What is going on here dude? G here welcome back to another Roblox video today. We're gonna be playing dead silence this sewer Now as you guys can see this is a scary game. Oh god, okay. Well, it seems like it's gonna be been tense. So let's begin. Oh God Okay, so I said the sewer so has to do something with sewers that is nasty I don't know why to be in the sewer. Okay. Oh, and here we are in the sewer Okay, so where do we go so dark down here? There's a ladder. Can we climb up here? Nope. Okay, I guess we can't go up. So we're supposed to go down here in this dark place Okay, let's go explore I guess. Oh when we're joined by Rainbow Nova Star. Hey, what's up, man? I guess it's just the two of us down here. Oh my god. Oh, there's a note Okay, if you find this message you are probably looking for a merry shop, but good luck though I feel like it's just a hoax to scare people off. So it seems like we're looking for this merry shop person She might be lost or something. Maybe she just wandered off and she got lost in the sewers Okay, this is gonna be very spoopy, man Okay, oh god Okay, what is that some logs? Okay, I thought it was something else. Let's just keep go Now light just flickered our flashlight is flickering man. That is not normal Okay, let's keep going this way. Oh God What's over here? I know we saw a vent back there, but let's go check this place out Oh, there's a toad here. Hey mr. Toad or frog whatever you want to call it. Keep out wait you I need to find another way around. Okay, so it seems like we got to a dead end here So now we have to look for an alternate route to get out of here, which there was a vent back here So let's go check that out, man. Hey, what's up, dude? Okay, so let's keep going this way. Oh My god, okay, so we're supposed to go in the back. Oh Enough we go into the vent, right? So let's go in here. Okay. Oh, so now there's train tracks. Oh my god, what is this place? It's all dark and it's a little scary Okay, oh another dead end great What this place is just pure dead ends?  Don't worry. We'll make it out of here alive. Well, I have reached my destination Okay, so we go up here. Okay, so now we're in the vents There's been a nappy entity scary here man. Let me put my volume out of the way up That's where I like it. Okay. Oh Um Wait, that's the way we were supposed to like well the way we were gonna try to come through It was just blocked off. We just came around We just fell into a pit. Okay. Well, we're in a place now.  Oh, we just hit our heads on the ground seemed like somebody was trapped here Oh, my name is Eric. I've lost track of the days. I've been here My mind tends to wander far and deep as the ocean as I sink in the water deeper and deeper until it gets nothing But darkness do not look do not look don't look don't look back Oh god, okay seems like Eric went a little cuckoo here. He was counting the days Let's see how many days was a year five ten fifteen twenty twenty five thirty thirty five forty forty five fifty fifty five sixty sixty-four sixty eight Sixty oh my god, he was here like around seventy days. That's insane. I would have gone crazy too, man Okay, so oh my god, it would sell for Dude, I just heard some footsteps and there was not him. Okay, so can we go in here? This door is locked Okay, let's go this way, man. Oh Great, isn't that just magical our flat slide seems to be ticking off. Well, I hear flies Something is blocked from something is blocking the other side Dude, you guys hear those flies That is nasty. I do not want to go through that door control room. Oh god Okay, there's curtains. I know something to pop off from there.  That was your name, right? Mary. I Mean no harm to you. Okay, where'd she go? She's just vanished. Oh My god, okay the washroom. There's a strong order of dead bodies in here. Oh god. Okay. Let's get out here We did not want to go in there. Oh No, Mary's lurking around here. She looks pretty creepy. Oh My god, please don't pop out.    We don't want that to happen Okay, so we go up these stairs Whoa, it's like a control room here turn the power on. Where do we do? 093 093 532. Oh Well, you just did something buddy. Good job Good job, buddy. You just opened the door. So I guess we have to go back. Oh My god. Nope. I know there's something gonna pop out. I Know something's gonna pop out here No, okay, whatever man. Let's just keep going wait. Did we just go in a circle? Well, no wait. Oh, I think it was this door Door is locked. Wait, we just went a different path, right? Where is it the same pathway? No, it's the same path I guess we're supposed to go back because he we did open cell one So now we have to go back to cell one. Oh my god. It's the door with the flies. Oh My god, dude Oh my god, what is going on here, dude? Oh No, buddy, this is not good. Hey, move out the way man. Oh My Jesus Okay, you guys, what do we do here? Oh for them. Yeah, that is the right way. Oh my god. Excuse me mister Well, sorry, I just want to get out of here Rest in peace to them. Oh god. No, please don't do this to me right now. Oh No, there's a dead body right here. There's bodies all over this place Oh my goodness. Oh Okay, so I'm just trying to try to get in here. Let's get out of here man. I'm out of here boy You're on your own now Okay, just try to keep up man, dude. That was so scary. Oh No, wait, we're in the same room now. We just have not to try to fall down here Boy, there better not be something right here. I swear. Oh My god, please don't fall That's where they're better not be anything popping out right here because I'm gonna cry if there is Okay, nice. Now we're in the vents It's where oh no The better not be any scary in the vents Okay, everything seems good so far. Okay, let's go Open sesame. Okay. Now what control room So now we're what is this place? I can't make of this place. Wow. It seems like it's like a hospital or something I want to say But maybe it's like an asylum. Oh my god. Okay. Now this is scary Of course, this is a dark hallway Oh my god, hopefully we're going the right way door The door's locked. Of course it is Okay, this door is open Oh my god, you go first man Okay, let's just be cautious We got this man open the door This door open the door is too rusty to open.  She's in the window Dude look at her Dude that looks creepy He just disappeared Oh no, okay. We're going into the basement. What the doors too rusty to open But where are we supposed to go? Okay, we can't open this door So we have maybe we went the wrong way But maybe we have to walk back. Hey, what's up Hayden? Door's too rusty to open. Oh, I see this little crack here in the wall. Oh my god So now the guy left me alone. I'm here all by myself That is not good Okay, we just opened the door now Hayden Help me buddy. I'm too scared I'm out here by myself now. Oh my god. I see a shadow Mary is that you? Oh my god, there's mannequins. Okay. This is gonna be scary now. There's mannequins and we have to walk past by them Oh my god, here goes nothing This is kind of creepy. Yeah, man.  