So today we're going to talk about the nine classy behaviors Men always notice and find wildly attractive in women or in a woman and let me just say this These are those men who are emotionally grown up. So let me be clear. This isn't for those dysfunctional type of men This isn't for those clinical Men who have clinical issues. This is for those emotionally grown up men now before we get into this I think it's really important to address the elephant in the room and that is Dating in our current environment can keep a person Disillusion frustrated or worse giving up hope and I was actually giving up hope of finding a suitable partner that believing that a Suitable partner exists. So I'm gonna read to you a blog I wrote that will illustrate this and then we're gonna dive into these Excuse me classy behaviors. I Said dating in our current environment can leave a person disillusioned frustrated or worse giving up hope that a suitable partner exists The minute we connect with another human being we make ourselves vulnerable to misalignment Which is just really a nice way of saying we're not a fit for each other After many encounters that go nowhere One can look back and say there's no one for me Now one can listen to those rah-rah coaches that will tell you that you know, all you have to do is love yourself And let me be honest here. I've said that myself But the reality is loving oneself is is rather hard to do The fact is dating is very vulnerable Let me reframe that dating is a very vulnerable act and Given we are swimming in a sea of Dysfunctionality Excuse me in the dating marketplace. It's no wonder many prefer choosing not to engage in the process Genuinely connecting with another human being at a heart-centered level is no small feat and Anyone who suggests finding a life mate is effortless has some swap land to sell you as well Because it takes a lot of courage To put oneself out there and more importantly loving oneself through all the disappointments loving oneself through all the disappointments So this is was on my Instagram and my Facebook post today, and it goes with the following mean Dating today is like the Hunger Games. It's challenging brutal and a fight to the death The death of our dreams and our hopes Preparing oneself for through personal development self-help and spiritual work can put the odds forever in your favor by working on yourself Just saying that's my meme I posted today So Loving oneself self-love, you know, there's an interesting book Look at this cover Love yourself like your life depends on it. This is only 60. This is 59 pages and it's all double-spaced. Look at that a lot of white space. Okay Love yourself like your life depend upon it. I cannot even figure out how to pronounce this person's name Why does this relate to classy behaviors? Ladies, I've observed most women abandon Their sovereignty and give their power away to men they give their power away to men And let me tell you something to that wounded man to that man who's got clinical issues that sociopath that narcissist They are highly attracted to women who don't actually feel good about themselves This is why I'm such a big proponent of personal development self-help and spiritual work not because this work Is going to attract a life made in you What it's going to do is most importantly Hopefully own your sovereignty because as I said before women oftentimes give their power away to men And I think this is almost conditioning way back to cave people days 200,000 years ago where women were more dependent upon men for their survival and today most women have are fully capable of Financially taking care of themselves at least here in the United States for the most part that you are not dependent upon men for your sovereignty But Jonathan men are supposed to be the provider protectors They're supposed to do all that work and all I'm supposed to do is be the nurturer and submit to him Folks you can adopt that you can adopt that traditional role that traditional model You have a one and fifth you have a one out of two chance that that relationship is going to be end That relationship will end and you are on your own and if you're in your in your second or third marriage You have a sixty five to seventy five percent chance of it ending. So let me be clear Dependency on anyone or anything Will directly affect your emotional well-being so coming back to this post I'm sharing about dating it is very it is It is challenging to navigate the emotions of Engaging with another human being being even being vulnerable as if just even writing an email to someone Or a text message to someone can be a very vulnerable act and I will tell you there are coaches out there that will trivialize this There are those motivational and empowerment coaches that can tell you to you can push through through anything through willpower You know a simple text message can be an incredibly vulnerable thing And the fact is as many people this pushing through it through willpower is actually emotionally devastating I Can't tell you how many times even a miscommunication on a text message with someone caused me to recoil To go the other direction to not want to communicate with them Because the reality is is we weren't meant to meet through these environments We were meant to meet face-to-face and get a full flavor of a person And yet this is the medium that we're we are so accustomed to and it isn't going to change You know, I wish I could tell you just go do what you love just go do what you love go do things like painting Go do an art class go to a meet-up group and you'll meet your soulmate there Look it I get this happens For people it does It's like a broken clock. It happens twice a day, but the reality is is it's rarely happening organically The only way you're going to shift that organically is if you are in an environment where everybody is single And they're interacting with one another. In fact, I'll be hosting a live event at the end of March And I'm going to start curating singles events So people with men and women alike 50 50 men 50 women at least there's a better chance when you're in a pool of people Instead of just randomly Hoping you meet someone at the grocery store and you're probably going what the heck does this have to do with this topic? It's because I think we have to recognize that Are it isn't about finding the right guy? If you're a woman and it's not about finding the right, you know gal if you're a man This is about finding the relationship within yourself. So these nine classy behaviors aren't going to be simple things like You know brush your teeth or wear nice clothes You know, we're going to go deeper into the emotional attributes that are actually attractive to that Emotionally grown-up man. Okay, and I'm hoping raise your hand say John or hit that like button If you say Jonathan, I want an emotionally grown-up man or write that in the chat box. Okay Okay, so these are the behaviors that are classy for both men and women alike that both Emotionally grown-up men and emotionally grown-up women appreciate in The gender that they're going after. Okay, so the first one This is simple actions matching words You know in our current dating environment, it saddens me how trivial we treat people How dismissive we treat people particularly when it comes to our actions and our words Whether you're a man or woman, this is this is commensurate no matter what gender is Out in the dating marketplace We see human beings who say one thing and they do another because the truth is is we trivialized people I Say we I'm sure you don't think you do but I'm sure there have been times. I know I've done it I've been dismissive I've trivialized because when they're a total stranger you have nothing invested in them See our actions matching words has more value when we have something to gain or something to lose and I'm here to invite everyone to Operate from a place of integrity or at least do your best to operate integrity and I get as I started this broadcast by saying look We can feel disillusion. We can feel frustrated. It can be brutal. It can be the hunger games out there All I'm offering you is putting the odds more in your favor so actions consistently matching words I'm sure you'd agree with that number two be generous and kind you know Believe it or not, I'm gonna tell you as a man I've gone on what feels like thousands of dates and that's not the actual number But it just feels that way. Can anyone relate to that? It feels like you've met a lot of different people. I Can tell you there have been a lot of times where women have been rather selfish and It doesn't sit well with me and I'm sure you've experienced men who haven't who have been selfish Being generous and kind See I think the reason why early dating we are not seeing this is because everybody's defensive. They've got their walls up They're trying to protect themselves and what's happening is the minute you have a wall up you have lost your capacity Okay, you have a propensity. Let me put it that way to be less generous and kind because there's a wall up I'm here to offer that you operate from a place of generous and kindness with no walls up in the early stage of dating and if you have a wall up then go tear that wall down and then revisit dating when that Tear down that wall mr. Gorbachev Hey, has anyone seen the movie atomic blonde with Charlize Theron? It is of anyways, it's about the the fall of the Berlin Berlin wall But that's what we need to do We have to drop our walls because without it We won't as much as we think we're generous and kind the wall is blocking What's innately already inside of most of you so? generous and kind number three They communicate clearly without being right You are Ladies and men they could they communicate clearly without being right, you know, we we are rather Selfish and righteous here in the United States. I can't speak for other parts of the world But there's this almost belief that we're almost at a war with with the opposite sex. I'm right and you're wrong. I See this habitually Ladies I am going to I'm gonna suggest to you there are always two sides to a story Particularly