29	69	4

6	18	20	47	15

8	24	46	42	19

14	23	65	a64	16

11	28	59	70	37

30	22	40	102	54

27	39	67	97	43

32	53	76	115	44

34	51	80	123	74

26	73	93	134	56

31	48	84	131	77

33	62	101	163	104

36	71	112	180	98

38	68	86

125

35	58	116

110

50	61	107

126

45	55	154

155

52	60	147

138

49	94	140

169

75	100	146

188

41	91	141

189

72	121	164

202

57	90	158

205

63	99	182

208

66	109	174

206

79	117	173

209

78	111	193

81	108

	96	118

	103	114

	95	150

	88	145

	89	135

	92	149

	113	139

	83	132

	119	165

	87	168

	85	137

	82	160

	120	128

	124	162

	106	157

	122	184

	105	186

	127	187

	152	185

	136	181

	148	192

	151	197

	144	199/201

	143	203

	142

133

161

153

130

129

166

159

167

156

179

176

178

175

183

177

171

172

191

170

190

201/200

204

200/199

198

196

195

194

207

Total number of congeners

83	54	29	15	28

1.	Congeners present in each standard solution are listed in elution
order for each level of chlorination.  Congener number (Table 1) listed
first; BZ number listed second, where ambiguous.  See Table 3 for
concentrations of congeners in stock solutions and Table 5 for
concentrations in calibration standards.

Table 5.	Concentration of Congeners in Calibration and Calibration
Verification Standards

Congener Name	Congener

No.1	Solution Concentration (ng/mL)

CS-0.2 (Hi sens)2	CS-1	CS-2	CS-3 (VER)	CS-4	CS-5

Native toxics/LOC

2-MoCB	1	0.20	1.0	5.0	50	400	2000

4-MoCB	3	0.20	1.0	5.0	50	400	2000

2,2'-DiCB	4	0.20	1.0	5.0	50	400	2000

4,4'-DiCB	15	0.20	1.0	5.0	50	400	2000

2,2',6'-TrCB	19	0.20	1.0	5.0	50	400	2000

3,4,4'-TrCB	37	0.20	1.0	5.0	50	400	2000

2,2',6,6'-TeCB	54	0.20	1.0	5.0	50	400	2000

3,3',4,4'-TeCB	77	0.20	1.0	5.0	50	400	2000

3,4,4',5-TeCB	81	0.20	1.0	5.0	50	400	2000

2,2',4,6,6'-PeCB	104	0.20	1.0	5.0	50	400	2000

2,3,3',4,4'-PeCB	105	0.20	1.0	5.0	50	 400	2000

2,3,4,4',5-PeCB	114	0.20	1.0	5.0	50	 400	2000

2,3',4,4',5-PeCB	118	0.20	1.0	5.0	50	 400	2000

2',3,4,4',5-PeCB	123	0.20	1.0	5.0	50	 400	2000

3,3',4,4',5-PeCB	126	0.20	1.0	5.0	50	 400	2000

2,2',4,4',6,6'-HxCB	155	0.20	1.0	5.0	50	400	2000

2,3,3',4,4',5-HxCB	156	0.20	1.0	5.0	50	 400	2000

2,3,3',4,4',5'-HxCB	157	0.20	1.0	5.0	 50	 400	2000

2,3',4,4',5,5'-HxCB	167	0.20	1.0	5.0	50	400	2000

3,3',4,4',5,5'-HxCB	169	0.20	1.0	5.0	 50	 400	2000

2,2',3,4',5,6,6'-HpCB	188	0.20	1.0	5.0	50	400	2000

2,3,3',4,4',5,5'-HpCB	189	0.20	1.0	5.0	50	400	2000

2,2',3,3',5,5',6,6'-OcCB	202	0.20	1.0	5.0	50	400	2000

2,3,3',4,4',5,5',6-OcCB	205	0.20	1.0	5.0	50	400	2000

2,2',3,3',4,4',5,5',6-NoCB	206	0.20	1.0	5.0	50	400	2000

2,2',3,3',4',5,5',6,6'-NoCB	208	0.20	1.0	5.0	50	400	2000

DeCB	209	0.20	1.0	5.0	 50	 400	2000

Labeled toxics/LOC/window-defining

13C12-2-MoCB	1L	100	100	100	100	100	100

13C12-4-MoCB	3L	100	100	100	100	100	100

13C12-2,2'-DiCB	4L	100	100	100	100	100	100

13C12-4,4'-DiCB	15L	100	100	100	100	100	100

13C12-2,2',6'-TrCB	19L	100	100	100	100	100	100

13C12-3,4,4'-TrCB	37L	100	100	100	100	100	100

13C12-2,2',6,6'-TeCB	54L	100	100	100	100	100	100

13C12-3,3',4,4'-TeCB	77L	100	100	100	100	100	100

13C12-3,4,4',5-TeCB	81L	100	100	100	100	100	100

13C12-2,2',4,6,6'-PeCB	104L	100	100	100	100	100	100

13C12-2,3,3',4,4'-PeCB	105L	100	100	100	100	100	100

13C12-2,3,4,4',5-PeCB	114L	100	100	100	100	100	100

13C12-2,3',4,4',5-PeCB	118L	100	100	100	100	100	100

13C12-2',3,4,4',5-PeCB	123L	100	100	100	100	100	100

13C12-3,3',4,4',5-PeCB	126L	100	100	100	100	100	100

13C12-2,2',4,4',6,6'-HxCB	155L	100	100	100	100	100	100

13C12-2,3,3',4,4',5-HxCB	156L	100	100	100	100	100	100

13C12-2,3,3',4,4',5'-HxCB	157L	100	100	100	100	100	100

13C12-2,3',4,4',5,5'-HxCB	167L	100	100	100	100	100	100

13C12-3,3',4,4',5,5'-HxCB	169L	100	100	100	100	100	100

13C12-2,2',3,4',5,6,6'-HpCB	188L	100	100	100	100	100	100

13C12-2,3,3',4,4',5,5'-HpCB	189L	100	100	100	100	100	100

13C12-2,2',3,3',5,5',6,6'-OcCB	202L	100	100	100	100	100	100

13C12-2,3,3',4,4',5,5',6-OcCB	205L	100	100	100	100	100	100

13C12-2,2',3,3',4,4',5,5',6-NoCB	206L	100	100	100	100	100	100

13C12-2,2',3,3',4',5,5',6,6'-NoCB	208L	100	100	100	100	100	100

13C12-DeCB	209L	100	100	100	100	100	100

Labeled clean-up

13C12-2,4,4'-TrCB	28L	100	100	100	100	100	100

13C12-2,3,3',5,5'-PeCB	111L	100	100	100	100	 100	100

13C12-2,2',3,3',5,5',6-HpCB	178L	100	100	100	100	100	100

Labeled injection internal

13C12-2,5-DiCB	9L	100	100	100	100	100	100

13C12-2,2',5,5'-TeCB	52L	100	100	100	100	100	100

13C12-2,2',4',5,5'-PeCB	101L	100	100	100	100	100	100

13C12-2,2',3',4,4',5'-HxCB	138L	100	100	100	100	100	100

13C12-2,2',3,3',4,4',5,5'-OcCB	194L	100	100	100	100	100	100

1.	Suffix “L” indicates labeled compound.

2.	Additional concentration used for calibration of high sensitivity
HRGC/HRMS systems.  If the ion abundance ratio (Table 8) cannot be
achieved at this level (see Section 10.3.3), a calibration point at 0.4
or 0.5 ng/mL may be used.

Table 6.	QC Acceptance Criteria for VER, IPR, OPR, and Labeled
Compounds in Samples1,2

Congener Name	Congener No.3	Test Conc. (ng/mL)4

VER (%)5	IPR

OPR Recovery (%)	Labeled Compound Recovery in Samples (%)

RSD (%)	Mean Recovery (%)

