King County Environmental Laboratory Standard Operating Procedure MICROBIOLOGY, FECAL COLIFORMS IN ENVIRONMENTAL WATER BY MF PAGE 1 OF 11 PAGES Date approved: Revision number: 0 File name: DCTM_ ARP. doc SOP #: 05 03 001 000 STANDARD OPERATING PROCEDURE for Fecal Coliforms in Environmental Water by Membrane Filtration SOP # 05 03 001 000 Date of Implementation:

Supersedes SOP: Microbiology QA Manual Section 6.1 Approved by:

Supervisor: _______________________________ Date: ________________

QA Officer: _______________________________ Date: ________________

King County Environmental Laboratory 322 W. Ewing Street Seattle WA 98119 1507 King County Environmental Laboratory Standard Operating Procedure MICROBIOLOGY, FECAL COLIFORMS IN ENVIRONMENTAL WATER BY MF PAGE 2 OF 11 PAGES Date approved: Revision number: 0 File name: DCTM_ ARP. doc SOP #: 05 03 001 000 1.0 Scope and Application The fecal coliform group includes two genera of bacteria and is used as a measurement of fecal pollution. The bacterial standards for recreational waters are based upon the concentration of fecal coliforms. The analysis of environmental water for fecal coliforms using Membrane Filtration is described in this SOP.

2.0 Method Summary Appropriate dilutions, if necessary, are prepared using APHA buffered water as the diluent. The sample and it's dilutions are filtered through a sterile 0.45 µ m nitrocellulose membrane. The membrane is then applied to an mFC agar plate. After incubation at 44.5 ± 0.2oC for 24 ± 2 hours, all colonies with a characteristic blue color are counted. The number of colonies per 100 mL of sample is calculated based on the colonies counted and the dilutions used per plate.

3.0 Definitions Coliform: A grouping of several genera of bacteria of the family Enterobactericaceae that are identified by their ability to ferment lactose under specified conditions.

Fecal Coliform: Facultative anaerobic, Gram negative, non spore forming rod shaped bacteria that develops blue colonies on mFC agar within 24+ 2hr h at 44.5 + 0.2 ° C.

Membrane Filtration ( MF): The technique used to determine the number of colonies in a sample using filtration through a nitrocellulose filter of nominal 0.45 µ m pore size and incubation on an agar plate or a liquid substrate pad.

Most Probable Number ( MPN): The density of the bacteria in a sample calculated using probability formulas and the observations of replicate tubes and multiple dilutions.

4.0 Interferences Algae, particulates, colloids, solids or other material, as identified by sample turbidity, may interfere with MF analyses. Samples affected by these interferences should be tested with the MPN technique. Non sterile equipment, materials or work environment may also interfere with the MF technique.

5.0 Safety and Hazardous Materials Management Each sample to be tested for fecal coliforms should be considered a potential biohazard or chemical hazard. Aerosols may be generated in handling the samples; exercise protective King County Environmental Laboratory Standard Operating Procedure MICROBIOLOGY, FECAL COLIFORMS IN ENVIRONMENTAL WATER BY MF PAGE 3 OF 11 PAGES Date approved: Revision number: 0 File name: DCTM_ ARP. doc SOP #: 05 03 001 000 measures. Protective equipment such as lab coats should be worn whenever handling the samples. Latex or nitrile gloves may also be worn as a protective barrier. Used plates and contaminated disposable supplies must be stored in containers clearly labeled as biohazardous until sterilized by autoclaving. Autoclaved solid waste may then be discarded in a solid waste container. Liquids not identified as hazardous waste may be discarded in a sanitary drain.

6.0 Sample Collection, Containers, Preservation and Storage Polypropylene ( PP) or high density polyethylene ( HDPE) bottles must be autoclaved and identified as sterile with autoclave tape before being used for sample collection. Containers must be filled such that a minimum of 1 inch of headspace is present. NPDES water samples must be preserved with sterile sodium thiosulfate solution if residual chlorine is present or expected, stored at 4oC and analyzed within 6 hours of collection. Non NPDES water samples must be preserved with sterile sodium thiosulfate solution if residual chlorine is present or expected, stored at 4 oC and analyzed within 24 hours of collection.

7.0 Apparatus and Equipment 44.5 ± 0.2 oC water bath ( or equivalent incubator) Sterile 100 ml graduated cylinders Sterile filter funnels Manifold UV sterilizer for filter funnel units Bunsen burner or other flame source Vacuum source Pipetter Wescodyne or other disinfectant disposal bucket Non serrated forceps Sterile rinse water dispenser 8.0 Preventative Maintenance All reusable equipment that may have been in contact with the samples must be cleaned and sterilized between batches.

9.0 Reagents, Media and Consumables Sterile APHA phosphate buffered dilution water Sterile 18mL APHA phosphate buffered dilution tubes Sterile 1, 5, 10 and 25 mL pipettes or pipette tips (< 2.5% tolerance) 70% alcohol ( ethanol or isopropyl alcohol) King County Environmental Laboratory Standard Operating Procedure MICROBIOLOGY, FECAL COLIFORMS IN ENVIRONMENTAL WATER BY MF PAGE 4 OF 11 PAGES Date approved: Revision number: 0 File name: DCTM_ ARP. doc SOP #: 05 03 001 000 Sterile 0.45 µ m membrane filters, nitrocellulose with gridlines, 47mm diameter Whirl pak

bags Sterile mFC medium in sterile Petri dishes ( See SOP# 05 01 014 000 for media preparation)

10.0 Procedures 10.1 Preparation Determine what volumes need to be run for the type of water being analyzed. Consult Appendix A for the suggested analysis volumes for different types of water. The samples are workgrouped in LIMS such that the lab ID# s (" L" numbers), parameters, dilutions and associated QC samples are defined for a particular batch. Once workgrouped, LIMS can generate a label to be placed on each plate of medium for each specific dilution. The plate must be associated with a sample identifier, the volume of sample tested, the date tested, and the type of test. An electronic login sheet is also generated by LIMS, which records the sample, product, and date workgrouped. The login sheet is used to record the date and time a sample was received and processed, as well as the analysts performing these procedures. For specific information on the use of LIMS for labeling and documenting sample preparation, see " LIMS USER'S MANUAL FOR THE MICROBIOLOGY SECTION" ( SOP # 05 01 001 000).