in a relationship and believe me or believe it or not the truth lies somewhere in the middle So learning to communicate clearly without being right now, I'm not suggesting I'm right here I'm just offering perspective you make up the choice Okay But the same time I think a classy behavior that quality or high a grown emotionally grown-up men notice and Appreciate is a woman that can communicate clearly without being right number four They don't use people They are clear about commitment. I think this is where I I've seen this with a lot of men and women as well They are not clear about right up front the standard. They're looking for And I know a lot of women that use men for a free meal You know that let me just tell you something ladies. This is not cool. This is not cool I Somewhere I read in the younger generation 30% of women who are actively dating are just doing it for a free meal That's using someone You know, that's a perfect example of using someone and even if you're not I Actually spoke to another woman. This is another example She says I'm not ready to be in a relationship, but I'm just going out to have a good time Usually and she says the men always pay and I'm like wait a minute You clearly know you're not ready for a relationship You pretend to be ready for a relationship, but you clearly know it but you always but in the backing mind You go well, I can always change my mind, but if you go in with that kind of intent you will use people And I'm gonna tell you that's a classless act and I've seen so many women do this and I see men do this I remember early after my divorce I was a train wreck, but I thought I was ready for a relationship and then the times I knew I wasn't ready for a Relationship I wasn't dating in fact quite frankly many of you know, I have a significant relationship that ended seven months ago I'm not gone on one day. I'm still processing. I'm still grieving I've got myself my my toes in the water And I hold space that I will connect with someone But I I really have only communicated with one person and it's only because they recognize me professionally anyway, okay number five They have their act together Emotionally physically spiritually they have their act together particularly When I say emotionally and physically they don't need drugs or alcohol to get through the day You know, there's a lot of people that use drugs and alcohol throughout the day just to get by through the day because emotionally and they actually dating is a It's kind of a self-medication for people Just that brief encounter that brief interaction with another human being Causes many people to put themselves out in the dating marketplace. Well, let me tell you something One of the most classy behaviors and things men find very attractive is a woman who has her act together Emotionally physically spiritually in her life. Okay. She's not in constant chaos By the way, ladies, you can't stand men who are in constant chaos There is nothing attractive about a person that's going through a lot of emotional up-evil in their life or physical up-evil for example a divorce a divorce is an incredibly traumatic experience dealing with a with a contentious ex-spouse You know if that's what you're dealing with it's going to make it very problem by the way Guys are not attracted to women who have contentious ex-spouses you ladies will be well Let me reframe that emotionally grown-up men And emotionally grown-up women are not attracted to somebody who has a contentious relationship with an ex-spouse An emotionally dysfunctional person will look past that and go well, you know, I see the potential So let me hang my hat on this person But you know that phrase, you know, you're buying, you know, you're buying the cow You're buying you're not just buying the cow. You're buying the farm. You're buying the manure. You're buying everything else associated with it If you're choosing somebody who doesn't have their act together number seven number seven They are introspective and work on themselves to grow beyond their limitation wounds and traumas. I'm repeat that they are introspective and work on themselves To grow beyond their limitations wounds and traumas folks if you follow my channel, you know I'm a big proponent a personal development self-hubbing spiritual work It's why I wrote a book called what the heck a self-love anyway And I continually promote book after book one particular book It's called the Hoffman process the Hoffman process This is a deep dive into healing childhood wounds and adult traumas an introspective person is aware That they they are aware that they can be triggered they can be get defensive they can judge and they look inward and Always go and ask and say to themselves. I'm the common denominator here and So an introspective person wants to grow past their negative patterns their limiting beliefs in their life to grow past their Limitations their wounds and traumas and by the way a trauma or wound doesn't have to be like an abusive parents or Physically abusive or emotional abuse if you could have had a minor trauma that could have ripple effects in your life And so something that's a really classy behavior is that person that's working on themselves whether it's a man or woman, okay, I Think this is a classy behavior a woman who is a nurturer But protective and empathetic a nurturer protective and empathetic now For the man for the woman who seeks the man. He's a protector.  It's the capacity to do both but being empathetic Isn't just about the capacity to feel someone's feelings It's the capacity to genuinely care about someone's feelings. So being protective nurturing and empathetic and last but not least number nine They believe demonstrating trust is paramount in their life and trust isn't just in relationship about fidelity trust is I Have this other person's best interest at hand There is nothing classier than actually having someone else's best interest without compromising yourself You do not this is not about compromising yourself having someone's best interest, but I'm gonna tell you we build trust through through caring about another person's best interest and These are the nine classy behaviors men and women Notice and find wildly attractive and I'm talking about that emotional grown-up person and not that person who's dysfunctional or have clinical issues folks, I recognize we are in a Difficult dating environment if you need some support you see this link right here to schedule a discovery call with me There's links below. I Invite you to schedule a call with me to see if working with a coach is right for you my whole area of expertise is to talk about discernment which will amplify your Intuition so you can recognize those people who's on the same page with you much sooner rather later And as I said earlier, it's the hunger games out there and my job is to forever put the odds in your favor But a bump bump Hey, if you found value in this conversation post a comment below I'd like to hear your thoughts as always if you if you cared for this video Please hit this like hit the like button. Please share this video Please subscribe to my channel and hit that notification bell And if you want to connect with me directly check out those links below the schedule a discovery call with me to find My group called midlife love mastery To find me on Instagram to get the books. I recommend as well All right, it's time for Q&A if you have a question Write the word question and then post the question there after or you can purchase a super sticker super chat There's a little dollar sign in the chat box all the monies from the super sticker super chat goes to a scholarship fund in the name of My son Conor Asley. That's a picture of him right there He's my son who passed away over five and a half years ago in his honor We donate to causes like the Hoffman process Insight Institute and also Scholarships to private coaching as well. So hit that little dollar sign our goal tonight is $50. We would appreciate some love tonight Also, if you'd like to speak to me live, I'm putting a link to the to To so you can join the hot seat if you'd like to talk to me live So again, if you have a question post the question there after so I want to share with everyone Something I posted in my private group called midlife love mastery Bear with me a second while I pull this up and again, there's a link below to join my group So hopefully I can find this Okay, so Bear with me one second It's good, but this was a very vulnerable post that I had about my feelings about Our current dating environment and I hope you can appreciate this Let me just find it's just gonna take a second it was a rather personal share Oh, I hopefully I can find okay in full transparency My heart is just not into dating Dating is not only a vetting process, but also a search process This means you have to put yourself out there to be found and therein lies the rub Connecting with various people Engaging with them via text and within minutes. You can feel I feel a misalignment or worse a disconnect In those moments, I want to abandon all communication which feels rude These starts and stops starts and stops starts and stops are exhausting which causes me to recoil and In the in the pullback I feel like giving up and yet throwing in the towel goes against my true desires People ask me all the time. Is there a better way? Sure, you can go out in public and do what you love and just like the broken clock It rarely works and you can do the law of attraction, but look if you work from home like me It's you're hoping that someone knocks on your front door Anyways, this was a vent and why I'm sharing this with everyone is I suspect you feel the same way I don't think we were meant to do all of these Starts and stops starts and stops starts and stops This is why we have to become better detectives early on first by operating in those nine classy Behaviors I talked about is going to better prepare you when you genuinely love yourself You won't feel as look it. I'm just venting for a moment I'm back on the saddle again, but I'm just venting for a moment Because I know everyone can relate to this. There's nothing easy about Finding a life partner that's really an emotional grown-up Especially when we're swimming in a sea of Dysfunctionality, so I'm just merely here to say I get it I mean I get it and I wish I could blow smoke up your ass and sell you some swap land, but I'm not going to do that Okay, so if you have some questions for me post some questions or join me live Oh, by the way, I want to thank Beach Lover for the $10 super sticker Thank you so much and I want to give props to Margaret for the $8 super sticker and roller girl for the $2 super sticker That means we're $20 and poultry flowers just gave us $5. That means we're only $25 away from our goal And we have a question from Gigi In your experience, where do you see online dating going Wall Street experts thinks the apps are losing subscribers? You know there sir, I think bumble just laid off I think I think out of 1400 employees. I could be wrong on the numbers here. They just laid off 300 employees That's bumble. I don't think dating apps are going away They still represent the number one place people are meeting. I Think what would be better if people just put together better profiles?  