Seven tests were conducted with green algae; 96 h EC50 values ( based on growth rate, cell density, cell counts, and dry weights) ranged from 1.2 to > 666 mg/ L ( the Er50 cell density value of 1,000 mg/ L is excluded from this discussion). The lower value indicates high to moderate toxicity, based on the acute criteria. The lower value would also be indicative of moderate toxicity, based on the chronic moderate criterion (. 0.1< 10 mg/ L). A 14 d EC50 value of 43 mg/ L, based on cell counts, for green algae was also calculated in one study. This is indicative of low chronic toxicity, based on the chronic criterion ( 10 mg/ L). Green algae appeared to be the most sensitive test species in the 44% APFO test sample, daphnids were the next most sensitive, and fathead minnows were the least sensitive. 83 Table 9. Summary of Acute Ecological Toxicity Data for APFO ( grouped by test substance) Test Organism Duration Value ( mg/ L)* Reference Test Sample: APFO ammonium salt 96 h LC50 70 3M Company, 1974a 96 h LC50 766 3M Company, 1980a 96 h LC50 301 3M Company, 1987c Fathead minnow ( Pimephales promelas)

96 h LC50 740 Ward et al.,, 1995 96 h LC50 > 420 3M Company, 1978 Bluegill sunfish ( Lepomis machrochirus) 96 h LC50 569 3M Company, 1978 48 h EC50 126 3M Environmental Laboratory, 1982 48 h EC50 > 1000 3M Environmental Laboratory, 1982 48 h EC50 221 3M Company, 1987b Water flea ( Daphnia magna)

48 h EC50 720 Ward et al., 1995 96 h EC50 310 Ward et al., 1995 Green algae ( Selenastrum capricornutum) 96 h EC50 1000 Ward et al., 1995 30 min EC50 870 3M Company, 1987a Bacteria ( Photobacterium phosphoreum) 30 min EC50 730 3M Environmental Laboratory, 1996a 7 min NOEC 1000 3M Company, 1980b Activated sludge 30 min EC50 > 1000 3M Company, 1987d Test Sample: APFO 96 h LC50 440 3M Company, 1974b Fathead minnow ( Pimephales promelas) 96 h LC50 843 3M Company, 1985 Test Sample: APFO ammonium salt in 50% water 96 h LC50 > 500 EnviroSystems, Inc., 1990a Fathead minnow ( Pimephales promelas)

96 h NOEC 500 EnviroSystems, Inc., 1990a Water flea ( Daphnia magna) 48 h EC50 292 EnviroSystems, Inc., 1990b Bacteria ( Photobacterium phosphoreum) 30 min EC50 > 500 3M Environmental Laboratory, 1990a Test Sample: APFO ammonium salt in 50% water, continued Activated sludge 30 min EC50 > 500 3M Environmental Laboratory, 1990b 84 Test Sample: APFO ammonium salt in 80% water Fathead minnow ( Pimephales promelas) 96 h LC50 494 Ward et al., 1996a Water flea ( Daphnia magna) 48 h EC50 240 Ward et al., 1996c 96 h EC50 396 Ward et al., 1996b Green algae ( Selenastrum capricornutum) 96 h EC50 > 666 Ward et al., 1996b 30 min EC50 630 3M Environmental Laboratory, 1996b Bacteria ( Photobacterium phosphoreum) 30 min EC50 390 3M Environmental Laboratory, 1996c Activated sludge 30 min EC50 > 664 3M Environmental Laboratory, 1996d Test Sample: APFO in 50% isopropanol Fathead minnow ( Pimephales promelas) 96h LC50 140 T. R. Wilbury Laboratories, Inc., 1996a Water flea ( Daphnia magna) 48 h EC50 360 T. R. Wilbury Laboratories, Inc., 1996b Green algae ( Selenastrum capricornutum) 96 h EC50 90 T. R. Wilbury Laboratories, Inc., 1995 Test Sample: APFO ( 44%) in 27.9% water and 27.2% isopropanol Fathead minnow ( Pimephales promelas) 96 h EC50 391 T. R. Wilbury Laboratories, Inc., 1995 Fathead minnow ( Pimephales promelas) 96 h EC50 422 T. R. Wilbury Laboratories, Inc., 1995 Test Sample: APFO ( 44%) in 27.9% water and 27.2% isopropanol Water flea ( Daphnia magna) 48 h EC50 41 Ward et al., 1995 Water flea ( Daphnia magna) 48 h EC50 39 Ward et al., 1995 Green algae ( Selenastrum capricornutum) 96 h EC50 2.1 Ward et al., 1995 Green algae ( Selenastrum capricornutum) 96 h EC50 3.6 Ward et al., 1995 Green algae ( Selenastrum capricornutum) 96 h EC50 1.2 Ward et al., 1995 * Values were adjusted to represent 100% active ingredient. AThese values may be inconsistent due to different diets tested. 85 Table 10. Summary of Ecological Toxicity Data for APFO ( grouped by species) Test Organism Duration Value ( mg/ L) Reference 96 h LC50 70B 3M Company, 1974a 96 h LC50 766B 3M Company, 1980a 96 h LC50 301B 3M Company, 1987c 96 h LC50 440C 3M Company, 1974b 96 h LC50 843C 3M Company, 1985 96 h LC50 > 500D EnviroSystems, Inc., 1990a 96 h NOEC 500D EnviroSystems, Inc., 1990a 96 h LC50 494 Ward et al., 1996a 96h LC50 140F T. R. Wilbury Laboratories, Inc., 1996a 30 day NOAEL > 100B EG& G Bionomics Aquatic Toxicology Laboratory, 1978 96 h EC50 391G T. R. Wilbury Laboratories, Inc., 1995 Fathead minnow ( Pimephales promelas)

96 h EC50 422G T. R. Wilbury Laboratories, Inc., 1995 96 h LC50 > 420B 3M Company, 1978 Bluegill sunfish ( Lepomis machrochirus) 96 h LC50 569B 3M Company, 1978 48 h EC50 126AB 3M Environmental Laboratory, 1982 48 h EC50 > 1000AB 3M Environmental Laboratory, 1982 48 h EC50 221B 3M Company, 1987b 48 h EC50 292D EnviroSystems, Inc., 1990b 48 h EC50 240 Ward et al., 1996c 48 h EC50 360F T. R. Wilbury Laboratories, Inc., 1996b 21 day IC50 43B 3M Company, 1984 21 day NOEC 22B 3M Company, 1984 21 day NOEC 22B 3M Company, 1984 48 h EC50 41G Ward et al., 1995 Water flea ( Daphnia magna)

48 h EC50 39G Ward et al., 1995 86 Table 10. Summary of Ecological Toxicity Data for APFO ( grouped by species) Test Organism Duration Value ( mg/ L) Reference 96 h EC50 396 Ward et al., 1996b 96 h EC50 > 666E Ward et al., 1996b 96 h EC50 90F T. R. Wilbury Laboratories, Inc., 1995 14 day EC50 43B Elnabarawy, 1981 96 h EC50 2.1G Ward et al., 1995 96 h EC50 3.6G Ward et al., 1995 Green algae ( Selenastrum capricornutum)