If needed, prepare dilutions of the sample. To prepare a 1: 10 dilution, add 2 mL of the sample to an 18 mL sterile APHA buffered water dilution tube. Subsequent dilutions are made in the same manner. Alternate volumes may be used to accommodate the volume of sample available for testing.

10.2 Filtration Set up the vacuum manifold with an inline filter between the vacuum valve and the collection vessel. Place the filtration units on the vacuum manifold and close all manifold valves. Disinfect the non serrated forceps by placing the tips in 70% alcohol solution for 90 seconds. Remove residual alcohol by placing the tips of the forceps briefly in the flame of the Bunsen burner. With the sterile forceps, center a sterile, 0.45 µ m membrane filter on the base of each filter unit, and then place the funnel portion of the unit on the base. Measure the amount of water to be filtered with a graduated cylinder if the volume is > 30 ml, or use a pipette if the volume is   30 ml. If the volume filtered is < 30 ml, add a 30 50 ml reservoir of APHA buffered water to the filter funnel before adding the sample. A 100 ml sample volume may be measured by using the markings on the funnel itself. Add the sample to the filter funnel and turn on the vacuum. Once the sample is filtered completely, thoroughly rinse the funnel with APHA buffered water, making sure that the sides of the funnel are washed down. Use a minimum of approximately 30 ml for each rinse. Once all of the rinse water is filtered through, turn off the vacuum by closing the manifold valve.

Remove the membrane filter from the filtering unit by grasping the edge of the filter with the sterile forceps, and then place the filter onto mFC agar plates previously prepared. Try to " roll" the filter onto the agar such that the filter is in complete contact with the agar King County Environmental Laboratory Standard Operating Procedure MICROBIOLOGY, FECAL COLIFORMS IN ENVIRONMENTAL WATER BY MF PAGE 5 OF 11 PAGES Date approved: Revision number: 0 File name: DCTM_ ARP. doc SOP #: 05 03 001 000 surface. Check for air bubbles underneath the filter; if there are air bubbles, try lifting the filter by the edge and placing it on the agar again. If this does not work, then touch the edge of the filter with the forceps in such a way as to make it sit in contact with the medium. Do not touch the area of the filter that the sample has gone through only handle the very outside edge. Repeat the disinfection and flaming of the forceps between each sample. UV sterilize funnels for 90 seconds between samples.

10.3 Incubation Plates should be transferred to the water bath ( or incubator) within 30 minutes of filtration. Place the plates in Whirl pak bags and close the bags by tightly " whirling" the bag. Bend the metal closures in such a way as to prevent the bag from uncoiling itself and to prevent the tips of the metal closures from puncturing the bag. Label each bag with the time and date. Submerge the closed bags in a 44.5 ± 0.2 oC water bath for 24 ± 2 hrs. Incubators other than water baths do not require Whirl bags. For the automatic incubators, the time entered and starting temperature is recorded, along with the final temperature at the end of the incubation period.

10.4 Counting Colonies Count with the aid of at least 1  2x magnification with the aid of a fluorescent light. Fecal coliform colonies are those which appear blue, either completely or at the margin or center of the colony. Colonies vary from a deep blue color to a pale blue, and may have a gray to cream colored center with blue on the outside. Size of the colony can vary from 1 4 mm. Do not count blue pin point colonies (< 1mm) unless confirmed. Colonies that are brown, cream, white, or any other color that does not have any blue are not counted as fecal coliforms. False positives may be caused by non coliform bacteria that can ferment lactose under the conditions of the test. Confirmation of colonies detected in field samples is done at the discretion of the analyst. Colonies typically confirmed would have inconsistent color or halo patterns. Confirmations are done on a monthly basis on QC samples as per section 11.5.

10.5 Verification Procedure Questionable colonies, especially colonies from a sample exhibiting atypical morphology, should be verified. Verify by inoculating a Lauryl Tryptose Broth ( LTB) tube for each questionable colony to be verified and incubate at 35 + 0.5 ° C for 24 to 48 hrs. Tubes with turbidity and gas production in LTB within 48 hrs are then transferred to EC broth and incubated at 44.5 + 0.2 ° C for 24 + 2 hours. Turbidity and gas production in EC broth confirms a fecal coliform.

10.6 Data Entry and Qualifiers ( see LIMS Data Entry SOP) Manually enter into LIMS the number of fecal coliform colonies counted into the " num" value field for each sample and dilution that was workgrouped. A zero value should be entered into the " num" value field only if no characteristic colonies are observed. During manual data entry, qualifiers are entered into the qualifier field in LIMS according to the following conditions: King County Environmental Laboratory Standard Operating Procedure MICROBIOLOGY, FECAL COLIFORMS IN ENVIRONMENTAL WATER BY MF PAGE 6 OF 11 PAGES Date approved: Revision number: 0 File name: DCTM_ ARP. doc SOP #: 05 03 001 000   If unable to count the colonies due to confluent growth, use a C and do not enter a " num" value.   If the number of target colonies slightly exceeds 60 without confluent growth, or there is background or confluent growth but the analyst believes colony crowding is not adversely affecting a reliable estimate, enter a number value and qualify it with a C.   If the plates exceed the maximum countable range, an estimate can be taken. For estimates of plates with 3 to 10 target colonies per square, count 10 squares and obtain the average count per square. For those with 10 to 20 target colonies per square, count 5 squares and obtain the average count per square. Multiply the average count per square by 100 to obtain the plate count, and then report that number with an E qualifier. ( Standard Methods 19th ed., 9 33)   If the number of target colonies exceeds the countable range and no reliable estimate is available, use TNTC. Once the data is approved, TNTC will be converted to >### ( the maximum number possible to count, given the volume tested) by LIMS.   Other general use qualifiers may also be used for microbiology data ( see Appendix B of the KCEL QA Manual).

