[Federal Register Volume 83, Number 5 (Monday, January 8, 2018)]
[Notices]
[Pages 800-801]
[FR Doc No: 2018-00091]

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DEPARTMENT OF ENERGY

Federal Energy Regulatory Commission

[Docket No. CP18-18-000]

Notice of Intent To Prepare an Environmental Assessment for the
Transcontinental Gas Pipe Line Company LLC Proposed Gateway Expansion
Project, and Request for Comments on Environmental Issues

    The staff of the Federal Energy Regulatory Commission (FERC or
Commission) will prepare an environmental assessment (EA) that will
discuss the environmental impacts of the Gateway Expansion Project
involving construction and operation of facilities Transcontinental Gas
Pipe Line Company (Transco) in Essex and Passaic Counties, New Jersey.
The Commission will use this EA in its decision-making process to
determine whether the project is in the public convenience and
necessity.
    This notice announces the opening of the scoping process the
Commission will use to gather input from the public and interested
agencies on the project. You can make a difference by providing us with
your specific comments or concerns about the project. Your comments
should focus on the potential environmental effects, reasonable
alternatives, and measures to avoid or lessen environmental impacts.
Your input will help the Commission staff determine what issues they
need to evaluate in the EA. To ensure that your comments are timely and
properly recorded, please send your comments so that the Commission
receives them in Washington, DC on or before February 2, 2018.
    If you sent comments on this project to the Commission before the
opening of this docket on November 16, 2017, you will need to file
those comments in Docket No. CP18-18-000 to ensure they are considered
as part of this proceeding.
    This notice is being sent to the Commission's current environmental
mailing list for this project. State and local government
representatives should notify their constituents of this proposed
project and encourage them to comment on their areas of concern.
    If you are a landowner receiving this notice, a pipeline company
representative may contact you about the acquisition of an easement to
construct, operate, and maintain the proposed facilities. The company
would seek to negotiate a mutually acceptable agreement. However, if
the Commission approves the project, that approval conveys with it the
right of eminent domain. Therefore, if easement negotiations fail to
produce an agreement, the pipeline company could initiate condemnation
proceedings where compensation would be determined in accordance with
state law.
    Transco provided landowners with a fact sheet prepared by the FERC
entitled An Interstate Natural Gas Facility On My Land? What Do I Need
To Know? This fact sheet addresses a number of typically asked
questions, including the use of eminent domain and how to participate
in the Commission's proceedings. It is also available for viewing on
the FERC website (www.ferc.gov).

Public Participation

    For your convenience, there are three methods you can use to submit
your comments to the Commission. The Commission encourages electronic
filing of comments and has expert staff available to assist you at
(202) 502-8258 or [email protected]. Please carefully follow
these instructions so that your comments are properly recorded.
    (1) You can file your comments electronically using the eComment
feature on the Commission's website (www.ferc.gov) under the link to
Documents and Filings. This is an easy method for submitting brief,
text-only comments on a project;
    (2) You can file your comments electronically by using the eFiling
feature on the Commission's website (www.ferc.gov) under the link to
Documents and Filings. With eFiling, you can provide comments in a
variety of formats by attaching them as a file with your submission.
New eFiling users must first create an account by clicking on
eRegister. If you are filing a comment on a particular project, please
select ``Comment on a Filing'' as the filing type; or
    (3) You can file a paper copy of your comments by mailing them to
the following address. Be sure to reference the project docket number
CP18-18-000 with your submission: Kimberly D. Bose, Secretary, Federal
Energy Regulatory Commission, 888 First Street NE, Room 1A, Washington,
DC 20426.

Summary of the Proposed Project

    Transco proposes to perform the following activities for
construction of the project.

Compressor Station (CS) 303 (Essex County, New Jersey)

     Expansion of the existing building to include one new
33,000 horsepower (hp) electric-motor driven compression unit and
ancillary equipment;
     Install gas cooling equipment;
     Extend existing security fencing to encompass new
equipment; and
     A new driveway to connect the new gas cooler location with
CS 303.

Roseland Meter and Regulator (M&R), Essex County, New Jersey

     One new 36-inch ML block valve with automation controls;
and
     Actuator modification on existing ML block valve.

Paterson M&R, Passaic County, New Jersey

     Remove existing 12-inch headers, meter skid, building, and
associated equipment;
     Install a meter skid with two 6-inch ultrasonic meters
with 12-inch inlet and outlet headers;
     Install a new M&R building;
     Install new remote terminal unit (RTU)/gas chromatograph
building;
     Install new flow computer, radio, and an antenna/pole; and
     Install a new condensate tank.
    The general location of the project facilities is shown in appendix
1.\1\
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    \1\ The appendices referenced in this notice will not appear in
the Federal Register. Copies of appendices were sent to all those
receiving this notice in the mail and are available at www.ferc.gov
using the link called eLibrary or from the Commission's Public
Reference Room, 888 First Street NE, Washington, DC 20426, or call
(202) 502-8371. For instructions on connecting to eLibrary, refer to
the last page of this notice.
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Land Requirements for Construction

    Construction of the proposed facilities would disturb 9.43 acres of
land, with 9.06 acres being utilized for the CS 303 site and the
remaining 0.37 acres utilized for the Paterson M&R site. All proposed
land affected is currently in use as Transco existing property. No new
land would need to be acquired for operational use.

The EA Process

    The National Environmental Policy Act (NEPA) requires the
Commission to take into account the environmental impacts that could
result from an action whenever it considers the issuance of a

Certificate of Public Convenience and Necessity. NEPA also requires us
\2\ to discover and address concerns the public may have about
proposals. This process is referred to as ``scoping.'' The main goal of
the scoping process is to focus the analysis in the EA on the important
environmental issues. By this notice, the Commission requests public
comments on the scope of the issues to address in the EA. We will
consider all filed comments during the preparation of the EA.
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    \2\ We, us, and our refer to the environmental staff of the
Commission's Office of Energy Projects.
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    In the EA we will discuss impacts that could occur as a result of
the construction and operation of the proposed project under these
general headings:
     Geology and soils;
     land use;
     water resources, fisheries, and wetlands;
     cultural resources;
     vegetation and wildlife;
     air quality and noise;
     endangered and threatened species;
     public safety; and
     cumulative impacts
    We will also evaluate reasonable alternatives to the proposed
project or portions of the project, and make recommendations on how to
lessen or avoid impacts on the various resource areas.
    The EA will present our independent analysis of the issues. The EA
will be available in the public record through eLibrary. We will
consider all comments on the EA before making our recommendations to
the Commission. To ensure we have the opportunity to consider and
address your comments, please carefully follow the instructions in the
Public Participation section, beginning on page 2.
    With this notice, we are asking agencies with jurisdiction by law
and/or special expertise with respect to the environmental issues of
this project to formally cooperate with us in the preparation of the
EA.\3\ Agencies that would like to request cooperating agency status
should follow the instructions for filing comments provided under the
Public Participation section of this notice. Currently, no agency has
expressed intention to participate as a cooperating agency in the
preparation of the EA.
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    \3\ The Council on Environmental Quality regulations addressing
cooperating agency responsibilities are at Title 40, Code of Federal
Regulations, Part 1501.6.
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Consultations Under Section 106 of the National Historic Preservation
Act

    In accordance with the Advisory Council on Historic Preservation's
implementing regulations for section 106 of the National Historic
Preservation Act, we are using this notice to initiate consultation
with the applicable State Historic Preservation Office (SHPO), and to
solicit their views and those of other government agencies, interested
Indian tribes, and the public on the project's potential effects on
historic properties.\4\ We will define the project-specific Area of
Potential Effects (APE) in consultation with the SHPO as the project
develops. On natural gas facility projects, the APE at a minimum
encompasses all areas subject to ground disturbance (examples include
construction right-of-way, contractor/pipe storage yards, compressor
stations, and access roads). Our EA for this project will document our
findings on the impacts on historic properties and summarize the status
of consultations under section 106.
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    \4\ The Advisory Council on Historic Preservation's regulations
are at Title 36, Code of Federal Regulations, Part 800. Those
regulations define historic properties as any prehistoric or
historic district, site, building, structure, or object included in
or eligible for inclusion in the National Register of Historic
Places.
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Environmental Mailing List

    The environmental mailing list includes federal, state, and local
government representatives and agencies; elected officials;
environmental and public interest groups; Native American Tribes; other
interested parties; and local libraries and newspapers. This list also
includes all affected landowners (as defined in the Commission's
regulations) who are potential right-of-way grantors, whose property
may be used temporarily for project purposes, or who own homes within
certain distances of aboveground facilities, and anyone who submits
comments on the project. We will update the environmental mailing list
as the analysis proceeds to ensure that we send the information related
to this environmental review to all individuals, organizations, and
government entities interested in and/or potentially affected by the
proposed project.
    If we publish and distribute the EA, copies will be sent to the
environmental mailing list for public review and comment. If you would
prefer to receive a paper copy of the document instead of the CD
version or would like to remove your name from the mailing list, please
return the attached Information Request (appendix 2).

Becoming an Intervenor

    In addition to involvement in the EA scoping process, you may want
to become an ``intervenor'' which is an official party to the
Commission's proceeding. Intervenors play a more formal role in the
process and are able to file briefs, appear at hearings, and be heard
by the courts if they choose to appeal the Commission's final ruling.
An intervenor formally participates in the proceeding by filing a
request to intervene. Instructions for becoming an intervenor are in
the ``Document-less Intervention Guide'' under the ``e-filing'' link on
the Commission's website. Motions to intervene are more fully described
at http://www.ferc.gov/resources/guides/how-to/intervene.asp.

Additional Information

    Additional information about the project is available from the
Commission's Office of External Affairs, at (866) 208-FERC, or on the
FERC website at www.ferc.gov using the ``eLibrary'' link. Click on the
eLibrary link, click on ``General Search'' and enter the docket number,
excluding the last three digits in the Docket Number field (i.e., CP18-
18). Be sure you have selected an appropriate date range. For
assistance, please contact FERC Online Support at
[email protected] or toll free at<PHONE_NUMBER>, or for TTY,
contact<PHONE_NUMBER>. The eLibrary link also provides access to the
texts of formal documents issued by the Commission, such as orders,
notices, and rulemakings.
    In addition, the Commission offers a free service called
eSubscription which allows you to keep track of all formal issuances
and submittals in specific dockets. This can reduce the amount of time
you spend researching proceedings by automatically providing you with
notification of these filings, document summaries, and direct links to
the documents. Go to www.ferc.gov/docs-filing/esubscription.asp.