This is so scary Oh, no, there's so many of them. What is this gonna stop? Oh god, please don't move.  At least those mannequins didn't move We have to walk back. Are you kidding me?  Okay, let's just be careful Oh no, I know Mary's gonna be around here somewhere. Oh god. Why do I hear the flies again? Okay, so the basement door is open guys We can go this way now Oh no, Mary was Was back this way Oh god, this place looks scary Hayden just left. I bet you he was scared to like catch up to me Okay, I know the basement door is open. We're in here, but the flashlight, there you go We couldn't see a thing Wait, are we supposed to go in the sewer? Cheesy amigo, what's up, man?  Oh no. Oh, I see the light There's a light at the end Get out of here. Well, what do you think I'm trying to do, man? Oh god, here we go, man. Here goes nothing Oh god, wait, what happened? No, we're stuck. We can't move. Oh no, it's Mary What is she doing? This is not good. Wait, what happened? Playing our teaser leave a like if you enjoyed the game. Wow, this was a teaser Well, this was like the most excellent teaser ever. That's the end. Well, I guess we didn't make it out alive. What? Oh my god, that was pretty scary.  Well, that was Dead Silas the sewer and Roblox I'll leave the link in the description for you guys or in the comments So you guys can check it out because it isn't it is a pretty good teaser or game Whatever you want to call it But thank you guys for watching. Thank you guys for the amazing support If you guys enjoyed the video make sure to slap the like button if you're new to channel Make sure to subscribe and turn that notification about and I will see you guys in the next one. Bye


I'm out of here man! Hey guys, Realistic Gaming here. Today we're going to be playing part of EXE Part 3 since we have played the first two parts of the game. Why not play the third one? Okay, so let's see what's going on here man. We got Subject Zero down here. Oh god, what is he even doing here? Hey there, finally you made it to our party. Okay, so we made it to his party. Welcome to party EXE 3. I am Subject Zero, well we know who you are man. After you destroyed the virus in party EXE 2 we knew that we had to repay you. So we built a beautiful world for you to play around in. What? Okay. But before you're in party there's one more thing I gotta tell you. I'm just gonna be straight with your kid. All right.  Oh, what? So resets every time we... No one will remember you, not even me. Oh gosh, that's a little harsh man. How do I know this? Let's just say you have some experience with Timeline so he time travels, huh? Well I'm just missing hope I didn't scare you too much. All right, he had him there for a second. Here's a quick tip for you. Use a saxophone to get out of here. But remember it won't work when you're talking to other people. That would be rude. Oh god, okay, so what? He just vanished like an enderman. Oh gosh, he said he's our saxophone. Traveling saxophone on a spawn. Oh, you are transported to a familiar place. Oh, we got transported into the world. That's pretty cool. All right, so let's begin with this. Ambassador. He's calling him an ambassador. Okay, so where do we go? There's a door back here, fun times. Let's say it's fun times. Oh, okay, that gave us some damage. There's nothing in here. See, what? We just reset. It's your party's up, dot, dot, dot. Ha, just kidding. All right, so let's go over here. Let's see where this goes. Okay, so how do we get over there? Maybe we go this way? Okay, so let's go over here. A skeleton sets to start here. Trust me, there's a way through this maze. Stay determined. Oh god, so there's a maze here. You gotta be kidding me, brother. Okay, so where should we go? Chapter one. I'm gonna go with the middle one, man. Just go with your gut. Let's see where this takes us. He said there is a way out. We just have to stay determined. And I think we took the wrong way. Oh, we found it. Okay, so let's go up here. Ha, ha. Ha, ha. Oh! I'm out of here, man. Welcome to my Zen garden. Do not step on the flowers. Okay, what flowers? Oh, up here. Treasure up ahead. Uh-oh. Seems like we found ourselves a treasure, man. Oh! What? Just got like teleported, man. Okay, so do we go to the flowers? The sensors detector movements. Remember, he did say do not step on them and we did step on them. A rainbow-colored gas fills the air, not unicorn forts. What?  That's subject zero over there. Uh-oh, I think just got ourselves into some trouble, you guys.  What are you doing here, man? Oh god, uh, let's go up to him. I'm a savage, what can I say? Okay. He just vanished. What in the world is going on here? Okay, so he gave us a red key, so where do we jump back? We jump back this way? Where are we going? Oh my god, I think we're trapped here. I think we were supposed to teleport or something, but it didn't help, and it didn't happen, I mean. Somebody help! Don't tell me I have to reset. Oh, I know what. That's how we got the saxophone. Okay, she said he was gonna add some staircases. I wonder what he meant. Oh, there they are. That's what they, okay, I see what you're talking about, buddy. Thank you so much for this. Oh, nice. Oh, so now since we have the red key, we can go through these. Hey, look at this, wow. It's like we can just walk through them. That guy had no arms. What? Oh my god. What were his arms at? Party XC has been transported into the cave. Bunga bunga, wow. Okay, is that a cave, man? What is he even doing in this century? Where is he? Okay, so I see the staircases that lead us up here. I don't know where exactly it takes us, but here we go, man. Oh god. Oh, there's a bridge. Okay, so we're crossing over. Oh, we're going to the fountain, I think. Let's go. Oh no, we're actually heading to like this place over here. My god, is that a face? Oh, we fell. No, dude, that is creepy. No, we fell. Okay, I don't think we can get back up here. Oh man, let's just go up here. Let's go on the roof and grab this star. Let's see where it takes us. Hey! Oh my god, there's a lot of people joining. I think they're only joining for the event thing, but we're not doing that right now. Okay, so we're liking a giant slide. Oh, we got this awesome music. All right, let's see where this is taking us, man. Oh god, okay, please don't fall. We cannot afford to fall here. Okay, so this is like a speed run type of thing, but it's like a giant slide. Okay, oh, I see the dead end over there. I wonder where this is taking us, man. I mean, we can only find out once we reach the end. Hopefully it's not like a long passage. Okay, so no, oh god. Oh my god, we almost missed that. Okay, so now we're falling into this typhoon. Oh god, dude, we just did a flip. You jumped through the portal and suddenly you're in a familiar place. Okay, where are we at, show me. Special level two, three models slide complete. But, oh, okay, imposter. He's still mad at him. Wait, so