1126-95.
29. Dioxathion.....................  GC....................  614.1, 1657...........  .....................  .....................  See footnote,\4\ page
                                                                                27; See footnote,\6\
                                                                                p. S73.
30. Disulfoton.....................  GC....................  507, 614, 622, 1657...  .....................  .....................  See footnote,\3\ p.
                                                                                25; See footnote,\6\
                                                                                p. S51.
                                     GC/MS.................  525.2, 625.1..........  .....................  .....................  See footnote,\11\ O-
                                                                                1126-95.
31. Diuron.........................  TLC...................  ......................  .....................  .....................  See footnote,\3\ p.
                                                                                104; See
                                                                                footnote,\6\ p. S64.

                                     HPLC..................  632...................
                                     HPLC/MS...............  553...................  .....................  .....................  See footnote,\12\ O-
                                                                                2060-01.
32. Endosulfan I...................  GC....................  617, 608.3............  6630 B-2007 & C-2007.  D3086-90, D5812-       See footnote,\3\ p.
                                                                                96(02).                7; See footnote,\4\
                                                                                O-3104-83; See
                                                                                footnote,\8\ 3M0222.
                                     GC/MS.................  625.1 \5\.............  6410 B-2000..........  .....................  See footnote,\13\ O-
                                                                                2002-01.
33. Endosulfan II..................  GC....................  617, 608.3............  6630 B-2007 & C-2007.  D3086-90, D5812-       See footnote,\3\ p.
                                                                                96(02).                7; See footnote,\8\
                                                                                3M0222.
                                     GC/MS.................  625.1 \5\.............  6410 B-2000..........  .....................  See footnote,\13\ O-
                                                                                2002-01.
34. Endosulfan Sulfate.............  GC....................  617, 608.3............  6630 C-2007..........  .....................  See footnote,\8\
                                                                                3M0222.
                                     GC/MS.................  625.1.................  6410 B-2000..........
35. Endrin.........................  GC....................  505, 508, 617, 1656,    6630 B-2007 & C-2007.  D3086-90, D5812-       See footnote,\3\ p.
                                                              608.3.                                         96(02).                7; See footnote,\4\
                                                                                O-3104-83; See
                                                                                footnote,\8\ 3M0222.
                                     GC/MS.................  525.1, 525.2, 625.1     6410 B-2000..........
                                                              \5\.
36. Endrin aldehyde................  GC....................  617, 608.3............  6630 C-2007..........  .....................  See footnote,\8\
                                                                                3M0222.
                                     GC/MS.................  625.1.................
37. Ethion.........................  GC....................  614, 614.1,1657.......  .....................  .....................  See footnote,\4\ page
                                                                                27; See footnote,\6\
                                                                                p. S73.
                                     GC/MS.................  625.1.................  .....................  .....................  See footnote,\13\ O-
                                                                                2002-01.
38. Fenuron........................  TLC...................  ......................  .....................  .....................  See footnote,\3\ p.
                                                                                104; See
                                                                                footnote,\6\ p. S64.
                                     HPLC..................  632...................
                                     HPLC/MS...............  ......................  .....................  .....................  See footnote,\12\ O-
                                                                                2060-01.
39. Fenuron-TCA....................  TLC...................  ......................  .....................  .....................  See footnote,\3\ p.
                                                                                104; See
                                                                                footnote,\6\ p. S64.
                                     HPLC..................  632...................
40. Heptachlor.....................  GC....................  505, 508, 617, 1656,    6630 B-2007 & C-2007.  D3086-90, D5812-       See footnote,\3\ p.
                                                              608.3.                                         96(02).                7; See footnote,\4\
                                                                                O-3104-83; See
                                                                                footnote,\8\ 3M0222.
                                     GC/MS.................  525.1, 525.2, 625.1...  6410 B-2000..........
41. Heptachlor epoxide.............  GC....................  617, 608.3............  6630 B-2007 & C-2007.  D3086-90, D5812-       See footnote,\3\ p.
                                                                                96(02).                7; See footnote,\4\
                                                                                O-3104-83; See
                                                                                footnote,\6\ p. S73;
                                                                                See footnote,\8\
                                                                                3M0222.
                                     GC/MS.................  625.1.................  6410 B-2000..........
42. Isodrin........................  GC....................  617, 608.3............  6630 B-2007 & C-2007.  .....................  See footnote,\4\ O-
                                                                                3104-83; See
                                                                                footnote,\6\ p. S73.
                                     GC/MS.................  625.1.................
43. Linuron........................  GC....................  ......................  .....................  .....................  See footnote,\3\ p.
                                                                                104; See
                                                                                footnote,\6\ p. S64.
                                     HPLC..................  632...................
                                     HPLC/MS...............  553...................  .....................  .....................  See footnote,\12\ O-
                                                                                2060-01.
                                     GC/MS.................  ......................  .....................  .....................  Seeootnote,\11\ O-
                                                                                1126-95.
44. Malathion......................  GC....................  614, 1657.............  6630 B-2007..........  .....................  See footnote,\3\ p.
                                                                                25; See footnote,\6\
                                                                                p. S51.
                                     GC/MS.................  625.1.................  .....................  .....................  See footnote,\11\ O-
                                                                                1126-95.
45. Methiocarb.....................  TLC...................  ......................  .....................  .....................  See footnote,\3\ p.
                                                                                94; See footnote,\6\
                                                                                p. S60.
                                     HPLC..................  632...................
                                     HPLC/MS...............  ......................  .....................  .....................  See footnote,\12\ O-
                                                                                2060-01.
46. Methoxychlor...................  GC....................  505, 508, 608.2, 617,   6630 B-2007 & C-2007.  D3086-90, D5812-       See footnote,\3\ p.
                                                              1656, 608.3.                                   96(02).                7; See footnote,\4\
                                                                                O-3104-83; See
                                                                                footnote,\8\ 3M0222.

                                     GC/MS.................  525.1, 525.2, 625.1...  .....................  .....................  See footnote,\11\ O-
                                                                                1126-95.
47. Mexacarbate....................  TLC...................  ......................  .....................  .....................  See footnote,\3\ p.
                                                                                94; See footnote,\6\
                                                                                p. S60.
                                     HPLC..................  632...................
                                     GC/MS.................  625.1.................
48. Mirex..........................  GC....................  617, 608.3............  6630 B-2007 & C-2007.  D3086-90, D5812-       See footnote,\3\ p.
                                                                                96(02).                7; See footnote,\4\
                                                                                O-3104-83.
                                     GC/MS.................  625.1.................
49. Monuron........................  TLC...................  ......................  .....................  .....................  See footnote,\3\ p.
                                                                                104; See
                                                                                footnote,\6\ p. S64.
                                     HPLC..................  632...................
50. Monuron-TCA....................  TLC...................  ......................  .....................  .....................  See footnote,\3\ p.
                                                                                104; See
                                                                                footnote,\6\ p. S64.
                                     HPLC..................  632...................
51. Neburon........................  TLC...................  ......................  .....................  .....................  See footnote,\3\ p.
                                                                                104; See
                                                                                footnote,\6\ p. S64.
                                     HPLC..................  632...................
                                     HPLC/MS...............  ......................  .....................  .....................  See footnote,\12\ O-
                                                                                2060-01.
52. Parathion methyl...............  GC....................  614, 622, 1657........  6630 B-2007..........  .....................  See footnote,\4\ page
                                                                                27; See footnote,\3\
                                                                                p. 25.
                                     GC/MS.................  625.1.................  .....................  .....................  See footnote,\11\ O-
                                                                                1126-95.
53. Parathion ethyl................  GC....................  614...................  6630 B-2007..........  .....................  See footnote,\4\ page
                                                                                27; See footnote,\3\
                                                                                p. 25.
                                     GC/MS.................  ......................  .....................  .....................  See footnote,\11\ O-
                                                                                1126-95.
54. PCNB...........................  GC....................  608.1, 617, 608.3.....  6630 B-2007 & C-2007.  D3086-90, D5812-       See footnote,\3\ p.
                                                                                96(02).                7.
55. Perthane.......................  GC....................  617, 608.3............  .....................  D3086-90, D5812-       See footnote,\4\ O-
                                                                                96(02).                3104-83.
56. Prometon.......................  GC....................  507, 619..............  .....................  .....................  See footnote,\3\ p.
                                                                                83; See footnote,\6\
                                                                                p. S68; See
                                                                                footnote,\9\ O-3106-
                                                                                93.
                                     GC/MS.................  525.2, 625.1..........  .....................  .....................  See footnote,\11\ O-
                                                                                1126-95.
57. Prometryn......................  GC....................  507, 619..............  .....................  .....................  See footnote,\3\ p.
                                                                                83; See footnote,\6\
                                                                                p. S68; See
                                                                                footnote,\9\ O-3106-
                                                                                93.
                                     GC/MS.................  525.1, 525.2, 625.1...  .....................  .....................  See footnote,\13\ O-
                                                                                2002-01.
58. Propazine......................  GC....................  507, 619, 1656, 608.3.  .....................  .....................  See footnote,\3\ p.
                                                                                83; See footnote,\6\
                                                                                p. S68; See
                                                                                footnote,\9\ O-3106-
                                                                                93.
                                     GC/MS.................  525.1, 525.2, 625.1...
59. Propham........................  TLC...................  ......................  .....................  .....................  See footnote,\3\ p.
                                                                                104; See
                                                                                footnote,\6\ p. S64.
                                     HPLC..................  632...................
                                     HPLC/MS...............  ......................  .....................  .....................  See footnote,\12\ O-
                                                                                2060-01.
60. Propoxur.......................  TLC...................  ......................  .....................  .....................  See footnote,\3\ p.
                                                                                94; See footnote,\6\
                                                                                p. S60.
                                     HPLC..................  632...................
61. Secbumeton.....................  TLC...................  ......................  .....................  .....................  See footnote,\3\ p.
                                                                                83; See footnote,\6\
                                                                                p. S68.
                                     GC....................  619...................
62. Siduron........................  TLC...................  ......................  .....................  .....................  See footnote,\3\ p.
                                                                                104; See
                                                                                footnote,\6\ p. S64.
                                     HPLC..................  632...................
                                     HPLC/MS...............  ......................  .....................  .....................  See footnote,\12\ O-
                                                                                2060-01.
63. Simazine.......................  GC....................  505, 507, 619, 1656,    .....................  .....................  See footnote,\3\ p.
                                                              608.3.                                                                83; See footnote,\6\
                                                                                p. S68; See
                                                                                footnote,\9\ O-3106-
                                                                                93.
                                     GC/MS.................  525.1, 525.2, 625.1...  .....................  .....................  See footnote,\11\ O-
                                                                                1126-95.
64. Strobane.......................  GC....................  617, 608.3............  6630 B-2007 & C-2007.  .....................  See footnote,\3\ p.
                                                                                7.
65. Swep...........................  TLC...................  ......................  .....................  .....................  See footnote,\3\ p.
                                                                                104; See
                                                                                footnote,\6\ p. S64.