    Dated: January 2, 2018.
Kimberly D. Bose,
 Secretary.
[FR Doc. 2018-00091 Filed 1-5-18; 8:45 am]
 BILLING CODE 6717-01-P


[Federal Register Volume 82, Number 213 (Monday, November 6, 2017)]
[Notices]
[Pages 51471-51472]
[FR Doc No: 2017-24077]

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DEPARTMENT OF TRANSPORTATION

Public Availability of the Department of Transportation FY 2016
Service Contract Inventory

AGENCY: Department of Transportation.

ACTION: Notice of Public Availability of FY 2016 Service Contract
Inventory.

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    In accordance with Section 743 of Division C of the Consolidated
Appropriations Act of 2010, Public Law 111-117, Department of
Transportation is publishing this notice to advise the public of the
availability of the FY 2016 Government-wide Service Contact Inventory
data, the analysis of the FY 2015 Service Contract Inventory data and
the plan for analyzing the FY 2016 data. This inventory provides
information on service contract actions over $25,000 awarded in FY
2016. The information is organized by function to show how contracted
resources are distributed throughout the agency.
    The inventory has been developed in accordance with guidance issued
on November 5, 2010 by the Office of Management and Budget's Office of
Federal Procurement Policy (OFPP). OFPP's guidance is available at
http://www.whitehouse.gov/sites/default...052010.pdf. Department of
Transportation has posted its analysis of the FY 2015 Service Contact
Inventory

data, the plan for analyzing the FY 2016 data, and the link to the FY
2016 Government-wide inventory on the Department of Transportation's
homepage at the following link: https://www.transportation.gov/assistant-secretary-administration/procurement/service-contract-inventory. Questions regarding the Service Contract Inventory should be
directed to Diane Morrison in the Office of the Senior Procurement
Executive at<PHONE_NUMBER> or [email protected].

    Dated: October 3, 2017.
Gregory Cate,
Deputy Director, Office of Senior Procurement Executive.
[FR Doc. 2017-24077 Filed 11-3-17; 8:45 am]
 BILLING CODE 4910-9X-P


NOTE: The filtration procedures specified in Sections <IP_ADDRESS>  <IP_ADDRESS>.3 are specific to laboratory filtration of a bulk sample. These procedures may require modification if samples will be filtered in the field.

12.3.1 Filtration <IP_ADDRESS> Flow rate adjustment <IP_ADDRESS>.1 Connect the sampling system, minus the filter housing, to a carboy filled with reagent water. <IP_ADDRESS>.2 Place the peristaltic pump upstream of the filter housing. <IP_ADDRESS>.3 Turn on the pump and adjust the flow rate to 1 to 4 L per minute.

NOTE: A head pressure of 0.5 bar ( 7.5 psi) is required to create flow through the filter, and the recommended pressure of 5 bar ( 75 psi) should produce the flow rate of 3 to 4 L per minute. The maximum operating pressure of 8 bar ( 120 psi) should not be exceeded.

<IP_ADDRESS>.4 Allow 2 to 10 L of reagent water to flush the system. Adjust the pump speed as necessary during this period. Turn off the pump when the flow rate has been adjusted. <IP_ADDRESS> Place filter module into the filter housing and secure lid, hand tighten housings, apply gentle pressure to create the seal between the module and the ` O' rings in the base and the lid of the housing. Excessive tightening is not necessary, and may shorten the life of the ` O' rings. Tools may be used to tighten housing to the alignment marks ( refer to manufacturer's instructions). ` O' rings should be lightly greased before use ( refer to manufacturer's instructions). <IP_ADDRESS> Install the filter housing in the line, securing the inlet and outlet ends with the appropriate clamps/ fittings. Verify that the filter housing is installed so that the end closest to the screw top cap is the inlet and the opposite end is the outlet. <IP_ADDRESS> Record the sample number, sample turbidity ( if not provided with the field sample), and the name of the analyst filtering the sample on a bench sheet. <IP_ADDRESS> Filtration <IP_ADDRESS>.1 Connect the sampling system to the field carboy of sample water, or transfer the sample water to the laboratory carboy used in Section <IP_ADDRESS>.1. If the sample will be filtered from a field carboy, a spigot can be used with the carboy to facilitate sample filtration.

NOTE: If the bulk field sample is transferred to a laboratory carboy, the laboratory carboy must be cleaned and disinfected before it is used with another field sample.

<IP_ADDRESS>.2 Place the drain end of the sampling system tubing into an empty graduated container with a capacity greater than or equal to the volume to be filtered. This container will be used to determine the sample volume filtered. Method 1622  Cryptosporidium June 2003 38 Alternately, connect a flow meter downstream of the filter, and record the initial meter reading. <IP_ADDRESS>.3 Allow the carboy discharge tube and filter housing to fill with sample water. Turn on the pump to start water flowing through the filter. Verify that the flow rate is between 1 and 4 L per min. <IP_ADDRESS>.4 After all of the sample has passed through the filter, turn off the pump. Allow the pressure to decrease until flow stops. <IP_ADDRESS> Disassembly <IP_ADDRESS>.1 Disconnect the inlet end of the filter housing assembly while maintaining the level of the inlet fitting above the level of the outlet fitting to prevent backwashing and the loss of oocysts from the filter. Restart the pump and allow as much water to drain as possible. Turn off the pump. <IP_ADDRESS>.2 Based on the water level in the graduated container or the meter reading, record the volume filtered on a bench sheet to the nearest quarter liter. <IP_ADDRESS>.3 Loosen the outlet fitting, the filter housing should be sealed with rubber plugs.

NOTE: Filters should be prevented from drying out, as this can impair their ability to expand when decompressed.

12.3.2 Elution <IP_ADDRESS> The filter is eluted to wash the oocysts from the filter. This can be accomplished using the Filta Max   wash station, which moves a plunger up and down a tube containing the filter and eluting solution ( Section <IP_ADDRESS>), or a stomacher, which uses paddles to agitate the stomacher bag containing the foam filter in the eluting solution ( Section <IP_ADDRESS>). If the Filta MaxTM automatic wash station is used please see the manufacturer's operator's guide for instructions on its use. <IP_ADDRESS> Filta Max   wash station elution procedure <IP_ADDRESS>.1 First wash ( a) Detach the removable plunger head using the tool provided, and remove the splash guard. ( b) Place the filter membrane flat in the concentrator base with the rough side up. Locate the concentrator base in the jaws of the wash station and screw on the concentrator tube ( the longer of the two tubes), creating a tight seal at the membrane. Take the assembled concentrator out of the jaws and place on the bench. ( c) Replace the splash guard and temporarily secure it at least 15 cm above the end of the rack. Secure the plunger head with the tool provided ensuring that the lever is fully locked down. ( d) Remove the filter module from the filter housing or transportation container. Pour excess liquid into the assembled concentrator, then rinse the housing or Method 1622  Cryptosporidium June 2003 39 container with PBST and add the rinse to the concentrator tube. Screw the filter module onto the base of the plunger. Locate the elution tube base in the jaws of the wash station and screw the elution tube ( the shorter of the two tubes) firmly in place. ( e) Pull the plunger down until the filter module sits at the bottom of the elution tube; the locking pin ( at the top left of the wash station) should " click" to lock the plunger in position. ( f) Remove the filter module bolt by turning the adapted allen key ( provided) in a clockwise direction ( as seen from above). Attach the steel tube to the elution tube base. ( g) Add 600 mL of PBST to the assembled concentrator. If more than 50 mL of liquid has been recovered from the shipped filter module, reduce the volume of PBST accordingly. Screw the concentrator tube onto the base beneath the elution tube. Release the locking pin.

NOTE: Gentle pressure on the lever, coupled with a pulling action on the locking pin should enable the pin to be easily released.

( h) Wash the foam disks by moving the plunger up and down 20 times. Gentle movements of the plunger are recommended to avoid generating excess foam.

NOTE: The plunger has an upper movement limit during the wash process to prevent it popping out of the top of the chamber.

( i) Detach the concentrator and hold it such that the stainless steel tube is just above the level of the liquid. Purge the remaining liquid from the elution tube by moving the plunger up and down 5 times, then lock the plunger in place. To prevent drips, place the plug provided in the end of the steel tube. ( j) Prior to the second wash the eluate from the first wash can be concentrated using the Filta Max   apparatus according to Section <IP_ADDRESS>.1 or the eluate can be decanted into a 2 L pooling beaker and set aside. <IP_ADDRESS>.2 Second wash ( a) Add an additional 600 mL of PBST to the concentrator module, remove the plug from the end of the steel tube and screw the concentrator tube back onto the elution module base. Release the locking pin. ( b) Wash the foam disks by moving the plunger up and down 10 times. Gentle movements of the plunger are recommended to avoid generating excess foam. Method 1622  Cryptosporidium June 2003 40 ( c) The eluate can be concentrated using the Filta Max   apparatus according to Section <IP_ADDRESS>.2 or the eluate can be decanted into the 2 L pooling beaker containing the eluate from the first wash and concentrated using centrifugation, as described in Section <IP_ADDRESS>. <IP_ADDRESS> Stomacher elution procedure <IP_ADDRESS>.1 First wash ( a) Place the filter module in the stomacher bag then use the allen key to remove the bolt from the filter module, allowing the rings to expand. Remove the end caps from the stomacher bag and rinse with PBST into the stomacher bag. ( b) Add 600 mL of PBST to stomacher bag containing the filter pads. Place bag in stomacher and wash for 5 minutes on a normal setting. ( c) Remove the bag from the stomacher and decant the eluate into a 2 L pooling beaker. <IP_ADDRESS>.2 Second wash ( a) Add a second 600 mL aliquot of PBST to the stomacher bag. Place bag in stomacher and wash for 5 minutes on a normal setting. Remove the bag from the stomacher and decant the eluate from the stomacher bag into the 2 L pooling beaker. Wring the stomacher bag by hand to remove eluate from the foam filter and add to the pooling beaker. Remove the foam filter from the bag and using a squirt bottle, rinse the stomacher bag with reagent water and add the rinse to the pooling beaker. ( b) Proceed to concentration ( Section 12.3.3). 12.3.3 Concentration <IP_ADDRESS> The eluate can be concentrated using the Filta Max   concentrator apparatus, which pulls most of the eluate through a membrane filter leaving the oocysts concentrated in a small volume of the remaining eluting solution ( Section 12.3.. 2), or by directly centrifuging all of the eluting solution used to wash the filter ( Section <IP_ADDRESS>). <IP_ADDRESS> The Filta Max   concentrator procedure <IP_ADDRESS>.1 Concentration of first wash ( a) If the stomacher was used to elute the sample ( Section <IP_ADDRESS>), transfer 600 mL of eluate from the pooling beaker to the concentrator tube. Otherwise proceed to Step ( b). ( b) Stand the concentrator tube on a magnetic stirring plate and attach the lid ( with magnetic stirrer bar). Connect the waste bottle trap and hand or electric vacuum pump to the valve on the concentrator base. Begin stirring and open the tap. Increase the vacuum using the hand pump. Method 1622  Cryptosporidium June 2003 41 NOTE: The force of the vacuum should not exceed 30 cmHg.