let's go in here. Hey, look at me, I can walk through it. He just disappeared. Okay, so we got that star. I still kind of want to go up there. Oh my god, what is that? It kind of looked like the girl's face. All right, whatever, let's continue off of her. I definitely was not a monster. I think he kind of were, man. Farmer, Sam, soul farm. More souls farm weekly. Oh god, I think that's the soul farmer due to the lack of sun, the sun hating green, pellets have returned. The flashlight will help you beat these. I'm pretty sure there's a circle flashlight somewhere. Okay, so it seems like we need to find the flashlight, but I don't know where we're gonna, oh. Okay, jumped, what? Ow, what are we doing? We can't move or anything. Oh my god, we're just taking damage. Oh my god, okay, I think we have to like not take damage from these. Oh my god, this is impossible. No, okay, can you go any slower, please? Let's walk backwards, hopefully it'll help. Oh my god, so this is impossible. Ow, ow, okay, let's just try to do this. Oh my god, please don't. Okay, we need to take this slow, oh no. Ow, ow, okay, no, we're almost like we have less than half our health, ow. Okay, this is impossible, man. How are we supposed to complete this?  No! I mean, I'm pretty sure we were able to jump. We would get it way easier, but it just kept backing us into the air.  Okay, so we went that way, wait, we haven't gone this way, let's go check this side out. Okay, so, oh yeah, it was amazing, we did it already. But there's another door back here, let's get this out. Okay, warning, dangerous round my head, only answer if you are prepared. Okay, that means you all need the best gear. I don't know, let's just continue. Note the round isn't actually finished yet, it will be done soon. Boy, so wait, so this thing is blocked? I believe we can jump over this. I believe there is a way. Maybe this way, we try to jump in. We're trying to break into this thing, you guys, you don't understand, oh, there you go. Oh, okay, here it goes, let's see, let's go in here. And it actually works, let's see if it takes us somewhere, I mean, hopefully it does. Wait, what? We're above the whole map. Oh my God, that's where we were at earlier, where the subject zero's at. Oh my God, look at this map, dude, this map is way humongous, like I had no idea it was this big. Okay, so where do we go? Jump down here, oh no, chapter bonus. Dude, I thought we were gonna fall to our death or something, oh look, here's a pyramid. Oh, I see somebody down there, I see you. There's a desert, dude, it's like Minecraft, they have like the ice, the grass area, the desert, all the different biomes, that's pretty cool, man. And the mountains, oh well, that's like the background. Oh, there's somebody, feel free to drop off down here and explore outside the boundaries, you deserve it. Oh well, thank you, man. Let's see what he's talking about exactly. Dude, look, there's another face right there. Oh, okay, wait, why are we at? I definitely did not understand the space time continuum. Well, neither do I, but here we are. Alright, so what do we do with this? I think we're stranded out here, I mean, there's nothing here, it's so dark. Alright, let's go down here, man. Oh, we're back, the keys to the door should be in a star around here, they always are. Should be in a star around here. Okay, so now we have to look for a star. What? What is he doing here? Wario, what is Wario doing here, man? No, he has a shirt on, Castle IV Tigers. What? Okay, so free health regeneration, let's just touch this thing. Oh, I guess it doesn't teleport you anywhere. So how about here? Oh God, not one of these things again. Dude, this is impossible. This is madness. Oh my goodness, and we just came over, rest in peace. Alright, you guys, so thank you guys for watching, thank you guys for amazing support, this was PartyXE. If you guys enjoyed the video, make sure to slap the like button, hit the little channel, make sure to subscribe, and turn that notification bell on. And now we'll see you guys in the next one.


MS Booster is a powerful tool for rescoring Peptid to Spectrum matches using additional features incorporating deep learning-based predictions of Peptid properties, such as LC retention time, ion mobility, and MS MS spectra. This tool can be used to improve the accuracy of Peptid identification in liquid chromatographer tandem mass spectrometry, LC-MS-MS, experiments, especially when dealing with complex datasets or data generated on an ion mobility separation enabled TIM-CO-FMS platform. This article was authored by Kevin L. Young, Feng Chau-Yu, Kuasi Ito, and others.


It's now 11 p.m. on Friday, the 30th of November. The negotiations are still going on as we left the COP venue, and they'll probably go on well past midnight. People are – the negotiators are trying to negotiate and finish some of the text by tomorrow before the ministers arrive on Sunday. Otherwise, the ministers will have to take charge and resolve all the outstanding issues after they arrive from Monday onwards. So I won't report on the negotiation status now because we don't know what's happening. What I will do is report on a side event that we just completed this evening, which was a joint side event by IIED and the Government of the Gambia, which happens also to be the chair of the LDC Group. And it was about how LDCs are actually going forward with adaptation at the National level, a variety of different experiences from Bangladesh, particularly on how it's incorporated in planning, a review of the pilot project on climate resilience of the World Bank, PPCR, carried out by a colleague of mine, Neha Rai, from IIED, and also remarks from the Government of Nepal, Mr. Thapa, and from the Government of Bangladesh, Dr. Manjul Anan Khan. And it was chaired by Paos Manjul, the LDC chair from the Gambia. Despite the fact that we had a very late night's lot, the side event was from 8 to 10 p.m. We still had a very good turnout of over about 40 people in the room, a lot of very good questions and discussions, which is testimony to the fact that adaptation has now risen up the agenda, is a lot of interest, and there's a lot more interest in practice of adaptation rather than just the negotiations on adaptation. And this side event was a very good demonstration. Tomorrow and day after, Saturday, Sunday, which is the middle weekend of the two-week call, I will be reporting back from the Development and Climate Days event, which this year is being primarily organized by the Red Cross Climate Change Center, and also with the Global Environment Facility, who are doing an adaptation practitioner's day within that, together with IIED as well, although IIED is taking a backseat this year. We hope to have a lot more presentations or practical experiences on the Jeff event, and also some very interesting, innovative actions and activities with the Red Cross event, and I'll report back on them over the course of the next two days.


