[Federal Register: March 14, 2008 (Volume 73, Number 51)]
[Notices]
[Page 13939-13940]
[DOCID:fr14mr08-105]

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SECURITIES AND EXCHANGE COMMISSION

[Release No. 34-57459; File No. SR-BSE-2008-13]

Self-Regulatory Organizations; Boston Stock Exchange, Inc.;
Notice of Filing and Immediate Effectiveness of Proposed Rule Change
Relating to the Fee Schedule of the Boston Options Exchange

March 10, 2008.
    Pursuant to Section 19(b)(1) of the Securities Exchange Act of 1934
(the ``Act''),\1\ and Rule 19b-4 thereunder,\2\ notice is hereby given
that on March 7, 2008, the Boston Stock Exchange, Inc. (``BSE'' or
``Exchange'') filed with the Securities and Exchange Commission
(``Commission'') the proposed rule change as described in Items I, II,
and III below, which Items have been substantially prepared by the BSE.
The BSE has designated the proposed rule change as constituting a
stated policy, practice, or interpretation with respect to the meaning,
administration, or enforcement of an existing rule of the self-
regulatory organization pursuant to Section 19(b)(3)(A)(i) of the Act
\3\ and Rule 19b-4(f)(1) thereunder,\4\ which renders the proposal
effective upon filing with the Commission. The Commission is publishing
this notice to solicit comments on the proposed rule change from
interested persons.
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    \1\ 15 U.S.C. 78s(b)(1).
    \2\ 17 CFR 240.19b-4.
    \3\ 15 U.S.C. 78s(b)(3)(A)(i).
    \4\ 17 CFR 240.19b-4(f)(1).
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I. Self-Regulatory Organization's Statement of the Terms of Substance
of the Proposed Rule Change

    The Exchange is proposing to amend the Fee Schedule of the Boston
Options Exchange (``BOX''). The proposed rule change will more clearly
set forth the fees that are presently already charged for trading
options contracts on BOX. The text of the proposed rule change is
available on BSE's Web site at http://www.bostonoptions.com, at BSE's
principal office, and at the Commission's Public Reference Room.

II. Self-Regulatory Organization's Statement of the Purpose of, and
Statutory Basis for, the Proposed Rule Change

    In its filing with the Commission, the BSE included statements
concerning the purpose of, and basis for, the proposed rule change and
discussed any comments it received on the proposed rule change. The
text of these statements may be examined at the places specified in
Item IV below. The BSE has prepared summaries, set forth in Sections A,
B, and C below, of the most significant aspects of such statements.

A. Self-Regulatory Organization's Statement of the Purpose of, and
Statutory Basis for, the Proposed Rule Change

1. Purpose
    The Exchange proposes to amend the BOX Fee Schedule. The proposed
change will more clearly set forth the fees that are applicable to
Public Customer and Broker Dealer account types. The proposed change
will not modify the level of fees that are charged for the trading of
options contracts on BOX, nor will it change to whom the fees are
charged.
    The Exchange currently applies an alternative pricing structure for
certain classes of options contracts traded on BOX, the Liquidity Make
or Take pricing structure (``Make or Take''). Make or Take fees and
credits apply to transactions for all account types (e.g., Public
Customer, Broker Dealer or Market Maker) as set forth in Section 7 of
the BOX Fee Schedule. Specific references to the Liquidity Make or Take
pricing structure and the fees and credits associated therewith are not
currently included within the sections of fees applicable to Public
Customer accounts as well as Broker Dealer proprietary accounts.
However, particular reference to Make or Take is made within the
section of fees

applicable to Market Makers. As previously stated, Make or Take is
applicable to all three account types.\5\
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    \5\ See Securities Exchange Act Release No. 56371 (September 7,
2007), 72 FR 52401 (September 13, 2007).
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    This proposed rule change will specifically include this reference
to Make or Take within the Public Customer and Broker Dealer portions
of the BOX Fee Schedule. Again, this proposal will not modify the fees
that are currently charged for the trading of options contracts on BOX,
nor will it change to whom the fees are charged.
2. Statutory Basis
    The Exchange believes that the proposal is consistent with the
requirements of Section 6(b) of the Act,\6\ in general, and Section
6(b)(5) of the Act,\7\ in particular, in that it clarifies existing
rule text and it is designed to promote just and equitable principles
of trade, to prevent fraudulent and manipulative acts, to remove
impediments to and perfect the mechanism of a free and open market and
a national market system and, in general, to protect investors and the
public interest.
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    \6\ 15 U.S.C. 78f(b).
    \7\ 15 U.S.C. 78f(b)(5).
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B. Self-Regulatory Organization's Statement on Burden on Competition

    The Exchange does not believe that the proposed rule change will
impose any burden on competition that is not necessary or appropriate
in furtherance of the purposes of the Act.

C. Self-Regulatory Organization's Statement on Comments on the Proposed
Rule Change Received From Members, Participants or Others

    The Exchange has neither solicited nor received comments on the
proposed rule change.

III. Date of Effectiveness of the Proposed Rule Change and Timing for
Commission Action

    The foregoing rule change has become effective pursuant to Section
19(b)(3)(A)(i) of the Act \8\ and subparagraph (f)(1) of Rule 19b-4
thereunder.\9\ The proposed rule change is a stated policy, practice or
interpretation with respect to the meaning, administration or
enforcement of an existing rule of the Exchange.
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    \8\ 15 U.S.C. 78s(b)(3)(A)(i).
    \9\ 17 CFR 240.19b-4(f)(1).
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    At any time within 60 days of the filing of such proposed rule
change, the Commission may summarily abrogate such rule change if it
appears to the Commission that such action is necessary or appropriate
in the public interest, for the protection of investors, or otherwise
in furtherance of the purposes of the Act.

Solicitation of Comments

    Interested persons are invited to submit written data, views, and
arguments concerning the foregoing, including whether the proposed rule
change is consistent with the Act. Comments may be submitted by any of
the following methods:

Electronic Comments

     Use the Commission's Internet comment form (http://
www.sec.gov/rules/sro.shtml); or
     Send an e-mail to<EMAIL_ADDRESS>Please include
File Number SR-BSE-2008-13 on the subject line.

Paper Comments

     Send paper comments in triplicate to Nancy M. Morris,
Secretary, Securities and Exchange Commission, 100 F Street, NE.,
Washington, DC 20549-1090.

All submissions should refer to File Number SR-BSE-2008-13. This file
number should be included on the subject line if e-mail is used. To
help the Commission process and review your comments more efficiently,
please use only one method. The Commission will post all comments on
the Commission's Internet Web site (http://www.sec.gov/rules/
sro.shtml). Copies of the submission, all subsequent amendments, all
written statements with respect to the proposed rule change that are
filed with the Commission, and all written communications relating to
the proposed rule change between the Commission and any person, other
than those that may be withheld from the public in accordance with the
provisions of 5 U.S.C. 552, will be available for inspection and
copying in the Commission's Public Reference Room, 100 F Street, NE.,
Washington, DC 20549, on official business days between the hours of 10
a.m. and 3 p.m. Copies of such filing also will be available for
inspection and copying at the principal office of BSE. All comments
received will be posted without change; the Commission does not edit
personal identifying information from submissions. You should submit
only information that you wish to make available publicly. All
submissions should refer to File Number SR-BSE-2008-13 and should be
submitted on or before April 4, 2008.

    For the Commission, by the Division of Trading and Markets,
pursuant to delegated authority.\10\
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    \10\ 17 CFR 200.30-3(a)(12).
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Florence E. Harmon,
Deputy Secretary.
 [FR Doc. E8-5098 Filed 3-13-08; 8:45 am]

BILLING CODE 8011-01-P


[Federal Register Volume 81, Number 28 (Thursday, February 11, 2016)]
[Notices]
[Pages 7335-7336]
[FR Doc No: 2016-02755]

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DEPARTMENT OF ENERGY

Federal Energy Regulatory Commission

Combined Notice of Filings #1

    Take notice that the Commission received the following electric
rate filings:

    Docket Numbers: ER15-1825-000.
    Applicants: California Independent System Operator Corporation.
    Description: Petition for Limited Waiver to Modify Effective Date
and Request for Shortened Comment Period of California Independent
System Operator Corporation.
    Filed Date: 2/4/16.
    Accession Numbers: 20160204-5236, 20160205-5049.
    Comments Due: 5 p.m. ET 2/11/16.

    Docket Numbers: ER16-898-000.
    Applicants: Northern States Power Company, a Minnesota corporation.
    Description: Tariff Cancellation: 20160204_Rate Schedule
601_Cancellation to be effective 4/30/2015.
    Filed Date: 2/4/16.
    Accession Number: 20160204-5212.
    Comments Due: 5 p.m. ET 2/25/16.

