1223.10 2 N/ A 223.60 27726.40 ****** 2247.88 GENERIC EECs ( IN MICROGRAMS/ LITER ( PPB)) Version 2.0 Aug 1, 2001

PEAK MAX 4 DAY MAX 21 DAY MAX 60 DAY MAX 90 DAY GEEC AVG GEEC AVG GEEC AVG GEEC AVG GEEC

33.89 33.88 33.83 33.70 33.61 RUN No. 2 FOR dimethipin ON apple * INPUT VALUES *

RATE (#/ AC) No. APPS & SOIL SOLUBIL APPL TYPE NO SPRAY INCORP ONE( MULT) INTERVAL Kd ( PPM ) (% DRIFT) ZONE( FT) ( IN)

.077( .077) 1 1 .0 4600.0 AERL_ B( 13.0) .0 .0  53 FIELD AND STANDARD POND HALFLIFE VALUES ( DAYS)

METABOLIC DAYS UNTIL HYDROLYSIS PHOTOLYSIS METABOLIC COMBINED ( FIELD) RAIN/ RUNOFF ( POND) ( POND EFF) ( POND) ( POND)

1223.10 2 N/ A 223.60 27726.40 ****** 2247.88 GENERIC EECs ( IN MICROGRAMS/ LITER ( PPB)) Version 2.0 Aug 1, 2001

PEAK MAX 4 DAY MAX 21 DAY MAX 60 DAY MAX 90 DAY GEEC AVG GEEC AVG GEEC AVG GEEC AVG GEEC

4.66 4.66 4.65 4.63 4.62  54 Appendix C. ELL Fate Model and Results The model output from ELL FATE for dimethipin is in the attached Microsoft Excel spreadsheet " Dimethipin ELLFATE 1.4".

Kenaga Estimates & ELL FATE Model   Explanation Hoerger and Kenaga estimates ( 1972) as modified by Fletcher and other researchers ( 1994) were used to approximate the residues on plants and insects. Hoerger Kenaga categories represent preferred foods of various terrestrial vertebrates: fruits and bud and shoot tips of leafy crops are preferred by upland game birds; leaves and stems of leafy crops are consumed by hares and hoofed mammals; seeds, seed pods and grasses are consumed by rodents; and insects are consumed by various birds, mammals, reptiles and terrestrial phase amphibians. Terrestrial vertebrates also may be exposed to pesticides applied to soil by ingesting pesticide granules and/ or pesticide laden soil when foraging. Rich in minerals, soil comprises 5 to 30% of dietary intake by many wildlife species ( Beyer and Conner, 1994).

Hoerger Kenaga pesticide environmental concentration estimates were based on residue data correlated from more than 20 pesticides on more than 60 crops. Representative of many geographic regions ( 7 states) and a wide array of cultural practices, Hoerger Kenaga estimates also considered differences in vegetative yield, surface/ mass ratio, and interception factors. In 1994, Fletcher, Nellessen, and Pfleeger reexamined the Hoerger Kenaga simple linear model ( y= B1x, where x= application rate and y= pesticide residue in ppm) to determine whether the terrestrial EEC's were accurate. They compiled a data set of pesticide day 0 and residue decay data involving 121 pesticides ( 85 insecticides, 27 herbicides, and 9 fungicides from 17 different chemical classes) on 118 species of plants. After analyses, their conclusions were that Hoerger Kenaga estimates needed only minor modifications to elevate the predictive values for forage and fruit categories from 58 to 135 ppm and from 7 to 15 ppm, respectively. Otherwise, the Hoerger Kenaga estimates were accurate in predicting the maximum residue values after a 1 lb ai/ A application. Mean values represent the arithmetic mean of values from samples collected the day of pesticide treatment. These values, in the table below, are the predicted 0 day maximum and mean residues of a pesticide that may be expected to occur on selected avian, mammalian, reptilian, or terrestrial phase amphibian food items immediately following a direct single application at a 1 lb ai/ A application rate. For pesticides applied as a nongranular product ( e. g., liquid, dust), the estimated environmental concentrations ( EECs) on food items following product application are compared to LC50 values to assess risk.

Spreadsheet based Terrestrial Exposure Values A first order decay assumption is used to determine the concentration at each day after initial application based on the concentration resulting from the initial and additional applications. The decay is calculated from the first order rate equation:  55 CT = Cie kT or in integrated form:

ln ( CT/ Ci) =  kT Where:

CT = concentration at time T on day zero.

Ci = concentration in parts per million ( ppm) present initially ( on day zero) on the surfaces.

Ci = is calculated based on Kenaga and Fletcher by multiplying the application rate, in pounds active ingredient per acre, by 240 for short grass, 110 for tall grass, 135 for broad leaf plants/ insects, and 15 for seeds. Additional applications are converted from pounds active ingredient per acre to ppm on the plant surface and the addition mass added to the mass of the chemical still present on the surfaces on the day of application.

k = degradation rate constant determined from studies of hydrolysis, photolysis, microbial degradation, etc. Since degradation rate is generally reported in terms of half life, the rate constant is calculated from the input half life ( k = ln 2/ T1/ 2) instead of being input directly. Choosing which process controls the degradation rate and which half life to use in terrestrial exposure calculations is open for debate and should be done by a qualified scientist.

T = time, in days, since the start of the simulation. The initial application is on day 0. The simulation is set to run for 365 days.

The program calculates concentration on each type of surface on a daily interval for one year. The maximum concentration during the year and the average concentration during the first 56 days are calculated.  56 Appendix D. TerrPlant Model and Results TerrPlant was not run for this risk assessment because terrestrial plant toxicity data valid for these calculations were unavailable.  57 Appendix E. Ecological Effects Data 1. Overview The toxicity testing required does not test all species of birds, fish, mammals, invertebrates, and plants. Only two surrogate species for birds ( bobwhite quail and mallard ducks) are used to represent all bird species ( over 900 in the U. S.), three species of freshwater fish ( rainbow trout, bluegill sunfish, and fathead minnow) are used to represent all freshwater fish species ( over 900 in the U. S.), and one estuarine/ marine fish species ( sheepshead minnow) is used to represent all estuarine/ marine fish ( over 300 in the U. S.). The surrogate species for terrestrial invertebrates is the honey bee, for freshwater invertebrates the surrogate species is usually the water flea ( Daphnia magna) and for estuarine/ marine invertebrates the surrogate species are mysid shrimp and eastern oyster. These five species are used to represent all invertebrates species ( over 10,000 in the U. S.). For plants, there are ten surrogate species used for all terrestrial plants and five surrogate species used for all aquatic plants. There are over 20,000 plant species in the U. S. which includes flowering plants, conifers, ferns, mosses, liverworts, hornworts, and lichens with over 27,000 species of algae worldwide.

The surrogate species testing scheme used in this assessment assumes that a chemical's mechanism of action and toxicity found for avian species is similar to that in all reptiles ( over 300 species in the U. S.). The same assumption applies to amphibians ( over 200 species in the U. S.) and fish; the tadpole stage of amphibians is assumed to have the same sensitivity as a fish. Therefore, the results from toxicity tests on surrogate species are considered applicable to other member species within their class and are extrapolated to reptiles and amphibians. The U. S. species numbers noted in this section were taken from: http:// www. natureserve. org/ summary ( NatureServe: An online encyclopedia of life [ web application], 2000) and the worldwide species number from Ecological Planning and Toxicology, Inc., 1996.

2. Toxicity to Terrestrial Animals a. Birds: Acute and Subacute One acute oral toxicity study using TGAI is required to establish the toxicity of dimethipin to birds. The preferred test species are mallard duck ( a waterfowl) or bobwhite quail ( an upland game bird). Results of an acute exposure study in mallard ducks study are tabulated below in Table E 1. Since the LD50 value of 880 mg a. i./ kg is in the range of 501   2,000 mg/ kg, dimethipin is categorized as slightly toxic to avian species on an acute oral basis. Data submitted in support of the avian acute oral toxicity test requirement, Guideline 71 1( a) ( Acute Avian Oral, Quail/ Duck), are considered useful for purposes of risk assessment ( MRID 41955901).  58 Table E 1. Avian acute oral toxicity.

Species % a. i. LD50 ( mg a. i./ kg) Toxicity Category Identification Number Study Classification1 Mallard ducks ( Anus platyrhynchos) 98.64 ( Harvade Technical, administered by gelatin capsule) 880 Effects noted: decreased body weight and food consumption Slightly toxic MRID 41955901 ( Pedersen 1991) Core 1Core: study satisfies guideline; Supplemental: study is scientifically sound, but does not satisfy guideline; Invalid: study is not scientifically sound and does not satisfy guideline.

Categories of acute toxicity for avian species can be classified according to the toxicity reference value ( LC50) given by a study ( EPA 2001):

LC50 ( ppm) Toxicity Category < 50 Very highly toxic 50   500 Highly toxic 501   1000 Moderately toxic 1001   5000 Slightly toxic > 5000 Practically nontoxic Two subacute dietary studies using the TGAI are required to establish the toxicity of dimethipin to birds. The preferred test species are mallard duck ( Guideline 71 2( b), Acute Avian Diet, Duck) and bobwhite quail ( Guideline 71 2( a), Acute Avian Diet, Quail). Results of these tests are tabulated below in Table E 2. The data that were submitted for both mallard ducks and bobwhite quail show that the LC50 is > 5,000 ppm a. i.; therefore, dimethipin is categorized as practically non toxic to avian species on a subacute dietary basis. The data submitted under Guideline 71 2( b) are considered useful for purposes of quantifying risks to avian species ( MRID 41955902). However, the study in bobwhite quail was classified as supplemental because the test material was not homogeneously incorporated into the basal feed ( MRID 41945901). Therefore, the data on the bobwhite quail study are considered less representative than the data  59 on the mallard duck for purposes of risk assessment.

Table E 2. Avian subchronic dietary toxicity.

Species % a. i. LD50 ( ppm a. i.) 1 Toxicity Category Identification Number Study Classification2 Mallard ducks ( Anus platyrhynchos) 98.64 ( Harvade Technical) LC50 > 5,000 ( no mortality)

NOAEC ( for decreased body weight) 1,250 Practically non toxic MRID 41955902 ( Pedersen 1991) Core Bobwhite quail ( Colinus virginianus) 98.64 ( Harvade technical) LC50 > 5,000 ( measured concentration 6,046)

NOAEC ( for mortality and signs of toxicity) 5,000 ( measured concentration in food 6,046)

LOAEC ( for mortality and signs of toxicity) > 5,000 ( measured concentration in food 6,046 Practically non toxic MRID 41945901 ( Pedersen 1991) Supplemental ( classified as supplemental because test material was not homogeneously incorporated in the basal feed)

15 day dietary exposure followed by additional 3 day observation period.

2Core: study satisfies guideline; Supplemental: study is scientifically sound, but does not satisfy guideline; Invalid: study is not scientifically sound and does not satisfy guideline.

b. Birds: Chronic  60 Avian reproduction studies using the TGAI are required for dimethipin when one or more of the following conditions are met: ( 1) birds may be subject to repeated or continuous exposure to the pesticide, especially preceding or during the mating season, ( 2) the pesticide is stable in the environment to the extent that potentially toxic amounts may persist in animal feed, ( 3) the pesticide is stored or accumulated in plant or animal tissues, and/ or ( 4) information derived from mammalian reproduction studies indicates reproduction in terrestrial vertebrates may be adversely affected by the anticipated use of the product. For dimethipin, The preferred test species are mallard duck and bobwhite quail. No data were submitted under Guidelines 71 4( a) Avian Reproduction, Quail and 71 4( b) Avian Reproduction, Duck., so chronic risks to avian species could not be assessed.

c. Mammals: Acute and Chronic In general, categories of acute toxicity for mammalian species can be classified according to the toxicity reference value ( LD50) given by a study ( EPA 2001):

LD50 ( mg a. i./ kg) Toxicity Category < 10 Very highly toxic 10   50 Highly toxic 51   500 Moderately toxic 501   2000 Slightly toxic > 2000 Practically nontoxic Wild mammal testing is required on a case by case basis, depending on the results of lower tier laboratory mammalian studies, intended use pattern, and pertinent environmental fate characteristics. In most cases, rat or mouse toxicity values obtained from the Agency's Health Effects Division ( HED) substitute for wild mammal testing. These toxicity values are reported below in Table E 3. Based on these results, dimethipin is categorized as moderately toxic to small mammals on an acute oral basis. Although HED did not conduct an acute aggregate risk assessment due to lack of an endpoint attributable to a single exposure scenario for dimethipin, the oral LD50 of 458 mg/ kg is used to assess acute risk. A NOAEL of 200 ppm a. i. was obtained in a 2 generation reproductive study in rats ( MRID 9308903), for use in chronic risk assessment.  61 Table E 3. Mammalian toxicity ( from HED).

Species % a. i. Test Type Toxicity Value and Affected Endpoints Identification Number Study Classification Rat Not reported by HED Acute oral toxicity LD50 = 458 mg/ kg (

)

LD50 = 546 mg/ kg (  ) MRID 42429601 No classification made by HED Rat 99.7 2 Generation Reproductive study Dietary exposure to 0, 50, 200, or 800 ppm Parental systemic NOAEL for

= 200 ppm for decreased body weight and body weight gain ( equivalent to 11.8 29.0 mg/ kg/ day); NOAEL for  > 800 ppm ( equivalent to 35.5 115.7 mg/ kg/ day)

Reproductive toxicity NOAEL for

and  > 800 ppm ( equivalent to 35.5 115.7 mg/ kg/ day in

and 31.2 12. 3 mg/ kg/ day in  )

Offspring toxicity NOAEL for

and  = 200 ppm for decreased body weights and/ or decreased body weight gain by the F1a, F2a, and F2b pups ( equivalent to11.8 92.0 mg/ kg/ day in

and 9.2 28.7 mg/ kg/ day in  ) MRID 93089034 acceptable/

guideline  62 d. Terrestrial Insects: Acute A honey bee acute contact study using the TGAI is required for dimethipin because its outdoor treatment use will result in honey bee exposure. The acute contact LD50, using the honey bee, Apis mellifera, is an acute contact, single dose laboratory study designed to estimate the quantity of toxicant required to cause 50% mortality in a test population of bees. The TGAI is administered by one of two methods: whole body exposure to technical pesticide in a nontoxic dust diluent; or, topical exposure to technical pesticide via micro applicator. The median lethal dose ( LD50) is expressed in micrograms of active ingredient per bee ( µ g a. i./ bee). The following toxicity category descriptions have been used to characterize honey bee acute contact toxicity values:

LD50 ( µ g a. i./ bee) Toxicity Category < 2 Highly toxic 2   < 11 Moderately toxic > 11 Practically nontoxic Results of the acute exposure studies in honey bees are tabulated below ( Table E 4). The acute contact study for the dimethipin formulation is considered to be a core study because, despite the fact that the TGAI was not tested, dimethipin was shown to have a low toxicity to bees. Based on the reported toxicity values, it is not clear if the LD50 value is expressed in terms of µ g a. i./ bee or µ g formulation/ bee. The Harvade 25F formulation contains 22.4% a. i. Taking a conservative approach by assuming that the LD50 value is expressed in terms of the formulation, the LD50 value expressed in terms of TGAI would be 22.4 µ g a. i./ bee ( 100 µ g formulation/ bee × 22.4% a. i./ formulation = 22.4 µ g a. i./ bee). Thus, dimethipin is classified as practically non toxic to bees on a contact exposure basis. Data submitted under Guideline 141 1, Honey Bee Acute Contact, are considered useful for purposes of risk assessment ( MRID 41264606).