Thank you for the introduction, and I'd like to thank the organizers for giving me this chance to present on behalf of the TCGA lower-grade gliomas analysis working group on the integrative genomic characterization of this very interesting group of tumors that are shown here. So this is one example of a lower-grade glioma, and this is the pathology. And compared to the normal brain over here, you can see the infiltrative nature of this disease. The background is whiter, and that represents a lot of edema that's being generated by the infiltration of these tumor cells. This is a family of neoplasms rather than one specific type. And the WHO classification of the lower-grade gliomas is shown here. They include astrocytomas of grades 2 and 3, oligodendrogliomas of grades 2 and 3, and oligoastrocytomas of grades 2 and 3. These tumors are thought to be biologically related, but the data for that has not been complete. They're all uniformly fatal, but the time of survival varies considerably from about one year to 15 years. Many of the tumors with astrocytic lineage do progress to glioblastoma. That has been the subject of a previous TCGA investigation, and you'll hear some references to that in my talk. Now, unfortunately, the classification of gliomas is not as simple as this in practical terms. At the ends of the spectrum, the ends of the biologic spectrum, we have astrocytomas of grades 2 and 3, and these tumors are characterized by the infiltration of these tumor cells that are dark and large. They're hyperchromatic. They're irregular in shape. They're known from previous studies to be characterized by IDH mutations, P53 mutations, ATRX mutations, and under the microscope, neuropathologists can prognosticate these and grade them based on morphologic appearance. Grades 2 have about a 60-month mean survival, whereas grades 3 have a 36-month survival. These are the tumors that will progress to glioblastoma and represent secondary glioblastomas when they progress along these lines. Oligodendrogliomas, on the other hand, are characterized as infiltrative tumors that have these round regular nuclei, perinuclear halos, the so-called fried egg or honeycomb appearance. They're characterized by, again, IDH mutations, which you'll hear a lot about today, and 1P19Q co-deletion is kind of their genomic signature, at least, of a subset of them. They also have CIC mutations, FUBP1 mutations, and mutations in the TURP promoter. They, in general, have a better prognosis, grade for grade, than astrocytomas, and it's important to recognize these because they are deemed to be more chemo-sensitive as a group. Now, those oligos and astros that I just shown, I think everybody would agree with their classification. The problem in classification, and therefore, biologic understanding, is that there's a large set of tumors that are called oligoastrocytomas that seem to have both these round regular oligodendroglioma cells and large hyperchromatic irregular astrocytoma cells. There's also a group that are just morphologically ambiguous and well-trained, well-intentioned neuropathologists will disagree on what to call these. So there is a real problem in our classification and biologic understanding of this family of neoplasms. Part of this has to do that we're still using the same histogenic classification of these neoplasms that we've used for almost a century now at the time of Harvey Cushing and Percival Bailey's classification based on presumed histogenesis of these tumors. You'll see that they used terms like astrocytoma and oligodendroglioma and glioblastoma nearly a century ago. Interestingly, there is no mention of oligoastrocytoma in this paradigm. Chances are we'll be returning to this in the future based on some of our studies. So even back a century ago, the authors of these original classifiers said it's impossible to classify these tumors as oligo versus astro under the microscope. They appear just like one another. And a little secret of our neuropathology community is that we really only have 60 to 70% concordance among neuropathologists. So there's a lot of inter-observer variability and in fact there's a lot of intra-observer variability. So we have a problem in our classification and understanding and it's clinically meaningful. That is, there are neuro-oncologists around the country and internationally that have really criticized our histologic classification and are just dying for a better molecular classifier that is more reproducible and clinically meaningful because these tumors that we're going to be discussing are treated in a different way by watchful waiting, chemotherapy, different doses of radiation, etc. So I was more than thrilled when the TCGA decided to launch a study on the lower grade gliomas and many of you are from the institutions listed here. You're well aware of the pipeline that goes from tissue sample to analyte extraction, sequencing, etc.  The goal is the analysis of 500 lower grade gliomas. Surprise to some people is that we've already exceeded that goal of collecting more than 500 lower grade gliomas and they've been analyzed on the platforms listed here. Again, many of you in the audience are from these institutions and have done a wonderful job leading these centers. At the time of the data freeze, which was a slow freeze with some thaws, we had about 293 cases with overlapping data on 254 for the major platforms involved and these are the platforms and the numbers of samples for each of those platforms. So we are in the process of finishing up our first biomarker manuscript and hopefully what we submitted within a couple of weeks. So a very important slide is shown right here and I'm going to go over it in some detail. It is the analysis of significantly mutated genes in the lower grade gliomas and it's been separated into specific mutational classes. So in summary, I think you need to know that IDH mutations occur in 80% of lower grade gliomas. We're going to call those the IDH mutant lower grade gliomas and you can see those are the blue bars, IDH1 and IDH2, isocitrate dehydrogenase right here. These are IDH wild type. Now within the IDH mutant group, there seems to be two classes of mutations. One shown here, dominated by P53 mutations and ATRX mutations, those are mostly astrocytomas and oligoastrocytomas. The other subset does not have P53 mutations but has these CIC mutations, FUBP1 mutations and notch 1 mutations also pick 3CA mutations. These are mostly oligodendrogliomas and they are also characterized by a loss of 1P and 19Q chromosomal arms that will go into. So the IDH wild type tumors are an interesting subgroup. This represents 20% of the tumors and they don't have these characteristic mutations in ATRX P53 for the most part. They don't have these mutations either in CIC, FUBP1 or notch. Instead, they have mutations in genes like EGFR, NF1, P10, PIC3CA as well. Those are mutations that are more typical of glioblastoma and IDH wild type glioblastoma, the highest grade of diffuse glioma. So it's apparent that there's three mutational classes of these lower grade gliomas based on significantly mutated genes. When we look at copy number alterations, we can look at it in a couple of different ways. If we look at it by histology by oligodendroglioma, oligoastrocytoma and astrocytoma, we'll see that there are characteristic 1P and 19Q losses in oligodendroglioma but they're also seen in other histology types. We also see that there's gains on chromosome 7 seen in all histology types, losses on chromosome 10 in all histology types with some clustering. However, if we look at unsupervised clustering shown here, you have really tight clustering of specific gains and losses in specific subsets. So this is, again, just unsupervised clustering and we have gains of 7 and losses on 10 in one specific group. Those happen to be that IDH wild type group of lower grade gliomas that have the mutations like glioblastoma. 