What up what up? What's going down? It's going down with y'all What's happening man? What's happening there, buddy waiting for some more people to join in going down? Yo, what's good? What's good? Appreciate all y'all joining in on short notice. I wanted to stream the night want to Get a little streaming session in cuz Man lately I've been having some pretty good game plays if you follow me on Instagram You'll be seeing the scores. I'll be getting man. So I want to try to capture that on the stream I'm gonna show you guys the setup. I've been using But yeah, man, I'm gonna want to just have a good time on here and be playing solo So I won't be playing with any of the subscribers not today. I have to do that sometime later on this week potentially Yeah, my ears still kind of messed up Might have to probably go to the doctor soon So what's going down everybody? But let me show y'all the class now. I have been using the cx9 It's a nice build but I ain't gonna lie to you this class set up. It's like a nice Definitely even though I believe this was a gun that was supposed to come out a while ago But if they never dropped it until recently, but yeah, I gotta go nice low recoil Definitely I see a lot of people using this like it rips from medium to close range. It shreds, but Lately I've been getting my high kill gameplays with the good old trusty and faithful Bison, bro. I have been destroying people With this right been destroying people with this gun Y'all have the monolithic suppressor This is the barrel I'm using tac laser gi mini and a stipple grip tape man. I have literally been shredding people With this gun no fire shotgun, bro. I'm not trolling. I'm literally just gonna be playing the game Just on some chill stuff trying to get the highest kills as possible That's the objective of this stream To consecutively get multiple high kill gameplays and potentially get the nuke So i'm gonna try to So I gotta make sure everything's set up on here. Let me know if there's any visual delay so I can fix it if there is What you talking about logan? I'm feeling all right, man I'm feeling all right All the rock the dude named ross Yeah Using the last stream. Do you have any Jordan 11s? Yes, I do I have the jubilees Oh, I played against the succulents guy. I don't know how many times I played against this guy This lobby Oh, we got it. Is there any delay y'all? I'm off right now. Yo What's only Y'all hear them in the chat. No delay. Is there any delay? My Sunday's been pretty good. Yeah, there's only one person. That's not fun. They backed out person left No, no delay on my and good. I just want to make sure there's no delay Well, this was a goodness a waste and easy dub freaking freaking freak get away from me. Oh my god What's going down, man? I don't know.  This is really boring I honestly didn't want to back out, but it's a free win.  There's only one person He is clapping them though. He's clapping their cheeks.  What is wrong with this Taliban dude? Is he all right? Is he okay? Y'all hear this Right bro, what the fuck's wrong with this Taliban, dude? Dude is tripping, bro. Y'all hear this right?  Y'all hear this you got died.  Are you good? Bro, what is wrong? Why does he keep saying that?   Wait, what?   Oh, he got him, bro. He's tripping, bro Well, that's game.  No idea what he was God and we are still lost Oh, man That wish that was better Caught in a nutshell facts Yep Bro His a do not come into my chat asking me to dance like i'm Some goddamn monkey, bro. Like I don't just be dancing all the time, bro You will get blocked don't come into my chat asking me just dance Like i'm About to shuck and jive for you. Like what are you doing, bro? It's not the first time i've seen people come into your to my chat and be like dance I'm not about to dance, bro.  You don't even be like, yo, it was good raw. She'd be like dance No, you dance You dance jt 201 you you bust a move for us, man and send it to me on instagram.  Nope. I have not popped my ear yet. Unfortunately Unfortunately, my ear is still kind of muffled my left ear So i'm probably have to go to the doctors at some point soon And try to get that fixed because I ain't really much I can do honestly What's Ross? What's up with man flirting you? What you talking about you talking about old dude? Man, I like your content. Thank you chief tv ross any good movies lately Uh, I saw the uh suicide squad I enjoyed that movie I actually did the recent one. It's better than the first one in my opinion my humble opinion. I think it's better than the first one ross any uh What's good ross just dropped dropped a nuke on bo4 feeling feeling good. Hey man I'm play bo4 and so long bruh Like it has been so long since I played blackouts for bro Could be an ear infection But that's the thing. I'm not sure if it is. I think it has something to do with uh Once I got off the flight last week or a week before last Yeah, well now it was last week once I got off the flight my ear Ear was definitely popping Like crazy You played need for speed. Uh, I haven't on uh I haven't on the switch.  