( c) Allow the liquid to drain until it is approximately level with the middle of the stirrer bar then close the valve. Remove the magnetic stirrer, and rinse it with PBST or distilled water to recover all oocysts. Decant the concentrate into a 50 mL tube, then rinse the sides of the concentration tube and add the rinsate to the 50 mL tube. <IP_ADDRESS>.2 Concentration of second wash ( a) If the stomacher was used to elute the sample ( Section <IP_ADDRESS>), transfer the remaining 600 mL of eluate from the pooling beaker to the concentrator tube. Otherwise proceed to Step ( b). ( b) Add the concentrate, in the 50 mL tube, retained from the first concentration ( Section <IP_ADDRESS>.1 ( c)) to the 600 mL of eluate from the second wash, then repeat concentration steps from Sections <IP_ADDRESS>.1 ( b) and <IP_ADDRESS>.1 ( c). The final sample can be poured into the same 50 mL tube used to retain the first concentrate. Rinse the sides of the concentrator tube with PBST and add the rinse to the 50 mL tube. ( c) Remove the magnetic stirrer. Insert the empty concentrator module into the jaws of the wash station and twist off the concentrator tube. ( d) Transfer the membrane from the concentrator base to the bag provided using membrane forceps. <IP_ADDRESS>.3 Membrane elution. The membrane can be washed manually or using a stomacher: ° Manual wash. Add 5 mL of PBST to the bag containing the membrane. Rub the surface of the membrane through the bag until the membrane appears clean. Using a pipette, transfer the eluate to a 50 mL tube. Repeat the membrane wash with another 5 mL of PBST and transfer the eluate to the 50 mL tube. ( Optional: Perform a third wash using another 5 mL of PBST, by hand kneading an additional minute or placing the bag on a flat headed vortexer and vortexing for one minute. Transfer the eluate to the 50 mL tube.)

NOTE: Mark the bag with an " X" to note which side of the membrane has the oocysts to encourage the hand kneading to focus on the appropriate side of the membrane.

° Stomacher wash. Add 5 mL of PBST to the bag containing the membrane. Place the bag containing the membrane into a small stomacher and stomach for 3 minutes. Using a pipette transfer the eluate to a 50 mL tube. Repeat the wash two times using the stomacher and 5 mL aliquots of PBST. ( Optional: Method 1622  Cryptosporidium June 2003 42 Perform a fourth wash using another 5 mL of PBST, by hand kneading an additional minute or placing the bag on a flat headed vortexer and vortexing for one minute. Transfer the eluate to the 50 mL tube.) <IP_ADDRESS>.4 If the membrane filter clogs before concentration is complete, there are two possible options for completion of concentration. One option is replacing the membrane as often as necessary. Filter membranes may be placed smooth side up during the second concentration step. Another option is concentrating the remaining eluate using centrifugation. Both options are provided below. ° Using multiple membranes. Disassemble the concentrator tube and pour any remaining eluate back into the pooling beaker. Remove the membrane using membrane forceps, placing it in the bag provided. Place a new membrane in the concentrator tube smooth side up, reassemble, return the eluate to the concentrator tube, rinse the pooling beaker and add rinse to the eluate, and continue the concentration. Replace the membrane as often as necessary. ° Centrifuging remaining volume. Decant the remaining eluate into a 2 L pooling beaker. Rinse the sides of the concentrator tube and add to the pooling beaker. Remove the filter membrane and place it in the bag provided. Wash the membrane as described in Section <IP_ADDRESS>.3, then concentrate the sample as described in Section <IP_ADDRESS>.1. <IP_ADDRESS> If the Filta Max   concentrator is not used for sample concentration, or if the membrane filter clogs before sample concentration is complete, then the procedures described in Section <IP_ADDRESS>.1 should be used to concentrate the sample. If less than 50 mL of concentrate has been generated, the sample can be further concentrated, as described in Section <IP_ADDRESS>.2, to reduce the volume of sample to be processed through IMS.

NOTE: The volume must not be reduced to less than 5 mL above the packed pellet. The maximum amount of pellet that should be processed through IMS is 0.5 mL. If the packed pellet is greater than 0.5 mL then the pellet may be subsampled as described in Section 13.2.4.

<IP_ADDRESS>.1 Centrifugation of greater than 50 mL of eluate ( a) Decant the eluate from the 2 L pooling beaker into 250 mL conical centrifuge tubes. Make sure that the centrifuge tubes are balanced.

( b) Centrifuge the 250 mL centrifuge tubes containing the eluate at 1500 × G for 15 minutes. Allow the centrifuge to coast to a stop.

( c) Using a Pasteur pipette, carefully aspirate off the supernatant to 5 mL above the pellet. If the sample is reagent water ( e. g. initial or ongoing precision and Method 1622  Cryptosporidium June 2003 43 recovery sample) extra care must be taken to avoid aspirating oocysts during this step.

( d) Vortex each 250 mL tube vigorously until pellet is completely resuspended. Swirl the centrifuge tube gently to reduce any foaming after vortexing. Combine the contents of each 250 mL centrifuge tube into a 50 mL centrifuge tube. Rinse each of the 250 mL centrifuge tubes with PBST and add the rinse to the 50 mL tube.

( e) Proceed to Section <IP_ADDRESS>.2. <IP_ADDRESS>.2 Centrifugation of less than 50 mL of eluate ( a) Centrifuge the 50 mL centrifuge tube containing the combined concentrate at 1500 x G for 15 minutes. Allow the centrifuge to coast to a stop. Record the initial pellet volume ( volume of solids) and the date and time that concentration was completed on a bench sheet. ( b) Proceed to Section 13.0 for concentration and separation ( purification). 12.3.4 Maintenance and cleaning <IP_ADDRESS> Maintenance of O rings <IP_ADDRESS>.1 Check all rubber O rings for wear or deterioration prior to each use and replace as necessary. <IP_ADDRESS>.2 Lubricate the plunger head O ring inside and out with silicon before each use. <IP_ADDRESS>.3 Lubricate all other O rings ( concentrator tube set, filter housing) regularly in order to preserve their condition. <IP_ADDRESS> Cleaning <IP_ADDRESS>.1 All components of the Filta MaxTM system can be cleaned using warm water and laboratory detergent. After washing, rinse all components with oocyst free reagent water and dry them. All O rings should be relubricated Alternatively a mild ( 40 ° C) dishwasher cycle without bleach or rinse aid can be used. <IP_ADDRESS>.2 To wash the detachable plunger head slide the locking pin out and wash the plunger head and locking pin in warm water and laboratory detergent. Rinse the plunger head and locking pin with oocyst free reagent water and dry. Lightly lubricate the locking pin and re assemble the plunger head.

13.0 Sample Concentration and Separation ( Purification) 13.1 During concentration and separation, the filter eluate is concentrated through centrifugation, and the oocysts in the sample are separated from other particulates through immunomagnetic separation ( IMS). Alternate procedures and products may be used if the laboratory first demonstrates equivalent or superior performance as per Section 9.1.2. Method 1622  Cryptosporidium June 2003 44 13.2 Adjustment of pellet volume 13.2.1 Centrifuge the 250 mL centrifuge tube containing the capsule filter eluate at 1500 × G for 15 minutes. Allow the centrifuge to coast to a stop   do not use the brake. Record the pellet volume ( volume of solids) on the bench sheet.

NOTE: Recoveries may be improved if centrifugation force is increased to 2000 × G. However, do not use this higher force if the sample contains sand or other gritty material that may degrade the condition of any oocysts in the sample.

13.2.2 Using a Pasteur pipette, carefully aspirate the supernatant to 5 mL above the pellet. Extra care must be taken to avoid aspirating oocysts during this step, particularly if the sample is reagent water ( e. g. initial or ongoing precision and recovery sample). 13.2.3 If the packed pellet volume is # 0.5 mL, vortex the tube vigorously until pellet is completely resuspended. Swirl the centrifuge tube gently to reduce any foaming after vortexing. Record the resuspended pellet volume on the bench sheet. Proceed to Section 13.3.

NOTE: Extra care must be taken with samples containing sand or other gritty material when vortexing to ensure that the condition of any oocysts in the sample is not compromised.

13.2.4 If the packed pellet volume is > 0.5 mL, the concentrate needs to must be separated into multiple subsamples ( a subsample is equivalent to no greater than 0.5 mL of packed pellet material, the recommended maximum amount of particulate material to process through the subsequent purification and examination steps in the method). Use the following formula to determine the total volume required in the centrifuge tube before separating the concentrate into two or more subsamples:

total volume ( mL) required = pellet volume x 5 mL 0.5 mL ( For example, if the packed pellet volume is 1.2 mL, the total volume required is 12 mL.) Add reagent water to the centrifuge tube to bring the total volume to the level calculated above. Vortex the tube vigorously for 10 to 15 seconds to completely resuspend the pellet. Record the resuspended pellet volume on the bench sheet.

NOTE: Extra care must be taken with samples containing sand or other gritty material when vortexing to ensure that the condition of any oocysts in the sample is not compromised.

<IP_ADDRESS> Analysis of entire sample. If analysis of the entire sample is required, determine the number of subsamples to be processed independently through the remainder of the method: <IP_ADDRESS>.1 Calculate number of subsamples: Divide the total volume in the centrifuge tube by 5 mL and round up to the nearest integer ( for example, if the resuspended volume in Section 13.2.4 is 12 mL, then the number of subsamples would be 12 mL / 5 mL = 2.4, rounded = 3 subsamples). <IP_ADDRESS>.2 Determine volume of resuspended concentrate per subsample. Divide the total volume in the centrifuge tube by the calculated number of subsamples ( for example, if the resuspended volume in Section 13.2.4 is Method 1622  Cryptosporidium June 2003 45 12 mL, then the volume to use for each subsample = 12 mL / 3 subsamples = 4 mL). <IP_ADDRESS>.3 Process subsamples through IMS. Vortex the tube vigorously for 10 to 15 seconds to completely resuspend the pellet. Record the resuspended pellet volume on the bench sheet. Proceed immediately to Section 13.3, and transfer aliquots of the resuspended concentrate equivalent to the volume in the previous step to multiple, flat sided sample tubes in Section <IP_ADDRESS>. Process the sample as multiple, independent subsamples from Section 13.3 onward, including the preparation and examination of separate slides for each aliquot. Record the volume of resuspended concentrate transferred to IMS on the bench sheet ( this will be equal to the volume recorded in Section 13.2.4). Also record the number of subsamples processed independently through the method on the bench sheet. <IP_ADDRESS> Analysis of partial sample. If not all of the concentrate will be examined, vortex the tube vigorously for 10 to 15 seconds to completely resuspend the pellet. Record the resuspended pellet volume on the bench sheet. Proceed immediately to Section 13.3, and transfer one or more 5 mL aliquots of the resuspended concentrate to one or more flat sided sample tubes in Section <IP_ADDRESS>. Record the volume of resuspended concentrate transferred to IMS on the bench sheet. To determine the volume analyzed, calculate the percent of the concentrate examined using the following formula:

percent examined = total volume of resuspended concentrate transferred to IMS x 100% total volume of resuspended concentrate in Section 13.2.4 Then multiply the volume filtered ( Section <IP_ADDRESS>) by this percentage to determine the volume analyzed. 13.3 IMS procedure ( adapted from Reference 20.11)

NOTE: The IMS procedure should be performed on a bench top with all materials at room temperature, ranging from 15 ° C to 25 ° C.