                                     HPLC..................  632...................
66. 2,4,5-T........................  GC....................  615...................  6640 B-2006..........  .....................  See footnote,\3\ p.
                                                                                115; See
                                                                                footnote,\4\ O-3105-
                                                                                83.
67. 2,4,5-TP (Silvex)..............  GC....................  615...................  6640 B-2006..........  .....................  See footnote,\3\ p.
                                                                                115; See
                                                                                footnote,\4\ O-3105-
                                                                                83.
68. Terbuthylazine.................  GC....................  619, 1656, 608.3......  .....................  .....................  See footnote,\3\ p.
                                                                                83; See footnote,\6\
                                                                                p. S68.
                                     GC/MS.................  ......................  .....................  .....................  See footnote,\13\ O-
                                                                                2002-01.
69. Toxaphene......................  GC....................  505, 508, 617, 1656,    6630 B-2007 & C-2007.  D3086-90, D5812-       See footnote,\3\ p.
                                                              608.3.                                         96(02).                7; See footnote,\8\;
                                                                                See footnote,\4\ O-
                                                                                3105-83.
                                     GC/MS.................  525.1, 525.2, 625.1...  6410 B-2000..........
70. Trifluralin....................  GC....................  508, 617, 627, 1656,    6630 B-2007..........  .....................  See footnote,\3\ p.
                                                              608.3.                                                                7; See footnote,\9\
                                                                                O-3106-93.
                                     GC/MS.................  525.2, 625.1..........  .....................  .....................  See footnote,\11\ O-
                                                                                1126-95.
--------------------------------------------------------------------------------------------------------------------------------------------------------
Table ID notes:
\1\ Pesticides are listed in this table by common name for the convenience of the reader. Additional pesticides may be found under Table IC, where
  entries are listed by chemical name.
\2\ The standardized test procedure to be used to determine the method detection limit (MDL) for these test procedures is given at Appendix B,
  Definition and Procedure for the Determination of the Method Detection Limit, of this Part 136.
\3\ Methods for Benzidine, Chlorinated Organic Compounds, Pentachlorophenol and Pesticides in Water and Wastewater. September 1978. U.S. EPA. This EPA
  publication includes thin-layer chromatography (TLC) methods.
\4\ Methods for the Determination of Organic Substances in Water and Fluvial Sediments, Techniques of Water-Resources Investigations of the U.S.
  Geological Survey, Book 5, Chapter A3. 1987. USGS.
\5\ The method may be extended to include [alpha]-BHC, [gamma]-BHC, endosulfan I, endosulfan II, and endrin. However, when they are known to exist,
  Method 608.3 is the preferred method.
\6\ Selected Analytical Methods Approved and Cited by the United States Environmental Protection Agency, Supplement to the 15th Edition of Standard
  Methods for the Examination of Water and Wastewater. 1981. American Public Health Association (APHA).
\7\ Each analyst must make an initial, one-time, demonstration of their ability to generate acceptable precision and accuracy with Methods 608.3 and
  625.1 in accordance with procedures given in Section 8.2 of each of these methods. Additionally, each laboratory, on an on-going basis, must spike and
  analyze 5% of all samples analyzed with Method 608.3 or 5% of all samples analyzed with Method 625.1 to monitor and evaluate laboratory data quality
  in accordance with Sections 8.3 and 8.4 of these methods. When the recovery of any parameter falls outside the warning limits, the analytical results
  for that parameter in the unspiked sample are suspect. The results should be reported, but cannot be used to demonstrate regulatory compliance. These
  quality control requirements also apply to the Standard Methods, ASTM Methods, and other methods cited.
\8\ Organochlorine Pesticides and PCBs in Wastewater Using Empore\TM\ Disk. Revised October 28, 1994. 3M Corporation.
\9\ Method O-3106-93 is in Open File Report 94-37, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory--Determination of
  Triazine and Other Nitrogen-Containing Compounds by Gas Chromatography With Nitrogen Phosphorus Detectors. 1994. USGS.
\10\ EPA Methods 608.1, 608.2, 614, 614.1, 615, 617, 619, 622, 622.1, 627, and 632 are found in Methods for the Determination of Nonconventional
  Pesticides in Municipal and Industrial Wastewater, EPA 821-R-92-002, April 1992, U.S. EPA. EPA Methods 505, 507, 508, 525.1, 531.1 and 553 are in
  Methods for the Determination of Nonconventional Pesticides in Municipal and Industrial Wastewater, Volume II, EPA 821-R-93-010B, 1993, U.S. EPA. EPA
  Method 525.2 is in Determination of Organic Compounds in Drinking Water by Liquid-Solid Extraction and Capillary Column Gas Chromatography/Mass
  Spectrometry, Revision 2.0, 1995, U.S. EPA. EPA methods 1656 and 1657 are in Methods for the Determination of Nonconventional Pesticides in Municipal
  and Industrial Wastewater, Volume I, EPA 821-R-93-010A, 1993, U.S. EPA. Methods 608.3 and 625.1 are available at: http://water.epa.gov/scitech/methods/cwa/methods_index.cfm (this is a placeholder for now).
\11\ Method O-1126-95 is in Open-File Report 95-181, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory--Determination
  of pesticides in water by C-18 solid-phase extraction and capillary-column gas chromatography/mass spectrometry with selected-ion monitoring. 1995.
  USGS.
\12\ Method O-2060-01 is in Water-Resources Investigations Report 01-4134, Methods of Analysis by the U.S. Geological Survey National Water Quality
  Laboratory--Determination of Pesticides in Water by Graphitized Carbon-Based Solid-Phase Extraction and High-Performance Liquid Chromatography/Mass
  Spectrometry. 2001. USGS.
\13\ Method O-2002-01 is in Water-Resources Investigations Report 01-4098, Methods of Analysis by the U.S. Geological Survey National Water Quality
  Laboratory--Determination of moderate-use pesticides in water by C-18 solid-phase extraction and capillary-column gas chromatography/mass
  spectrometry. 2001. USGS.
\14\ Method O-1121-91 is in Open-File Report 91-519, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory--Determination
  of organonitrogen herbicides in water by solid-phase extraction and capillary-column gas chromatography/mass spectrometry with selected-ion
  monitoring. 1992. USGS.

* * * * *

    Table 1F--List of Approved Methods for Pharmaceutical Pollutants
------------------------------------------------------------------------
                                                           Analytical
  Pharmaceuticals pollutants       CAS Registry No.       method number
------------------------------------------------------------------------
Acetonitrile.................                  75-05-8  1666/1671/D3371/
                                                         D3695/624.1.
n-Amyl acetate...............                 628-63-7  1666/D3695.
n-Amyl alcohol...............                  71-41-0  1666/D3695.
Benzene......................                  71-43-2  D4763/D3695/
                                                         502.2/524.2/
                                                         624.1.

n-Butyl-acetate..............                 123-86-4  1666/D3695.
tert-Butyl alcohol...........                  75-65-0  1666/624.1.
Chlorobenzene................                 108-90-7  502.2/524.2/
                                                         624.1.
Chloroform...................                  67-66-3  502.2/524.2/551/
                                                         624.1.
o-Dichlorobenzene............                  95-50-1  1625C/502.2/
                                                         524.2/624.1.
1,2-Dichloroethane...........                 107-06-2  D3695/502.2/
                                                         524.2/624.1.
Diethylamine.................                 109-89-7  1666/1671.
Dimethyl sulfoxide...........                  67-68-5  1666/1671.
Ethanol......................                  64-17-5  1666/1671/D3695/
                                                         624.1.
Ethyl acetate................                 141-78-6  1666/D3695/
                                                         624.1.
n-Heptane....................                 142-82-5  1666/D3695.
n-Hexane.....................                 110-54-3  1666/D3695.
Isobutyraldehyde.............                  78-84-2  1666/1667.
Isopropanol..................                  67-63-0  1666/D3695.
Isopropyl acetate............                 108-21-4  1666/D3695.
Isopropyl ether..............                 108-20-3  1666/D3695.
Methanol.....................                  67-56-1  1666/1671/D3695/
                                                         624.1.
Methyl Cellosolve [supreg] (2-                109-86-4  1666/1671.
 Methoxy ethanol).
Methylene chloride...........                  75-09-2  502.2/524.2/
                                                         624.1.
Methyl formate...............                 107-31-3  1666.
4-Methyl-2-pentanone (MIBK)..                 108-10-1  1624C/1666/D3695/
                                                         D4763/524.2/
                                                         624.1.
Phenol.......................                 108-95-2  D4763.
n-Propanol...................                  71-23-8  1666/1671/D3695/
                                                         624.1.
2-Propanone (Acetone)........                  67-64-1  D3695/D4763/
                                                         524.2/624.1.
Tetrahydrofuran..............                 109-99-9  1666/524.2/
                                                         624.1.
Toluene......................                 108-88-3  D3695/D4763/
                                                         502.2/524.2/
                                                         624.1.
Triethlyamine................                 121-44-8  1666/1671.
Xylenes......................                 (Note 1)  1624C/1666/
                                                         624.1.
------------------------------------------------------------------------
Table 1F note:
\1\ 1624C: m-xylene 108-38-3, o,p-xylene, E-14095 (Not a CAS number;
  this is the number provided in the Environmental Monitoring Methods
  Index [EMMI] database.); 1666: m,p-xylene 136777-61-2, o-xylene 95-47-
  6.

                    Table 1G--Test Methods for Pesticide Active Ingredients (40 CFR part 455)
----------------------------------------------------------------------------------------------------------------
                                                                                EPA analytical method
          EPA survey code                Pesticide name                 CAS No.                  No.(s) \3\
----------------------------------------------------------------------------------------------------------------
8..................................  Triadimefon...........                    43121-43-3  507/633/525.1/525.2/
                                                                                1656/625.1.
12.................................  Dichlorvos............                       62-73-7  1657/507/622/525.1/
                                                                                525.2/625.1.
16.................................  2,4-D; 2,4-D Salts and                       94-75-7  1658/515.1/615/515.2/
                                      Esters [2,4-Dichloro-                                 555.
                                      phenoxyacetic acid].
17.................................  2,4-DB; 2,4-DB Salts                         94-82-6  1658/515.1/615/515.2/
                                      and Esters [2,4-                                      555.
                                      Dichlorophenoxybutyri
                                      c acid].
22.................................  Mevinphos.............                     7786-34-7  1657/507/622/525.1/
                                                                                525.2/625.1.
25.................................  Cyanazine.............                    21725-46-2  629/507/608.3/625.1.
26.................................  Propachlor............                     1918-16-7  1656/508/608.1/525.1/
                                                                                525.2/608.3/625.1.
27.................................  MCPA; MCPA Salts and                         94-74-6  1658/615/555.
                                      Esters [2-Methyl-4-
                                      chlorophenoxyacetic
                                      acid].
30.................................  Dichlorprop;                                120-36-5  1658/515.1/615/515.2/
                                      Dichlorprop Salts and                                 555.
                                      Esters [2-(2,4-
                                      Dichlorophenoxy)
                                      propionic acid].
31.................................  MCPP; MCPP Salts and                         93-65-2  1658/615/555.
                                      Esters [2-(2-Methyl-4-
                                      chlorophenoxy)
                                      propionic acid].
35.................................  TCMTB [2-                                 21564-17-0  637.
                                      (Thiocyanomethylthio)
                                      benzo-thiazole].
39.................................  Pronamide.............                    23950-58-5  525.1/525.2/507/633.1/
                                                                                625.1.
41.................................  Propanil..............                      709-98-8  632.1/1656/608.3.
45.................................  Metribuzin............                    21087-64-9  507/633/525.1/525.2/
                                                                                1656/608.3/ 625.1.
52.................................  Acephate..............                    30560-19-1  1656/1657/608.3.
53.................................  Acifluorfen...........                    50594-66-6  515.1/515.2/555.
54.................................  Alachlor..............                    15972-60-8  505/507/645/525.1/
                                                                                525.2/1656/608.3/
                                                                                625.1.
55.................................  Aldicarb..............                      116-06-3  531.1.
58.................................  Ametryn...............                      834-12-8  507/619/525.2/625.1.
60.................................  Atrazine..............                     1912-24-9  505/507/619/525.1/
                                                                                525.2/1656/ 608.3/
                                                                                625.1.
62.................................  Benomyl...............                    17804-35-2  631.
68.................................  Bromacil; Bromacil                          314-40-9  507/633/525.1/525.2/
                                      Salts and Esters.                                     1656/608.3/ 625.1.
69.................................  Bromoxynil............                     1689-84-5  1625/1661/625.1.
69.................................  Bromoxynil octanoate..                     1689-99-2  1656/608.3.
70.................................  Butachlor.............                    23184-66-9  507/645/525.1/525.2/
                                                                                1656/608.3/625.1.
73.................................  Captafol..............                     2425-06-1  1656/608.3/625.1.
75.................................  Carbaryl [Sevin]......                       63-25-2  531.1/632/553/625.1.
76.................................  Carbofuran............                     1563-66-2  531.1/632/625.1.

