The cancer genome, the cancer genome atlas, as the name suggests, has been focusing on genomic, identifying genomic events and did not include a large-scale proteomics profiling. Gordon Miller and Edin Lue from MD Anderson, they just started to measure protein level and possible protein levels in TCG sample. And we kind of get this data and we are playing around with it and we tried to correlate those proteomics data with those genomic alterations. And I should point out that this presentation is more about working in progress and we just started to scratch the surface of this interesting data. So RPPA stands for Reverse Phase Protein Array and it's a high-throughput proteomics technique to measure protein level and the possible protein level in relatively expensive way. Each site is developed with a single antibody and could contain up to 1,000 samples with controls and there are currently about 300 validated antibodies available. The Edin Lue and Gordon Miller from MD Anderson, they have generated those RPPA data so far. In six cancer types for more than 2,000 samples and including like about 200 antibodies, among which 50 antibodies are first for phosphorylation antibody. Here I list a few. For protein antibodies, including like P10, TP53, ER, AR, AKT, et cetera, for phosphorylation antibodies, including AKT, EGFR, B2, MAPK, et cetera. And we should note that several of the genes have both protein level and phosphorylation level. For example, the ERB2, ERB3, AKT, and MPK. And this is a pathway overview of the antibodies. The pink and orange dot indicate that the particular gene have first for antibody, first for antibody and protein antibody respectively. And from this field it's clear that the antibodies actually covers a lot of members in those interesting important signaling pathways in cancer. And one might doubt that if this technique, RPPA, if it is working for TCGA samples. And in the next few slides I will just briefly give some examples that can confirm our expectation from the data. And here is the ERB2, here's the correlation between ERB2, MRA, protein level and phosphorylation level. And from the slides we can see that the ERB2, phosphorylation level is highly correlated with ERB2, MRA expression. And ERB2, phosphorylation level is also correlated with the ERB2 protein level. And we also study, compare the ER and GATA3 protein level in breast cancer subtypes. And as expected the protein level of basolite and ERB2 is significantly lower than the lumina samples. And the same with the GATA3 protein level. And as Giovanni have mentioned, and the PTEN deletion and under expression are highly correlated with ATK phosphorylation. Actually it's across multiple cancers. And so I hope that at this point we have more knowledge about this RPPA. We have some confidence in the RPPA technology and we want to dig into this AKT phosphorylation in breast cancer. So the AKT activation and phosphorylation has been found in many cancer types. And phospho-AKT has diverse targets regulating a lot of important processes in cancer. And phospho-AKT contributes to breast cancer progression and confers resistance to conventional therapies. Inhibiting phospho-AKT could be beneficial to some patients. Okay, this is a box plot and histogram for two important phosphorylation site level in AKT. And clearly we can see that there is a long tail for both of the phosphorylation sites that has a higher phosphorylation level. The question is what genomic even causes AKT activation in breast tumors? From our pathway overview, we have some usual suspects including like PTEN, KRAS, B2, and PGFR1, and PIC3CA. The PTEN deletion and loss of expression is highly associated with phospho-AKT but not PTEN mutation. And for those KRAS, B2, FGFR1, and PIC3CA, actually they are not highly associated with high AKT phosphorylation level. So we wanted to know what other genomics even can explain AKT phosphorylation in breast cancer. To approach that, we separate the samples into AKT high phosphorylation and AKT low phosphorylation, and perform enrichment tests of all gistic region of interest and frequently mutated genes. And we found a couple of interesting carbon number changes, this too, and a few mutations that are significantly altered in AKT high, with samples with higher phosphorylation of AKT. And we actually test all of those genomics events and find that one of the application and AKT E17K mutation may be related to higher AKT phosphorylation level. Again, I should mention, again, this is just a surface. We just scratch the surface of those great data and we think that the data is very interesting and definitely worthwhile digging more. And we found that it's, we have observed very good correlation between gemomics and proteomics data on the level of individual genes and proteins. But the downstream effects of protein level and phosphorylation level is harder to link to genomic events. In the case of the AKT phosphorylation, we can explain some, but not all. Or we're in the progress to systematically investigate those genomic events combinations for all antibodies. And we think that correlations between phosphorylation data may help elucidate, activate signaling pathways. This IPPA data has been integrated in the CBIO GDAC portal and that I would like to thank Nicky, Giovanni, Ethan, Ben and Chris from MSKCC and Gordon Mills, Yiling Lu and the IPPA team from MD Anderson. And thanks for your attention. Thank you.