13.3.1 Preparation and addition of reagents <IP_ADDRESS> Prepare a 1X dilution of SL buffer A from the 10X SL buffer A ( clear, colorless solution) supplied. Use reagent water ( demineralized; Section 7.3) as the diluent. For every 1 mL of 1X SL buffer A required, take 100 µ L of 10X SL buffer A and make up to 1 mL with the diluent water. A volume of 1.5 mL of 1X SL buffer A will be required per sample or subsample on which the Dynal IMS procedure is performed. <IP_ADDRESS> For each sample or subsample ( Section 13.2) to be processed through IMS, add 1 mL of the 10X SL buffer A ( supplied   not the diluted 1X SL buffer A) to a flat sided tube ( Section 6.5.4). <IP_ADDRESS> For each subsample, add 1 mL of the 10X SL buffer B ( supplied   magenta solution) to the flat sided tube containing the 10X SL buffer A. Method 1622  Cryptosporidium June 2003 46 13.3.2 Oocyst capture <IP_ADDRESS> Use a graduated, 10 mL pipette that has been pre rinsed with elution buffer to transfer the water sample concentrate from Section 13.2 to the flat sided tube( s) containing the SL buffers. If all of the concentrate is used, rinse the centrifuge tube twice with reagent water and add the rinsate to the flat sided tube containing the concentrate ( or to the tube containing the first subsample, if multiple subsamples will be processed). Each of the two rinses should be half the volume needed to bring the volume in the flat sided sample tube to 10 mL ( including the buffers added in Sections <IP_ADDRESS> and <IP_ADDRESS>). ( For example, if the tube contained 1 mL of SL buffer A and 1 mL of SL buffer B, and 5 mL of sample was transferred after resuspension of the pellet, for a total of 7 mL, the centrifuge tube would be rinsed twice with 1.5 mL of reagent water to bring the total volume in the flat sided tube to 10 mL.) Visually inspect the centrifuge tube after completing the transfer to ensure that no concentrate remains. If multiple subsamples will be processed, bring the volume in the remaining flat sided tubes to 10 mL with reagent water. Label the flat sided tube( s) with the sample number ( and subsample letters). <IP_ADDRESS> Vortex the Dynabeads ® anti Cyptosporidium vial from the IMS kit for approximately 10 seconds to suspend the beads. Ensure that the beads are fully resuspended by inverting the sample tube and making sure that there is no residual pellet at the bottom. <IP_ADDRESS> Add 100 µ L of the resuspended Dynabeads ® anti Cryptosporidium ( Section <IP_ADDRESS>) to the sample tube( s) containing the water sample concentrate and SL buffer. <IP_ADDRESS> Affix the sample tube( s) to a rotating mixer and rotate at approximately 18 rpm for 1 hour at room temperature. <IP_ADDRESS> After rotating for 1 hour, remove each sample tube from the mixer and place the tube in the magnetic particle concentrator ( MPC 1) with flat side of the tube toward the magnet. <IP_ADDRESS> Without removing the sample tube from the MPC 1, place the magnet side of the MPC 1 downwards, so the tube is horizontal and the flat side of the tube is facing down. <IP_ADDRESS> Gently rock the sample tube by hand end to end through approximately 90 ° , tilting the cap end and base end of the tube up and down in turn. Continue the tilting action for 2 minutes with approximately one tilt per second. <IP_ADDRESS> Ensure that the tilting action is continued throughout this period to prevent binding of low mass, magnetic or magnetizable material. If the sample in the MPC 1 is allowed to stand motionless for more than 10 seconds, remove the flat sided tube from the MPC 1, shake the tube to resuspend all material, replace the sample tube in the MPC 1 and repeat Section <IP_ADDRESS> before continuing to Section <IP_ADDRESS>. <IP_ADDRESS> Return the MPC 1 to the upright position, sample tube vertical, with cap at top. Immediately remove the cap and, keeping the flat side of the tube on top, pour off all of the supernatant from the tube held in the MPC 1 into a suitable container. Do not shake the tube and do not remove the tube from MPC 1 during this step. Allow more supernatant to settle; aspirate additional supernatant with pipette. Method 1622  Cryptosporidium June 2003 47 <IP_ADDRESS> Remove the sample tube from the MPC 1 and resuspend the sample in 1 mL 0.5 mL 1X SL buffer A ( prepared from 10X SL buffer A stock   supplied). Mix very gently to resuspend all material in the tube. Do not vortex. <IP_ADDRESS> Quantitatively transfer ( transfer followed by two rinses) all the liquid from the sample tube to a labeled, 1.5 mL microcentrifuge tube. Use 1 mL 0.5 mL of 1X SL buffer A to perform the first rinse and 0.5 mL of reagent water for the second rinse. Liberally rinse down the sides of the Leighton tube before transferring and transfer the rinsate to the microcentrifuge tube. Allow the flat sided sample tube to sit for a minimum of 1 minute after transfer of the second rinse volume, then use a pipette to collect any residual volume that drips down to the bottom of the tube to ensure that as much sample volume is recovered as possible. Ensure that all of the liquid and beads are transferred. <IP_ADDRESS> Place the microcentrifuge tube into the second magnetic particle concentrator ( MPC M), with its magnetic strip in place. <IP_ADDRESS> Without removing the microcentrifuge tube from MPC M, gently rock/ roll the tube through 180 ° by hand. Continue for approximately 1 minute with approximately one 180 ° roll/ rock per second. At the end of this step, the beads should produce a distinct brown dot at the back of the tube. <IP_ADDRESS> Immediately aspirate the supernatant from the tube and cap held in the MPC M. If more than one sample is being processed, conduct three 90 ° rock/ roll actions before removing the supernatant from each tube. Take care not to disturb the material attached to the wall of the tube adjacent to the magnet. Do not shake the tube. Do not remove the tube from MPC M while conducting these steps. 13.3.3 Dissociation of beads/ oocyst/ cyst complex NOTE: Two acid dissociations are required.

<IP_ADDRESS> Remove the magnetic strip from the MPC M. <IP_ADDRESS> Add 50 µ L of 0.1 N HCl, then vortex at the highest setting for approximately 50 seconds.

NOTE: The laboratory should must use 0.1 N standards purchased directly from a vendor, rather than adjusting the normality in house.

<IP_ADDRESS> Place the tube in the MPC M without the magnetic strip in place and allow to stand in a vertical position for at least 10 minutes at room temperature. <IP_ADDRESS> Vortex vigorously for approximately 30 seconds. <IP_ADDRESS> Ensure that all of the sample is at the base of the tube. Place the microcentrifuge tube in the MPC M. <IP_ADDRESS> Replace magnetic strip in MPC M and allow the tube to stand undisturbed for a minimum of 10 seconds. <IP_ADDRESS> Prepare a well slide for sample screening and label the slide. <IP_ADDRESS> Add 5 µ L of 1.0 N NaOH to the sample wells of two well slides ( add 10 µ L to the sample well of one well slide if the volume from the two required dissociations will be added to the same slide). Method 1622  Cryptosporidium June 2003 48 NOTE: The laboratory should must use 1.0 N standards purchased directly from a vendor rather than adjusting the normality in house.

<IP_ADDRESS> Without removing the microcentrifuge tube from the MPC M, transfer all of the sample from the microcentrifuge tube in the MPC M to the sample well with the NaOH. Do not disturb the beads at the back wall of the tube. Ensure that all of the fluid is transferred. <IP_ADDRESS> Do not discard the beads or microcentrifuge tube after transferring the volume from the first acid dissociation to the well slide. Perform the steps in Sections <IP_ADDRESS> through <IP_ADDRESS> a second time. The volume from the second dissociation can be added to the slide containing the volume from the first dissociation, or can be applied to a second slide.

NOTE: If one slide is used, exert extra care when using The wells on Dynal Spot On slides are likely to be too small to accommodate the volumes from both dissociations.

<IP_ADDRESS> Record the date and time the purified sample was applied to the slide( s). <IP_ADDRESS> Air dry the sample on the well slide( s). Because temperature and humidity varyies from laboratory to laboratory, no minimum time is specified. However, the laboratory must take care to ensure that the sample has dried completely before staining to prevent losses during the rinse steps. A slide warmer set at 35 ° C to 42 ° C also can be used. 13.3.4 Tips for minimizing carry over of debris onto microscope slides after IMS ° Make sure the resuspended pellet is fully homogenized before placing the tube in the MPC 1 or MPC M to avoid trapping " clumps" or a dirty layer between the beads and the side of the tube.

° When using the MPC 1 magnet, make sure that the tube is snugged flat against the magnet. Push the tube flat if necessary. Sometimes the magnet is not flush with the outside of the holder and, therefore, the attraction between the beads and the magnet is not as strong as it should be. However, it can be difficult to determine this if you do not have more than one MPC 1 to make comparisons.

° After the supernatant has been poured off at Section <IP_ADDRESS>, leave the tube in the MPC 1 and allow time for any supernatant remaining in the tube to settle down to the bottom. Then aspirate the settled supernatant and associated particles from the bottom of the tube. The same can be done at Section <IP_ADDRESS> with the microcentrifuge tube.

° An additional rinse can also be performed at Section <IP_ADDRESS>. After the supernatant has been poured off and any settled material is aspirated off the bottom, leave the tube in the MPC 1 and add an additional 10 mL of reagent water or PBS to the tube and repeat Sections <IP_ADDRESS> and <IP_ADDRESS>. Although labs have reported successfully using this technique to reduce carryover, because the attraction between the MPC 1 and the beads is not as great as the attraction between the MPC M and the beads, the chances would be greater for loss of oocysts doing the rinse at this step instead of at Section <IP_ADDRESS>.