2-MoCB	1	50	75 - 125	25	70 - 130	60 - 135	NA

4-MoCB	3	50	75 - 125	25	70 - 130	60 - 135

	2,2'-DiCB	4	50	75 - 125	25	70 - 130	60 - 135

	4,4'-DiCB	15	50	75 - 125	25	70 - 130	60 - 135

	2,2'6-TrCB	19	50	75 - 125	25	70 - 130	60 - 135

	3,4,4'-TrCB	37	50	75 - 125	25	70 - 130	60 - 135

	2,2'6,6'TeCB	54	50	75 - 125	25	70 - 130	60 - 135

	3,3',4,4'-TeCB	77	50	75 - 125	25	70 - 130	60 - 135

	3,4,4',5-TeCB	81	50	75 - 125	25	70 - 130	60 - 135

	2,2',4,6,6'-PeCB	104	50	75 - 125	25	70 - 130	60 - 135

	2,3,3',4,4'-PeCB	105	50	75 - 125	25	70 - 130	60 - 135

	2,3,4,4',5-PeCB	114	50	75 - 125	25	70 - 130	60 - 135

	2,3',4,4',5-PeCB	118	50	75 - 125	25	70 - 130	60 - 135

	2',3,4,4',5-PeCB	123	50	75 - 125	25	70 - 130	60 - 135

	3,3',4,4',5-PeCB	126	50	75 - 125	25	70 - 130	60 - 135

	2,2',4,4',6,6'-HxCB	155	50	75 - 125	25	70 - 130	60 - 135

	2,3,3',4,4',5-HxCB 6	156	50	75 - 125	25	70 - 130	60 - 135

	 2,3,3',4,4',5'-HxCB 6	157	50	75 - 125	25	70 - 130	60 - 135

	2,3',4,4',5,5'-HxCB	167	50	75 - 125	25	70 - 130	60 - 135

	3,3',4,4',5,5'-HxCB	169	50	75 - 125	25	70 - 130	60 - 135

	2,2',3,4',5,6,6'-HpCB	188	50	75 - 125	25	70 - 130	60 - 135

	2,3,3',4,4',5,5'-HpCB	189	50	75 - 125	25	70 - 130	60 - 135

	2,2',3,3',5,5',6,6'-OcCB	202	50	75 - 125	25	70 - 130	60 - 135

	2,3,3',4,4',5,5',6-OcCB	205	50	75 - 125	25	70 - 130	60 - 135

	2,2',3,3',4,4',5,5',6-NoCB	206	50	75 - 125	25	70 - 130	60 - 135

	2,2',3,3,'4,5,5',6,6'-NoCB	208	50	75 - 125	25	70 - 130	60 - 135

	DeCB	209	50	75 - 125	25	70 - 130	60 - 135

	13C12-2-MoCB	1L	100	50 - 145	70	20 - 135	15 - 145	5 - 145

13C12-4-MoCB	3L	100	50 - 145	70	20 - 135	15 - 145	5 - 145

13C12-2,2'-DiCB	4L	100	50 - 145	70	20 - 135	15 - 145	5 - 145

13C12-4,4'-DiCB	15L	100	50 - 145	70	20 - 135	15 - 145	5 - 145

13C12-2,2',6-TrCB	19L	100	50 - 145	70	20 - 135	15 - 145	5 - 145

13C12-3,4,4'-TrCB	37L	100	50 - 145	70	20 - 135	15 - 145	5 - 145

13C12-2,2',6,6'-TeCB	54L	100	50 - 145	70	20 - 135	15 - 145	5 - 145

13C12-3,3',4,4'-TeCB	77L	100	50 - 145	50	45 - 135	40 - 145	10 - 145

13C12-3,4,4',5-TeCB	81L	100	50 - 145	50	45 - 135	40 - 145	10 - 145

13C12-2,2',4,6,6'-PeCB	104L	100	50 - 145	50	45 - 135	40 - 145	10 - 145

13C12-2,3,3',4,4'-PeCB	105L	100	50 - 145	50	45 - 135	40 - 145	10 - 145

13C12-2,3,4,4',5-PeCB	114L	100	50 - 145	50	45 - 135	40 - 145	10 - 145

13C12-2,3',4,4',5-PeCB	118L	100	50 - 145	50	45 - 135	40 - 145	10 - 145

13C12-2',3,4,4',5-PeCB	123L	100	50 - 145	50	45 - 135	40 - 145	10 - 145

13C12-3,3',4,4',5-PeCB	126L	100	50 - 145	50	45 - 135	40 - 145	10 - 145

13C12-2,2',4,4',6,6'-HxCB	155L	100	50 - 145	50	45 - 135	40 - 145	10 -
145

13C12-2,3,3',4,4',5 -HxCB 6	156L	100	50 - 145	50	45 - 135	40 - 145	10 -
145

13C12-2,3,3',4,4',5'-HxCB 6	157L	100	50 - 145	50	45 - 135	40 - 145	10 -
145

13C12-2,3',4,4',5,5'-HxCB	167L	100	50 - 145	50	45 - 135	40 - 145	10 -
145

13C12-3,3',4,4',5,5'-HxCB	169L	100	50 - 145	50	45 - 135	40 - 145	10 -
145

13C12-2,2',3,4',5,6,6'-HpCB	188L	100	50 - 145	50	45 - 135	40 - 145	10 -
145

13C12-2',3,3',4,4',5,5'-HpCB	189L	100	50 - 145	50	45 - 135	40 - 145	10 -
145

13C12-2,2',3,3',5,5',6,6'-OcCB	202L	100	50 - 145	50	45 - 135	40 - 145	10
- 145

13C12-2,3,3',4,4',5,5',6-OcCB	205L	100	50 - 145	50	45 - 135	40 - 145	10
- 145

13C12-2,2',3,3',4,4',5,5',6-NoCB	206L	100	50 - 145	50	45 - 135	40 - 145
10 - 145

13C12-2,2',3,3',4,5,5',6,6'-NoCB	208L	100	50 - 145	50	45 - 135	40 - 145
10 - 145

13C12-2,2',3,3',4,4',5,5',6,6'-DeCB	209L	100	50 - 145	50	45 - 135	40 -
145	10 - 145

Cleanup standards

13C12-2,4,4'-TrCB	28L	100	65 - 135	70	20 - 135	15 - 145	5 - 145

13C12-2,3,3',5,5'-PeCB	111L	100	75 - 125	50	45 - 135	40 - 145	10 - 145

13C12-2,2',3,3',5,5',6-HpCB	178L	100	75 - 125	50	45 - 135	40 - 145	10 -
145

1.	Reference 22 describes how interlaboratory results were pooled from
analyses of wastewater, biosolids, and fish tissue samples.

2.	QC acceptance criteria for IPR, OPR, and samples based on a 20-µL
extract final volume

3.	Suffix “L” indicates labeled compound.

4.	See Table 5.

5.	Section 15.3.

6.	CBs 156/157 and 156L/157L are tested as the sum of the two congeners

	NA = Not applicable

Table 7.	Scan Descriptors, Levels of Chlorination, m/z Information, and
Substances Monitored by HRGC/HRMS