The acute oral toxicity study ( MRID 41264606) is categorized as supplemental because the submission of honey bee acute oral toxicity studies is not a guideline requirement and the core classification is reserved for guideline studies that fulfill guideline requirements.  63 Table E 4 Non target insect acute contact toxicity.

Species % a. i. Toxicity Value and Category Identification Number Study Classification1 Honey bee ( Apis mellifera) Harvade 25F   purity not reported Acute contact study;

48 hour LC50 > 100 µ g/ bee2; practically nontoxic MRID 41264606 ( Cole 1985) Core3 Honey bee ( Apis mellifera) Harvade 25F   purity not reported Acute oral study;

48 hour LC50 > 100 µ g/ bee2; not rated4 MRID 41264606 ( Cole 1985) Supplemental3 1Core: study satisfies guideline; Supplemental: study is scientifically sound, but does not satisfy guideline; Invalid: study is not scientifically sound and does not satisfy guideline.

2Not specified in DER if this value is in terms of µ g a. i./ bee or µ g formulation/ bee.

3Both the acute contact and acute oral studies were reported in the same study ( MRID 4126460). Although both portions of this study are considered scientifically sound, only the acute contact study is classified as " core", because acute oral toxicity studies in honey bees are not a guideline requirement.

4OPP does not categorize honey bee acute oral toxicity.

3. Toxicity to Freshwater Aquatic Animals a. Freshwater Fish: Acute In general, categories of acute toxicity for aquatic organisms can be classified according to the toxicity reference value ( LC50) given by a study ( EPA 2001):

LC50 ( ppm) Toxicity Category < 0.1 Very highly toxic 0.1   1 Highly toxic > 1   10 Moderately toxic > 10   100 Slightly toxic  64 > 100 Practically nontoxic Two freshwater fish toxicity studies using the TGAI are required to establish the acute toxicity of dimethipin to fish. The preferred test species are bluegill sunfish ( a warmwater fish) and rainbow trout ( a coldwater fish). The data that were submitted show that the LC50 is 20.9 ppm for bluegill sunfish ( MRID 41945902) and 52.8 ppm for rainbow trout ( MRID 41945903) ( Table E 5). Based on these results, dimethipin is categorized as slightly toxic to freshwater fish on an acute basis. EFED will use the worstcase value, LC50 of 20.9 ppm, for evaluating acute risks to freshwater fish. Data submitted under Guideline (# 72 1) are considered useful for purposes of risk assessment ( MRID 41945902; MRID 41945903).

Table E 5 Freshwater fish acute toxicity.

Species % a. i. 96 Hour LC50 ( mg a. i./ L) Toxicity Category Identification Number Study Classification1 Bluegill sunfish ( Lopomis macrochirus) 98.18 ( Harvade technical) 20.9 NOAEC ( for mortality and signs of toxicity): 6.3 Slightly toxic MRID 41945902 ( Ward 1991) Core Rainbow trout ( Oncorhynchus mykiss) 98.18 ( Harvade technical) 52.8 NOAEC ( for mortality and signs of toxicity:) 10.3 Slightly toxic MRID 41945903 ( Ward 1991) Core 1Core: study satisfies guideline; Supplemental: study is scientifically sound, but does not satisfy guideline; Invalid: study is not scientifically sound and does not satisfy guideline.

b. Freshwater Fish: Chronic A freshwater fish early life stage test using the TGAI is required for dimethipin because the end use product may be transported to water from the intended use site, and the following conditions are met: ( 1) dimethipin is intended for use such that its presence in water is likely to be continuous or recurrent  65 regardless of toxicity; ( 2) studies on aquatic invertebrates showed reproductive effects ( daphnid 21 day LOAEC = 1.0 ppm); and/ or ( 3) dimethipin is persistent in water. As summarized in Table E 6 results of an early life stage study in fathead minnow yield NOAEC and LOAEC values for larval survival of 12 ppm and 24 ppm, respectively ( MRID 00126069). Although this study is scientifically sound, it is classified as supplemental due to several deviations from guidance, including the failure to report purity of the test substance. Thus, although data reported in this study are usable in this risk assessment, the Guideline ( 72 4a) has not been fulfilled.

Table E 6 Freshwater fish chronic toxicity.

Species % a. i. Toxicity Value Identification Number Study Classification1 Fathead minnow ( Pimephales promelas) Purity not reported ( Harvade Technical) Fish Early life Stage Study.

Embryos exposed for 31 days, from hatch to 31 days.

NOAEC ( for larval survival):

12 ppm LOAEC ( for larval survival):

24 ppm MRID 00126069 ( Wilson and Altshul, no date) Supplemental ( classified as supplemental due to several deviations from guidance, including purity of tecnical not reported)

1Core: study satisfies guideline; Supplemental: study is scientifically sound, but does not satisfy guideline; Invalid: study is not scientifically sound and does not satisfy guideline.

c. Freshwater Invertebrates: Acute A freshwater aquatic invertebrate toxicity test using the TGAI is required to establish the toxicity of dimethipin to aquatic invertebrates. The preferred test species is Daphnia magna. The data that were submitted show that dimethipin is slightly to moderately toxic to daphnids, with acute 48 hour LC50 values ranging from 6.6 to 28 ppm ( Table E 7). Although EFED typically uses the worst case value for evaluating acute toxic exposure to freshwater invertebrates, the lowest LC50/ EC50 of 6.6 ppm ( MRID 46063401) was reported in a study using the dimethipin formulation Harvade 25F ( purity 23.2% a. i.), not the TGAI. Thus, the EC50 value of 20 ppm a. i ( MRID not provided, LeBlanc 1977) is used for evaluating acute toxic exposure to freshwater invertebrates. The Guideline requirements ( 72 2) for acute invertebrate toxicity are fulfilled ( MRID not provided, LeBlanc, 1977; MRID 00126070; MRID 41945904).  66 Table E 7. Freshwater invertebrate acute toxicity.

Species % a. i. LC50 ( ppm a. i.) Toxicity Category Identification Number Study Classification1 Daphnia magna 99.2 ( Harvade Technical) 48 hour LC50: 20 NOAEC: 14 Slightly toxic 00086315 ( LeBlanc 1977) Core Daphnia magna 98.18 ( Harvade Technical) 96 hour LC50: 21.3 Slightly toxic MRID 41945904 ( Ward 1991) Core Daphnia magna 23.2 ( Harvade 25F) 48 hour LC50/ EC50: 6.6 NOAEC ( for mortality/ immobility): 3.16 LOAEC ( for mortality/ immobility): 9.82 Moderately toxic ( FP) MRID 46063401 ( Czech 2003) Supplemental ( classified as supplemental because not all treatments were analytically verified at 0 and 48 hours)

1Core: study satisfies guideline; Supplemental: study is scientifically sound, but does not satisfy guideline; Invalid: study is not scientifically sound and does not satisfy guideline.

d. Freshwater Invertebrate: Chronic A freshwater aquatic invertebrate life cycle test using the TGAI is required for dimethipin. The preferred test is a 21 day life cycle on Daphnia magna. The data, summarized in Table E 8, that were submitted show that dimethipin has the potential for chronic toxicity to daphnids and possibly other freshwater invertebrates. Exposure to 1.0 ppm a. i. resulted in growth effects and exposure to 2.1 ppm a. i. reduced reproduction ( young/ adult/ reproductive day). The NOAEC of 0.61 ppm a. i. ( length) is used in assessing risk. Risk quotients under evaluated uses are far below a Level of Concern. The Guideline ( 72 4) is fulfilled ( MRID 00128803). One chronic toxicity study in daphnids was classified as Invalid ( MRID 00126070).  67  68 Table E 8. Freshwater invertebrate chronic toxicity.

Species % a. i. Toxicity Value Identification Number Study Classification1 Daphnia magna 99.1 ( Harvade technical) 21 day life cycle test. Mortality, number of young/ adult/ reproductive day, and length ( growth) decreased by dimethipin exposure. Most sensitive effect was length.

Length:

NOAEC: 0.61 ppm a. i.

LOAEC: 1.0 ppm a. i.

Number of young/ reproduction day:

NOAEC: 1.0 ppm a. i.

LOAEC: 2.1 ppm a. i.

Mortality:

NOAEC: 3.3 ppm a. i.

LOAEC: 9.1 ppm a. i. MRID 00128803 ( Forbis et al. 1982) Core 1Core: study satisfies guideline; Supplemental: study is scientifically sound, but does not satisfy guideline; Invalid: study is not scientifically sound and does not satisfy guideline.

4. Toxicity to Estuarine/ Marine Animals a. Estuarine/ Marine Fish: Acute Acute toxicity testing with estuarine/ marine fish using the TGAI is required for dimethipin. The preferred test species is the sheepshead minnow ( Cyprinodon variegatus). The data submitted showed that the LC50 = 17.8 ppm ( Table E 9; therefore, dimethipin is categorized as slightly toxic to estuarine/ marine fish on an acute basis. The Guideline ( 72 3) is fulfilled ( MRID 41663901).  69 Table E 9. Estuarine/ marine fish acute toxicity.

Species % a. i. 96 hour LC50 ( ppm a. i.) Toxicity Category Identification Number Study Classification1 Sheepshead minnow ( Cypridodon variegatus) 91.18 ( Harvade technical) 96 hour LC50: 17.8 NOAEC ( for mortality): 11.0 Slightly toxic MRID 41663901 ( Ward 1990) Core 1Core: study satisfies guideline; Supplemental: study is scientifically sound, but does not satisfy guideline; Invalid: not is scientifically valid, does not satisfy guideline b. Estuarine/ Marine Fish: Chronic No data on the effects of chronic exposure of estuarine/ marine fish to dimethipin were submitted.

c. Estuarine/ Marine Aquatic Invertebrates: Acute Acute toxicity testing with estuarine/ marine invertebrates using the TGAI is required for dimethipin because the end use product is intended for direct application to the estuarine/ marine environment or the active ingredient is expected to reach this environment because of its use in coastal communities. The preferred test species are mysid shrimp and eastern oyster. Based on the results of the core studies summarized in Table E 10, dimethipin is categorized as slightly toxic to the eastern oyster ( 96 hour EC50 values of 12.4 ppm, MRID 41666501, and 18.6 ppm, MRID 41663902) and to mysid shrimp ( 48 hour LC50 of 13.9 ppm a. i., MRID 41663902). The Core LC50 value reported of 13.9 ppm a. i., from the mysid shrimp study ( MRID 41663902) will be used to characterize risk of acute dimethipin exposures to estuarine/ marine aquatic invertebrates, and for use in an acute/ chronic extrapolation based on freshwater invertebrate data. The data requirements ( 72 3b and 72 3c) are fulfilled ( MRID 42643101; MRID 41663902).  70 Table E 10. Estuarine/ marine invertebrate acute toxicity.

Species % a. i. LC50/ EC50 ( ppm a. i.) Toxicity Category Identification Number Study Classification1 Mysid shrimp ( Mysidopsis bahia) 91.18 ( Harvade technical) 48 hour LC50: 13.9 NOAEC ( for mortality) = 5.9 Slightly toxic MRID 41663902 ( Ward 1990) Core Eastern oyster ( Crassostrea virginica) 98.18 ( Harvade technical) 96 hour shell deposition study EC50 ( for shell growth) = 12.4 ppm NOAEC ( for shell growth) = 4.67 ppm Slightly toxic MRID 41666501 ( Ward 1990) Supplemental Eastern oyster ( Crassostrea virginica) 98.51 ( Harvade technical) 96 hour shell deposition study EC50 ( for shell deposition): 18.6 ppm a. i.

NOAEC ( for shell deposition) = 10.1 ppm a. i. Slightly toxic MRID 42643101 ( McElwee 1993) Core 1Core: study satisfies guideline; Supplemental: study is scientifically sound, but does not satisfy guideline; Invalid: study is not scientifically sound and does not satisfy guideline.

d. Estuarine/ Marine Invertebrate: Chronic An estuarine/ marine invertebrate life cycle toxicity test using the TGAI is required for dimethipin because the end use product is expected to transport to an estuarine/ marine environment from the intended use site, and the following conditions are met: ( 1) the pesticide is intended for use such that its presence in water is likely to be continuous or recurrent regardless of toxicity; and ( 2) studies of other organisms  71 indicate that the reproductive physiology of fish and/ or invertebrates may be affected, physicochemical properties indicate cumulative effects, or the pesticide is persistent in water. The preferred test species is mysid shrimp. No data were submitted. The Guideline ( 72 4c) has not been fulfilled.

5. Toxicity to Aquatic and Terrestrial Plants a. Aquatic Plants Several aquatic plant toxicity studies using the TGAI are required to establish the toxicity of dimethipin to non target aquatic plants. The recommendation is for testing on five species: freshwater green alga ( Selenastrum capricornutum), duckweed ( Lemna gibba), marine diatom ( Skeletonema costatum), bluegreen algae ( Anabaena flos aquae), and a freshwater diatom. Studies submitted for each of the five recommended species, summarized in Table E 11, showed that exposure to dimethipin at levels greater than 2.5 ppm reduced growth of aquatic non vascular plants and levels greater than 0.8 ppm decreased the frond count in aquatic vascular plants. EFED will use the worst case EC50 value, 2.1 ppm ( vascular plants), for evaluating acute toxic exposure to non target aquatic plants. The Guideline requirements ( 123 2) are fulfilled ( MRID 42627104; MRID 41264605; MRID 42627103; MRID 42627101; MRID 42627102) for all five required species.