10.7 Calculating Results Results are entered and calculated using specific programs in Seedpak in LIMS. The LIMS program will calculate the colony count per 100 mL for each sample by using the " batching" method. This method calculates by summing the colony counts of all dilutions where a " num" value was entered and dividing by the total volume of sample filtered. The adjusted count is calculated by multiplying the resultant by 100. See Appendix B.

11.0 QA/ QC Requirements Routine QC analyses for Microbiology include both those procedures used to insure the quality of each batch of media and containers and those procedures used to monitor method performance of each sample analysis batch or analysis session. A sample analysis batch should not exceed 20 samples of the same matrix which are all prepared and analyzed together, using the same reagents, media, and equipment. An analysis session consists of one or more batches done within a working day.

Each batch of media must be tested to confirm pH, sterility, and positive and negative performance characteristics. These specific QC procedures are defined in SOP( s) that describe the preparation and QC checks of mFC media ( See SOP# 05 01 014 000 for media preparation). After washing and sterilization, each batch of containers used for collection of samples must be evaluated for appropriate pH, soap residue, and sterility.

11.1 Lab Duplicates Lab duplicates are prepared for each matrix type at a frequency of 1 per batch or 5%, whichever is more frequent. For MF, the acceptance limits are based on the precision criterion calculated from the duplicates of the previous 15 duplicate pairs ( see Standard King County Environmental Laboratory Standard Operating Procedure MICROBIOLOGY, FECAL COLIFORMS IN ENVIRONMENTAL WATER BY MF PAGE 7 OF 11 PAGES Date approved: Revision number: 0 File name: DCTM_ ARP. doc SOP #: 05 03 001 000 Methods, 19th Edition. p. 9 9). These acceptance limits are updated automatically by LIMS whenever duplicate sample results are entered. If the 15 previous duplicate pairs are unavailable, a senior analyst and/ or supervisor must evaluate the duplicate value.

11.2 Negative Control A negative control is prepared at a frequency of 1 per session. The negative control is a suspension of Proteus sp. or Enterobacter sp. The negative control should show an appropriate qualitative response for the test organism and should not be identified as containing fecal coliforms.

11.3 Positive Control A positive control is prepared at a frequency of 1 per session. The positive control is a suspension of E. coli or Klebsiella sp. The positive control should show an appropriate qualitative response for the test organism and should be identified as containing fecal coliforms.

11.4 System Controls For the MF procedure, both a " pre" and a " post" filtration blank are prepared on the least specific media being used that day at a frequency of 1 set per session. The filtration blanks challenge the same batch of APHA water ( 100ml) used to dilute and analyze the samples. These system controls are considered acceptable if the target organism for the media is not detected. If the " pre" and " post" filtration blanks show positive results by the leastspecific method, the controls must be evaluated by each of the more specific methods.

11.5 Verification A monthly sample of Ship Canal water, a known source of fecal coliforms, is tested to verify that these methods are performing appropriately for actual environmental samples. Counting of the plates are done by 3 separate microbiologists. The Relative Percent Difference within analyst and between analyst must be   5% and 10%, respectively. Ten colonies identified as fecal coliforms are confirmed by LTB and then EC broth tubes. At least 80% of each must be positive. Results are documented in the QC logbook. Routinely performing confirmations on the Ship Canal samples helps insure that a minimum percentage of positive results are confirmed each month by multiple analysts.

11.6 Corrective Actions Corrective actions must be taken whenever a QC failure occurs. Unacceptable QC results within a batch or session of analyses require that all samples associated with those batches and/ or session must be reviewed. A decision should be made as to if and how the samples should be qualified. A Corrective Action form should be prepared to document the failure and describe how it was resolved, if possible, and how it could be avoided in the future.

12.0 Reporting, Review and Documentation LIMS raw data sheets are attached to the LIMS hard data review sheets and given to the microbiology project coordinator. The project coordinator reviews the results for acceptability based on compliance with holding times, storage and testing conditions, King County Environmental Laboratory Standard Operating Procedure MICROBIOLOGY, FECAL COLIFORMS IN ENVIRONMENTAL WATER BY MF PAGE 8 OF 11 PAGES Date approved: Revision number: 0 File name: DCTM_ ARP. doc SOP #: 05 03 001 000 positive and negative controls, filter blank results, and conformance with both historical and synoptic data. Appropriate dilution effects and consistency with other parameters are also evaluated. Once the results have been shown to meet all criteria, the project coordinator will approve and post the data to EDS tables in LIMS. All hardcopy data is archived by year and project.

13.0 References American Public Health Association. 1998. Method 9222 D. Fecal Coliform Membrane Filter Procedure. Clesceri, L. S., A. D. Eaton, and A. E. Greenberg ( ed). Standard Methods for the Examination of Water and Wastewater. 20th Edition. American Public Health Association, Washington, DC.

14.0 Training New microbiologists are to be trained at this procedure by first reading the reference method and this SOP, and then observing the method as performed by an experienced analyst. While being directly supervised, the trainee will then perform the method on two separate sets of split samples also performed by an experienced analyst. Once the trainee has produced acceptable QC results and the results of the two sets match those of the experienced analyst, the trainee will be approved as an analyst. King County Environmental Laboratory Standard Operating Procedure MICROBIOLOGY, FECAL COLIFORMS IN ENVIRONMENTAL WATER BY MF PAGE 9 OF 11 PAGES Date approved: Revision number: 0 File name: DCTM_ ARP. doc SOP #: 05 03 001 000 Appendix A Suggested Volumes for Fecal Coliform Analyses by MF Volume Analyzed ( mL) 102 101 100 10 1 10 2 10 3 10 4 10 5 10 6 Project Streams ( routine) x x x Streams ( storms) x x x x Lake Wash. x x Lake Samm. x x Lake Union x x Beaches ( Lakes) x x x Sewage ( raw) x X x x x CSO x x x x X x Trouble Call ( water) x x x x X x Stormwater x x x x x X WP Offshore x Renton Offshore x Beaches * ( Marine) x x * Volumes routinely analyzed are 10, 30 and 100 mL. King County Environmental Laboratory Standard Operating Procedure MICROBIOLOGY, FECAL COLIFORMS IN ENVIRONMENTAL WATER BY MF PAGE 10 OF 11 PAGES Date approved: Revision number: 0 File name: DCTM_ ARP. doc SOP #: 05 03 001 000 Appendix B Calculation of Final MF Results " Batching" Method This process takes the colonies counted for the sample and the volume filtered from each of the selected dilutions and performs the following calculations:

1. Sum all counted colonies. 2. Sum all filtered volumes ( only use the volumes of dilutions which produce a numeric value). Remember to also include the volumes of duplicated dilutions if the sample was run in duplicate. 3. Multiply the sum of the counted colonies (# 1 above) by 100 and divide by the sum of the filtered volumes (# 2 above).

( All Counted Colonies) X 100 ( Total Volume Filtered)

This is the adjusted reported value.

The method detection limit is found by following the instructions under special conditions, number two. Special Conditions:

1. If all dilutions resulted in a count of TNTC:

Divide the maximum countable number for the specific procedure ( see chart below) by the smallest volume filtered. Multiply this result by 100. Place this result in the Qualifier Field as part of the "> #####" where "####" is the result. The Value Field is left blank.

Procedure Maximum Countable Number Fecal Coliform 60 Total Coliform 80 E. coli 80 Enterococci 60 Enterococci ( modified incubation) 60 Fecal Streptococci 60 Heterotrophic Plate Count 200 2. If all dilutions resulted in zero counts:

If the largest volume is   100, divide 1 by the largest volume filtered, and then multiply the resultant by 100 to obtain the count per 100ml. Enter this result as `<####' in the qualifier field. King County Environmental Laboratory Standard Operating Procedure MICROBIOLOGY, FECAL COLIFORMS IN ENVIRONMENTAL WATER BY MF PAGE 11 OF 11 PAGES Date approved: Revision number: 0 File name: DCTM_ ARP. doc SOP #: 05 03 001 000 If the largest volume is < 100, divide 100 by the largest volume filtered to obtain the count per 100 ml. Enter this result as `<####' in the qualifier field.

Significant Digits:

1. If each value used from individual dilutions has only one significant figure ( counts < 10), the final calculated value should only have one sig. fig. 2. If any value from individual dilutions has 2 or 3 sig. figs, the final calculated value should have no more than the smaller of the number of sig figs ( i. e., if the calculation has both 2 and 3 sig fig numbers, the result should be reported with 2 sig figs). 3. Sig. figs should never exceed 3.


[Federal Register Volume 77, Number 171 (Tuesday, September 4, 2012)]
[Notices]
[Pages 53948-53950]
[FR Doc No: 2012-21634]

-----------------------------------------------------------------------

SECURITIES AND EXCHANGE COMMISSION

[Release No. 34-67743; File No. SR-CME-2012-33]

Self-Regulatory Organizations; Chicago Mercantile Exchange Inc.;
Notice of Filing and Immediate Effectiveness of Proposed Rule Change To
Amend the Fee Schedule Applicable to its OTC Interest Rate Swap
Clearing Offering

August 28, 2012.
    Pursuant to Section 19(b)(1) of the Securities Exchange Act of 1934
(``Act'') \1\ and Rule 19b-4 thereunder \2\ notice is hereby given that
on August 17, 2012, Chicago Mercantile Exchange Inc. (``CME'') filed
with the Securities and Exchange Commission (``Commission'') the
proposed rule change as described in Items I, II, and III below, which
Items have been prepared primarily by CME. CME filed the proposed rule
change pursuant to Section 19(b)(3)(A) \3\ of the Act and Rule 19b-
4(f)(2) \4\ thereunder, so that the proposed rule change was effective
upon filing with the Commission. The Commission is publishing this
notice to solicit comments on the proposed rule change from interested
parties.
---------------------------------------------------------------------------

    \1\ 15 U.S.C. 78s(b)(1).
    \2\ 17 CFR 240.19b-4.
    \3\ 15 U.S.C. 78s(b)(3)(A).
    \4\ 17 CFR 240.19b-4(f)(2).
---------------------------------------------------------------------------

I. Self-Regulatory Organization's Statement of Terms of Substance of
the Proposed Rule Change

    CME is proposing to amend the fee schedule that currently applies
to its OTC Interest Rate Swap clearing offering.

II. Self-Regulatory Organization's Statement of Purpose of, and
Statutory Basis for, the Proposed Rule Change

    In its filing with the Commission, CME included statements
concerning the purpose and basis for the proposed rule change and
discussed any comments it received on the proposed rule change. The
text of these statements may be examined at the places specified in
Item IV below. CME has prepared summaries, set forth in sections A, B,
and C below, of the most significant aspects of such statements.