° After the supernatant has been aspirated from the tube at Section <IP_ADDRESS>, add 0.1 mL of PBS, remove the tube from the MPC M, and resuspend. Repeat Sections <IP_ADDRESS> and <IP_ADDRESS>.

° Use a slide with the largest diameter well available to spread out the sample as much as possible. Method 1622  Cryptosporidium June 2003 49 14.0 Sample Staining NOTE: The sample must be stained within 72 hours of application of the purified sample to the slide.

14.1 Prepare positive and negative controls. 14.1.1 For the positive control, pipette 10 µ L of positive antigen or 200 to 400 intact oocysts to the center of a well. 14.1.2 For the negative control, pipette 50 µ L of 150 mM PBS ( Section 7.6.4) into the center of a well and spread it over the well area with a pipette tip. 14.1.3 Air dry the control slides ( see Section <IP_ADDRESS> for guidance).

NOTE: If the laboratory has a large batch of slides that will be examined over several days, and is concerned that a single positive control may fade, due to multiple examinations, the laboratory should prepare multiple control slides with the batch of field slides and alternate between the positive controls when performing the positive control check.

14.2 Apply 50 µ L of absolute methanol to each well containing the dried sample and allow to air dry for 3 to 5 minutes. 14.2 Follow manufacturer's instructions in applying stain to slides. 14.3 Place the slides in a humid chamber in the dark and incubate at room temperature for approximately 30 minutes. The humid chamber consists of a tightly sealed plastic container containing damp paper towels on top of which the slides are placed. 14.4 Remove slides from humid chamber and allow condensation to evaporate, if present. 14.5 Apply one drop of wash buffer ( prepared according to the manufacturer's instructions [ Section 7.6]) to each well. Tilt each slide on a clean paper towel, long edge down. Gently aspirate the excess detection reagent from below the well using a clean Pasteur pipette or absorb with paper towel or other absorbent material placed at edge of slide. Avoid disturbing the sample.

NOTE: If using the Merifluor stain ( Section 7.6.1), do not allow slides to dry completely.

14.6 Apply 50 µ L of 4', 6 diamidino 2 phenylindole ( DAPI) staining solution ( Section 7.7.2) to each well. Allow to stand at room temperature for a minimum of 1 minute. ( The solution concentration may be increased up to 1 µ g/ mL if fading/ diffusion of DAPI staining is encountered, but the staining solution must be tested first on expendable environmental samples to confirm that staining intensity is appropriate.) 14.7 Apply one drop of wash buffer ( prepared according to the manufacturer's instructions [ Section 7.6]) to each well. Tilt each slide on a clean paper towel, long edge down. Gently aspirate the excess DAPI staining solution from below the well using a clean Pasteur pipette or absorb with paper towel or other absorbent material placed at edge of slide. Avoid disturbing the sample.

NOTE: If using the Merifluor stain ( Section 7.6.1), do not allow slides to dry completely.

14.8 Add mounting medium ( Section 7.8) to each well. 14.9 Apply a cover slip. Use a tissue to remove excess mounting fluid from the edges of the coverslip. Seal the edges of the coverslip onto the slide using clear nail polish. 14.10 Record the date and time that staining was completed on the bench sheet. If slides will not be read immediately, store in a humid chamber in the dark at 0 ° C to < 8 ° C ( but not frozen) until ready for examination. Method 1622  Cryptosporidium June 2003 50 15.0 Examination NOTE: Although immunofluorescence assay ( FA) and 4', 6 diamidino 2 phenylindole ( DAPI) and differential interference contrast ( DIC) microscopy examination and confirmation should be performed immediately after staining is complete, laboratories have up to 7 days from completion of sample staining to complete the examination and confirmation of samples. However, if fading/ diffusion of FITC or DAPI staining is noticed, the laboratory must reduce this holding time. In addition the laboratory may adjust the concentration of the DAPI staining solution ( Sections 7.7.2) so that fading/ diffusion does not occur.

15.1 Scanning technique: Scan each well in a systematic fashion. An up and down or a side to side scanning pattern may be used ( Figure 4). 15.2 Examination using immunofluorescence assay ( FA), 4', 6 diamidino 2 phenylindole ( DAPI) staining characteristics, and differential interference contrast ( DIC) microscopy. The minimum magnification requirements for each type of examination are noted below.

NOTE: All shape characterization and size measurements must be determined using 1000X magnification and reported to the nearest 0.5 µ m.

Record examination results for Cryptosporidium oocysts on a Cryptosporidium report examination results form. All oocysts that meet the criteria specified in Sections 15.2.2, less atypical organisms specifically identified as non target organisms by DIC or DAPI ( e. g. possessing spikes, stalks, appendages, pores, one or two large nuclei filling the cell, red fluorescing chloroplasts, crystals, spores, etc), must be reported. 15.2.1 Positive and negative staining control. Positive and negative staining controls must be acceptable. <IP_ADDRESS> Each analyst must characterize a minimum of three Cryptosporidium oocysts on the positive staining control slide before examining field sample slides. This characterization must be performed by each analyst during each microscope examination session. FITC examination must be conducted at a minimum of 200X total magnification, DAPI examination must be conducted at a minimum of 400X, and DIC examination and size measurements must be conducted at a minimum of 1000X. Size, shape, and DIC and DAPI characteristics of the three Cryptosporidium oocysts must be recorded by the analyst on a microscope log. The analyst also must indicate on each sample report examination results form whether the positive staining control was acceptable. <IP_ADDRESS> Examine the negative staining control to confirm that it does not contain any oocysts ( Section 14.1). Indicate on each sample report examination results form whether the negative staining control was acceptable. <IP_ADDRESS> If the positive staining control contains oocysts within the expected range and at the appropriate fluorescence for both FA and DAPI, and the negative staining control does not contain any oocysts ( Section 14.1), proceed to Sections 15.2.2. 15.2.2 Sample examination   Cryptosporidium <IP_ADDRESS> FITC examination ( the analyst must use a minimum of 200X total magnification). Use epifluorescence to scan the entire well for applegreen fluorescence of oocyst shapes. When brilliant apple green fluorescing ovoid or spherical objects 4 to 6 µ m in diameter are observed with brightly highlighted edges, increase magnification to 400X and switch the microscope to the UV filter block for DAPI ( Section <IP_ADDRESS>), then to DIC ( Section <IP_ADDRESS>) at 1000X. Method 1622  Cryptosporidium June 2003 51 <IP_ADDRESS> DAPI examination ( the analyst must use a minimum of 400X total magnification). Using the UV filter block for DAPI, the object will exhibit one of the following characteristics: ( a) Light blue internal staining ( no distinct nuclei) with a green rim ( b) Intense blue internal staining ( c) Up to four distinct, sky blue nuclei Record oocysts in category ( a) as DAPI negative; record oocysts in categories ( b) and ( c) as DAPI positive. <IP_ADDRESS> DIC examination ( the analyst must use a minimum of 1000X total magnification [ oil immersion lens]). Using DIC, look for external or internal morphological characteristics atypical of Cryptosporidium oocysts ( e. g., spikes, stalks, appendages, pores, one or two large nuclei filling the cell, red fluorescing chloroplasts, crystals, spores, etc.) ( adapted from Reference 20.7). If atypical structures are not observed, then categorize each apple green fluorescing object as: ( a) An empty Cryptosporidium oocyst ( b) A Cryptosporidium oocyst with amorphous structure ( c) A Cryptosporidium oocyst with internal structure ( one to four sporozoites/ oocyst) Using 1000X total magnification, record the shape, measurements ( to the nearest 0.5 µ m), and number of sporozoites ( if applicable) for each apple green fluorescing object meeting the size and shape characteristics. Although not a defining characteristic, surface oocyst folds may be observed in some specimens.

NOTE: All measurements must be made at 1000X magnification.

15.2.4 Record the date and time that sample examination was completed on the report examination results form. 15.2.5 Report Cryptosporidium concentrations as oocysts/ L. 15.2.6 Record analyst name 16.0 Analysis of Complex Samples 16.1 Some samples may contain high levels (> 1000/ L) of oocysts and/ or interfering organisms, substances, or materials. Some samples may clog the filter ( Section 12.0); others will not allow separation of the oocysts from the retentate or eluate; and others may contain materials that preclude or confuse microscopic examination. 16.2 If the sample holding time has not been exceeded and a full volume sample cannot be filtered, dilute an aliquot of sample with reagent water and filter this smaller aliquot ( Section 12.0). This dilution must be recorded and reported with the results. 16.3 If the holding times for the sample and for microscopic examination of the cleaned up retentate/ eluate have been exceeded, the site should be re sampled. If this is not possible, the results should be qualified accordingly.

17.0 Method Performance 17.1 Method acceptance criteria are shown in Tables 3 and 4 in Section 21.0. The initial and ongoing precision and recovery criteria are based on the results of spiked reagent water samples analyzed during the Information Collection Rule Supplemental Surveys ( Reference 20.8). The matrix spike and matrix spike duplicate criteria are based on spiked source water data generated during the interlaboratory validation study of Method 1623 involving 11 laboratories and 11 raw surface water matrices across the U. S. ( Reference 20.10). Method 1622  Cryptosporidium June 2003 52 NOTE: Some sample matrices may prevent the MS acceptance criteria in Table 3 to be met. An assessment of the distribution of MS recoveries across 430 MS samples from 87 sites during the ICR Supplemental Surveys is provided in Table 4.

18.0 Pollution Prevention 18.1 The solutions and reagents used in this method pose little threat to the environment when recycled and managed properly. 18.2 Solutions and reagents should be prepared in volumes consistent with laboratory use to minimize the volume of expired materials that need to be discarded to be disposed.

19.0 Waste Management 19.1 It is the laboratory's responsibility to comply with all federal, state, and local regulations governing waste management, particularly the biohazard and hazardous waste identification rules and land disposal restrictions, and to protect the air, water, and land by minimizing and controlling all releases from fume hoods and bench operations. Compliance with all sewage discharge permits and regulations is also required. An overview of these requirements can be found in the Environmental Management Guide for Small Laboratories ( EPA 233 B 98 001). 19.2 Samples, reference materials, and equipment known or suspected to have viable oocysts attached or contained must be sterilized prior to disposal. 19.3 For further information on waste management, consult The Waste Management Manual for Laboratory Personnel and Less is Better: Laboratory Chemical Management for Waste Reduction, both available from the American Chemical Society's Department of Government Relations and Science Policy, 1155 16th Street N. W., Washington, D. C. 20036.