I don't even know why I bought that game I bought that game and I don't even play it anymore Granted, I really only have my nintendo switch just for like, you know, when people come over Yeah, I mean It's not much they can do or they let me rephrase that there is a lot they can do with raw It's just they won't They're not gonna fix up raw.  Nope. 2k. I've stopped playing sports games a long time ago, bro I don't play no sports games Thanks Ross for telling me I look good I prefer modern warfare But I will be getting battlefield should be coming out in a couple months and I will be playing the new halo so Yeah, I don't know if I'm gonna be getting a new call of duty.   This is a god tier lobby. I love it I'm gonna call this in right here. It's chopper time, man Let's go. He's still in there though. Bro. He's still in there. All right, we're out to say Turn the feed up. Yeah, I'm trying to go off man.   No Damn it. He got me. Uh What's going down there everybody? I'm muted No, I'm not All right. Oh, they they're right above. Yeah, that dude is not having a good time right now. Oh, did y'all see that? I had a feeling someone was already in there I'm gonna let them cap it Try to hold these angles up here. Yeah, yeah, they got it down there. Oh, okay Yep, I'm getting out of here. Oh my god. That was close. He almost got me. I'm not the flamin Oh my god, dog I'm telling y'all if you haven't used the bison yet Definitely use it Oh, man, we're holding it down right now. I gotta move my mic up. There you go. You got him still I got a pretty good solid team Trying to get as many kills as possible, but I actually have a solid team Come on Oh, I thought that was an enemy go this way He was about to set up shop.  Yep? Could be coming up. Yep. I had a feeling Oh, yeah, they're downstairs. I don't want to risk going down there yet About him and his flamin shot guy Is he gonna come up here? Well, that was game. I tried to get as many kills, but Well, you know, bro, you need to use a fire shotgun.   You hear it.  Look at the lobby. I was going against lord have mercy Were they awful? But I don't get those lobbies all the time. Maybe I can get into this one I really gotta focus in That's the only way so if I if I don't talk is because I'm focusing trying to get the highest kills It's possible Appreciate you ross is so weird saying that Ross is talking to Ross Who shops at Ross needed that water Yeah, rogue nation is not engaged right now Just hope we don't cross lobbies. It won't happen.  I'm just playing forever. I don't know I'm not a scrub on this game. I'm all right.    Oh my Come on. I'm dead Y'all see how long I get stunned Both of them are in there. All they have to do is literally go around.  Damn it. Damn it. All right Road to cck Uh, probably won't be streaming with stuff tomorrow.  It's just There's a good chance. I may I may not I I'll let you know See the thumbnail up. You know, I'm streaming. What's up? Are they gonna spawn here?  Oh, I'm about to call her back Hey, I'm live streaming right now. Let me get you back. All right Got distracted for a quick second Geez Is everyone dead?  I don't know why we don't have a full team yet I knew he was right there. I don't know if they're gonna spawn. They're probably spawned back over here Most likely He got us both God damn it, bro Day is going pretty good. Can't complain Granny my team ain't helping me out most look at my team. Yo, look at my team. I'm on teammates Jesus Bro, I'm trying y'all I'm trying y'all. I'm giving it my best shot. Y'all see me trying Y'all see me trying Y'all see me trying, bro We're getting clapped. None of my teammates know that he's right there. None of them Bro, he's right there. Jesus.  No, he went this way Oh my god teammates Just push Jesus. Oh, it's ours about as useful.  Oh my god Hold down this left side. I'm watching you. I'm watching. I got your back. No No Now I spawn here. Why did I spawn there?  I got the triple.   I'm cooking up like a hot pocket. Oh, I'm dead.  I still killed him.  I tried I tried man. No, I actually have not met a wrestler before I went 46 and 29 and Look at my I want y'all look at my team.   But yeah, no, they didn't leave They were still in the game the entire time, bro.  Yes, but I do not play because I don't like cold war. I think cold war is a It is a step down from modern warfare Uh, graphically, it's a step down. I don't like the extreme skill based matchmaking This you can tell the skill based matchmaking is not that strong in this game because look at who I was playing with A whole bunch of bots on my team in the previous game. I was going against a whole bunch of bots So it's like the skill based matchmaking is not as strong In this game as it is in cold war I had cold war. I still have cold war. I just have I'm not installing on there It will not grace my my next gen console.  I'm not no, but I'm not hopping on cold war, bro. No all right Nope, nope.  I don't even play it anymore All right, we got into a game that's just starting up. All right, cool But now warzone is definitely dying because of all the hackers, bro My night's going pretty good. I'm shopping it up with you guys. So It's going pretty good. I think I played with it's over whoever's the guy that's on our team. That's pretty good Yeah, it's over guy. He's actually pretty good.  