Do I see a really good quality profile? That's complete concise well done It's like if I work for a company I had to review resume after resume after resume after resume after resume and I just saw nothing But people that just you know, it's like they went to the job interview with in their pajamas I Think I don't think there's gonna be something magical that's gonna revert us back to Organic way of meeting either. I think you have to listen Finding a life mate is gonna require some Herculean effort First to be emotionally grown up to be in a relationship and then putting yourself out there to be seen I'm here to say you have to be willing You have to be willing to do a lot of work if you're not willing to and we are a spoon-fed here in the United States of Self-serve easy McDonald's type of environments self-serve kind of environment So I think the human beings who do genuinely make heroic effort will get to have better results That's just my opinion Gigi. So thank you Bump bump bump bump bump bump Let's keep going Power of chi just wrote question in your opinion. What does toxic positivity mean? Where you have experienced it recently? Hey Google What does toxic pass a positivity mean? Just want to hear what you Google So I think people who don't honor your pain and Try to gaslight it or spiritually bypass someone's pain could be perceived as toxic Positivity, I think that is quite possible and happens I think it's important to acknowledge people's pain and not to Again spiritually bypass someone with some rhetoric that you know, you can just magical thinking is going to change everything Have I experienced it yeah, but I mean not enough to make a big deal like I don't need to put a fucking label on it Okay, so some people are just you know Some people are trying to help and they don't do it in the best way, you know I don't like the way we label things toxic. Okay, it might be uncomfortable It might be annoying and that sort of thing and you by the way and it's and you can speak up and say look I don't want to hear it anymore. That's you can stand in your power, too so But that's just my 10 cents on that one power of cheese So by the way folks if you want to ask me questions like this I'm happy to do things outside of the dating realm but yeah Nw says, how do you deal with the text from no contact? I want him regret How do you deal with a text from no contact? I want him to regret and W I don't understand your question. So thank you for trying, but I just don't understand the question Barbara says I think apps should do away with algorithms and simply spray everyone that paid and show them all up Rather than filter through 100,000 resumes than just a handful of algorithms You know one way you can bypass that is do a search Okay, you do a search bite bite one year in other words You you pick an age like 49 and you do a five mile search and you'll probably find 20 people that fit that category and then you go to 50 and you go through 20 people that fit that category I think you can actually bypass the algorithms by being really Succinct, I believe I'm assuming that will work But I don't know if the algorithms are intentionally avoiding people You know, I don't know that but I think do searches by hey Just do at age 49 50 51 52 do a five mile radius and then or Expand it to a hundred miles and they're like that's that's just some ideas I have to work around some of the algorithms But I get it Barbara you're a recruiter so you think along the same lines as I do so I get it so thank you Jane Spitfire says I miss the classy behaviors. You're just gonna have to go back and watch I started about the seven minute mark if you want to follow back then, okay? Power of Jesus. Thank you for your opinion. Oh, here's the seven or the nine It's or here's five of them anyway Actions matching words be generous and kind communicate clearly without being right. Don't use people have their act together Healed from past relationships. Oh, I forgot to see I said healed from past relationships They're introspective and work on themselves. They are protective and they believe demonstrating trust. You know what folks I actually missed one of these They have healed from past relationships. That was number six six seven six I forgot to include that. I am so sorry in the original nine. Okay, so they have healed from past Relationships I kind of touched upon that with the With the person that has a contentious X But I forgot to include that so I know in my previous one. I did eight that was number six on the list So, thank you Sunshine Labardi says did you ever think about having your own dating site? Sure I think about it all the time if someone wants to hand me one million dollars to make it happen I will gladly create a dating site if someone wants to hand me that money and they can have all the half of the proceeds Mary Ann says question. I'm curious about your upcoming event Is it going to be a seminar a dinner a dance? Tell me about yes So I am hosting a live event. It's a singles mixer. It's going to be at a restaurant They have a section in the restaurant that can fit about 50 60 people It's going to be open to both men and women I will do about an hour and a half of Conversation starting at 7 p.m. At night That's going to take us till about 8 30 and then it's going to be a mixer where people can interact with one another my goal is to do this once a month in Los Angeles as I Mean the pro I don't get to keep any of the money because it's going to the person who's putting it on for me I'm doing it because I want people to start connect kind of like Like speed dating in a way, but it's not speed dating because you get to interact with people whom you wish to Okay, so thank you Mary Ann. I appreciate that Amy. Thank you for the $5 super sticker That means we only have 20 more dollars to go for tonight. They hit our $50 goal Julie angle is in the house Jonathan this may be random, but why are women stand-offish with me and men have no problem talking to me, especially in the workplace? okay, I vague I I remember I Didn't study this, but I I posed I I posed a question in my Facebook some years ago I did a poll and this seems to be very this seems to be an interesting Dynamic between women and women I Believe women probably on some Subconscious or instinctual level believe that they're in competition with each other For resources, so they're very competitive in the work environment and since men aren't competing with them in the same way Men can be very generous to the women in the workplace and vice versa I believe there's some underlying instinctual or subconscious Competition going on between women women you ladies can be fucking brutal to one another I mean the cattiness I've witnessed over the year God. I've watched movies where this happens So I mean that's just my understanding I am not a behavioral scientist or an expert to give you the real concise reason, but that's just my rough thoughts on that So thank you, Julie for that question Okay, and MJ just pointed out I forgot that at six seven and eight So I forgot to include nine. So again, I forgot to include they healed from their past relationship. So thank you Oh, there you go. You added it right there. So there you go.   Oh that red flag red. No, that was deal breaker deal breaker deal breaker deal breaker I get it whether it's a man or woman someone who's hung up on their past Clearly isn't ready But they think they are because they want to see the problem is we humans, you know Like there's a hole inside of us when our relationship ends and we want to fill that hole immediately We've got to do our best to fill our that hole with our own self-love Mel Stiller says my personal experience has taught me something very important never jump from the flames into the fire Amen to that. Amen to that Power cheese says oops here we go power cheese says It's very true Jonathan women compete with other women in the workplace. Yeah, why ladies? Why is that? Shouldn't be in competition. You should be on the same side Mel says Women are extremely competitive. It could be very nasty to each other. It's kind of ironic. I mean Isn't it isn't it kind of sad and why I say humans are rather dysfunctional That's a perfect example Sally Question wait, so you want someone to hand you all the money to start up a dating site And they only get half the profit wouldn't you be working as the employee and manager and get 50%? By the way, I just was off the cuff ripping. So yeah But if someone wants my expertise, I don't know And that's why I'm not gonna do it because I don't expect it Hey, does anyone want to join the hot seat tonight join the hot seat? It'd be fun to have someone on live Gigi said brutal a neighbor bought 14 of her friends to my house because her husband stared at Me too long at a party. The beaches are over 65. I don't understand that. Okay Men respect each other more brotherhood You know men can be by the way men can be ridiculously Competitive as well. I just think in the workforce They're not nasty about it, but men can be ridiculously competent competitive as well. I Mean, you know, it's one of the reasons why well anyway I'm you know, it's just it's just a fact of life You know, there is a component of human basic need for Survival and within that oftentimes people are very myopic To their actions towards other people. That's just my rough perception on that. Okay Light how or excuse me Sherry says lighthouse men compete against each other as well.  