Function and Chlorine Level	 m/z1	m/z Type	m/z Formula	Substance

Fn-1; Cl-1	188.0393	M	12C12 H9 35Cl	Cl-1 CB

	 190.0363	M+2	12C12 H9 37Cl	Cl-1 CB

	 200.0795	M	13C12 H9 35Cl	13C12  Cl-1 CB

	 202.0766	M+2	13C12 H9 37Cl	13C12  Cl-1 CB

	 218.9856	lock	C4 F9	PFK

Fn-2; Cl-2, 3	 222.0003	M	12C12 H8 35Cl2	Cl-2 PCB

	 223.9974 (2)	M+2	12C12 H8 35Cl 37 Cl	Cl-2 PCB

	 225.9944	M+4	12C12 H8 37Cl2	Cl-2 PCB

	 234.0406	M	13C12 H8 35Cl2	13C12  Cl-2 PCB

	 236.0376	M+2	13C12 H8 35Cl 37 Cl	13C12  Cl-2 PCB

	 242.9856	lock	C6 F9	PFK

	 255.9613	M	12C12 H7 35Cl3	Cl-3 PCB

	 257.9584	M+2	12C12 H7 35Cl2 37Cl	Cl-3 PCB

	 268.0016	M	13C12 H7 35Cl3	13C12  Cl-3 PCB

	 269.9986	M+2	13C12 H7 35Cl2 37Cl	13C12  Cl-3 PCB

Fn-3; Cl-3, 4, 5	 255.9613	M	12C12 H7 35Cl3	Cl-3 PCB

	 257.9584	M+2	12C12 H7 35Cl2 37Cl	Cl-3 PCB

	 259.9554	M+4	12C12 H7 35Cl 37Cl2	Cl-3 PCB

	 268.0016	M	13C12 H7 35Cl3	13C12  Cl-3 PCB

	 269.9986	M+2	13C12 H7 35Cl2 37Cl	13C12  Cl-3 PCB

	 280.9825	lock	C6 F11	PFK

	 289.9224	M	12C12 H6 35Cl4	Cl-4 PCB

	 291.9194	M+2	12C12 H6 35Cl3 37Cl	Cl-4 PCB

	 293.9165	M+4	12C12 H6 35Cl2 37Cl2	Cl-4 PCB

	 301.9626	M	13C12 H6 35Cl4	13C12  Cl-4 PCB

	 303.9597	M+2	13C12 H6 35Cl3 37Cl	13C12  Cl-4 PCB

	 323.8834	M	13C12 H5 35Cl5	Cl-5 PCB

	 325.8804	M+2	12C12 H5 35Cl4 37Cl	Cl-5 PCB

	 327.8775	M+4	12C12 H5 35Cl3 37Cl2	Cl-5 PCB

	 337.9207	M+2	13C12 H5 35Cl4 37Cl	13C12  Cl-5 PCB

	 339.9178	M+4	13C12 H5 35Cl3 37Cl2	13C12  Cl-5 PCB

Fn-4; Cl-4, 5, 6	 289.9224	M	12C12 H6 35Cl4	Cl-4 PCB

	 291.9194	M+2	12C12 H6 35Cl3 37Cl	Cl-4 PCB

	 293.9165	M+4	12C12 H6 35Cl2 37Cl2	Cl-4 PCB

	 301.9626	M	13C12 H6 35Cl3 37Cl	13C12  Cl-4 PCB

	 303.9597	M+2	13C12 H6 35Cl2 37Cl2	13C12  Cl-4 PCB

	 323.8834	M	12C12 H5 35Cl5	Cl-5 PCB

	 325.8804	M+2	12C12 H5 35Cl4 37Cl	Cl-5 PCB

	 327.8775	M+4	12C12 H5 35Cl3 37Cl2	Cl-5 PCB

	 330.9792	lock	C7 F15	PFK

	 337.9207	M+2	13C12 H5 35Cl4 37Cl	13C12  Cl-5 PCB

	 339.9178	M+4	13C12 H5 35Cl3 37Cl2	13C12  Cl-5 PCB

Fn-4; Cl-4, 5, 6	 359.8415	M+2	12C12 H4 35Cl5 37Cl	Cl-6 PCB

	 361.8385	M+4	12C12 H4 35Cl4 37Cl2	Cl-6 PCB

	 363.8356	M+6	12C12 H4 35Cl3 37Cl3	Cl-6 PCB

	 371.8817	M+2	13C12 H4 35Cl5 37Cl	13C12  Cl-6 PCB

	 373.8788	M+4	13C12 H4 35Cl4 37Cl2	13C12  Cl-6 PCB

Fn-5; Cl-5, 6, 7	 323.8834	M	12C12 H5 35Cl5	Cl-5 PCB

	 325.8804	M+2	12C12 H5 35Cl4 37Cl	Cl-5 PCB

	 327.8775	M+4	12C12 H5 35Cl3 37Cl2	Cl-5 PCB

	 337.9207	M+2	13C12 H5 35Cl4 37Cl	13C12  Cl-5 PCB

	 339.9178	M+4	13C12 H5 35Cl3 37Cl2	13C12  Cl-5 PCB

	 354.9792	lock	C9 F13	PFK

	 359.8415	M+2	12C12 H4 35Cl5 37Cl	Cl-6 PCB

	 361.8385	M+4	12C12 H4 35Cl4 37Cl2	Cl-6 PCB

	 363.8356	M+6	12C12 H4 35Cl3 37Cl3	Cl-6 PCB

	 371.8817	M+2	13C12 H4 35Cl5 37Cl	13C12  Cl-6 PCB

	 373.8788	M+4	13C12 H4 35Cl4 37Cl2	13C12  Cl-6 PCB

	 393.8025	M+2	12C12 H3 35Cl6 37Cl	Cl-7 PCB

	 395.7995	M+4	12C12 H3 35Cl5 37Cl2	Cl-7 PCB

	 397.7966	M+6	12C12 H3 35Cl4 37Cl3	Cl-7 PCB

	 405.8428	M+2	13C12 H3 35Cl6 37Cl	13C12  Cl-7 PCB

	 407.8398	M+4	13C12 H3 35Cl5 37Cl2	13C12  Cl-7 PCB

	 454.9728	QC	C11 F17	PFK

Fn-6; Cl-7, 8, 9, 10	 393.8025	M+2	12C12 H3 35Cl6 37Cl	Cl-7 PCB

	 395.7995	M+4	12C12 H3 35Cl5 37Cl2	Cl-7 PCB

	 397.7966	M+6	12C12 H3 35Cl4 37Cl3	Cl-7 PCB

	 405.8428	M+2	13C12 H3 35Cl6 37Cl	13C12  Cl-7 PCB

	 407.8398	M+4	13C12 H3 35Cl5 37Cl2	13C12  Cl-7 PCB

	 427.7635	M+2	12C12 H2 35Cl7 37Cl	Cl-8 PCB

	 429.7606	M+4	12C12 H2 35Cl6 37Cl2	Cl-8 PCB

	 431.7576	M+6	12C12 H2 35Cl5 37Cl3	Cl-8 PCB

	 439.8038	M+2	13C12 H2 35Cl7 37Cl	13C12  Cl-8 PCB

	 441.8008	M+4	13C12 H2 35Cl6 37Cl2	13C12  Cl-8 PCB

	 442.9728	QC	C10 F13	PFK

	 454.9728	lock	C11 F13	PFK

	 461.7246	M+2	12C12 H1 35Cl8 37Cl	Cl-9 PCB

	 463.7216	M+4	12C12 H1 35Cl7 37Cl2	Cl-9 PCB

	 465.7187	M+6	12C12 H1 35Cl6 37Cl3	Cl-9 PCB

	 473.7648	M+2	13C12 H1 35Cl8 37Cl	13C12  Cl-9 PCB

	 475.7619	M+4	13C12 H1 35Cl7 37Cl2	13C12  Cl-9 PCB

	 495.6856	M+2	12C12 H4 35Cl9 37Cl	Cl-10 PCB

	 497.6826	M+4	12C12 35Cl8 37Cl2	Cl-10 PCB

	 499.6797	M+6	12C12 35Cl7 37Cl3	Cl-10 PCB

Fn-6; Cl-7, 8, 9, 10	 507.7258	M+2	13C12 35Cl9 37Cl	13C12  Cl-10 PCB

	 509.7229	M+4	13C12 35Cl8 37Cl2	13C12  Cl-10 PCB

	 511.7199	M+6	13C12 35Cl7 37Cl3	13C12  Cl-10 PCB

1.	Isotopic masses used for accurate mass calculation

	1H 	  1.0078

	12C	12.0000

	13C	13.0034

	35Cl	34.9689

	37Cl	36.9659

	19F	18.9984

2.	An interference with PFK m/z 223.9872 may preclude meeting 10:1 S/N
for the DiCB congeners at the CS-0.2 and CS-1 calibration levels
(Section 10.3.3 and Table 5).  If this interferences occurs, 10:1 S/N
must be met at the CS-2 level.  See the note at Section 10.2.1 for
information on how to minimize this interference.

Table 8.	Theoretical Ion Abundance Ratios and QC Limits

Chlorine Atoms	m/z’s Forming Ratio	Theoretical Ratio	Lower QC Limit
Upper QC Limit

1	M/(M+2)	3.13	2.66	3.60

2	M/(M+2)	1.56	1.33	1.79

3	M/(M+2)	1.04	0.88	1.20

4	M/(M+2)	0.77	0.65	0.89

5	(M+2)/(M+4)	1.55	1.32	1.78

6	(M+2)/(M+4)	1.24	1.05	1.43

7	(M+2)/(M+4)	1.05	0.89	1.21

8	(M+2)/(M+4)	0.89	0.76	1.02

9	(M+2)/(M+4)	0.77	0.65	0.89

10	(M+4)(M+6)	1.16	0.99	1.33

Table 9.	Suggested Sample Quantities to be Extracted for Various
Matrices1

Sample Matrix2	Example	Percent Solids	Phase	Quantity Extracted

Single-phase

Aqueous	Drinking water	<1	 – 3	1000 mL

	Groundwater

Treated wastewater

	Solid	Dry soil	>20	Solid	10 g

	Compost

Ash

	Organic	Waste solvent	<1	Organic	10 g

	Waste oil

Organic polymer

	Tissue	Fish	 – 	Organic	10 g

	Human adipose

	Multi-phase - Liquid/Solid

Aqueous/Solid	Wet soil	1-30	Solid	10 g

	Untreated effluent

Digested municipal sludge

Filter cake

Paper pulp

	Organic/solid	Industrial sludge	1-100	Both	10 g

	Oily waste

	Multi-phase - Liquid/Liquid

Aqueous/organic	In-process effluent	<1	Organic	10 g

	Untreated effluent

Drum waste

	Multi-phase - Liquid/Liquid/Solid

Aqueous/organic/solid	Untreated effluent	>1	Organic and solid	10 g

	Drum waste

1.	The quantity of sample to be extracted is adjusted to provide 10 g of
solids (dry weight).  One liter of aqueous samples containing one
percent solids will contain 10 grams of solids.  For aqueous samples
containing greater than one percent solids, a lesser volume is used so
that 10 grams of solids (dry weight) will be extracted.  Other sample
volumes may be used to meet project needs.

2.	The sample matrix may be amorphous for some samples.  In general,
when the CBs are in contact with a multi-phase system in which one of
the phases is water, they will be preferentially dispersed in or
adsorbed on the alternate phase because of their low solubility in
water.

3.	Aqueous samples are filtered after spiking with the labeled
compounds.  The filtrate and the materials trapped on the filter are
extracted separately, and the extracts are combined for cleanup and
analysis.

Figure 4.  Solid-phase

Extraction Apparatus

Figure 5.  Soxhlet/Dean Stark Extractor

24.0	Glossary

These definitions and purposes are specific to this method, but have
been conformed to common usage to the extent possible.

24.1	Units of weight and measure and their abbreviations

	24.1.1	Symbols

ºC	degrees Celsius

µL	microliter

µm	micrometer

<	less than

>	greater than

%	percent

24.1.2	Alphabetical abbreviations

cm	centimeter

g	gram

h	hour

ID	inside diameter

in.	inch

L	liter

M	molecular ion

m	meter

mg	milligram

min	minute

mL	milliliter

mm	millimeter

m/z	mass-to-charge ratio

N	normal; gram molecular weight of solute divided by hydrogen equivalent
of solute, per liter of solution

OD	outside diameter

pg	picogram

ppb	part-per-billion

ppm	part-per-million

ppq	part-per-quadrillion

ppt	part-per-trillion

psig	pound-per-square-inch gauge

v/v	volume per unit volume

w/v	weight per unit volume

24.2	Definitions and acronyms (in alphabetical order)

Analyte – A CB tested for by this method.  The analytes are listed in
Table 1.

Calibration standard (CAL) – A solution prepared from a secondary
standard and/or stock solutions and used to calibrate the response of
the HRGC/HRMS instrument.

Calibration verification standard (VER) – The mid-point calibration
standard (CS-3) that is used to verify calibration.  See Table 5.

CB – Chlorinated biphenyl congener.  One of the 209 individual
chlorinated biphenyl congeners determined using this method.  The 209
CBs are listed in Table 1.

CS-0.2, CS-1, CS-2, CS-3, CS-4, CS-5 – See Calibration standards and
Table 5

DeCB – Decachlorobiphenyl (PCB 209)

DiCB – Dichlorobiphenyl

Field blank – An aliquot of reagent water or other reference matrix
that is placed in a sample container in the laboratory or the field, and
treated as a sample in all respects, including exposure to sampling site
conditions, storage, preservation, and all analytical procedures.  The
purpose of the field blank is to determine if the field or sample
transporting procedures and environments have contaminated the sample.

GC – Gas chromatograph or gas chromatography

GPC – Gel permeation chromatograph or gel permeation chromatography

HpCB – Heptachlorobiphenyl

HPLC – High performance liquid chromatograph or high performance
liquid chromatography

HRGC – High resolution GC

HRMS – High resolution MS

HxCB – Hexachlorobiphenyl

Labeled injection internal standard – All five, or any one of the
five, 13C12-labeled CB congeners spiked into the concentrated extract
immediately prior to injection of an aliquot of the extract into the
HRGC/HRMS.  The five Labeled injection internal standards in this method
are CBs with congener numbers 9L, 52L, 101L, 138L, and 194L.