1P and 19Q losses correspond to this molecular cluster which is the group that has the CIC FUBP1 and is mostly oligodendrogliomas. And then this group is mostly astrocytomas and oligoastrocytomas that have the P53 mutations and the ATRX mutations. And they're characterized by a frequent 8Q24 amplification event that occurs in the region of MIC and includes MIC. So based on copy number and mutational findings, we did another unsupervised clustering. This is an algorithm called Ancosine developed by Giovanni at Memorial Sloan Kettering and it takes into account both mutations and copy number alterations and focal deletions and amplifications as well. And by employing this algorithm, again, there were three subgroups of lower grade gliomas that came out called OSC1, OSC2, and OSC3. And what we found is that OSC1 was IDH wild type gliomas that had findings of glioblastoma, CDI, and DKN2A loss, EGFR amplification of mutation, P10 mutation, and NF1 mutation. OSC2 was the IDH mutant 1P19Q intact group with the P53 and ATRX mutations, always in the setting of IDH mutations. And OSC3 was the IDH mutant 1P19Q co-deleted that also had the FUBP1, NOTCH1, and CIC mutations. So three distinct subgroups based on unsupervised clustering. Some of the other groups that we, some of the other platforms also yielded interesting results. This is the DNA methylation status. And you'll see that there's five distinct subgroups based on methylation status. This group that has relatively lower levels of methylation is IDH wild type. This is in stark contrast to the GBMs, the DeNovo GBMs that are almost entirely all relatively under-methylated in IDH wild type. And then the other stats include IDH mutant, which have that G-SIMP or hyper-methylator type. But these are divided into P53 and ATRX mutant forms and IDH mutant 1P19Q co-deleted. There's also a very small subset that we find interesting that was relatively under-methylated compared to others. That would probably be IDH wild type as well, but they are not clustered as well in terms of recurrent genomic alterations. Gene expression analysis yielded four strong subsets. Again, these gene expression subsets seem to correlate with IDH status in 1P19Q. So the purple group right here, which is a tight cluster on gene expression analysis, includes mostly the IDH mutant 1P19Q co-deletions. This expression group includes mostly the IDH mutant P53 mutant group. And this group represents mostly IDH wild type. There's a fourth group that was very mixed in its histologies and molecular classes, but it clearly had a defining signature as being more pronural or neuroblastic using multiple types of gene signatures. So based on these individual platforms of copy number, methylation, gene expression, and I didn't talk about microRNA expression, but we came up with four classes using that platform as well, there were between three and five molecular classes, three based on copy number that was very strong and tight, five based on methylation, four based on gene expression, and four based on microRNA. So it's possible to take each of these clusters for each case and do a cluster of cluster analysis to see what the overlaps of these individual clusters on individual platforms would be. And this was a very nice analysis done by Mia Gifford and Sophie Salama, which again reiterates that biologically there are three very tight clusters that are robust and clinically meaningful. So if we look at all the clusters located here on the left hand axis, you can see microRNA, RNA expression, methylation, copy number, and then this group right here is a clear tight cluster, and those are the IDH wild type lower gray gliomas that have the genomic alterations like GBM. These are the IDH mutant co-deletion tumors that are rich in oligodendrogliomas, have IDH mutations and 1P19Q deletions, and these are the IDH mutant non-co-deleted tumors that have P53 mutations, ATRX mutations. So there's three very robust non-overlapping lower gray glioma tumor types. You'll also recognize from this that we have kind of an astrocytoma signature and an oligodendroglioma signature if you will. The molecular data does not support a mixed histology group, so although there may be mixed histology or ambiguous morphology under the microscope, the molecular findings are going to cut these in two. Is this clinically relevant? Well, we think so. You can see the survival curves and progression free survival or event free survival really separate these tumor types based on IDH and 1P19Q status. This is the IDH mutant 1P19Q co-deleted 1P19Q intact and IDH wild type gliomas, and we separate them very nicely based on overall survival and progression free survival. We then wanted to look at the IDH wild type group because they seemed to have the molecular signatures of glioblastoma, the highest grade form of astrocytoma, and in fact we saw that the lower grade gliomas that were IDH wild type had almost the exact frequency and types of mutations as IDH wild type glioblastoma, including P53, P10, EGFR amplification and CDK and 2A amplification, but also on the less frequently mutated genes, they were very similar in nature. Similarly, we found that specific oncogenic gene fusions noted in the IDH wild type lower grade gliomas were very similar to GBMs. They included the FGFR3 translocations and EGFR gene fusions that are believed to be oncogenic in similar frequency to the GBMs. So in fact, the lower grade gliomas that are IDH wild type really have the molecular findings and the outcomes of glioblastoma rather than the other lower grade gliomas that are IDH mutant. This is a very important step in the field of neuro-oncology to be sure. What does this mean for therapy? Well, the RPPA analysis did shed some light on that. There were certain proteins that were upregulated in this IDH wild type group that might be targets of therapy, and certainly tyrosine kinases like EGFR, CMAT, HER2 might be targets of therapy. So in summary, six histologic diagnoses can be distilled into three robust clinically relevant molecular classes. IDH mutant 1P19 Q-coated tumors have CIC, FUBP promoter notch, and PIC3A mutations. IDH mutant non-coated tumors are characterized by P53, ATRX, and AQ24 amplifications, and the IDH wild type lower grade gliomas really have molecular alterations and clinical behavior similar to GBM. So thank you very much for listening, and I'd like to thank the analysis working group and the leaders of it. This has really been an incredible project for me to help lead. Time for one question. One question. The question is really, what's the major difference between the IDH wild type and GBM? You know they're very similar, right? Because they're histologically a little bit different. Is it any molecular differences? Because you're saying the mutation profile is the same, everything is the same, but there must be something epigenetically different or something? Yeah. At the genomic level, copy number and mutations are virtually the same. In terms of survival, the IDH wild type LGGs may have a slightly improved survival. And then if you look at gene expression analysis, they're not the same biologically per se. I think the introduction of necrosis and hypoxia into GBMs does lead them to segregate themselves based on gene expression profiling. So, slightly different biologically but genomically identical. So I think this could be epigenomically different? Epigenomically, they look very similar. So, as TCGA generates huge amount of data, the fundamental question is how to use this genome data to guide patient care. So next week is Dr. Andrew Mado from British Columbia and cancer agents here to talk about how to use TCGA data to inform procedure medicine in late stage cancer settings.