Don't tell me he's looking at my body, bro Because he got me behind taxi. He will get embarrassed Yo, I think they I swear I think they Oh my god, uh, I wouldn't I don't really care to play the the next Title because it's going to be another war war two title.  Well, yeah, you know What someone killed him good I Should be spawning this way coming this way He got him Yeah, what see you see how good my team is now Look how good my team is and I played against it's over. Look how good my team is Look at this Look how good they are Now I was literally just in a lobby full of bots Teammates and now I have competent teammates He's going off with this Push destroyer holding it down Oh Dub is actually getting better at Call of Duty He actually is getting better at Call of Duty Granite that one, you know, he usually doesn't play it, but he's getting a lot better at it. So I don't know what you're doing Y'all see how people play He's just sitting there. Bro. He's literally just sitting in the back Move out the way.      It's over. This is done See, like I said, the skill base matchmaking in this game just doesn't make sense Like it's it's really not there to be honest with you as much.   So you really don't have to reload too often. That's why I like running this gun Okay, this is going to be a sweat lobby because I've played against the murder clan before Mr. Beans This is going to be a sweat lobby, bro This is going to be a sweat lobby Are we are they on my team? Nope Murder.   He held Let's see if I can get him off the spawn Yeah, that yeah, I played against these guys earlier today Yep He thinks I'm on pc He thinks I'm on pc. I'm on console my guy Bro, he just spawned there. Oh, he's shooting my dead body to disrespect He's shooting my dead body. He's frustrated He's mad. Yep. They're gonna start spawning here. Yep.  He's so mad You know someone's frustrating when they start shooting your dead body No, bro. Oh, I didn't know where he was Still get me house way Then probably not gonna spawn over here. Yep. They spawned over there. Yeah, I'm not gonna challenge that We're over here There's not much I can do like I'm really clapping them As y'all can see I'm really clapping them But it's just me that's doing it. Look at me and one other person And they just keep doing it.  There's someone over here Yep Get it out of here. Get it out.  I tried y'all Really push them.     What could I do? Look at my team I gave you my best shot, man I appreciate you chiseled, man. I'm gonna give Ross F Give Ross my brother out here And send To the to the shout out around man. Hey, I was trying my best, man I was trying my best Oh in the same lobby Mike Ross who? I don't mind playing against some sweats As long as I have a decent team now my team back out. I'm not doing it Granny I haven't really had no decent team lately Hey prayers for Haiti, man Definitely prayers for Haiti That is crazy That magnitude of an earthquake man. Like that's that's something scary, but not gonna lie to you Very scary Some prayers go out to the the families and their loved ones Oh, he's talking about the uh the Ross in the chat. Oh, okay. I know what he was talking about. All right Let's see. Oh, how Did they not spawn in I don't think they spawned in They forfeit it. Wow They forfeit it bro Why They legit Oh, wow, they forfeit it. They backed out. I think they backed out Yeah, I think they backed out, bro No, y'all still in the lobby where Are you playing Raymond wait, what's going on here? I don't know what's going on here.  Oh, I guess we head to the new HQ.  I'm out What's going down? Is this the real dub Anisha, man? Is this the real dub? Or is this the fake dub? I don't know, man No, this is the fake dub because I just talked to dub earlier today. So he definitely would not say that Yeah, that's not the real you're not the real dub No, it's not bro. It's not the real one, bro. They both died Oh my god, I don't know what my other teammate is doing. I'm dead.    Hey buddy. All right. Thank you. He doesn't know Right there There's one more He didn't know thank you teammate. Thank you for helping me That's not the real dub Anisha. That's what I'm saying. That's not that's not really dub. That's somebody else They just using his picture That's literally all it is. That's not the real dub I want to figure out why there's only two of us That was all hip-fire by the way.  Yo, he's in here. Yep. He went up top.  I'm trying y'all It's only three of us against almost a full team How was that fair call of duty? It's not Why won't they give us extra teammates?  Is this cold war? Do you not know call of duties? What does this look like cold war? Bro, this is modern warfare. He said is this cold war? What It's been only three people this entire time. I want y'all to understand. It's only been three people I ain't know who to shoot. I knew people was gonna try to hit us with this link. That's what I would do Bro, look at this. Look at my score. Look at this.  