I don't get that one. But thank you Okay, Barbara says I'm a woman and things are actually changing both professionally and in the working It was because we had limited resources. We're slowly gaining more resources from From even men and competition is balancing out. Okay, that's good to hear Julie says men are territorial very true statement. We men can be very territorial Sherry says I see men bullying and competing at work. Yeah, that happens that happens Lighthouse seems to think that men protect each other more than women, you know, I'm I'm grateful I'm not in the day-to-day work environment because I got it Okay, I had a colleague of mine. We went out to line. I had a I had a referral for The the to do business with these two people and I brought one of my Contemporaries and we would all be able to work with these two people but I went to the bathroom And I was gone maybe five minutes, let's say and when I came back He and I we wrapped up the lunch and we came back to the office And I got a text message from or a call from one of the women. She said hey Jonathan I want to let you know what happened your colleague Through you under the bus. He was critical of you. He complained about you. He judged you and And I'm like and she goes because you and I are friends. I'm letting you know what he did And I'm thinking to myself.  So I don't think it had anything to do with that Julie says okay Jonathan, what if a random guy that?  It's just just Not a classy thing to do although I've been criticized that my profanity isn't a classy thing But I'm here to say that Explosives are used to enhance the sentence and I recognize for some of you that my profanity or cursing is offensive to you I get that and you know it and I recognize that I can't please everyone. I can't please everyone I Just use it for a fact Okay, I use it for a fact and if it bothers you I understand if you don't want to watch my videos Okay, and I do it for a little bit do it for the humor as well So and as Sharon says f them all, okay? Thank you Ariel says what do you think of making a dating app with only video profiles? hmm Okay, I've got something personal to share with you guys ladies, okay my Ex-wife and I met through a video dating service now. Let me give you a little bit of the backstory. Okay, so this was 1990 was January of 1990 and Back then there used to be a company called great expectations that You would go into their office. You pay thousands of dollars. You would record a video It would be put into a library There would be like a little profile of picture of you with a little essay and then a person would come in Oh, they would match you up with you know You would review the profiles and pick people you want to meet and you'd watch a video of theirs Sure enough my now ex-wife Aaron had we this was a new company that opened up in Gardena, which is the city next to Redondo Beach okay, and and they put out all these flyers all over Redondo Beach video dating service This is 1990. Okay, and So I looked through a few of the profiles I put in my request for someone what turns out she saw my profile and put a record Oh, no, I saw her profile and put a request. I'd like to meet her So then she had to physically drive to the location View the profile watch the video and then agree whether or not she'd meet me and it turns out I recognized her See it used to be she She used to be she worked as a cocktail waitress at Cheesecake Factory now She was also at the same time. She was putting herself through college She had a job as an accountant and this was her side hustle to make some extra money And I knew her because the bartender there was a old high school friend of mine So anyway when I saw her profile, I said hey, I recognized you and she remembered who I was and So we had a date. We went to El Toritos on the pier I can actually see it right there. All the blinds are down right now, but El Toritos like right there I think it was January 20th 1990 somewhere around that time And we had a first date that turned into a second date that turned into a third date that turned into a fourth date We eventually started hanging out with my friends, you know, it was interesting I don't think we slept together for quite some time by the way dating was so different back in the 1990s It wasn't about getting laid. It was actually about getting to know someone. It was vastly different than today Because there used to be you know, you had to jump through more hoops Visit to get into to sleep with someone you had to be in relationship with someone to sleep with someone Except for one night stands you meet someone at a bar.  Okay Anyway, that is how I met My now ex-wife we met through a video dating service Back in 1990. I was up mid late late 20s back then Anyway, I hope you appreciate that little story Beach lover says oh great expectations. That's how I met my husband 35 years ago way to go Okay, Amy is in the house Five months relationship transition to friends for me But not for him do not want do not want to block him, but he won't accept Don't know what to do. I need to move on to better match you know This is the tough thing when you still have feelings for someone and maybe you even wish to get back together with them I think that's what you're saying is You're blocking him I don't know what to do. I need to move on Wait five months who transits friends for me and not for him So if he doesn't want to be friends, it's because he I'm sorry Maybe he's dealing with the pain and he needs to have you know Space from any friendship because being friends with someone requires sometimes being rather being you see It's one thing to be a social friend with someone It's another thing to be an intimate friend and when you've been physically intimate with someone It's very difficult to transition that because a lot of times there's an emotional Dependency going on so he's sounds like he's created a clear wall that he just doesn't want to go there And I guess your job is to accept that Grieve it and then move on accept it grieve it move on Mel Stiller says eons ago in the age of dinosaurs I met my husband number one at a family barbecue many moons later. I met number two I was out with friends and we went to a bar guess which marriage lasted longer. I Don't know which marriage lasts longer. Please. Let us know met Mel Power cheese says how do you how do you? Income decompress and recharge your social dating battery after dealing with so many dates with draining women Well First off, I haven't the the the speed dating I used to do or serial dating was years ago I mean today I am very selective on whom I'm whom I will communicate with and My hope is that I'm that being selective That so I'm actually interacting with so few people in fact as I said before I haven't gone on a date with anyone Because no one has sparked my fancy enough to want to go on a date with them Again, most dating profiles are so poorly done that I'm I'm rather not interested in many of them So how do you recharge your batteries? You know, I've hold a vision It's like the law of it's not the law of attraction. It's the law of assumption Assumed this person is in my life. I Just already assumed it. I'm just holding space for when she enters in the front door not literally figuratively So I just hold space that that it's I already make the assumption that that person is in my life Okay, that's how I I recharge my battery Sally says do you have do you have dances in America in UK growing up? We used to meet and go dancing at clubs now at a certain age places to do that aren't there Aren't any life was better in the 80s. I love the 80s going out to bars and go dancing. Yes, you can still go dancing But they're not the same as they used to be There aren't as many bars where you go dancing at least locally here where That was called a nightclub which had dancing associated with it. So yeah But they do exist. Okay, they do exist Julie says that's true. Jonathan now everyone wants to get laid. I get it. I get it. I get it. Oh Barbara says bumble and hinge. I forgot to mention that do allow you to put videos up on your site I don't believe match.com allows that but I do I know for certain bumble and hinge For certain allow that okay Disco tax. Yes, Amy says I love the 80s Oh Mel gave us the answer marriage number one lasted longer have two sons in ten years He decided to walk out and play footsie with another woman. So Well Does lasting longer mean anything? I don't know Does it matter if someone was married 30 years or three years that big like they still ended? Anyway, I'm gonna repeat those nine classy behaviors because I screwed up in the original one Okay, and we're gonna wrap up for tonight number one their actions match their words consistently number two They are generous and kind number three. They communicate clearly without wanting to be right Number four, they don't use people. They are clear about the type of commitment. They seek number five They have their act together number six. They have healed from past relationships number seven They are introspective and work on themselves to grow beyond their limitations wounds and traumas number eight They are protective nurturing and empathetic and number nine They believe trust is paramount in their life and those are the classy behaviors That men and women will notice and find incredibly attractive if you're an emotionally grown-up man and emotionally grown-up woman Is this sinking in is this resonating? Please let me know if it is post a comment below I'd like to hear your thoughts on all this as always if you found value in this video Please hit that like button. Please share this video. Please subscribe to my channel hit that notification bell So you can be notified of new videos Also, if you want to connect with me schedule a discovery call. There's links below to Schedule a call with me to join my group called midlife love mastery to find me on Instagram to get all the books I recommend and my dating vows as well. We're gonna wrap up this video as I always do first off Give myself a big gigantic Jonathan Barrett of self love I'm gonna reach into the camera and give you a hug of love if that's okay I'm asking you to turn to someone a pet a teddy bear pillow give it or them a hug of love There's hugs are a great source of love and let's face it We could all use more love in our lives and Amy wants to remind us Trust is paramount exactly beach lover says good job. J. A. Margaret says, thank you Beach wait Sally says Thanks, Jonathan Net detective says hugs power cheese says, thank you Phyllis says live. Yes live chats are good. I Want to thank all of you that donated to the Connor as a scholarship fund I am so grateful for all of you. You are wonderful human beings to be so generous with me and Mostly importantly that I am grateful that I can help make a difference in many of your lives So thanks for putting up with my profanity and expletives every now and again I am truly grateful and I just want to give you all a big gigantic hug of appreciation Thanks a bunch wishing you all a super duper wonderful fantastic evening