Ond llawer i fod yn ystafel nhw, rwy'n gweithio sy'n gweithio yma ar y cwmorth. Rwy'n gweithio bod ymddangos yn ymddiol yn ymddiol a'r gwennau'r hoff y ddechrau. Ond oedd yn ardal y cwmorth, rwy'n gweithio'n hoff yn ymddiol. Yn bod ydych chi'n gweld golffu, na'r gwbl yn cael ei gwybwch yn cael ei gweithio ymddangos sy'n cael ei gweithio'n gweithio'n gwybwch o'r amser yn ymwysigol gyda'r mynd yn ymwyaf. Felly, mae'n yn mynd i fod yn ymwysigol. Ac yn ymwysigol, mae'n gwybod dwy o'n rhan o gwybod o'r llwyddoedd mewn gyda'r cyfwysigol. Mae'n llwy ymwyaf, mae'n llwyddoedd o'r llwyddoedd. Mae'n llwy o'r amser o'r amser wedi bod yn cael ei gwybod ymwyaf o'r rhan i'n dwylo. Ond wedi'u gŵl, mae'n gwybod i'r gwybod i'r amser. Y clwb yw'r gael o'r teulu, mae'n gweithio'r gael o'r gael o'r hanes. Mae'n cael graffaith iawn. Mae'n cael graffaith iawn, yn 40 gram o'r gael yn y draf, ac yn 40 gram o'r iawn. Cyn gwych yn ymddiol, mae'n gweithio'n gweithio. Mae'n gweithio. Mae'n gweithio'n gweithio. Mae'n gweithio'n gweithio. Rwy'n gweithio'n gweithio. 140 gram o draf yn y draf, 140 gram o'r iawn. Mae'n cael 8.4 o bobl. Mae'n cael ei ffordd o'r draf yn gyffredig sy'n eich gweithio. Mae'r draf yn 44 o bobl, ac mae'n cael 12.5 o bobl o'r gweithio. Rwy'n gweithio'n gweithio'n gweithio'n gweithio'n gweithio. Felly, roedd. It's horseshoed! I think it's too firm! guess right! there's a clue because I'm using that as a stick and the irons in this set are also three quarters of an inch shorter than my standard all these changes, more loff, shorter length of shaft they're all designed to do one thing and that's give a golfer greater control play more accurate golf and not be chasing distance which can be dangerous! ychydig. That's another super high launching ball. I may be playing from the men's tease right now, but these clubs are in fact tailor made women's. You're probably spotted already, they are the Kalea range. And that's not necessarily the bit that is embarrassing in terms of putting these in the bag. It is perhaps more to do with the weight of shaft and we all recognise this type of thing as perhaps being a senior flex best described. And that's the kind of thing we struggle to accept. Now I know for a fact that in terms of YouTube it's not just me that has found these clubs super easy to play. I've seen James Robinson's feature them at least two, maybe even three times on his channel and I've got to admit going back to them again this morning does beg some big questions you know. I've done things recently about lightweight driver shafts. I think they had a 36g shaft in a Zexio driver fairly recently and was pretty much blown away by it. I find exactly the same every time I pick these things up you know. Golf seems to be a lot damn easier to play with these lighter shafts just as long as you consider that tempo issue. Now one thing you'll also notice with a bag of clubs such as Kalea is there's a lot of head covers and what I mean by that we've got a lot of hybrids we've got a lot of fairway woods and not as many irons and that's because yet again these things get the ball from A to B very effectively right. We're so far green in red, fairways in red let's see if we can keep that going. That's a super strike. I've just got it down the right a tad. It's straight. I'm on a real difficult line and we've got a nasty bounce over to the right hand side as well but in terms of picking that up it was superb that was a four hybrid by the way out of this Kalea range. It's again 40 light flex shaft. It's the same shaft throughout the bag in terms of weight which is also really interesting and they feel exactly the same as a huge amount of consistency whether you've got a wedge in your hand or your driver in your hand. Today's video is brought to you in partnership with HotGolf the online golf mega store bringing you the hottest deals in golf and of course the clubs featured in today's video find the link to the HotGolf website in the description below and check out some incredible giveaways and offers. Now obviously I'm not going to start walking around with a set of ladies clubs in a bag like I said beyond the embarrassment stage that I'm prepared to go but the question for me is I'm going to try an A wedge here by the way we've cut up pin high my greens and regs that has also fell off but the bit for me is this super super light shaft that I keep on playing and the question I've got for you that I ask a lot of occasions is I know what you've got in your driver's shaft currently or your iron shaft because I do ask the question quite frequently but I'm perhaps more interested in have you ever tried any other shafts or is it a case of when you go for a custom fit away by a club you go and I'll tell you what I am a 60 gram stiff driver's shaft I'll order me a new club 60 gram stiff driver's