Internal standard – a labeled compound used as a reference for
quantitation of other labeled compounds and for quantitation of native
CB congeners other than the congener of which it is a labeled analog.
See Internal standard quantitation.

Internal standard quantitation – A means of determining the
concentration of (1) a naturally occurring (native) compound by
reference to a compound other than its labeled analog and (2) a labeled
compound by reference to another labeled compound

IPR – Initial precision and recovery; four aliquots of a reference
matrix spiked with the analytes of interest and labeled compounds and
analyzed to establish the ability of the laboratory to generate
acceptable precision and recovery.  An IPR is performed prior to the
first time this method is used and any time the method or
instrumentation is modified.

Isotope dilution quantitation – A means of determining a naturally
occurring (native) compound by reference to the same compound in which
one or more atoms has been isotopically enriched.  In this method, all
12 carbon atoms in the biphenyl molecule are enriched with carbon-13 to
produce 13C12- labeled analogs of the chlorinated biphenyls.  The
13C12-labeled CBs are spiked into each sample and allow identification
and correction of the concentration of the native compounds in the
analytical process.

K-D – Kuderna-Danish concentrator; a device used to concentrate the
analytes in a solvent

Laboratory blank – See Method blank

Laboratory control sample (LCS) – See Ongoing precision and recovery
standard (OPR)

Laboratory reagent blank – See Method blank

May – This action, activity, or procedural step is neither required
nor prohibited.

May not – This action, activity, or procedural step is prohibited.

Method blank – An aliquot of reagent water that is treated exactly as
a sample including exposure to all glassware, equipment, solvents,
reagents, internal standards, and surrogates that are used with samples.
 The method blank is used to determine if analytes or interferences are
present in the laboratory environment, the reagents, or the apparatus.

Method Detection Limit – The minimum concentration of a substance that
can be measured and reported with 99% confidence that the analyte
concentration is greater than zero (40 CFR 136, Appendix B)

Minimum level of quantitation (ML) – The lowest level at which the
entire analytical system must give a recognizable signal and acceptable
calibration point for the analyte.  The ML represents the lowest
concentration at which an analyte can be measured with a known level of
confidence.  It may be equivalent to the concentration of the lowest
calibration standard, assuming that all method-specified sample weights,
volumes, and cleanup procedures have been employed.  The ML is
calculated by multiplying the MDL (pooled or unpooled, as appropriate)
by 3.18 and rounding the result to the number nearest to 1, 2, or 5 x 10
n, where n is zero or an integer (see 68 FR 11790).

MoCB – Monochlorobiphenyl

MS – Mass spectrometer or mass spectrometry

Must – This action, activity, or procedural step is required.

NoCB – Nonachlorobiphenyl

OcCB – Octachlorobiphenyl

OPR – Ongoing precision and recovery standard (OPR); a method blank
spiked with known quantities of analytes.  The OPR is analyzed exactly
like a sample.  Its purpose is to assure that the results produced by
the laboratory remain within the limits specified in this method for
precision and recovery.

Perfluorokerosene (PFK) – A mixture of compounds used to calibrate the
exact m/z scale in the HRMS

Preparation blank – See Method blank

Quality control check sample (QCS) – A sample containing all or a
subset of the analytes at known concentrations.  The QCS is obtained
from a source external to the laboratory or is prepared from a source of
standards different from the source of calibration standards.  It is
used to check laboratory performance with test materials prepared
external to the normal preparation process.

PeCB – Pentachlorobiphenyl

PCB – Polychlorinated biphenyl

Reagent water – Water demonstrated to be free from the analytes of
interest and potentially interfering substances at the method detection
limit for the analyte.

Relative standard deviation (RSD) – The standard deviation times 100
divided by the mean.  Also termed “coefficient of variation.”

RF – Response factor.  See Section 10.5.

RR – Relative response.  See Section 10.4.

SDS – Soxhlet/Dean-Stark extractor; an extraction device applied to
the extraction of solid and semi-solid materials (Reference 11 and
Figure 5)

Signal-to-noise ratio (S/N) – The height of the signal as measured
from the mean (average) of the noise to the peak maximum divided by the
width of the noise

Should – This action, activity, or procedural step is suggested but
not required.

SICP – Selected ion current profile; the line described by the signal
at an exact m/z

SPE – Solid-phase extraction; an extraction technique in which an
analyte is extracted from an aqueous sample by passage over or through a
material capable of reversibly adsorbing the analyte.  Also termed
liquid-solid extraction.

Stock solution – A solution containing an analyte that is prepared
using a reference material traceable to EPA, the National Institute of
Science and Technology (NIST), or a source that will attest to the
purity and authenticity of the reference material.

TeCB – Tetrachlorobiphenyl

TEF – Toxicity equivalency factor; an estimate of the toxicity of a
specific congener relative to 2,3,7,8-tetrachlorodibenzo-p-dioxin

TEQ – The toxicity equivalent concentration in an environmental
sample.  It is the sum of the concentrations of each individual toxic
PCB and each individual 2,3,7,8-substituted, tetra-through
octa-chlorinated, dibenzo-p-dioxin and dibenzofuran multiplied by their
respective TEFs (Reference 1).

TEQPCB – The portion of the TEQ attributable to the toxic PCBs

TrCB – Trichlorobiphenyl

Unique GC resolution or uniquely resolved – Two adjacent
chromatographic peaks in which the height of the valley is less than 40
percent of the height of the shorter peak. See Section <IP_ADDRESS>.2 and
Figures 6 and 7 for unique resolution specific to the SPB-octyl column.

VER – See Calibration verification

Appendix A   -	Preliminary Information for Determination of 209 CBs on
the DB-1 Column

1.0	Column and Conditions

1.1	Column – 30 ± 5-m long x 0.25 ± 0.02-mm ID; 0.25 µm film DB-1
(J&W, or equivalent).

1.2	Suggested GC operating conditions:

Injector temperature:	270 ºC

Interface temperature:	290 ºC

Initial temperature:	75 ºC

Initial time:	2 minutes

Temperature program:	75-150 ºC at 15 ºC/minute

	150-270 ºC at 2.5 ºC/minute

Final time:	7 minutes

Carrier gas velocity:	40 cm/sec at 200 ºC

Note:	The GC conditions may be optimized for compound separation and
sensitivity.  Once optimized, the same GC conditions must be used for
the analysis of all standards, blanks, IPR and OPR aliquots, and
samples. ADVANCE \d7

2.0	Operating Information

2.1	Congener solutions – Mixes of individual congeners that will allow
separation of all 209 congeners on the DB-1 column had not been
developed when writing Method 1668C.

2.2	Elution order data – The congener mixes developed for the
SPB-octyl column (Table 4 of Method 1668C) were run on the DB-1 column.
Although some congeners in these mixes co-elute, the mixes allow
determination of retention times for many congeners on the DB-1 column.
These retention times are shown in Appendix Table A-1.

2.3	Window-defining congeners – The beginning and ending congeners at
each level of chlorination are the same as for the SPB-octyl column.
See Table 2 in Method 1668C.

2.4	Scan descriptors – The 6-function scan descriptors are shown in
Appendix Table A-2.

Table A-1.	Retention time (RT) References, Quantitation References, and
Relative Retention Times (RRTs) for CB Congeners using a

DB-1 Column

Labeled or Native CB1	Congener No.2	Retention Time and Quantitation
References	Congener No.2	RT	RRT	RRT QC Limits3