80.................................  Chloroneb.............                     2675-77-6  1656/508/608.1/525.1/
                                                                                525.2/608.3/625.1.
82.................................  Chlorothalonil........                     1897-45-6  508/608.2/525.1/525.2/
                                                                                1656/608.3/625.1.
84.................................  Stirofos..............                      961-11-5  1657/507/622/525.1/
                                                                                525.2/625.1.
86.................................  Chlorpyrifos..........                     2921-88-2  1657/508/622/625.1.
90.................................  Fenvalerate...........                    51630-58-1  1660.
103................................  Diazinon..............                      333-41-5  1657/507/614/622/
                                                                                525.2/625.1.
107................................  Parathion methyl......                      298-00-0  1657/614/622/625.1.
110................................  DCPA [Dimethyl 2,3,5,6-                    1861-32-1  508/608.2/525.1/525.2/
                                      tetrachloro-                                          515.1 \2\/515.2 \2\/
                                      terephthalate].                                       1656/608.3/625.1.
112................................  Dinoseb...............                       88-85-7  1658/515.1/615/515.2/
                                                                                555/625.1.
113................................  Dioxathion............                       78-34-2  1657/614.1.
118................................  Nabonate [Disodium                          138-93-2  630.1.
                                      cyanodithio-
                                      imidocarbonate].
119................................  Diuron................                      330-54-1  632/553.
123................................  Endothall.............                      145-73-3  548/548.1.
124................................  Endrin................                       72-20-8  1656/505/508/617/
                                                                                525.1/525.2/608.3/
                                                                                625.1.
125................................  Ethalfluralin.........                    55283-68-6  1656/627/608.3 See
                                                                                footnote 1.
126................................  Ethion................                      563-12-2  1657/614/614.1/625.1.
127................................  Ethoprop..............                    13194-48-4  1657/507/622/525.1/
                                                                                525.2/625.1.
132................................  Fenarimol.............                    60168-88-9  507/633.1/525.1/525.2/
                                                                                1656/608.3/625.1.
133................................  Fenthion..............                       55-38-9  1657/622/625.1.
138................................  Glyphosate [N-                             1071-83-6  547.
                                      (Phosphonomethyl)
                                      glycine].
140................................  Heptachlor............                       76-44-8  1656/505/508/617/
                                                                                525.1/525.2/608.3/
                                                                                625.1.
144................................  Isopropalin...........                    33820-53-0  1656/627/608.3.
148................................  Linuron...............                      330-55-2  553/632.
150................................  Malathion.............                      121-75-5  1657/614/625.1.
154................................  Methamidophos.........                    10265-92-6  1657.
156................................  Methomyl..............                    16752-77-5  531.1/632.
158................................  Methoxychlor..........                       72-43-5  1656/505/508/608.2/
                                                                                617/525.1/525.2/
                                                                                608.3/625.1.
172................................  Nabam.................                      142-59-6  630/630.1.
173................................  Naled.................                      300-76-5  1657/622/625.1.
175................................  Norflurazon...........                    27314-13-2  507/645/525.1/525.2/
                                                                                1656/608.3/625.1.
178................................  Benfluralin...........                     1861-40-1  1656/627/608.3 See
                                                                                footnote 1.
182................................  Fensulfothion.........                      115-90-2  1657/622/625.1.
183................................  Disulfoton............                      298-04-4  1657/507/614/622/
                                                                                525.2/625.1.
185................................  Phosmet...............                      732-11-6  1657/622.1/625.1.
186................................  Azinphos Methyl.......                       86-50-0  1657/614/622/625.1.
192................................  Organo-tin pesticides.                    12379-54-3  Ind-01/200.7/200.9.
197................................  Bolstar...............                    35400-43-2  1657/622.
203................................  Parathion.............                       56-38-2  1657/614/625.1.
204................................  Pendimethalin.........                    40487-42-1  1656.
205................................  Pentachloronitrobenzen                       82-68-8  1656/608.1/617/608.3/
                                      e.                                                    625.1.
206................................  Pentachlorophenol.....                       87-86-5  1625/515.2/555/515.1/
                                                                                525.1/525.2/625.1.
208................................  Permethrin............                    52645-53-1  608.2/508/525.1/525.2/
                                                                                1656/1660/608.3 \4\/
                                                                                625.1 \4\.
212................................  Phorate...............                      298-02-2  1657/622/625.1.
218................................  Busan 85 [Potassium                         128-03-0  630/630.1.
                                      dimethyldithiocarbama
                                      te].
219................................  Busan 40 [Potassium N-                    51026-28-9  630/630.1.
                                      hydroxymethyl-N-
                                      methyldithiocarbamate
                                      ].
220................................  KN Methyl [Potassium N-                     137-41-7  630/630.1.
                                      methyl-
                                      dithiocarbamate].
223................................  Prometon..............                     1610-18-0  507/619/525.2/625.1.
224................................  Prometryn.............                     7287-19-6  507/619/525.1/525.2/
                                                                                625.1.
226................................  Propazine.............                      139-40-2  507/619/525.1/525.2/
                                                                                1656/608.3/625.1.
230................................  Pyrethrin I...........                      121-21-1  1660.
232................................  Pyrethrin II..........                      121-29-9  1660.
236................................  DEF [S,S,S-Tributyl                          78-48-8  1657.
                                      phosphorotrithioate].
239................................  Simazine..............                      122-34-9  505/507/619/525.1/
                                                                                525.2/1656/608.3/
                                                                                625.1.
241................................  Carbam-S [Sodium                            128-04-1  630/630.1.
                                      dimethyldithio-
                                      carbamate].
243................................  Vapam [Sodium                               137-42-8  630/630.1.
                                      methyldithiocarbamate
                                      ].
252................................  Tebuthiuron...........                    34014-18-1  507/525.1/525.2/
                                                                                625.1.
254................................  Terbacil..............                     5902-51-2  507/633/525.1/525.2/
                                                                                1656/608.3/625.1.
255................................  Terbufos..............                    13071-79-9  1657/507/614.1/525.1/
                                                                                525.2/625.1.
256................................  Terbuthylazine........                     5915-41-3  619/1656/608.3.
257................................  Terbutryn.............                      886-50-0  507/619/525.1/525.2/
                                                                                625.1.
259................................  Dazomet...............                      533-74-4  630/630.1/1659.
262................................  Toxaphene.............                     8001-35-2  1656/505/508/617/
                                                                                525.1/525.2/608.3/
                                                                                625.1.
263................................  Merphos [Tributyl                           150-50-5  1657/507/525.1/525.2/
                                      phosphorotrithioate].                                 622/625.1.
264................................  Trifluralin \1\.......                     1582-09-8  1656/508/617/627/
                                                                                525.2/608.3/625.1.

268................................  Ziram [Zinc                                 137-30-4  630/630.1.
                                      dimethyldithiocarbama
                                      te].
----------------------------------------------------------------------------------------------------------------
Table 1G notes:
\1\ Monitor and report as total Trifluralin.
\2\ Applicable to the analysis of DCPA degradates.
\3\ EPA Methods 608.1 through 645, 1645 through 1661, and Ind-01 are available in Methods for the Determination
  of Nonconventional Pesticides in Municipal and Industrial Wastewater, Volume I, EPA 821-R-93-010A, Revision I,
  August 1993, U.S. EPA. EPA Methods 200.9 and 505 through 555 are available in Methods for the Determination of
  Nonconventional Pesticides in Municipal and Industrial Wastewater, Volume II, EPA 821-R-93-010B, August 1993,
  U.S. EPA. The full text of Methods 608.3, 625.1, and 1625 are provided at Appendix A of this part 136. The
  full text of Method 200.7 is provided at Appendix C of this part 136. Methods 608.3 and 625.1 are available
  at: http://water.epa.gov/scitech/methods/cwa/methods_index.cfm (this is a placeholder for now).
\4\ Permethrin is not listed within methods 608.3 and 625.1; however, cis-permethrin and trans-permethrin are
  listed. Permethrin can be calculated by adding the results of cis and trans-permethrin.

                      Table 1H--List of Approved Microbiological Methods for Ambient Water
----------------------------------------------------------------------------------------------------------------
                                                                                AOAC,
      Parameter and units            Method \1\              EPA          Standard     ASTM,         Other
                                                                           methods     USGS
----------------------------------------------------------------------------------------------------------------
Bacteria:
----------------------------------------------------------------------------------------------------------------
1. Coliform (fecal), number per  Most Probable       p. 132 \3\........  9221 C E-
 100 mL or number per gram dry    Number (MPN), 5                         2006
 weight.                          tube, 3 dilution,
                                  or.
                                 Membrane filter     p. 124 \3\........  9222 D-     B-0050-8
                                  (MF),\2\ single                         2006 \27\   5 \4\
                                  step.
2. Coliform (fecal) in presence  MPN, 5 tube, 3      p. 132 \3\........  9221 C E-
 of chlorine, number per 100 mL.  dilution, or.                           2006
                                 MF \2\, single      p. 124 \3\........  9222 D-
                                  step \5\.                               2006 \27\
3. Coliform (total), number per  MPN, 5 tube, 3      p. 114 \3\........  9221 B-
 100 mL.                          dilution, or.                           2006
                                 MF \2\, single      p. 108 \3\........  9222 B-     B-0025-8
                                  step or two step.                       2006        5 \4\
4. Coliform (total), in          MPN, 5 tube, 3      p. 114 \3\........  9221 B-
 presence of chlorine, number     dilution, or.                           2006
 per 100 mL.
                                 MF \2\ with         p. 111 \3\........  9222 B-
                                  enrichment.                             2006
5.E. coli, number per 100 mL...  MPN,6 8 14          ..................  9221 B.2-
                                  multiple tube, or.                      2006/9221
                                                                          F-2006 11
                                                                          13
                                 Multiple tube/      ..................  9223 B-     991.15    Colilert[supreg]
                                  multiple well, or.                      2004 \12\   \10\      12 16, Colilert-
                                                                                [supreg] 12 15
                                                                                16
                                 MF 2 5 6 7 8, two   1103.1 \19\.......  9222 B-     D5392-93
                                  step, or.                               2006/9222   \9\
                                                                          G-2006,\1
                                                                          8\ 9213 D-
                                                                          2007
                                 Single step.......  1603 \20\, 1604     ..........  ........  mColiBlue-24[supr
                                                      \21\.                                     eg] \17\
6. Fecal streptococci, number    MPN, 5 tube, 3      p. 139 \3\........  9230 B-
 per 100 mL.                      dilution, or.                           2007
                                 MF \2\, or........  p. 136 \3\........  9230 C-     B-0055-8
                                                                          2007        5 \4\
                                 Plate count.......  p. 143 \3\........
7. Enterococci, number per 100   MPN,6 8 multiple    ..................  9230 D-     D6503-99  Ente-
 mL.                              tube/multiple                           2007        \9\       rolert[supreg]
                                  well, or.                                                     12 22
                                 MF 2 5 6 7 8 two    1106.1 \23\.......  9230 C-     D5259-92
                                  step, or.                               2007        \9\
                                 Single step, or...  1600 \24\.........  9230 C-
                                                                          2007
                                 Plate count.......  p. 143 \3\........
Protozoa:......................
8.Cryptosporidium..............  Filtration/IMS/FA.  1622 \25\, 1623
                                                      \26\.
9.Giardia......................  Filtration/IMS/FA.  1623 \26\.........
----------------------------------------------------------------------------------------------------------------
Table 1H notes:
\1\ The method must be specified when results are reported.
\2\ A 0.45-[micro]m membrane filter (MF) or other pore size certified by the manufacturer to fully retain
  organisms to be cultivated and to be free of extractables which could interfere with their growth.
\3\ Microbiological Methods for Monitoring the Environment, Water, and Wastes. EPA/600/8-78/017. 1978. US EPA.
\4\ U.S. Geological Survey Techniques of Water-Resource Investigations, Book 5, Laboratory Analysis, Chapter A4,
  Methods for Collection and Analysis of Aquatic Biological and Microbiological Samples. 1989. USGS.
\5\ Because the MF technique usually yields low and variable recovery from chlorinated wastewaters, the Most
  Probable Number method will be required to resolve any controversies.
\6\ Tests must be conducted to provide organism enumeration (density). Select the appropriate configuration of
  tubes/filtrations and dilutions/volumes to account for the quality, character, consistency, and anticipated
  organism density of the water sample.