Okay, so we move on to our next presentation. Let me remind you that this year we're trying something new to the questions. So you can log into onlinequestions.org with the event ID 2147 in our room number. And you can ask questions there. I'll vote for your favorite question. So our next presenter, Akil, will introduce QML in the video industry. Please welcome Akil. Thank you. Good morning and hello. I'm Akil. And today I'm here to talk about how in Cadian Live we're trying to create videos out of QML. So let's get started. A bit about myself. I'm a student at Amrita University pursuing my undergrad studies in computer science and being a Cadian Live contributor for more than a year now. I was part of them last summer as part of the Summer of Code program. And I'm a part of AM Force. It's a student community back in my college.  And over the years we had a lot of students taking part in various open source programs in various organizations through these student programs. And especially if I were to say we have a lot of people in the open media, especially in the media field. Just to name a few. Mindfree is Krishna here. Yeah, we are from the same bunch. And yeah, that's that. So let's start with the why. Why would you take QML? A language you would use to create UI, UI applications. QML is something you would use if you were a Qt application developer. Qt is a cross-platform software development platform using C++. So QML is something that you would use to actually design your application interface. QML defines the behavior and how your application should look like. So if I have a button on my window, you can use QML to say where I can place this button and how this button will behave and stuff like that. That's what QML is used for. So QML does this by order. It orders the interface elements in an hierarchical manner. So there's the child parent thing in QML. So why would you take QML? It's like JavaScript. So yeah, why would you take QML to create videos? So that's the end result of what we managed to do in KDNLi right now. That's a QML file, by the way, in the KDNLi clip monitor. That's actually playing. So why do it?  And B, QML is actually awesome. So let me tell you where we do this in KDNLi. We actually do this in the title tool. So in KDNLi, the video editor, we actually have a title tool. So what we use the title tool is to create title clips.  Suppose if you want to add subtitles or you want to add the credits. At the end of your video, you use the title tool for that. So how it works currently in KDNLi is the KDNLi title clip is basically an XML file which describes how it should look like. And what we do in the backend is we actually pass this XML. We pass each of the attributes, text, image. We do that.  So that's a hefty process. And no one uses QGraphicsView. It's depreciated.  And second reason is it's a lot of code. It's a lot of lines of code. And yeah, those two reasons why it is not really that nice to do this. So the second reason, yeah. So why use QML? Because rendering, let me tell you how rendering works in QML. So since QD Quick 2, rendering in QML makes use of the cute scene graph. Now the scene graph is a common term in the rendering world. It's a data structure which basically represents how the QML item scene looks like. So as I told you, the QML is basically a child parent thing. So the scene graph will actually order this thing during the rendering process. So yeah. So that's what the cute scene graph is. Now as against traditional imperative painting systems like QPainter, this is a much faster way to render stuff because the cute scene graph actually takes lesser state changes to render, say, if you have to render 30 background images. So instead of 30 imperative painting calls, you would just take one call in the scene graph. So yeah, that's how QD Quick Content is actually rendered in the back end. So this is a big advantage because we don't really have to pass stuff. And yeah, so that's QML rendering. So there's a way to actually tweak this process. So now I want you to imagine how we would want to take QML and if you want to make a video out of it. So the nature of approach would be to load this QML in a quick window and then call grab window for each frame. So that's the native approach. You can just call grab window for each frame that goes by. And this one sucks because it takes a lot of time. Suppose your QML file takes five minutes from beginning to end. It would take five minutes to actually render the whole thing because this thing is like screen recording. It will take each frame. And to grab window is expensive. As you all know, it calls the OpenGL graph pixels. So it's expensive and it's time consuming. So how we can tweak this to make it a lot more faster is to make use of this magical class called QQ render control. So as the name says, render control, it allows you to control the different steps in the whole rendering room. So now when you use this class, you don't actually need that window. You can actually just tell the renderer to actually render your QML onto a frame buffer object which you can just use it for your image later. So that's what QQ render control allows you to do. So that's what we have tried to do. We have tried to load a QML file. You use this class and directly render this QML onto a frame buffer object and save it. And I'm not going to go too much into technical because it might get a bit boring. So when you do this, you're getting obviously a lot more freedom to do it. But you will have to handle the context and the frame buffer object and threading and stuff like that. So that's that. So the stuff I just talked about, we got this idea from this blog. So you can just look it up if you are really into technical details by Andy on the Qt blog. So it's a great blog how Andy actually observed that people were using the Qt API in ways that they weren't supposed to. And he just sought out to make an example out of that. And that's the result. So you can find the code there. It's I think production ready. That's the QML. I call it the QML renderer. It's a library. It has you can actually even test it out on. You can just go QML you go the input file with the QML file. You tell how many frames you want. And you can just give the upper directory and you will get those many frames in by default be JPG or whatever you want. So I can even tell which format you want. And yeah, so what now we have the frames. So most of the heavy lifting is done now. Now we have taken a QML file. We have rendered it. We got the frames. Now we just need to, you know, combine it together and play it. So yeah. Now MLT. So kid in live uses as its back end it uses an open source multimedia framework called MLT. So MLT was originally it's a it was made for broadcasting services. So it's loosely based on the design on the consumer producer design pattern. So what we need here is we have some QML frames and we need to, you know, have MLT producer. So in order to, you know, play that QML file. So yeah, it's not that hard really. I just this was done around a month ago and we right now we have an MLT producer which can actually play a QML file. So, yeah. So you can find the code there for the MLT producer if you want to tweak play with it. And yeah, it's the demo. Yeah.  Melt is the command line interface for MLT. So you go just you just tell melt which producer to use. That's QML and you tell the input file. So that will just, you know, tell you to tell. There you go. It just plays it there. You can see the you can see the frames changing there. So that's that's how MLT does it. And yeah, so we are done with QML. We are done with QML rendering. We got the frames. So we got our we are able to play it. And the next process, the next step. Yeah, we have actually tried to try to put that in Cadian live. This work is currently ongoing. And what we've done is let's just connect the producer to the clip monitor in Cadian live.  