13C12-2-MoCB4	1L	13C12-4-MoCB4,5	3L	09:17	0.8855	0.8776-0.8935

2-MoCB	1	13C12-2-MoCB4	1L	09:17	1.0000	0.9964-1.0072

3-MoCB	2	13C12-4-MoCB4,5	3L	10:22	0.9889	0.9809-0.9968

13C12-4-MoCB4,5	3L	13C12-2,2',5,5'-TeCB7	52L	10:29	0.5561	0.5473-0.5650

4-MoCB	3	13C12-4-MoCB4,5	3L	10:29	1.0000	0.9968-1.0064

13C12-2,2'-DiCB4	4L	13C12-4,4'-DiCB4,5	15L	11:08	0.7591	0.7477-0.7705

2,2'-DiCB	4	13C12-2,2'-DiCB4	4L	11:08	1.0000	0.9925-1.0075

2,6-DiCB	10	13C12-4,4'-DiCB4,5	15L	11:10	0.7614	0.7500-0.7727

2,5-DiCB	9	13C12-4,4'-DiCB4,5	15L	12:08	0.8273	0.8216-0.8330

2,4-DiCB	7	13C12-4,4'-DiCB4,5	15L	12:09	0.8284	0.8227-0.8341

2,3'-DiCB	6	13C12-4,4'-DiCB4,5	15L	12:31	0.8534	0.8477-0.8591

2,4'-DiCB6	8	13C12-4,4'-DiCB4,5	15L	12:43	0.8670	0.8614-0.8727

2,3-DiCB	5	13C12-4,4'-DiCB4,5	15L	12:46	0.8705	0.8648-0.8761

13C12-2,2',6-TrCB4	19L	13C12-2,4,4'-TrCB5	28L	13:31	0.7990	0.7892-0.8089

3,5-DiCB	14	13C12-4,4'-DiCB4,5	15L	13:36	0.9273	0.9216-0.9330

 2,4,6-TrCB	30	13C12-2,4,4'-TrCB5	28L	14:06	0.8335	0.8286-0.8384

3,3'-DiCB	11	13C12-4,4'-DiCB4,5	15L	14:11	0.9670	0.9614-0.9727

3,4'-DiCB	13	13C12-4,4'-DiCB4,5	15L	14:26	0.9841	0.9784-0.9898

3,4-DiCB	12	13C12-4,4'-DiCB4,5	15L	14:27	0.9852	0.9795-0.9909

2,2',5-TrCB6	18	13C12-2,4,4'-TrCB5	28L	14:36	0.8631	0.8581-0.8680

13C12-4,4'-DiCB4,5	15L	13C12-2,2',5,5'-TeCB7	52L	14:40	0.7781
0.7692-0.7869

4,4'-DiCB	15	13C12-4,4'-DiCB4,5	15L	14:40	1.0000	0.9977-1.0043

2,2',4-TrCB	17	13C12-2,4,4'-TrCB5	28L	14:43	0.8700	0.8650-0.8749

 2,3',6-TrCB	27	13C12-2,4,4'-TrCB5	28L	15:06	0.8926	0.8877-0.8975

2,3,6-TrCB	24	13C12-2,4,4'-TrCB5	28L	15:06	0.8926	0.8877-0.8975

2,2',3-TrCB	16	13C12-2,4,4'-TrCB5	28L	15:26	0.9123	0.9074-0.9172

 2,4',6-TrCB	32	13C12-2,4,4'-TrCB5	28L	15:29	0.9153	0.9103-0.9202

13C12-2,2',6,6'-TeCB4	54L	13C12-3,3',4,4'-TeCB4,5,9	77L	16:02	0.6139
0.6075-0.6203

2,2',6,6'-TeCB	54	13C12-2,2',6,6'-TeCB4	54L	16:02	1.0000	0.9979-1.0042

2',3,5-TrCB	34	13C12-2,4,4'-TrCB5	28L	16:03	0.9488	0.9438-0.9537

2,3,5-TrCB	23	13C12-2,4,4'-TrCB5	28L	16:07	0.9527	0.9478-0.9576

2,4,5-TrCB	29	13C12-2,4,4'-TrCB5	28L	16:18	0.9635	0.9586-0.9685

2,3',5-TrCB	26	13C12-2,4,4'-TrCB5	28L	16:29	0.9744	0.9695-0.9793

2,3',4-TrCB	25	13C12-2,4,4'-TrCB5	28L	16:36	0.9813	0.9764-0.9862

2,4',5-TrCB	31	13C12-2,4,4'-TrCB5	28L	16:52	0.9970	0.9921-1.0020

13C12-2,4,4'-TrCB5	28L	13C12-2,2',5,5'-TeCB7	52L	16:55	0.8974
0.8930-0.9019

 2,4,4'-TrCB6	28	13C12-2,4,4'-TrCB5	28L	16:55	1.0000	0.9980-1.0039

2,2',4,6-TeCB	50	13C12-3,3',4,4'-TeCB4,5,9	77L	16:55	0.6477
0.6414-0.6541

 2,3,4-TrCB	21	13C12-2,4,4'-TrCB5	28L	17:21	1.0256	1.0207-1.0305

2,2',5,6'-TeCB	53	13C12-3,3',4,4'-TeCB4,5,9	77L	17:26	0.6675
0.6611-0.6739

2,3,3'-TrCB	20	13C12-2,4,4'-TrCB5	28L	17:22	1.0266	1.0217-1.0315

2',3,4-TrCB	33	13C12-2,4,4'-TrCB5	28L	17:24	1.0286	1.0236-1.0335

2,2',4,6'-TeCB	51	13C12-3,3',4,4'-TeCB4,5,9	77L	17:42	0.6777
0.6713-0.6841

2,3,4'-TrCB	22	13C12-2,4,4'-TrCB5	28L	17:43	1.0473	1.0424-1.0522

2,2',3,6-TeCB	45	13C12-3,3',4,4'-TeCB4,5,9	77L	18:00	0.6892
0.6828-0.6956

3,3',5-TrCB	36	13C12-2,4,4'-TrCB5	28L	18:16	1.0798	1.0749-1.0847

2,2',3,6'-TeCB	46	13C12-3,3',4,4'-TeCB4,5,9	77L	18:24	0.7045
0.6981-0.7109

3,4',5-TrCB	39	13C12-2,4,4'-TrCB5	28L	18:37	1.1005	1.0956-1.1054

13C12-2,2',5,5'-TeCB7	52L	13C12-2,2',5,5'-TeCB7	52L	18:51	1.0000
0.9956-1.0044

2,2',5,5'-TeCB6	52	13C12-3,3',4,4'-TeCB4,5,9	77L	18:51	0.7218
0.7154-0.7281

 2,3',4,6-TeCB	69	13C12-3,3',4,4'-TeCB4,5,9	77L	18:52	0.7224
0.7160-0.7288

 2,3',5',6-TeCB	73	13C12-3,3',4,4'-TeCB4,5,9	77L	18:57	0.7256
0.7192-0.7320

2,2',4,5'-TeCB	49	13C12-3,3',4,4'-TeCB4,5,9	77L	19:00	0.7275
0.7211-0.7339

 2,2',3,5-TeCB	43	13C12-3,3',4,4'-TeCB4,5,9	77L	19:04	0.7301
0.7237-0.7364

3,4,5-TrCB	38	13C12-2,4,4'-TrCB5	28L	19:12	1.1350	1.1300-1.1399

2,2',4,4'-TeCB	47	13C12-3,3',4,4'-TeCB4,5,9	77L	19:15	0.7371
0.7307-0.7435

2,4,4',6-TeCB	75	13C12-3,3',4,4'-TeCB4,5,9	77L	19:20	0.7403
0.7339-0.7466

2,2',4,5-TeCB	48	13C12-3,3',4,4'-TeCB4,5,9	77L	19:20	0.7403
0.7339-0.7466

2,3,5,6-TeCB	65	13C12-3,3',4,4'-TeCB4,5,9	77L	19:31	0.7473	0.7409-0.7537

 2,3,4,6-TeCB	62	13C12-3,3',4,4'-TeCB4,5,9	77L	19:36	0.7505
0.7441-0.7569

3,3',4-TrCB	35	13C12-2,4,4'-TrCB5	28L	19:41	1.1635	1.1586-1.1685

13C12-2,2',4,6,6'-PeCB4	104L	13C12-2,3',4,4',5-PeCB5,9	118L	19:45	0.7037
0.6977-0.7096

2,2',4,6,6'-PeCB	104	13C12-2,2',4,6,6'-PeCB4	104L	19:45	1.0000
0.9983-1.0034

2,2',3,5'-TeCB6	44	13C12-3,3',4,4'-TeCB4,5,9	77L	19:55	0.7626
0.7562-0.7690

13C12-3,4,4'-TrCB4	37L	13C12-2,4,4'-TrCB5	28L	20:03	1.1852	1.1803-1.1901

3,4,4'-TrCB	37	13C12-3,4,4'-TrCB4	37L	20:03	1.0000	0.9983-1.0033

2,3,3',6-TeCB	59	13C12-3,3',4,4'-TeCB4,5,9	77L	20:05	0.7690
0.7626-0.7754

2,2',3,4'-TeCB	42	13C12-3,3',4,4'-TeCB4,5,9	77L	20:07	0.7703
0.7639-0.7766

 2,3',5,5'-TeCB	72	13C12-3,3',4,4'-TeCB4,5,9	77L	20:36	0.7888
0.7824-0.7951

 2,3',4',6-TeCB	71	13C12-3,3',4,4'-TeCB4,5,9	77L	20:36	0.7888
0.7824-0.7951

2,3,4',6-TeCB	64	13C12-3,3',4,4'-TeCB4,5,9	77L	20:37	0.7894
0.7830-0.7958

 2,2',3,4-TeCB	41	13C12-3,3',4,4'-TeCB4,5,9	77L	20:39	0.7907
0.7843-0.7971

2,2',3,6,6'-PeCB	96	13C12-2,3',4,4',5-PeCB5,9	118L	20:48	0.7411
0.7352-0.7470

 2,3',4,5'-TeCB	68	13C12-3,3',4,4'-TeCB4,5,9	77L	20:52	0.7990
0.7926-0.8054

2,2',3,3'-TeCB	40	13C12-3,3',4,4'-TeCB4,5,9	77L	20:58	0.8028
0.7996-0.8060

2,3,3',5-TeCB	57	13C12-3,3',4,4'-TeCB4,5,9	77L	21:21	0.8175
0.8143-0.8207

2,2',4,5,'6-PeCB	103	13C12-2,3',4,4',5-PeCB5,9	118L	21:22	0.7613
0.7553-0.7672

 2,3',4,5-TeCB	67	13C12-3,3',4,4'-TeCB4,5,9	77L	21:38	0.8283
0.8251-0.8315

2,2',4,4',6-PeCB	100	13C12-2,3',4,4',5-PeCB5,9	118L	21:41	0.7726