20.0 References 20.1 Rodgers, Mark R., Flanigan, Debbie J., and Jakubowski, Walter, 1995. Applied and Environmental Microbiology 61 ( 10), 3759 3763. 20.2 Fleming, Diane O., et al.( eds.), Laboratory Safety: Principles and Practices, 2nd edition. 1995. ASM Press, Washington, DC 20.3 " Working with Carcinogens," DHEW, PHS, CDC, NIOSH, Publication 77 206, ( 1977). 20.4 " OSHA Safety and Health Standards, General Industry," OSHA 2206, 29 CFR 1910 ( 1976). 20.5 " Safety in Academic Chemistry Laboratories," ACS Committee on Chemical Safety ( 1979). 20.6 USEPA. Manual for the Certification of Laboratories Analyzing Drinking Water: Criteria and Procedures; Quality Assurance; Fourth Edition, EPA 815 B 97 001, Office of Ground Water and Drinking Water, U. S. Environmental Protection Agency, 26 West Martin Luther King Drive, Cincinnati, OH 45268 ( 1997). 20.7 ICR Microbial Laboratory Manual, EPA/ 600/ R 95/ 178, National Exposure Research Laboratory, Office of Research and Development, U. S. Environmental Protection Agency, 26 Martin Luther King Drive, Cincinnati, OH 45268 ( 1996). 20.8 USEPA. EPA Guide to Method Flexibility and Approval of EPA Water Methods, EPA 821 D 96 004. Office of Water, Engineering and Analysis Division, Washington, DC 20460 ( 1996). 20.8 Connell, K., C. C. Rodgers, H. L. Shank Givens, J Scheller, M. L Pope, and K. Miller, 2000. Building a Better Protozoa Data Set. Journal AWWA, 92: 10: 30. 20.9 " Envirochek   Sampling Capsule," PN 32915, Gelman Sciences, 600 South Wagner Road, Ann Arbor, MI 48103 9019 ( 1996). Method 1622  Cryptosporidium June 2003 53 20.10 USEPA. Results of the Interlaboratory Method Validation Study for Determination of Cryptosporidium and Giardia Using USEPA Method 1623, EPA 821 R 01 028. Office of Water, Office of Science and Technology, Engineering and Analysis Division, Washington, DC ( 2001). 20.11 " Dynabeads ® GC Combo," Dynal Microbiology R& D, P. O. Box 8146 Dep., 0212 Oslo, Norway ( September 1998, Revision no. 01). 20.12 USEPA. Implementation and Results of the Information Collection Rule Supplemental Surveys. EPA 815 R 01 003. Office of Water, Office of Ground Water and Drinking Water, Standards and Risk Management Division, Washington, DC ( 2001). 20.13 Connell, K., J. Scheller, K. Miller, and C. C. Rodgers, 2000. Performance of Methods 1622 and 1623 in the ICR Supplemental Surveys. Proceedings, American Water Works Association Water Quality Technology Conference, November 5  9, 2000, Salt Lake City, UT. Method 1622  Cryptosporidium June 2003 54 21.0 Tables and Figures Table 1. Method Holding Times ( See Section 8.2 for details)

Sample Processing Step Maximum Allowable Time between Breaks ( Samples should be processed as soon as possible)

Collection Filtration ' Up to 96 hours are permitted between sample collection ( if shipped to the laboratory as a bulk sample) or filtration ( if filtered in the field) and initiation of elution Elution These steps must be completed in 1 working day Concentration Purification Application of purified sample to slide Drying of sample ' Up to 72 hours are permitted from application of the purified sample to the slide to staining Staining ' Up to 7 days are permitted between sample staining and examination Examination Method 1622  Cryptosporidium June 2003 55 Table 2. Tier 1 and Tier 2 Validation/ Equivalency Demonstration Requirements Test Description Tier 1 modification( 1) Tier 2 modification( 2)

IPR ( Section 9.4) 4 replicates of spiked reagent water Required. Must be accompanied by a method blank. Required per laboratory Method blank ( Section 9.6) Unspiked reagent water Required Required per laboratory MS ( Section 9.5.1) Spiked matrix water Required on each water to which the modification will be applied and on every 20th sample of that water thereafter. Must be accompanied by an unspiked field sample collected at the same time as the MS sample Not required MS/ MSD ( Section 9.5) 2 replicates of spiked matrix water Recommended, but not required. Must be accompanied by an unspiked field sample collected at the same time as the MS sample Required per laboratory. Each laboratory must analyze a different water. ( 1) If a modification will be used only in one laboratory, these tests must be performed and the results must meet all of the QC acceptance criteria in the method ( these tests also are required the first time a laboratory uses the validated version of the method) ( 2) If nationwide approval of a modification is sought for one type of water matrix ( such as surface water), a minimum of 3 laboratories must perform the tests and the results from each lab individually must meet all QC acceptance criteria in the method. If more than 3 laboratories are used in a study, a minimum of 75% of the laboratories must meet all QC acceptance criteria.

NOTE: The initial precision and recovery and ongoing precision and recovery ( OPR) acceptance criteria listed in Table 3 are based on results from 293 Cryptosporidium OPR samples analyzed by six laboratories during the Information Collection Rule Supplemental Surveys ( Reference 20.13). The matrix spike acceptance criteria are based on data generated through interlaboratory validation of Method 1622 ( Reference 20.11).

Table 3. Quality Control Acceptance Criteria for Cryptosporidium Performance test Section Acceptance criteria Initial precision and recovery Mean recovery ( percent)

Precision ( as maximum relative standard deviation) 9.4 9.4.2 24  100 9.4.2 55 Ongoing precision and recovery ( percent) 9.7 11  100 Matrix spike/ matrix spike duplicate ( for method modifications) 9.5 Mean recovery1, 2 ( as percent) 9.5.2 13  143 Precision ( as maximum relative percent difference) 9.5.2 67 ( 1) The acceptance criteria for mean MS/ MSD recovery serves as the acceptance criteria for MS recovery during routine use of the method ( Section 9.5.1). ( 2) Some sample matrices may prevent the acceptance criteria from being met. An assessment of the distribution of MS recoveries from multiple MS samples from 87 sites during the ICR Supplemental Surveys is provided in Table 4. Method 1622  Cryptosporidium June 2003 56 Table 4. Distribution of Matrix Spike Recoveries from Multiple Samples Collected from 87 Source Waters During the ICR Supplemental Surveys ( Adapted from Reference 20.14)

MS Recovery Range Percent of 430 Cryptosporidium MS Samples in Recovery Range < 10% 6.7%

> 10%  20% 6.3%

> 20%  30% 14.9%

> 30%  40% 14.2%

> 40%  50% 18.4%

> 50%  60% 17.4%

> 60%  70% 11.2%

> 70%  80% 8.4%

> 80%  90% 2.3%

> 90% 0.2% Method 1622  Cryptosporidium June 2003 57 A B E D C 1.

3. 4. 2. 1 mm 1/ 5 mm Figure 1. Hemacytometer Platform Ruling. Squares 1, 2, 3, and 4 are used to count stock suspensions of Cryptosporidium oocysts ( after Miale, 1967) Method 1622  Cryptosporidium June 2003 58 Figure 2. Manner of Counting Oocysts in 1 Square mm. Dark organisms are counted and light organisms are omitted ( after Miale, 1967). Method 1622  Cryptosporidium June 2003 59 Figure 3. Laboratory Filtration System Method 1622  Cryptosporidium June 2003 60 Figure 4. Methods for Scanning a Well Slide Method 1622  Cryptosporidium June 2003 61 22.0 Glossary of Definitions and Purposes These definitions and purposes are specific to this method but have been conformed to common usage as much as possible.

22.1 Units of weight and measure and their abbreviations 22.1.1 Symbols ° C degrees Celsius µ L microliter < less than > greater than % percent 22.1.2 Alphabetical characters cm centimeter g gram G acceleration due to gravity hr hour ID inside diameter in. inch L liter m meter MCS microscope cleaning solution mg milligram mL milliliter mm millimeter mM millimolar N normal; gram molecular weight of solute divided by hydrogen equivalent of solute, per liter of solution RSD relative standard deviation sr standard deviation of recovery X average percent recovery 22.2 Definitions, acronyms, and abbreviations ( in alphabetical order)

Analyst   The analyst should have at least 2 years of college in microbiology or equivalent or closely related field. The analyst also should have a minimum of 6 months of continuous bench experience with Cryptosporidium and IFA microscopy. The analyst shouldhave a minimum of 3 months experience using EPA Method 1622 and/ or EPA Method 1623 and should have successfully analyzed a minimum of 50 samples using EPA Method 1622 and/ or EPA Method 1623. The analyst must have at least 2 years of college lecture and laboratory course work in microbiology or a closely related field. The analyst also must have at least 6 months of continuous bench experience with environmental protozoa detection techniques and IFA microscopy, and must have successfully analyzed at least 50 water and/ or wastewater samples for Cryptosporidium. Six months of additional experience in the above areas may be substituted for two years of college.

Analyte   A protozoan parasite tested for by this method. The analyte in this method is Cryptosporidium.

Flow cytometer   A particle sorting instrument capable of counting protozoa. Method 1622  Cryptosporidium June 2003 62 Immunomagnetic separation ( IMS)   A purification procedure that uses microscopic, magnetically responsive particles coated with an antibodies targeted to react with a specific pathogen in a fluid stream. Pathogens are selectively removed from other debris using a magnetic field.

Initial precision and recovery ( IPR)   Four aliquots of spiking suspension analyzed to establish the ability to generate acceptable precision and accuracy. An IPR is performed prior to the first time this method is used and any time the method or instrumentation is modified.

Laboratory blank   See Method blank Laboratory control sample ( LCS)   See Ongoing precision and recovery ( OPR) standard Matrix spike ( MS)   A sample prepared by adding a known quantity of organisms to a specified amount of sample matrix for which an independent estimate of target analyte concentration is available. A matrix spike is used to determine the effect of the matrix on a method's recovery efficiency.

May   This action, activity, or procedural step is neither required nor prohibited.

May not   This action, activity, or procedural step is prohibited.

Method blank   An aliquot of reagent water that is treated exactly as a sample, including exposure to all glassware, equipment, solvents, and procedures that are used with samples. The method blank is used to determine if analytes or interferences are present in the laboratory environment, the reagents, or the apparatus.

Must   This action, activity, or procedural step is required.

Negative control   See Method blank Nucleus   A membrane bound organelle containing genetic material. Nuclei are a prominent internal structure seen both in Cryptosporidium oocysts. In Cryptosporidium oocysts, there is one nucleus per sporozoite.

Oocyst   The encysted zygote of some sporozoa; e. g., Cryptosporidium. The oocyst is a phase or form of the organism produced as a normal part of the life cycle of the organism. It is characterized by a thick and environmentally resistant outer wall.

Ongoing precision and recovery ( OPR) standard   A method blank spiked with known quantities of analytes. The OPR is analyzed exactly like a sample. Its purpose is to assure that the results produced by the laboratory remain within the limits specified in this method for precision and recovery.