I dropped 50 kills Granny, I didn't get more teammates until later on. So I don't even None of them are even to save on it Bro, look at my team, bro. What are they doing? What are they actually doing? Nothing. Oh, okay. I just wanted to make sure Uh dropped 54 kills still nothing. Thank you teammate. You actually helped me I tried y'all I tried y'all. I dropped the 60 bomb. Oh, we got it. Look at that. We actually got it Wow Look at the score y'all We actually got it ladies and gents Still got blown out bro. I dropped 60 I did all I could do Is he gonna peek? No. Yeah, he would have died too. I dropped 67. Yo, I tried I went dropped 67 kills almost dropped the 70 bomb I went 67 and 16 We didn't even have a team down here towards the end of the day 67 and 16, bro It's only so much I can do one game. I get good teams next game. I don't it's it's just back and forth back and forth Oh, man, bro. It's kind of frustrating. What's going down phase? I'll be trying y'all Let's see if I can get that elusive 70 gameplay 70 kill gameplay I don't know why you keep asking who do I like wwe like I I get what you're doing the Spelling w the way dub name and smell I get it But yes, obviously I enjoy wwe. I just wish it was better. I don't hate it Just wish it was better It just wouldn't make sense I get it because you know She's in a relationship I'm not sure which one it is. I think it's I'm not sure but Wow, they they got a good where it's at They have a good where it's at I'm so mad at my teammate. He did nothing. He sat there.   I was surrounded We may actually tap it. Oh, there we go. Yeah, he got me Yeah, this dude he's running Looks like uh, yeah the losing He's actually pretty good It's gonna be in this building right here Well, I think she is correct. What's the other guy? You got something Hmm. She know her stuff. Oh, he's right here. I think yeah, she know her stuff I'm not gonna call it in yet. I'm gonna call it out. Yeah, that's how I'm pushing back He's looking for me. He said how Trying to call it in y'all Trying to hold it down y'all. That's what you call good play You're not getting out of this one ladies and gentlemen y'all not getting this point Even if you wanted to get this point you couldn't get this point Yeah, baby. Yeah You're not getting this Need me more kills Here you go on that way Bro, this whoever this chick is she knows where the points are gonna be at So she plays this just as much as me probably should What are you doing here, bro? Oh, I want your gun. What is this y'all doing? I'm almost out of ammo. I didn't know I was that close Well, I got some more ammo y'all. You actually have some good teammates. I actually have some solid I'm not going that way She's coming this way. That was crazy.  It was determined right here now Come on peek it. Oh my god. It's the flaming shotguns Bro, this dude He went from the uzi to the flaming shotgun Get off me bro. They got hit with the shaking bake I call that the shaking bake special, man It was delicious. I'm dead. I just had good teammates pretty good. He's almost about to drop the 70 bomb No, I'm trying to get this 70 bomb. Yo, what's good true billy brand? It's going down, bro What's going down, bro?  Oh Oh, you got me. I hit the 70 bomb though 73 kills ladies and gents 73 kills, bro Right here. This this this was the shaking bake move. It didn't show all of it They didn't show all of the shaking bake 73 and 22 73 and 22 man Can I go for the 80 kill?  We're trying to keep our channel in good standings We may be able to do that on patreon That'd be something we could possibly do on patreon, but when it comes to the main channel, we have to you know Play it as safe as possible.  All right, man. I'ma try to bro I've done it. I think I did it earlier. I think yesterday I dropped a hundred hundred and 22 I'ma try to man You sure that wasn't a bot lobby. That wasn't a bot lobby, bro One of those guys on the team he was smacking me at first and then I just started going off. So No, it wasn't a bot lobby. I just had good teammates And I was hitting my shots. That definitely wasn't a bot lobby. I'm telling you that now I know what a bot lobby is. Uh my favorite tech My favorite game mode used to be free for all but the past few games it has been unbearable That used to be my favorite game mode Oh, all right three v four That used to be my favorite game free for all And then domination at one point And now it's headquarters for modern warfare. I got the wrong gun out There we go. I'm dead Ross is fat. Hey moderators.   This dude literally made a whole profile with my picture. It's gonna be Ross is fat Thanks, bro. I don't know why I didn't see that guy I got all my streaks, but I'm kind of saving it. I'm gonna save it for Another one. Where's my other teammate? Bro, I am off in this Bar he is getting Getting out played Looking for me Oh, I'm trying man, bro. How are they already in there?  They didn't even cap it y'all. Oh, I got another chopper gunner. Oh, why am I right here? Please tell me y'all gonna get in there All right, dammit, bro Just get in there y'all That's all you got to do. Thank you. They want me dead y'all. Sina's not beating Roman, bro I'm sorry to tell you this I don't think this is the best stream ever, but this is the south stream. Thank you, man But yeah Sina's not winning man. I know you may want Sina to win But he's not winning bro. I just want you to know this Oh my god My teammate wouldn't even shooting at him. You just running at him. I'm gonna run. I'm gonna run at him. Let me at him. Let me at him I knew someone was over here Damn, this is actually kind of a close game Now Sina was enjoyable in Suicide Squad.  