    Docket Numbers: ER16-899-000.
    Applicants: Orange and Rockland Utilities, Inc.
    Description: Notice of Cancellation of Power Supply Agreement, Rate
Schedule FERC No. 60, and Request for Waivers of Orange and Rockland
Utilities, Inc.
    Filed Date: 2/4/16.
    Accession Number: 20160204-5225.
    Comments Due: 5 p.m. ET 2/25/16.

    Docket Numbers: ER16-900-000.
    Applicants: Midcontinent Independent System Operator, Inc.
    Description: Section 205(d) Rate Filing: 2016-02-05_SA 2893 Black
Hawk MPFCA (J233, J274, J278, J279) to be effective 2/6/2016.
    Filed Date: 2/5/16.
    Accession Number: 20160205-5083.
    Comments Due: 5 p.m. ET 2/26/16.

    The filings are accessible in the Commission's eLibrary system by
clicking on the links or querying the docket number.
    Any person desiring to intervene or protest in any of the above
proceedings must file in accordance with Rules 211

and 214 of the Commission's Regulations (18 CFR 385.211 and 385.214) on
or before 5:00 p.m. Eastern time on the specified comment date.
Protests may be considered, but intervention is necessary to become a
party to the proceeding.
    eFiling is encouraged. More detailed information relating to filing
requirements, interventions, protests, service, and qualifying
facilities filings can be found at: http://www.ferc.gov/docs-filing/efiling/filing-req.pdf. For other information, call<PHONE_NUMBER>
(toll free). For TTY, call<PHONE_NUMBER>.

    Dated: February 5, 2016.
Nathaniel J. Davis, Sr.,
Deputy Secretary.
[FR Doc. 2016-02755 Filed 2-10-16; 8:45 am]
 BILLING CODE 6717-01-P


[Federal Register Volume 79, Number 138 (Friday, July 18, 2014)]
[Notices]
[Pages 42022-42023]
[FR Doc No: 2014-16931]

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DEPARTMENT OF HEALTH AND HUMAN SERVICES

Food and Drug Administration

[Docket No. FDA-2014-D-0903]

Draft Guidance for Industry: Providing Submissions in Electronic
Format--Postmarketing Safety Reports for Vaccines; Availability

AGENCY: Food and Drug Administration, HHS.

ACTION: Notice.

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SUMMARY: The Food and Drug Administration (FDA) is announcing the
availability of a draft document entitled ``Guidance for Industry:
Providing Submissions in Electronic Format--Postmarketing Safety
Reports for Vaccines'' dated July 2014. The draft guidance document
provides information and recommendations pertaining to the electronic
submission of postmarketing safety reports involving vaccine products
marketed for human use with approved biologics license applications
(BLAs), including individual case safety reports (ICSRs) and
attachments to ICSRs (ICSR attachments), into the Vaccine Adverse Event
Reporting System (VAERS). FDA recently published in the Federal
Register a final rule requiring that certain postmarketing safety
reports for human drug and biological products, including vaccines, be
submitted to FDA in an electronic format that the Agency can process,
review, and archive. The draft guidance, when finalized, is intended to
help applicants required to submit postmarketing safety reports comply
with the final rule. The draft guidance, when finalized, also will
supersede the document entitled ``Guidance for Industry: How to
Complete the Vaccine Adverse Event Report System Form (VAERS-1)'' dated
September 1998.

DATES: Although you can comment on any guidance at any time (see 21 CFR
10.115(g)(5)), to ensure that the Agency considers your comment on this
draft guidance before it begins work on the final version of the
guidance, submit either electronic or written comments on the draft
guidance by September 16, 2014.

ADDRESSES: Submit written requests for single copies of the draft
guidance to the Office of Communication, Outreach and Development,
Center for Biologics Evaluation and Research (CBER), Food and Drug
Administration, 10903 New Hampshire Ave., Bldg. 71, Rm. 3128, Silver
Spring, MD 20993-0002. Send one self-addressed adhesive label to assist
the office in processing your requests. The draft guidance may also be
obtained by mail by calling CBER at 1-800-835-4709 or<PHONE_NUMBER>. See
the SUPPLEMENTARY INFORMATION section for electronic access to the
draft guidance document.
    Submit electronic comments on the draft guidance to http://www.regulations.gov. Submit written comments to the Division of Dockets
Management (HFA-305), Food and Drug Administration, 5630 Fishers Lane,
Rm. 1061, Rockville, MD 20852.

FOR FURTHER INFORMATION CONTACT: John Reilly, Center for Biologics
Evaluation and Research, Food and Drug Administration, 10903 New
Hampshire Ave., Bldg. 71, Rm. 7301, Silver Spring, MD 20993-0002, 240-
402-7911.

SUPPLEMENTARY INFORMATION:

I. Background

    FDA is announcing the availability of a draft document entitled
``Guidance for Industry: Providing Submissions in Electronic Format--
Postmarketing Safety Reports for Vaccines'' dated July 2014. The draft
guidance provides information and recommendations pertaining to the
electronic submission of postmarketing safety reports involving vaccine
products, including ICSRs and ICSR attachments, into VAERS. The
guidance is applicable to vaccine products marketed for human use with
approved BLAs for which CBER has regulatory responsibility. This
guidance does not apply to any other biologic product.
    In the Federal Register of June 10, 2014 (79 FR 33072), FDA
published a final rule requiring that certain postmarketing safety
reports for human drug and biological products, including vaccines, be
submitted to FDA in an electronic format that the Agency can process,
review, and archive. The draft guidance, when finalized, is intended to
help applicants subject to postmarketing safety reporting requirements
comply with the final rule. Along with other information, the draft
guidance provides updated information about the following: (1) Options
for submitting ICSRs and ICSR attachments, as well as other
postmarketing safety reports to FDA in electronic format, (2) the
notification sent to submitters when FDA has received the electronic
postmarketing safety report, and (3) procedures for requesting
temporary waivers from the electronic submission requirement. The draft
guidance, when finalized, also will supersede the document entitled
``Guidance for Industry: How to Complete the Vaccine Adverse Event
Reporting System Form (VAERS-1)'' dated September 1998.
    The draft guidance is being issued consistent with FDA's good
guidance practices regulation (21 CFR 10.115). The draft guidance, when
finalized, will represent FDA's current thinking on this topic. It does
not create or confer any rights for or on any person and does not
operate to bind FDA or the public. An alternative approach may be used
if such approach satisfies the requirement of the applicable statutes
and regulations.

II. Paperwork Reduction Act of 1995

    The information collection resulting from this draft guidance is
covered by the information collection provisions of the June 10, 2014,
final rule entitled ``Postmarketing Safety Reports for Human Drug and
Biological Products; Electronic Submission Requirements.'' The
information collection provisions of the final rule have been submitted
to the Office of Management and Budget (OMB) for review, as required
under section 3507(d) of the Paperwork Reduction Act. Prior to the
effective date of the final rule, FDA will publish a notice in the
Federal Register announcing OMB's decision to approve, modify, or
disapprove the information collection provisions in the final rule. An
Agency may not conduct or sponsor, and a person is not required to
respond to, a collection of information unless it displays a currently
valid OMB control number.

III. Comments

    The draft guidance is being distributed for comment purposes only
and is not intended for implementation at this time. Interested persons
may submit either electronic comments regarding this document to http://www.regulations.gov or written comments to the Division of Dockets
Management (see ADDRESSES). It is only necessary to send one set of
comments. Identify comments with the docket number found in brackets in
the heading of this document. Received comments may be seen in the
Division of Dockets Management between 9 a.m. and 4 p.m., Monday
through Friday, and will be posted to the docket at http://www.regulations.gov.

IV. Electronic Access

    Persons with access to the Internet may obtain the draft guidance
at either http://www.fda.gov/BiologicsBloodVaccines/GuidanceComplianceRegulatoryInformation/Guidances/default.htm or http://www.regulations.gov.