96 h EC50 1.2G Ward et al., 1995 30 min EC50 870B 3M Company, 1987a 30 min EC50 730B 3M Environmental Laboratory, 1996a 30 min EC50 > 500D 3M Environmental Laboratory, 1990a 30 min EC50 630 3M Environmental Laboratory, 1996b Bacteria ( Photobacterium phosphoreum)

30 min EC50 390 3M Environmental Laboratory, 1996c 7 min NOEC 1000B 3M Company, 1980b 30 min EC50 > 1000B 3M Company, 1987d 30 min EC50 > 500D 3M Environmental Laboratory, 1990b Activated sludge 30 min EC50 > 664E 3M Environmental Laboratory, 1996d * Values were adjusted to represent 100% active ingredient. AThese values may be inconsistent due to different diets tested. BTested substance was APFO ammonium salt. CTested substance was APFO DTested substance was APFO ammonium salt in 50% water. ETested substance was APFO ammonium salt in 80% water. FTested substance was APFO in 50% isopropanol. GTest Sample: APFO ( 44%) in 27.9% water and 27.2% isopropanol 87 5.0 References 3M Company. 1976a. Primary Eye Irritation Study Rabbits.

3M Company. 1976b. Acute Oral Toxicity in Rats T 1585.

3M Company. 1977. Ready Biodegradation of FC 143 ( BOD/ COD/ TOC). Environmental Laboratory. St. Paul, MN.

3M Company International Research and Development Corporation. 1978b. Fluorad Fluorochemical FC 143 Acute Oral Toxicity ( LD50) Study in Rats. Study No. 137 091,

3M Company. 1979. Technical Report Summary  Final Comprehensive Report: FC 143. ( USEPA AR 226 528)

3M Company. 1980c. Ready Biodegradation of FC 143( BOD/ COD). Lab Request No. 5625S. Environmental Laboratory. St. Paul, MN.

3M Company. 1981. Water, Acetone and Toluene Solubility Estimates. Environmental Laboratory. St. Paul, MN.

3M Company. 1984. Chronic toxicity to freshwater invertebrates.

3M Company. 1985a. 96 hour acute static toxicity to fathead minnow   FX 1001. Environmental Laboratory, St. Paul, MN. Lab Request Number C1006. February 2.

3M Company. 1985b. Ready Biodegradation of FX 1001 ( BOD/ COD). Lab Request No. C1006. Environmental Laboratory. St. Paul, MN. February 14.

3M Company. 1987. Activated Sludge Respiration Inhibition Test. Environmental Laboratory; Lab Request Number E1282. St. Paul, MN.

3M Company. 1999a. " The Science of Organic Fluorochemistry" and " Perfluorooctane sulfonate: Current summary of human sera, health, and toxicology data". February 5, 1999. ( 8EHQ 0299 373).

3M Company. 1999b. 8EHQ 0699 373. Supplement. May 26, 1999.

3M Company. 2000a. Voluntary Use and Exposure Information Profile for Perfluorooctanesulfonic Acid and Various Salt Forms. 3M Company submission to USEPA, dated April 27, 2000.

3M Company. 2000b. Voluntary Use and Exposure Information Profile for Perfluorooctanoic Acid and Salts. 3M Company submission to USEPA, dated June 8, 2000. 88 3M Company, 2000c. About 3M Worldwide: 3M Phasing Out Some of Its Specialty Materials. http:// www. 3M. com/ about3m/ worldwide/ release. html.

3M Company. 2001a. Environmental Monitoring  Multi City Study, 3M Environmental Laboratory, June 25. In U. S. EPA Administrative Record AR226 1030A.

3M Company, 2002. Submission dated September 17, 2002 to USEPA, AR 226.

3M Environmental Laboratory. 1990a. Microbics Microtox ® Toxicity Test. St. Paul, Minnesota. Lab request number G2882.

3M Environmental Laboratory. 1990b. Activated Sludge Respiration Inhibition. St. Paul, Minnesota. Lab Request number G2882.

3M Environmental Laboratory. 1993. Impinger Studies of Volatility of FC 95 and FC 143. St. Paul, MN. 3M Laboratories. 3M Lab Request Number L3306.

3M Environmental Laboratory. 1996a. Microbics Microtox ® Toxicity Test of FC 143. Lab Request number P1626. St. Paul, Minnesota.

3M Environmental Laboratory. 1996b. Microbics Microtox ® Toxicity Test of FC 118. Lab Request number P1626. St. Paul, Minnesota.

3M Environmental Laboratory. 1996c. Microbics Microtox ® Toxicity Test of FC 1015 X. Lab Request number P1626. St. Paul, Minnesota.

3M Environmental Laboratory. 2001a. Hydrolysis Reactions of Perfluorooctanoic Acid ( PFOA). Lab Request Number E00 1851. March 30.

3M Environmental Laboratory. 2001b. Characterization Study of PFOA ( lot # 332), Primary Standard   Test Control Reference # TCR 99030 030. Phase: Solubility Determination. 3M Laboratories, St. Paul, MN.

3M Environmental Laboratory 2001c. 26 Week Capsule Toxicity Study with Ammonium Perfluorooctanoate ( APFO) in Cynomolgus Monkeys Amended Analytical Laboratory Report Title Determination of the Presence and Concentration of Perfluorooctanoate Fluorochemical in Liver, Serum, Urine and Feces Samples.. Performing Laboratories, Liver, Serum and Urine Analyses, 3M Environmental Laboratories, St. Paul, MN, Feces Analyses Centre Analytical Laboratories, Inc. State College, PA. Analytical Phase Completion Date, June 11, 2001, 408 pp.

Abdellatif, A. G. Preat, V., Taper, H. S. and Roberfroid, M. 1991. The modulation of rat liver carcinogenesis by perfluorooctanoic acid, a peroxisome proliferator. Toxicol. Appl. Pharmacol. 111: 530 537. 89 Alexander, B. H. 2001a. Mortality study of workers employed at the 3M Cottage Grove facility. Final Report. Division of Environmental and Occupational Health, School of Public Health, University of Minnesota, April 26, 2001.

Alexander, B. H. 2001b. Mortality study of workers employed at the 3M Decatur facility. Final Report. Division of Environmental and Occupational Health, School of Public Health, University of Minnesota, April 26, 2001.

Behar, B.; Stein, G. Science 1966, Vol. 154, p. 1012.

Beach, S. 1995a. Inhibitory Effect of L 13492 to Microbics' MicrotoxTM Toxicity Analyzer System. 3M Company, Environmental Laboratory, St Paul, MN, Lab Request number N2169. July 26.