I thank you so much It's a very close game still 50 bomb already had Oh, he's already in the next spot. Yeah, I was one shot Damn, I spawned so far back Yeah, I think they got the spawns on this one. Yes. Thank god you missed I don't know what that guy was doing, but Yeah, he was just waiting in the back I'm legitimately not sure where my teammates are doing. It's it's already disabled Huh Everything's going pretty solid so far. I wish I could hear better on my left ear in my left ear, but it's cool You do what you do. Oh No, I noticed it too late. I literally spawned right next to it. This is why I run blind out y'all For this very reason.  He was so mad He was following me.  I actually want to win this game. Let's back this way He got me. Oh, he's de-backing me Damn, man, bro. It doesn't matter, bro Where's my team where's my team come on? I really want to beat these guys He not see me off me, bro Shouldn't just be sitting there doing that. Oh, you got me Damn, man.   He had 30 kills. I dropped 80 on your head.  What are we doing? I literally dropped 80 on your head and y'all barely won The only person that was holding them now was faded dinosaur.  Oh, we back in it back in it again Oh, I gotta talk to this faded dinosaur, man. Hold on.  Yeah, he's not saying nothing. So We'll see If faded could hold it down for his team again. Oh, we may be on the same team I think we're oh, we're on the same team.  We're on the same team Okay My bad y'all my mic was muted the entire time My bad. I didn't mean to have the mic muted Oh, yeah, I was locked in because we have a competition right now between me and faded who can get the most skills Keep camping up there top This is literally nothing they could do Y'all still have time to facing y'all bits.  Oh my god, bro That was fun Ah, this is how you have fun and call the duty man. He was a cool guy He was mad man I hey when it comes to getting kills especially in this game mode If I have a competent team or a decent team, I can get some kills I can go off They all backed out That was a party too.  I'm okay with that 2.24. I haven't used the groin so long, bro I got a 1.1 win loss. Y'all can't see it, but it's a 1.1 win loss Yeah, total time played 22 days One hour and 19 minutes 3,935 games played Oh, yes, I will be playing the new battlefield aguilar, bro I'm getting the game It's gonna be great, bro I cannot wait to stream and we're gonna be squadding up So if you want to squad up with your boy, I will be doing that man. It's gonna be fun Ross wait.  Thank you.  Oh, I was trying to find somewhere to go. Oh, he's going in with the 725 This should be a good lobby. No, I've never played counter strike You would think I would but no, I just never played it Is my teammates like I got thank you teammates.    I don't know what this guy is doing, bro There's one more left Oh, damn it. Damn it.   I don't know what I wasn't paying attention. I guess I could have sworn There was no one there.  Oh, I thought I could do it twice No, you cannot Oh, yeah, you can Oh, he knew I was coming on the phone.  Yep. I knew it. There we go.  I didn't know there was two of them. I gotta hit my shots. I'm not hitting my shots I'm stinking up the joint under the garage Drop another 60 bomb, man Please don't spawn behind me. Damn it. There was one person AFK Yeah, they spawned there. This is not good If we don't kill them off the spawn I tried man.  Damn It was that bad? That's when you leave the game, man. Just leave the game. Keep your sending Ross you and dub and true ability should play a scary video game on the channel. That would be funny I think that would be pretty cool Carried the team on your back. That's usually what happens not all the time, but That usually does happen. I usually end up carrying the team I'm okay with it though. I wish barely could put me in the hills gate really Ross, what's a uh paper view you wish they were bringing back? Um The first thing that comes to mind is uh armageddon. I think armageddon was a pretty cool paper view That's when you had like the the crazy matches. It was at least one stipulation match That was on that paper view and there's usually Intense I like the stage set. It was cool back then Unforgiven is a good one too Which better which is better helenicell or tlc helenicell if the storylines are good TLC usually for the most part delivers, but helenicell is always a go Bad bluff. Every view was a good one too. He's just sitting up top Hey, yo He's literally just sitting up top Thank you teammate I think the spawns flip. Yep, they did flip Coming this way Oh, no, I saw him too late. I literally saw him too late Yeah, I was not paying attention. I was listening. There's some other people in that building saw him too late Granted, it's not fair. They only have three people two of them in a clan Like that's really not fair. They don't have the advantage here. They don't even have a closer advantage Like that's not fair to them, bro. Why they won't He should have probably say that century, but it's only all right now.    