    Dated: July 14, 2014.
Peter Lurie,
Associate Commissioner for Policy and Planning.
[FR Doc. 2014-16931 Filed 7-17-14; 8:45 am]
BILLING CODE 4164-01-P


2. Coliform (total), number per     MPN, 5 tube, 3 dilution,    p. 114 3.............  9221 B-2014..........  B-0025-85 4.........
 100 mL.                             or.                        p. 108 3.............  9222 B-2015 27.......
                                    MF 2, single step or two
                                     step.
                                    MF 2 with enrichment......  p. 111 3.............  9222 B- 2015 27......
3. E. coli, number per 100 mL.....  MPN 5 7 13, multiple tube,  .....................  9221 B.3-2014/9221 F-
                                     or.                                                2014 10 12 32.
                                    Multiple tube/multiple      .....................  9223 B-2016 11.......  991.15 9............  Colilert[supreg] 11
                                     well, or.                                                                                15, Colilert-
                                                                                18[supreg].11 14 15
                                    MF 2 5 6 7, two step, or..  1103.1 18............  9222 B-2015/9222 I-    D5392-93 8..........
                                                                                2015 17, 9213 D-2007.
                                    Single step...............  1603 19, 1604 20.....  .....................  ....................  m-ColiBlue24[supreg]
                                                                                16, KwikCountTM EC.
                                                                                28 29
4. Fecal streptococci, number per   MPN, 5 tube, 3 dilution,    p. 139 3.............  9230 B-2013..........  B-0055-85 4.........
 100 mL.                             or.                        p. 136 3.............  9230 C-2013 30.......
                                    MF 2, or..................
                                    Plate count...............  p. 143 3.............
5. Enterococci, number per 100 mL.  MPN 5 7, multiple tube/     .....................  9230 D-2013..........  D6503-99 8..........  Enterolert[supreg].1
                                     multiple well, or.         1106.1 22............  9230 C-2013 30.......  D5259-92 8..........   1 21
                                    MF 2 5 6 7 two step, or...
                                    Single step, or...........  1600 23..............  9230 C-2013 30.......
                                    Plate count...............  p. 143 3.............
--------------------------------------------------------------------------------------------------------------------------------------------------------
                                                                        Protozoa
--------------------------------------------------------------------------------------------------------------------------------------------------------
6. Cryptosporidium................  Filtration/IMS/FA.........  1622 24, 1623 25,
                                                                 1623.1 25 31.
7. Giardia........................  Filtration/IMS/FA.........  1623 25, 1623.1 25 31
--------------------------------------------------------------------------------------------------------------------------------------------------------
Table 1H notes:
\1\ The method must be specified when results are reported.
\2\ A 0.45-[micro]m membrane filter (MF) or other pore size certified by the manufacturer to fully retain organisms to be cultivated and to be free of
  extractables which could interfere with their growth.
\3\ Microbiological Methods for Monitoring the Environment, Water and Wastes. EPA/600/8-78/017. 1978. US EPA.
\4\ U.S. Geological Survey Techniques of Water-Resource Investigations, Book 5, Laboratory Analysis, Chapter A4, Methods for Collection and Analysis of
  Aquatic Biological and Microbiological Samples. 1989. USGS.
\5\ Tests must be conducted to provide organism enumeration (density). Select the appropriate configuration of tubes/filtrations and dilutions/volumes
  to account for the quality, character, consistency, and anticipated organism density of the water sample.
\6\ When the MF method has not been used previously to test waters with high turbidity, large numbers of noncoliform bacteria, or samples that may
  contain organisms stressed by chlorine, a parallel test should be conducted with a multiple-tube technique to demonstrate applicability and
  comparability of results.
\7\ To assess the comparability of results obtained with individual methods, it is suggested that side-by-side tests be conducted across seasons of the
  year with the water samples routinely tested in accordance with the most current Standard Methods for the Examination of Water and Wastewater or EPA
  alternate test procedure (ATP) guidelines.
\8\ Annual Book of ASTM Standards--Water and Environmental Technology. Section 11.02. 2000, 1999, 1996. ASTM International.
\9\ Official Methods of Analysis of AOAC International, 16th Edition, Volume I, Chapter 17. 1995. AOAC International.

\10\ The multiple-tube fermentation test is used in 9221B.3-2014. Lactose broth may be used in lieu of lauryl tryptose broth (LTB), if at least 25
  parallel tests are conducted between this broth and LTB using the water samples normally tested, and this comparison demonstrates that the false-
  positive rate and false-negative rate for total coliform using lactose broth is less than 10 percent. No requirement exists to run the completed phase
  on 10 percent of all total coliform-positive tubes on a seasonal basis.
\11\ These tests are collectively known as defined enzyme substrate tests.
\12\ After prior enrichment in a presumptive medium for total coliform using 9221B.3-2014, all presumptive tubes or bottles showing any amount of gas,
  growth or acidity within 48 h  3 h of incubation shall be submitted to 9221F-2014. Commercially available EC-MUG media or EC media
  supplemented in the laboratory with 50 [micro]g/mL of MUG may be used.
\13\ Samples shall be enumerated by the multiple-tube or multiple-well procedure. Using multiple-tube procedures, employ an appropriate tube and
  dilution configuration of the sample as needed and report the Most Probable Number (MPN). Samples tested with Colilert[supreg] may be enumerated with
  the multiple-well procedures, Quanti-Tray[supreg] or Quanti-Tray[supreg]/2000, and the MPN calculated from the table provided by the manufacturer.
\14\ Colilert-18[supreg] is an optimized formulation of the Colilert[supreg] for the determination of total coliforms and E. coli that provides results
  within 18 h of incubation at 35 [deg]C, rather than the 24 h required for the Colilert[supreg] test, and is recommended for marine water samples.
\15\ Descriptions of the Colilert[supreg], Colilert-18[supreg], Quanti-Tray[supreg], and Quanti-Tray[supreg]/2000 may be obtained from IDEXX
  Laboratories Inc.
\16\ A description of the mColiBlue24[supreg] test may be obtained from Hach Company.
\17\ Subject coliform positive samples determined by 9222B-2015 or other membrane filter procedure to 9222I-2015 using NA-MUG media.
\18\ Method 1103.1: Escherichia coli (E. coli) in Water by Membrane Filtration Using membrane-Thermotolerant Escherichia coli Agar (mTEC), EPA-821-R-10-
  002. March 2010. US EPA.
\19\ Method 1603: Escherichia coli (E. coli) in Water by Membrane Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar (Modified
  mTEC), EPA-821-R-14-010. September 2014. US EPA.
\20\ Method 1604: Total Coliforms and Escherichia coli (E. coli) in Water by Membrane Filtration by Using a Simultaneous Detection Technique (MI
  Medium), EPA 821-R-02-024. September 2002. US EPA.
\21\ A description of the Enterolert[supreg] test may be obtained from IDEXX Laboratories Inc.
\22\ Method 1106.1: Enterococci in Water by Membrane Filtration Using membrane-Enterococcus-Esculin Iron Agar (mE-EIA), EPA-821-R-09-015. December 2009.
  US EPA.
\23\ Method 1600: Enterococci in Water by Membrane Filtration Using membrane-Enterococcus Indoxyl-[beta]-D-Glucoside Agar (mEI), EPA-821-R-14-011.
  September 2014. US EPA.
\24\ Method 1622 uses a filtration, concentration, immunomagnetic separation of oocysts from captured material, immunofluorescence assay to determine
  concentrations, and confirmation through vital dye staining and differential interference contrast microscopy for the detection of Cryptosporidium.
  Method 1622: Cryptosporidium in Water by Filtration/IMS/FA, EPA-821-R-05-001. December 2005. US EPA.
\25\ Methods 1623 and 1623.1 use a filtration, concentration, immunomagnetic separation of oocysts and cysts from captured material, immunofluorescence
  assay to determine concentrations, and confirmation through vital dye staining and differential interference contrast microscopy for the simultaneous
  detection of Cryptosporidium and Giardia oocysts and cysts. Method 1623: Cryptosporidium and Giardia in Water by Filtration/IMS/FA. EPA-821-R-05-002.
  December 2005. US EPA. Method 1623.1: Cryptosporidium and Giardia in Water by Filtration/IMS/FA. EPA 816-R-12-001. January 2012. US EPA.
\26\ On a monthly basis, at least ten blue colonies from positive samples must be verified using Lauryl Tryptose Broth and EC broth, followed by count
  adjustment based on these results; and representative non-blue colonies should be verified using Lauryl Tryptose Broth. Where possible, verifications
  should be done from randomized sample sources.
\27\ On a monthly basis, at least ten sheen colonies from positive samples must be verified using Lauryl Tryptose Broth and brilliant green lactose bile
  broth, followed by count adjustment based on these results; and representative non-sheen colonies should be verified using Lauryl Tryptose Broth.
  Where possible, verifications should be done from randomized sample sources.
\28\ A description of KwikCountTM EC may be obtained from Micrology Laboratories LLC.
\29\ Approved for the analyses of E. coli in freshwater only.
\30\ Verification of colonies by incubation of BHI agar at 10  0.5 [deg]C for 48  3 h is optional. As per the Errata to the 23rd
  Edition of Standard Methods for the Examination of Water and Wastewater ``Growth on a BHI agar plate incubated at 10  0.5 [deg]C for 48
   3 h is further verification that the colony belongs to the genus Enterococcus.''
\31\ Method 1623.1 includes updated acceptance criteria for IPR, OPR, and MS/MSD and clarifications and revisions based on the use of Method 1623 for
  years and technical support questions.
\32\ 9221 F.2-2014 allows for simultaneous detection of E. coli and thermotolerant fecal coliforms by adding inverted vials to EC-MUG; the inverted
  vials collect gas produced by thermotolerant fecal coliforms.