0.7666-0.7785

2,3,3',5'-TeCB	58	13C12-3,3',4,4'-TeCB4,5,9	77L	21:43	0.8315
0.8283-0.8347

 2,3,4',5-TeCB	63	13C12-3,3',4,4'-TeCB4,5,9	77L	21:51	0.8366
0.8334-0.8398

 2,2',3,5,6'-PeCB	94	13C12-2,3',4,4',5-PeCB5,9	118L	22:05	0.7868
0.7809-0.7928

2,4,4',5-TeCB	74	13C12-3,3',4,4'-TeCB4,5,9	77L	22:07	0.8468
0.8437-0.8500

 2,3,4,5-TeCB	61	13C12-3,3',4,4'-TeCB4,5,9	77L	22:11	0.8494
0.8462-0.8526

2,3',4',5-TeCB	70	13C12-3,3',4,4'-TeCB4,5,9	77L	22:20	0.8551
0.8519-0.8583

 2',3,4,5-TeCB	76	13C12-3,3',4,4'-TeCB4,5,9	77L	22:25	0.8583
0.8551-0.8615

 2,2',3',4,6-PeCB	98	13C12-2,3',4,4',5-PeCB5,9	118L	22:28	0.8005
0.7975-0.8034

2,3',4,4'-TeCB6	66	13C12-3,3',4,4'-TeCB4,5,9	77L	22:29	0.8609
0.8577-0.8641

2,2',4,5,6'-PeCB	102	13C12-2,3',4,4',5-PeCB5,9	118L	22:32	0.8029
0.7999-0.8058

2,2',3,5',6-PeCB	95	13C12-2,3',4,4',5-PeCB5,9	118L	22:34	0.8040
0.8011-0.8070

 2,2',3,5,6-PeCB	93	13C12-2,3',4,4',5-PeCB5,9	118L	22:36	0.8052
0.8023-0.8082

3,3',5,5'-TeCB	80	13C12-3,3',4,4'-TeCB4,5,9	77L	22:45	0.8711
0.8679-0.8743

2,2',3,4,6-PeCB	88	13C12-2,3',4,4',5-PeCB5,9	118L	22:49	0.8129
0.8100-0.8159

2,2',3,4',6-PeCB	91	13C12-2,3',4,4',5-PeCB5,9	118L	22:55	0.8165
0.8135-0.8195

2,3,3',4'-TeCB	55	13C12-3,3',4,4'-TeCB4,5,9	77L	22:57	0.8787
0.8756-0.8819

2,3',4,5,'6-PeCB	121	13C12-2,3',4,4',5-PeCB5,9	118L	23:04	0.8219
0.8189-0.8248

2,3,3',4'-TeCB	56	13C12-3,3',4,4'-TeCB4,5,9	77L	23:24	0.8960
0.8928-0.8992

 2,3,4,4'-TeCB	60	13C12-3,3',4,4'-TeCB4,5,9	77L	23:24	0.8960
0.8928-0.8992

13C12-2,2',4,4',6,6'-HxCB4	155L	13C12-2,3',4,4',5,5'-HxCB5,9	167L	23:43
0.7104	0.7054-0.7154

2,2',4,4',6,6'-HxCB	155	13C12-2,2',4,4',6,6'-HxCB4	155L	23:43	1.0000
0.9986-1.0028

2,2',3,3',6-PeCB	84	13C12-2,3',4,4',5-PeCB5,9	118L	23:44	0.8456
0.8426-0.8486

 2,2',3,5,5'-PeCB	92	13C12-2,3',4,4',5-PeCB5,9	118L	23:50	0.8492
0.8462-0.8521

  2,2',3,4,6'-PeCB	89	13C12-2,3',4,4',5-PeCB5,9	118L	23:53	0.8510
0.8480-0.8539

2,2',3,4',5-PeCB	90	13C12-2,3',4,4',5-PeCB5,9	118L	24:07	0.8593
0.8563-0.8622

13C12-2,2',4,5,5'-PeCB7	101L	13C12-2,2',4,5,5'-PeCB7	101L	24:11	1.0000
0.9966-1.0034

2,2',4,5,5'-PeCB6	101	13C12-2,3',4,4',5-PeCB5,9	118L	24:11	0.8616
0.8587-0.8646

2,3,3',5',6-PeCB	113	13C12-2,3',4,4',5-PeCB5,9	118L	24:23	0.8688
0.8658-0.8717

3,3',4,5'-TeCB	79	13C12-3,3',4,4'-TeCB4,5,9	77L	24:27	0.9362
0.9330-0.9394

2,2',4,4',5-PeCB	99	13C12-2,3',4,4',5-PeCB5,9	118L	24:28	0.8717
0.8688-0.8747

2,2',3,4',6,6'-HxCB	150	13C12-2,3',4,4',5,5'-HxCB5,9	167L	24:52	0.7449
0.7399-0.7499

2,3',4,4',6-PeCB	119	13C12-2,3',4,4',5-PeCB5,9	118L	24:54	0.8872
0.8842-0.8901

 2,3,3',5,6-PeCB	112	13C12-2,3',4,4',5-PeCB5,9	118L	25:00	0.8907
0.8878-0.8937

2,3,3',4,6-PeCB	109	13C12-2,3',4,4',5-PeCB5,9	118L	25:09	0.8961
0.8931-0.8990

2,2',3,5,6,6'-HxCB	152	13C12-2,3',4,4',5,5'-HxCB5,9	167L	25:17	0.7574
0.7524-0.7624

2,2',3,3',5-PeCB	83	13C12-2,3',4,4',5-PeCB5,9	118L	25:20	0.8919
0.8890-0.8949

2,2',3',4,5-PeCB	97	13C12-2,3',4,4',5-PeCB5,9	118L	25:22	0.9038
0.9008-0.9068

2,2',3,4,5-PeCB	86	13C12-2,3',4,4',5-PeCB5,9	118L	25:27	0.9068
0.9038-0.9097

13C12-3,4,4',5-TeCB9	81L	13C12-2,2',5,5'-TeCB7	52L	25:32	1.3546
1.3457-1.3634

3,4,4',5-TeCB10	81	13C12-3,4,4',5-TeCB4,5,9	77L	25:32	1.0000
0.9987-1.0026

 2',3,4,5,6'-PeCB	125	13C12-2,3',4,4',5-PeCB5,9	118L	25:36	0.9121
0.9091-0.9151

2,3,4',5,6-PeCB	117	13C12-2,3',4,4',5-PeCB5,9	118L	25:37	0.9127
0.9097-0.9157

2,2',3,4,5'-PeCB	87	13C12-2,3',4,4',5-PeCB5,9	118L	25:38	0.9133
0.9103-0.9163

3,3',4,5-TeCB	78	13C12-3,3',4,4'-TeCB4,5,9	77L	25:40	0.9598
0.9566-0.9630

2,2',3,4,6,6'-HxCB	145	13C12-2,3',4,4',5,5'-HxCB5,9	167L	25:42	0.7698
0.7649-0.7748

 2,3,4,4',6-PeCB	115	13C12-2,3',4,4',5-PeCB5,9	118L	25:44	0.9169
0.9139-0.9198

13C12-2,3,3',5,5'-PeCB8	111L	13C12-2,2',4,5,5'-PeCB7	101L	25:51	1.0689
1.0655-1.0724

 2,3,3',5,5'-PeCB	111	13C12-2,3',4,4',5-PeCB5,9	118L	25:51	0.9210
0.9181-0.9240

2,2',3,4,4'-PeCB	85	13C12-2,3',4,4',5-PeCB5,9	118L	25:51	0.9210
0.9181-0.9240

 2,3,4,5,6-PeCB	116	13C12-2,3',4,4',5-PeCB5,9	118L	25:48	0.9192
0.9163-0.9222

13C12-3,3',4,4'-TeCB4,5,9	77L	13C12-2,2',5,5'-TeCB7	52L	26:07	1.3855
1.3767-1.3943

3,3',4,4'-TeCB6,10	77	13C12-3,3',4,4'-TeCB4,5,9	77L	26:07	1.0000
0.9987-1.0026

2,2',3,3',6,6'-HxCB	136	13C12-2,3',4,4',5,5'-HxCB5,9	167L	26:10	0.7793
0.7743-0.7843

2,3',4,5,5'-PeCB	120	13C12-2,3',4,4',5-PeCB5,9	118L	26:12	0.9335
0.9305-0.9365

2,2',3,4',5,6'-HxCB	148	13C12-2,3',4,4',5,5'-HxCB5,9	167L	26:14	0.7858
0.7808-0.7908

2,3,3',4',6-PeCB	110	13C12-2,3',4,4',5-PeCB5,9	118L	26:16	0.9359
0.9329-0.9388

2,2',4,4',5,6'-HxCB	154	13C12-2,3',4,4',5,5'-HxCB5,9	167L	26:44	0.8008
0.7983-0.8033

2,2',3,3',4-PeCB	82	13C12-2,3',4,4',5-PeCB5,9	118L	26:48	0.9549
0.9519-0.9578

2,2',3,5,5',6-HxCB	151	13C12-2,3',4,4',5,5'-HxCB5,9	167L	27:18	0.8178
0.8153-0.8203

2,2',3,3',5,6'-HxCB	135	13C12-2,3',4,4',5,5'-HxCB5,9	167L	27:31	0.8243
0.8218-0.8268

 2',3,4,5,5'-PeCB	124	13C12-2,3',4,4',5-PeCB5,9	118L	27:36	0.9834
0.9804-0.9863

2,2',3,4,5',6-HxCB	144	13C12-2,3',4,4',5,5'-HxCB5,9	167L	27:38	0.8278
0.8253-0.8303

2,3,3',4,5'-PeCB	108	13C12-2,3',4,4',5-PeCB5,9	118L	27:40	0.9857
0.9828-0.9887

2,2',3,4',5,6-HxCB	147	13C12-2,3',4,4',5,5'-HxCB5,9	167L	27:44	0.8308
0.8283-0.8333

2,3,3',4',5-PeCB	107	13C12-2,3',4,4',5-PeCB5,9	118L	27:45	0.9887
0.9857-0.9917

2,2',3,4',5',6-HxCB	149	13C12-2,3',4,4',5,5'-HxCB5,9	167L	28:01	0.8392
0.8367-0.8417

2,2',3,3',5,6-HxCB	134	13C12-2,3',4,4',5,5'-HxCB5,9	167L	28:35	0.8562
0.8537-0.8587

 2,2',3,4,5,6'-HxCB	143	13C12-2,3',4,4',5,5'-HxCB5,9	167L	28:34	0.8557
0.8532-0.8582