shaft without even contemplating anything else because I would love that type of golfer which would have included me to just go out and try something maybe even right down to the opposite end of the spectrum try a 40 gram driver's shaft out and see what happens anyway we're going to need to make up and down I'm sitting on a T-peg Jesus right a little dink with the A wedge and release to the hole I think we've got it a bit heavy haven't we we've got it a bit heavy and it wasn't a good shot right we're going to have to use our spider putter and see if we can save a par because after the start with a birdie I really fancied making it good fun here what a terrible chip shot fortunately I don't think I can blame that on the light weight of shaft nor the clear irons can this putter save me I was a little bit fiery on the first one see we can take a little bit off off the left hand side and break no didn't break didn't break still fiery and I've now left a horrible one to save bogey yeah well firm and straight we've got more and more holes to play on one over I want to make birdie and it's a par 5 and I reckon we can do it you know now if you missed out on our recent big news video was to tell you that we've launched a new channel it's called testing the tips and I'd love it if you pop on over there and consider subscribing and supporting that channel basically here's me testing tips from fellow golf YouTubers to try and improve my game and hopefully improve yours right shot tracer is on gloves going on we've got a par 5 to finish it's not an overly long one either 480 yards I'm still off those white tees I've still got a ladies driver in hand this has been really good so far can we finish off slow that down a bit get that tempo it's being the main concentration so much better so much better let's get that ball landed for shot tracer first of all that's gone a long way as well the interesting thing is in terms of this head to me it's very much like the sim 2 head as well thing absolutely fires out there 12.5 degrees of loft means it's firing really high as well I love that kind of ball flight a way down there I almost think there's a chance with these firm fairways like possibly get up in 2 because I think we've got a 5 wood or maybe even a 7 wood will get there right we've got 210 yards to go just a bit over that it's about 214 I think but I want to use a 7 wood because I reckon if we can land this probably 20 yards short it's going to make keep that tempo good that's beautiful isn't it let's do that my god the ball flight I can't see that for a minute it's gone that high just back in I'm just hanging on there I cannot believe that ball flight it's gone into the clouds I just could not pick it up at all and it was slightly left of target but it has bounded on we haven't quite made the green but it's certainly right in amongst the fringe there and up and down gets me a birdie which means I'll finish level par with ladies clubs and 40 gram shafts well do you know what my eyesight isn't seeing you that was a good spot down here now then not really got the pace on this putter right so far and I'm determined to make a birdie here this needs hitting up the hill and then off the right okay just roll out a bit more for me that could be really good oh it was getting there it was getting there what we going to do just walk straight up to it straight in the middle do you think I think that's the plan there's not a great deal going on with this one surely so for level par with ladies golf clubs 40 gram shafts come on and get in always dribbled it away I cannot believe I missed it the lesson is take your time and to make sure you get that bit right but anyway the lesson from the video was simple like most of them anyone who takes things too literally and says that I'm telling people to shift into 40 gram shafts well that's not the idea of the video is as always to question what you've currently got and maybe think that it's not quite as embarrassing as you think to shift into something a little bit lighter in terms of both weight of shaft and the flex as well because honestly these are possibly the easiest clubs that I have used this far they're certainly very very forgiving they've got good ball speeds they encourage a good swing tempo and I feel pretty confident that I could play some decent golf round 18 holes with this full set in the bag I just missed this kind of idea right as ever thanks for watching give me that one one over two three that'll have to be I'm afraid see you tomorrow night