A. Self-Regulatory Organization's Statement of Purpose of, and
Statutory Basis for, the Proposed Rule Change

    CME currently offers clearing for certain OTC Interest Rate Swap
products. This filing proposes to amend the current fee schedule that
applies to CME's OTC Interest Rate Swap (``IRS'') clearing offering.
Specifically, CME will be adding; (i) An optional alternative fee
schedule, (ii) progressive fee tiers for the standard fee schedule, and
(iii) fee waivers for CME OTC IRS clearing member's back-loaded trades.
    Under the new optional alternative fee schedule, house or customer
accounts will be able to elect to be subject to an alternate
transaction fee schedule for OTC IRS that includes certain per ticket
transaction fee and certain monthly charges measured in basis points
annualized on the client's initial margin requirement. Election of the
alternative transaction fee schedule requires notice to CME which must
be given (i) during the firm's onboarding process, or (ii) at least
fifteen (15) days prior to a calendar quarter that the firm elects to
receive the alternative fee schedule.
    The second feature of the proposed changes relates to new
progressive fee tiers. Under these changes, each calendar quarter,
firms may qualify to receive a fixed discount applicable to base OTC
IRS fees for the following calendar quarter on the basis of the USD
equivalent base fees incurred during the current quarter. The discount
applicable to the following calendar quarter will be calculated on a
weighted average basis using the USD equivalent base fees for the
current calendar quarter and certain discount percentages.
Additionally, from September 1, 2012 to December 31, 2013, the proposed
changes would provide for a one-time rebate on current calendar quarter
activity during the first calendar quarter that its weighted average
discount is equal to or greater than 15%.
    Finally, for IRS Clearing Members, the proposed rule changes would
provide for certain fee waivers for back-loaded trades. A backloaded
trade is a trade accepted for clearing where the effective date for the
trade is prior to the date the trade was accepted for clearing. The
proposed changes are related to fees and therefore will become
effective immediately. However, the proposed fee changes will become
operative as of September 1, 2012. CME has also certified the proposed
rule changes that are the subject of this filing to the Commodity
Futures Trading Commission (``CFTC''), in CFTC Submission 12-254.
    The proposed CME rule amendments establish or change a member due,
fee or other charge imposed by CME under Section 19(b)(3)(A)(ii) \5\ of
the Act and Rule 19b-4(f)(2) \6\ thereunder. CME believes that the
proposed rule change is consistent with the requirements of the Act and
the rules and regulations thereunder and, in particular, to Section
17A(b)(3)(D),\7\ in that it provides for the equitable allocation of
reasonable dues, fees and other charges among participants. The
proposed changes apply to all IRS Clearing Members or customers, as
applicable. The modifications should encourage firms to submit
additional volume into the system which should help ensure readiness
and also help build open interest ahead of a regulatory mandate. CME
notes that it operates in a highly competitive market in which market
participants can readily direct business to competing venues.
---------------------------------------------------------------------------

    \5\ 15 U.S.C. 78s(b)(3)(A)(ii).
    \6\ 17 CFR 240.19b-4(f)(2).
    \7\ 15 U.S.C. 78q-1(b)(3)(D).
---------------------------------------------------------------------------

B. Self-Regulatory Organization's Statement on Burden on Competition

    CME does not believe that the proposed rule change will have any
impact, or impose any burden, on competition.

C. Self-Regulatory Organization's Statement on Comments on the Proposed
Rule Change Received From Members, Participants, or Others

    CME has not solicited, and does not intend to solicit, comments
regarding this proposed rule change. CME has not received any
unsolicited written comments from interested parties.

III. Date of Effectiveness of the Proposed Rule Change and Timing for
Commission Action

    The foregoing rule change was filed pursuant to Section 19(b)(3)(A)
\8\ of the Act and Rule 19b-4(f)(2) \9\ thereunder and thus became
effective upon filing because it effects a change in a due, fee, or
other charge applicable only to a member. At any time within sixty days
of the filing of such rule change, the Commission summarily may
temporarily suspend such rule change if it appears to the Commission
that such action is necessary or appropriate in the public interest,
for the protection of investors, or otherwise in furtherance of the
purposes of the Act.
---------------------------------------------------------------------------

    \8\ 15 U.S.C. 78s(b)(3)(A).
    \9\ 17 CFR 240.19b-4(f)(2).
---------------------------------------------------------------------------

IV. Solicitation of Comments

    Interested persons are invited to submit written data, views and
arguments concerning the foregoing, including whether the proposed rule
change is consistent with the Act. Comments may be submitted by any of
the following methods:

Electronic Comments

     Use the Commission's Internet comment form (http://www.sec.gov/rules/sro.shtml); or
     Send an email to<EMAIL_ADDRESS>Please include
File No. SR-CME-2012-33 on the subject line.

Paper Comments

     Send in triplicate to Elizabeth M. Murphy, Secretary,
Securities and Exchange Commission, 100 F Street NE., Washington, DC
20549-1090.

All submissions should refer to File Number SR-CME-2012-33. This file
number should be included on the subject line if email is used. To help
the Commission process and review your comments more efficiently,
please use only one method. The Commission will post all comments on
the Commission's Internet Web site (http://www.sec.gov/rules/sro.shtml). Copies of the submission, all subsequent amendments, all
written statements with respect to the proposed rule change that are
filed with the Commission, and all written communications relating to
the proposed rule change between the Commission and any person, other
than those that may be withheld from the public in accordance with the
provisions of 5 U.S.C. 552, will be available for Web site viewing and
printing in the Commission's Public Reference Room, 100 F Street NE.,
Washington, DC 20549 on official business days between the hours of
10:00 a.m. and 3:00 p.m. Copies of such filings will also be available
for inspection and copying at the principal office of CME and on CME's
Web site at http://www.cmegroup.com/market-regulation/files/SEC_19B-4_12-33.pdf. All comments received will be posted without change; the
Commission does

not edit personal identifying information from submissions. You should
submit only information that you wish to make available publicly. All
submissions should refer to File Number SR-CME-2012-33 and should be
submitted on or before September 25, 2012.

    For the Commission, by the Division of Trading and Markets,
pursuant to delegated authority.\10\
---------------------------------------------------------------------------

    \10\ 17 CFR 200.30-3(a)(12).
---------------------------------------------------------------------------

Kevin M. O'Neill,
Deputy Secretary.
[FR Doc. 2012-21634 Filed 8-31-12; 8:45 am]
BILLING CODE 8011-01-P


[Federal Register Volume 77, Number 216 (Wednesday, November 7, 2012)]
[Notices]
[Pages 66856-66857]
[FR Doc No: 2012-27249]

=======================================================================
-----------------------------------------------------------------------

DEPARTMENT OF HOMELAND SECURITY

Coast Guard

[Docket No. USCG-2012-0980]

Merchant Marine Personnel Advisory Committee: Intercessional
Meeting AGENCY: Coast Guard, DHS

ACTION: Notice of Federal Advisory Committee Working Group Meeting.