Oocyst spiking suspension   See Spiking suspension Oocyst stock suspension   See Stock suspension Positive control   See Ongoing precision and recovery standard Principle analyst   The principle analyst ( may not be applicable to all monitoring programs) should have a BS/ BA in microbiology or closely related field and a minimum of 1 year of continuous bench experience with Cryptosporidium and IFA microscopy. The principle analyst also should have a minimum of 6 months experience using EPA Method 1622 and/ or EPA Method 1622  Cryptosporidium June 2003 63 Method 1623 and should have analyzed a minimum of 100 samples using EPA Method 1622 and/ or EPA Method 1623.

PTFE   Polytetrafluoroethylene Quantitative transfer   The process of transferring a solution from one container to another using a pipette in which as much solution as possible is transferred, followed by rinsing of the walls of the source container with a small volume of rinsing solution ( e. g., reagent water, buffer, etc.), followed by transfer of the rinsing solution, followed by a second rinse and transfer.

Reagent water   Water demonstrated to be free from the analytes of interest and potentially interfering substances at the method detection limit for the analyte.

Reagent water blank   see Method blank Relative standard deviation ( RSD)   The standard deviation divided by the mean times 100.

RSD   See Relative standard deviation Should   This action, activity, or procedural step is suggested but not required.

Spiking suspension   Diluted stock suspension containing the organism( s) of interest at a concentration appropriate for spiking samples.

Sporozoite   A motile, infective stage of certain protozoans; e. g., Cryptosporidium. There are four sporozoites in each Cryptosporidium oocyst, and they are generally banana shaped.

Stock suspension   A concentrated suspension containing the organism( s) of interest that is obtained from a source that will attest to the host source, purity, authenticity, and viability of the organism( s).


[Federal Register Volume 87, Number 70 (Tuesday, April 12, 2022)]
[Notices]
[Pages 21649-21650]
[FR Doc No: 2022-07804]

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DEPARTMENT OF ENERGY

Federal Energy Regulatory Commission

[Docket No. DI22-1-000]

Donald Higgins; Notice of Declaration of Intention and Soliciting
Comments, Protests, and Motions To Intervene

    Take notice that the following application has been filed with the
Commission and is available for public inspection:
    a. Application Type: Declaration of Intention.
    b. Docket No: DI22-1-000.
    c. Date Filed: November 1, 2021.
    d. Applicant: Donald Higgins.
    e. Name of Project: Hydrokinetic Floating Generator.
    f. Location: The proposed Hydrokinetic Floating Generator would be
located on the Rocky River near the town of Concord, in Cabarrus
County, North Carolina.
    g. Filed Pursuant to: Section 23(b)(1) of the Federal Power Act, 16
U.S.C. 817(b).
    h. Applicant Contact: Donald Higgins; 3721 Mill Bridge Rd.,
Concord, NC 28025; telephone:<PHONE_NUMBER>; email: [email protected].
    i. FERC Contact: Jennifer Polardino,<PHONE_NUMBER>, or
[email protected].
    j. Deadline for filing comments, protests, and motions to intervene
is: May 6, 2022.
    The Commission strongly encourages electronic filing. Please file
comments, protests, and motions to intervene using the Commission's
eFiling system at http://www.ferc.gov/docs-filing/efiling.asp.
Commenters can submit brief comments up to 6,000 characters, without
prior registration, using the eComment system at http://www.ferc.gov/docs-filing/ecomment.asp. You must include your name and contact
information at the end of your comments. For assistance, please contact
FERC Online Support at [email protected],<PHONE_NUMBER> (toll
free), or<PHONE_NUMBER> (TTY). In lieu of electronic filing, you may
submit a paper copy. Submissions sent via the U.S. Postal Service must
be addressed to: Kimberly D. Bose, Secretary, Federal Energy Regulatory
Commission, 888 First Street NE, Room 1A, Washington, DC 20426.
Submissions sent via any other carrier must be addressed to: Kimberly
D. Bose, Secretary, Federal Energy Regulatory Commission, 12225 Wilkins
Avenue, Rockville, Maryland 20852. The first page of any filing should
include docket number DI22-1-000. Comments emailed to Commission staff
are not considered part of the Commission record.
    k. Description of Project: The proposed Hydrokinetic Floating
Generator would consist of: (1) An anchored floating platform supported
by two pontoons; (2) two partially immersed paddle wheels mounted on
the platform; (3) a drive train consisting of a chain and sprockets,
intermediate shaft, and a belt and pulleys; (4) a 12-volt, 400-watt
generator running between 60 and 180 revolutions per minute; (5) a
transmission line to a private residence; and (6) appurtenant
facilities.
    When a Declaration of Intention is filed with the Federal Energy
Regulatory Commission, the Federal Power Act requires the Commission to
investigate and determine if the project would affect the interests of
interstate or foreign commerce. The Commission also determines whether
or not the project: (1) Would be located on a navigable waterway; (2)
would occupy public lands or reservations of the United States; (3)
would utilize surplus water or water power from a government dam; or
(4) would be located on a non-navigable stream over which Congress has
Commerce Clause jurisdiction and would be constructed or enlarged after
1935.
    l. Locations of the Application: This filing may be viewed on the
Commission's website at http://www.ferc.gov/docs-filing/elibrary.asp.
Enter the docket number excluding the last three digits in the docket
number field to access the document. You may also register online at
http://www.ferc.gov/docs-filing/esubscription.asp to be notified via
email of new filings and issuances related to this or other pending
projects. For assistance, call 1-866-208-3676 or email
[email protected], for TTY, call<PHONE_NUMBER>.
    m. Individuals desiring to be included on the Commission's mailing
list should so indicate by writing to the Secretary of the Commission.
    n. Comments, Protests, or Motions to Intervene: Anyone may submit
comments, a protest, or a motion to intervene in accordance with the
requirements of Rules of Practice and Procedure, 18 CFR 385.210, .211,
and .214. In determining the appropriate action to take, the Commission
will consider all protests or other comments filed, but only those who
file a motion to intervene in accordance with the Commission's Rules
may become a party to the proceeding. Any comments, protests, or
motions to intervene must be received on or before the specified

comment date for the particular application.
    o. Filing and Service of Responsive Documents: All filings must
bear in all capital letters the title ``COMMENTS'', ``PROTESTS'', and
``MOTIONS TO INTERVENE'', as applicable, and the Docket Number of the
particular application to which the filing refers. A copy of any Motion
to Intervene must also be served upon each representative of the
Applicant specified in the particular application.
    p. Agency Comments: Federal, state, and local agencies are invited
to file comments on the described application. A copy of the
application may be obtained by agencies directly from the Applicant. If
an agency does not file comments within the time specified for filing
comments, it will be presumed to have no comments. One copy of an
agency's comments must also be sent to the Applicant's representatives.

    Dated: April 6, 2022.
Kimberly D. Bose,
Secretary.
[FR Doc. 2022-07804 Filed 4-11-22; 8:45 am]
BILLING CODE 6717-01-P


BILLING CODE 6560-50-P
ENVIRONMENTAL PROTECTION AGENCY
[EPA-HQ-OPP-2021-0346; FRL-10023-90]
Agency Information Collection Activities; Proposed Renewal of an Existing Collection and Request for Comment; Labeling Requirements for Certain Minimum Risk Pesticides under FIFRA Section 25(b)
AGENCY: Environmental Protection Agency (EPA).
ACTION: Notice.
SUMMARY: In compliance with the Paperwork Reduction Act (PRA), this document announces the availability of and solicits public comment on an Information Collection Request (ICR) that EPA is planning to submit to the Office of Management and Budget (OMB). The ICR, entitled: "Labeling Requirements for Certain Minimum Risk Pesticides under FIFRA Section 25(b)" and identified by EPA ICR No. 2475.04and OMB Control No. 2070-0187, represents the renewal of an existing ICR that is schedules to expire on January 2022. Before submitting the ICR to OMB for review and approval under the PRA, EPA is soliciting comments on specific aspects of the proposed information collection that is summarized in this document. The ICR and accompanying material are available in the docket for public review and comment.
DATES: Comments must be received on or before August 29, 2021.
 ADDRESSES: Submit your comments, identified by docket identification (ID) number EPA-HQ-OPP-2021-0345, by one of the following methods:
 	:: Federal eRulemaking Portal: http://www.regulations.gov. Follow the online instructions for submitting comments. Do not submit electronically any information you consider to be Confidential Business Information (CBI) or other information whose disclosure is restricted by statute.
	:: Mail: OPP Docket, Environmental Protection Agency Docket Center (EPA/DC), (28221T), 1200 Pennsylvania Ave., NW., Washington, DC 20460-0001.
	:: Hand Delivery: To make special arrangements for hand delivery or delivery of boxed information, please follow the instructions at http://www.epa.gov/dockets/contacts.html.
	Due to the public health concerns related to COVID-19, the EPA Docket Center (EPA/DC) and Reading Room is closed to visitors with limited exceptions. The staff continues to provide remote customer service via email, phone, and webform. For the latest status information on EPA/DC services and docket access, visit https://www.epa.gov/dockets.
	Additional instructions on commenting or visiting the docket, along with more information about dockets generally, is available at http://www.epa.gov/dockets.
FOR FURTHER INFORMATION CONTACT: Nora Stoner, MSD (6560-50-P), Office of Chemical Safety and Pollution Prevention, Environmental Protection Agency, 1200 Pennsylvania Ave., NW., Washington, DC 20460-0001; telephone number:<PHONE_NUMBER>5; email address<EMAIL_ADDRESS>SUPPLEMENTARY INFORMATION:
I. What Information is EPA Particularly Interested in?
	Pursuant to PRA section 3506(c)(2)(A) (44 U.S.C. 3506(c)(2)(A)), EPA specifically solicits comments and information to enable it to:
	1. Evaluate whether the proposed collection of information is necessary for the proper performance of the functions of the Agency, including whether the information will have practical utility.
	2. Evaluate the accuracy of the Agency's estimates of the burden of the proposed collection of information, including the validity of the methodology and assumptions used.
	3. Enhance the quality, utility, and clarity of the information to be collected.
	4. Minimize the burden of the collection of information on those who are to respond, including through the use of appropriate automated electronic, mechanical, or other technological collection techniques or other forms of information technology, e.g., permitting electronic submission of responses. In particular, EPA is requesting comments from very small businesses (those that employ less than 25) on examples of specific additional efforts that EPA could make to reduce the paperwork burden for very small businesses affected by this collection.
II. What Information Collection Activity or ICR Does this Action Apply to?
	Title: Labeling Requirements for Certain Minimum Risk Pesticides under FIFRA Section 25(b).
	ICR number: EPA ICR No. 2475.04.
	OMB control number: OMB Control No. 2070-0187.
	ICR status: This ICR is currently scheduled to expire on January 2022. An Agency may not conduct or sponsor, and a person is not required to respond to, a collection of information, unless it displays a currently valid OMB control number. The OMB control numbers for EPA's regulations in title 40 of the Code of Federal Regulations (CFR), after appearing in the Federal Register when approved, are listed in 40 CFR part 9, are displayed either by publication in the Federal Register or by other appropriate means, such as on the related collection instrument or form, if applicable. The display of OMB control numbers for certain EPA regulations is consolidated in 40 CFR part 9.
	Abstract: Minimum risk pesticide products are not registered by EPA, therefore the product information associated with the pesticide registration process under section 3 of FIFRA are never submitted to EPA. Labeling requirements are the key component of the minimum risk exemption since this is the only information that enforcement authorities have to assess whether or not the product meets the exemption requirements. While EPA does not review these products, and therefore a Federal label review is not conducted, to maintain exemption status, an exempt product's label must meet certain criteria. The Agency will reach out as required under 5 CFR 1320.8(d)(1), to representatives from producers of minimum risk pesticide products that would be subjected to the labeling requirements under 40 CFR 152.25(f).
	Burden statement: The annual public reporting and recordkeeping burden for this collection of information is estimated to average 5.5 hours per response. Burden is defined in 5 CFR 1320.3(b).
	The ICR, which is available in the docket along with other related materials, provides a detailed explanation of the collection activities and the burden estimate that is only briefly summarized here:
	Respondents/Affected Entities: Entities potentially affected by this ICR are individuals or entities engaged in activities related to the registration of pesticide products including manufacturers, distributers, retailers, and users of minimum risk pesticides.
	Respondent's obligation to respond: Voluntary.
	Estimated total number of potential respondents: 7.
	Frequency of response: One time.
	Estimated total average number of responses for each respondent: 87.
	Estimated total annual burden hours: 478.5 hours.
	Estimated total annual costs: $ 61,018.45. This includes an estimated burden cost of $ 50,576.67 and an estimated cost of $ 9,963.28 for capital investment or maintenance and operational costs.