    (b) Certain material is incorporated by reference into this section
with the approval of the Director of the Federal Register under 5
U.S.C. 552(a) and 1 CFR part 51. All approved material may be inspected
at EPA's Water Docket, EPA West, 1301 Constitution Avenue NW, Room
3334, Washington, DC 20004, (Telephone:<PHONE_NUMBER>). It is also
available for inspection at National Archives and Records
Administration (NARA). For information on the availability of this
material at NARA, call<PHONE_NUMBER>, or go to: http://www.archives.gov/federal-register/cfr/ibr-locations.html.
* * * * *
    (8) Office of Water, U.S. Environmental Protection Agency,
Washington, DC (U.S. EPA). Available at https://www.epa.gov/cwa-methods.
* * * * *
    (ix) Method 1623.1: Cryptosporidium and Giardia in Water by
Filtration/IMS/FA. EPA 816-R-12-001. January 2012. U.S. EPA, Table IH,
Note 25.
    (x) Method 1627, Kinetic Test Method for the Prediction of Mine
Drainage Quality. December 2011. EPA-821-R-09-002. Table IB, Note 69.
    (xi) Method 1664, n-Hexane Extractable Material (HEM; Oil and
Grease) and Silica Gel Treated n-Hexane Extractable Material (SGT-HEM;
Nonpolar Material) by Extraction and Gravimetry. Revision A, February
1999. EPA-821-R-98-002. Table IB, Notes 38 and 42.
    (xii) Method 1664, n-Hexane Extractable Material (HEM; Oil and
Grease) and Silica Gel Treated n-Hexane Extractable Material (SGT-HEM;
Nonpolar Material) by Extraction and Gravimetry, Revision B, February
2010. EPA-821-R-10-001. Table IB, Notes 38 and 42.
    (xiii) Method 1669, Sampling Ambient Water for Trace Metals at EPA
Water Quality Criteria Levels. July 1996. Table IB, Note 43.
    (xiv) Method 1680: Fecal Coliforms in Sewage Sludge (Biosolids) by
Multiple-Tube Fermentation using Lauryl Tryptose Broth (LTB) and EC
Medium. September 2014. EPA-821-R-14-009.Table IA, Note 15.
    (xv) Method 1681: Fecal Coliforms in Sewage Sludge (Biosolids) by
Multiple-Tube Fermentation using A-1 Medium. July 2006. EPA 821-R-06-
013. Table IA, Note 20.
    (xvi) Method 1682: Salmonella in Sewage Sludge (Biosolids) by
Modified Semisolid Rappaport-Vassiliadis (MSRV) Medium. September 2014.
EPA 821-R-14-012. Table IA, Note 23.
* * * * *
    (10) * * *
    (xiv) 2540, solids. 2015. Table IB.
* * * * *
    (xxxix) 4500-CN-, Cyanide. 2016. Table IB.
* * * * *
    (xliv) 4500-NO3-, Nitrogen (Nitrate). 2016. Table IB.
* * * * *
    (xlvi) 4500-O, Oxygen (Dissolved). 2016. Table IB.
* * * * *
    (lii) 5210, Biochemical Oxygen Demand (BOD). 2016. Table IB.
* * * * *
    (liv) 5310, Total Organic Carbon (TOC). 2014. Table IB.
* * * * *
    (lxvii) 9221 Multiple-Tube Fermentation Technique for Members of
the Coliform Group. 2014. Table IA, Notes 12 and 14; Table IH, Notes 10
and 12.
    (lxviii) 9222, Membrane Filter Technique for Members of the
Coliform Group. 2015. Table IA; Table IH, Note 17.

    (lxix) 9223 Enzyme Substrate Coliform Test. 2016. Table IA; Table
IH.
    (lxx) 9230 Fecal Enterococcus/Streptococcus Groups. 2013. Table IA;
Table IH.
* * * * *
    (15) * * *
    (v) ASTM D511-14, Standard Test Methods for Calcium and Magnesium
in Water. November 2014. Table IB.
    (vi) ASTM D512-12, Standard Test Methods for Chloride Ion in Water.
July 2012. Table IB.
* * * * *
    (viii) ASTM D516-16, Standard Test Method for Sulfate Ion in Water,
June 2016. Table IB.
    (ix) ASTM D858-17, Standard Test Methods for Manganese in Water.
June 2017. Table IB.
    (x) ASTM D859-16, Standard Test Method for Silica in Water. June
2016. Table IB.
    (xi) ASTM D888-12, Standard Test Methods for Dissolved Oxygen in
Water. March 2012. Table IB.
    (xii) ASTM D1067-16, Standard Test Methods for Acidity or
Alkalinity of Water. June 2016. Table IB.
    (xiii) ASTM D1068-15, Standard Test Methods for Iron in Water.
October 2015. Table IB.
* * * * *
    (xv) ASTM D1126-17, Standard Test Method for Hardness in Water.
December 2017. Table IB.
    (xvi) ASTM D1179-16, Standard Test Methods for Fluoride Ion in
Water. June 2016. Table IB.
    (xvii) ASTM D1246-16, Standard Test Method for Bromide Ion in
Water. June 2016. Table IB.
    (xviii) ASTM D1252-06 (Reapproved 2012), Standard Test Methods for
Chemical Oxygen Demand (Dichromate Oxygen Demand) of Water. June 2012.
Table IB.
    (xix) ASTM D1253-14, Standard Test Method for Residual Chlorine in
Water. February 2014. Table IB.
* * * * *
    (xxi) ASTM D1426-15, Standard Test Methods for Ammonia Nitrogen in
Water. April 2015. Table IB.
    (xxii) ASTM D1687-17, Standard Test Methods for Chromium in Water.
July 2017. Table IB.
    (xxiii) ASTM D1688-17, Standard Test Methods for Copper in Water.
July 2017. Table IB.
    (xxiv) ASTM D1691-17, Standard Test Methods for Zinc in Water. June
2017. Table IB.
    (xxv) ASTM D1783-01 (Reapproved 2012), Standard Test Methods for
Phenolic Compounds in Water. August 2012. Table IB.
    (xxvi) ASTM D1886-14, Standard Test Methods for Nickel in Water.
November 2014. Table IB.
* * * * *
    (xxxi) ASTM D2036-09 (Reapproved 2015), Standard Test Methods for
Cyanides in Water. July 2015. Table IB.
* * * * *
    (xxxiv) ASTM D2972-15, Standard Tests Method for Arsenic in Water.
March 2015. Table IB.
    (xxxv) ASTM D3223-17, Standard Test Method for Total Mercury in
Water. June 2017. Table IB.
* * * * *
    (xxxvii) ASTM D3373-17, Standard Test Method for Vanadium in Water.
June 2017. Table IB.
* * * * *
    (xxxix) ASTM D3557-17, Standard Test Method for Cadmium in Water.
June 2017. Table IB.
    (xl) ASTM D3558-15, Standard Test Method for Cobalt in Water. March
2015. Table IB.
    (xli) ASTM D3559-15, Standard Test Methods for Lead in Water.
October 2015. Table IB.
    (xlii) ASTM D3590-17, Standard Test Methods for Total Kjeldahl
Nitrogen in Water. June 2017. Table IB.
    (xliii) ASTM D3645-15, Standard Test Methods for Beryllium in
Water. March 2015. Table IB.
* * * * *
    (xlv) ASTM D3859-15, Standard Test Methods for Selenium in Water.
April 2015. Table IB.
    (xlvi) ASTM D3867-16, Standard Test Method for Nitrite-Nitrate in
Water. June 2016. Table IB.
    (xlvii) ASTM D4190-15, Standard Test Method for Elements in Water
by Direct- Current Plasma Atomic Emission Spectroscopy. March 2015.
Table IB.
    (xlviii) ASTM D4282-15, Standard Test Method for Determination of
Free Cyanide in Water and Wastewater by Microdiffusion. July 2015.
Table IB.
    (xlix) ASTM D4327-17, Standard Test Method for Anions in Water by
Suppressed Ion Chromatography. December 2017. Table IB.
    (l) ASTM D4382-18, Standard Test Method for Barium in Water, Atomic
Absorption Spectrophotometry, Graphite Furnace. May 2018. Table IB.
* * * * *
    (lii) ASTM D4658-15, Standard Test Method for Sulfide Ion in Water.
April 2015. Table IB.
* * * * *
    (liv) ASTM D4839-03 (Reapproved 2017), Standard Test Method for
Total Carbon and Organic Carbon in Water by Ultraviolet, or Persulfate
Oxidation, or Both, and Infrared Detection. December 2017. Table IB.
    (lv) ASTM D5257-17, Standard Test Method for Dissolved Hexavalent
Chromium in Water by Ion Chromatography. December 2017. Table IB.
* * * * *
    (lviii) ASTM D5673-16, Standard Test Method for Elements in Water
by Inductively Coupled Plasma--Mass Spectrometry. February 2016. Table
IB.
* * * * *
    (lxi) ASTM. D6508-15, Standard Test Method for Determination of
Dissolved Inorganic Anions in Aqueous Matrices Using Capillary Ion
Electrophoresis and Chromate Electrolyte. October 2015. Table IB, Note
54.
    (lxii) ASTM. D6888-16, Standard Test Method for Available Cyanide
with Ligand Displacement and Flow Injection Analysis (FIA) Utilizing
Gas Diffusion Separation and Amperometric Detection. June 2016. Table
IB, Note 59.
    (lxiii) ASTM. D6919-17, Standard Test Method for Determination of
Dissolved Alkali and Alkaline Earth Cations and Ammonium in Water and
Wastewater by Ion Chromatography. June 2017. Table IB.
    (lxiv) ASTM. D7065-17, Standard Test Method for Determination of
Nonylphenol, Bisphenol A, p-tert-Octylphenol, Nonylphenol
Monoethoxylate and Nonylphenol Diethoxylate in Environmental Waters by
Gas Chromatography Mass Spectrometry. January 2018. Table IC.
    (lxv) ASTM. D7237-15a, Standard Test Method for Free Cyanide with
Flow Injection Analysis (FIA) Utilizing Gas Diffusion Separation and
Amperometric Detection. June 2015. Table IB.
    (lxvi) ASTM. D7284-13 (Reapproved 2017), Standard Test Method for
Total Cyanide in Water by Micro Distillation followed by Flow Injection
Analysis with Gas Diffusion Separation and Amperometric Detection. July
2017. Table IB.
* * * * *
    (lxviii) ASTM. D7511-12 (Reapproved 2017), Standard Test Method for
Total Cyanide by Segmented Flow Injection Analysis, In-Line Ultraviolet
Digestion and Amperometric Detection. July 2017. Table IB.
    (lxix) ASTM. D7573-09 (Reapproved 2017), Standard Test Method for
Total Carbon and Organic Carbon in Water by High Temperature Catalytic
Combustion and Infrared Detection, February 2017. Table IB.
    (lxx) ASTM D7781-14 Standard Test Method for Nitrate-Nitrite in
Water by Nitrate Reductase, May 2014. Table IB.
* * * * *