-----------------------------------------------------------------------

SUMMARY: A working group of the Merchant Marine Personnel Advisory
Committee (MERPAC) will meet to work on Task Statement 77 concerning
the development of new performance measures which can be used to assess
mariner competencies in the International Convention on Standards of
Training, Certification and Watchkeeping for Seafarers, specifically
Electro-Technical Officers and Electro-Technical Ratings. This meeting
will be open to the public.

DATES: A MERPAC working group will meet on November 27 and November 28,
2012, from 8 a.m. until 5 p.m. Please note that the meeting may adjourn
early if all business is finished. Written comments to be distributed
to working group members and placed on MERPAC's Web site are due by
November 20, 2012.

ADDRESSES: The working group will meet in Room 5-1224 of Coast Guard
Headquarters, 2100 Second Ave. SW., Washington, DC. Attendees will be
required to provide a picture identification card and pass through a
magnetometer in order to gain admittance to the U.S. Coast Guard
Headquarters Building. Visitors should also arrive at least 30 minutes
in advance of the meeting in case of long lines at the entrance.
    For information on facilities or services for individuals with
disabilities or to request special assistance, contact Mr. Rogers
Henderson at<PHONE_NUMBER> or<EMAIL_ADDRESS>    To facilitate public participation, we are inviting public comment
on the issues to be considered by the work group, which are listed in
the ``Agenda'' section below. Written comments must be identified by
Docket No. USCG-2012-0980 and may be submitted by one of the following
methods:
     Federal eRulemaking Portal: http://www.regulations.gov.
Follow the instructions for submitting comments (preferred method to
avoid delays in processing).
     Fax:<PHONE_NUMBER>.
     Mail: Docket Management Facility (M-30), U.S. Department
of Transportation, West Building Ground Floor, Room W12-140, 1200 New
Jersey Avenue SE., Washington, DC 20590-0001.
     Hand delivery: Same as mail address above, between 9 a.m.
and 5 p.m., Monday through Friday, except Federal Holidays. The
telephone number is<PHONE_NUMBER>.
    Instructions: All submissions received must include the words
``Department of Homeland Security'' and the docket number for this
action. Comments received will be posted without alteration at http://www.regulations.gov, including any personal information provided. You
may review a Privacy Act notice regarding our public dockets in the
January 17, 2008, issue of the Federal Register (73 FR 3316).
    Docket: For access to the docket to read documents or comments
related to this notice, go to http://www.regulations.gov.
    This notice may be viewed in our online docket, USCG-2012-0980, at
http://www.regulations.gov.

FOR FURTHER INFORMATION CONTACT: Mr. Rogers Henderson, Alternate
Designated Federal Officer of MERPAC, telephone<PHONE_NUMBER>. If you
have any questions on viewing or submitting material to the docket,
call Renee V. Wright, Program Manager, Docket Operations, telephone
202-366-9826.

SUPPLEMENTARY INFORMATION: Notice of this meeting is given under the
Federal Advisory Committee Act (FACA), 5 U.S.C. App. (Pub. L. 92-463).
    MERPAC is an advisory committee established under the Secretary's
authority in section 871 of the Homeland Security Act of 2002, Title 6,
United States Code, section 451, and chartered under the provisions of
the FACA. The Committee acts solely in an advisory capacity to the
Secretary of the Department of Homeland Security (DHS) through the
Commandant of the Coast Guard and the Director of Commercial
Regulations and Standards on matters relating to personnel in the U.S.
merchant marine, including but not limited to training, qualifications,
certification, documentation, and fitness standards. The Committee
advises, consults with, and makes recommendations reflecting its
independent judgment to the Secretary.

Agenda

Day 1

    The agenda for the November 27, 2012, working group meeting is as
follows:
    (1) Develop new performance measures which can be used to assess
mariner competencies listed in the International Convention on
Standards of Training, Certification and Watchkeeping for Seafarers
(STCW), 1978 as amended, specifically Electro-Technical Officers and
Electro-Technical Ratings;
    (2) Public comment period;
    (3) Discuss and prepare proposed recommendations for the full
committee to consider with regards to Task Statement 77, concerning the
development of new performance measures which can be used to assess
mariner competencies in the STCW, specifically for Electro-Technical
Officers and Electro-Technical Ratings.

Day 2

    The agenda for the November 28, 2012, working group meeting is as
follows:
    (1) Continue discussion on proposed recommendations;
    (2) Public comment period;
    (3) Discuss and prepare final recommendations for the full
committee to consider with regards to Task Statement 77, concerning the
development of new performance

measures which can be used to assess mariner competencies in the STCW,
specifically for Electro-Technical Officers and Electro-Technical
Ratings; and
    (4) Adjournment of meeting.
    Procedural: A copy of all meeting documentation, including the Task
Statement, is available at https://homeport.uscg.mil by using these key
strokes: Missions; Port and Waterways Safety; Advisory Committees;
MERPAC; and then use the event key. Alternatively, you may contact Mr.
Henderson as noted in the ADDRESSES section above.
    Public oral comment periods will be held during the working group
meeting. Speakers are requested to limit their comments to 3 minutes.
Please note that the public oral comment periods may end before the
prescribed ending time indicated following the last call for comments.
Contact Rogers Henderson as indicated above to register as a speaker.

    Dated: November 2, 2012.
F. J. Sturm,
Acting Director of Commercial Regulations and Standards.
[FR Doc. 2012-27249 Filed 11-6-12; 8:45 am]
BILLING CODE 9110-04-P


[Federal Register Volume 87, Number 45 (Tuesday, March 8, 2022)]
[Notices]
[Pages 12945-12947]
[FR Doc No: 2022-04873]

=======================================================================
-----------------------------------------------------------------------

DEPARTMENT OF ENERGY

Federal Energy Regulatory Commission

Combined Notice of Filings #1

    Take notice that the Commission received the following exempt
wholesale generator filings:

    Docket Numbers: EG22-61-000.
    Applicants: MS Sunflower Project Company, LLC.
    Description: Notice of Self-Certification of Exempt Wholesale
Generator Status of MS Sunflower Project Company, LLC.
    Filed Date: 3/1/22.
    Accession Number: 20220301-5366.
    Comment Date: 5 p.m. ET 3/22/22.