Beach, S. 1995b. Inhibitory Effect of L 13492 on Activated Sludge. 3M Company Environmental Laboratory, St. Paul, MN, Lab Request N2169, July 26.

Biegel, L. B., Liu, R.. C. M., Hurtt, M. E. and Cook, J. C. 1995. Effects of ammonium perfluorooctanate on Leydig cell function:, , and ex vivo studies. Toxicol. Appl. Pharmacol. 134: 18 25.

Biegel, L. B., Hurtt, M. E., Frame, S. R., O'Connor, J. C., and J. C. Cook. 2001. Mechanisms of extrahepatic tumor induction by peroxisome proliferators in male CD rats. Toxicological Sciences. 60: 44 55.

Bio/ dynamics Inc. 1979. An Acute Inhalation Study of T 2305 CoC in the Rat. 3M Company, St. Paul, MN. Project No. 78 7184.

Biosearch, Inc. 1976. Primary Eye Irritation Study   Rabbits. Philadelphia, PA. 3M Company. St. Paul, MN.

Boeri, R., Magazu, J., Ward, T. 1995a. Acute toxicity of L 13492 to the Daphnid, Daphnia Magna. T. R. Wilbury Laboratories, Inc., 3M Company Lab Request number N2332, July 13.

Boeri, R., Magazu, J., Ward, T. 1995b. Acute Toxicity of L 13492 to the Fathead Minnow, Pimephales promelas. T. R. Wilbury Laboratories, Inc., 3M Company Lab Request number N2332, July 13.

Boeri, R., Magazu, J., Ward, T. 1995c. Growth and reproduction Toxicity Test with L 13492 and the Freshwater Alga, Selenastrum capricornutum. T. R. Wilbury Laboratories, Inc., 3M Company Lab Request number N2332. August 3.

Boeri, R., Kowalski, P., Ward, T. 1995d. Acute Toxicity of N2803 2 to the Fathead Minnow, 90 Pimephales promelas. T. R. Wilbury Laboratories, Inc., 3M Company, Lab Request number N2803 2. November 16.

Boeri, R., Kowalski, P., Ward, T. 1995e. Acute Toxicity of N2803 4 to the Fathead Minnow, Pimephales promelas. T. R. Wilbury Laboratories, Inc., 3M Company Lab Request number N2803 4. November 21.

Boeri, R., Kowalski, P., Ward, T. 1995f. Growth and Reproduction Toxicity Test with N2803 2 and the Freshwater Algae, Selenastrum capicornutum. T. R. Wilbury Laboratories, Inc., 3M Company Lab Request number N2803 2. November 28.

Boeri, R., Kowalski, P., Ward, T. 1996a. Growth and Reproduction Toxicity Test with N2803 4 and the Freshwater Alga, Selenastrum capicornutum. T. R. Wilbury Laboratories, Inc., 3M Company Lab Request number N2803 4. March 7.

Boeri, R., Kowalski, P., Ward, T. 1996b. Acute Toxicity of N2803 4 to the Daphnid, Daphnia Magna. T. R. Wilbury Laboratories, Inc., 3M Company Lab Request number N2803 4. March 25.

Boeri, R., Kowalski, P., Ward, T. 1996c. Acute Toxicity of N2803 2 to the Daphnid, Daphnia magna. T. R. Wilbury Laboratories, Inc., 3M Company Lab Request Number N2803 2. March 25.

Boyd, S. 1993a. Review of Technical Report Summary: Adsorption of FC 95 and FC 143 in Soil. Michigan State University. May 19.

Boyd, S. A. 1993b. Review of Technical Notebook. Soil Thin Layer Chromatography. Number 48277, p30. Michigan State University.

Bultman, D. and Pike, M. of 3M " The Use of Fluorochemical Surfactants in Floor Polish", http:// home. hanmir. com/~ hahnw/ news/ 3m. html Burris, JM; Olsen, G; Simpson, C; Mandel, J. 2000 Determination of serum half lives of several fluorochemicals. Interim Report # 1, Corporate Occupational Medicine Department, 3M Company.

Burris, JM, Lundberg, JK, Olsen, GW, Simpson, C, Mandel, J. 2002. Determination of serum half lives of several fluorochemicals. Interim Report # 2. 3M Medical Department.

Butenhoff, J, Costa, G, Elcombe, C, Farrar, D, Hansen, K, Iwai, H, Jung, R, Kennedy, G, Lieder, P, Olsen, G, and Thomford, P. 2002. Toxicity of ammonium perfluorooctanoate in male cynomolgus monkeys after oral dosing for 6 months. Toxicol. Sci. 69: 244 257.

Cameron, R. G., Imaida, K., Tsuda, H. and Ito, N. 1982. Promotive effects of steroids and bile 91 acids on hepatocarcinogenesis initiated by diethylnitrosamine. Cancer Res. 42: 2426 2428.

Carlfors, J. et. al. Colloid Interface Sci. 103, 332  336.( 1985)

Chandra, M., Riley, M. G. I. and Johnson, D. E. 1992. Spontaneous neoplasms in aged spraguedawley rats. Arch. Toxiocol. 66: 496 502.

Chapin, R. E., Stevens, J. T., Hughes, C. L., Kelce, W. R., Hess, R. A. and Daston, G. P. 1996. Symposium overview: endocrine modulation of reproduction. Fund. Appl. Toxicol. 29: 1 17.

Chemguard, 2000. Chemguard Inc., Press Release Re: Chemguard Incorporated Announces Major Breakthrough in AFFF Fire Fighting Foam Technology, June 30, 2000.

Christopher, B. and Marias, A. J. 1977. 28 Day Oral Toxicity Study with FC 143 in Albino Mice, Final Report, Industrial Bio Test Laboratories, Inc. Study No. 8532 10655, 3M Reference No. T 1742CoC, Lot 269.

Clegg, E. D., Cook, J. C. , Chapin, R. E., Foster, P. M. D. and Daston, G. P. 1997. Leydig cell hyperplasia and adenoma formation: mechanisms and relevance to humans. Reproduct. Toxicol. 11: 107 121.

CLOGP( v4.71)   Calculation of hydrophobicity as Log P( o/ w). 2001. Daylight Chemical Information Systems, Inc. www. daylight. com Cook, J. C., Hurtt, M. E., Frame, S. R., and Biegel, L. B. 1994. Mechanisms of extrahepatic tumor induction by peroxisome proliferators in Crl: CD BR( CD) rats. Toxicologist, 14: 301, abstract # 1169.

Cutts, J. H. and Noble, R. L. 1964. Estrone induced mammary tumors in the rat. I. Induction and behavior of tumors. Cancer Res. 24: 1116 1123.

Daikin, 2001. Nobuhiko Tsuda, Daikin Industries Ltd., " Fluoropolymer Emulsion for High Performance Coatings" in Paint & Coating Industry Magazine, June 2001, p. 56 66.

DCP, 1998. Directory of World Chemical Producers: 1998 Edition. Chemical Information Services. Dallas, TX.