13C12-2',3,4,4',5-PeCB9	123L	13C12-2,2',4,5,5'-PeCB7	101L	27:53	1.1530
1.1496-1.1564

2',3,4,4',5-PeCB10	123	13C12-2',3,4,4',5-PeCB9	123L	27:53	1.0000
0.9988-1.0024

 2,2',3,4,4',6-HxCB	139	13C12-2,3',4,4',5,5'-HxCB5,9	167L	28:01	0.8392
0.8367-0.8417

 2,3,3',4,5-PeCB	106	13C12-2,3',4,4',5-PeCB5,9	118L	28:04	1.0000
0.9970-1.0030

13C12-2,3',4,4',5-PeCB5,9	118L	13C12-2,2',4,5,5'-PeCB7	101L	28:04	1.1606
1.1571-1.1640

2,3',4,4',5-PeCB6,10	118	13C12-2,3',4,4',5-PeCB5,9	118L	28:04	1.0000
0.9988-1.0024

 2,2',3,4,4',6'-HxCB	140	13C12-2,3',4,4',5,5'-HxCB5,9	167L	28:12	0.8447
0.8422-0.8472

13C12-2,3,4,4',5-PeCB9	114L	13C12-2,2',4,5,5'-PeCB7	101L	28:38	1.1840
1.1806-1.1875

2,3,4,4',5-PeCB10	114	13C12-2,3,4,4',5-PeCB9	114L	28:38	1.0000
0.9988-1.0023

2',3,3',4,5-PeCB	122	13C12-2,3',4,4',5-PeCB5,9	118L	28:48	1.0261
1.0232-1.0291

2,2',3,3',4,6-HxCB	131	13C12-2,3',4,4',5,5'-HxCB5,9	167L	28:52	0.8647
0.8622-0.8672

2,2',3,4,5,6-HxCB	142	13C12-2,3',4,4',5,5'-HxCB5,9	167L	28:59	0.8682
0.8657-0.8707

2,2',3,3',5,5'-HxCB	133	13C12-2,3',4,4',5,5'-HxCB5,9	167L	28:59	0.8682
0.8657-0.8707

2,2',3,3',4,6'-HxCB	132	13C12-2,3',4,4',5,5'-HxCB5,9	167L	29:32	0.8847
0.8822-0.8872

 2,3,3',5,5',6-HxCB	165	13C12-2,3',4,4',5,5'-HxCB5,9	167L	29:21	0.8792
0.8767-0.8817

13C12-2,2',3,4',5,6,6'-HpCB4	188L	13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L
29:22	0.9511	0.7327-0.7411

2,2',3,4',5,6,6'-HpCB	188	13C12-2,2',3,4',5,6,6'-HpCB4	188L	29:22	1.0000
0.9989-1.0023

2,2',3,4',5,5'-HxCB	146	13C12-2,3',4,4',5,5'-HxCB5,9	167L	29:24	0.8807
0.8782-0.8832

13C12-2,3,3',4,4'-PeCB9	105L	13C12-2,2',4,5,5'-PeCB7	101L	29:30	1.2198
1.2130-1.2267

2,3,3',4,4'-PeCB6,10	105	13C12-2,3,3',4,4'-PeCB9	105L	29:30	1.0000
0.9989-1.0023

2,3,3',4,5',6-HxCB	161	13C12-2,3',4,4',5,5'-HxCB5,9	167L	29:32	0.8847
0.8822-0.8872

2,2',4,4',5,5'-HxCB6	153	13C12-2,3',4,4',5,5'-HxCB5,9	167L	29:48	0.8927
0.8902-0.8952

2,2',3,4,4',6,6'-HpCB	184	13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L	29:49
0.7482	0.7440-0.7524

3,3',4,5,5'-PeCB	127	13C12-2,3',4,4',5-PeCB5,9	118L	29:57	1.0671
1.0641-1.0701

2,3',4,4',5',6-HxCB	168	13C12-2,3',4,4',5,5'-HxCB5,9	167L	29:59	0.8982
0.8957-0.9006

2,2',3,4,5,5'-HxCB	141	13C12-2,3',4,4',5,5'-HxCB5,9	167L	30:31	0.9141
0.9116-0.9166

2,2',3,3',5,6,6'-HpCB	179	13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L	30:33
0.7666	0.7624-0.7708

2,2',3,4,4',5-HxCB	137	13C12-2,3',4,4',5,5'-HxCB5,9	167L	30:51	0.9241
0.9216-0.9266

2,2',3,3',4,5'-HxCB	130	13C12-2,3',4,4',5,5'-HxCB5,9	167L	30:57	0.9271
0.9246-0.9296

2,2',3,3',4,6,6'-HpCB	176	13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L	31:01
0.7783	0.7742-0.7825

13C12-2,2',3,4,4',5'-HxCB7	138L	13C12-2,2',3,4,4',5'-HxCB7	138L	31:20
1.0000	0.9973-1.0027

2,2',3,4,4',5'-HxCB6	138	13C12-2,3',4,4',5,5'-HxCB5,9	167L	31:20	0.9386
0.9361-0.9411

 2,3,3',4',5',6-HxCB	164	13C12-2,3',4,4',5,5'-HxCB5,9	167L	31:22	0.9396
0.9371-0.9421

 2,3,3',4',5,6-HxCB	163	13C12-2,3',4,4',5,5'-HxCB5,9	167L	31:28	0.9426
0.9401-0.9451

 2,3,3',4,5,6-HxCB	160	13C12-2,3',4,4',5,5'-HxCB5,9	167L	31:33	0.9451
0.9426-0.9476

2,3,3',4,4',6-HxCB	158	13C12-2,3',4,4',5,5'-HxCB5,9	167L	31:35	0.9461
0.9436-0.9486

2,2',3,4,5,6,6'-HpCB	186	13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L	31:36
0.7930	0.7888-0.7972

2,2',3,3',4,5-HxCB	129	13C12-2,3',4,4',5,5'-HxCB5,9	167L	31:48	0.9526
0.9501-0.9551

13C12-3,3',4,4',5-PeCB4,9	126L	13C12-2,2',4,5,5'-PeCB7	101L	31:49	1.3156
1.3088-1.3225

3,3',4,4',5-PeCB6,10	126	13C12-3,3',4,4',5-PeCB4,9	126L	31:49	1.0000
0.9990-1.0021

 2,3,4,4',5,6-HxCB	166	13C12-2,3',4,4',5,5'-HxCB5,9	167L	32:13	0.9651
0.9626-0.9675

13C12-2,2',3,3',5,5',6-HpCB7	178L	13C12-2,2',3,3',5,5',6-HpCB7	178L
32:14	1.0000	0.9974-1.0026

2,2',3,3',5,5',6-HpCB	178	13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L	32:14
0.8089	0.8068-0.8110

2,2',3,3',4,5',6-HpCB	175	13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L	32:33
0.8168	0.8147-0.8189

2,3,3',4,5,5'-HxCB	159	13C12-2,3',4,4',5,5'-HxCB5,9	167L	32:43	0.9800
0.9775-0.9825

2,2',3,4',5,5',6-HpCB6	187	13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L	32:46
0.8223	0.8202-0.8243

 2,2',3,4,4',5,6'-HpCB	182	13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L	32:47
0.8227	0.8206-0.8248

2,2',3,3',4,4'-HxCB6	128	13C12-2,3',4,4',5,5'-HxCB5,9	167L	32:52	0.9845
0.9820-0.9870

2,3,3',4',5,5'-HxCB	162	13C12-2,3',4,4',5,5'-HxCB5,9	167L	33:00	0.9885
0.9860-0.9910

2,2',3,4,4',5',6-HpCB	183	13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L	33:06
0.8306	0.8285-0.8327

13C12-2,3',4,4',5,5'-HxCB5,9	167L	13C12-2,2',3,4,4',5'-HxCB7	138L	33:23
1.0654	1.0628-1.0681

2,3',4,4',5,5'-HxCB10	167	13C12-2,3',4,4',5,5'-HxCB5,9	167L	33:23	1.0000
0.9990-1.0020

2,2',3,4,5,5',6-HpCB	185	13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L	33:43
0.8461	0.8440-0.8482

2,2',3,3',4,5,6'-HpCB	174	13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L	34:07
0.8561	0.8540-0.8582

 2,2',3,4,4',5,6-HpCB	181	13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L	34:11
0.8578	0.8557-0.8599

2,2',3,3',4',5,6-HpCB	177	13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L	34:22
0.8624	0.8603-0.8645

2,2'3,3',4,4',6-HpCB	171	13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L	34:40
0.8699	0.8678-0.8720

13C12-2,3,3',4,4',5 -HxCB9	156L	13C12-2,2',3,4,4',5'-HxCB7	138L	34:40
1.1064	1.1037-1.1090

2,3,3',4,4',5-HxCB10	156	13C12-2,3,3',4,4',5 -HxCB9	156L	34:40	1.0000
0.9990-1.0019

13C12-2,2',3,3',5,5',6,6'-OcCB4	202L	13C12-Cl8-PCB-1945	194L	34:56
0.8265	0.8245-0.8285

2,2',3,3',5,5',6,6'-OcCB	202	13C12-2,2',3,3',5,5',6,6'-OcCB4	202L	34:56
1.0000	0.9990-1.0019

13C12-2,3,3',4,4',5'-HxCB9	157L	13C12-2,2',3,4,4',5'-HxCB7	138L	34:57
1.1154	1.1128-1.1181

2,3,3',4,4',5'-HxCB10	157	13C12-2,3,3',4,4',5'-HxCB9	157L	34:57	1.0000
0.9990-1.0019

2,2',3,3',4,5,6-HpCB	173	13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L	35:04
0.8800	0.8779-0.8821