\7\ When the MF method has not been used previously to test waters with high turbidity, large numbers of
  noncoliform bacteria, or samples that may contain organisms stressed by chlorine, a parallel test should be
  conducted with a multiple-tube technique to demonstrate applicability and comparability of results.
\8\ To assess the comparability of results obtained with individual methods, it is suggested that side-by-side
  tests be conducted across seasons of the year with the water samples routinely tested in accordance with the
  most current Standard Methods for the Examination of Water and Wastewater or EPA alternate test procedure
  (ATP) guidelines.
\9\ Annual Book of ASTM Standards--Water and Environmental Technology. Section 11.02. 2000, 1999, 1996. ASTM
  International.
\10\ Official Methods of Analysis of AOAC International, 16th Edition, Volume I, Chapter 17. 1995. AOAC
  International.
\11\ The multiple-tube fermentation test is used in 9221B.2-2006. Lactose broth may be used in lieu of lauryl
  tryptose broth (LTB), if at least 25 parallel tests are conducted between this broth and LTB using the water
  samples normally tested, and this comparison demonstrates that the false-positive rate and false-negative rate
  for total coliform using lactose broth is less than 10 percent. No requirement exists to run the completed
  phase on 10 percent of all total coliform-positive tubes on a seasonal basis.
\12\ These tests are collectively known as defined enzyme substrate tests, where, for example, a substrate is
  used to detect the enzyme [beta]-glucuronidase produced by E. coli.
\13\ After prior enrichment in a presumptive medium for total coliform using 9221B.2-2006, all presumptive tubes
  or bottles showing any amount of gas, growth or acidity within 48 h  3 h of incubation shall be
  submitted to 9221F-2006. Commercially available EC-MUG media or EC media supplemented in the laboratory with
  50 [micro]g/mL of MUG may be used.
\14\ Samples shall be enumerated by the multiple-tube or multiple-well procedure. Using multiple-tube
  procedures, employ an appropriate tube and dilution configuration of the sample as needed and report the Most
  Probable Number (MPN). Samples tested with Colilert[supreg] may be enumerated with the multiple-well
  procedures, Quanti-Tray[supreg] or Quanti-Tray[supreg]/2000, and the MPN calculated from the table provided by
  the manufacturer.
\15\ Colilert-18[supreg] is an optimized formulation of the Colilert[supreg] for the determination of total
  coliforms and E. coli that provides results within 18 h of incubation at 35 [deg]C, rather than the 24 h
  required for the Colilert[supreg] test, and is recommended for marine water samples.
\16\ Descriptions of the Colilert[supreg], Colilert-18[supreg], Quanti-Tray[supreg], and Quanti-Tray[supreg]/
  2000 may be obtained from IDEXX Laboratories Inc.
\17\ A description of the mColiBlue24[supreg] test may be obtained from Hach Company.
\18\ Subject total coliform positive samples determined by 9222B-1997 or other membrane filter procedure to
  9222G-1997 using NA-MUG media.
\19\ Method 1103.1: Escherichia coli (E. coli) in Water by Membrane Filtration Using membrane-Thermotolerant
  Escherichia coli Agar (mTEC), EPA-821-R-10-002. March 2010. US EPA.
\20\ Method 1603: Escherichia coli (E. coli) in Water by Membrane Filtration Using Modified membrane-
  Thermotolerant Escherichia coli Agar (Modified mTEC), EPA-821-R-14-010. September 2014. US EPA.
\21\ Preparation and use of MI agar with a standard membrane filter procedure is set forth in the article,
  Brenner et al. 1993. New Medium for the Simultaneous Detection of Total Coliform and Escherichia coli in
  Water. Appl. Environ. Microbiol. 59:3534-3544 and in Method 1604: Total Coliforms and Escherichia coli (E.
  coli) in Water by Membrane Filtration by Using a Simultaneous Detection Technique (MI Medium), EPA 821-R-02-
  024, September 2002, US EPA.
\22\ A description of the Enterolert[supreg] test may be obtained from IDEXX Laboratories Inc.
\23\ Method 1106.1: Enterococci in Water by Membrane Filtration Using membrane-Enterococcus-Esculin Iron Agar
  (mE-EIA), EPA-821-R-09-015. December 2009. US EPA.
\24\ Method 1600: Enterococci in Water by Membrane Filtration Using membrane-Enterococcus Indoxyl-[beta]-D-
  Glucoside Agar (mEI), EPA-821-R-14-011. September 2014. US EPA.
\25\ Method 1622 uses a filtration, concentration, immunomagnetic separation of oocysts from captured material,
  immunofluorescence assay to determine concentrations, and confirmation through vital dye staining and
  differential interference contrast microscopy for the detection of Cryptosporidium. Method 1622:
  Cryptosporidium in Water by Filtration/IMS/FA, EPA-821-R-05-001. December 2005. US EPA.
\26\ Method 1623 uses a filtration, concentration, immunomagnetic separation of oocysts and cysts from captured
  material, immunofluorescence assay to determine concentrations, and confirmation through vital dye staining
  and differential interference contrast microscopy for the simultaneous detection of Cryptosporidium and
  Giardia oocysts and cysts. Method 1623: Cryptosporidium and Giardia in Water by Filtration/IMS/FA. EPA-821-R-
  05-002. December 2005. US EPA.
\27\ The verification frequency is at least five typical and five atypical colonies per sampling site on the day
  of sample collection and analysis.

    (b) The documents required in this section are incorporated by
reference into this section in accordance with 5 U.S.C. 552(a) and 1
CFR part 51. Copies of the documents may be obtained from the sources
listed in paragraph (b) of this section. Documents may be inspected at
EPA's Water Docket, EPA West, 1301 Constitution Avenue NW., Room 3334,
Washington, DC 20004, (Telephone:<PHONE_NUMBER>); or at the National
Archives and Records Administration (NARA). For information on the
availability of this material at NARA, call<PHONE_NUMBER>, or go to:
http://www.archives.gov/federal_register/code_of_federal_regulations/ibr_locations.html. These test procedures are incorporated as they
exist on the day of approval and a notice of any change in these test
procedures will be published in the Federal Register. The full texts of
the methods from the following references which are cited in Tables IA,
IB, IC, ID, IE, IF, IG and IH of this section are incorporated by
reference into this regulation and may be obtained from the source
identified.
* * * * *
    (8) * * *
* * * * *
    (iv) Method 1600: Enterococci in Water by Membrane Filtration Using
membrane-Enterococcus Indoxyl-[beta]-D-Glucoside Agar (mEI). September
2014. EPA-821-R-14-011. Table IA, Note 25; Table IH, Note 24.
    (v) Method 1603: Escherichia coli (E. coli) in Water by Membrane
Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar
(Modified mTEC). September 2014. EPA-821-R-14-010. Table IA, Note 22;
Table IH, Note 20.
* * * * *
    (xiii) Method 1680: Fecal Coliforms in Sewage Sludge (Biosolids) by
Multiple-Tube Fermentation using Lauryl Tryptose Broth (LTB) and EC
Medium. September 2014. EPA-821-R-14-009. Table IA, Note 15.
* * * * *
    (xv) Method 1682: Salmonella in Sewage Sludge (Biosolids) by
Modified Semisolid Rappaport-Vassiliadis (MSRV) Medium. September 2014.
EPA 821-R-14-012. Table IA, Note 23.
* * * * *
    (10) * * *
* * * * *
    (viii) 2120, Color. 2011. Table IB.
* * * * *
    (x) 2310, Acidity. 2011. Table IB.
    (xi) 2320, Alkalinity. 2011. Table IB.
    (xii) 2340, Hardness. 2011. Table IB.
    (xiii) 2510, Conductivity. 2011. Table IB.
    (xiv) 2540, Solids. 2011. Table IB.
    (xv) 2550, Temperature. 2011. Table IB.
    (xvi) 3111, Metals by Flame Atomic Absorption Spectrometry. 2011.
Table IB.
    (xvii) 3112, Metals by Cold-Vapor Atomic Absorption Spectrometry.
2011. Table IB.
    (xviii) 3113, Metals by Electrothermal Atomic Absorption
Spectrometry. 2010. Table IB.
    (xix) 3114, Arsenic and Selenium by Hydride Generation/Atomic
Absorption Spectrometry. 2011. Table IB.