DuPont, 2000. Voluntary Use and Exposure Information Profile. DuPont submission to USEPA, dated June 23, 2000.

Dynax, 2000. Letter from Eduard Kleiner ( Dynax) to Charlie Auer ( USEPA), Re: Phase Out of 3M AFFF Agents, dated August 2, 2000.

Edwards, PJB et. al. LANGMUIR 13( 10), 2665 2669 ( 1997) 92 EG& G Bionomics Aquatic Toxicology Laboratory. 1978. The effects of continuous exposure to 78.03 on hatchability of eggs and growth and survival of fry of fathead minnow ( Pimephales promelas). Report # BW 78 6 175. Research report submitted to 3M Company, St. Paul, MN.

Ellis D. A., S. A. Mabury, J. W. Martin and D. C. G. Muir 2001. Thermolysis of fluoropolymers as a potential source of halogenated organic acids in the environment. Nature: 412, pp. 321 324.

Elnabarawy, M. T. 1981. 3M Technical Report Summary, Multi Phase Exposure/ Recovery Algal Assay Test Method. Report Number 006. Project Number 9970030000. October 16.

EnviroSystems, Inc. 1990a. Static Acute Toxicity of FX 1003 to the Fathead Minnow, Pimephales promelas. Hampton, NH. Study number was 9014 3.

EnviroSystems, Inc. 1990b. Static Acute Toxicity of FX 1003 to the Daphnid, Daphnia magna. Hampton, NH. EnviroSystems study number 9013 3.

ES& T, 2000. Cheryl Moody and Jennifer Field, " Perfluorinated Surfactants and the Environmental Implications of Their Use in Fire Fighting Foams" in Environmental Science & Technology, Vol. 34, Issue 18, p. 3864 3870.

FMG, 2001. Verbal comments by the Fluoropolymer Manufacturers Group of the Society of the Plastics Industry. FMG/ EPA meeting, March 7, 2001.

FMG Fluorochemical Manufacturers Group AR226 1094 2002 ( Fluoropolymer Manufacturers Group Presentation Slides).

Gabriel, Karl. Summary of: Primary Skin Irritation Study   Rabbits. Performed by: Biosearch. Submitted to 3M Company, 3M Center, St. Paul, MN.

Gabriel, Karl. Summary of: Primary Eye Irritation Study   Rabbits. Performed by: Biosearch. Submitted to 3M Company, 3M Center, St. Paul, MN.

Garry, V. F., and R. L. Nelson. 1981. An Assay of Cell Transformation and Cytotoxicity in C3H 10 ½ Clonal Cell Line for the Test Chemical T 2942 CoC. 3M Company, St. Paul, MN.

Geisy, J. P., Dr. 1995. 3M requested expert overview of " Bioaccumulative Properties of Ammonium Perfluorooctanoate: Static Fish Test". Geisy Ecotoxicology, Inc. March 20.

Giesy J. P. and K. Kannan, 2001a. Accumulation of perfluorooctanesulfonate and related fluorochemicals in fish tissues. Prepared for 3M, St. Paul MN. June 20. In U. S. EPA Administrative Record AR226 1030A Giesy J. P. and K. Kannan, 2001b. Perfluorooctanesulfonate and related fluorochemicals in fish 93 eating water birds. Prepared for 3M, St. Paul MN. June 20. In U. S. EPA Administrative Record AR226 1030A Giesy J. P. and K. Kannan, 2001c. Accumulation of perfluorooctanesulfonate and related fluorochemicals in mink and river otters. Prepared for 3M, St. Paul MN. June 20. In U. S. EPA Administrative Record AR226 1030A Giesy J. P. and K. Kannan, 2001d. Perfluorooctanesulfonate and related fluorochemicals in oyster, Crassostrea virginica, from the Gulf of Mexico and Chesapeake Bay. Prepared for 3M, St. Paul MN. June 20. In U. S. EPA Administrative Record AR226 1030A Giesy J. P. and J. L. Newsted, 2001e. Selected fluorochemicals in the Decatur, Alabama area. Prepared for 3M, St. Paul MN, Project 178401. June. In U. S. EPA Administrative Record AR226 1030A Gibson, S. J., and Johnson, J. D. 1979. Absorption of FC 143 14C In Rats After a Single Oral Dose. Riker Laboratories, Inc., Subsidiary of 3M, St. Paul, Minnesota.

Gibson, S. J., and Johnson, J. D. 1980. Extent and Route of Excretion and Tissue Distribution of Total Carbon 14 in Male and Female Rats After a Single IV Dose of FC 143 14C. Riker Laboratories, Inc., Subsidiary of 3M, St. Paul, Minnesota.

Gibson, S. J., and Johnson, J. D. 1983. Extent and Route of Excretion of Total Carbon 14 in Pregnant Rats After a Single Oral Dose of Ammonium 14 C Perfluorooctanoate. Riker Laboratories, Inc., Subsidiary of 3M, St. Paul, Minnesota.

Gillett, James. 1993. 3M requested expert review of " Bioaccumulation Studies". Cornell University. March 8.

Gilliland, F. 1992. Fluorochemicals and Human Health: Studies in an Occupational Cohort. Doctoral thesis, Division of Environmental and Occupational Health, University of Minnesota.

Gilliland, F. D. and Mandel, J. S. 1993. Mortality among employees of a perfluorooctanoic acid production plant. JOM. 35( 9): 950 954.

Gilliland, FD and Mandel, JS. 1996. Serum Perfluorooctanoic acid and hepatic enzymes, lipoproteins, and cholesterol: a study of occupationally exposed men. Am J Ind Med 29: 560 568.

Glaza, S. 1995. Acute dermal toxicity study of T 6342 in rabbits. Corning Hazelton, Inc. Madison, WI. Project ID: HWI 50800374. 3M Company. St. Paul, MN.

Glaza, S. M. 1997. Acute Oral Toxicity Study of T 6669 in Rats. Corning Hazleton Inc. CHW 61001760. January 10. Sponsored by 3M, St. Paul, Minnesota. 94 Goldenthal, E. I. 1978a. Ninety Day Subacute Rat Toxicity Study. Final Report Prepared for 3M, St Paul, Minnesota, by International Research and Development Corporation, St. Paul, Minnesota, November 6, 1978.

Goldenthal, E. I. 1978b. Ninety Day Subacute Rhesus Monkey Toxicity Study. Final Report Prepared for 3M, St Paul, Minnesota, by International Research and Development Corporation, St. Paul, Minnesota, November 10, 1978.

Gortner, E. G. 1981. Oral Teratology Study of T 2998CoC in Rats. Safety Evaluation Laboratory and Riker Laboratories, Inc. Experiment Number: 0681TR0110, December 1981.

Gortner, E. G. 1982. Oral Teratology Study of T 3141CoC in Rabbits. Safety Evaluation Laboratory and Riker Laboratories, Inc. Experiment Number: 0681TB0398, February 1982.