    (19) FIAlab Instruments, Inc., 2151 N. Northlake Way, Seattle, WA
98103. Telephone:<PHONE_NUMBER>.
    (i) Method 100, Determination of Inorganic Ammonia by Continuous
Flow Gas Diffusion and Fluorescence Detector Analysis, April 4, 2018.
Table IB, Note 82.
    (ii) [Reserved]
* * * * *
    (26) MACHEREY-NAGEL GmbH and Co., 2850 Emrick Blvd. Bethlehem, PA
18020. Telephone:<PHONE_NUMBER>.
    (i) Method 036/038 NANOCOLOR[supreg] COD LR/HR, Spectrophotometric
Measurement of Chemical Oxygen Demand in Water and Wastewater, Revision
1.5, May 2018. Table IB, Note 83.
    (ii) [Reserved]
    (27) Micrology Laboratories, LLC, 1303 Eisenhower Drive, Goshen, IN
46526. Telephone:<PHONE_NUMBER>.
    (i) KwikCountTM EC Medium E. coli enzyme substrate test,
Rapid Detection of E. coli in Beach Water By KwikCountTM EC
Membrane Filtration. 2014. Table IH, Notes 28 and 29.
    (ii) [Reserved]
* * * * *
    (38) * * *
    (ii) Determination of Heat Purgeable and Ambient Purgeable Volatile
Organic Compounds in Water by Gas Chromatography/Mass Spectrometry.
Chapter 12 of Section B, Methods of the National Water Quality
Laboratory, of Book 5, Laboratory Analysis. 2016.
    (iii) Methods for Determination of Inorganic Substances in Water
and Fluvial Sediments, editors, Techniques of Water-Resources
Investigations of the U.S. Geological Survey, Book 5, Chapter A1. 1979.
Table IB, Note 8.
    (iv) Methods for Determination of Inorganic Substances in Water and
Fluvial Sediments, Techniques of Water-Resources Investigations of the
U.S. Geological Survey, Book 5, Chapter A1. 1989. Table IB, Notes 2 and
79.
    (v) Methods for the Determination of Organic Substances in Water
and Fluvial Sediments. Techniques of Water-Resources Investigations of
the U.S. Geological Survey, Book 5, Chapter A3. 1987. Table IB, Note
24; Table ID, Note 4.
    (vi) OFR 76-177, Selected Methods of the U.S. Geological Survey of
Analysis of Wastewaters. 1976. Table IE, Note 2.
    (vii) OFR 91-519, Methods of Analysis by the U.S. Geological Survey
National Water Quality Laboratory--Determination of Organonitrogen
Herbicides in Water by Solid-Phase Extraction and Capillary-Column Gas
Chromatography/Mass Spectrometry With Selected-Ion Monitoring. 1992.
Table ID, Note 14.
    (viii) OFR 92-146, Methods of Analysis by the U.S. Geological
Survey National Water Quality Laboratory--Determination of Total
Phosphorus by a Kjeldahl Digestion Method and an Automated Colorimetric
Finish That Includes Dialysis. 1992. Table IB, Note 48.
    (ix) OFR 93-125, Methods of Analysis by the U.S. Geological Survey
National Water Quality Laboratory--Determination of Inorganic and
Organic Constituents in Water and Fluvial Sediments. 1993. Table IB,
Note 51 and 80; Table IC, Note 9.
    (x) OFR 93-449, Methods of Analysis by the U.S. Geological Survey
National Water Quality Laboratory--Determination of Chromium in Water
by Graphite Furnace Atomic Absorption Spectrophotometry. 1993. Table
IB, Note 46.
    (xi) OFR 94-37, Methods of Analysis by the U.S. Geological Survey
National Water Quality Laboratory--Determination of Triazine and Other
Nitrogen-containing Compounds by Gas Chromatography with Nitrogen
Phosphorus Detectors. 1994. Table ID, Note 9.
    (xii) OFR 95-181, Methods of Analysis by the U.S. Geological Survey
National Water Quality Laboratory--Determination of Pesticides in Water
by C-18 Solid-Phase Extraction and Capillary-Column Gas Chromatography/
Mass Spectrometry With Selected-Ion Monitoring. 1995. Table ID, Note
11.
    (xiii) OFR 97-198, Methods of Analysis by the U.S. Geological
Survey National Water Quality Laboratory--Determination of Molybdenum
in Water by Graphite Furnace Atomic Absorption Spectrophotometry. 1997.
Table IB, Note 47.
    (xiv) OFR 97-829, Methods of Analysis by the U.S. Geological Survey
National Water Quality Laboratory-- Determination of 86 Volatile
Organic Compounds in Water by Gas Chromatography/Mass Spectrometry,
Including Detections Less Than Reporting Limits. 1999. Table IC, Note
13.
    (xv) OFR 98-165, Methods of Analysis by the U.S. Geological Survey
National Water Quality Laboratory--Determination of Elements in Whole-
Water Digests Using Inductively Coupled Plasma-Optical Emission
Spectrometry and Inductively Coupled Plasma-Mass Spectrometry. 1998.
Table IB, Notes 50 and 81.
    (xvi) OFR 98-639, Methods of Analysis by the U.S. Geological Survey
National Water Quality Laboratory--Determination of Arsenic and
Selenium in Water and Sediment by Graphite Furnace--Atomic Absorption
Spectrometry. 1999. Table IB, Note 49.
    (xvii) OFR 00-170, Methods of Analysis by the U.S. Geological
Survey National Water Quality Laboratory--Determination of Ammonium
Plus Organic Nitrogen by a Kjeldahl Digestion Method and an Automated
Photometric Finish that Includes Digest Cleanup by Gas Diffusion. 2000.
Table IB, Note 45.
    (xviii) Techniques and Methods Book 5-B1, Determination of Elements
in Natural-Water, Biota, Sediment and Soil Samples Using Collision/
Reaction Cell Inductively Coupled Plasma-Mass Spectrometry. Chapter 1,
Section B, Methods of the National Water Quality Laboratory, Book 5,
Laboratory Analysis. 2006. Table IB, Note 70.
    (xix) U.S. Geological Survey Techniques of Water-Resources
Investigations, Book 5, Laboratory Analysis, Chapter A4, Methods for
Collection and Analysis of Aquatic Biological and Microbiological
Samples. 1989. Table IA, Note 4; Table IH, Note 4.
    (xx) Water-Resources Investigation Report 01-4098, Methods of
Analysis by the U.S. Geological Survey National Water Quality
Laboratory--Determination of Moderate-Use Pesticides and Selected
Degradates in Water by C-18 Solid-Phase Extraction and Gas
Chromatography/Mass Spectrometry. 2001. Table ID, Note 13.
    (xxi) Water-Resources Investigations Report 01-4132, Methods of
Analysis by the U.S. Geological Survey National Water Quality
Laboratory--Determination of Organic Plus Inorganic Mercury in Filtered
and Unfiltered Natural Water With Cold Vapor-Atomic Fluorescence
Spectrometry. 2001. Table IB, Note 71.
    (xxii) Water-Resources Investigation Report 01-4134, Methods of
Analysis by the U.S. Geological Survey National Water Quality
Laboratory--Determination of Pesticides in Water by Graphitized Carbon-
Based Solid-Phase Extraction and High-Performance Liquid
Chromatography/Mass Spectrometry. 2001. Table ID, Note 12.
    (xxiii) Water Temperature--Influential Factors, Field Measurement
and Data Presentation, Techniques of Water-Resources Investigations of
the U.S. Geological Survey, Book 1, Chapter D1. 1975. Table IB, Note
32.
* * * * *
    (e) * * *