    (xx) 3120, Metals by Plasma Emission Spectroscopy. 2011. Table IB.
    (xxi) 3125, Metals by Inductively Coupled Plasma-Mass Spectrometry.
2011. Table IB.
    (xxii) 3500-Al, Aluminum. 2011. Table IB.
    (xxiii) 3500-As, Arsenic. 2011. Table IB.
    (xxiv) 3500-Ca, Calcium. 2011. Table IB.
    (xxv) 3500-Cr, Chromium. 2011. Table IB.
    (xxvi) 3500-Cu, Copper. 2011. Table IB.
    (xxvii) 3500-Fe, Iron. 2011. Table IB.
    (xxviii) 3500-Pb, Lead. 2011. Table IB.
    (xxix) 3500-Mn, Manganese. 2011. Table IB.
    (xxx) 3500-K, Potassium. 2011. Table IB.
    (xxxi) 3500-Na, Sodium. 2011. Table IB.
    (xxxii) 3500-V, Vanadium. 2011. Table IB.
    (xxxiii) 3500-Zn, Zinc. 2011. Table IB.
    (xxxiv) 4110, Determination of Anions by Ion Chromatography. 2011.
Table IB.
    (xxxv) 4140, Inorganic Anions by Capillary Ion Electrophoresis.
2011. Table IB.
    (xxxvi) 4500-B, Boron. 2011. Table IB.
    (xxxvii) 4500-Cl-, Chloride. 2011. Table IB.
    (xxxviii) 4500-Cl, Chlorine (Residual). 2011. Table IB.
    (xxxix) 4500-CN -, Cyanide. 2011. Table IB.
    (xl) 4500-F-, Fluoride. 2011. Table IB.
    (xli) 4500-H\+\, pH Value. 2011. Table IB.
    (xlii) 4500-NH3, Nitrogen (Ammonia). 2011. Table IB.
    (xliii) 4500-NO2-, Nitrogen (Nitrite). 2011.
Table IB.
    (xliv) 4500-NO3-, Nitrogen (Nitrate). 2011.
Table IB.
    (xlv) 4500-Norg, Nitrogen (Organic). 2011. Table IB.
    (xlvi) 4500-O, Oxygen (Dissolved). 2011. Table IB.
    (xlvii) 4500-P, Phosphorus. 2011. Table IB.
    (xlviii) 4500-SiO2, Silica. 2011. Table IB.
    (xlix) 4500-S\2\-, Sulfide. 2011. Table IB.
    (l) 4500-SO3\2\-, Sulfite. 2011. Table IB.
    (li) 4500-SO4\2\-, Sulfate. 2011. Table IB.
    (lii) 5210, Biochemical Oxygen Demand (BOD). 2011. Table IB.
    (liii) 5220, Chemical Oxygen Demand (COD). 2011. Table IB.
    (liv) 5310, Total Organic Carbon (TOC). 2011. Table IB.
    (lv) 5520, Oil and Grease. 2011. Table IB.
    (lvi) 5530, Phenols. 2010. Table IB.
    (lvii) 5540, Surfactants. 2011. Table IB.
    (lviii) 6200, Volatile Organic Compounds. 2011. Table IC.
* * * * *
    (lxi) 6440, Polynuclear Aromatic Hydrocarbons. 2005. Table IC.
    (lxii) 6630, Organochlorine Pesticides. 2007. Table ID.
    (lxiii) 6640, Acidic Herbicide Compounds. 2006. Table ID.
* * * * *
    (lxviii) 9222, Membrane Filter Technique for Members of the
Coliform Group. 2006. Table IA; Table IH, Note 18.
* * * * *
    (15) * * *
* * * * *
    (v) ASTM D511-09, Standard Test Methods for Calcium and Magnesium
in Water. May 2009. Table IB.
* * * * *
    (viii) ASTM D516-11, Standard Test Method for Sulfate Ion in Water,
September 2011. Table IB.
    (ix) ASTM D858-12, Standard Test Methods for Manganese in Water.
September 2012. Table IB.
    (x) ASTM D859-10, Standard Test Method for Silica in Water. July
2010. Table IB.
* * * * *
    (xii) ASTM D1067-11, Standard Test Methods for Acidity or
Alkalinity of Water. April 2011. Table IB.
    (xiii) ASTM D1068-10, Standard Test Methods for Iron in Water.
October 2010. Table IB.
* * * * *
    (xv) ASTM D1126-12, Standard Test Method for Hardness in Water.
March 2012. Table IB.
    (xvi) ASTM D1179-10, Standard Test Methods for Fluoride Ion in
Water. July 2010. Table IB.
    (xvii) ASTM D1246-10, Standard Test Method for Bromide Ion in
Water. July 2010. Table IB.
* * * * *
    (xxii) ASTM D1687-12 (Approved September 1, 2012), Standard Test
Methods for Chromium in Water. August 2007. Table IB.
    (xxiii) ASTM D1688-12, Standard Test Methods for Copper in Water.
September 2012. Table IB.
    (xxiv) ASTM D1691-12, Standard Test Methods for Zinc in Water.
September 2012. Table IB.
* * * * *
    (xxx) ASTM D1976-12, Standard Test Method for Elements in Water by
Inductively-Coupled Argon Plasma Atomic Emission Spectroscopy. March
2012. Table IB.
* * * * *
    (xxxv) ASTM D3223-12, Standard Test Method for Total Mercury in
Water. September 2012. Table IB.
* * * * *
    (xxxvii) ASTM D3373-12, Standard Test Method for Vanadium in Water.
September 2012. Table IB.
* * * * *
    (xxxix) ASTM D3557-12, Standard Test Method for Cadmium in Water.
September 2012. Table IB.
* * * * *
    (xlii) ASTM D3590-11, Standard Test Methods for Total Kjeldahl
Nitrogen in Water. April 2011. Table IB.
* * * * *
    (l) ASTM D4382-12, Standard Test Method for Barium in Water, Atomic
Absorption Spectrophotometry, Graphite Furnace. September 2012. Table
IB.
* * * * *
    (lii) ASTM D4658-09, Standard Test Method for Sulfide Ion in Water.
May 2009. Table IB.
* * * * *
    (lv) ASTM D5257-11, Standard Test Method for Dissolved Hexavalent
Chromium in Water by Ion Chromatography. April 2011. Table IB.
* * * * *
    (lviii) ASTM D5673-10, Standard Test Method for Elements in Water
by Inductively Coupled Plasma--Mass Spectrometry. September 2010. Table
IB.
    (lix) ASTM D5907-13, Standard Test Method for Filterable and
Nonfilterable Matter in Water. July 2013. Table IB.
* * * * *
    (lxi) ASTM. D6508-10, Standard Test Method for Determination of
Dissolved Inorganic Anions in Aqueous Matrices Using Capillary Ion
Electrophoresis and Chromate Electrolyte. October 2010. Table IB, Note
54.
* * * * *
    (lxvi) ASTM. D7284-13, Standard Test Method for Total Cyanide in
Water by Micro Distillation followed by Flow Injection Analysis with
Gas Diffusion Separation and Amperometric Detection. July 2013. Table
IB.
* * * * *
    (lxviii) ASTM. D7511-12, Standard Test Method for Total Cyanide by
Segmented Flow Injection Analysis, In-Line Ultraviolet Digestion and
Amperometric Detection. January 2012. Table IB.
* * * * *
    (19) * * *
* * * * *
    (vii) Method 10206, TNTplus 835-836 Nitrate Method,
Spectrophotometric Measurement of Nitrate in Water and

Wastewater. Revision 2.1, January 10, 2013. Table IB, Note 75.
    (viii) Method 10242, TNTplus 880 Total Kjeldahl Nitrogen Method,
Simplified Spectrophotometric Measurement of Total Kjeldahl Nitrogen in
Water and Wastewater. Revision 1.1, January 10, 2013. Table IB, Note
75.
* * * * *
    (20) * * *
    (i) Colilert[supreg]. 2013. Table IA, Notes 17 and 18; Table IH,
Notes 14, 15 and 16.
    (ii) Colilert-18[supreg]. 2013. Table IA, Notes 17 and 18; Table
IH, Notes 14, 15 and 16.
    (iii) Enterolert[supreg]. 2013. Table IA, Note 24; Table IH, Note
12.
    (iv) Quanti-Tray[supreg]. 2013. Table IA, Note 18; Table IH, Notes
14 and 16.
* * * * *
    (25) National Council of the Paper Industry for Air and Stream
Improvements, Inc. (NCASI), 260 Madison Avenue, New York NY 10016.
    (i) NCASI Methods TNTP-W10900 as an Alternative Testing Procedure
to EPA Method 351.2 and EPA Method 365.4. June 2011. Table IB, Note 77.
    (ii) NCASI Technical Bulletin No. 253, An Investigation of Improved
Procedures for Measurement of Mill Effluent and Receiving Water Color.
December 1971. Table IB, Note 18.
    (iii) NCASI Technical Bulletin No. 803, An Update of Procedures for
the Measurement of Color in Pulp Mill Wastewaters. May 2000. Table IB,
Note 18.
    (26) The Nitrate Elimination Co., Inc. (NECi), 334 Hecla St., Lake
Linden NI 49945.
    (i) NECi Method N07-0003, Method for Nitrate Reductase Nitrate-
Nitrogen Analysis. Revision 9.0. March 2014. Table IB, Note 73.
    (ii) [Reserved]
* * * * *
    (34) Timberline Instruments, LLC, 1880 South Flatiron Ct., Unit I,
Boulder CO 80301.
    (i) Determination of Inorganic Ammonia by Continuous Flow Gas
Diffusion and Conductivity Cell Analysis. June 24, 2011. Table IB, Note
74.
    (ii) [Reserved]
    (35) U.S. Geological Survey (USGS), U.S. Department of the
Interior, Reston, Virginia. Available from USGS Books and Open-File
Reports (OFR) Section, Federal Center, Box 25425, Denver, CO 80225.
    (i) Colorimetric determination of nitrate plus nitrite in water by
enzymatic reduction, automated discrete analyzer methods. U.S.
Geological Survey Techniques and Methods, Book 5, Chapter B8. 2011.
Table IB, Note 72.
    (ii) Methods for Determination of Inorganic Substances in Water and
Fluvial Sediments, editors, Techniques of Water-Resources
Investigations of the U.S. Geological Survey, Book 5, Chapter A1. 1979.
Table IB, Note 8.
    (iii) Methods for Determination of Inorganic Substances in Water
and Fluvial Sediments, Techniques of Water-Resources Investigations of
the U.S. Geological Survey, Book 5, Chapter A1. 1989. Table IB, Note 2.
    (iv) Methods for the Determination of Organic Substances in Water
and Fluvial Sediments. Techniques of Water-Resources Investigations of
the U.S. Geological Survey, Book 5, Chapter A3. 1987. Table IB, Note
24; Table ID, Note 4.
    (v) OFR 76-177, Selected Methods of the U.S. Geological Survey of
Analysis of Wastewaters. 1976. Table IE, Note 2.
    (vi) OFR 91-519, Methods of Analysis by the U.S. Geological Survey
National Water Quality Laboratory--Determination of Organonitrogen
Herbicides in Water by Solid-Phase Extraction and Capillary-Column Gas
Chromatography/Mass Spectrometry With Selected-Ion Monitoring. 1992.
Table ID, Note 14.
    (vii) OFR 92-146, Methods of Analysis by the U.S. Geological Survey
National Water Quality Laboratory--Determination of Total Phosphorus by
a Kjeldahl Digestion Method and an Automated Colorimetric Finish That
Includes Dialysis. 1992. Table IB, Note 48.
    (viii) OFR 93-125, Methods of Analysis by the U.S. Geological
Survey National Water Quality Laboratory--Determination of Inorganic
and Organic Constituents in Water and Fluvial Sediments. 1993. Table
IB, Note 51; Table IC, Note 9.
    (ix) OFR 93-449, Methods of Analysis by the U.S. Geological Survey
National Water Quality Laboratory--Determination of Chromium in Water
by Graphite Furnace Atomic Absorption Spectrophotometry. 1993. Table
IB, Note 46.
    (x) OFR 94-37, Methods of Analysis by the U.S. Geological Survey
National Water Quality Laboratory--Determination of Triazine and Other
Nitrogen-containing Compounds by Gas Chromatography with Nitrogen
Phosphorus Detectors. 1994. Table ID, Note 9.
    (xi) OFR 95-181, Methods of Analysis by the U.S. Geological Survey
National Water Quality Laboratory--Determination of Pesticides in Water
by C-18 Solid-Phase Extraction and Capillary-Column Gas Chromatography/
Mass Spectrometry With Selected-Ion Monitoring. 1995. Table ID, Note
11.
    (xii) OFR 97-198, Methods of Analysis by the U.S. Geological Survey
National Water Quality Laboratory--Determination of Molybdenum in Water
by Graphite Furnace Atomic Absorption Spectrophotometry. 1997. Table
IB, Note 47.
    (xiii) OFR 98-165, Methods of Analysis by the U.S. Geological
Survey National Water Quality Laboratory--Determination of Elements in
Whole-Water Digests Using Inductively Coupled Plasma-Optical Emission
Spectrometry and Inductively Coupled Plasma-Mass Spectrometry. 1998.
Table IB, Note 50.
    (xiv) OFR 98-639, Methods of Analysis by the U.S. Geological Survey
National Water Quality Laboratory--Determination of Arsenic and
Selenium in Water and Sediment by Graphite Furnace--Atomic Absorption
Spectrometry. 1999. Table IB, Note 49.
    (xv) OFR 00-170, Methods of Analysis by the U.S. Geological Survey
National Water Quality Laboratory--Determination of Ammonium Plus
Organic Nitrogen by a Kjeldahl Digestion Method and an Automated
Photometric Finish that Includes Digest Cleanup by Gas Diffusion. 2000.
Table IB, Note 45.
    (xvi) Techniques and Methods Book 5-B1, Determination of Elements
in Natural-Water, Biota, Sediment and Soil Samples Using Collision/
Reaction Cell Inductively Coupled Plasma-Mass Spectrometry. Chapter 1,
Section B, Methods of the National Water Quality Laboratory, Book 5,
Laboratory Analysis. 2006. Table IB, Note 70.
    (xvii) U.S. Geological Survey Techniques of Water-Resources
Investigations, Book 5, Laboratory Analysis, Chapter A4, Methods for
Collection and Analysis of Aquatic Biological and Microbiological
Samples. 1989. Table IA, Note 4; Table IH, Note 4.
    (xviii) Water-Resources Investigation Report 01-4098, Methods of
Analysis by the U.S. Geological Survey National Water Quality
Laboratory--Determination of Moderate-Use Pesticides and Selected
Degradates in Water by C-18 Solid-Phase Extraction and Gas
Chromatography/Mass Spectrometry. 2001. Table ID, Note 13.
    (xix) Water-Resources Investigations Report 01-4132, Methods of
Analysis by the U.S. Geological Survey National Water Quality
Laboratory--Determination of Organic Plus Inorganic Mercury in Filtered
and Unfiltered Natural Water With Cold Vapor-Atomic Fluorescence
Spectrometry. 2001. Table IB, Note 71.
    (xx) Water-Resources Investigation Report 01-4134, Methods of
Analysis by

the U.S. Geological Survey National Water Quality Laboratory--
Determination of Pesticides in Water by Graphitized Carbon-Based Solid-
Phase Extraction and High-Performance Liquid Chromatography/Mass
Spectrometry. 2001. Table ID, Note 12.
    (xxi) Water Temperature--Influential Factors, Field Measurement and
Data Presentation, Techniques of Water-Resources Investigations of the
U.S. Geological Survey, Book 1, Chapter D1. 1975. Table IB, Note 32.
* * * * *
    (c) Under certain circumstances, the Director may establish
limitations on the discharge of a parameter for which there is no test
procedure in this part or in 40 CFR parts 405 through 499. In these
instances the test procedure shall be specified by the Director.
* * * * *
    (e) * * *