    Take notice that the Commission received the following electric
rate filings:

    Docket Numbers: ER10-1410-005; ER10-1823-003; ER16-1750-008; ER16-
2601-006; ER17-2292-006; ER17-2381-005; ER19-1656-005; ER20-2123-003;
ER20-2768-003.
    Applicants: Greensville County Solar Project, LLC, Hardin Solar
Energy LLC, Wilkinson Solar LLC, Scott-II Solar LLC, Southampton Solar,
LLC, Summit Farms Solar, LLC, Eastern Shore Solar LLC, Dominion Energy
Marketing, Inc., Virginia Electric and Power Company.
    Description: Notice of Change in Status of Virginia Electric and
Power Company, et al.
    Filed Date: 3/1/22.
    Accession Number: 20220301-5265.
    Comment Date: 5 p.m. ET 3/22/22.

    Docket Numbers: ER16-2019-006.
    Applicants: Five Points Solar Park LLC.
    Description: Compliance filing: Five Points Solar Park LLC Change
in Status to be effective 10/1/2021.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5170.
    Comment Date: 5 p.m. ET 3/23/22.

    Docket Numbers: ER20-1996-003; ER21-1187-002; ER21-1188-002; ER21-
1370-003; ER21-1916-001.
    Applicants: Assembly Solar III, LLC, Assembly Solar II, LLC,
Prairie State Solar, LLC, Dressor Plains Solar, LLC, Assembly Solar I,
LLC.
    Description: Notice of Change in Status of Assembly Solar I, LLC,
et al.
    Filed Date: 3/1/22.
    Accession Number: 20220301-5380.
    Comment Date: 5 p.m. ET 3/22/22.

    Docket Numbers: ER21-2445-001.
    Applicants: Glacier Sands Wind Power, LLC.
    Description: Notice of Non-Material Change in Status of Glacier
Sands Wind Power, LLC.
    Filed Date: 3/1/22.
    Accession Number: 20220301-5376.
    Comment Date: 5 p.m. ET 3/22/22.

    Docket Numbers: ER21-2515-002.
    Applicants: Dominion Energy South Carolina, Inc.
    Description: Compliance filing: Order 676-J Compliance Filing to be
effective 12/31/9998.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5061.
    Comment Date: 5 p.m. ET 3/23/22.

    Docket Numbers: ER21-2652-003.
    Applicants: Caddo Wind, LLC.
    Description: Notice of Non-Material Change in Status of Caddo Wind,
LLC.
    Filed Date: 3/1/22.
    Accession Number: 20220301-5257.
    Comment Date: 5 p.m. ET 3/22/22.

    Docket Numbers: ER22-477-001.
    Applicants: Midcontinent Independent System Operator, Inc.
    Description: Tariff Amendment: 2022-03-02_Deficiency Response to
Attachment GGG MHVDC Self-Funding to be effective 2/2/2022.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5194.
    Comment Date: 5 p.m. ET 3/23/22.

    Docket Numbers: ER22-1140-000.
    Applicants: Black Hills Colorado Electric, LLC.
    Description: Sec.  205(d) Rate Filing: Filing of Jurisdictional
Agreements to be effective 12/31/9998.
    Filed Date: 3/1/22.
    Accession Number: 20220301-5262.
    Comment Date: 5 p.m. ET 3/22/22.

    Docket Numbers: ER22-1141-000.
    Applicants: Black Hills Colorado Electric, LLC.
    Description: Sec.  205(d) Rate Filing: Filing of Jurisdictional
Agreements--Wholesale Distribution Servs Rate Scheds to be effective 5/
1/2022.
    Filed Date: 3/1/22.
    Accession Number: 20220301-5274.
    Comment Date: 5 p.m. ET 3/22/22.

    Docket Numbers: ER22-1142-000.
    Applicants: Versant Power.
    Description: Compliance filing: Order No. 676-J Compliance Filing
and Request for Waivers to be effective 12/31/9998.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5040.
    Comment Date: 5 p.m. ET 3/23/22.

    Docket Numbers: ER22-1145-000.
    Applicants: Black Hills Colorado Electric, LLC.
    Description: Sec.  205(d) Rate Filing: Filing of Jurisdictional
Agreements to be effective 12/31/9998.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5062.
    Comment Date: 5 p.m. ET 3/23/22.

    Docket Numbers: ER22-1146-000.
    Applicants: Midcontinent Independent System Operator, Inc.
    Description: Sec.  205(d) Rate Filing: 2022-03-02_SA 1756 METC-
Consumers Energy 15th Rev GIA (G479B) to be effective 2/1/2022.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5070.
    Comment Date: 5 p.m. ET 3/23/22.

    Docket Numbers: ER22-1147-000.
    Applicants: Idaho Power Company.
    Description: Sec.  205(d) Rate Filing: Section 4.2--Sliding Yearly
Update to be effective 5/6/2022.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5104.
    Comment Date: 5 p.m. ET 3/23/22.

    Docket Numbers: ER22-1148-000.
    Applicants: Arizona Public Service Company.
    Description: Compliance filing: OATT Modifications--Pursuant to
Order 676-J to be effective 12/31/9998.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5110.
    Comment Date: 5 p.m. ET 3/23/22.

    Docket Numbers: ER22-1149-000.
    Applicants: Nevada Power Company.
    Description: Compliance filing: Order No. 676-J Compliance to be
effective N/A.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5113.
    Comment Date: 5 p.m. ET 3/23/22.

    Docket Numbers: ER22-1150-000.
    Applicants: ISO New England Inc.
    Description: Compliance filing: ISO-NE; Revisions to Schedule 24 to
Comply with Order No. 676-J to be effective 12/31/9998.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5121.
    Comment Date: 5 p.m. ET 3/23/22.

    Docket Numbers: ER22-1151-000.
    Applicants: Alabama Power Company, Georgia Power Company,
Mississippi Power Company.
    Description: Compliance filing: Alabama Power Company submits
tariff filing per 35: OATT Attachment O Order No. 676-J Cyber and PFV
Compliance Filing to be effective 12/31/9998.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5125.
    Comment Date: 5 p.m. ET 3/23/22.