We continue today with chapter 26. For they have come. Think but how holy you must be from whom the voice for God calls lovingly unto your brother, that you may awaken him the voice that answers to your call. Think how holy he must be when in him sleeps your own salvation with his freedom joined. However much you wish he be condemned, God is in him. And never will you know he is in you as well while you attack his chosen home and battle with his host. Regard him gently. Look with loving eyes on him who carries Christ within him, that you may behold his glory and rejoice that heaven is not separate from you. Is it too much to ask a little trust for him who carries Christ to you, that you may be forgiven all your sins and left without a single one you cherish still? Did not that a shadow held between your brother and yourself obscures the face of Christ and memory of God? And would you trade them for an ancient hate?  The blood of hatred fades to let the grass grow green again and let the flowers be all white and sparkly with the summer sun. What was a place of death has now become a living temple in a world of light because of them. It is their presence which has lifted holiness again to take its ancient place upon an ancient throne because of them have miracles sprung up as grass and flowers on the barren ground that hate had scorched and rendered desolate. What hate has wrought have they undone and now you stand on ground so holy heaven leans to join with it and make it like itself. The shadow of an ancient hate has gone and all the blight and withering have passed forever from the land where they have come. What is a hundred or a thousand years to them or tens of thousands? When they come, time's purpose is fulfilled. What never was passes to nothingness when they have come. What hatred claimed is given up to love and freedom lights up every living thing and lifts it into heaven where the lights grow even brighter as each one comes home. The incomplete is made complete again and heaven's joy has been increased because what is its own has been restored to it. The bloodied earth is cleansed and the insane have shed their garments of insanity to join them on the ground whereon you stand. Heaven is grateful for this gift of what has been withheld so long for they have come to gather in their own. What has been locked is opened. What was held apart from light is given up. That light may shine on it and leave no space nor distance lingering between the light of heaven and the world. The holiest of all the spots on earth is where an ancient hatred has become a present love and they come quickly to the living temple where a home for them has been set up. There is no place in heaven holier and they have come to dwell within the temple offered them to be their resting place as well as yours. What hatred has released to love becomes the brightest light in heaven's radiance and all the lights in heaven brighter grow in gratitude for what has been restored. Around you angels hover lovingly to keep away all darkened thoughts of sin and keep the light where it has entered in. Your footprints lighten up the world for where you walk forgiveness gladly goes with you. No one on earth but offers thanks to one who has restored his home and sheltered him from bitter winter and freezing cold. And shall the Lord of heaven and his son give less in gratitude for so much more? Now is the temple of the living God rebuilt as host again to him by whom it was created where he dwells, his son dwells with him, never separate. And they give thanks that their welcome made at last where stood across stands now the risen Christ and ancient scars are healed within his sight. An ancient miracle has come to bless and to replace an ancient enmity that came to kill. In gentle gratitude to God the Father and the Son return to what is theirs and will forever be. Now is the Holy Spirit's purpose done for they have come for they have come at last and from the workbook. Lesson 208 I am not a body I am free for I am still as God created me. The peace of God is shining in me now I will be still and let the earth be still along with me and in that stillness we will find the peace of God. It is within my heart which witnesses to God himself I am not a body I am free for I am still as God created me. Amen.


I remind members of the Covid-related measures in place and that face masks should be worn while moving around the chamber and around the Holyrood campus. The next item of business is portfolio questions and the portfolio is rural affairs and islands. As ever, if a member wishes to ask a supplementary question, could they please press their request-to-speak buttons or place an arm in the chat function during the relevant question? To ask the Scottish Government what discussions the rural affairs secretary has had with ministerial colleagues regarding cross-government action to improve connectivity for Scotland's island communities. Cabinet Secretary for Rural Affairs and Islands is my responsibility to ensure cross-government co-ordination on islands, and that involves regularly meeting with ministerial colleagues across all portfolios, including the Minister for Transport. Work continues across both rural affairs and islands and transport portfolios at ministerial and official level to ensure that a co-ordinated response to the challenges that arise for our island communities. Services, tourism and cultural events are essential to islanders, but their local economy is being put at risk by failing ferries. The message from ferry bodies in the Scottish Government is now that islanders' expertise should not even be represented in Calmax and Seamills boards. Why is the central belt-focused SNP Government ignoring islanders and what action will it take to make sure that islanders' voices are heard? I refute that claim straight off as well. This is not a central belt government. I represent a rural constituency, as do a number of my colleagues as well. That is absolutely at the heart of the work that I do as Cabinet Secretary for Rural Affairs. In relation to the point that the member has raised, we are absolutely committed to ensuring that island residents and communities' views are represented appropriately. We have asked the new chair as a priority to consider how that might be achieved. Residents of island communities are free to apply to become members of the DML board if they wish. We advertised the recent chair in three non-executive director positions widely, including on the vessels travelling to our island communities. Members were appointed based on their abilities and understanding of the role of transport, including ferries, in maintaining the economic and social integrity of the highlands and islands. That is a requirement for all board members. Transport links are integral to rural disparate communities. The island of Dura in my constituency has had a 40 per cent population increase in the past 10 years. However, their transport links need some improvement. Can the cabinet secretary outline what plans the Scottish Government has to engage with island residents regarding its connectivity needs as it progresses its commitment to deliver the island's connectivity plan and ensure that our island communities flourish? Responsibility for ferries lies with the Minister for Transport, Graham Day. As Cabinet Secretary for Rural Affairs and Islands, I absolutely understand the interdependency of transport and the wider challenges of island community sustainability. That is a message that I was happy to convey in recent engagement that I had with the ferries community board. I remain absolutely committed to ensuring that the Scottish Government will continue to engage on population challenges, including island connectivity needs through developing positive policies that we have talked about previously in the chamber, such as island spawns, the carbon neutral islands and the island's connectivity plan. The island's connectivity plan, which is going to replace the current ferries plan from January 2023, will include a long-term investment programme for new ferries and development imports that aim to improve resilience, reliability, capacity and accessibility to meet the needs of our island communities. To ask the Scottish Government what assessment it has made of the implications for the fishing sector in Scotland of the outcomes of the sea fisheries negotiations. Our aim across all negotiations is to find a balanced and sustainable outcome for the Scottish fishing industry, supporting the sector today as well as ensuring its future. I am pleased to say that the coastal states consultations have successfully concluded, and we have reached agreement on 2022 catch limits for mackerel, blue-whiting and atlantoscandi and herring and a work plan to continue sharing discussions in early 2022. We estimate the value of the deal for Scotland to be worth around £186 million through 2022. The bilateral talks are on-going and, although we do not yet have the final outcomes, I can assure the chamber that we are engaging constructively with our international fishing partners to get the best possible outcome for Scotland. I will notify the Parliament of the outcome of all the negotiations when they are complete. I thank the cabinet secretary for that response. Can the cabinet secretary confirm the change to tonnage in fish exports from Scotland and whether she flecks the promises of improvements made by proponents of Brexit per chance? We have the data