Griffith, F. D., and Long J. E. 1980. Animal toxicity studies with ammonium perfluorooctanoate. Am. Ind. Hyg. Assoc. J. 41( 8): 576 583.

Hanhijarvi, H., Ophaug, R. H., and Singer, L. 1982. The sex related difference in perfluorooctanoate excretion in the rat. Proc. Soc. Exp. Biol. Med. 171: 50 55.

Hanhijarvi, H., M. Ylinen, A. Kojo, and V. Kosma. 1987. Elimination and toxicity of perfluorooctanoic acid during subchronic administration in the Wistar rat. Pharmacol. Toxicol. 61: 66 68.

Hanhijarvi, H. et al. 1988. A proposed species difference in the renal excretion of perfluorooctanoic acid in the beagle dog and rat In: Beynen, A. C. and H. A. Solleveld ( Eds). New Developments in Biosciences: Their Implications for Laboratory Animal Science. Martinus Nijhoff Publishers. Dordrecht, Netherlands.

Hansch, C and Leo, A ( Eds.). 1979. Chapter IV, The Fragment Method of Calculated Partition Coefficients. Substituent Constants for Correlation Analysis and Chemistry and Biology. John Wiley and Sons, Inc.

Hansen K. J., H. O. Johnson, J. S. Eldridge, J. L. Butenhoff, and L. A. Dick, 2002, Quantitative characterization of trace levels of PFOS and PFOA in the Tennessee River. Environ. Sci. Technol. 2002, 36, 1681 1685.

Hatfield, T. 2001. Screening Studies on the Aqueous Photolytic Degradation of Perfluorooctanoic Acid ( PFOA). 3M Environmental Laboratory. Lab request number E00 2192. St. Paul, MN.

Henwood, S. 1997. 5 Daily Dose Oral Toxicity Study with T 6669 in Rats. Corning Hazleton, Inc., Madison, WI. Laboratory Project Identification: CHW 6329 197. 3M Company, St. Paul, 95 MN.

Heuvel, J. P. V. et al. 1991. Tissue distribution, metabolism, and elimination of perfluorooctanoic acid in male and female rats. J. Biochem. Toxicology. 6( 2): 83 92.

Heuvel, J. P. V. et al. 1992. Renal excretion of perfluorooctanoic acid in male rats: inhibitory effect of testosterone. J. Biochem. Toxicology. 7( 1): 31 36.

Heuvel, J. P. V. et al. 1992. Covalent Binding of Perfluorinated Fatty Acids to Proteins in the Plasma, Liver, and Testes of Rats. Chem Biol Interactions. 82: 317 328.

Howell R. D., Johnson, J. D., Drake, J. B., Youngblom, R. D. 1995. Assessment of the Bioaccumulative Properties of Ammonium Perfluorooctanoate: Static. 3M Technical Report. May 31.

IARC, 1995. Peroxisome Proliferation and its Role in Carcinogenesis. IARC Technical Report No. 24. International Agency for Research on Cancer, Lyon, France.

Ikeda, T., Aiba, K., Fukuda, K. and Tanaka, M. 1985. The induction of peroxisome proliferation in rat liver by perfluorinated fatty acids, metabolically inert derivatives of fatty acids. J. Biochem. 98: 475 482 Industrial Bio Test Laboratories, Inc. 1977a. Report to 3M Company: 28 Day Oral Toxicity Study with FC 143 in Albino Mice. IBT No. 8532 10655.

Industrial Bio Test Laboratories, Inc. 1977b. Report to 3M Company: 28 Day Oral Toxicity Study with FC 143 in Albino Rats. IBT No. 8532 10654.

Johnson, J. D. 1995a. Final Report, Analytical Study, Single Dose Intravenous Pharmacokinetic Study of T 6067 in Rabbits. Study Number: AMDT 120694.1. 3M Environmental Technology & Services, St. Paul, MN.

Johnson, J. D. 1995b. Final Report, Analytical Study, Single Dose Absorption/ Toxicity Study of T 6067, T 6068, and T 6069 in Rabbits. Study Number: AMDT 011095.1. 3M Environmental Technology & Services, St. Paul, MN.

Johnson, J. D., Gibson, S. J., and Ober, R. E. 1984. Cholestyramine enhanced fecal elimination of carbon 14 in rats after administration of ammonium [ 14C] perfluorooctanoate or potassium [ 14C] perfluorooctanesulfonate. Fund. Appl. Toxicol. 4: 972 976.

Kachanova, Z. P.; Koslov, J. N. Zh. Fiz. Khim. 1973, Vol. 47, p. 2107.

Kawashima, Y., Uy Yu, N. and Kozuka, H. 1989. Sex related difference in the inductions by perfluoro octanoic acid of peroxisome   oxidation, microsomal 1 acylglycerolphosphocholine 96 acyltransferase and cytosolic long chain acyl CoA hydrolase in rat liver. Biochem. J. 261: 595 600.

Kennedy, G. L. 1985. Dermal toxicity of ammonium perfluorooctanoate. Toxicol. Appl. Pharmacol. 81( 2): 348 355.

Kennedy, G. L. 1987. Increase in mouse liver weight following feeding of ammonium perfluorooctanoate and related fluorochemicals. Toxicol. Lett. 39: 295 300.

Kennedy, G. L., Hall, G. T., Brittelli, M. R., Barnes, J. R., and Chen, H. C. 1986. Inhalation toxicity of ammonium perfluorooctanoate. Food Chem. Toxicol. 24( 12): 1325 1329.

Kidde, 2000. Kidde Fire Fighting, Press Release Re: 3M Withdraws from Fire Fighting Foam Manufacture, May 30, 2000.

Kirk Othmer, 1994. " Fluorinated Higher Carboxylic Acids" under " Fluorine Compounds, Organic ( Higher Acids)" in Kirk Othmer Encyclopedia of Chemical Technology, 4th ed., Vol. 11, pp. 551 558.

Kudo, N., Katakura, M., Sato, Y., Kawashima, Y. 2002. Sex hormone regulated renal transport of perfluorooctanoic acid. Chem. Biol. Interact. 139: 301 316.

Kurume Laboratory, 2001. Bioaccumulation test of Perfluoroalkylcarboxylic acid ( C= 7 13) [ This test is performed using Perfluorooctanoic acid ( Test substance number K 1519)] in carp. Test No. 51519., Chemicals Evaluation and Research Institute, Japan., December 18, 2001, pages 1 26.

Lawlor, T. E. 1996. Mutagenicity Test with T 6564 in the Salmonella   Escherichia Coli/ Mammalian microsome Reverse Mutation Assay with a Confirmatory Assay. Corning Hazleton Inc. Final Report. CHV Study No: 17750 0 409R. September 13.

Lawlor, T. 1995. Mutagenicity test with T 6342 in the Salmonella Escherichia coli/ mammalian microsome reverse mutation assay. Laboratory Number: 17073 0 409. Corning Hazleton Inc., Vienna, VA. 3M Company. St. Paul, MN.