The cinematic monsterverse is locked and loaded with all of our favourite kaiju and ready to give us the showdown these characters deserve. Join me today as we discuss the first official trailer and what we can expect from this head-to-head battle of monster proportions. So firstly let's take a look at Kong's introduction to this world already under Godzilla's reign. Now most Kong movies, before Skull Island at least, had Kong taken against his will to civilization where he inevitably breaks free and destroys the city in an act of fury and desperation. This time it appears to be in an effort to defend the city from these kaiju, but just as they say in the trailer, something is provoking them to attack each other, and they even mention an ancient war meaning it just might be their instincts to attack each other. But before we get into it, speaking of epic battles, this video is brought to you by Raid Shadow Legends. Have you ever dreamed of fitting an amazing truly AAA quality game right in your pocket? Something you can dive right into and play at any time? Well now you can with Raid Shadow Legends. Raid's a tough game, it's got 10 challenging dungeons, each with a super tough boss that requires specific teams and strategies to beat. If you care about storytelling, it's got an awesome fully voiced campaign with 12 unique locations. There are 13 unique factions and an incredible 460 champions, each totally unique with their own design skills and strengths. Raid's also super active with 200,000 clans and 25 million players to prove that no matter what you like, Raid's got something for you. Personally my favorite part of Raid is although it's a complex and deep RPG, it's so easy to pick up and play. Select a battle on your favorite champions as you see here, my go to is the archer champions like Sniper, and next thing you know you're right in the middle of the action, ready to grind that XP to go head to head with the next big bad boss. And this month Raid's got their biggest ever update out, the main event is already here, the Doom Tower.  There's really never been a better time to start playing and if you want to get a huge head start in Raid all you have to do is hit the link in the description and you'll get your free void champion, an XP booster, 50 gems, some energy refills and even an ancient shard as soon as you get in game. All this as well as access to the one and only Bulwark. Look at this cool champion you'll get for free, he's gonna be a huge help in the Doom Tower. You'll find your extra rewards here in your inbox for the next 30 days only, you can find me in game under the name Mr. M Rogers. All you need to do is click the link in the description and I'll see you in game. Now back to the video. In true Kong fashion he has a girl that he becomes attached to, this time in the form of a child who Kong feels a loyalty to communicate to and protect, but my guess is this loyalty becomes a big weakness to Kong later on. So far my favourite part of the marketing for this movie aside from the incredible post art that all these monsterverse movies seem to have by the way is the tagline, one will fall. And to that I say thank god, because we all know these showdown movies always end with them teaming up in the end, and I don't think I'm alone in saying that needs to stop. During filming director Adam Wingard said to Entertainment Weekly quote, I do want there to be a winner. The original film was very fun but you feel a little let down that the movie doesn't take a definitive stance. People are still debating now who won in the original movie, so I do want people to walk away from this film feeling like okay there is a winner. End quote. The original film is referring to of course being King Kong vs Godzilla from the 1960s, that movie being a couple of actors fighting in monster costumes. It obviously aged terribly but being from a time before CGI you've got to admire their creativity, and it's definitely got a special place in fans hearts. Thinking this fight through to its conclusion I'm starting to think that as Godzilla is king of the monsters, this movie could see Kong defeat him and become, wait for it, King Kong. But is that what they want us to think? I'm not sure. Personally I'm probably a slightly bigger fan of Godzilla and would love to see more laser blast into the mouth of his opponent as a final finisher, but we've seen that all before and it looks like Kong has some sort of melee weapon to combat his lasers so it really is anyone's fight. Now a potential major spoiler here, you have been warned. Going through this frame by frame, about 4 seconds into the trailer you'd be forgiven for thinking that that's mecha Godzilla overhead. And there has been toy leaks of the mechanical giant so it's definitely not ruled out. Unfortunately Wingard has had a sketchy run with directing movies. Personally I really loved Your Next, a quality home invasion horror but let's not forget he was responsible for the live action Death Note which is borderline unforgivable. But obviously a Kaiju monster movie is a far cry from what he's done previously and I am willing