Table E 11. Aquatic plant acute toxicity.

Species % a. i. EC50 ( ppm a. i.) Identification Number Study Classification1 Duckweed ( Lemna gibba) 98.51 ( Harvade technical) Tier 2 aquatic plant test.

Endpoint: frond count 14 day EC50 ( for decreased growth): 2.1 NOAEC: 0.8 LOAEC: 1.3 MRID 42627104 ( McElwee 1993) Core Table E 11. Aquatic plant acute toxicity.

Species % a. i. EC50 ( ppm a. i.) Identification Number Study Classification1  72 Green algae ( Selenastrum capricornutum) Purity not reported ( dimethipin technical) Tier 2 aquatic plant test.

Endpoint: growth ( not specified)

5 day EC50 = 5.12 NOAEC: 2.5 MRID 41264605 ( Richards and Kaiser 1982) Core Saltwater diatom ( Skeletonema costatum) 98.51 ( Harvade technical) Tier 2 aquatic plant test. Endpoint: cell count 5 day EC50: 12.2 NOAEC: 7.3 LOAEC: 12.6 MRID 42627103 ( McElwee 1993) Core Freshwater diatom ( Nitzschia palea) 98.51 ( Harvade technical) Tier 2 aquatic plant test.

Endpoint: cell count 5 day EC50: 17.6 NOAEC: 11.3 LOAEC: 18.9 MRID 42627102 ( McElwee 1993) Core Blue green algae ( Anabaena flosaquae 98.51 ( Harvade technical) Tier 2 aquatic plant test.

Endpoint: cell count 5 day EC50: 99.5 NOAEC: 13.6 LOAEC: 22.4 MRID 42627101 ( McElwee 1993) Core Table E 11. Aquatic plant acute toxicity.

Species % a. i. EC50 ( ppm a. i.) Identification Number Study Classification1  73 1Core: study satisfies guideline; Supplemental: study is scientifically sound, but does not satisfy guideline; Invalid: study is not scientifically sound and does not satisfy guideline.

b. Terrestrial Plants Terrestrial Tier II studies are required since dimethipin is a defoliant/ herbicide. The recommendations for seedling emergence and vegetative vigor studies are for testing of: ( 1) six species of at least four dicotyledonous families, one species of which is soybean ( Glycine max) and the second of which is a root crop; and ( 2) four species of at least two monocotyledonous families, one of which is corn ( Zea mays). All studies submitted to fulfill Guidelines 123 1( a) and 123 1( b) are currently considered invalid for risk assessment. The available data are not sufficient to use to quantify the risks of dimethipin exposure to non target plants. The Guidelines 123 1a and 123 1b are not fulfilled.  74 Appendix F. The Risk Quotient Method and Levels of Concern The risks to terrestrial and aquatic organisms are determined based on a method by which risk quotients ( RQs) are compared with levels of concern ( LOCs). This method provides an indication of a chemical's potential to cause an effect in the field from effects observed in laboratory studies, when used as directed. Risk quotients are expressed as the ratio of the estimated environmental concentration ( EEC) to the speciesspecific toxicity reference value ( TRV):

RQ EEC TRV =

Units for EEC and TRV should be the same ( e. g., µ g/ L or ppb). The RQ is compared to the LOC as part of a risk characterization. Acute and chronic LOCs for terrestrial and aquatic organisms are given in recent Agency guidance ( EPA 2004a) and summarized in Table F 1 below.  75 Table F 1. Level of concern ( LOC) by risk presumption category ( EPA 2004).

Risk Presumption RQ LOC Mammals and Birds Acute Risk1 EEC2/ LC50 or LD50/ sqft3 or LD50/ day4 0.5 Acute Restricted Use5 EEC/ LC50 or LD50/ sqft or LD50/ day ( or LD50 < 50 mg/ kg) 0.2 Acute Endangered Species6 EEC/ LC50 or LD50/ sqft or LD50/ day 0.1 Chronic Risk EEC/ NOEC 1 Aquatic Animals Acute Risk EEC7/ LC50 or EC50 0.5 Acute Restricted Use EEC/ LC50 or EC50 0.1 Acute Endangered Species EEC/ LC50 or EC50 0.05 Chronic Risk EEC/ NOEC 1 Terrestrial and Semi Aquatic Plants Acute Risk EEC/ EC25 1 Acute Endangered Species EEC/ EC05 or NOEC 1 Aquatic Plants Acute Risk EEC8/ EC50 1 Acute Endangered Species EEC7/ EC05 or NOEC 1 1Potential for acute risk to non target organisms which may warrat regulatory action in addition to restricted use classification ( EPA 2004).

2Estimated environmental concentration ( ppm) on avian/ mammalian food items 3mg/ ft2 4mg of toxicant consumed per day 5Potential for acute risk to non target organisms, but may be mitigated through restricted use classification ( EPA 2004).

6Endangered species may be potentially affected by use ( EPA 2004).

7EEC = ppb or ppm in water 8EEC = lbs ai/ A  76 For acute exposure to terrestrial and aquatic plants, an LOC of 1 is used. Currently the Agency does not perform assessments for chronic risk to plants or acute/ chronic risks to non target insects.