                    Table II--Required Containers, Preservation Techniques, and Holding Times
----------------------------------------------------------------------------------------------------------------
                                                                                Maximum holding time
        Parameter number/name               Container \1\           Preservation 2 3               \4\
----------------------------------------------------------------------------------------------------------------
                                           Table IA--Bacterial Tests:
----------------------------------------------------------------------------------------------------------------
1-5. Coliform, total, fecal, and E.    PA, G..................  Cool, <10 [deg]C,        8 hours 22 23.
 coli.                                                           0.008% Na2S2O3\5\.
6. Fecal streptococci................  PA, G..................  Cool, <10 [deg]C,        8 hours \22\.
                                                                 0.008% Na2S2O3\5\.
7. Enterococci.......................  PA, G..................  Cool, <10 [deg]C,        8 hours \22\.
                                                                 0.008% Na2S2O3\5\.
8. Salmonella........................  PA, G..................  Cool, <10 [deg]C,        8 hours \22\.
                                                                 0.008% Na2S2O3\5\.
----------------------------------------------------------------------------------------------------------------
                                        Table IA--Aquatic Toxicity Tests:
----------------------------------------------------------------------------------------------------------------
9-12. Toxicity, acute and chronic....  P, FP, G...............  Cool, <=6 [deg]C \16\..  36 hours.
----------------------------------------------------------------------------------------------------------------
                                           Table IB--Inorganic Tests:
----------------------------------------------------------------------------------------------------------------
1. Acidity...........................  P, FP, G...............  Cool, <=6 [deg]C \18\..  14 days.
2. Alkalinity........................  P, FP, G...............  Cool, <=6 [deg]C \18\..  14 days.
4. Ammonia...........................  P, FP, G...............  Cool, <=6 [deg]C \18\,   28 days.
                                                                 H2SO4 to pH <2.
9. Biochemical oxygen demand.........  P, FP, G...............  Cool, <=6 [deg]C \18\..  48 hours.
10. Boron............................  P, FP, or Quartz.......  HNO3 to pH <2..........  6 months.
11. Bromide..........................  P, FP, G...............  None required..........  28 days.
14. Biochemical oxygen demand,         P, FP G................  Cool, <=6 [deg]C \18\..  48 hours.
 carbonaceous.
15. Chemical oxygen demand...........  P, FP, G...............  Cool, <=6 [deg]C \18\,   28 days.
                                                                 H2SO4 to pH <2.
16. Chloride.........................  P, FP, G...............  None required..........  28 days.
17. Chlorine, total residual.........  P, G...................  None required..........  Analyze within 15
                                                                                minutes.
21. Color............................  P, FP, G...............  Cool, <=6 [deg]C \18\..  48 hours.
23-24. Cyanide, total or available     P, FP, G...............  Cool, <=6 [deg]C \18\,   14 days.
 (or CATC) and free.                                             NaOH to pH >10 [shel5]
                                                                 [shel6], reducing
                                                                 agent if oxidizer
                                                                 present.
25. Fluoride.........................  P......................  None required..........  28 days.
27. Hardness.........................  P, FP, G...............  HNO3 or H2SO4 to pH <2.  6 months.
28. Hydrogen ion (pH)................  P, FP, G...............  None required..........  Analyze within 15
                                                                                minutes.
31, 43. Kjeldahl and organic N.......  P, FP, G...............  Cool, <=6 [deg]C \18\,   28 days.
                                                                 H2SO4 to pH <2.
----------------------------------------------------------------------------------------------------------------
                                              Table IB--Metals: \7\
----------------------------------------------------------------------------------------------------------------
18. Chromium VI......................  P, FP, G...............  Cool, <=6 [deg]C \18\,   28 days.
                                                                 pH = 9.3-9.7 \20\.
35. Mercury (CVAA)...................  P, FP, G...............  HNO3 to pH <2..........  28 days.
35. Mercury (CVAFS)..................  FP, G; and FP-lined cap  5 mL/L 12N HCl or 5 mL/  90 days \17\.
                                        \17\.                    L BrCl \17\.
3, 5-8, 12, 13, 19, 20, 22, 26, 29,    P, FP, G...............  HNO3 to pH <2, or at     6 months.
 30, 32-34, 36, 37, 45, 47, 51, 52,                              least 24 hours prior
 58-60, 62, 63, 70-72, 74, 75.                                   to analysis \19\.
 Metals, except boron, chromium VI,
 and mercury.
38. Nitrate..........................  P, FP, G...............  Cool, <=6 [deg]C \18\..  48 hours.
39. Nitrate-nitrite..................  P, FP, G...............  Cool, <=6 [deg]C \18\,   28 days.
                                                                 H2SO4 to pH <2.
40. Nitrite..........................  P, FP, G...............  Cool, <=6 [deg]C \18\..  48 hours.
41. Oil and grease...................  G......................  Cool to <=6 [deg]C       28 days.
                                                                 \18\, HCl or H2SO4 to
                                                                 pH <2.
42. Organic Carbon...................  P, FP, G...............  Cool to <=6 [deg]C       28 days.
                                                                 \18\, HCl, H2SO4, or
                                                                 H3PO4 to pH <2.
44. Orthophosphate...................  P, FP, G...............  Cool, to <=6 [deg]C      Filter within 15
                                                                 \18\ \24\.               minutes; Analyze
                                                                                within 48 hours.

46. Oxygen, Dissolved Probe..........  G, Bottle and top......  None required..........  Analyze within 15
                                                                                minutes.
47. Winkler..........................  G, Bottle and top......  Fix on site and store    8 hours.
                                                                 in dark.
48. Phenols..........................  G......................  Cool, <=6 [deg]C \18\,   28 days.
                                                                 H2SO4 to pH <2.
49. Phosphorous (elemental)..........  G......................  Cool, <=6 [deg]C \18\..  48 hours.
50. Phosphorous, total...............  P, FP, G...............  Cool, <=6 [deg]C \18\,   28 days.
                                                                 H2SO4 to pH <2.
53. Residue, total...................  P, FP, G...............  Cool, <=6 [deg]C \18\..  7 days.
54. Residue, Filterable..............  P, FP, G...............  Cool, <=6 [deg]C \18\..  7 days.
55. Residue, Nonfilterable (TSS).....  P, FP, G...............  Cool, <=6 [deg]C \18\..  7 days.
56. Residue, Settleable..............  P, FP, G...............  Cool, <=6 [deg]C \18\..  48 hours.
57. Residue, Volatile................  P, FP, G...............  Cool, <=6 [deg]C \18\..  7 days.
61. Silica...........................  P or Quartz............  Cool, <=6 [deg]C \18\..  28 days.
64. Specific conductance.............  P, FP, G...............  Cool, <=6 [deg]C \18\..  28 days.
65. Sulfate..........................  P, FP, G...............  Cool, <=6 [deg]C \18\..  28 days.
66. Sulfide..........................  P, FP, G...............  Cool, <=6 [deg]C \18\,   7 days.
                                                                 add zinc acetate plus
                                                                 sodium hydroxide to pH
                                                                 >9.
67. Sulfite..........................  P, FP, G...............  None required..........  Analyze within 15
                                                                                minutes.
68. Surfactants......................  P, FP, G...............  Cool, <=6 [deg]C \18\..  48 hours.
69. Temperature......................  P, FP, G...............  None required..........  Analyze.
73. Turbidity........................  P, FP, G...............  Cool, <=6 [deg]C \18\..  48 hours.
----------------------------------------------------------------------------------------------------------------
                                          Table IC--Organic Tests: \8\
----------------------------------------------------------------------------------------------------------------
13, 18-20, 22, 24-28, 34-37, 39-43,    G, FP-lined septum.....  Cool, <=6 [deg]C \18\,   14 days.
 45-47, 56, 76, 104, 105, 108-111,                               0.008% Na2S2O3\5\.
 113. Purgeable Halocarbons.
6, 57, 106. Purgeable aromatic         G, FP-lined septum.....  Cool, <=6 [deg]C \18\,   14 days \9\.
 hydrocarbons.                                                   0.008% Na2S2O3\5\, HCl
                                                                 to pH 2 \9\.
3, 4. Acrolein and acrylonitrile.....  G, FP-lined septum.....  Cool, <=6 [deg]C \18\,   14 days \10\.
                                                                 0.008% Na2S2O3, pH to
                                                                 4-5 \10\.
23, 30, 44, 49, 53, 77, 80, 81, 98,    G, FP-lined cap........  Cool, <=6 [deg]C \18\,   7 days until
 100, 112. Phenols \11\.                                         0.008% Na2S2O3.          extraction, 40 days
                                                                                after extraction.
7, 38. Benzidines \11\ \12\..........  G, FP-lined cap........  Cool, <=6 [deg]C \18\,   7 days until extraction
                                                                 0.008% Na2S2O3\5\.       \13\.
14, 17, 48, 50-52. Phthalate esters    G, FP-lined cap........  Cool, <=6 [deg]C \18\..  7 days until
 \11\.                                                                                extraction, 40 days
                                                                                after extraction.
82-84. Nitrosamines \11\ \14\........  G, FP-lined cap........  Cool, <=6 [deg]C \18\,   7 days until
                                                                 store in dark, 0.008%    extraction, 40 days
                                                                 Na2S2O3\5\.              after extraction.
88-94. PCBs \11\.....................  G, FP-lined cap........  Cool, <=6 [deg]C \18\..  1 year until
                                                                                extraction, 1 year
                                                                                after extraction.
54, 55, 75, 79. Nitroaromatics and     G, FP-lined cap........  Cool, <=6 [deg]C \18\,   7 days until
 isophorone \11\.                                                store in dark, 0.008%    extraction, 40 days
                                                                 Na2S2O3 \5\.             after extraction.
1, 2, 5, 8-12, 32, 33, 58, 59, 74,     G, FP-lined cap........  Cool, <=6 [deg]C \18\,   7 days until
 78, 99, 101. Polynuclear aromatic                               store in dark, 0.008%    extraction, 40 days
 hydrocarbons \11\.                                              Na2S2O3\5\.              after extraction.
15, 16, 21, 31, 87. Haloethers \11\..  G, FP-lined cap........  Cool, <=6 [deg]C \18\,   7 days until
                                                                 0.008% Na2S2O3\5\.       extraction, 40 days
                                                                                after extraction.
29, 35-37, 63-65, 107. Chlorinated     G, FP-lined cap........  Cool, <=6 [deg]C \18\..  7 days until
 hydrocarbons \11\.                                                                       extraction, 40 days
                                                                                after extraction.
60-62, 66-72, 85, 86, 95-97, 102,      G......................  See footnote 11........  See footnote 11.
 103. CDDs/CDFs \11\.
    Aqueous Samples: Field and Lab     G......................  Cool, <=6 [deg]C \18\,   1 year.
     Preservation.                                               0.008% Na2S2O3\5\, pH
                                                                 <9.
    Solids and Mixed-Phase Samples:    G......................  Cool, <=6 [deg]C \18\..  7 days.
     Field Preservation.
    Tissue Samples: Field              G......................  Cool, <=6 [deg]C \18\..  24 hours.
     Preservation.
    Solids, Mixed-Phase, and Tissue    G......................  Freeze, <= -10 [deg]C..  1 year.
     Samples: Lab Preservation.
114-118. Alkylated phenols...........  G......................  Cool, <6 [deg]C, H2SO4   28 days until
                                                                 to pH <2.                extraction, 40 days
                                                                                after extraction.
119. Adsorbable Organic Halides (AOX)  G......................  Cool, <6 [deg]C, 0.008%  Hold at least 3 days,
                                                                 Na2S2O3, HNO3 to pH <2.  but not more than 6
                                                                                months.
120. Chlorinated Phenolics...........  G, FP-lined cap........  Cool, <6 [deg]C, 0.008%  30 days until
                                                                 Na2S2O3, H2SO4 to pH     acetylation, 30 days
                                                                 <2.                      after acetylation.
----------------------------------------------------------------------------------------------------------------
                                           Table ID--Pesticides Tests:
----------------------------------------------------------------------------------------------------------------
1-70. Pesticides \11\................  G, FP-lined cap........  Cool, <=6 [deg]C \18\,   7 days until
                                                                 pH 5-9 \15\.             extraction, 40 days
                                                                                after extraction.
----------------------------------------------------------------------------------------------------------------