Lines, D. and Sutcliffe, H., Journal of Fluorine Chemistry, 25, 505  512 ( 1984).

Liu, R. C. M., Hurtt, M. E., Cook, J. C. and Biegel, L. B. 1996. Effect of the peroxisome proliferator, ammonium perfluorooctanoate ( C8), on hepatic aromatase activity in adult male Crl: CD BR ( CD) rats. Fund. Appl. Toxicol. 30: 220 228.

Liu, S. C., Sanfilippo, B., Perroteau, I., Derynck, R., Salomon, D. S. and Kidwell, W. R. 1987. Expression of transforming growth factor   ( TGF ) in differentiated rat mammary tumors: estrogen induction of TGF  production. Mol. Endocrinol. 1: 683 692. 97 Longnecker, D. S. 1987. Interface between adaptive and neoplatic growth in the pancreas. Gut, 28: 253 258.

LPSD, 2000. www. lpsd. zzzip. net/ projects. htm Lunak, S.; Sediak, P. Photoinitiated Reactions of Hydrogen Peroxide in the Liquid Phase. J. Photochem. Photobiol. A.: Chem. 1992, Vol. 68, pp. 1 33.

Mendel, A. 1978. Soil Thin Layer Chromatography   FC 95, FC 143, FM 3422. Excerpt from 3M Technical Notebook. October 13, 1978. Number 48277, p30. Project Number 9970612600.

Metrick, M. and Marias, A. J. 1977. 28 Day Oral Toxicity Study with FC 143 in Albino Rats, Final Report, Industrial Bio Test Laboratories, Inc. Study No. 8532 10654, 3M Reference No. T 1742CoC, Lot 269, September 29, 1977.

Moody C. A. and J. A. Field, 1999. Determination of Perfluorocarboxylates in Groundwater Impacted by Fire fighting Activity. Environ. Sci. Technol. 1999, 33, 2800 2806.

Moody, C. A., J. W. Martin, W. C. Kwan, D. C. G. Muir, S. A. Mabury. 2002. Monitoring Perfluorinated Surfactants in Biota and Surface Water Samples Following an Accidental Release of Fire Fighting Foam into Etobiocoke Creek. Environ. Sci. Technol. 2002, 36( 4), 545 551.

Murli, H. 1995. Mutagenicity test on T 6342 in an mouse micronucleus assay. Corning Hazleton Inc., Vienna, VA. 3M Company. St. Paul, MN.

Murli, H. 1996a. Mutagenicity test on T 6564 in an mouse micronucleus assay. Study number 17750 0 455. 3M Company, St. Paul, MN.

Murli, H. 1996b. Mutagenicity Test on T 6564 Measuring Chromosomal Aberrations in Chinese Hamster Ovary ( CHO) Cells: with a Confirmatory Assay with Multiple Harvests. Final Report. Corning Hazleton Inc. CHV Study No.: 17750 0 437CO. September 16.

Murli, H. 1996c. Mutagenicity Test on T 6342, Measuring Chromosomal Aberrations in Whole Blood Lymphocytes With a Confirmatory Assay With Multiple Harvests. Corning Hazelton, Inc. ( CHV). Vienna, VA. CHV Study No.: 17073 0 449CO.

Murli, H. 1996d. Mutagenicity Test on T 6342, Measuring Chromosomal Aberrations in Chinese Hamster Ovary ( CHO) Cells: with a Confirmatory Assay with Multiple Harvests. Corning Hazelton, Inc. ( CHV). Vienna, VA. CHV Study No.: 17073 0 437CO.

Napoli M., Fraccaro C., Scipioni A., and Armelli R. 1984. Thermal Decomposition of Perfluoroalkanesulfonyl Fluorides: The Pyrolysis of Perfluoro n octane 1 sulfonyl Fluoride. Journal of Fluorine Chemistry, 24 , 377 385. 98 Nilsson, R., Beije, B., Preat, V., Erixon, K. and Ramel, C. 1991. On the mechanism of the hepatocarcinogenicity of peroxisome proliferators. Chem. Biol. Interact. 78: 235 250.

NOTOX. 2000. Evaluation of the Ability of T 7524 to Induce Chromosome Aberrations in Cultured Peripheral Human Lymphocytes. NOTOX Project Number 292062. Hertogenbosch, The Netherlands.

NOTOX. 2001. Assessment of Contact Hypersensitivity to T 7524 in the Albino Guinea Pig ( Maximisation Test). NOTOX Project number 292027. Hertogenbosch, The Netherlands.

Nubbe, M. E.; Adams, V. D.; Moore, W. M. The Direct and Sensitized Photo oxidation of Hexachlorocyclopentadiene, Wat. Res. 1995, Vol. 29, No. 5, pp1287 1293.

Obourn, J. D., Frame. S. R., Bell, R. H. Jr., Longnecker, D. S., Elliott, G. S. and Cook, J. C. 1997. Mechanisms for the pancreatic oncogenic effects of the peroxisome proliferator Wyeth 14,643. Toxicol. Appl. Pharmacol. 145: 425 436.

OECD Guideline for Testing of Chemicals, Phototransformation of Chemicals in Water Direct and Indirect Photolysis, ( Draft Document); OECD, 2000, pp1 59.

Ogata, Y.; Tomizawa, K.; Furuta, K. Chemistry of Peroxides, in S. Patai ( ed), The Chemistry of Peroxides 1983, p. 720 Olsen, G. W. et al. 1998a. An Epidemiologic Investigation of Reproductive Hormones in Men with Occupational Exposure to Perfluorooctanoic Acid. JOEM. 40( 7): 614 622.

Olsen, G. W., et al. 1998b. 3M Final Report: An epidemiologic investigation of plasma cholecystokinin, hepatic function and serum perfluorooctanoic acid levels in production workers. 3M Company, St. Paul. Sept 4.

Olsen, G. W., Burris, J. M., Burlew, M. M., Mandel, J. H. 2000. Plasma cholecystokinin and hepatic enzymes, cholesterol and lipoproteins in ammonium perfluorooctanoate production workers. Drug Chem Tox. 23( 4): 603 620.

Olsen, GW, Logan, PW, Simpson, CA, Burris, JM, Burlew, MM, Lundberg, JK, Mandel, JH. 2001a. Descriptive summary of serum fluorochemical levels among employee participants of the year 2000 Decatur fluorochemical medical surveillance program. Final Report. March 19, 2001.

Olsen, GW, Schmickler, M, Tierens, JM, Logan, PW, Burris, JM, Burlew, MM, Lundberg, JK, Mandel, JH. 2001b. Descriptive summary of serum fluorochemical levels among employee participants of the year 2000 Antwerp fluorochemical medical surveillance program. Final Report. March 19, 2001. 99 Olsen, GW, Madsen, DC, Burris, JM, Mandel, JH. 2001c. Descriptive summary of serum fluorochemical levels among 236 building employees. Final Report. March 19, 2001.