For this Tier I assessment of dimethipin, acute exposure to aquatic organisms is represented by the maximum 24 hour EEC value calculated using GENEEC2. EECs used to assess acute and chronic risk to avian and mammalian species were calculated using ELL FATE.  77 Appendix G. Summary of Endangered/ Threatened Species Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Alabama County County Status Presence Acres Autauga Mammal BAT, INDIANA Myotis sodalis Endangered possible 9956 Crop Acres ( inclusive of species crop overlaps): 9956 Baldwin Mammal MOUSE, ALABAMA BEACH Peromyscus polionotus ammobates Endangered known 13040 MOUSE, PERDIDO KEY BEACH Peromyscus polionotus trissyllepsis Endangered known 13040 Crop Acres ( inclusive of species crop overlaps): 26080 Colbert Mammal BAT, GRAY Myotis grisescens Endangered known 25509 BAT, INDIANA Myotis sodalis Endangered possible 25509 Crop Acres ( inclusive of species crop overlaps): 51018 Conecuh Mammal  78 BAT, GRAY Myotis grisescens Endangered known 1260 Crop Acres ( inclusive of species crop overlaps): 1260 Lauderdale Mammal BAT, GRAY Myotis grisescens Endangered known 22968 Crop Acres ( inclusive of species crop overlaps): 22968 Lawrence Mammal BAT, GRAY Myotis grisescens Endangered known 24769 Monday, October 04, 2004 Page 1 of 27  79 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Alabama County County Status Presence Acres Lawrence BAT, INDIANA Myotis sodalis Endangered possible 24769 Crop Acres ( inclusive of species crop overlaps): 49538 Limestone Mammal BAT, GRAY Myotis grisescens Endangered known 21951 BAT, INDIANA Myotis sodalis Endangered possible 21951 Crop Acres ( inclusive of species crop overlaps): 43902 Madison Mammal BAT, GRAY Myotis grisescens Endangered known 5456 Crop Acres ( inclusive of species crop overlaps): 5456 Morgan Mammal BAT, GRAY Myotis grisescens Endangered known 2110 BAT, INDIANA Myotis sodalis Endangered known 2110 Crop Acres ( inclusive of species crop overlaps): 4220 Shelby Mammal  80 BAT, GRAY Myotis grisescens Endangered known 4313 BAT, INDIANA Myotis sodalis Endangered known 4313 Crop Acres ( inclusive of species crop overlaps): 8626 Arizona County County Status Presence Acres Cochise Monday, October 04, 2004 Page 2 of 27  81 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Arizona County County Status Presence Acres Cochise Mammal BAT, LESSER (= SANBORN'S) Leptonycteris curasoae yerbabuenae Endangered known 6731 LONG NOSED JAGUAR Panthera onca Endangered known 6731 Jaguarundi, Sinaloan Herpailurus (= Felis) yagouaroundi tolteca Endangered 6731 OCELOT Leopardus (= Felis) pardalis Endangered possible 6731 WOLF, GRAY Canis lupus Threatened possible 6731 Crop Acres ( inclusive of species crop overlaps): 33655 Graham Mammal BAT, LESSER (= SANBORN'S) Leptonycteris curasoae yerbabuenae Endangered known 22903 LONG NOSED JAGUAR Panthera onca Endangered known 22903 Jaguarundi, Sinaloan Herpailurus (= Felis) yagouaroundi tolteca Endangered 22903 OCELOT Leopardus (= Felis) pardalis Endangered possible 22903 SQUIRREL, MOUNT GRAHAM RED Tamiasciurus hudsonicus grahamensis Endangered known 22903 Crop Acres ( inclusive of species crop overlaps): 114515 Greenlee Mammal JAGUAR Panthera onca Endangered known 1004  82 Crop Acres ( inclusive of species crop overlaps): 1004 Maricopa Mammal BAT, LESSER (= SANBORN'S) Leptonycteris curasoae yerbabuenae Endangered known 112706 LONG NOSED PRONGHORN, SONORAN Antilocapra americana sonoriensis Endangered known 112706 Crop Acres ( inclusive of species crop overlaps): 225412 Monday, October 04, 2004 Page 3 of 27  83 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Arizona County County Status Presence Acres Mohave Mammal VOLE, HUALAPAI MEXICAN Microtus mexicanus hualpaiensis Endangered known 3977 Crop Acres ( inclusive of species crop overlaps): 3977 Pima Mammal BAT, LESSER (= SANBORN'S) Leptonycteris curasoae yerbabuenae Endangered known 14570 LONG NOSED JAGUAR Panthera onca Endangered known 14570 PRONGHORN, SONORAN Antilocapra americana sonoriensis Endangered known 14570 Crop Acres ( inclusive of species crop overlaps): 43710 Pinal Mammal BAT, LESSER (= SANBORN'S) Leptonycteris curasoae yerbabuenae Endangered known 118608 LONG NOSED JAGUAR Panthera onca Endangered known 118608 Crop Acres ( inclusive of species crop overlaps): 237216 Yuma Mammal BAT, LESSER (= SANBORN'S) Leptonycteris curasoae yerbabuenae Endangered known 27972 LONG NOSED  84 PRONGHORN, SONORAN Antilocapra americana sonoriensis Endangered known 27972 Crop Acres ( inclusive of species crop overlaps): 55944 California County County Status Presence Acres Fresno Mammal Monday, October 04, 2004 Page 4 of 27  85 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres California County County Status Presence Acres Fresno FOX, SAN JOAQUIN KIT Vulpes macrotis mutica Endangered known 348003 KANGAROO RAT, FRESNO Dipodomys nitratoides exilis Endangered known 348003 KANGAROO RAT, GIANT Dipodomys ingens Endangered known 348003 Crop Acres ( inclusive of species crop overlaps): 1044009 Kern Mammal FOX, SAN JOAQUIN KIT Vulpes macrotis mutica Endangered known 265462 KANGAROO RAT, GIANT Dipodomys ingens Endangered known 265462 KANGAROO RAT, TIPTON Dipodomys nitratoides nitratoides Endangered known 265462 SHREW, BUENA VISTA Sorex ornatus relictus Endangered known 265462 Crop Acres ( inclusive of species crop overlaps): 1061848 Kings Mammal FOX, SAN JOAQUIN KIT Vulpes macrotis mutica Endangered known 196108 KANGAROO RAT, FRESNO Dipodomys nitratoides exilis Endangered possible 196108 KANGAROO RAT, GIANT Dipodomys ingens Endangered known 196108 KANGAROO RAT, TIPTON Dipodomys nitratoides nitratoides Endangered known 196108 Crop Acres ( inclusive of species crop overlaps): 784432 Madera  86 Mammal FOX, SAN JOAQUIN KIT Vulpes macrotis mutica Endangered known 36806 KANGAROO RAT, FRESNO Dipodomys nitratoides exilis Endangered possible 36806 Crop Acres ( inclusive of species crop overlaps): 73612 Merced Mammal Monday, October 04, 2004 Page 5 of 27  87 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres California County County Status Presence Acres Merced FOX, SAN JOAQUIN KIT Vulpes macrotis mutica Endangered known 74620 KANGAROO RAT, FRESNO Dipodomys nitratoides exilis Endangered possible 74620 KANGAROO RAT, GIANT Dipodomys ingens Endangered possible 74620 Crop Acres ( inclusive of species crop overlaps): 223860 Riverside Mammal KANGAROO RAT, SAN BERNARDINO Dipodomys merriami parvus Endangered known 12571 KANGAROO RAT, STEPHENS' Dipodomys stephensi ( incl. D. cascus) Threatened known 12571 SHEEP, PENINSULAR BIGHORN Ovis canadensis Threatened known 12571 Crop Acres ( inclusive of species crop overlaps): 37713 San Bernardino Mammal KANGAROO RAT, SAN BERNARDINO Dipodomys merriami parvus Endangered known 339 KANGAROO RAT, STEPHENS' Dipodomys stephensi ( incl. D. cascus) Threatened possible 339 VOLE, AMARGOSA Microtus californicus scirpensis Endangered known 339 Crop Acres ( inclusive of species crop overlaps): 1017 Tulare Mammal  88 FOX, SAN JOAQUIN KIT Vulpes macrotis mutica Endangered known 81089 KANGAROO RAT, GIANT Dipodomys ingens Endangered known 81089 KANGAROO RAT, TIPTON Dipodomys nitratoides nitratoides Endangered known 81089 Crop Acres ( inclusive of species crop overlaps): 243267 Florida County County Status Presence Acres Monday, October 04, 2004 Page 6 of 27  89 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Florida County County Status Presence Acres Escambia Mammal MOUSE, PERDIDO KEY BEACH Peromyscus polionotus trissyllepsis Endangered known 12422 Crop Acres ( inclusive of species crop overlaps): 12422 FLORIDA Mammal WHALE, NORTHERN RIGHT Eubalaena glacialis Endangered known 93504 Crop Acres ( inclusive of species crop overlaps): 93504 Jackson Mammal BAT, GRAY Myotis grisescens Endangered known 23273 BAT, INDIANA Myotis sodalis Endangered possible 23273 Crop Acres ( inclusive of species crop overlaps): 46546 Walton Mammal MOUSE, CHOCTAWHATCHEE BEACH Peromyscus polionotus allophrys Endangered known 3711 Crop Acres ( inclusive of species crop overlaps): 3711 Georgia County  90 County Status Presence Acres Bartow Mammal BAT, GRAY Myotis grisescens Endangered possible 2556 BAT, INDIANA Myotis sodalis Endangered possible 2556 Crop Acres ( inclusive of species crop overlaps): 5112 Monday, October 04, 2004 Page 7 of 27  91 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Georgia County County Status Presence Acres Calhoun Mammal BAT, INDIANA Myotis sodalis Endangered possible 17659 Crop Acres ( inclusive of species crop overlaps): 17659 Clay Mammal BAT, INDIANA Myotis sodalis Endangered possible 4753 Crop Acres ( inclusive of species crop overlaps): 4753 Crawford Mammal BAT, INDIANA Myotis sodalis Endangered possible 700 Crop Acres ( inclusive of species crop overlaps): 700 Early Mammal BAT, INDIANA Myotis sodalis Endangered possible 33630 Crop Acres ( inclusive of species crop overlaps): 33630 Floyd Mammal BAT, GRAY Myotis grisescens Endangered possible 3769  92 BAT, INDIANA Myotis sodalis Endangered possible 3769 Crop Acres ( inclusive of species crop overlaps): 7538 Henry Mammal BAT, INDIANA Myotis sodalis Endangered possible 792 Crop Acres ( inclusive of species crop overlaps): 792 Monday, October 04, 2004 Page 8 of 27  93 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Georgia County County Status Presence Acres Lamar Mammal BAT, INDIANA Myotis sodalis Endangered possible 2777 Crop Acres ( inclusive of species crop overlaps): 2777 Marion Mammal BAT, INDIANA Myotis sodalis Endangered possible 1399 MANATEE, WEST INDIAN ( FLORIDA) Trichechus manatus Endangered known 1399 Crop Acres ( inclusive of species crop overlaps): 2798 Miller Mammal BAT, INDIANA Myotis sodalis Endangered possible 23943 Crop Acres ( inclusive of species crop overlaps): 23943 Morgan Mammal BAT, INDIANA Myotis sodalis Endangered possible 734 Crop Acres ( inclusive of species crop overlaps): 734 Pike Mammal  94 BAT, INDIANA Myotis sodalis Endangered possible 293 Crop Acres ( inclusive of species crop overlaps): 293 Polk Mammal BAT, GRAY Myotis grisescens Endangered possible 1876 BAT, INDIANA Myotis sodalis Endangered possible 1876 Monday, October 04, 2004 Page 9 of 27  95 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Georgia County County Status Presence Acres Polk Crop Acres ( inclusive of species crop overlaps): 3752 Quitman Mammal BAT, INDIANA Myotis sodalis Endangered possible 2795 Crop Acres ( inclusive of species crop overlaps): 2795 Randolph Mammal BAT, INDIANA Myotis sodalis Endangered possible 9129 Crop Acres ( inclusive of species crop overlaps): 9129 Schley Mammal BAT, INDIANA Myotis sodalis Endangered possible 1154 Crop Acres ( inclusive of species crop overlaps): 1154 Seminole Mammal BAT, INDIANA Myotis sodalis Endangered possible 23038 Crop Acres ( inclusive of species crop overlaps): 23038 Stewart  96 Mammal BAT, INDIANA Myotis sodalis Endangered possible 2723 Crop Acres ( inclusive of species crop overlaps): 2723 Terrell Mammal BAT, INDIANA Myotis sodalis Endangered possible 16493 Monday, October 04, 2004 Page 10 of 27  97 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Georgia County County Status Presence Acres Terrell Crop Acres ( inclusive of species crop overlaps): 16493 Webster Mammal BAT, INDIANA Myotis sodalis Endangered possible 4718 Crop Acres ( inclusive of species crop overlaps): 4718 Louisiana County County Status Presence Acres Catahoula Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 45984 Crop Acres ( inclusive of species crop overlaps): 45984 Concordia Mammal BEAR, AMERICAN BLACK Ursus americanus Threatened known 43443 BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 43443 Crop Acres ( inclusive of species crop overlaps): 86886 Franklin  98 Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 87336 Crop Acres ( inclusive of species crop overlaps): 87336 Grant Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 2973 Crop Acres ( inclusive of species crop overlaps): 2973 Monday, October 04, 2004 Page 11 of 27  99 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Louisiana County County Status Presence Acres Madison Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 40138 Crop Acres ( inclusive of species crop overlaps): 40138 Pointe Coupee Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 3997 Crop Acres ( inclusive of species crop overlaps): 3997 Richland Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 89685 Crop Acres ( inclusive of species crop overlaps): 89685 St. Landry Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 1484 Crop Acres ( inclusive of species crop overlaps): 1484 Tensas Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 83009  100 Crop Acres ( inclusive of species crop overlaps): 83009 Missouri County County Status Presence Acres Dade Mammal BAT, GRAY Myotis grisescens Endangered known 667 Monday, October 04, 2004 Page 12 of 27  101 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Missouri County County Status Presence Acres Dade Crop Acres ( inclusive of species crop overlaps): 667 Mississippi County County Status Presence Acres Adams Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 2282 Crop Acres ( inclusive of species crop overlaps): 2282 Attala Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 9588 Crop Acres ( inclusive of species crop overlaps): 9588 Claiborne Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 2045 Crop Acres ( inclusive of species crop overlaps): 2045 Copiah Mammal  102 BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 2044 Crop Acres ( inclusive of species crop overlaps): 2044 Franklin Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 680 Crop Acres ( inclusive of species crop overlaps): 680 George Monday, October 04, 2004 Page 13 of 27  103 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Mississippi County County Status Presence Acres George Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 1136 Crop Acres ( inclusive of species crop overlaps): 1136 Greene Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 780 Crop Acres ( inclusive of species crop overlaps): 780 Hinds Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 13111 Crop Acres ( inclusive of species crop overlaps): 13111 Holmes Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 34930 Crop Acres ( inclusive of species crop overlaps): 34930 Humphreys Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 55206  104 Crop Acres ( inclusive of species crop overlaps): 55206 Issaquena Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 18681 Crop Acres ( inclusive of species crop overlaps): 18681 Jackson Monday, October 04, 2004 Page 14 of 27  105 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Mississippi County County Status Presence Acres Jackson Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 2216 Crop Acres ( inclusive of species crop overlaps): 2216 Jefferson Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 3219 Crop Acres ( inclusive of species crop overlaps): 3219 Leake Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 1863 Crop Acres ( inclusive of species crop overlaps): 1863 Lowndes Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 5151 Crop Acres ( inclusive of species crop overlaps): 5151 Madison Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 27237  106 Crop Acres ( inclusive of species crop overlaps): 27237 Rankin Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 4337 Crop Acres ( inclusive of species crop overlaps): 4337 Scott Monday, October 04, 2004 Page 15 of 27  107 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Mississippi County County Status Presence Acres Scott Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 1622 Crop Acres ( inclusive of species crop overlaps): 1622 Sharkey Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 34603 Crop Acres ( inclusive of species crop overlaps): 34603 Tunica Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 38672 Crop Acres ( inclusive of species crop overlaps): 38672 Warren Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 10649 Crop Acres ( inclusive of species crop overlaps): 10649 Washington Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 89884  108 Crop Acres ( inclusive of species crop overlaps): 89884 Yazoo Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 64441 Crop Acres ( inclusive of species crop overlaps): 64441 Monday, October 04, 2004 Page 16 of 27  109 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres North Carolina County County Status Presence Acres Beaufort Mammal WOLF, RED Canis rufus Endangered known 4602 Crop Acres ( inclusive of species crop overlaps): 4602 Bertie Mammal WOLF, RED Canis rufus Endangered known 34319 Crop Acres ( inclusive of species crop overlaps): 34319 Cleveland Mammal BAT, INDIANA Myotis sodalis Endangered possible 2875 Crop Acres ( inclusive of species crop overlaps): 2875 Currituck Mammal WOLF, RED Canis rufus Endangered known 1780 Crop Acres ( inclusive of species crop overlaps): 1780 Harnett Mammal WOLF, RED Canis rufus Endangered known 10978  110 Crop Acres ( inclusive of species crop overlaps): 10978 Hyde Mammal WOLF, RED Canis rufus Endangered known 4212 Crop Acres ( inclusive of species crop overlaps): 4212 Martin Monday, October 04, 2004 Page 17 of 27  111 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres North Carolina County County Status Presence Acres Martin Mammal SQUIRREL, CAROLINA NORTHERN Glaucomys sabrinus coloratus Endangered possible 37139 FLYING WOLF, RED Canis rufus Endangered known 37139 Crop Acres ( inclusive of species crop overlaps): 74278 NORTH CAROLINA Mammal WHALE, NORTHERN RIGHT Eubalaena glacialis Endangered known 677541 Crop Acres ( inclusive of species crop overlaps): 677541 Rutherford Mammal BAT, INDIANA Myotis sodalis Endangered known 341 Crop Acres ( inclusive of species crop overlaps): 341 Tyrrell Mammal WOLF, RED Canis rufus Endangered known 1311 Crop Acres ( inclusive of species crop overlaps): 1311 Washington  112 Mammal WOLF, RED Canis rufus Endangered known 7692 Crop Acres ( inclusive of species crop overlaps): 7692 New Mexico County County Status Presence Acres Chaves Monday, October 04, 2004 Page 18 of 27  113 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres New Mexico County County Status Presence Acres Chaves Mammal FERRET, BLACK FOOTED Mustela nigripes Endangered possible 6954 Crop Acres ( inclusive of species crop overlaps): 6954 Curry Mammal FERRET, BLACK FOOTED Mustela nigripes Endangered possible 2037 Crop Acres ( inclusive of species crop overlaps): 2037 Dona Ana Mammal FERRET, BLACK FOOTED Mustela nigripes Endangered possible 22016 Crop Acres ( inclusive of species crop overlaps): 22016 Eddy Mammal FERRET, BLACK FOOTED Mustela nigripes Endangered possible 9808 Crop Acres ( inclusive of species crop overlaps): 9808 Hidalgo Mammal BAT, LESSER (= SANBORN'S) Leptonycteris curasoae yerbabuenae Endangered known 1219 LONG NOSED  114 BAT, MEXICAN LONG NOSED Leptonycteris nivalis Endangered known 1219 FERRET, BLACK FOOTED Mustela nigripes Endangered possible 1219 JAGUAR Panthera onca Endangered known 1219 WOLF, GRAY Canis lupus Threatened known 1219 Crop Acres ( inclusive of species crop overlaps): 6095 Lea Monday, October 04, 2004 Page 19 of 27  115 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres New Mexico County County Status Presence Acres Lea Mammal FERRET, BLACK FOOTED Mustela nigripes Endangered possible 9531 Crop Acres ( inclusive of species crop overlaps): 9531 Luna Mammal FERRET, BLACK FOOTED Mustela nigripes Endangered known 6448 WOLF, GRAY Canis lupus Threatened possible 6448 Crop Acres ( inclusive of species crop overlaps): 12896 Quay Mammal FERRET, BLACK FOOTED Mustela nigripes Endangered possible 2389 Crop Acres ( inclusive of species crop overlaps): 2389 Roosevelt Mammal FERRET, BLACK FOOTED Mustela nigripes Endangered possible 7009 Crop Acres ( inclusive of species crop overlaps): 7009 Oklahoma County County Status Presence Acres  116 Wagoner Mammal BAT, INDIANA Myotis sodalis Endangered possible 739 Crop Acres ( inclusive of species crop overlaps): 739 Monday, October 04, 2004 Page 20 of 27  117 