Oh, no Wait, what is it? Oh, he's cheating I think he's cheating, bro. There's red dude. I think he's cheating. We're about to find out Hold on Let's see. Let's see y'all. Let's see chat No, okay. No. All right. He's not cheating He's just good I'm not gonna worry about him. He's actually just good. He's still over here Bro, he kids me every time Modded controller. You think so?  I finally killed him Yeah, he's not hacking I don't know man. I don't think he is I think he'd be doing a lot better if he was Wait, I wasn't trying to close the goddamn door. I don't know, bro. Let me see this Because yes, the cx9 is nice I don't know. I just don't think he's cheating, but I don't know Yeah, like right there. I knew where he was Yeah, I just don't think he's I'm not sure it's it's kind of a I have no idea granted. We're getting clapped right now I'm trying but we didn't clap right now Come on chase me Chase me Maybe maybe he's just that good too. He can be just that good too There are people that are just that good like he's dying for sure I just think he may just be just that good, bro I killed him while blind. Oh My god now people would think I was hacking on that one. I killed him while blind He is clapping us right now I tried but dude was good Dude was hella good. He came in to help the 18 because remember we had a full team and everybody started backing out So that wasn't my best. That's probably the worst game I've had today actually That gun does milk I just needed to make sure if he wasn't cheating or not, but I don't think he was he went 34 and 11 So I don't if he was cheating he probably would have went off even more. So it's cool though Can't win them all ladies and gents Can not win it all though That was a crazy one though Hey, Ross. Could you guys react to some new pop smoke album? Uh, I'm not sure if we will to be honest with you. We've heard That album is not that good, bro. I'm gonna be honest. It's like a few tracks that are good His actual his last album that he was you know trying to finish up When he passed away I think that was 2020 Last year was much better. All right. So we're playing against this guy again I may have to pull out the cx So he's playing controller on pc I think that's literally what it is Okay, he's playing controller on pc.  I mean granted. It's it's not a hard gun to manage but Yep, he's on 18 I really don't want to play a 3v3 on this map. Hopefully more people join I don't want to play it three. Look at this 3v4. I don't know if I want to play this, bro. I'll be honest with you I don't I don't know if I want to do this Yep, how he pulled out the the m4 Oh my god, I don't know if I want to play this bro. Well, there's more people in here We can see Wait, what? Huh? What what is he doing?  Don't get on it. Get on it. Get on it. Get on it. Get on it None of my teammates got on it No, I didn't know where yeah, he's just a solid player Oh, this gun it just rips.  Ah, I got 16 kills.  I was surrounded In the window yep, they're right behind me We're going this spot down. Oh that red dude backed out. I don't know why he backed out He definitely did back out y'all. He's not in the game no more on their team He was carrying their team.  Oh, he's sniping He's in the very very back. Oh my god.  I didn't know exactly where he was. I did not know exactly where he was.  I'm willing to bet money. He's still behind. Oh, no, they killed him Thank you. I'm about to say it's one person. I appreciate you checking out the stream for the first time Hope you're enjoying it Yeah, they get they got body right there. They got body right there He got body right there.   You got got any good games in? Oh, yeah I had a plenty of plenty of plenty of good games in this session. Yo I think this may be the last one for me tonight. I like his aggressive play. He's about to get clapped most likely Yep, and there it is. He's dead as soon as he ran in He was dead as soon as he ran in. Oh, he almost got And I got being from where though Yeah I'm using the cx nine, bro. This is the newest gun in modern warfare. It rips 65 kills man 65 kills Good way to end off the stream All easy fees 65 and 26 I'll take it I will take it man But yeah, that's it for tonight, man I hope you guys enjoyed the stream I had a good time. This was fun. Thank you so much um I will be uh recording some more content dropping in on here So be on a lookout for that this week. This is also my birthday week So I'll probably try to do a stream sometime later on this week So, uh, but thank you guys so much. I love you guys road to 60k