                                          Table IE--Radiological Tests:
----------------------------------------------------------------------------------------------------------------
1-5. Alpha, beta, and radium.........  P, FP, G...............  HNO3 to pH <2..........  6 months.
----------------------------------------------------------------------------------------------------------------
                                           Table IH--Bacterial Tests:
----------------------------------------------------------------------------------------------------------------
1-4. Coliform, total, fecal..........  PA, G..................  Cool, <10 [deg]C,        8 hours \22\ \23\.
                                                                 0.008% Na2S2O3\5\.
5. E. coli...........................  PA, G..................  Cool, <10 [deg]C,        8 hours \22\.
                                                                 0.008% Na2S2O3\5\.
6. Fecal streptococci................  PA, G..................  Cool, <10 [deg]C,        8 hours \22\.
                                                                 0.008% Na2S2O3\5\.
7. Enterococci.......................  PA, G..................  Cool, <10 [deg]C,        8 hours \22\.
                                                                 0.008% Na2S2O3\5\.
----------------------------------------------------------------------------------------------------------------
                                           Table IH--Protozoan Tests:
----------------------------------------------------------------------------------------------------------------
8. Cryptosporidium...................  LDPE; field filtration.  1-10 [deg]C............  96 hours \21\.
9. Giardia...........................  LDPE; field filtration.  1-10 [deg]C............  96 hours \21\.
----------------------------------------------------------------------------------------------------------------
\1\ ``P'' is for polyethylene; ``FP'' is fluoropolymer (polytetrafluoroethylene (PTFE); Teflon[supreg]), or
  other fluoropolymer, unless stated otherwise in this Table II; ``G'' is glass; ``PA'' is any plastic that is
  made of a sterilizable material (polypropylene or other autoclavable plastic); ``LDPE'' is low density
  polyethylene.
\2\ Except where noted in this Table II and the method for the parameter, preserve each grab sample within 15
  minutes of collection. For a composite sample collected with an automated sample (e.g., using a 24-hour
  composite sample; see 40 CFR 122.21(g)(7)(i) or 40 CFR part 403, appendix E), refrigerate the sample at <= 6
  [deg]C during collection unless specified otherwise in this Table II or in the method(s). For a composite
  sample to be split into separate aliquots for preservation and/or analysis, maintain the sample at <= 6
  [deg]C, unless specified otherwise in this Table II or in the method(s), until collection, splitting, and
  preservation is completed. Add the preservative to the sample container prior to sample collection when the
  preservative will not compromise the integrity of a grab sample, a composite sample, or aliquot split from a
  composite sample within 15 minutes of collection. If a composite measurement is required but a composite
  sample would compromise sample integrity, individual grab samples must be collected at prescribed time
  intervals (e.g., 4 samples over the course of a day, at 6-hour intervals). Grab samples must be analyzed
  separately and the concentrations averaged. Alternatively, grab samples may be collected in the field and
  composited in the laboratory if the compositing procedure produces results equivalent to results produced by
  arithmetic averaging of results of analysis of individual grab samples. For examples of laboratory compositing
  procedures, see EPA Method 1664 Rev. A (oil and grease) and the procedures at 40 CFR 141.24(f)(14)(iv) and (v)
  (volatile organics).
\3\ When any sample is to be shipped by common carrier or sent via the U.S. Postal Service, it must comply with
  the Department of Transportation Hazardous Materials Regulations (49 CFR part 172). The person offering such
  material for transportation is responsible for ensuring such compliance. For the preservation requirement of
  Table II, the Office of Hazardous Materials, Materials Transportation Bureau, Department of Transportation has
  determined that the Hazardous Materials Regulations do not apply to the following materials: Hydrochloric acid
  (HCl) in water solutions at concentrations of 0.04% by weight or less (pH about 1.96 or greater; Nitric acid
  (HNO3) in water solutions at concentrations of 0.15% by weight or less (pH about 1.62 or greater); Sulfuric
  acid (H2SO4) in water solutions at concentrations of 0.35% by weight or less (pH about 1.15 or greater); and
  Sodium hydroxide (NaOH) in water solutions at concentrations of 0.080% by weight or less (pH about 12.30 or
  less).
\4\ Samples should be analyzed as soon as possible after collection. The times listed are the maximum times that
  samples may be held before the start of analysis and still be considered valid. Samples may be held for longer
  periods only if the permittee or monitoring laboratory have data on file to show that, for the specific types
  of samples under study, the analytes are stable for the longer time, and has received a variance from the
  Regional ATP Coordinator under Sec.   136.3(e). For a grab sample, the holding time begins at the time of
  collection. For a composite sample collected with an automated sampler (e.g., using a 24-hour composite
  sampler; see 40 CFR 122.21(g)(7)(i) or 40 CFR part 403, appendix E), the holding time begins at the time of
  the end of collection of the composite sample. For a set of grab samples composited in the field or
  laboratory, the holding time begins at the time of collection of the last grab sample in the set. Some samples
  may not be stable for the maximum time period given in the table. A permittee or monitoring laboratory is
  obligated to hold the sample for a shorter time if it knows that a shorter time is necessary to maintain
  sample stability. See Sec.   136.3(e) for details. The date and time of collection of an individual grab
  sample is the date and time at which the sample is collected. For a set of grab samples to be composited, and
  that are all collected on the same calendar date, the date of collection is the date on which the samples are
  collected. For a set of grab samples to be composited, and that are collected across two calendar dates, the
  date of collection is the dates of the two days; e.g., November 14-15. For a composite sample collected
  automatically on a given date, the date of collection is the date on which the sample is collected. For a
  composite sample collected automatically, and that is collected across two calendar dates, the date of
  collection is the dates of the two days; e.g., November 14-15. For static-renewal toxicity tests, each grab or
  composite sample may also be used to prepare test solutions for renewal at 24 h, 48 h, and/or 72 h after first
  use, if stored at 0-6 [deg]C, with minimum head space.
\5\ ASTM D7365-09a specifies treatment options for samples containing oxidants (e.g., chlorine) for cyanide
  analyses. Also, Section 9060A of Standard Methods for the Examination of Water and Wastewater (20th and 21st
  editions) addresses dechlorination procedures for microbiological analyses.
\6\ Sampling, preservation and mitigating interferences in water samples for analysis of cyanide are described
  in ASTM D7365-09a. There may be interferences that are not mitigated by the analytical test methods or D7365-
  09a. Any technique for removal or suppression of interference may be employed, provided the laboratory
  demonstrates that it more accurately measures cyanide through quality control measures described in the
  analytical test method. Any removal or suppression technique not described in D7365-09a or the analytical test
  method must be documented along with supporting data.
\7\ For dissolved metals, filter grab samples within 15 minutes of collection and before adding preservatives.
  For a composite sample collected with an automated sampler (e.g., using a 24-hour composite sampler; see 40
  CFR 122.21(g)(7)(i) or 40 CFR part 403, appendix E), filter the sample within 15 minutes after completion of
  collection and before adding preservatives. If it is known or suspected that dissolved sample integrity will
  be compromised during collection of a composite sample collected automatically over time (e.g., by interchange
  of a metal between dissolved and suspended forms), collect and filter grab samples to be composited (footnote
  2) in place of a composite sample collected automatically.
\8\ Guidance applies to samples to be analyzed by GC, LC, or GC/MS for specific compounds.
\9\ If the sample is not adjusted to pH 2, then the sample must be analyzed within seven days of sampling.
\10\ The pH adjustment is not required if acrolein will not be measured. Samples for acrolein receiving no pH
  adjustment must be analyzed within 3 days of sampling.

\11\ When the extractable analytes of concern fall within a single chemical category, the specified preservative
  and maximum holding times should be observed for optimum safeguard of sample integrity (i.e., use all
  necessary preservatives and hold for the shortest time listed). When the analytes of concern fall within two
  or more chemical categories, the sample may be preserved by cooling to <= 6 [deg]C, reducing residual chlorine
  with 0.008% sodium thiosulfate, storing in the dark, and adjusting the pH to 6-9; samples preserved in this
  manner may be held for seven days before extraction and for forty days after extraction. Exceptions to this
  optional preservation and holding time procedure are noted in footnote 5 (regarding the requirement for
  thiosulfate reduction), and footnotes 12, 13 (regarding the analysis of benzidine).
\12\ If 1,2-diphenylhydrazine is likely to be present, adjust the pH of the sample to 4.0  0.2 to
  prevent rearrangement to benzidine.
\13\ Extracts may be stored up to 30 days at < 0 [deg]C.
\14\ For the analysis of diphenylnitrosamine, add 0.008% Na2S2O3 and adjust pH to 7-10 with NaOH within 24 hours
  of sampling.
\15\ The pH adjustment may be performed upon receipt at the laboratory and may be omitted if the samples are
  extracted within 72 hours of collection. For the analysis of aldrin, add 0.008% Na2S2O3.
\16\ Place sufficient ice with the samples in the shipping container to ensure that ice is still present when
  the samples arrive at the laboratory. However, even if ice is present when the samples arrive, immediately
  measure the temperature of the samples and confirm that the preservation temperature maximum has not been
  exceeded. In the isolated cases where it can be documented that this holding temperature cannot be met, the
  permittee can be given the option of on-site testing or can request a variance. The request for a variance
  should include supportive data which show that the toxicity of the effluent samples is not reduced because of
  the increased holding temperature. Aqueous samples must not be frozen. Hand-delivered samples used on the day
  of collection do not need to be cooled to 0 to 6 [deg]C prior to test initiation.
\17\ Samples collected for the determination of trace level mercury (<100 ng/L) using EPA Method 1631 must be
  collected in tightly-capped fluoropolymer or glass bottles and preserved with BrCl or HCl solution within 48
  hours of sample collection. The time to preservation may be extended to 28 days if a sample is oxidized in the
  sample bottle. A sample collected for dissolved trace level mercury should be filtered in the laboratory
  within 24 hours of the time of collection. However, if circumstances preclude overnight shipment, the sample
  should be filtered in a designated clean area in the field in accordance with procedures given in Method 1669.
  If sample integrity will not be maintained by shipment to and filtration in the laboratory, the sample must be
  filtered in a designated clean area in the field within the time period necessary to maintain sample
  integrity. A sample that has been collected for determination of total or dissolved trace level mercury must
  be analyzed within 90 days of sample collection.
\18\ Aqueous samples must be preserved at <= 6 [deg]C, and should not be frozen unless data demonstrating that
  sample freezing does not adversely impact sample integrity is maintained on file and accepted as valid by the
  regulatory authority. Also, for purposes of NPDES monitoring, the specification of ``<= [deg]C'' is used in
  place of the ``4 [deg]C'' and ``< 4 [deg]C'' sample temperature requirements listed in some methods. It is not
  necessary to measure the sample temperature to three significant figures (1/100th of 1 degree); rather, three
  significant figures are specified so that rounding down to 6 [deg]C may not be used to meet the <=6 [deg]C
  requirement. The preservation temperature does not apply to samples that are analyzed immediately (less than
  15 minutes).
\19\ An aqueous sample may be collected and shipped without acid preservation. However, acid must be added at
  least 24 hours before analysis to dissolve any metals that adsorb to the container walls. If the sample must
  be analyzed within 24 hours of collection, add the acid immediately (see footnote 2). Soil and sediment
  samples do not need to be preserved with acid. The allowances in this footnote supersede the preservation and
  holding time requirements in the approved metals methods.
\20\ To achieve the 28-day holding time, use the ammonium sulfate buffer solution specified in EPA Method 218.6.
  The allowance in this footnote supersedes preservation and holding time requirements in the approved
  hexavalent chromium methods, unless this supersession would compromise the measurement, in which case
  requirements in the method must be followed.
\21\ Holding time is calculated from time of sample collection to elution for samples shipped to the laboratory
  in bulk and calculated from the time of sample filtration to elution for samples filtered in the field.
\22\ Sample analysis should begin as soon as possible after receipt; sample incubation must be started no later
  than 8 hours from time of collection.
\23\ For fecal coliform samples for sewage sludge (biosolids) only, the holding time is extended to 24 hours for
  the following sample types using either EPA Method 1680 (LTB-EC) or 1681 (A-1): Class A composted, Class B
  aerobically digested, and Class B anaerobically digested.
\24\ The immediate filtration requirement in orthophosphate measurement is to assess the dissolved or bio-
  available form of orthophosphorus (i.e., that which passes through a 0.45-micron filter), hence the
  requirement to filter the sample immediately upon collection (i.e., within 15 minutes of collection).