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres South Carolina County County Status Presence Acres SOUTH CAROLINA Mammal WHALE, NORTHERN RIGHT Eubalaena glacialis Endangered known 285858 Crop Acres ( inclusive of species crop overlaps): 285858 Tennessee County County Status Presence Acres Carroll Mammal BAT, INDIANA Myotis sodalis Endangered possible 9928 Crop Acres ( inclusive of species crop overlaps): 9928 Chester Mammal BAT, INDIANA Myotis sodalis Endangered possible 2654 Crop Acres ( inclusive of species crop overlaps): 2654 Crockett Mammal BAT, INDIANA Myotis sodalis Endangered possible 93514 Crop Acres ( inclusive of species crop overlaps): 93514  118 Dyer Mammal BAT, INDIANA Myotis sodalis Endangered possible 30026 Crop Acres ( inclusive of species crop overlaps): 30026 Fayette Mammal BAT, INDIANA Myotis sodalis Endangered possible 36504 Monday, October 04, 2004 Page 21 of 27  119 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Tennessee County County Status Presence Acres Fayette Crop Acres ( inclusive of species crop overlaps): 36504 Franklin Mammal BAT, GRAY Myotis grisescens Endangered known 3160 BAT, INDIANA Myotis sodalis Endangered possible 3160 Crop Acres ( inclusive of species crop overlaps): 6320 Gibson Mammal BAT, INDIANA Myotis sodalis Endangered possible 41313 Crop Acres ( inclusive of species crop overlaps): 41313 Hardeman Mammal BAT, INDIANA Myotis sodalis Endangered possible 11657 Crop Acres ( inclusive of species crop overlaps): 11657 Haywood Mammal BAT, INDIANA Myotis sodalis Endangered possible 105105 Crop Acres ( inclusive of species crop overlaps): 105105  120 Henderson Mammal BAT, INDIANA Myotis sodalis Endangered possible 1348 Crop Acres ( inclusive of species crop overlaps): 1348 Lake Mammal Monday, October 04, 2004 Page 22 of 27  121 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Tennessee County County Status Presence Acres Lake BAT, INDIANA Myotis sodalis Endangered possible 9402 Crop Acres ( inclusive of species crop overlaps): 9402 Lauderdale Mammal BAT, INDIANA Myotis sodalis Endangered possible 35383 Crop Acres ( inclusive of species crop overlaps): 35383 Lincoln Mammal BAT, GRAY Myotis grisescens Endangered known 3216 BAT, INDIANA Myotis sodalis Endangered possible 3216 Crop Acres ( inclusive of species crop overlaps): 6432 Madison Mammal BAT, INDIANA Myotis sodalis Endangered possible 29991 Crop Acres ( inclusive of species crop overlaps): 29991 Obion Mammal BAT, INDIANA Myotis sodalis Endangered possible 3130  122 Crop Acres ( inclusive of species crop overlaps): 3130 Rutherford Mammal BAT, GRAY Myotis grisescens Endangered known 816 BAT, INDIANA Myotis sodalis Endangered possible 816 Crop Acres ( inclusive of species crop overlaps): 1632 Monday, October 04, 2004 Page 23 of 27  123 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Tennessee County County Status Presence Acres Shelby Mammal BAT, INDIANA Myotis sodalis Endangered possible 9167 Crop Acres ( inclusive of species crop overlaps): 9167 Tipton Mammal BAT, INDIANA Myotis sodalis Endangered possible 43413 Crop Acres ( inclusive of species crop overlaps): 43413 Texas County County Status Presence Acres Anderson Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened possible 1345 Crop Acres ( inclusive of species crop overlaps): 1345 Atascosa Mammal OCELOT Leopardus (= Felis) pardalis Endangered known 220 Crop Acres ( inclusive of species crop overlaps): 220  124 Burleson Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 15376 Crop Acres ( inclusive of species crop overlaps): 15376 Cameron Mammal JAGUARUNDI, Gulf Coast Herpailurus (= Felis) yagouaroundi Endangered known 51952 Monday, October 04, 2004 Page 24 of 27  125 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Texas County County Status Presence Acres Cameron OCELOT Leopardus (= Felis) pardalis Endangered known 51952 Crop Acres ( inclusive of species crop overlaps): 103904 Hidalgo Mammal JAGUARUNDI, Gulf Coast Herpailurus (= Felis) yagouaroundi Endangered known 61890 OCELOT Leopardus (= Felis) pardalis Endangered known 61890 Crop Acres ( inclusive of species crop overlaps): 123780 Jim Wells Mammal JAGUARUNDI, Gulf Coast Herpailurus (= Felis) yagouaroundi Endangered known 7075 OCELOT Leopardus (= Felis) pardalis Endangered known 7075 Crop Acres ( inclusive of species crop overlaps): 14150 Kleberg Mammal JAGUARUNDI, Gulf Coast Herpailurus (= Felis) yagouaroundi Endangered known 38070 OCELOT Leopardus (= Felis) pardalis Endangered known 38070 Crop Acres ( inclusive of species crop overlaps): 76140 Live Oak  126 Mammal JAGUARUNDI, Gulf Coast Herpailurus (= Felis) yagouaroundi Endangered known 643 OCELOT Leopardus (= Felis) pardalis Endangered known 643 Crop Acres ( inclusive of species crop overlaps): 1286 Nueces Mammal JAGUARUNDI, Gulf Coast Herpailurus (= Felis) yagouaroundi Endangered known 100597 Monday, October 04, 2004 Page 25 of 27  127 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Texas County County Status Presence Acres Nueces OCELOT Leopardus (= Felis) pardalis Endangered known 100597 Crop Acres ( inclusive of species crop overlaps): 201194 Refugio Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened possible 21044 Crop Acres ( inclusive of species crop overlaps): 21044 Robertson Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened known 12082 Crop Acres ( inclusive of species crop overlaps): 12082 San Patricio Mammal JAGUARUNDI, Gulf Coast Herpailurus (= Felis) yagouaroundi Endangered known 81685 OCELOT Leopardus (= Felis) pardalis Endangered known 81685 Crop Acres ( inclusive of species crop overlaps): 163370 Starr Mammal JAGUARUNDI, Gulf Coast Herpailurus (= Felis) yagouaroundi Endangered known 4591  128 OCELOT Leopardus (= Felis) pardalis Endangered known 4591 Crop Acres ( inclusive of species crop overlaps): 9182 Victoria Mammal BEAR, LOUISIANA BLACK Ursus americanus luteolus Threatened possible 7956 Crop Acres ( inclusive of species crop overlaps): 7956 Monday, October 04, 2004 Page 26 of 27  129 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Texas County County Status Presence Acres Willacy Mammal JAGUARUNDI, Gulf Coast Herpailurus (= Felis) yagouaroundi Endangered known 86188 OCELOT Leopardus (= Felis) pardalis Endangered known 86188 Crop Acres ( inclusive of species crop overlaps): 172376 Virginia County County Status Presence Acres Accomack Mammal SQUIRREL, DELMARVA PENINSULA Sciurus niger cinereus Endangered known 796 FOX Crop Acres ( inclusive of species crop overlaps): 796 Northampton Mammal SQUIRREL, DELMARVA PENINSULA Sciurus niger cinereus Endangered known 2159 FOX Crop Acres ( inclusive of species crop overlaps): 2159 VIRGINIA Mammal  130 WHALE, NORTHERN RIGHT Eubalaena glacialis Endangered known 98244 Crop Acres ( inclusive of species crop overlaps): 98244 Monday, October 04, 2004 Page 27 of 27 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Alabama County County Status Presence Acres Autauga Plant PITCHER PLANT, ALABAMA Sarracenia rubra alabamensis Endangered known 9956 CANEBRAKE POTATO BEAN, PRICE'S Apios priceana Threatened known 9956 Crop Acres ( inclusive of species crop overlaps): 19912 Blount Plant SUNFLOWER, EGGERT'S Helianthus eggertii Threatened known 1394 Crop Acres ( inclusive of species crop overlaps): 1394  131 Bullock Plant TRILLIUM, RELICT Trillium reliquum Endangered known 1051 Crop Acres ( inclusive of species crop overlaps): 1051 Cherokee Plant BARBARA'S BUTTONS, MOHR'S Marshallia mohrii Endangered known 20041 HARPERELLA Ptilimnium nodosum Endangered known 20041 LEATHER FLOWER, ALABAMA Clematis socialis Endangered known 20041 PITCHER PLANT, GREEN Sarracenia oreophila Endangered known 20041 Crop Acres ( inclusive of species crop overlaps): 80164 Colbert Plant BLADDERPOD, LYRATE Lesquerella lyrata Threatened known 25509 Crop Acres ( inclusive of species crop overlaps): 25509 Elmore Monday, October 04, 2004 Page 1 of 31  132 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Alabama County County Status Presence Acres Elmore Plant PITCHER PLANT, ALABAMA Sarracenia rubra alabamensis Endangered known 19393 CANEBRAKE Crop Acres ( inclusive of species crop overlaps): 19393 Etowah Plant BARBARA'S BUTTONS, MOHR'S Marshallia mohrii Endangered known 3072 LEATHER FLOWER, ALABAMA Clematis socialis Endangered known 3072 PITCHER PLANT, GREEN Sarracenia oreophila Endangered known 3072 Crop Acres ( inclusive of species crop overlaps): 9216 Franklin Plant BLADDERPOD, LYRATE Lesquerella lyrata Threatened known 738 GRASS, TENNESSEE YELLOW EYED Xyris tennesseensis Endangered known 738 PRAIRIE CLOVER, LEAFY Dalea foliosa Endangered possible 738 Crop Acres ( inclusive of species crop overlaps): 2214 Henry Plant TRILLIUM, RELICT Trillium reliquum Endangered known 19632  133 Crop Acres ( inclusive of species crop overlaps): 19632 Lawrence Plant BLADDERPOD, LYRATE Lesquerella lyrata Threatened known 24769 PRAIRIE CLOVER, LEAFY Dalea foliosa Endangered known 24769 Crop Acres ( inclusive of species crop overlaps): 49538 Monday, October 04, 2004 Page 2 of 31  134 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Alabama County County Status Presence Acres Lee Plant HARPERELLA Ptilimnium nodosum Endangered known 2454 TRILLIUM, RELICT Trillium reliquum Endangered known 2454 Crop Acres ( inclusive of species crop overlaps): 4908 Madison Plant LEATHER FLOWER, MOREFIELD'S Clematis morefieldii Endangered known 5456 POTATO BEAN, PRICE'S Apios priceana Threatened known 5456 Crop Acres ( inclusive of species crop overlaps): 10912 Mobile Plant QUILLWORT, LOUISIANA Isoetes louisianensis Endangered possible 13313 Crop Acres ( inclusive of species crop overlaps): 13313 Morgan Plant FERN, AMERICAN HART'S TONGUE Asplenium scolopendrium var. Threatened known 2110 PRAIRIE CLOVER, LEAFY Dalea foliosa Endangered known 2110 Crop Acres ( inclusive of species crop overlaps): 4220  135 Arkansas County County Status Presence Acres Clay Plant PONDBERRY Lindera melissifolia Endangered known 28331 Crop Acres ( inclusive of species crop overlaps): 28331 Monday, October 04, 2004 Page 3 of 31  136 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Arkansas County County Status Presence Acres Drew Plant GEOCARPON MINIMUM Geocarpon minimum Endangered known 22714 Crop Acres ( inclusive of species crop overlaps): 22714 Jackson Plant PONDBERRY Lindera melissifolia Endangered known 1151 Crop Acres ( inclusive of species crop overlaps): 1151 Woodruff Plant PONDBERRY Lindera melissifolia Endangered known 6764 Crop Acres ( inclusive of species crop overlaps): 6764 Arizona County County Status Presence Acres Cochise Plant CACTUS, COCHISE PINCUSHION Coryphantha robbinsorum Threatened known 6731 UMBEL, HUACHUCA WATER Lilaeopsis schaffneriana var. recurva Endangered known 6731 Crop Acres ( inclusive of species crop overlaps): 13462  137 Graham Plant CLIFFROSE, ARIZONA Purshia (= cowania) subintegra Endangered known 22903 Crop Acres ( inclusive of species crop overlaps): 22903 Maricopa Plant Monday, October 04, 2004 Page 4 of 31  138 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Arizona County County Status Presence Acres Maricopa AGAVE, ARIZONA Agave arizonica Endangered known 112706 CACTUS, ARIZONA HEDGEHOG Echinocereus triglochidiatus var. Endangered known 112706 CLIFFROSE, ARIZONA Purshia (= cowania) subintegra Endangered known 112706 Crop Acres ( inclusive of species crop overlaps): 338118 Mohave Plant CACTUS, SILER PINCUSHION Pediocactus (= Echinocactus,= Utahia) Threatened known 3977 CLIFFROSE, ARIZONA Purshia (= cowania) subintegra Endangered known 3977 CYCLADENIA, JONES Cycladenia jonesii (= humilis) Threatened known 3977 MILK VETCH, HOLMGREN Astragalus holmgreniorum Endangered known 3977 Crop Acres ( inclusive of species crop overlaps): 15908 Pima Plant BLUE STAR, KEARNEY'S Amsonia kearneyana Endangered known 14570 CACTUS, NICHOL'S TURK'S HEAD Echinocactus horizonthalonius var. Endangered known 14570 CACTUS, PIMA PINEAPPLE Coryphantha scheeri var. robustispina Endangered possible 14570 UMBEL, HUACHUCA WATER Lilaeopsis schaffneriana var. recurva Endangered known 14570 Crop Acres ( inclusive of species crop overlaps): 58280 Pinal  139 Plant CACTUS, ARIZONA HEDGEHOG Echinocereus triglochidiatus var. Endangered known 118608 CACTUS, NICHOL'S TURK'S HEAD Echinocactus horizonthalonius var. Endangered known 118608 Crop Acres ( inclusive of species crop overlaps): 237216 California County County Status Presence Acres Monday, October 04, 2004 Page 5 of 31  140 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres California County County Status Presence Acres Colusa Plant BIRD'S BEAK, PALMATE BRACTED Cordylanthus palmatus Endangered known 7483 Crop Acres ( inclusive of species crop overlaps): 7483 Fresno Plant ADOBE SUNBURST, SAN JOAQUIN Pseudobahia peirsonii Endangered known 348003 BIRD'S BEAK, PALMATE BRACTED Cordylanthus palmatus Endangered known 348003 CHECKER MALLOW, KECK'S Sidalcea keckii Endangered known 348003 DUDLEYA, SANTA CLARA VALLEY Dudleya setchellii Endangered possible 348003 GOLDEN SUNBURST, HARTWEG'S Pseudobahia bahiifolia Endangered known 348003 GRASS, SAN JOAQUIN VALLEY Orcuttia inaequalis Threatened known 348003 ORCUTT JEWELFLOWER, CALIFORNIA Caulanthus californicus Endangered known 348003 OWL'S CLOVER, FLESHY Castilleja campestris ssp. succulenta Endangered known 348003 PUSSYPAWS, MARIPOSA Calyptridium pulchellum Threatened known 348003 WOOLLY THREADS, SAN JOAQUIN Monolopia (= Lembertia) congdonii Endangered known 348003 Crop Acres ( inclusive of species crop overlaps): 3480030 Glenn Plant  141 GRASS, HAIRY ORCUTT Orcuttia pilosa Endangered known 1046 SPURGE, HOOVER'S Chamaesyce hooveri Threatened known 1046 TUCTORIA, GREEN'S Tuctoria greenei Endangered known 1046 Crop Acres ( inclusive of species crop overlaps): 3138 Imperial Plant MILK VETCH, PIERSON'S Astragalus magdalenae var. peirsonii Threatened known 6058 Monday, October 04, 2004 Page 6 of 31  142 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres California County County Status Presence Acres Imperial Crop Acres ( inclusive of species crop overlaps): 6058 Kern Plant CACTUS, BAKERSFIELD Opuntia treleasei Endangered known 265462 JEWELFLOWER, CALIFORNIA Caulanthus californicus Endangered known 265462 MALLOW, KERN Eremalche kernensis Endangered known 265462 WOOLLY THREADS, SAN JOAQUIN Monolopia (= Lembertia) congdonii Endangered known 265462 Crop Acres ( inclusive of species crop overlaps): 1061848 Kings Plant JEWELFLOWER, CALIFORNIA Caulanthus californicus Endangered known 196108 WOOLLY THREADS, SAN JOAQUIN Monolopia (= Lembertia) congdonii Endangered known 196108 Crop Acres ( inclusive of species crop overlaps): 392216 Madera Plant ADOBE SUNBURST, SAN JOAQUIN Pseudobahia peirsonii Endangered known 36806 BIRD'S BEAK, PALMATE BRACTED Cordylanthus palmatus Endangered possible 36806 GOLDEN SUNBURST, HARTWEG'S Pseudobahia bahiifolia Endangered known 36806 GRASS, HAIRY ORCUTT Orcuttia pilosa Endangered known 36806  143 GRASS, SAN JOAQUIN VALLEY Orcuttia inaequalis Threatened known 36806 ORCUTT LUPINE, CLOVER Lupinus tidestromii Endangered known 36806 OWL'S CLOVER, FLESHY Castilleja campestris ssp. succulenta Endangered known 36806 PUSSYPAWS, MARIPOSA Calyptridium pulchellum Threatened known 36806 TUCTORIA, GREEN'S Tuctoria greenei Endangered extirpate 36806 Crop Acres ( inclusive of species crop overlaps): 331254 Monday, October 04, 2004 Page 7 of 31  144 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres California County County Status Presence Acres Merced Plant GRASS, COLUSA Neostapfia colusana Threatened known 74620 GRASS, HAIRY ORCUTT Orcuttia pilosa Endangered known 74620 GRASS, SAN JOAQUIN VALLEY Orcuttia inaequalis Threatened known 74620 ORCUTT OWL'S CLOVER, FLESHY Castilleja campestris ssp. succulenta Endangered known 74620 SPURGE, HOOVER'S Chamaesyce hooveri Threatened known 74620 TUCTORIA, GREEN'S Tuctoria greenei Endangered known 74620 Crop Acres ( inclusive of species crop overlaps): 447720 Riverside Plant AMBROSIA, SAN DIEGO Ambrosia pumila Endangered known 12571 BARBERRY, NEVIN'S Berberis nevinii Endangered known 12571 BRODIAEA, THREAD LEAVED Brodiaea filifolia Threatened known 12571 BUTTON CELERY, SAN DIEGO Eryngium aristulatum var. parishii Endangered known 12571 CEANOTHUS, VAIL LAKE Ceanothus ophiochilus Threatened known 12571 CROWNSCALE, SAN JACINTO Atriplex coronata var. notatior Endangered known 12571 VALLEY DAISY, PARISH'S Erigeron parishii Threatened known 12571 GRASS, CALIFORNIA ORCUTT Orcuttia californica Endangered known 12571  145 MILK VETCH, COACHELLA VALLEY Astragalus lentiginosus var. coachellae Endangered known 12571 MILK VETCH, TRIPLE RIBBED Astragalus tricarinatus Endangered known 12571 MINT, OTAY MESA Pogogyne nudiuscula Endangered known 12571 NAVARRETIA, SPREADING Navarretia fossalis Threatened known 12571 ONION, MUNZ'S Allium munzii Endangered known 12571 SPINEFLOWER, SLENDER HORNED Dodecahema leptoceras Endangered known 12571 WOOLLY STAR, SANTA ANA RIVER Eriastrum densifolium ssp. sanctorum Endangered known 12571 Monday, October 04, 2004 Page 8 of 31  146 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres California County County Status Presence Acres Riverside Crop Acres ( inclusive of species crop overlaps): 188565 San Bernardino Plant BLADDERPOD, SAN BERNARDINO Lesquerella kingii ssp. bernardina Endangered known 339 MOUNTAINS BLUECURLS, HIDDEN LAKE Trichostema austromontanum ssp. Threatened known 339 BLUEGRASS, SAN BERNARDINO Poa atropurpurea Endangered known 339 BRODIAEA, THREAD LEAVED Brodiaea filifolia Threatened known 339 BUCKWHEAT, CUSHENBURY Eriogonum ovalifolium var. vineum Endangered known 339 BUCKWHEAT, SOUTHERN MOUNTAIN Eriogonum kennedyi var. austromontanum Threatened known 339 WILD CHECKER MALLOW, PEDATE Sidalcea pedata Endangered known 339 DAISY, PARISH'S Erigeron parishii Threatened known 339 MILK VETCH, CUSHENBURY Astragalus albens Endangered known 339 MILK VETCH, LANE MOUNTAIN Astragalus jaegerianus Endangered known 339 MILK VETCH, TRIPLE RIBBED Astragalus tricarinatus Endangered known 339 MUSTARD, SLENDER PETALED Thelypodium stenopetalum Endangered known 339 OXYTHECA, CUSHENBURY Oxytheca parishii var. goodmaniana Endangered known 339 PAINTBRUSH, ASH GREY INDIAN Castilleja cinerea Threatened known 339 SANDWORT, BEAR VALLEY Arenaria ursina Threatened known 339  147 SPINEFLOWER, SLENDER HORNED Dodecahema leptoceras Endangered known 339 TARAXACUM, CALIFORNIA Taraxacum californicum Endangered known 339 WATERCRESS, GAMBEL'S Rorippa gambellii Endangered known 339 WOOLLY STAR, SANTA ANA RIVER Eriastrum densifolium ssp. sanctorum Endangered known 339 Crop Acres ( inclusive of species crop overlaps): 6441 Tulare Plant Monday, October 04, 2004 Page 9 of 31  148 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres California County County Status Presence Acres Tulare CHECKER MALLOW, KECK'S Sidalcea keckii Endangered known 81089 CLARKIA, SPRINGVILLE Clarkia springvillensis Threatened known 81089 GRASS, SAN JOAQUIN VALLEY Orcuttia inaequalis Threatened known 81089 ORCUTT JEWELFLOWER, CALIFORNIA Caulanthus californicus Endangered known 81089 SPURGE, HOOVER'S Chamaesyce hooveri Threatened known 81089 WOOLLY THREADS, SAN JOAQUIN Monolopia (= Lembertia) congdonii Endangered known 81089 Crop Acres ( inclusive of species crop overlaps): 486534 Yolo Plant BIRD'S BEAK, PALMATE BRACTED Cordylanthus palmatus Endangered known 4057 GRASS, COLUSA Neostapfia colusana Threatened known 4057 GRASS, SOLANO Tuctoria mucronata Endangered known 4057 Crop Acres ( inclusive of species crop overlaps): 12171 Florida County County Status Presence Acres Calhoun Plant PINKROOT, GENTIAN Spigelia gentianoides Endangered known 6600  149 Crop Acres ( inclusive of species crop overlaps): 6600 Jackson Plant PINKROOT, GENTIAN Spigelia gentianoides Endangered known 23273 TORREYA, FLORIDA Torreya taxifolia Endangered known 23273 Crop Acres ( inclusive of species crop overlaps): 46546 Monday, October 04, 2004 Page 10 of 31  150 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Florida County County Status Presence Acres Jefferson Plant GOOSEBERRY, MICCOSUKEE Ribes echinellum Threatened known 1477 ( FLORIDA)