2,2',3,3',4,5',6,6'-OcCB	201	13C12-Cl8-PCB-1945	194L	35:25	0.8379
0.8360-0.8399

2,2',3,4,4',5,6,6'-OcCB	204	13C12-Cl8-PCB-1945	194L	35:36	0.8423
0.8403-0.8442

2,2',3,3',4,5,5'-HpCB	172	13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L	35:41
0.8954	0.8934-0.8975

2,3,3',4,5,5',6-HpCB	192	13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L	35:51
0.8996	0.8975-0.9017

2,2',3,3',4,4',6,6'-OcCB	197	13C12-Cl8-PCB-1945	194L	35:55	0.8498
0.8478-0.8517

2,2',3,4,4',5,5'-HpCB6	180	13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L	36:07
0.9063	0.9042-0.9084

2,3,3',4',5,5',6-HpCB	193	13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L	36:20
0.9118	0.9097-0.9138

2,3,3',4,4',5',6-HpCB	191	13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L	36:34
0.9176	0.9155-0.9197

2,2',3,3',4,5,6,6'-OcCB	200	13C12-Cl8-PCB-1945	194L	36:49	0.8711
0.8691-0.8730

13C12-3,3',4,4',5,5'-HxCB4,9	169L	13C12-2,2',3,4,4',5'-HxCB7	138L	37:19
1.1910	1.1883-1.1936

3,3',4,4',5,5'-HxCB6,10	169	13C12-3,3',4,4',5,5'-HxCB4,9	169L	37:19
1.0000	0.9991-1.0018

2,2',3,3',4,4',5-HpCB6	170	13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L	37:44
0.9469	0.9448-0.9490

2,3,3',4,4',5,6-HpCB	190	13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L	37:56
0.9519	0.9498-0.9540

2,2',3,3',4,5,5',6-OcCB	198	13C12-Cl8-PCB-1945	194L	38:34	0.9125
0.9105-0.9144

2,2',3,3',4,5,5',6'-OcCB	199	13C12-Cl8-PCB-1945	194L	38:43	0.9160
0.9140-0.9180

2,2',3,3',4,4',5,6'-OcCB	196	13C12-Cl8-PCB-1945	194L	39:05	0.9247
0.9227-0.9267

2,2',3,4,4',5,5',6-OcCB	203	13C12-Cl8-PCB-1945	194L	39:05	0.9247
0.9227-0.9267

13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L	13C12-2,2',3,3',5,5',6-HpCB7	178L
39:51	1.2363	1.2311-1.2415

2,3,3',4,4',5,5'-HpCB10	189	13C12-2',3,3',4,4',5,5'-HpCB4,5,9	189L	39:51
1.0000	0.9992-1.0017

2,2',3,3',4,4',5,6-OcCB6	195	13C12-Cl8-PCB-1945	194L	40:45	0.9641
0.9621-0.9661

13C12-2,2',3,3',4,5,5',6,6'-NoCB4	208L	13C12-Cl9-PCB-2064,5	206L	41:03
0.9149	0.9131-0.9168

2,2',3,3',4,5,5',6,6'-NoCB	208	13C12-2,2',3,3',4,5,5',6,6'-NoCB4	208L
41:03	1.0000	0.9992-1.0016

2,2',3,3',4,4',5,6,6'-NoCB	207	13C12-Cl9-PCB-2064,5	206L	41:32	0.9257
0.9238-0.9276

13C12-2,2',3,3',4,4',5,5'-OcCB5	194L	13C12-2,2',3,3',5,5',6-HpCB7	178L
42:16	1.3113	1.3061-1.3164

2,2',3,3',4,4',5,5'-OcCB	194	13C12-Cl8-PCB-1945	194L	42:16	1.0000
0.9992-1.0016

13C12-2,3,3',4,4',5,5',6-OcCB4	205L	13C12-Cl8-PCB-1945	194L	42:44	1.0110
1.0091-1.0130

2,3,3',4,4',5,5',6-OcCB	205	13C12-2,3,3',4,4',5,5',6-OcCB4	205L	42:44
1.0000	0.9992-1.0016

13C12-2,2',3,3',4,4',5,5',6-NoCB4,5	206L	13C12-2,2',3,3',5,5',6-HpCB7
178L	44:52	1.3919	1.3868-1.3971

2,2',3,3',4,4',5,5',6-NoCB6	206	13C12-Cl9-PCB-2064,5	206L	44:52	1.0000
0.9993-1.0015

13C12-2,2',3,3',4,4',5,5',6,6'-DeCB4,5	209L	13C12-2,2',3,3',5,5',6-HpCB7
178L	46:55	1.4555	1.4504-1.4607

2,2',3,3',4,4',5,5',6,6'-DeCB6	209	13C12-Cl10-PCB-2094,5	209L	46:55
1.0000	0.9993-1.0014

1.	Abbreviations for chlorination levels

MoCB

monochlorobiphenyl

HxCB

hexachlorobiphenyl

DiCB

dichlorobiphenyl

HpCB

heptachlorobiphenyl

TrCB

trichlorobiphenyl

OcCB

octachlorobiphenyl

TeCB

tetrachlorobiphenyl

NoCB

nonachlorobiphenyl

PeCB

pentachlorobiphenyl

DeCB

decachlorobiphenyl

	4.	Labeled level of chlorination (LOC) window-defining congener

5.	Labeled level of chlorination (LOC) quantitation congener

6.	National Oceanic and Atmospheric Administration (NOAA) congener of
interest

2.	Suffix “L” indicates labeled compound	7.	Instrument internal
standard

3.	For native CBs determined by isotope dilution quantitation, RRT QC
limits were constructed using -2 to +4 seconds around the retention time
for the labeled analog.  For native CBs determined by internal standard
quantitation, RRT QC limits were constructed using a ± 2 percent window
around the retention time for retention times in the range of 0.8-1.2
and a ± 4 percent window around the retention time for retention times
<0.8 and >1.2.  These windows may not be adequate for analyte
identification (See the note in Section 16.4)	8.	Clean-up standard

9.	Labeled internal standard for World Health Organization (WHO) toxic
congener

10.	WHO toxic congener

Table A-2.	Scan Descriptors, Levels of Chlorination, m/z Information,
and Substances Monitored by HRGC/HRMS

Function and Chlorine Level	 m/z	m/z Type	m/z Formula	Substance

Fn-1 Cl-1	188.0393	M	12C12 H9 35Cl	Cl-1 PCB

	190.0363	M+2	12C12 H9 37Cl	Cl-1P CB

	200.0795	M	13C12 H9 35Cl	13C12 Cl-1 PCB

	202.0766	M+2	13C12 H9 37Cl	13C12 Cl-1 PCB

	218.9856	lock	C4 F9	PFK

Fn-2 Cl-2,3	222.0003	M	12C12 H8 35Cl2	Cl-2 PCB

	223.9974	M+2	12C12 H8 35Cl 37 Cl	Cl-2 PCB

	225.9944	M+4	12C12 H8 37Cl2	Cl-2 PCB

	234.0406	M	13C12 H8 35Cl2	13C12 Cl-2 PCB

	236.0376	M+2	13C12 H8 35Cl 37 Cl	13C12 Cl-2 PCB

	242.9856	lock	C6 F9	PFK

	255.9613	M	12C12 H7 35Cl3	Cl-3 PCB

	257.9584	M+2	12C12 H7 35Cl2 37Cl	Cl-3 PCB

Fn-3 Cl-3,4,5	255.9613	M	12C12 H7 35Cl3	Cl-3 PCB

	257.9584	M+2	12C12 H7 35Cl2 37Cl	Cl-3 PCB

	259.9554	M+4	12C12 H7 35Cl 37Cl2	Cl-3 PCB

	268.0016	M	13C12 H7 35Cl3	13C12 Cl-3 PCB

	269.9986	M+2	13C12 H7 35Cl2 37Cl	13C12 Cl-3 PCB

	280.9825	lock	C6 F11	PFK

	289.9224	M	12C12 H6 35Cl4	Cl-4 PCB

	291.9194	M+2	12C12 H6 35Cl3 37Cl	Cl-4 PCB

	293.9165	M+4	12C12 H6 35Cl2 37Cl2	Cl-4 PCB

	301.9626	M	13C12 H6 35Cl4	13C12 Cl-4 PCB

	303.9597	M+2	13C12 H6 35Cl3 37Cl	13C12 Cl-4 PCB

	323.8834	M	12C12 H5 35Cl5	Cl-5 PCB

	325.8804	M+2	12C12 H5 35Cl4 37Cl	Cl-5 PCB

	327.8775	M+4	12C12 H5 35Cl3 37Cl2	Cl-5 PCB

	337.9207	M+2	13C12 H5 35Cl4 37Cl	13C12 Cl-5 PCB

	339.9178	M+4	13C12 H5 35Cl3 37Cl2	13C12 Cl-5 PCB

Fn-4 Cl-4,5,6	289.9224	M	12C12 H6 35Cl4	Cl-4 PCB

	291.9194	M+2	12C12 H6 35Cl3 37Cl	Cl-4 PCB

	293.9165	M+4	12C12 H6 35Cl2 37Cl2	Cl-4 PCB

	301.9626	M+2	13C12 H6 35Cl3 37Cl	13C12 Cl-4 PCB

	303.9597	M+4	13C12 H6 35Cl2 37Cl2	13C12 Cl-4 PCB

	323.8834	M	12C12 H5 35Cl5	Cl-5 PCB

	325.8804	M+2	12C12 H5 35Cl4 37Cl	Cl-5 PCB

	327.8775	M+4	12C12 H5 35Cl3 37Cl2	Cl-5 PCB

	330.9792	lock	C7 F15	PFK