    Docket Numbers: ER22-1152-000.
    Applicants: American Transmission Systems, Incorporated, PJM
Interconnection, L.L.C.
    Description: Sec.  205(d) Rate Filing: American Transmission
Systems, Incorporated submits tariff filing per 35.13(a)(2)(iii): ATSI
submits one ECSA, SA No. 6298 to be effective 5/2/2022.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5136.
    Comment Date: 5 p.m. ET 3/23/22.

    Docket Numbers: ER22-1153-000.
    Applicants: Mid-Atlantic Interstate Transmission, LLC, PJM
Interconnection, L.L.C.
    Description: Sec.  205(d) Rate Filing: Mid-Atlantic Interstate
Transmission, LLC submits tariff filing per 35.13(a)(2)(iii): MAIT
submits seven ECSAs, SA Nos. 6292-6297 and 6299 to be effective 5/2/
2022.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5138.
    Comment Date: 5 p.m. ET 3/23/22.

    Docket Numbers: ER22-1154-000.
    Applicants: PJM Interconnection, L.L.C.
    Description: Tariff Amendment: Notice of Cancellation of ISA,
Service Agreement No. 5235; Queue No. AB2-068 to be effective 5/22/
2022.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5144.
    Comment Date: 5 p.m. ET 3/23/22.

    Docket Numbers: ER22-1155-000.
    Applicants: Midcontinent Independent System Operator, Inc.
    Description: Compliance filing: 2022-03-02_Attachment LL CMP NAESB
WEQ Standards Compliance to be effective 6/2/2022.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5149.
    Comment Date: 5 p.m. ET 3/23/22.

    Docket Numbers: ER22-1156-000.
    Applicants: Midcontinent Independent System Operator, Inc.
    Description: Compliance filing: 2022-03-02_MISO-PJM JOA CMP NAESB
WEQ Standards Compliance to be effective 6/2/2022.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5155.
    Comment Date: 5 p.m. ET 3/23/22.

    Docket Numbers: ER22-1157-000.
    Applicants: Southwest Power Pool, Inc.
    Description: Compliance filing: Order No. 676-J Compliance Filing
Revising the SPP-MISO JOA to be effective 6/2/2022.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5158.
    Comment Date: 5 p.m. ET 3/23/22.

    Docket Numbers: ER22-1158-000.
    Applicants: Midcontinent Independent System Operator, Inc.
    Description: Compliance filing: 2022-03-02_RS 8 Manitoba Hydro MISO
Agreement NAESB WEQ Standards Compliance to be effective 6/2/2022.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5162.
    Comment Date: 5 p.m. ET 3/23/22.

    Docket Numbers: ER22-1159-000.
    Applicants: Midcontinent Independent System Operator, Inc.
    Description: Compliance filing: 2022-03-02_MISO-SPP JOA CMP NAESB
WEQ Standards Compliance to be effective 6/2/2022.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5164.
    Comment Date: 5 p.m. ET 3/23/22.

    Docket Numbers: ER22-1160-000.
    Applicants: Midcontinent Independent System Operator, Inc.
    Description: Compliance filing: 2022-03-02_RS 46 Minnkota MISO
Agreement NAESB WEQ Standards Compliance to be effective 6/2/2022.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5167.
    Comment Date: 5 p.m. ET 3/23/22.

    Docket Numbers: ER22-1161-000.
    Applicants: ISO New England Inc., Eversource Energy Service Company
(as agent), New England Power Company.
    Description: Compliance filing: ISO New England Inc. submits tariff
filing per 35: Rev. to Schedules 20A and 21 of the ISO-NE Tariff to
Comply with Order 676-J to be effective 12/31/9998.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5174.
    Comment Date: 5 p.m. ET 3/23/22.

    Docket Numbers: ER22-1162-000.
    Applicants: Tucson Electric Power Company.
    Description: Compliance filing: Order 676-J Compliance Filing to be
effective 12/31/9998.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5178.
    Comment Date: 5 p.m. ET 3/23/22.

    Docket Numbers: ER22-1163-000.
    Applicants: PJM Interconnection, L.L.C.
    Description: Compliance filing: Order No. 676-J Compliance
Revisions to JOA between PJM and MISO to be effective 6/2/2022.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5181.
    Comment Date: 5 p.m. ET 3/23/22.

    Docket Numbers: ER22-1164-000.
    Applicants: UNS Electric, Inc.
    Description: Compliance filing: Order 676-J Compliance Filing to be
effective 12/31/9998.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5185.
    Comment Date: 5 p.m. ET 3/23/22.

    Docket Numbers: ER22-1165-000.
    Applicants: PJM Interconnection, L.L.C.
    Description: Compliance filing: Order No. 676-J Compliance
Revisions to Tariff, Section 4.2 to be effective 6/2/2022.
    Filed Date: 3/2/22.
    Accession Number: 20220302-5187.

    Comment Date: 5 p.m. ET 3/23/22.
    The filings are accessible in the Commission's eLibrary system
(https://elibrary.ferc.gov/idmws/search/fercgensearch.asp) by querying
the docket number.
    Any person desiring to intervene or protest in any of the above
proceedings must file in accordance with Rules 211 and 214 of the
Commission's Regulations (18 CFR 385.211 and 385.214) on or before 5:00
p.m. Eastern time on the specified comment date. Protests may be
considered, but intervention is necessary to become a party to the
proceeding.
    eFiling is encouraged. More detailed information relating to filing
requirements, interventions, protests, service, and qualifying
facilities filings

can be found at: http://www.ferc.gov/docs-filing/efiling/filing-req.pdf. For other information, call<PHONE_NUMBER> (toll free). For
TTY, call<PHONE_NUMBER>.

    Dated: March 2, 2022.
Debbie-Anne A. Reese,
Deputy Secretary.
[FR Doc. 2022-04873 Filed 3-7-22; 8:45 am]
BILLING CODE 6717-01-P