So you can just play the file. That thing doesn't move, but it's playing. So yeah, got so far. This is still in process because we are not there yet. The QML, the MLT producer can't yet animate animated QML frames just static ones because for our initial testing, we thought let's stop with this and it works fine so far. And yeah, moving on. What's next? So yeah, as I said, we need animated QML support. We need that's an obvious requirement if we were to create a new title tool in the future. And QML obviously having animated QML is an requirement. And yeah, once we have that and once you've completed the integration of the current producer and we're done with testing it completely with Cadian live because so far facing some issues with handling it in the timeline. And we'll be looking into that soon. But once that is done, I suppose we could just move on and move on with the title tool. So this is what I did for my last summer of core program. And yeah, so last summer I worked on creating the QML render library. And after that the work was to create the MLT producer and which was done a couple of months ago. And right now we're into creating the we're into integrating this producer into Cadian live and and yeah, once that is done, we will actually start to think about how we're going to design the new title tool about the about the interface about how we're going to change it about what things we're going to add. And after that is done, we can actually possibly get it done and maybe or maybe I don't know in some time, I can't really say. And yeah, so I guess I'm I'm really done. Yeah, so if you have any questions. You have one question online. So on online questions.org. And it is, do you think this approach could be used for 24 seven live play out system? Well, really, because you could, because you are really creating frames, but I'm not sure about the performance. You could, in theory, create an MLT producer to do that or something else, which is more feasible. But so far what I have noticed is it's not really that quick the QML renderer. But in theory, you should be able to do it because in the end you are just creating frames and you just need to play them. So I'm not sure about the feasibility, but maybe next question. How do you manage video rendering time on QML side? How do we manage Q video rendering time on QML side and really get that question. Well, we have a suppose if we have a static QML frame where we really don't have animations, you don't need to worry about the time it takes. Rendering time. Okay. All right. The time it takes to render, I guess. If you want to start an animation of this frame or this frame, how do you make it? Oh, all right. All right. This many frames? Okay. Okay. That makes sense. Yeah. All right. Yeah. Well, the approach right now, how it works is we just, there's an animation driver. So we have actually, we have a custom animation driver, which we have overwritten. So if we want a certain frame at a certain point of time, what we do is we just advance to that point of time and we get that frame. That's how we do it right now. That may not be efficient, but we are getting there. Any other questions from the floor? Raise your hand. Title sequences. Do you see this potentially something suitable for like hold the shoulder graphics or like banners?  So the question is, yeah. The question is if we can use this approach to actually, for more, a wider range of applications such as presentations or banners or stuff. So the answer is definitely yes, because you have the entire QML spectrum, which you can use to design stuff. So I don't really, I didn't really face a limitation till now because we are rendering using the scene graph. So there is no limitation per se of what we render. So if we can render QML successfully, we can actually connect it to anything we want to. So yeah, the answer is yes. Another question? Thank you, Akil. Thank you.


This study found that both CMV and EBV can cause changes in the number of certain types of T cells in the bloodstream. These changes are most pronounced in older adults who have been exposed to both viruses. The study also showed that these changes may be amplified when people are infected with both viruses at the same time. This article was authored by Mali Zayhovsti, Alper Savergil, Marilena De Zebrower, and others.


Welcome back to my channel guys. It's your girl Coco styles and on this channel. We talk about beauty fashion lifestyle and styling Let's get into some 2024 spring Things that tall women should be wearing I was doing some research and just trying to find other brands for us to shop at and You guys everybody know the struggle is real, but you also know that I make regular brands work for me And I'm also the girl who buys pieces that are of Great quality so that I don't have to keep buying it over and over in season after season So as tall women we definitely have to start shopping way before the season gets here That's solely because a lot of the average high girlies are grabbing everything And it just takes us a little bit longer to find things that are stylish that fits our frame and For me and I know you guys are thinking the same thing You don't want to be left out of certain trends or just things being out of stock because of our size and again Most of the average high girls are wearing like oversized things That may fit us regular if that makes sense So I wanted to do this video so you guys can start jumping on the trends now and what you guys will be seeing this 2024 spring I'm gonna pop in some photos of some things that I saw online on some of the fashion Websites and stuff or just random photos, but I also have a lot of the pieces already Which is why I always say choose quality over quantity any day 99% of the times these pieces are coming back and forth through fashion through the years And if you bought something that is of great quality You don't have to buy it again, which leads me to the first trend that you guys are gonna be seeing that you've already seen and Those are shorts Okay, there's a ton of shorts out that you could actually wear. I would say For me, I'm a leather short girl Period, okay. I do have some trousers shorts, but they are more of a they're not like a short short They're like an average length, but short shorts are in now if you're a girl who does not like to wear short shorts You can always wear a pair of tights with it Wear a longer shirt with it have the shirt open something that's gonna make you feel comfortable The other thing you can do is you don't have to always necessarily wear a pair of shoes with it like heels because heels can also make the shorts Feel shorter make you feel a little bit self-conscious But if you do not care about any of that Definitely pick you up some shorts. They have really short shorts now I'm not even sure what they call those things from back in the day. We used to call them panty shorts But they're very very short girls are wearing sheer over it or even lace so that you could actually see the silhouette of the quote-unquote panty shorts Or they're wearing like really really long tops.  If you get something that is very on trend. You might have a little bit of difficulty trying them Now match it up in like a future Year season if that makes sense. So shorts is the first thing if you don't have shorts Definitely get you some shorts for this spring. So places that I like to shop for shorts I would say check out reformation and end of the stories They're not tall girl specific But you guys have to really start reading these labels in these details because you would be surprised at how many Items can actually fit our frame without looking off if that makes sense So read the details if you're shopping online If you're in the store, make sure you are reading those labels the second trend for 2024 tall women should be looking into our High-rise trousers now you guys know how I feel about high-rise pants on tall women We it doesn't necessarily always look the best on us because it's really making the bottom up us look even longer And I would top torso look shorter. So when you are buying something like that try to balance that out Balance it out if you're gonna tuck your shirt in have it like fluffed out a little bit If it's a t-shirt just really try to balance it out if you wear something that is that is tucked in that is fitted More of like a tank top Balance it out with something that's gonna take away from them looking extremely long at the bottom and your torso looking really short Now there are girls who actually do have a shorter torso So they have to figure out ways to make their clothes look balanced on them And it's the same thing for these high-rise trousers But if you do not have high-rise trousers for me, I can wear a thirty four and a half thirty four Those seem to be okay with me. I can wear a slightly small heel If you are taller than I am you're gonna have to get something a little bit longer So I bought mine from favorite daughter. They go up to a thirty four, but there's about an inch and An inch that you could actually bring down They're high-rise. They're pleated. They're really nice. If you don't like double pleats. They're not the pants for you However, you could actually go to your tailor and have them remove one of the pleats and kind of bring them over That's what I did because it was giving me like a bulge in the front But they are very very great great quality other place you can check out is frame Read the details guys because like I said some of these designers do sneak in a pair of two a pair or two that the inseam is like 36 so Do your research don't get stuck on just specifically shopping at tall girl brands because if you do You're literally gonna be dressing like the other tall girls out here. Like I don't want you guys to Like do that if that makes sense. Like they're there, you know Some girls they have to do it but get creative if you really like to look different from everybody else It's not that many people who do fashion or who love fashion or who just love to look nice and put together Do a lot of different things so especially for us tall woman. We are limited. So Check those out Read the labels Read the details See what works for you and get you a tailor you guys already know how I feel about a tailor a tailor is your best friend Whether it's a shirt pair of pants pants. You want to get crop jackets. You want to get crop get a tailor It is your best friend. I'm probably always gonna say that Find you a tailor and you're golden So the third fashion trend that I'm seeing for 2024 are roses now I'm not a girl who loves the tons of roses all over my clothes But I did see some really nice pieces that had like single roses Maybe the skirt had a lot of roses on it but not so much the top So if you don't want to go like overkill was an entire outfit full of roses Then get you something for your neck like a choker Maybe get something that has like