                    Table II--Required Containers, Preservation Techniques, and Holding Times
----------------------------------------------------------------------------------------------------------------
                                                                                Maximum holding time 4
        Parameter number/name                Container 1            Preservation 2 3
----------------------------------------------------------------------------------------------------------------
                                            Table IA--Bacterial Tests
----------------------------------------------------------------------------------------------------------------
1-4. Coliform, total, fecal, and E.    PA, G..................  Cool, <10 [deg]C,        8 hours.22 23
 coli.                                                           0.008% Na2S2O3 5.
5. Fecal streptococci................  PA, G..................  Cool, <10 [deg]C,        8 hours.22
                                                                 0.008% Na2S2O3 5.
6. Enterococci.......................  PA, G..................  Cool, <10 [deg]C,        8 hours.22
                                                                 0.008% Na2S2O3 5.
7. Salmonella........................  PA, G..................  Cool, <10 [deg]C,        8 hours.22
                                                                 0.008% Na2S2O3 5.
----------------------------------------------------------------------------------------------------------------
                                        Table IA--Aquatic Toxicity Tests
----------------------------------------------------------------------------------------------------------------
8-11. Toxicity, acute and chronic....  P, FP, G...............  Cool, <=6 [deg]C 16....  36 hours.
----------------------------------------------------------------------------------------------------------------
                                            Table IB--Inorganic Tests
----------------------------------------------------------------------------------------------------------------
1. Acidity...........................  P, FP, G...............  Cool, <=6 [deg]C 18....  14 days.
2. Alkalinity........................  P, FP, G...............  Cool, <=6 [deg]C 18....  14 days.
4. Ammonia...........................  P, FP, G...............  Cool, <=6 [deg]C 18,     28 days.
                                                                 H2SO4 to pH <2.
9. Biochemical oxygen demand.........  P, FP, G...............  Cool, <=6 [deg]C 18....  48 hours.
10. Boron............................  P, FP, or Quartz.......  HNO3 to pH <2..........  6 months.
11. Bromide..........................  P, FP, G...............  None required..........  28 days.
14. Biochemical oxygen demand,         P, FP G................  Cool, <=6 [deg]C 18....  48 hours.
 carbonaceous.
15. Chemical oxygen demand...........  P, FP, G...............  Cool, <=6 [deg]C 18,     28 days.
                                                                 H2SO4 to pH <2.
16. Chloride.........................  P, FP, G...............  None required..........  28 days.
17. Chlorine, total residual.........  P, G...................  None required..........  Analyze within 15
                                                                                minutes.
21. Color............................  P, FP, G...............  Cool, <=6 [deg]C 18....  48 hours.
23-24. Cyanide, total or available     P, FP, G...............  Cool, <=6 [deg]C 18,     14 days.
 (or CATC) and free.                                             NaOH to pH >10 5 6,
                                                                 reducing agent if
                                                                 oxidizer present.
25. Fluoride.........................  P......................  None required..........  28 days.
27. Hardness.........................  P, FP, G...............  HNO3 or H2SO4 to pH <2.  6 months.
28. Hydrogen ion (pH)................  P, FP, G...............  None required..........  Analyze within 15
                                                                                minutes.
31, 43. Kjeldahl and organic N.......  P, FP, G...............  Cool, <=6 [deg]C 18,     28 days.
                                                                 H2SO4 to pH <2.
----------------------------------------------------------------------------------------------------------------
                                               Table IB--Metals 7
----------------------------------------------------------------------------------------------------------------
18. Chromium VI......................  P, FP, G...............  Cool, <=6 [deg]C 18, pH  28 days.
                                                                 = 9.3-9.7 20.
35. Mercury (CVAA)...................  P, FP, G...............  HNO3 to pH <2..........  28 days.
35. Mercury (CVAFS)..................  FP, G; and FP-lined cap  5 mL/L 12N HCl or 5 mL/  90 days.17
                                        17.                      L BrCl 17.
3, 5-8, 12, 13, 19, 20, 22, 26, 29,    P, FP, G...............  HNO3 to pH <2, or at     6 months.
 30, 32-34, 36, 37, 45, 47, 51, 52,                              least 24 hours prior
 58-60, 62, 63, 70-72, 74, 75.                                   to analysis 19.
 Metals, except boron, chromium VI,
 and mercury.
38. Nitrate..........................  P, FP, G...............  Cool, <=6 [deg]C 18....  48 hours.
39. Nitrate-nitrite..................  P, FP, G...............  Cool, <=6 [deg]C 18,     28 days.
                                                                 H2SO4 to pH <2.
40. Nitrite..........................  P, FP, G...............  Cool, <=6 [deg]C 18....  48 hours.
41. Oil and grease...................  G......................  Cool to <=6 [deg]C 18,   28 days.
                                                                 HCl or H2SO4 to pH <2.
42. Organic Carbon...................  P, FP, G...............  Cool to <=6 [deg]C 18,   28 days.
                                                                 HCl, H2SO4, or H3PO4
                                                                 to pH <2.
44. Orthophosphate...................  P, FP, G...............  Cool, to <=6 [deg]C 18   Filter within 15
                                                                 24.                      minutes; Analyze
                                                                                within 48 hours.
46. Oxygen, Dissolved Probe..........  G, Bottle and top......  None required..........  Analyze within 15
                                                                                minutes.
47. Winkler..........................  G, Bottle and top......  Fix on site and store    8 hours.
                                                                 in dark.
48. Phenols..........................  G......................  Cool, <=6 [deg]C 18,     28 days.
                                                                 H2SO4 to pH <2.
49. Phosphorus (elemental)...........  G......................  Cool, <=6 [deg]C 18....  48 hours.
50. Phosphorus, total................  P, FP, G...............  Cool, <=6 [deg]C 18,     28 days.
                                                                 H2SO4 to pH <2.
53. Residue, total...................  P, FP, G...............  Cool, <=6 [deg]C 18....  7 days.
54. Residue, Filterable (TDS)........  P, FP, G...............  Cool, <=6 [deg]C 18....  7 days.
55. Residue, Nonfilterable (TSS).....  P, FP, G...............  Cool, <=6 [deg]C 18....  7 days.
56. Residue, Settleable..............  P, FP, G...............  Cool, <=6 [deg]C 18....  48 hours.
57. Residue, Volatile................  P, FP, G...............  Cool, <=6 [deg]C 18....  7 days.
61. Silica...........................  P or Quartz............  Cool, <=6 [deg]C 18....  28 days.
64. Specific conductance.............  P, FP, G...............  Cool, <=6 [deg]C 18....  28 days.
65. Sulfate..........................  P, FP, G...............  Cool, <=6 [deg]C 18....  28 days.
66. Sulfide..........................  P, FP, G...............  Cool, <=6 [deg]C 18,     7 days.
                                                                 add zinc acetate plus
                                                                 sodium hydroxide to pH
                                                                 >9.
67. Sulfite..........................  P, FP, G...............  None required..........  Analyze within 15
                                                                                minutes.
68. Surfactants......................  P, FP, G...............  Cool, <=6 [deg]C 18....  48 hours.
69. Temperature......................  P, FP, G...............  None required..........  Analyze within 15
                                                                                minutes.
73. Turbidity........................  P, FP, G...............  Cool, <=6 [deg]C 18....  48 hours.
----------------------------------------------------------------------------------------------------------------