Crop Acres ( inclusive of species crop overlaps): 1477 Okaloosa Plant CLADONIA, FLORIDA PERFORATE Cladonia perforata Endangered known 3796 Crop Acres ( inclusive of species crop overlaps): 3796 Walton Plant MEADOWRUE, COOLEY'S Thalictrum cooleyi Endangered known 3711 Crop Acres ( inclusive of species crop overlaps): 3711 Georgia County County Status Presence Acres Bartow Plant GRASS, TENNESSEE YELLOW EYED Xyris tennesseensis Endangered known 2556 POGONIA, SMALL WHORLED Isotria medeoloides Threatened possible 2556  151 Crop Acres ( inclusive of species crop overlaps): 5112 Bibb Plant CAMPION, FRINGED Silene polypetala Endangered known 305 TRILLIUM, RELICT Trillium reliquum Endangered known 305 Crop Acres ( inclusive of species crop overlaps): 610 Monday, October 04, 2004 Page 11 of 31  152 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Georgia County County Status Presence Acres Burke Plant DROPWORT, CANBY'S Oxypolis canbyi Endangered known 47843 Crop Acres ( inclusive of species crop overlaps): 47843 Clay Plant TRILLIUM, RELICT Trillium reliquum Endangered known 4753 Crop Acres ( inclusive of species crop overlaps): 4753 Crawford Plant CAMPION, FRINGED Silene polypetala Endangered known 700 Crop Acres ( inclusive of species crop overlaps): 700 Decatur Plant CAMPION, FRINGED Silene polypetala Endangered known 34720 TORREYA, FLORIDA Torreya taxifolia Endangered known 34720 Crop Acres ( inclusive of species crop overlaps): 69440 Dooly Plant  153 DROPWORT, CANBY'S Oxypolis canbyi Endangered known 69227 Crop Acres ( inclusive of species crop overlaps): 69227 Early Plant TRILLIUM, RELICT Trillium reliquum Endangered known 33630 Crop Acres ( inclusive of species crop overlaps): 33630 Monday, October 04, 2004 Page 12 of 31  154 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Georgia County County Status Presence Acres Elbert Plant SUMAC, MICHAUX'S Rhus michauxii Endangered known 2543 Crop Acres ( inclusive of species crop overlaps): 2543 Floyd Plant BARBARA'S BUTTONS, MOHR'S Marshallia mohrii Endangered known 3769 POGONIA, SMALL WHORLED Isotria medeoloides Threatened possible 3769 SKULLCAP, LARGE FLOWERED Scutellaria montana Threatened known 3769 Crop Acres ( inclusive of species crop overlaps): 11307 Henry Plant AMPHIANTHUS, LITTLE Amphianthus pusillus Threatened known 792 Crop Acres ( inclusive of species crop overlaps): 792 Jenkins Plant DROPWORT, CANBY'S Oxypolis canbyi Endangered known 12939 Crop Acres ( inclusive of species crop overlaps): 12939 Lee  155 Plant DROPWORT, CANBY'S Oxypolis canbyi Endangered known 23613 TRILLIUM, RELICT Trillium reliquum Endangered known 23613 Crop Acres ( inclusive of species crop overlaps): 47226 Macon Plant Monday, October 04, 2004 Page 13 of 31  156 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Georgia County County Status Presence Acres Macon TRILLIUM, RELICT Trillium reliquum Endangered known 16678 Crop Acres ( inclusive of species crop overlaps): 16678 Pike Plant AMPHIANTHUS, LITTLE Amphianthus pusillus Threatened known 293 QUILLWORT, BLACK SPORED Isoetes melanospora Endangered known 293 Crop Acres ( inclusive of species crop overlaps): 586 Polk Plant POGONIA, SMALL WHORLED Isotria medeoloides Threatened possible 1876 Crop Acres ( inclusive of species crop overlaps): 1876 Quitman Plant TRILLIUM, RELICT Trillium reliquum Endangered known 2795 Crop Acres ( inclusive of species crop overlaps): 2795 Screven Plant DROPWORT, CANBY'S Oxypolis canbyi Endangered known 26330  157 Crop Acres ( inclusive of species crop overlaps): 26330 Sumter Plant DROPWORT, CANBY'S Oxypolis canbyi Endangered possible 39437 Crop Acres ( inclusive of species crop overlaps): 39437 Taylor Monday, October 04, 2004 Page 14 of 31  158 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Georgia County County Status Presence Acres Taylor Plant CAMPION, FRINGED Silene polypetala Endangered known 4545 PITCHER PLANT, GREEN Sarracenia oreophila Endangered known 4545 Crop Acres ( inclusive of species crop overlaps): 9090 Wayne Plant RATTLEWEED, HAIRY Baptisia arachnifera Endangered known 11683 Crop Acres ( inclusive of species crop overlaps): 11683 Wheeler Plant PONDBERRY Lindera melissifolia Endangered known 2824 Crop Acres ( inclusive of species crop overlaps): 2824 Kansas County County Status Presence Acres Atchison Plant ORCHID, WESTERN PRAIRIE FRINGED Platanthera praeclara Threatened known 73  159 Crop Acres ( inclusive of species crop overlaps): 73 Missouri County County Status Presence Acres Butler Plant PONDBERRY Lindera melissifolia Endangered known 828 Monday, October 04, 2004 Page 15 of 31  160 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Missouri County County Status Presence Acres Butler Crop Acres ( inclusive of species crop overlaps): 828 Dade Plant BLADDERPOD, MISSOURI Lesquerella filiformis Endangered known 667 CLOVER, RUNNING BUFFALO Trifolium stoloniferum Endangered known 667 GEOCARPON MINIMUM Geocarpon minimum Endangered known 667 MILKWEED, MEAD'S Asclepias meadii Threatened known 667 Crop Acres ( inclusive of species crop overlaps): 2668 Dunklin Plant ASTER, DECURRENT FALSE Boltonia decurrens Threatened known 162794 CLOVER, RUNNING BUFFALO Trifolium stoloniferum Endangered known 162794 Crop Acres ( inclusive of species crop overlaps): 325588 Mississippi Plant ASTER, DECURRENT FALSE Boltonia decurrens Threatened known 2757 Crop Acres ( inclusive of species crop overlaps): 2757 Mississippi  161 County County Status Presence Acres Bolivar Plant PONDBERRY Lindera melissifolia Endangered known 63027 Crop Acres ( inclusive of species crop overlaps): 63027 Clay Monday, October 04, 2004 Page 16 of 31  162 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Mississippi County County Status Presence Acres Clay Plant POTATO BEAN, PRICE'S Apios priceana Threatened known 2462 Crop Acres ( inclusive of species crop overlaps): 2462 Lee Plant POTATO BEAN, PRICE'S Apios priceana Threatened known 6722 Crop Acres ( inclusive of species crop overlaps): 6722 Oktibbeha Plant POTATO BEAN, PRICE'S Apios priceana Threatened known 702 Crop Acres ( inclusive of species crop overlaps): 702 Sharkey Plant PONDBERRY Lindera melissifolia Endangered known 34603 Crop Acres ( inclusive of species crop overlaps): 34603 Sunflower Plant PONDBERRY Lindera melissifolia Endangered known 58271  163 Crop Acres ( inclusive of species crop overlaps): 58271 North Carolina County County Status Presence Acres Beaufort Plant JOINT VETCH, SENSITIVE Aeschynomene virginica Threatened known 4602 Monday, October 04, 2004 Page 17 of 31  164 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres North Carolina County County Status Presence Acres Beaufort LOOSESTRIFE, ROUGH LEAVED Lysimachia asperulaefolia Endangered possible 4602 Crop Acres ( inclusive of species crop overlaps): 9204 Bladen Plant LOOSESTRIFE, ROUGH LEAVED Lysimachia asperulaefolia Endangered known 8151 PONDBERRY Lindera melissifolia Endangered known 8151 Crop Acres ( inclusive of species crop overlaps): 16302 Cleveland Plant HEARTLEAF, DWARF FLOWERED Hexastylis naniflora Threatened known 2875 Crop Acres ( inclusive of species crop overlaps): 2875 Columbus Plant LOOSESTRIFE, ROUGH LEAVED Lysimachia asperulaefolia Endangered possible 2039 Crop Acres ( inclusive of species crop overlaps): 2039 Craven Plant JOINT VETCH, SENSITIVE Aeschynomene virginica Threatened possible 17556  165 Crop Acres ( inclusive of species crop overlaps): 17556 Cumberland Plant LOOSESTRIFE, ROUGH LEAVED Lysimachia asperulaefolia Endangered known 3925 PONDBERRY Lindera melissifolia Endangered known 3925 SUMAC, MICHAUX'S Rhus michauxii Endangered known 3925 Monday, October 04, 2004 Page 18 of 31  166 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres North Carolina County County Status Presence Acres Cumberland Crop Acres ( inclusive of species crop overlaps): 11775 Franklin Plant SUMAC, MICHAUX'S Rhus michauxii Endangered known 182 Crop Acres ( inclusive of species crop overlaps): 182 Granville Plant CONEFLOWER, SMOOTH Echinacea laevigata Endangered known 96 HARPERELLA Ptilimnium nodosum Endangered known 96 Crop Acres ( inclusive of species crop overlaps): 192 Hoke Plant CHAFFSEED, AMERICAN Schwalbea americana Endangered known 18645 LOOSESTRIFE, ROUGH LEAVED Lysimachia asperulaefolia Endangered known 18645 SUMAC, MICHAUX'S Rhus michauxii Endangered known 18645 Crop Acres ( inclusive of species crop overlaps): 55935 Hyde Plant  167 AMARANTH, SEABEACH Amaranthus pumilus Threatened known 4212 JOINT VETCH, SENSITIVE Aeschynomene virginica Threatened known 4212 Crop Acres ( inclusive of species crop overlaps): 8424 Martin Plant HEATHER, MOUNTAIN GOLDEN Hudsonia montana Threatened known 37139 Monday, October 04, 2004 Page 19 of 31  168 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres North Carolina County County Status Presence Acres Martin Crop Acres ( inclusive of species crop overlaps): 37139 Onslow Plant AMARANTH, SEABEACH Amaranthus pumilus Threatened known 3600 LOOSESTRIFE, ROUGH LEAVED Lysimachia asperulaefolia Endangered known 3600 MEADOWRUE, COOLEY'S Thalictrum cooleyi Endangered known 3600 SEDGE, GOLDEN Carex lutea Endangered known 3600 Crop Acres ( inclusive of species crop overlaps): 14400 Pamlico Plant LOOSESTRIFE, ROUGH LEAVED Lysimachia asperulaefolia Endangered known 5708 Crop Acres ( inclusive of species crop overlaps): 5708 Robeson Plant SUMAC, MICHAUX'S Rhus michauxii Endangered known 30193 Crop Acres ( inclusive of species crop overlaps): 30193 Rutherford Plant  169 HEARTLEAF, DWARF FLOWERED Hexastylis naniflora Threatened known 341 IRISETTE, WHITE Sisyrinchium dichotomum Endangered known 341 LICHEN, ROCK GNOME Gymnoderma lineare Endangered known 341 Crop Acres ( inclusive of species crop overlaps): 1023 Sampson Plant PONDBERRY Lindera melissifolia Endangered known 42202 Monday, October 04, 2004 Page 20 of 31  170 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres North Carolina County County Status Presence Acres Sampson Crop Acres ( inclusive of species crop overlaps): 42202 Scotland Plant DROPWORT, CANBY'S Oxypolis canbyi Endangered known 14033 LOOSESTRIFE, ROUGH LEAVED Lysimachia asperulaefolia Endangered known 14033 SUMAC, MICHAUX'S Rhus michauxii Endangered known 14033 Crop Acres ( inclusive of species crop overlaps): 42099 Stanly Plant SUNFLOWER, SCHWEINITZ'S Helianthus schweinitzii Endangered known 6380 Crop Acres ( inclusive of species crop overlaps): 6380 Tyrrell Plant SPIRAEA, VIRGINIA Spiraea virginiana Threatened known 1311 Crop Acres ( inclusive of species crop overlaps): 1311 Union Plant SUNFLOWER, SCHWEINITZ'S Helianthus schweinitzii Endangered known 7798  171 Crop Acres ( inclusive of species crop overlaps): 7798 New Mexico County County Status Presence Acres Chaves Plant CACTUS, KUENZLER HEDGEHOG Echinocereus fendleri var. kuenzleri Endangered known 6954 Monday, October 04, 2004 Page 21 of 31  172 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres New Mexico County County Status Presence Acres Chaves SUNFLOWER, PECOS Helianthus paradoxus Threatened known 6954 Crop Acres ( inclusive of species crop overlaps): 13908 Dona Ana Plant CACTUS, SNEED PINCUSHION Coryphantha sneedii var. sneedii Endangered known 22016 Crop Acres ( inclusive of species crop overlaps): 22016 Eddy Plant CACTUS, LEE PINCUSHION Coryphantha sneedii var. leei Threatened known 9808 WILD BUCKWHEAT, GYPSUM Eriogonum gypsophilum Threatened known 9808 Crop Acres ( inclusive of species crop overlaps): 19616 South Carolina County County Status Presence Acres Aiken Plant CONEFLOWER, SMOOTH Echinacea laevigata Endangered possible 4519 HARPERELLA Ptilimnium nodosum Endangered known 4519 TRILLIUM, RELICT Trillium reliquum Endangered known 4519  173 Crop Acres ( inclusive of species crop overlaps): 13557 Allendale Plant CONEFLOWER, SMOOTH Echinacea laevigata Endangered possible 6146 DROPWORT, CANBY'S Oxypolis canbyi Endangered known 6146 Crop Acres ( inclusive of species crop overlaps): 12292 Monday, October 04, 2004 Page 22 of 31  174 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres South Carolina County County Status Presence Acres Bamberg Plant DROPWORT, CANBY'S Oxypolis canbyi Endangered known 11699 Crop Acres ( inclusive of species crop overlaps): 11699 Barnwell Plant CONEFLOWER, SMOOTH Echinacea laevigata Endangered possible 6718 DROPWORT, CANBY'S Oxypolis canbyi Endangered known 6718 Crop Acres ( inclusive of species crop overlaps): 13436 Berkeley Plant CHAFFSEED, AMERICAN Schwalbea americana Endangered known 817 PONDBERRY Lindera melissifolia Endangered known 817 Crop Acres ( inclusive of species crop overlaps): 1634 Chesterfield Plant LOOSESTRIFE, ROUGH LEAVED Lysimachia asperulaefolia Endangered possible 752 Crop Acres ( inclusive of species crop overlaps): 752 Clarendon  175 Plant CHAFFSEED, AMERICAN Schwalbea americana Endangered known 12818 DROPWORT, CANBY'S Oxypolis canbyi Endangered known 12818 Crop Acres ( inclusive of species crop overlaps): 25636 Colleton Plant Monday, October 04, 2004 Page 23 of 31  176 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres South Carolina County County Status Presence Acres Colleton PONDBERRY Lindera melissifolia Endangered possible 1358 Crop Acres ( inclusive of species crop overlaps): 1358 Darlington Plant LOOSESTRIFE, ROUGH LEAVED Lysimachia asperulaefolia Endangered possible 23471 Crop Acres ( inclusive of species crop overlaps): 23471 Dorchester Plant DROPWORT, CANBY'S Oxypolis canbyi Endangered possible 7077 Crop Acres ( inclusive of species crop overlaps): 7077 Edgefield Plant TRILLIUM, RELICT Trillium reliquum Endangered known 3380 Crop Acres ( inclusive of species crop overlaps): 3380 Florence Plant CHAFFSEED, AMERICAN Schwalbea americana Endangered possible 8607 Crop Acres ( inclusive of species crop overlaps): 8607  177 Georgetown Plant AMARANTH, SEABEACH Amaranthus pumilus Threatened known 469 PONDBERRY Lindera melissifolia Endangered possible 469 Crop Acres ( inclusive of species crop overlaps): 938 Hampton Monday, October 04, 2004 Page 24 of 31  178 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres South Carolina County County Status Presence Acres Hampton Plant DROPWORT, CANBY'S Oxypolis canbyi Endangered known 14186 Crop Acres ( inclusive of species crop overlaps): 14186 Horry Plant AMARANTH, SEABEACH Amaranthus pumilus Threatened possible 1065 CHAFFSEED, AMERICAN Schwalbea americana Endangered known 1065 DROPWORT, CANBY'S Oxypolis canbyi Endangered possible 1065 LOOSESTRIFE, ROUGH LEAVED Lysimachia asperulaefolia Endangered possible 1065 PONDBERRY Lindera melissifolia Endangered possible 1065 SUNFLOWER, SCHWEINITZ'S Helianthus schweinitzii Endangered possible 1065 Crop Acres ( inclusive of species crop overlaps): 6390 Lee Plant CHAFFSEED, AMERICAN Schwalbea americana Endangered known 25588 Crop Acres ( inclusive of species crop overlaps): 25588 Lexington Plant CONEFLOWER, SMOOTH Echinacea laevigata Endangered possible 3635  179 SUNFLOWER, SCHWEINITZ'S Helianthus schweinitzii Endangered possible 3635 Crop Acres ( inclusive of species crop overlaps): 7270 Marlboro Plant DROPWORT, CANBY'S Oxypolis canbyi Endangered possible 32426 LOOSESTRIFE, ROUGH LEAVED Lysimachia asperulaefolia Endangered possible 32426 Monday, October 04, 2004 Page 25 of 31  180 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres South Carolina County County Status Presence Acres Marlboro Crop Acres ( inclusive of species crop overlaps): 64852 Orangeburg Plant DROPWORT, CANBY'S Oxypolis canbyi Endangered known 36355 Crop Acres ( inclusive of species crop overlaps): 36355 Saluda Plant AMPHIANTHUS, LITTLE Amphianthus pusillus Threatened known 1253 HARPERELLA Ptilimnium nodosum Endangered known 1253 Crop Acres ( inclusive of species crop overlaps): 2506 Sumter Plant CHAFFSEED, AMERICAN Schwalbea americana Endangered known 9958 Crop Acres ( inclusive of species crop overlaps): 9958 Williamsburg Plant CHAFFSEED, AMERICAN Schwalbea americana Endangered known 23625 DROPWORT, CANBY'S Oxypolis canbyi Endangered known 23625  181 Crop Acres ( inclusive of species crop overlaps): 47250 York Plant AMPHIANTHUS, LITTLE Amphianthus pusillus Threatened known 3546 SUNFLOWER, SCHWEINITZ'S Helianthus schweinitzii Endangered known 3546 Crop Acres ( inclusive of species crop overlaps): 7092 Monday, October 04, 2004 Page 26 of 31  182 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Tennessee County County Status Presence Acres Rutherford Plant CONEFLOWER, TENNESSEE PURPLE Echinacea tennesseensis Endangered known 816 GROUND PLUM, GUTHRIE'S Astragalus bibullatus Endangered known 816 PRAIRIE CLOVER, LEAFY Dalea foliosa Endangered known 816 ROCK CRESS, LARGE (= BRAUN'S) Arabis perstellata Endangered known 816 Crop Acres ( inclusive of species crop overlaps): 3264 Texas County County Status Presence Acres Brazos Plant LADIES' TRESSES, NAVASOTA Spiranthes parksii Endangered known 5939 Crop Acres ( inclusive of species crop overlaps): 5939 Burleson Plant LADIES' TRESSES, NAVASOTA Spiranthes parksii Endangered known 15376 Crop Acres ( inclusive of species crop overlaps): 15376 Coke  183 Plant POPPY MALLOW, TEXAS Callirhoe scabriuscula Endangered known 838 Crop Acres ( inclusive of species crop overlaps): 838 Culberson Plant CACTUS, SNEED PINCUSHION Coryphantha sneedii var. sneedii Endangered known 869 Monday, October 04, 2004 Page 27 of 31  184 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Texas County County Status Presence Acres Culberson Crop Acres ( inclusive of species crop overlaps): 869 El Paso Plant CACTUS, SNEED PINCUSHION Coryphantha sneedii var. sneedii Endangered known 27103 Crop Acres ( inclusive of species crop overlaps): 27103 Fort Bend Plant DAWN FLOWER, TEXAS PRAIRIE Hymenoxys texana Endangered known 46380 (= TEXAS BITTERWEED Crop Acres ( inclusive of species crop overlaps): 46380 Hays Plant WILD RICE, TEXAS Zizania texana Endangered known 210 Crop Acres ( inclusive of species crop overlaps): 210 Hidalgo Plant AMBROSIA, SOUTH TEXAS Ambrosia cheiranthifolia Endangered known 61890 AYENIA, TEXAS Ayenia limitaris Endangered possible 61890 MANIOC, WALKER'S Manihot walkerae Endangered known 61890  185 Crop Acres ( inclusive of species crop overlaps): 185670 Hudspeth Plant CACTUS, SNEED PINCUSHION Coryphantha sneedii var. sneedii Endangered known 9430 Crop Acres ( inclusive of species crop overlaps): 9430 Jim Wells Monday, October 04, 2004 Page 28 of 31  186 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Texas County County Status Presence Acres Jim Wells Plant CACTUS, BLACK LACE Echinocereus reichenbachii var. albertii Endangered known 7075 Crop Acres ( inclusive of species crop overlaps): 7075 Kleberg Plant AMBROSIA, SOUTH TEXAS Ambrosia cheiranthifolia Endangered known 38070 AYENIA, TEXAS Ayenia limitaris Endangered possible 38070 CACTUS, BLACK LACE Echinocereus reichenbachii var. albertii Endangered known 38070 RUSH PEA, SLENDER Hoffmannseggia tenella Endangered known 38070 Crop Acres ( inclusive of species crop overlaps): 152280 Mitchell Plant POPPY MALLOW, TEXAS Callirhoe scabriuscula Endangered known 60127 Crop Acres ( inclusive of species crop overlaps): 60127 Nueces Plant AMBROSIA, SOUTH TEXAS Ambrosia cheiranthifolia Endangered known 100597 AYENIA, TEXAS Ayenia limitaris Endangered possible 100597 RUSH PEA, SLENDER Hoffmannseggia tenella Endangered known 100597  187 Crop Acres ( inclusive of species crop overlaps): 301791 Pecos Plant SUNFLOWER, PECOS Helianthus paradoxus Threatened known 8767 Crop Acres ( inclusive of species crop overlaps): 8767 Reeves Monday, October 04, 2004 Page 29 of 31  188 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Texas County County Status Presence Acres Reeves Plant SUNFLOWER, PECOS Helianthus paradoxus Threatened known 6620 Crop Acres ( inclusive of species crop overlaps): 6620 Refugio Plant CACTUS, BLACK LACE Echinocereus reichenbachii var. albertii Endangered known 21044 Crop Acres ( inclusive of species crop overlaps): 21044 Robertson Plant LADIES' TRESSES, NAVASOTA Spiranthes parksii Endangered known 12082 SAND VERBENA, LARGE FRUITED Abronia macrocarpa Endangered known 12082 Crop Acres ( inclusive of species crop overlaps): 24164 Runnels Plant POPPY MALLOW, TEXAS Callirhoe scabriuscula Endangered known 46188 Crop Acres ( inclusive of species crop overlaps): 46188 Starr Plant  189 BLADDERPOD, ZAPATA Lesquerella thamnophila Endangered known 4591 CACTUS, STAR Astrophytum asterias Endangered known 4591 DOGWEED, ASHY Thymophylla tephroleuca Endangered known 4591 MANIOC, WALKER'S Manihot walkerae Endangered possible 4591 Crop Acres ( inclusive of species crop overlaps): 18364 Uvalde Monday, October 04, 2004 Page 30 of 31  190 Species Listing by State for Crop Cotton ( 43)