Hello, and welcome to This Week in the City. Highlights brought to you from the Public and Media Relations Department. I'm your host, Alicia Yeatsy. On Tuesday, August 14th, the City Council Economic Development Subcommittee will meet at 9 a.m. at City Hall. For agenda items, visit our website at columnsc.net. At 10 a.m., the Board of Zoning Appeals will meet at City Hall in Chambers. For agenda items, check out our website at columnsc.net. At 2 p.m., City Council will hold a work session, and at 6 p.m., they will hold their regular evening meeting. Again for agenda items, visit our website at columnsc.net. Now as we fast forward to Friday, it's Food Truck Friday at Columne Police Department Headquarters, located at number one, Justice Square. Food Truck Friday will start at 11.30 a.m. and end at 2 p.m. There will be various vendors who will be selling delicious food from their trucks. And on Saturday, come out for Clear the Shelter, which is an annual national event that our Columbia Animal Shelter participates in. Their goal is to clear the shelter that day, and they will be giving away for free all pets.  Also, that evening, join us at Earl Wood Park for a back to school movie. Parts of Recreation will be featuring Black Panther. The movie will begin at 7 p.m. For details regarding any of these events, call Public Relations at 545-3020, visit us on our website at columnsc.net, and follow us on our social media platforms on Facebook, Twitter, and Instagram.


Hi, I'm Dr. Gene Preuss. In this lecture, we'll look at manifest destiny, the war between the United States and Mexico, and how all of this contributed to the lingering question of slavery in the United States. We're going to first address the question, what is manifest destiny? Then how did the desire to expand west or drive the U.S. into a war with Mexico? And then finally, what was the effect of acquiring all this new land in the west and its effect on the lingering question of slavery? The term manifest destiny really comes out of the 1845 essay written by journalist John L. O'Sullivan. And it's kind of a concept that we've applied in more recent times to what was going on at that time. This spirit that the United States need to stretch from sea to sea, from the Atlantic to the Pacific, and that we needed to secure that land between those two oceans for ourselves. And what John L. O'Sullivan was writing was on Texas. That was the title of the essay, and it was about the annexation issue. And he was trying to get his fellow readers, his fellow Americans, and those who read his newspaper, to approve of annexation of Texas. And so what he said was, quote, our manifest destiny is to overspread the continent allocated by Providence for the free development of our yearly multiplying millions. In essence, what he was saying was that he saw the west as a pressure valve, and he saw Texas as the beginning of that pressure valve, and that God himself, Providence, had allocated, had given to the US in order to relieve the pressure of immigration. This land, our manifest destiny, our future made real, is to overspread the continent. And so that was this idea. Now this is painting that you see here. John gasped American progress, although it was painted several years later, it reflected that idea that Americans wanted to move west. And you see here the spirit of the United States moving, floating above the land. She's carrying with her in her arm a ledger showing people's ownership of land, a ledger book, and a cable for the telegraph. She's stringing along with her. And you can see behind her is sunlight, brightness, the sun rising in the east, and you see ships on the canals and on the rivers. You see railroads and settlers coming forward. And in their wake, before them, you see fleeing Buffalo, Native Americans, and darkness. So this idea that migration westward brings light, civilizes the land, and it brings new technology is all wrapped up in the painting and the idea it was trying to capture. And so if we look at this land and we look at the map of where we're moving to, settlement by about the time of the 1840s and 50s, you see that it had reached the Missouri, Arkansas, Louisiana territory. American settlement, as we know, was already in Texas. And Texas, you see the broad outline of Texas. It goes all the way up to Wyoming, along the Rio Grande and all the way back down. And then what is west of that? Although you have the Oregon territory up north, it's disputed. And then you have Mexico below it. So we're going to look at this area and how we got that. Annexation of Texas had long been an issue, and it had been an issue since the Texas Revolution of the 1836 era. But people in the United States were hesitant to annex Texas for a number of reasons. But it had been on everybody's mind. So you had William Henry Harrison in 1839 who tried to get Texas annexed, his vice president who took over after he passed away, also wanted annexation. And there were other people as well, John C. Calhoun, a senator from South Carolina who had proposed annexing parts of Mexico because he wanted to protect slavery, was all in favor of annexation. And in fact, there was a group called the Knights of the Golden Circle who were a group of Southerners who wanted more land in what's Mexico and into Latin America, even Cuba and Puerto Rico because those were already slave-holding nations. They wanted to expand and protect slavery. And so they were in favor of annexing more and more territory. But because of this strange