that has been obtained from HMRC, and that shows for the first two quarters of this year. The fish exported to the EU from Scotland was just over 68,000 tonnes, which is a 13.5 per cent decrease from the same period in 2019. I think that that goes to show the clear evidence of the disastrous impact on the Scottish seafood sector, which relied heavily on the EU for trade. Over 70 per cent of Scottish seafood exports were to the EU in 2019, and that was worth over £778 million. The Scottish Salmon Producers organisation reported losses of at least £11 million in January alone as a direct result of the changes that were brought about by Brexit. Additionally, the organisation estimates that businesses are spending around £200,000 a month due to extra paperwork. The loss in trade to the seafood sector since early January this year offers the clearest evidence that we have seen so far of the additional costs and impact of the association with becoming an EU third country and the trade frictions that that has introduced. The cabinet secretary will want to ensure that new offshore wind developments do not interrupt established fishing operations. However, the Scottish Shetland Fishermen's Association earlier this week highlighted a number of concerns around data use, noting a lack of reflection for movements of non-Scottish vessels or consideration of issues caused to fishermen outside of bottom trawling. Will the cabinet secretary look into those concerns and if required take action to support the industry when developments are being considered? We just want to assure the member that we have regular engagement both myself and my officials with the industry, but for their particular concerns there that they wish to discuss with me, they are more than happy to look into that. Question 3, Murdo Fraser. To ask the Scottish Government what compensation will be made available to farmers who have been impacted by beaver activities. The reintroduction of beavers represents a step forward in restoring Scotland's biodiversity. We want to ensure that more of Scotland benefits from the biodiversity enhancements brought by beavers. NatureScot will continue to support land and fisheries managers experiencing negative beaver impacts through providing free advice and help with practical management through the Scottish beaver mitigation scheme. We want to work with land managers to find ways of coexisting with beaver populations. Developing support mechanisms for land managers who are delivering positive biodiversity outcomes will be more productive in the longer term than compensation based on negative impacts. Murdo Fraser. I thank the minister for that response. The Government's recent decision to allow the expansion of the beaver population into new parts of Scotland has been welcomed by some, but many farmers are already suffering significant financial loss due to beaver damage, for example, to flood banks or cutting down trees, and as a result they have lost crops. Hearing that they are going to be offered free advice is going to be of very little comfort to them when they are facing significant financial loss. What new financial assistance will be made available to farmers who are suffering as a result of the decision made by the Scottish Government? The NatureScot beaver mitigation scheme is now in place and can offer land managers advice and practical assistance with mitigating the impacts of beavers, including taking proactive measures to prevent impacts where they are appropriate. That includes the protection of high-value trees, heritage trees, landscape trees and immunity trees, the installation of flow devices in beaver dams and where appropriate beaver exclusion ffensing. NatureScot will continue to work with stakeholders in developing the mitigation scheme and with new and innovative approaches. Licensing approaches are available to prevent serious damage where mitigation cannot resolve the conflict. Supplementary first, Ariane Burgess. Scottish Environment links beaver champion. I am delighted that beavers will now be allowed to expand across the country. It means that many more people can benefit from the ecological benefits they bring as ecosystem engineers, as well as the sheer joy of once again sharing our natural environment with these charismatic animals. I am sure that there are many land managers who would benefit from and welcome beavers on their land. Can the minister advise what they need to do to receive a translocated beaver family? I thank the member very much for her question and for her work as Scottish Environment links beaver champion. There are many people in Scotland who would love to have beavers on their land if they have the appropriate environment for them, and any land managers interested in having translocated beavers on their land should contact NatureScot through the email and phone contact details on their webpage beaversatnature.scot or<PHONE_NUMBER>4. NatureScot will discuss the different aspects of translocating beavers to their site, and that includes scoping site suitability, carrying out effective public engagement and consultation, the practical aspects of trapping and transportation, captive holding, whilst animals are being health screened and some level of post release monitoring. There is no doubt that I have to agree that there are definitely farmers not far from our constituency, Mr Fraser, who have actually looked to get beavers translocated to their land. However, I accept that they make an important contribution to restoring Scotland's natural environment, but their modifications to the environment can in some places produce negative impacts on agricultural land. Can the cabinet secretary provide any further information as to what the support from the Scottish Government will be, particularly to the fences that you just alluded to a minute ago, to help to negate the negative impacts of beavers being introduced? Absolutely. I thank the member for the question. The member is quite correct that the reintroduction of beavers demonstrates our commitment to protect and enhance biodiversity. Beavers have a really positive impact on biodiversity, creating new wetland habitat for a range of important species. They also help to reduce flood risk by slowing down flows and improving water quality by filtering out sediments. The NatureScot beaver mitigation scheme is in place and can offer land managers practical assistance in mitigating the impact, such as what I have listed already, protecting high-value trees, installation of flow devices and where appropriate beaver exclusion ffensing. NatureScot will continue to work with stakeholders in developing those mitigation schemes and making sure that land owners and land managers can live alongside beavers. To ask the Scottish Government whether it will provide an update on its work outlined in the programme for government to determine how best to support new and young entrants into farming. We are continuing to work on options that will best add to the existing support provided by our farming opportunities for new entrants, the Scottish Land Matching Service, Farm Advisory Service and direct payments. Our aim remains that the outcome will be announced soon. Despite the SNP throwing taxpayer money and encouraging young women to join agricultural industry, the young farmers start-up grant scheme only handed out funding to 62 women across Scotland over four years. In 2019, there was only one successful applicant, just one. Young farmers deserve better than this, especially young women in rural areas. Does the cabinet secretary believe that a single successful applicant in one year is an acceptable return of investment? Will she outline exactly what measures she is taking to improve on this abysmal record? We are absolutely committed to ensuring that we see more new entrants into farming. I was just visiting an opportunity this week through the farming opportunities for new entrants where we have been looking at the opportunities that can be offered through public land to encourage new entrants as well. However, in terms of getting more women and more diversity into agriculture, absolutely, can we do more? I agree with that, too. That is why we made specific commitments in our manifesto in relation to getting more women into agriculture and to make Scottish agriculture more inclusive. Women living and working in Scottish agriculture are an essential part of the future of the rural economy. Developing and expanding their skills will ensure the long-term sustainability of the agricultural sector. We have committed £300,000 this financial year to bringing about practical solutions to support women. That includes the wider role of being your best self, personal development training, a pilot of agricultural business skills training and a project to test innovative solutions for childcare in rural areas. The development of Skillshare, which is a skill sharing app to encourage greater participation in rural and land-based learning. During the course of this session of Parliament, we will double the funding to support women in agriculture of up to £600,000. To ask the Scottish Government what measures are being put in place to protect livestock against avian flu. The chief veterinary officer for Scotland confirmed a second outbreak of highly pathogenic avian influenza H5N1 at premises near Gretna in Dumfries and Galloway on 3 December. As with the earlier case in Angus, protection and surveillance zones were immediately established