to give him a chance. So let's take a look at the synopsis for the movie. It says, quote, In a time when monsters walk the earth, humanity's fight for its future sets Godzilla and Kong on a collision course that will see the two most powerful forces of nature on the planet collide in a spectacular battle for the ages. As Monarch embarks on a perilous mission into uncharted terrain and unearthed clues to the titans origins, a human conspiracy threatens to wipe the creatures both good and bad from the face of the earth forever, end quote. I don't think I'm alone in saying that you don't watch these movies to see what the humans do but I totally get that it is necessary even if it is just to fill the usual two-hour-ish runtime. But who will be the cast playing out these events? Will we have some of the king of monsters cast returning including star Millie Bobby Brown?  We also have Alexander Skarsgaard joining Rebecca Hall and Brian Tyree Henry. So this movie is set almost 50 years after the events on Skull Island, meaning that's a lot of time for Kong to grow into a faux Godzilla's own size, and they do seem to shape up to each other height-wise in the shots we've seen, towering over the city in the poster. So Godzilla vs Kong is part of Warner Bros and HBO Max's partnership and will be in cinemas and available to stream on the 26th of March 2021, meaning we likely won't be waiting for more than a couple of months. But for those of you that just can't wait, I've been watching a few Godzilla movies that I haven't given a chance until recently, one of which being 2016's Shin Godzilla, a Japanese made Godzilla movie which is basically a soft reboot of the franchise, remaking the original Godzilla movies in modern day, including the politics of how the Japanese government would handle such a situation. Again, it is still more of the standard humanity vs monster plot we've seen our fair share of. But the visuals in this movie are incredible and having the director of Neon Genesis Evangelion, I would expect nothing less. The amount of damage to Japan shown is just incomprehensible when the scale of Godzilla is just terrifying, giving us some really memorable scenes. Another piece of Godzilla content I'm consuming at the moment is the Godzilla anime trilogy on Netflix. I'm currently up to the second one and so far they aren't bad. Not a huge fan of the 3D anime art style personally, but the monsters look badass all the same. Definitely worth your time if you're after a few films to scratch that monster itch in the meantime. But back to the movie at hand, who do you think is going to be the victor, out of Godzilla and Kong? Will Godzilla manage to maintain his well-earned crown, or is it Kong's time to earn the title of King Kong? Let me know or I'll be down there in the comments. But be sure to subscribe for more videos covering your favourite movies and TV shows. If you subscribe during this video then walk them aboard and if you had a good time hanging out then SPANK that like button. This is Matt Rogers and that is all.


Hello and welcome to the Ed Hodl show presented by Buffalo Wildlands. I'm Greg Groy showing this season football coach Ed Hodl Coach you opened up conference play last week You went up to Delaware Valley and hard-fought game on both sides were an early hole where a little battle back But ultimately I didn't get the win just talking a little bit about the game and how it went for you guys Well, I think you know, I was proud of the guys went on the roads hostile environment You know played pretty well You know, I still don't think we've played our best football. Yeah you know, I think we've Finally getting clear of the injury bug a little bit and I think the guys are learning the offense I think it's really we had some opportunities to put the game away late and we weren't able to make plays We really needed to and you know for us, it's still working to be the best football team that we can be Yeah, absolutely. I'll see you had chance to look at the film or some good things You think you did and maybe a couple things that you still need to work on Well, I think one of the most surprising things about the games with the ball for 38 minutes I don't know that I've ever seen the stat line where we had the ball for 38 minutes You know, we were able to control the clock Limited our turnovers. I think we had one turn over. Yeah, we won the turnover battle And I think we played tough physical football. Yeah, which obviously, you know playing a team like that You have to do and I don't know that we had been as physical as we were on Saturday So if we can take that put it together with scheme, I think the rest of you should look pretty good Yeah, now shifting gears this week You head back out on the road go up to FDU floor on the team that you know can always be