                                            Table IC--Organic Tests 8
----------------------------------------------------------------------------------------------------------------
13, 18-20, 22, 24, 25, 27, 28, 34-37,  G, FP-lined septum.....  Cool, <=6 [deg]C 18,     14 days.9
 39-43, 45-47, 56, 76, 104, 105, 108-                            0.008% Na2S2O3 5, HCl
 111, 113. Purgeable Halocarbons.                                to pH 2 9.
26. 2-Chloroethylvinyl ether.........  G, FP-lined septum.....  Cool, <=6 [deg]C 18,     14 days.
                                                                 0.008% Na2S2O3 5.
6, 57, 106. Purgeable aromatic         G, FP-lined septum.....  Cool, <=6 [deg]C 18,     14 days.9
 hydrocarbons.                                                   0.008% Na2S2O3 5, HCl
                                                                 to pH 2 9.
3, 4. Acrolein and acrylonitrile.....  G, FP-lined septum.....  Cool, <=6 [deg]C 18,     14 days.10
                                                                 0.008% Na2S2O3, pH to
                                                                 4-5 10.
23, 30, 44, 49, 53, 77, 80, 81, 98,    G, FP-lined cap........  Cool, <=6 [deg]C 18,     7 days until
 100, 112. Phenols 11.                                           0.008% Na2S2O3.          extraction, 40 days
                                                                                after extraction.
7, 38. Benzidines 11 12..............  G, FP-lined cap........  Cool, <=6 [deg]C 18,     7 days until
                                                                 0.008% Na2S2O3 5.        extraction.13
14, 17, 48, 50-52. Phthalate esters    G, FP-lined cap........  Cool, <=6 [deg]C 18....  7 days until
 11.                                                                                extraction, 40 days
                                                                                after extraction.
82-84. Nitrosamines 11 14............  G, FP-lined cap........  Cool, <=6 [deg]C 18,     7 days until
                                                                 store in dark, 0.008%    extraction, 40 days
                                                                 Na2S2O3 5.               after extraction.
88-94. PCBs 11.......................  G, FP-lined cap........  Cool, <=6 [deg]C 18....  1 year until
                                                                                extraction, 1 year
                                                                                after extraction.
54, 55, 75, 79. Nitroaromatics and     G, FP-lined cap........  Cool, <=6 [deg]C 18,     7 days until
 isophorone 11.                                                  store in dark, 0.008%    extraction, 40 days
                                                                 Na2S2O3 5.               after extraction.
1, 2, 5, 8-12, 32, 33, 58, 59, 74,     G, FP-lined cap........  Cool, <=6 [deg]C 18,     7 days until
 78, 99, 101. Polynuclear aromatic                               store in dark, 0.008%    extraction, 40 days
 hydrocarbons 11.                                                Na2S2O3 5.               after extraction.
15, 16, 21, 31, 87. Haloethers 11....  G, FP-lined cap........  Cool, <=6 [deg]C 18,     7 days until
                                                                 0.008% Na2S2O3 5.        extraction, 40 days
                                                                                after extraction.
29, 35-37, 63-65, 73, 107.             G, FP-lined cap........  Cool, <=6 [deg]C 18....  7 days until
 Chlorinated hydrocarbons 11.                                                             extraction, 40 days
                                                                                after extraction.
60-62, 66-72, 85, 86, 95-97, 102,      G......................  See footnote 11........  See footnote 11.
 103. CDDs/CDFs 11.
----------------------------------------------------------------------------------------------------------------
    Aqueous Samples: Field and Lab     G......................  Cool, <=6 [deg]C 18,     1 year.
     Preservation.                                               0.008% Na2S2O3 5, pH
                                                                 <9.
    Solids and Mixed-Phase Samples:    G......................  Cool, <=6 [deg]C 18....  7 days.
     Field Preservation.
    Tissue Samples: Field              G......................  Cool, <=6 [deg]C 18....  24 hours.
     Preservation.
    Solids, Mixed-Phase, and Tissue    G......................  Freeze, <=-10 [deg]C...  1 year.
     Samples: Lab Preservation.
114-118. Alkylated phenols...........  G......................  Cool, <6 [deg]C, H2SO4   28 days until
                                                                 to pH <2.                extraction, 40 days
                                                                                after extraction.
119. Adsorbable Organic Halides (AOX)  G......................  Cool, <6 [deg]C, 0.008%  Hold at least 3 days,
                                                                 Na2S2O3, HNO3 to pH <2.  but not more than 6
                                                                                months.
120. Chlorinated Phenolics...........  G, FP-lined cap........  Cool, <6 [deg]C, 0.008%  30 days until
                                                                 Na2S2O3, H2SO4 to pH     acetylation, 30 days
                                                                 <2.                      after acetylation.
----------------------------------------------------------------------------------------------------------------
                                           Table ID--Pesticides Tests
----------------------------------------------------------------------------------------------------------------
1-70. Pesticides 11..................  G, FP-lined cap........  Cool, <=6 [deg]C 18, pH  7 days until
                                                                 5-9 15.                  extraction, 40 days
                                                                                after extraction.
----------------------------------------------------------------------------------------------------------------
                                          Table IE--Radiological Tests
----------------------------------------------------------------------------------------------------------------
1-5. Alpha, beta, and radium.........  P, FP, G...............  HNO3 to pH <2..........  6 months.
----------------------------------------------------------------------------------------------------------------
                                            Table IH--Bacterial Tests
----------------------------------------------------------------------------------------------------------------
1, 2. Coliform, total, fecal.........  PA, G..................  Cool, <10 [deg]C,        8 hours.22
                                                                 0.008% Na2S2O3 5.
3.E. coli............................  PA, G..................  Cool, <10 [deg]C,        8 hours.22
                                                                 0.008% Na2S2O3 5.
4. Fecal streptococci................  PA, G..................  Cool, <10 [deg]C,        8 hours.22
                                                                 0.008% Na2S2O3 5.
5. Enterococci.......................  PA, G..................  Cool, <10 [deg]C,        8 hours.22
                                                                 0.008% Na2S2O3 5.
----------------------------------------------------------------------------------------------------------------
                                            Table IH--Protozoan Tests
----------------------------------------------------------------------------------------------------------------
6. Cryptosporidium...................  LDPE; field filtration.  1-10 [deg]C............  96 hours.21
7. Giardia...........................  LDPE; field filtration.  1-10 [deg]C............  96 hours.21
----------------------------------------------------------------------------------------------------------------
1 ''P'' is for polyethylene; ``FP'' is fluoropolymer (polytetrafluoroethylene [PTFE]; Teflon[supreg]), or other
  fluoropolymer, unless stated otherwise in this Table II; ``G'' is glass; ``PA'' is any plastic that is made of
  a sterilizable material (polypropylene or other autoclavable plastic); ``LDPE'' is low density polyethylene.