a rose Like a rose on the shoulder of a shirt Something like that if you don't want to get anything that is like flooded with it Definitely try to find something that has the least amount of roses and go from there now Don't get me wrong There are some dresses that are like fabulous that have a ton of roses on it and I'll pop in a photo um but Sometimes it can be a bit overkill for some people But if you're going to an event and you want to stand out definitely get something like this Otherwise get something that you can put around your neck That is a little bit more subtle when it comes to wearing roses this spring if you like this video so far Make sure you are hitting that like button So the fourth trend that I'm that you guys are gonna be seeing in this spring that I think tall girls should dip their toes into Would be sheer skirts Transparent skirts whatever they want to call it now if it's a little risqué for some of you guys But they are again wearing those shorts under it and wearing like longer jackets. So it's kind of like wearing a Bikini bottom I guess you are a little risky You can definitely go for that or you can go for something that will kind of take away from it looking so Risqué for me the color black always looks a little bit more sexy So wearing something that is monochrome with that type of style You may be able to get away with it and not feel so like naked I guess So definitely check out transparent or sheer skirts for this spring Again, if it's not your thing go for something else Maybe a pair of pants and that you can wear like shorts under it Just get creative if you really want to jump into the trendy thing this spring We can do it girls. We don't have to be stuck with the boring clothes because we're tall. I'm sorry.  I will say For trendy pieces, I do not recommend spending a ton of money Because it's not gonna be in style for the next Season all right, so think about things like that when you are purchasing something that is trendy Always keep that price in mind and keep that cost per wear in your mind guys you already know how I feel about Wasting money and not being able to wear something that's great quality for multiple wears in the last trend That I wanted to bring for my tall girls would be the 90s vibe casual attire So it's like casual business attire which has been out for a while now so again favorite daughter does have nice trousers and They're about a 34 inch I think they they're 34 with the inch so that's 35 in total, right? She want to get like really nice tailored white tops The Frankie shop has a really really cute one and guys I screwed up the other day It was in my bucket because it came back in stock But your girl Freaking got sidetracked and and now it's going back out of stocks get you some nice white button-up shirts You can get fitted ones. You can get oversized ones. You can throw on ties with them Get creative for your spring wear guys again The struggle is real for us tall girls But we don't have to be excluded from the trends because guess what models are tall I just don't understand why they don't make things that fit Tall women they make things for plus size room, but they definitely leave us out But I'm here to help you. We don't have to be Out of the trends and stuff. We just have to make regular brands work for us That's it. Those are five things that tall girls should be wearing this spring That's on trend. So if you guys want to find out More of the pieces or styles that's gonna be on trend for 2024 spring definitely check out Vogue Check out some of the insider Magazines they have a ton of stuff and just make it work for you Every trend is not for our frame and we already know that figure out what works for you Find the item and start to slate in this spring guys So thank you guys for tuning into this video make sure you are commenting below and liking the video and subscribing to the channel guys I really appreciate it. So if you stayed to the end of this video, please comment Coco we see you and guys don't forget to follow me on Instagram at Coco styles NYC and Coco styles and why and my style blog Which I have a ton of tips and I'm gonna be adding in some more lifestyle stuff over there So you guys can check that out That is Coco styles NYC and my personal style services Coco styles NY calm and I will catch you guys in the next one Bye guys


from I.T. Rootkey that is set in at the fourth house of Himalayas and it is in north of India and it is actually the oldest technical college in India which was set up in 1853 and at that time it was called Thompson College of Engineering. Anyways, this summer I have been working with Dr. Webukai at the UW Carbon Cancer Center and I have been working on molecular energy to target tumor mangyogenesis and we have used antibodies as well as protein nanoparticles to do the same. Now, cancer as we know is the second leading cause of death in USA and the major problem that accompanies its treatment is its late detection. By the time it is detected it is already so way ahead into, I mean it is like way ahead into the disease so it is here becomes impossible. So, this is where molecular imaging can help us. Molecular imaging is the visualization, characterization and measurement of a biological process either at molecular or cellular level in living systems. And these imaging modalities these include positron emission primographies and optical imaging which includes fluorescence, vial fluorescence and magnetic resonance imaging etc. Now, to improve upon these imaging modalities either in terms of improving upon the imaging levels or just changing the imaging levels totally we can use nanotechnology which will help us in early detection as well as in accurate treatment of accurate diagnosis and treatment of cancer or even other diseases. And I think the place of molecular imaging and therapy and nanotechnology the Kailat works. Now, these are some of the small handle molecular imaging modalities that are available which includes optical as I said before MRI, PET, CT that is computed tomography ultrasound imaging and spread and the University of Wisconsin has 4 out of 6 of these which have been used for research. Now, the aspect of cancer that that microjet that microjet would be targeting is angiogenesis which is formation of new blood vessels and this is very important for initiation and progression of cancer. One of the proteins that are expressed mainly in the vascular endothelial cells during angiogenesis in proliferating tumors is CD105 and this is a transmembrane protein. So, this would be our target. The targeting ligand that we would be using in our project is TRC105 which is the monopermal antibody and this binds to both human and urine CD105. And in fact it is about to step into second phase trials in USA, clinical trials in USA and because PET is such a great imaging modality in fact it is one of the primary imaging modalities for tumor detection and due to its excellent sensitivity tumor penetration and its ability to be at the images of PET can be accurately quantified. So, we will be using PET that is positron emission tomography as one of the major imaging modalities for microjet. Now, generally like PET as of now in our clinical oncology it uses fluorideoxy glucose which is a which is the glucose molecule which is bound to fluorine, a radiolabin fluorine as you can see over here and this radio tracer is now injected into the body intravenously and where the this fluorine the PK-Soli emits positron, positrons which annihilate with electrons and release gamma rays which can be detected and this is how a tumor is imaged tumor or in fact any disease can be imaged. But the problem is that this FDG it is based on fact that it would be incorporated in glucose metabolizing regions and cancer as cancer has been shown to be a highly active metabolic tissue for glucose and FDG is not cancer specific. So, this can have high updates in normal organs which normally metabolize glucose very like at a very high rate for example, brain, heart and muscle and also in some conditions like inflammatory now conditions or some infectious diseases or after surgery the tissues in those regions they show a high update of glucose. So, this might result in false positives. So, that means that so we need some imaging modality or sorry some imaging tracer which would be very specific to tumor. So, this is where we use antibodies. Now, why do we bring in graphene at all which is nanoparticle? The thing is that nanoparticles they show they have a large surface area onto which several imaging labels can be conjugated. So, this would give a high signal dramatic signal amplification also we can attach different targeting ligands onto the nanoparticles. And so this would increase the binding affinity and specificity of the constructs and also nanoparticles have been shown to bypass biological barriers even the blood brain barriers in some cases. So, the targeting efficiency is enhanced and in future what can be done or is postulated is that a single nanoparticle can act as a substrate for holding the imaging tracers the ligands as well as the drops. So, that you can have simultaneous imaging diagnosis and cure and treatment of the disease. So, if that happens that would be like from some people who have read people's nanotechnology can is actually the only answer probably now to effectively deal with cancer. So, with this overview I would like to introduce you to my projects. The first one is we will try to target vascular tumor vascular using functionalized looking outside nano streets. And the second project would deal with developing an antibody which dual modality agent for a pet and optical imaging in breast cancer of 41 cells of breast cancer. So, moving on with my first project we have this protein outside nano sheet on to which we would conjugate the TRC-105 which is the antibody and on to which we would be conjugating a radio tracer using this is NOTA actually a collator for radio nucleates and so I will move on with my project. So, first of all with groupine what is groupine? Groupine is actually a monolayer of carbon atoms it is a two dimensional sheet in which