around the infected premises, which put in place a range of mandatory disease control measures, such as restrictions on the movement of poultry, domestic mammals, carcasses, eggs and other poultry products. In response to an increased risk of spread of disease from migratory birds, Scottish ministers introduced a national avian influenza prevention zone on 3 November. Those measures were enhanced on 29 November, requiring housing of all poultry and captive birds and for our keepers to apply further robust biosecurity measures, which are key to preventing the spread of disease. Advice from public health and from epidemiologists is that this strain of highly pathogenic avian influenza represents a very low risk to human health and to other mammals. Investigations as to the source of the infection and links with the current infected premises in livestock is an element of the wider epidemiological investigation process to ensure that the disease is stamped out as quickly as possible. I thank the cabinet secretary for that answer. With Covid-19 impacting businesses in various ways, what support is in place for bird keepers if the worst should happen to their livestock? Compensation is paid for birds that have to be called as part of our disease response in line with valuation tables. Those are updated regularly by the animal and plant health agency on behalf of the Scottish Government. The Scottish Government does not compensate for any dead or visibly ill birds, which is in line with the policy elsewhere in the UK. If a larger poultry or captive bird business is in a disease control zone, there might be trade issues that are associated with regionalisation, and the Scottish Government works with the deffra market access and veterinary trade facilitation teams who liaise with poultry industry stakeholders and the APHA Centre for international trade to really try and understand and address the issues and priorities for poultry exports. Deffra market access also liaise with British Embassy colleagues globally to answer trade-related queries from central competent authorities and provide updates to third country trading partners and really seek clarity on the terms of trade where that is required. The Scottish Government will liaise with the National Farmers Union Scotland and the British Industry Council to support those affected businesses where that is required. However, I emphasise again that it is good by security that is extremely important for keepers to practice in order to reduce the risk of their birds being infected by a notifiable avian disease. To ask the Scottish Government what action it is taking to support the agriculture and fisheries sectors to play a leading role in delivering a net zero Scotland. The Scottish Government is committed to driving action to support all sectors to transition to net zero and ensuring that that transition is a just one. It is clear that agriculture and fisheries have to play a leading role in that. Policies and programmes such as the blue economy vision, the future fisheries management strategy and the agriculture reform implementation oversight board, as well as practical measures like the Marine Fund Scotland and the national test programme are enabling conversations and supporting innovation, as well as helping farmers and fishers understand how their work impacts on climate and nature and what they can do to deliver that change. Given that the Greens and SNP partnership agreement has got nothing to say about ending overfishing, it has got nothing to say about ending the wasteful practice of discarding, and it has got nothing to say about landing a bigger share of the catch closer to home in Scottish ports, does the cabinet secretary think that that is consistent with securing sustainability and environmental recovery for our inshore seas and fisheries? The co-operation agreement that we have with the Scottish Green Party is highly ambitious in a lot of areas and is really looking to protect our marine environment. Just because certain specific elements are not listed within that, that does not mean that they are of course not important. I would also outline the measures that we have that we have already set out as part of our future fisheries management strategy about how we intend to take the steps to make sure that our marine environment is a healthy one and that we continue to fish in a sustainable way. There are plans to locate offshore wind farms and rich fishing grounds west of Shetland to provide electricity for oil and gas installations. Shetland Fisherman's Association has highlighted flaws in the offshore wind farm consultation process, as Jamie Halcro Johnston has already alluded to earlier. Can the Scottish Government commit to gathering and fully assessing data on all fishing activity from those areas and listen to local fishermen's concerns? I would just emphasise and reiterate what I stated in my previous response to Jamie Halcro Johnston. I am the fisheries minister and it is critical for me that I listen to the concerns that are voiced and I am more than happy to engage on that and to listen to the Shetland Fisherman's Association concerns directly. In light of our climate emergency and the urgent need to give farmers communities and landowners clarity over net zero, will the Scottish Government join Scottish Conservatives calls back by the Woodland Trust Scotland to simplify and make changes to grant schemes for riparian planting? Now, rather than waiting until 2024 when it might just be too late to save iconic species such as salmon. There are a number of pieces that are obviously on-going in relation to protecting our iconic species, as Rachel Hamilton mentioned, and I would be more than happy to contact her about the suggestion that she has there about riparian tree planting and how that will be considered in the future. Can the minister provide any further details as to the steps that are being taken to introduce ammonia emissions in the agricultural sector? In response to the member, we have updated the regulations that govern controls on the storage and application of slurry and digested by introducing the legislative requirement to use low-emission precision spreading equipment. We can reduce the ammonia emissions from agriculture by up to 70 per cent, which ultimately protects our vital water environment and reduces the agricultural impact on climate change. That will also make an important contribution to the commitments on reducing air pollutant emissions from agricultural activity, which are set out in our new air quality strategy, which was published earlier this year. Through the national test programme, which I announced at the end of October, we will also be providing support for nutrient management plans, supporting more efficient fertiliser use and so reduce ammonia emissions. Thank you very much indeed, cabinet secretary. That concludes portfolio questions with a slight pause while the front bench is changing and we move on to the next item of business.


Hey sports fans, I'm Jameson Fleming in the At Week Visual Newsroom, and we're checking out the first MLB live stream exclusively on Facebook Watch. I'm going to find out how it's going. So what do you guys think so far of the experience? The baseball experience itself is good. There are some complications. It's not as technically sound, so it looks like in my humble baseball opinion, Facebook has some technical kinks to work out. Like what? Like one, this box is massive. Usually on a standard broadcast, I would be much smaller and take up less room. There have been tons of complaints in the comments about that. So that's just like one big thing that they haven't changed, and it's now the fourth inning. So next time around. I think it's cool that we could watch a baseball game on Facebook, and there are no commercials, which is good for the fan. And I like that they are reading the comments, they are displaying the comments during the broadcast, and they're even tweaking their graphics as they go along. When the game started, they had this huge graphic that took up most of the screen, and now you have the box that's too big like John said, but they are tweaking it as the game goes along. What do you think about all these emojis floating up and down the screen as the game goes on? I find them annoying. So with you John, you've worked here at Adweek for a number of years, you've got lots of co-workers that you're Facebook friends with. How do you feel about them knowing that you're currently watching this game? One actually just signed on. What is going on? I don't mind that my co-workers and friends realize that I'm watching the game, because I'm probably going to be watching it anyway, especially if it's for free on Facebook. But given the recent issues with Facebook and their privacy, I can understand how it would be a little unnerving to some people who are a little bit more protective. I'm just of the cam that Facebook has messed up once, and they're probably working on fixing that and fine-tuning all of their privacy issues. So to me it's not that big of a deal, but to other people who are more concerned about the privacy issues could be a really big deal. There you have it, mixed reviews from sports fans on the first exclusive live stream of a Major League Baseball game on Facebook Watch. For Adweek, I'm James Simplenny.


Pounds is a form of money used in England Here in America, we use the dollar In England, they do not use the dollar rather they use pounds