Olsen, GW, Hansen, Clemen, LA, Burris, JM, Mandel, JH. 2001d. Identification of Fluorochemicals in Human Tissue. Final Report. Epidemiology, 220 3W 05, Medical Department, 3M Company, St. Paul, MN 55144.

Olsen GW, Burlew MM, Burris JM, Mandel JH. 2001e. A cross sectional analysis of serum perfluorooctanesulfonate ( PFOS) and perfluorooctanoate ( PFOA) in relation to clinical chemistry, thyroid hormone, hematology and urinalysis results from male and female employee participants of the 2000 Antwerp and Decatur fluorochemical medical surveillance program. Final report. 3M Medical Department.

Olsen, G. W., Burlew, M. M, Burris, J. M., Mandel, J. H. 2001f. A Longitudinal Analysis of Serum Perfluorooctanesulfonate ( PFOS) and Perfluorooctanoate ( PFOA) Levels in Relation to Lipid and Hepatic Clinical Chemistry Test Results from Male Employee Participants of the 1994/ 95, 1997, and 2000 Fluorochemical Medical Surveillance Program. 3M Final Report.

Olsen, GW, Burlew, MM, Hocking, BB, Skratt, JC, Burris, JM, Mandel, JH. 2001g. An epidemiologic analysis of episodes of care of 3M Decatur chemical and film plant employees, 1993 1998. Final Report. May 18, 2001.

Olsen, GW, Burris, JM, Lundberg, JK, Hansen, KJ, Mandel, JH, Zobel, LR. 2002a. Identification of fluorochemicals in human sera. I. American Red Cross adult blood donors. Final report. 3M Medical Department.

Olsen, GW, Burris, JM, Lundberg, JK, Hansen, KJ, Mandel, JH, Zobel, LR. 2002b. Identification of fluorochemicals in human sera. II. Elderly participants of the Adult Changes in Thought Study, Seattle, Washington. Final Report. 3M Medical Department.

Olsen, GW, Burris, JM, Lundberg, JK, Hansen, KJ, Mandel, JH, Zobel, LR. 2002c. Identification of fluorochemicals in human sera. III. Pediatric participants in a Group A Streptococci clinical trial investigation. Final Report. 3M Medical Department.

O'Malley, K. D., and Ebbens, K. L. 1981. Repeat Application 28 Day Percutaneous Absorption Study with T 2618CoC in Albino Rabbits. Riker Laboratories, St. Paul, MN.

Ophaug, R. H. and L. Singer. 1980. Metabolic Handling of Perfluorooctanoic Acid in Rats. Proc Soc Exp Biol Med. 163: 19 23.

Pace Analytical. 1997. Ready Biodegradation of FC 126( BOD/ COD). 3M Company Lab Request No. E1282. Minneapolis, MN. May 29. 100 Pace Analytical. 2001. The 18 Day Aerobic Biodegradation Study of Perfluorooctanesulfonyl Based Chemistries. 3M Company Request, Contract Analytical Project ID: CA097, Minneapolis, MN. February 23.

Palazzolo, M. J. 1993. Thirteen Week Dietary Toxicity Study with T 5180, Ammonium Perfluorooctanoate ( CAS No. 3825 26 1) in Male Rats. Final Report. Laboratory Project Identification HWI 6329 100. Hazleton Wisconsin, Inc.

Pastoor, T. P., Lee, K. P., Perri, M. A. and Gillies, P. J. 1987. Biochemical and morphological studies of ammonium perfluorooctanoate induced hepatomegaly and peroxisome proliferation. Exp. Mol. Pathol. 47: 98 109.

Petritis, 1999. K. Petritis, et al. " Ion pair reversed phase liquid chromatography for determination of polar underivatized amino acids using perfluorinated carboxylic acids as ion pairing agent" in J. Chromatography A, Vol. 833, 1999, pp. 147 155.

Reiner, E. A. 1978. Fate of Fluorochemicals in the Environment. Project Number 9970612613. 3M Company, Environmental Laboratory. July 19.

Reiner, E. A. 1981. 3M Company Environmental Laboratory, St. Paul, Minnesota, Dec. 7.

Renner, 2001. " Growing Concern Over Perfluorinated Chemicals" in Environmental Science and Technology, Vol. 35, Issue 7, pp. 154A 160A, April 1, 2001.

Riker Laboratories, Inc., Safety Evaluation Laboratory. 1979. Repeat Application 28 Day Percutaneous Absorption Study with T 2618CoC in Albino Rabbits. St. Paul, Minnesota. Experiment Number: 09790AB0485.

Riker Laboratories Inc., Safety Evaluation Laboratory. 1983. Primary Skin Irritation Test with T 3371 in Albino Rabbits. St. Paul, MN. Experiment # 0883EB0079.

Sadhu, D. 2002 CHO/ HGPRT forward mutation assay   ISO ( T6.889.7) Toxicon Corporation, Bedford, MA, Toxicon Final Report: 01 7019 G1 Submitted to: 3M, St. Paul Minnesota 55144 1000.

Scrano, L.; Bufo, S. A.; Perucci, P.; Meallier, P.; Mansour, M. Photolysis and Hydrolysis of Rimsulfuron, Pestic. Sci. 1999, Vol. 55, pp. 955 961.

Simister, E. et. al. J. Chem. Soc., Faraday Trans. 88( 20), 3033 41 ( 1992)

Sohlenius, A. K., Anderson, K. and DePierre, J. 1992. The effects of perfluorooctanoic acid on hepatic peroxisome proliferation and related parameters show no sex related differences in mice. Biochem. J. 265: 779 783. 101 Staples, R. E., Burgess, B. A., and Kerns, W. D. 1984. The Embryo Fetal Toxicity and Teratogenic Potential of Ammonium Perfluorooctanoate ( APFO) in the Rat. Fund. Appl. Tox. 4, 429 440.

Takagi, A., Sai, K., Ummemura, T., Hasegawa, R. and Kurokawa, Y. 1991. Short term exposure to the peroxisome proliferators, perfluorooctanoic acid and perfluorodecanoic acid, causes significant increases of 8 hydroxydeoxyguanosine in liver DNA of rats. Cancer Lett. 57: 55 60.

Takagi, A., Sai, K., Ummemura, T., Hasegawa, R. and Kurokawa, Y. 1992. Hepatomegaly is an early biomarker for hepatocarcinogenesis induced by peroxisome proliferators. J. Environ. Toxicol. Pathol. 11: 145 149.

Taylor P., Dellinger B. and Lee C. C., 1990. Development of a Thermal Stability Based Ranking of Hazardous Organic Compound   Incinerability. Environmental Science & Technology, 24, 316 328.

Teerds, K. J., Rommerts, F. G. and Dorrington, J. H. 1990. Immunohistochemical detection of transforming growth factor   in Leydig cell during the development of the rat testis. Molec. Cell Endocrinol. 69: R1 R6.