[III]. Are There Changes in the Estimates from the Last Approval?
	There is no change of hours in the total estimated respondent burden compared with that
[Insert appropriate Roman numeral, depending on whether this is a renewal ("V.") or a new ICR]
[IV]. What is the Next Step in the Process for this ICR?
	EPA will consider the comments received and amend the ICR as appropriate. The final ICR package will then be submitted to OMB for review and approval pursuant to 5 CFR 1320.12. EPA will issue another Federal Register document pursuant to 5 CFR 1320.5(a)(1)(iv) to announce the submission of the ICR to OMB and the opportunity to submit additional comments to OMB. If you have any questions about this ICR or the approval process, please contact the person listed under FOR FURTHER INFORMATION CONTACT.
	Authority: 44 U.S.C. 3501 et seq.
Dated: Click or tap to enter eSignature date.
Michal Freedhoff,
Acting Assistant Administrator, Office of Chemical Safety and Pollution Prevention.


[Federal Register: January 29, 2008 (Volume 73, Number 19)]
[Notices]
[Page 5183-5184]
[DOCID:fr29ja08-49]

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DEPARTMENT OF ENERGY

Federal Energy Regulatory Commission

Combined Notice of Filings 1

January 23, 2008.
    Take notice that the Commission has received the following Natural
Gas Pipeline Rate and Refund Report filings:
    Docket Numbers: RP97-255-080.
    Applicants: TransColorado Gas Transmission Company.
    Description: TransColorado Gas Transmission Co. submits Eighteenth
Revised Sheet 21 and Fourteenth Revised Sheet 22B to FERC Gas Tariff,
First Revised Volume 1 etc., in compliance with FERC's 11/29/07 Order.
    Filed Date: 01/17/2008.
    Accession Number: 20080123-0025.
    Comment Date: 5 p.m. Eastern Time on Tuesday, January 29, 2008.

    Docket Numbers: RP97-255-080.
    Applicants: TransColorado Gas Transmission Company.
    Description: TransColorado Gas Transmission Co., LLC submits marked
version of the 1/17/18 filing of two agreements with EnCana Marketing
USA, Inc in compliance with FERC's 11/29/07 Order.
    Filed Date: 01/18/2008.
    Accession Number: 20080123-0024.
    Comment Date: 5 p.m. Eastern Time on Wednesday, January 30, 2008.

    Docket Numbers: RP99-176-150.
    Applicants: Natural Gas Pipeline Company of America.
    Description: Natural Gas Pipeline Company of America submits
Amendment 1 to the Firm Transportation and Storage Negotiated Rate
Agreement with CenterPoint Energy Minnesota Gas.
    Filed Date: 01/18/2008.
    Accession Number: 20080123-0022.
    Comment Date: 5 p.m. Eastern Time on Wednesday, January 30, 2008.

    Docket Numbers: RP99-301-199.
    Applicants: ANR Pipeline Company.
    Description: ANR Pipeline Company submits amendments to an Amended
and Restated Minimum Delivery Pressure and Maximum Delivery Level
Agreement with Wisconsin Electric Power Company.
    Filed Date: 01/18/2008.
    Accession Number: 20080123-0023.
    Comment Date: 5 p.m. Eastern Time on Wednesday, January 30, 2008.

    Docket Numbers: RP05-157-009.
    Applicants: Saltville Gas Storage Company L.L.C.
    Description: Saltville Gas Storage Company, LLC submits Sub
Original Sheet 20 et al. to FERC Gas Tariff, Original Volume 1, to be
effective 2/18/08.
    Filed Date: 01/18/2008.
    Accession Number: 20080123-0021.
    Comment Date: 5 p.m. Eastern Time on Wednesday, January 30, 2008.

    Docket Numbers: RP05-422-025.
    Applicants: El Paso Natural Gas Company.
    Description: El Paso Natural Gas Co's CD containing its refund
report.
    Filed Date: 01/18/2008.
    Accession Number: 20080118-4013.
    Comment Date: 5 p.m. Eastern Time on Wednesday, January 30, 2008.

    Docket Numbers: RP07-328-001.
    Applicants: Transcontinental Gas Pipe Line Corporation.
    Description: Transcontinental Gas Pipe Line Corporation tendered
for filing a compliance filing to the Commission's order issued on
March 29, 2007 in the above referenced proceeding, concerning the
``Eminence Storage Loss'' reflected in Transcontinental's March 1, 2007
filing.
    Filed Date: 04/30/2007.
    Accession Number: 20070430-5025.
    Comment Date: 5 p.m. Eastern Time, Tuesday, January 29, 2008.

    Docket Numbers: RP08-109-002.
    Applicants: Northern Natural Gas Company.
    Description: Northern Natural Gas Company submits a filing
proposing additional Firm Deferred Delivery Storage Flexibility by
allowing shippers to reduce their scheduled firm withdrawal & injection
quantities etc.
    Filed Date: 01/18/2008.
    Accession Number: 20080123-0020.
    Comment Date: 5 p.m. Eastern Time on Wednesday, January 30, 2008.

    Docket Numbers: RP08-164-000.
    Applicants: Florida Gas Transmission Company, LLC.
    Description: Florida Gas Transmission Company submits First Revised
Sheet 650 et al. to its FERC Gas Tariff, Fourth Revised Volume 1, to
become effective 2/16/08.
    Filed Date: 01/16/2008.
    Accession Number: 20080117-0038.
    Comment Date: 5 p.m. Eastern Time on Tuesday, January 29, 2008.

    Docket Numbers: RP08-167-000.
    Applicants: TransColorado Gas Transmission Company.
    Description: TransColorado Gas Transmission Co, LLC submits
Original Sheet 0 and 1 et al. to its FERC Gas Tariff, Second Revised
Volume 1.
    Filed Date: 01/18/2008.
    Accession Number: 20080123-0018.
    Comment Date: 5 p.m. Eastern Time on Wednesday, January 30, 2008.

    Docket Numbers: RP08-168-000.

    Applicants: Trailblazer Pipeline Company.
    Description: Trailblazer Pipeline Company submits Original Sheet 0
and 1 et al. to FERC Gas Tariff, Fourth Revised Volume 1, to be
effective 12/28/07.
    Filed Date: 01/18/2008.
    Accession Number: 20080123-0026.
    Comment Date: 5 p.m. Eastern Time on Wednesday, January 30, 2008.

    Docket Numbers: RP08-169-000.
    Applicants: Dominion Cove Point LNG, LP.
    Description: Dominion Cove Point LNG, LP submits Eighth Revised
Sheet 11 to its FERC Gas Tariff, Original Volume 1, to be effective 2/
17/08.
    Filed Date: 01/18/2008.
    Accession Number: 20080123-0019.
    Comment Date: 5 p.m. Eastern Time on Wednesday, January 30, 2008.
    Any person desiring to intervene or to protest in any of the above
proceedings must file in accordance with Rules 211 and 214 of the
Commission's Rules of Practice and Procedure (18 CFR 385.211 and
385.214) on or before 5 p.m. Eastern time on the specified comment
date. It is not necessary to separately intervene again in a subdocket
related to a compliance filing if you have previously intervened in the
same docket. Protests will be considered by the Commission in
determining the appropriate action to be taken, but will not serve to
make protestants parties to the proceeding. Anyone filing a motion to
intervene or protest must serve a copy of that document on the
Applicant. In reference to filings initiating a new proceeding,
interventions or protests submitted on or before the comment deadline
need not be served on persons other than the Applicant.
    The Commission encourages electronic submission of protests and
interventions in lieu of paper, using the FERC Online links at http://www.ferc.gov.
 To facilitate electronic service, persons with Internet

access who will eFile a document and/or be listed as a contact for an
intervenor must create and validate an eRegistration account using the
eRegistration link. Select the eFiling link to log on and submit the
intervention or protests.
    Persons unable to file electronically should submit an original and
14 copies of the intervention or protest to the Federal Energy
Regulatory Commission, 888 First St. NE., Washington, DC 20426.
    The filings in the above proceedings are accessible in the
Commission's eLibrary system by clicking on the appropriate link in the
above list. They are also available for review in the Commission's
Public Reference Room in Washington, DC. There is an eSubscription link
on the Web site that enables subscribers to receive e-mail notification
when a document is added to a subscribed dockets(s). For assistance
with any FERC Online service, please e-mail<EMAIL_ADDRESS>
or call<PHONE_NUMBER> (toll free). For TTY, call<PHONE_NUMBER>.

Nathaniel J. Davis, Sr.,
Deputy Secretary.
 [FR Doc. E8-1513 Filed 1-28-08; 8:45 am]

BILLING CODE 6717-01-P