kind of thorn in your side at times Just talk about again going on the road and you're going for on the road for the third time in four weeks and you know just Another conference opponents look forward to sure, you know go on the road for us. You know, it's a long trip There's always traffic in North Jersey. That's that's the one consistent thing up there But you know for us, it's a again We try not to look at who we're playing right we want to be the best football team that we can be That's a football team that's it's been on the steady rise over the last couple years and You know, they've got some good athletes that do some good things schematically that we're gonna have to be prepared for We're very excited for the challenge. Yeah. All right coach Well wish you the best of luck this Saturday, and we'll chat next week and have some home games look forward to Look forward to it. All right Fred Hodel. I'm Greg Royce. This has been the Ed Hodel show


What's going on everyone? Sam here with late in sports cards, ripping 22-23, pinning 1-1, basketball hobby, 10 box full, case break, number 28. Tiered random team style, there's a look again at your random results. As always, you can find those all on the website under the results tab, as well as the link that's in chat. So, good luck. Super bounty away for now. Here we go. See a timeless moment, or see an awesome downtown. I'm in on either. I'm in on either. Should be, to be honest. Starting off with buddy healed to 99. Congrats on that one. Paces. And then, let's start off hot. Eight of 25, Chet Holmgren. Go into the thunder. Congrats, Brayden. Good stuff, buddy. Eight of 25, Chet Holmgren. I'm going to send an empty box and wrapper, because that isn't on the, that's like in between the mag and the card right there on top there. So, I'm going to send an empty box and wrapper just to be safe, Brayden, but the card itself looks okay. It looks like you can take that off of the card, though. You just, I think you'd have to open the, what's it called? That's the only thing.  Very nice, Chet. All right, starting off with a Clay Thompson, two 15 on the red. There you go, warriors. And then, A, Walker Kessler, 61 and 99, Rookie Otto for the Jazz. Congrats on that, Utah. Anthony Melton, two 99 for the Sixers. Is going to be an RPA of Shadon Sharp, 40 of 49 for Portland. Congrats, Brayden K, again. Good stuff, buddy. Three good rookies. Three colored, yeah. Very good. RPA on the Shadon Sharp. Probably all three rookie first teams, I would imagine. Nice. That is going to be a Chet Holmgren, 20 to 25 on the rookie. Purple rookie, very nice. And then for the Rockets, Rookie Patch Auto, Ty Ty Washington, two at 35. Congrats on that one. Very nice. Rockets. Two 99, Human Highlight Reel for the Hawks. That's the Kings it looks like. And Jalen Green, 10 to 25. Second year, Jersey Auto that's game used as well. 10 of 25. Remember that Kings players? They got that logo right over his face. Yes. Sorry, buddy. Wrong place, wrong time on that one. Next up is a Jalen Green, 225 for the Rockets again. That is going to be an RPA, four of five, Dyson Daniels. Congrats on that one there. Pelicans. That's nasty. Let me tell you right now, Milan. There are currently one, two, three orders in queue. No. One, two, one, two, three, four orders in queue. Four orders in queue. Tiger's super fan. Is that Spencer? I don't know. Must be the guy who has carried Bond spot in all of our stadium club. One of 25, John and Kaminga. All of our pick your players. Congrats on that to Warriors. And timeless moments. Come on. It is Manu Genovely, 17 to 75 for the Spurs. Brayden Kay, congrats again Brayden.  That should be the one. Just smacking it. Game two of the 2012 Western Conference Finals. Fire. Lefty legend. Manu. We've got Jabari Smith, rookie to 40 on the blue. Grads on that. Rockets again. And then a Nicolay Jovic, 32 of 49, three colored RPA going out to the heat. Grads my A. I was going to be a Mark Williams, rookie to 99 for the Hornets. And then nice. Five of five, Cade Cunningham. Second year patch auto game use. That's kind of nasty. Patrick Y, congrats buddy. Hell yeah man. Five of five, Cade Cunningham. That's gross. Free Cade. It's a 99 Brandon Ingram. Nice one there. And then A, another Dyson Daniels RPA, 34-35. Congrats to New Orleans. That's Bob H on the tier two. Not bad. Grads on that. Pelicans. Let's go. Three cap here everybody. We've got A, Dyson Daniels. Walker Kessler. Manu Ginobili to 75. 249 Shade on the shark. Nicolay Jovic and a 35 tie tie Washington. Thank you. Thank you Washington. We've got 225 Chet Holmgren. Jalen Green, game used. It's a five Cade Cunningham game use patch. And a five A at Dyson Daniels RPA. That will do it for the break everybody. Thanks again for the film. Really do appreciate that. Ten box Tuesday coming up now.