carbon atoms are like take the form of hexagons and this is a very amenable nanomaterial because it can be easily wrapped up into nanomaterials or can be rolled into nanobus as you can see in this animation here and it can be styled on one or these can be styled on top of each other to form the fight and actually it has been very much in use since it won the Nobel Prize in 2010 when it was discovered and but it has not really been used in biomedical sciences as such it has mainly found uses in electronics. So, it is very easy for nanomaterial to work with because just like apart from the high surface area which all the nanomaterials provide it has also been shown that groupine is as the high believer stability it chose low toxicity and it is very easy to functionalize because it has a plane sheet like structure so it would be very easy to attach things on to it. Now this is my workflow so what we did was that we took groupine of side nano sheet which was around 10 to 15 nanomaterials in dimensions and we conjugated NOTA which is our biopunctional calculator for radio nucleolucreants on to it then we attached the antibody which would be our targeting ligand on to it. Now these constructs were then tested in mid-flow in VUX and by microscopy studies and facts analysis and after the successful like we got some good results with it and so we now in we like we did copper 64 which is the radio nucleolucreants for PET imaging on to it and these constructs were then intra-venous injected into breast tumor bearing mice and and we image them at different time points from 3 hours on to 48 hours and then the mice were sacrificed and biodistribution was carried out using gamma counter to validate the PET data. So, this is the first step where we conjugate NOTA on to dripping of side. NOTA is basically a trihazor, cyclononene, trihecetic acid, this is a macro cycle and the compound of NOTA that we use is actually benzyl isothinate derivative isothiocinate derivative of NOTA so that it would help us in like you know attaching it to dripping of side nano sheets. So, these dripping of side nano sheets are also functionalized with PET, PET would help us in to with help would help to act as a spacer between dripping and NOTA and similarly we attach the antibody to dripping of side using PET again and then we carried out facts and fluorescence microscopy studies the results I would not be able to show them to you, but so then we carried on with copper labeling and this NOTA here it so when we incubate with copper at the particular pH we see that the copper atom it goes and fits inside the cavity formed by the bridges or carbon bridges and the nitrogen atoms and these ones that you can see over here these are actually coordinate bonds so the bonds are pretty strong and so this is how we label it with the protein conjugates and now these conjugates are injected into a velocity in mice and as we can see these are the PET immigrants at 3R time point and 48R time point and the control would be just a plain graphene oxide nano sheet without any targeting antibody that is TRC105 and we can see a very good very good localization to the tumor in case of the conjugates but in case of graphene oxide we can hardly see any tumor uptake so this shows that the graphene oxide and the graphene oxide and TRC105 conjugates have a very high tumor like comparatively high tumor uptake and with time at 48 hours you can see there is a diminishing of signals so that is probably because of one copper 64 it has a definite half life of about 12.8 hours and so this starts decaying so the signal would diminish yes and also the conjugate starts getting eliminated from the mouse so this was basically the first project and that had completed in here and so we see that the TRC105 conjugation to graphene oxide it increases the tumor uptake also PET has a lot of good quantitative and non-invasive measurement of graphene oxide conjugates in the living mice and if we can further develop it we even need to perform some more experiments like some blocking experiments to confirm that graphene oxide and TRC105 conjugates are actually being directed to the tumor because of CD105 because of specificity for CD105 and not because the tumor that's the nature is just leaky and so antibodies tend to like you know get easy get into it easily and so if this thing can be developed further on so in future we might expect some image-rided drug delivery also from these conjugates or similar conjugates now my second project was development of antibody-based dual modality PET optical imaging agent in which we used an antibody the same antibody TRC105 the main reason why we want to do dual modality imaging is because as of now we do not have any modality a single imaging modality which is perfect in itself and would be enough to answer all your questions about diagnosis of a particular disease for example optical imaging it is it has good spatial resolution but it is very difficult to accurately quantify the fluorescence in our signals that we get and also it has two additional penetration with PET which is very sensitive and can be very easily quantified the problem is that it has poor spatial resolution the images are not very well resolved so if we can combine both of these we can get complimentary


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DigiKey and Adafruit bring you I on NPI that's right. All right, so this week Lady Aida we are going to show I on type C USB connectors This is actually a character that I've been hoping would exist soon I couldn't find it but then I saw pop up on digikey.com NPI their third new section and I was like, yes I want this USB Z on every board one. I have this one cable thing. It is what I want Okay, so what is this thing? So it's a USB type C connector and I wish you had a really good guide on USB type C it's a reversible connector for USB and The thing that's really cool about it is it can do like like fire wires type speeds It can do like plain USB. You can do up to 20 volts It's kind of really mashed everything into it The problem with that though is that to mash all these capabilities speeds power, you know, all that stuff There's like 24 pins on the typical Type C connector, which is quite a lot to solder So what I really like about this connector is it's a power only connector from Kui So if we look at the diagram You see it only has six SMT pads on it because they basically only connect to the power pins the V bus pins the ground pins And they have the CC1 and CC2 pins available But it's like super simple to the simplest type C connector you can get if you only want to get like power into your device so What's nice about this is that we can compare it to you here's another Kui part a 24 pin connector and I got a 3d rendering of it I also showed on the overhead and it's just got like a massive number of pins and so it's not really hand solderable It's not really workable like those pads are underneath the connector and also the cost is much higher like for character like this You know, you're gonna pay like $1.50 or $2 per whereas the power only connector is only like about 50 cents in quantity So let's go to the overhead We can take a look at these it's a simple and PI But you know what like if you need this kind of connector, you'll be like this is what folks are here for me They're like, yes, one. How do I find it? Where do I get it? What does it look like? So this is You know another connector with it's USB type C Also, you can see there's the USB type C, but it has every contact brought out and again It's gonna be a couple bucks because you have these inner connectors here then these outer connectors Everything is available You know the high speed lines the low speed lines all the power pens that Sideband pans. This is great if you're making, you know to invite advanced peripheral or a You know a computer that has to be able to handle all the different kind of peripherals plugged into it This one in comparison you see it's not only that it's smaller too. So you need less board size It's like the size of a micro Be connector and you only have the six pads to them a power to them a ground And then you have the cc1 and cc2 pins and the reason you need those pins is that you have to connect Resistors to them to indicate to the upstream power supplier. What kind of power you want? So we recommend you copy our schematic For two five point one K 1% resistors connect one to cc1 one of the cc2 connected to ground and this will basically tell the USB type C Controller, hey, I want you to provide me five volts, which is kind of standard if you want higher voltages It gets more complicated you get to kind of like negotiate the power delivery Specification, but if you just put these resistors on like bam, you're you've got five volt power You know half an amp to one and a half amps depending on what can be supplied. So super easy To use I really like this connector great price easy to use simple. I like the foo hole Connectors through to the PCB because that keeps it mechanically stable and some big chunky pads for power All right, so what do they get it? So you want to pick these up? These are new from CUI. I call them kui, but also just say CUI It's uh, you know, this is the part number. It's the 2223 UJCHP dash three SMT And then CT for cut tape ND is for digikey. So Oh, you go to the short URL and pro tip when you're on the digikey site There's a little share thing and it generates a short URL. Yes digikey.com Ford slash short Ford slash Z HT BW five and that's great because you can cut and paste that from the site Yeah, send it to yourself. I always drop it into like a text file or I'll drop it to something that syncs with my phone I'm gonna go look at it later Send it to you. You know, there's all sorts of things. Yes, so Available now go get it. Yeah now from and you think now that you know it exists when you do your design next time You do a design and you're like, well, I just want USB power I don't need data because it's you know, just something that's powered off of USB This is a great connector to source and use so you know, it's there for when you need it And that's on MPI