2 Except where noted in this Table II and the method for the parameter, preserve each grab sample within 15
  minutes of collection. For a composite sample collected with an automated sample (e.g., using a 24-hour
  composite sample; see 40 CFR 122.21(g)(7)(i) or 40 CFR part 403, appendix E), refrigerate the sample at <=6
  [deg]C during collection unless specified otherwise in this Table II or in the method(s). For a composite
  sample to be split into separate aliquots for preservation and/or analysis, maintain the sample at <=6 [deg]C,
  unless specified otherwise in this Table II or in the method(s), until collection, splitting, and preservation
  is completed. Add the preservative to the sample container prior to sample collection when the preservative
  will not compromise the integrity of a grab sample, a composite sample, or aliquot split from a composite
  sample within 15 minutes of collection. If a composite measurement is required but a composite sample would
  compromise sample integrity, individual grab samples must be collected at prescribed time intervals (e.g., 4
  samples over the course of a day, at 6-hour intervals). Grab samples must be analyzed separately and the
  concentrations averaged. Alternatively, grab samples may be collected in the field and composited in the
  laboratory if the compositing procedure produces results equivalent to results produced by arithmetic
  averaging of results of analysis of individual grab samples. For examples of laboratory compositing
  procedures, see EPA Method 1664 Rev. A (oil and grease) and the procedures at 40 CFR 141.24(f)(14)(iv) and (v)
  (volatile organics).
3 When any sample is to be shipped by common carrier or sent via the U.S. Postal Service, it must comply with
  the Department of Transportation Hazardous Materials Regulations (49 CFR part 172). The person offering such
  material for transportation is responsible for ensuring such compliance. For the preservation requirement of
  Table II, the Office of Hazardous Materials, Materials Transportation Bureau, Department of Transportation has
  determined that the Hazardous Materials Regulations do not apply to the following materials: Hydrochloric acid
  (HCl) in water solutions at concentrations of 0.04% by weight or less (pH about 1.96 or greater; Nitric acid
  (HNO3) in water solutions at concentrations of 0.15% by weight or less (pH about 1.62 or greater); Sulfuric
  acid (H2SO4) in water solutions at concentrations of 0.35% by weight or less (pH about 1.15 or greater); and
  Sodium hydroxide (NaOH) in water solutions at concentrations of 0.080% by weight or less (pH about 12.30 or
  less).
4 Samples should be analyzed as soon as possible after collection. The times listed are the maximum times that
  samples may be held before the start of analysis and still be considered valid. Samples may be held for longer
  periods only if the permittee or monitoring laboratory have data on file to show that, for the specific types
  of samples under study, the analytes are stable for the longer time, and has received a variance from the
  Regional ATP Coordinator under Sec.   136.3(e). For a grab sample, the holding time begins at the time of
  collection. For a composite sample collected with an automated sampler (e.g., using a 24-hour composite
  sampler; see 40 CFR 122.21(g)(7)(i) or 40 CFR part 403, appendix E), the holding time begins at the time of
  the end of collection of the composite sample. For a set of grab samples composited in the field or
  laboratory, the holding time begins at the time of collection of the last grab sample in the set. Some samples
  may not be stable for the maximum time period given in the table. A permittee or monitoring laboratory is
  obligated to hold the sample for a shorter time if it knows that a shorter time is necessary to maintain
  sample stability. See Sec.   136.3(e) for details. The date and time of collection of an individual grab
  sample is the date and time at which the sample is collected. For a set of grab samples to be composited, and
  that are all collected on the same calendar date, the date of collection is the date on which the samples are
  collected. For a set of grab samples to be composited, and that are collected across two calendar dates, the
  date of collection is the dates of the two days; e.g., November 14-15. For a composite sample collected
  automatically on a given date, the date of collection is the date on which the sample is collected. For a
  composite sample collected automatically, and that is collected across two calendar dates, the date of
  collection is the dates of the two days; e.g., November 14-15. For static-renewal toxicity tests, each grab or
  composite sample may also be used to prepare test solutions for renewal at 24 h, 48 h, and/or 72 h after first
  use, if stored at 0-6 [deg]C, with minimum head space.
5 ASTM D7365-09a specifies treatment options for samples containing oxidants (e.g., chlorine) for cyanide
  analyses. Also, Section 9060A of Standard Methods for the Examination of Water and Wastewater (23rd edition)
  addresses dechlorination procedures for microbiological analyses.
6 Sampling, preservation and mitigating interferences in water samples for analysis of cyanide are described in
  ASTM D7365-09a (15). There may be interferences that are not mitigated by the analytical test methods or D7365-
  09a (15). Any technique for removal or suppression of interference may be employed, provided the laboratory
  demonstrates that it more accurately measures cyanide through quality control measures described in the
  analytical test method. Any removal or suppression technique not described in D7365-09a (15) or the analytical
  test method must be documented along with supporting data.
7 For dissolved metals, filter grab samples within 15 minutes of collection and before adding preservatives. For
  a composite sample collected with an automated sampler (e.g., using a 24-hour composite sampler; see 40 CFR
  122.21(g)(7)(i) or 40 CFR part 403, appendix E), filter the sample within 15 minutes after completion of
  collection and before adding preservatives. If it is known or suspected that dissolved sample integrity will
  be compromised during collection of a composite sample collected automatically over time (e.g., by interchange
  of a metal between dissolved and suspended forms), collect and filter grab samples to be composited (footnote
  2) in place of a composite sample collected automatically.
8 Guidance applies to samples to be analyzed by GC, LC, or GC/MS for specific compounds.
9 If the sample is not adjusted to pH 2, then the sample must be analyzed within seven days of sampling.
10 The pH adjustment is not required if acrolein will not be measured. Samples for acrolein receiving no pH
  adjustment must be analyzed within 3 days of sampling.
11 When the extractable analytes of concern fall within a single chemical category, the specified preservative
  and maximum holding times should be observed for optimum safeguard of sample integrity (i.e., use all
  necessary preservatives and hold for the shortest time listed). When the analytes of concern fall within two
  or more chemical categories, the sample may be preserved by cooling to <=6 [deg]C, reducing residual chlorine
  with 0.008% sodium thiosulfate, storing in the dark, and adjusting the pH to 6-9; samples preserved in this
  manner may be held for seven days before extraction and for forty days after extraction. Exceptions to this
  optional preservation and holding time procedure are noted in footnote 5 (regarding the requirement for
  thiosulfate reduction), and footnotes 12, 13 (regarding the analysis of benzidine).
12 If 1,2-diphenylhydrazine is likely to be present, adjust the pH of the sample to 4.0  0.2 to
  prevent rearrangement to benzidine.
13 Extracts may be stored up to 30 days at <0 [deg]C.
14 For the analysis of diphenylnitrosamine, add 0.008% Na2S2O3 and adjust pH to 7-10 with NaOH within 24 hours
  of sampling.
15 The pH adjustment may be performed upon receipt at the laboratory and may be omitted if the samples are
  extracted within 72 hours of collection. For the analysis of aldrin, add 0.008% Na2S2O3.
16 Place sufficient ice with the samples in the shipping container to ensure that ice is still present when the
  samples arrive at the laboratory. However, even if ice is present when the samples arrive, immediately measure
  the temperature of the samples and confirm that the preservation temperature maximum has not been exceeded. In
  the isolated cases where it can be documented that this holding temperature cannot be met, the permittee can
  be given the option of on-site testing or can request a variance. The request for a variance should include
  supportive data which show that the toxicity of the effluent samples is not reduced because of the increased
  holding temperature. Aqueous samples must not be frozen. Hand-delivered samples used on the day of collection
  do not need to be cooled to 0 to 6 [deg]C prior to test initiation.
17 Samples collected for the determination of trace level mercury (<100 ng/L) using EPA Method 1631 must be
  collected in tightly-capped fluoropolymer or glass bottles and preserved with BrCl or HCl solution within 48
  hours of sample collection. The time to preservation may be extended to 28 days if a sample is oxidized in the
  sample bottle. A sample collected for dissolved trace level mercury should be filtered in the laboratory
  within 24 hours of the time of collection. However, if circumstances preclude overnight shipment, the sample
  should be filtered in a designated clean area in the field in accordance with procedures given in Method 1669.
  If sample integrity will not be maintained by shipment to and filtration in the laboratory, the sample must be
  filtered in a designated clean area in the field within the time period necessary to maintain sample
  integrity. A sample that has been collected for determination of total or dissolved trace level mercury must
  be analyzed within 90 days of sample collection.
18 Aqueous samples must be preserved at <=6 [deg]C, and should not be frozen unless data demonstrating that
  sample freezing does not adversely impact sample integrity is maintained on file and accepted as valid by the
  regulatory authority. Also, for purposes of NPDES monitoring, the specification of ``<= [deg]C'' is used in
  place of the ``4 [deg]C'' and ``<4 [deg]C'' sample temperature requirements listed in some methods. It is not
  necessary to measure the sample temperature to three significant figures (1/100th of 1 degree); rather, three
  significant figures are specified so that rounding down to 6 [deg]C may not be used to meet the <=6 [deg]C
  requirement. The preservation temperature does not apply to samples that are analyzed immediately (less than
  15 minutes).

19 An aqueous sample may be collected and shipped without acid preservation. However, acid must be added at
  least 24 hours before analysis to dissolve any metals that adsorb to the container walls. If the sample must
  be analyzed within 24 hours of collection, add the acid immediately (see footnote 2). Soil and sediment
  samples do not need to be preserved with acid. The allowances in this footnote supersede the preservation and
  holding time requirements in the approved metals methods.
20 To achieve the 28-day holding time, use the ammonium sulfate buffer solution specified in EPA Method 218.6.
  The allowance in this footnote supersedes preservation and holding time requirements in the approved
  hexavalent chromium methods, unless this supersession would compromise the measurement, in which case
  requirements in the method must be followed.
21 Holding time is calculated from time of sample collection to elution for samples shipped to the laboratory in
  bulk and calculated from the time of sample filtration to elution for samples filtered in the field.
22 Sample analysis should begin as soon as possible after receipt; sample incubation must be started no later
  than 8 hours from time of collection.
23 For fecal coliform samples for sewage sludge (biosolids) only, the holding time is extended to 24 hours for
  the following sample types using either EPA Method 1680 (LTB-EC) or 1681 (A-1): Class A composted, Class B
  aerobically digested, and Class B anaerobically digested.
24 The immediate filtration requirement in orthophosphate measurement is to assess the dissolved or bio-
  available form of orthophosphorus (i.e., that which passes through a 0.45-micron filter), hence the
  requirement to filter the sample immediately upon collection (i.e., within 15 minutes of collection).

0
3. Amend Sec.  136.6 by adding paragraph (b)(4)(xxiii) to read as
follows:

Sec.  136.6   Method modifications and analytical requirements.

* * * * *
    (b) * * *
    (4) * * *
    (xxiii) When analyzing metals by inductively coupled plasma-atomic
emission spectroscopy, inductively coupled plasma-mass spectrometry,
and stabilized temperature graphite furnace atomic absorption, closed-
vessel microwave digestion of wastewater samples is allowed as
alternative heating source for Method 200.2--``Sample Preparation
Procedure for Spectrochemical Determination of Total Recoverable
Elements'' for the following elements: Aluminum, antimony, arsenic,
barium, beryllium, boron, cadmium, calcium, chromium, cobalt, copper,
iron, lead, magnesium, manganese, molybdenum, nickel, potassium,
selenium, silver, sodium, thallium, tin, titanium, vanadium, zinc,
provided the performance specifications in the relevant determinative
method are met. (Note that this list does not include Mercury.) Each
laboratory determining total recoverable metals is required to operate
a formal quality control (QC) program. The minimum requirements include
initial demonstration of capability, method detection limit (MDL),
analysis of reagent blanks, fortified blanks, matrix spike samples, and
blind proficiency testing samples, as continuing quality control checks
on performance. The laboratory is required to maintain performance
records on file that define the quality of the data generated.
* * * * *
[FR Doc. 2021-09596 Filed 5-18-21; 8:45 am]
BILLING CODE 6560-50-P