Thomford, PJ. 2001b. 26 Week Capsule Toxicity Study with Ammonium Perfluorooctanoate ( APFO) in Cynomolgus Monkeys. Study performed by Covance Laboratories Inc., Madison Wisconsin 53704 2592 for APME Ad hoc APFO Toxicology Working Group. Study No. Covance 6329 231, Completion Date December 18, 2001, 463 pp.

Thomford PJ. 2001b. 4 Week Capsule Toxicity Study with Ammonium Perfluorooctanoate ( APFO) in Cynomolgus Monkeys. Study performed by Covance Laboratories Inc., Madison Wisconsin 53704 2592 for APME Ad hoc APFO Toxicology Working Group. Study No. Covance 6329 230, Completion Date December 18, 2001, 159 pp.

Todd, J. W. 1979. FC 143 Photolysis Study Using Simulated Sunlight. Project 9776750202, 3M Company Technical Report No. 002. February 2.

T. R. Wilbury Laboratories, Inc. 1995. Growth and Reproduction Toxicity Test with N2803 3 and the Freshwater Alga, Selenastrum capricornutum. Marblehead, MA. Study number B93 TH.

T. R. Wilbury Laboratories, Inc. 1996a. Acute toxicity of N2803 3 to the Fathead Minnow, Pimephales promelas. Marblehead, MA. Study number 891 TH.

T. R. Wilbury Laboratories, Inc. 1996b. Acute Toxicity of N2803 3 to the Daphnid, Daphnia magna. Marblehead, MA. Study number 892 TH.

Tsang W., Burgess D. R., and Babushok V., 1998. On the Incinerability of Highly Fluorinated Organic Compounds. Combustion Science & Technology, 1998, 139, 385 402. 102 Ubel FA, Sorenson SD, Roach DE. 1980 Health status of plant workers exposed to fluorochemicals a preliminary report. Am Ind Hyg Assoc J 41: 584 589.

USEPA 1998 Fate, Transport and Transformation Test Guidelines, OPPTS 835.5270 Indirect Photolysis Screening Test; EPA712 C 98 099; United States Environmental Protection Agency, U. S. Government Printing Office: Washington, DC, 1998, pp1 22.

USEPA 1998. Fate, Transport and Transformation Test Guidelines: 835.2110: Hydrolysis as a Function of pH; EPA712 C 98 057; United States Environmental Protection Agency, U. S. Government Printing Office: Washington, DC.

USEPA 2002a. Memorandum from Dr. Ralph Cooper, NHEERL, to Dr. Jennifer Seed, dated October 2, 2002.

USEPA 2002b. Memorandum from Dr. Elizabeth Margosches to Dr. Katherine Anitole, dated October 21, 2002.

Vanden Heuvel, J. P., Davis, J. W., Sommers, R., and Peterson, R. E. 1992. Renal excretion of perfluorooctanoic acid in male rats: Inhibitory effect of testosterone. J. Biochem. Toxicol. 7( 1): 31 36.

Vanden Heuvel, J. P., Kuslikis, B. I., and Peterson, R. E. 1991a. Covalent binding of perfluorinated fatty acids to proteins in the plasma, liver and testes of rats. Chem. Biol. Interact. 82: 317 328.

Vanden Heuvel, J. P., Kuslikis, B. I., Van Rafelghem, M. L. and Peterson, R. E. 1991b. Tissue distribution, metabolism, and elimination of perfluorooctanoic acid in male and female rats. J. Biochem. Toxicol. 6( 2): 83 92.

Vraspir, G. A., Mendel, Arthur. 1979. Analysis for Fluorochemicals in Bluegill Fish. Project 9970612600: Fate of Fluorochemicals. 3M Technical Report Report Number 14. May 1.

Ward, T., Nevius, J. and R. Boeri. 1996a. Acute toxicity of FC 1015 to the fathead minnow, Pimephales promelas. T. R. Wilbury Laboratories, Inc. Lab Request number P1624. 3M Company, St. Paul, MN.

Ward, T., Nevius, J. and R. Boeri. 1996b. Growth and reproduction toxicity test with FC 1015 and the freshwater alga, Selenastrum capricornutum. T. R. Wilbury Laboratories, Inc. Lab Request number P1624. 3M Company, St. Paul, MN.

Ward, T., Nevius, J., and R. Boeri. 1996c. Acute toxicity of FC 1015 to Daphnid, Daphnia magna. T. R. Wilbury Laboratories, Inc. Lab request number P1624. 3M Company, St. Paul, MN. 103 Welsh, S. K. 1978. Technical Report Summary  Adsorption of FC 95 and FC 143 on soil. Environmental Laboratory. 3M Company Project 9970612633: Fate of Fluorochemicals, Report Number 1. St. Paul, MN. February 27.

Yager, J. D. Jr. and Yager, R. 1980. Oral contraceptive steroids as promoters of hepatocarcinogenesis in female Sprague Dawley rats. Cancer Res. 40: 3680 3685.

Yang, Q., Xie, Y., and Depierre, W. 2000. Effects of peroxisome proliferators in the thymus of spleen and mice. Clin. Exp. Immunol. 122: 219 226.

Yang, Q., Xie, Y., Ericksson, A. M., Nelson, B. D., and DePierre, J. W. 2001. Further evidence for the involvement of inhibition of cell proliferation and development in thymic and splenic atrophy induced by the peroxisome proliferator perfluoroctanoic acid in mice. Biochem. Pharmacol. 62: 1133 1140.

Yang, Q., Xie, Y., Alexson, S. E. H., Nelson, B. D., and DePierre, J. W. 2002a. Involvement of the peroxisome proliferator activated receptor alpha in the immunomodulation caused by peroxisome proliferators in mice. Biochem. Pharmacol. 63: 1893 1900.

Yang, Q., Abedi Valugerdi, M., Xie, Y. Zhao, X., Moller, G., Nelson, B. D., and DePierre, J. W. 2002b. Potent suppression of the adaptive immune response in mice upon dietary exposure to the potent peroxisome proliferator, perfluorooctanoic acid. International Immunopharmacology 2, 389 397.

Ylinen, M., Hanhijarvi, H., Jaakonaho, I., and Peura, P. 1989. Stimulation by estradiol of the urinary excretion of perfluorooctanoic acid in the male rat. Pharmacol. Toxicol. 65: 274 277.

Ylinen, M., Kojo, A., Hanhijdrvi, H. and Peura, P. 1990. Disposition of perfluorooctanoic acid in the rat after single and subchronic administration. Bull. Environ. Contam. Toxicol. 44: 46 53.

York, R. G. 2002. Oral ( Gavage) Two Generation ( One Litter Per Generation) Reproduction Study of Ammonium Perfluorooctanoic ( APFO) in Rats. Argus Research Laboratories, Inc. Protocol Number: 418 020, Sponsor Study Number: T 6889.6, March 26, 2002