Minimum of 1 Acres Texas County County Status Presence Acres Uvalde Plant CACTUS, BLACK LACE Echinocereus reichenbachii var. albertii Endangered known 5990 CACTUS, TOBUSCH FISHHOOK Ancistrocactus tobuschii Endangered known 5990 SNOWBELLS, TEXAS Styrax texanus Endangered known 5990 Crop Acres ( inclusive of species crop overlaps): 17970 Virginia County County Status Presence Acres Charles City Plant JOINT VETCH, SENSITIVE Aeschynomene virginica Threatened known 1915 Crop Acres ( inclusive of species crop overlaps): 1915 Dinwiddie Plant SUMAC, MICHAUX'S Rhus michauxii Endangered known 3446 Crop Acres ( inclusive of species crop overlaps): 3446 King and Queen Plant JOINT VETCH, SENSITIVE Aeschynomene virginica Threatened known 1019  191 Crop Acres ( inclusive of species crop overlaps): 1019 Surry Plant JOINT VETCH, SENSITIVE Aeschynomene virginica Threatened known 2926 Crop Acres ( inclusive of species crop overlaps): 2926 Monday, October 04, 2004 Page 31 of 31  192 Appendix H. Environmental Fate Bibliography Dzialo, D. G. 1992. [ 14C] Harvade Aerobic Soil Metabolism on Sandy Loam Soil. Unpublished study performed and submitted by Uniroyal Chemical Company, Inc. Middlebury, CT. Laboratory Project ID: 9054. MRID 42429606.