0
5. Section 136.4 is amended by revising paragraphs (a) introductory
text, (b), and (c) to read as follows:

Sec.  136.4  Application for and approval of alternate test procedures
for nationwide use.

    (a) A written application for review of an alternate test procedure
(alternate method) for nationwide use may be made by letter via email
or by hard copy in triplicate to the National Alternate Test Procedure
(ATP) Program Coordinator (National Coordinator), Office of Science and
Technology (4303T), Office of Water, U.S. Environmental Protection
Agency, 1200 Pennsylvania Ave. NW., Washington, DC 20460. Any
application for an ATP under this paragraph (a) shall:
* * * * *
    (b) The National Coordinator may request additional information and
analyses from the applicant in order to evaluate whether the alternate
test procedure satisfies the applicable requirements of this part.
    (c) Approval for nationwide use. (1) After a review of the
application and any additional analyses requested from the applicant,
the National Coordinator will notify the applicant, in writing, of
whether the National Coordinator will recommend approval or disapproval
of the alternate test procedure for nationwide use in CWA programs. If
the application is not recommended for approval, the National
Coordinator may specify what additional information might lead to a
reconsideration of the application and notify the Regional Alternate
Test Procedure Coordinators of the disapproval recommendation. Based on
the National Coordinator's recommended disapproval of a proposed
alternate test procedure and an assessment of any current approvals for
limited uses for the unapproved method, the Regional ATP Coordinator
may decide to withdraw approval of the method for limited use in the
Region.
    (2) Where the National Coordinator has recommended approval of an
applicant's request for nationwide use of an alternate test procedure,
the National Coordinator will notify the applicant. The National
Coordinator will also notify the Regional ATP Coordinators that they
may consider approval of this alternate test procedure for limited use
in their Regions based on the information and data provided in the
application until the alternate test procedure is approved by
publication in a final rule in the Federal Register.
    (3) EPA will propose to amend this part to include the alternate
test procedure in Sec.  136.3. EPA shall make available for review all
the factual bases for its proposal, including the method, any
performance data submitted by the applicant and any available EPA
analysis of those data.
    (4) Following public comment, EPA shall publish in the Federal
Register a final decision on whether to amend this part to include the
alternate test procedure as an approved analytical method for
nationwide use.
    (5) Whenever the National Coordinator has recommended approval of
an applicant's ATP request for nationwide use, any person may request
an approval of the method for limited use under Sec.  136.5 from the
EPA Region.

0
6. Section 136.5 is amended by revising paragraphs (a), (b), (c)(1),
and (d) to read as follows:

Sec.  136.5  Approval of alternate test procedures for limited use.

    (a) Any person may request the Regional ATP Coordinator to approve
the use of an alternate test procedure in the Region.
    (b) When the request for the use of an alternate test procedure
concerns use in a State with an NPDES permit program approved pursuant
to section 402 of the Act, the requestor shall first submit an
application for limited use to the Director of the State agency having
responsibility for issuance of NPDES permits within such State (i.e.,
permitting authority). The Director will forward the application to the
Regional ATP Coordinator with a recommendation for or against approval.
    (c) * * *
    (1) Provide the name and address of the applicant and the
applicable ID number of the existing or pending permit(s) and issuing
agency for which use of the alternate test procedure is requested, and
the discharge serial number.
* * * * *
    (d) Approval for limited use. (1) The Regional ATP Coordinator will
review the application and notify the applicant and the appropriate
State agency of approval or rejection of the use of the alternate test
procedure. The approval may be restricted to use only with respect to a
specific discharge or facility (and its laboratory) or, at the
discretion of the Regional ATP Coordinator, to all dischargers or
facilities (and their associated laboratories) specified in the
approval for the Region. If the application is not approved, the
Regional ATP Coordinator shall specify what additional information
might lead to a reconsideration of the application.
    (2) The Regional ATP Coordinator will forward a copy of every
approval and rejection notification to the National Alternate Test
Procedure Coordinator.
0
7. In Sec.  136.6:
0
a. Revise paragraphs (b)(1) and (2) introductory text.
0
b. Remove paragraph (b)(4)(xvi).
0
c. Redesignate paragraphs (b)(4)(xvii) through (xxii) as paragraphs
(b)(4)(xvi) through (xxi), respectively.
0
d. Add paragraph (c).
    The revision and addition read as follows:

Sec.  136.6  Method modifications and analytical requirements.

* * * * *
    (b) Method modifications. (1) If the underlying chemistry and
determinative technique in a modified method are essentially the same
as an approved part 136 method, then the modified method is an
equivalent and acceptable alternative to the approved method provided
the requirements of this section are met. However, those who develop or
use a modification to an approved (part 136) method must document that
the performance of the modified method, in the matrix to which the
modified method will be applied, is equivalent to the performance of
the approved method. If such a demonstration cannot be made and
documented, then the modified method is not an acceptable alternative
to the approved method. Supporting documentation must, if applicable,
include the routine initial demonstration of capability and ongoing QC
including determination of precision and accuracy, detection limits,
and matrix spike recoveries. Initial demonstration of capability
typically includes analysis of four replicates of a mid-level standard
and a method detection limit study. Ongoing quality control typically
includes method blanks, mid-level laboratory control samples, and
matrix spikes (QC is as specified in the method). The method is
considered equivalent if the quality control requirements in the
reference method are achieved. The method user's Standard Operating
Procedure (SOP) must clearly document the modifications made to the
reference method. Examples of allowed method modifications are listed
in this section. If the method user is uncertain whether a method
modification is allowed, the Regional ATP Coordinator or Director
should be contacted for approval prior to implementing the
modification. The method user should also complete necessary
performance checks to verify that acceptable performance is achieved
with the method modification prior to analyses of compliance samples.
    (2) Requirements. The modified method must meet or exceed
performance of the approved method(s) for the analyte(s) of interest,
as documented by meeting the initial and ongoing quality control
requirements in the method.
* * * * *
    (c) The permittee must notify their permitting authority of the
intent to use a modified method. Such notification should be of the
form ``Method xxx has been modified within the flexibility allowed in
40 CFR 136.6.'' The permittee may indicate the specific paragraph of
Sec.  136.6 allowing the method modification. Specific details of the
modification need not be provided, but must be documented in the
Standard Operating Procedure (SOP) and maintained by the analytical
laboratory that performs the analysis.
0
8. In Appendix A to part 136:
0
a. Revise Method 608.
0
b. Revise Method 611, section 1.1.
0
c. Revise Method 624.
0
d. Revise Method 625.
    The revisions read as follows:

Appendix A to Part 136--Methods for Organic Chemical Analysis of
Municipal and Industrial Wastewater

* * * * *

Method 608.3--Organochlorine Pesticides And PCBs By GC/HSD

1. Scope and Application

    1.1 This method is for determination of organochlorine pesticides
and polychlorinated biphenyls (PCBs) in industrial discharges and other
environmental samples by gas chromatography (GC) combined with a
halogen-specific detector (HSD; e.g., electron capture, electrolytic
conductivity), as provided under 40 CFR 136.1. This revision is based
on a previous protocol (Reference 1), on the revision promulgated
October 26, 1984 (49 FR 43234), on an inter-laboratory method
validation study (Reference 2), and on EPA Method 1656 (Reference 16).
The analytes that may be qualitatively and quantitatively determined
using this method and their CAS Registry numbers are listed in Table 1.
    1.2 This method may be extended to determine the analytes listed in
Table 2. However, extraction or gas chromatography challenges for some
of these analytes may make quantitative determination difficult.
    1.3 When this method is used to analyze unfamiliar samples for an
analyte listed in Table 1 or Table 2, analyte identification must be
supported by at least one additional qualitative technique. This method
gives analytical conditions for a second GC column that can be used to
confirm and quantify measurements.
    Additionally, Method 625 provides gas chromatograph/mass
spectrometer (GC/MS) conditions appropriate for the qualitative
confirmation of results for the analytes listed in Tables 1 and 2 using
the extract produced by this method, and Method 1699 (Reference 18)
provides high resolution GC/MS conditions for qualitative confirmation
of results using the original sample. When such methods are used to
confirm the identifications of the target analytes, the quantitative
results should be derived from the procedure with the

calibration range and sensitivity that are most appropriate for the
intended application.
    1.4 The large number of analytes in Tables 1 and 2 makes testing
difficult if all analytes are determined simultaneously. Therefore, it
is necessary to determine and perform quality control (QC) tests for
the ``analytes of interest'' only. The analytes of interest are those
required to be determined by a regulatory/control authority or in a
permit, or by a client. If a list of analytes is not specified, the
analytes in Table 1 must be determined, at a minimum, and QC testing
must be performed for these analytes. The analytes in Table 1 and some
of the analytes in Table 2 have been identified as Toxic Pollutants (40
CFR 401.15), expanded to a list of Priority Pollutants (40 CFR part
423, appendix A).
    1.5 In this revision to Method 608, Chlordane has been listed as
the alpha- and gamma-isomers in Table 1. Reporting may be by the
individual isomers, or as the sum of the concentrations of these
isomers, as requested or required by a regulatory/control authority or
in a permit. Technical Chlordane is listed in Table 2 and may be used
in cases where historical reporting has only been the Technical
Chlordane. Toxaphene and the PCBs have been moved from Table 1 to Table
2 (Additional Analytes) to distinguish these analytes from the analytes
required in quality control tests (Table 1). QC acceptance criteria for
Toxaphene and the PCBs have been retained in Table 4 and may continue
to be applied if desired, or if these analytes are requested or
required by a regulatory/control authority or in a permit. Method 1668C
(Reference 17) may be useful for determination of PCBs as individual
chlorinated biphenyl congeners, and Method 1699 (Reference 18) may be
useful for determination of the pesticides listed in this method.
However, at the time of writing of this revision, Methods 1668C and
1699 had not been approved for use at 40 CFR part 136.
    1.6 Method detection limits (MDLs; Reference 3) for the analytes in
Tables 1 and some of the analytes in Table 2 are listed in those
tables. These MDLs were determined in reagent water (Reference 3).
Advances in analytical technology, particularly the use of capillary
(open-tubular) columns, allowed laboratories to routinely achieve MDLs
for the analytes in this method that are 2-10 times lower than those in
the version promulgated in 1984 (40 FR 43234). The MDL for an analyte
in a specific wastewater may differ from those listed, depending upon
the nature of interferences in the sample matrix.