Dzialo, D. G. 1993. [ 14C] Harvade Anaerobic Aquatic Metabolism. Unpublished study performed, sponsored and submitted by Uniroyal Chemical Company, Inc., Middlebury, CT. Uniroyal Project No. 9018. MRID 42673501.

Dzialo, D. G. 1994. Field Dissipation of Dimethipin ( Harvade 5F) on Cotton in Georgia. Unpublished study performed by Landis International, Inc., Valdosta, GA, Research Options, Inc., Montezuma, GA, NET, Inc.   Cambridge Division, Bedford, MA, and Biodevelopment Laboratories, Inc., Cambridge, MA, and submitted by Uniroyal Chemical Company, Inc., Middlebury, CT. Laboratory Project ID: 9113/ 9175. MRID 43216001.

Dzialo, D. G. 1994. Field Dissipation of Dimethipin ( Harvade 5F) on Cotton in Mississippi. Unpublished study performed by Landis International, Inc., Valdosta, GA, Stoneville Associates, Inc., Greenville, MS, NET Atlantic, Inc.   Cambridge Division, Bedford, MA, and Biodevelopment Laboratories, Inc., Cambridge, MA, and submitted by Uniroyal Chemical Company, Inc., Middlebury, CT. Laboratory Project ID: 9114/ 9176. MRID 43216002.

Fackler, P. H. 1991. Harvade  Determination of Aqueous Photolysis Rate Constant and Half life. Unpublished study performed by Springborn Laboratories, Inc., Wareham, MA, and sponsored and submitted by Uniroyal Chemical Company, Middlebury, CT. SLI Report No.: 91 7 3830. Study No.: 41.0391.6121.720. MRID 41967101.

Fackler, P. H. 1992. Harvade  Determination of Soil Photolysis Rate Constant and Half life. Unpublished study performed by Springborn Laboratories, Inc., Wareham, MA; sponsored by Uniroyal Chemical Company, Inc., Middlebury, CT; and submitted by Uniroyal Chemical Company, Inc., Bethany, CT. Uniroyal Project No. 9117. SLI Report No. 91 9 3910. SLI Study No. 41.0391.6120.721. MRID 42237601.

Fitzpatrick, K. C. 1981. Hydrolysis of Harvade  Part " A", Half life Determinations. Unpublished study performed by Raven Research Inc. ( no address provided), and submitted by Uniroyal Chemical, Crop Protection Research, Analytical Research. Project No.: 7603 A. MRID 80106.  193 Spare, W. C. 1990. Adsorption/ Desorption of 14C Harvade. Unpublished study performed by Agrisearch Incorporated, Frederick, MD; sponsored and submitted by Uniroyal Chemical Company, Incorporated, Middlebury, CT. Agrisearch Project No.: 1917. Uniroyal Project No.: 9063. MRID 41660901.  194 Appendix I. Ecotoxicity Bibliography Ames, R. B. 1977. Harvade Sensitive Crop Study, Uniroyal Chem ( pp. 90   95)/ Harvade Sensitivity Study Final Report ( pp. 96   98). Unpublished study performed by   not reported in DER. Laboratory Report Identification   not reported in DER. Study submitted by Uniroyal Chemical Company, Inc., Bethany, CT. MRID 097014.

Cole, J. H. 1985. The Acute Oral and Contact Toxicity to Honey Bees of Harvade 25F. Unpublished study performed by Huntingdon Research Centre, Ltd., Huntingdon, England. Laboratory Report Identification   not reported in DER. Study submitted by Uniroyal Chemical Company, Inc., Bethany, CT. MRID 41264606.

Czech, P. 2003. Acute Toxicity of Harvade 25F to Daphnia magna in a 48 hour Immobilization Test. Unpublished study performed by RCC Ltd., Itingen, Switzerland. Laboratory Report Identification 842758. Study submitted by Crompton Europe Limited, Berkshire, U. K. MRID 46063401.

Forbis, A. D., L. Franklin and K. Lawman. 1982. Chronic Toxicity of Harvade to Daphnia magna Under Flow through Conditions. Unpublished study performed by Analytical BioChemistry Laboratories Inc., Columbia, MO. Laboratory Report Identification   not reported in DER. Study submitted by Uniroyal Chemical Company, Bethany, CT. MRID not provided in DER ( Bar Code = 2006914).

Forbis, A. D. Franklin, L., Lawman, K. 1982. Chronic Toxicity of Harvade to Daphnia magna Under Flow Through Conditions. Prepared by Analytical Bio Chemistry Laboratories Inc., Colombia, MO, for Uniroyal Chemical, Bethany, Conn. MRID 00128803.

HED Chapter Reference ( no date): Dimethipin: HED Chapter of the Reregistration Eligibility Decision Document ( RED). PC Code: 118901, Case #: 3063, DP Barcode: D299124. Includes MRIDs 42429601 and MRID 43897601.

LeBlanc, G. A. 1977. Acute Toxicity of N252 ( Harvade Technical) to the Water Flea ( Daphnia magna). Unpublished study performed by EG & G, Bionomics, Aquatic Toxicology Laboratory, Wareham, ME. Laboratory Report Identification   not reported in DER ( Test ID No. 097014). Study submitted by Uniroyal Chemical Company, Bethany, CT. MRID not provided in DER ( Bar Code = 2006909).

McElwee, C. 1993. Harvade ® Technical: Acute Effect on New Shell Growth of the Eastern Oyster, Crassostrea virginica, Under Flow through Conditions. Unpublished study performed by Toxikon Environmental Sciences, Jupiter, FL. Laboratory Report Identification J9210002. Study submitted by  195 Uniroyal Chemical Company, Bethany, CT. MRID 42643101.

McElwee, C. 1993. Harvade Technical: Acute Toxicity to the Freshwater Blue green Algae, Anabaena flos aquae, Under Static test Conditions. Unpublished study performed by Toxikon Environmental Sciences, Jupiter, FL. Laboratory Report Identification J9209004d. Study submitted by Uniroyal Chemical Company, Bethany, CT. MRID 42627101.

McElwee, C. 1993. Harvade Technical: Acute Toxicity to the Freshwater Diatom, Nitzschia palea, Under Static test Conditions. Unpublished study performed by Toxikon Environmental Sciences, Jupiter, FL. Laboratory Report Identification J9209004e. Study submitted by Uniroyal Chemical Company, Bethany, CT. MRID 42627102.

McElwee, C. 1993. Harvade Technical: Acute Toxicity to the Saltwater Diatom, Skeletonema costatum, Under Static Test Conditions. Unpublished study performed by Toxikon Environmental Sciences, Jupiter, FL. Laboratory Report Identification J9209004f. Study submitted by Uniroyal Chemical Company, Bethany, CT. MRID 42627103.

McElwee, C. 1993. Harvade Technical: Acute Toxicity to Duckweed, Lemna gibba G3, Under Static Test Conditions. Unpublished study performed by Toxikon Environmental Sciences, Jupiter, FL. Laboratory Report Identification J9209004g. Study submitted by Uniroyal Chemical Company, Bethany, CT. MRID 42627104.

Pederson, C. A. 1991. Harvade Technical: 8 day Acute Dietary LC50 Study in Bobwhite Quail. Unpublished study performed by Bio Life Associates, Ltd., Neillsville, WI. Laboratory Report Identification   not reported in DER. Study submitted by Uniroyal Chemical Company, Bethany, CT. MRID 41945901.

Pederson, C. A. 1991. Harvade Technical: 8 day Acute Dietary LC50 Study in Mallard Ducklings. Unpublished study performed by Bio Life Associates, Ltd., Neillsville, WI. Laboratory Report Identification   not reported in DER. Study submitted by Uniroyal Chemical Company, Bethany, CT. MRID 41955902.

Pederson, C. A. 1991. Harvade Technical: Acute Oral LD50 Study in Mallard Ducks. Unpublished study performed by Bio Life Associates, Ltd., Neillsville, WI. Laboratory Report Identification   not reported in DER. Study submitted by Uniroyal Chemical Company, Bethany, CT. MRID 41955901.

Richards, C. and F. E. Kaiser. 1982. Acute Toxicity of Technical Grade Harvade to Selenastrum  196 capricornutum Printz. Unpublished study performed by Analytical BioChemistry Laboratories Inc., Columbia, MO. Laboratory Report Identification 29462. Study submitted by Uniroyal Chemical Company, Bethany, CT. MRID 41264605.

Suprenant, D. C. and Altshul. 1982. The Toxicity of Harvade to Water Flea ( Daphnia magna). Unpublished study performed by EG & G, Bionomics, Aquatic Toxicology Laboratory, Wareham, ME. Laboratory Report Identification BW 82 4 1159. Study submitted by Uniroyal Chemical Company, Bethany, CT. MRID 00126070.

Ward, G. S. 1990. Harvade ® Technical: Acute Effect on New Shell Growth of the Eastern Oyster ( Crassostrea virginica) Under Flow through Conditions. Unpublished study performed by Toxikon Environmental, Jupiter, FL. Laboratory Report Identification   not reported in DER. Study submitted by Uniroyal Chemical Company, Bethany, CT. MRID 41666501.

Ward, G. S. 1990. Acute Toxicity to Mysid, Mysidopsis bahia, Under Flow through Test Conditions. Unpublished study performed by Toxikon Environmental. Laboratory Report Identification   not reported in DER. Study submitted by Uniroyal Chemical Company, Bethany, CT. MRID 41663902.

Ward, G. S. 1990. Harvade Technical: Acute Toxicity to Rainbow Trout ( Oncorynchus mykiss) Under Flow through Test Conditions. Unpublished study performed by Toxikon Environmental, Jupiter, FL. Laboratory Report Identification   not reported in DER. Study submitted by Uniroyal Chemical Company, Bethany, CT. MRID 41945903.

Ward, G. S. 1991. Harvade Technical: Acute Toxicity to Sheepshead Minnow ( Cyprinodon variegatus) Under Flow through Test Conditions. Unpublished study performed by Toxikon Environmental, Jupiter, FL. Laboratory Report Identification   not reported in DER. Study submitted by Uniroyal Chemical Company, Bethany, CT. MRID 41663901.

Ward, G. S. 1991. Harvade Technical: Acute Toxicity to Bluegill ( Lepomis macrochirus) Under Flowthrough Test Conditions. Unpublished study performed by Toxikon Environmental, Jupiter, FL. Laboratory Report Identification   not reported in DER. Study submitted by Uniroyal Chemical Company, Bethany, CT. MRID 41945902.

Ward, G. S. 1991. Harvade Technical: Acute Toxicity to the Water Flea ( Dapnia magna) Under Flowthrough Test Conditions. Unpublished study performed by Toxikon Environmental, Jupiter, FL. Laboratory Report Identification   not reported in DER. Study submitted by Uniroyal Chemical Company, Bethany, CT. MRID 41945904.  197 Invalid Studies Blem, A. R. 1981. Response of Greenhouse grown Plants to Harvade ® Applied as an Irrigation Water Contaminant and as a Spray Drift to Freshly Sown Soil or to Plant Foliage. Unpublished study performed by Uniroyal Chemical. Laboratory Report Identification   not reported. Study submitted by Uniroyal Chemical. MRID 00085676; Acc. No. 070239.

Suprenant, D. C. and Altshul. 1982. The Toxicity of Harvade to water Flea ( Daphnia magna). Unpublished study performed by EG & G, Bionomics, Aquatic Toxicology Laboratory, Wareham, ME. Laboratory Report Identification BW 82 4 1159. Study submitted by Uniroyal Chemical Company, Bethany, CT. MRID 00126070.

Wilson, B. F. and Altshul. 1982. The Toxicity of Harvade to Fathead Minnow ( Pimephales promelas) Embryos and Larvae. Unpublished study performed by EG & G, Bionomics, Aquatic Toxicology Laboratory, Wareham, ME. Laboratory Report Identification BW 82 4 1147. Study submitted by Uniroyal Chemical Company, Bethany, CT. MRID 00126069.