combination of people who wanted annexation, enough people opposed these various schemes and plans that the annexation treaty failed. And so by the time you get to the election of 1844 in the United States, annexation was a major issue. And the candidates were James K. Polk of Tennessee. He was a expansionist. His whole campaign was on expansionism. And so when he wins the election, Tyler, who's in office for a few more months until March, goes to Congress and says, look, the will of the people is for annexation. Let's get Texas annexed. And so by using a joint resolution of Congress, Texas comes into the union and all of the requirements, elections, the appointment of new judges, a governor, are all in place by December 29th of 1845 and Texas officially is annexed and becomes a state of the union then. Now, Polk saw himself as Jackson's heir, the heir to Andrew Jackson. In fact, people called him Young Hickory. He was an expansionist. And one of the things that he wanted to do was answer some of these questions. Texas was annexed in December of 1845. But the question remained over Oregon's status. Now, you see in green here where Oregon is traditionally the Oregon territory traditionally lay. But there was also claims up to this area in pink, but not only from the United States, but also from Great Britain and Russia had claims on the Oregon territory. What Polk did is he went to Great Britain and he said, we want this area. He would have been happy with a continuation of the line of 1818 straight across in order to separate Canada from the United States. Just expand that line. But because Great Britain was unwilling to negotiate over the Oregon territory, he sent in people to say, we're willing to go to war. And this battle cry that many Americans had was 5440 or fight. And you can see 5440 was the line across the top part of that red section of the Oregon territory. Now, in the end, Great Britain agreed to extend the line of 1818, which is all Polk ever wanted. And so he was satisfied that he'd gotten this territory, the Oregon territory and annexed it into the United States and signed this treaty with Great Britain. And now he had the Oregon question settled and the Texas question settled. In 1846, Polk ordered Zachary Taylor, General Zachary Taylor, to move to the Rio Grande area. Now, you see that shaded area that blue and orange shaded area that was disputed territory. Well, Texas claimed all of that land. Mexico had never recognized officially Texas's independence. And even if it did, Mexico was not willing to to allow that that shaded territory was really part of Texas. They said Texas might be independent, but only through the Nueces River. So when Taylor moves into that disputed territory down to the Rio Grande, Mexican troops move north. And across the Rio Grande, across from Brownsville, that is where they assembled on May the 9th, shots were fired in that disputed territory along the Rio Grande. And by May 13th, Polk went to Congress and asked for a declaration of war with Mexico. Mexico was now at war with the United States. Congress approved the measures of war. And by June, just a few weeks later, John C. Fremont and Kit Carson, one of the mountain men, moved into California along that northern route. The U.S. also moved ships into California's harbors and ports from Los Angeles all the way up to San Francisco. And Stephen Kearney, another U.S. Army officer moved into Santa Fe, New Mexico and marched towards L.A. So there was a coordinated attack the United States took to invade this territory. Later on, Taylor sends troops to Matamoros and on to Monterey, Mexico. Polk returned Santa Ana, who has been in exile in Cuba, puts him on a boat and allows him to sail to Veracruz into Mexico City. Now, what Santa Ana had promised was that he would end the war, but instead Santa Ana continued fighting. And finally, Taylor and Santa Ana faced each other at the Battle of Buena Vista. This led to the siege of Mexico City. General Winfield Scott of the U.S. Army sailed into Veracruz with his men and they marched up to Mexico City. And there for Santa Ana and the Mexican Congress began to flee by the end of the year in 1846. By 1848, a treaty of peace between the two nations was ready. And what the Treaty of Guadalupe Hidalgo, that's where it was signed, said was that the Rio Grande would now be the border between the United States and Mexico and that the United States would pay $15 million and take the New Mexico and California territory. But this raised questions about slavery. Earlier in Congress, whenever it was approving funds for the war in 1846, brought up a thing called the Wilmont Proviso. And this was an addendum to a bill to allocate funds. And what the Wilmont Proviso had said was that slavery would never be allowed in any of the territory that we might get as a result of the war. Now, the Wilmont Proviso never went anywhere. It was never passed. But it reflects the significance that people saw about the acquisition of more land and the question of slavery and how those two issues were tied together. As it was following the war, migration increases. And you can see here, 300,000 people moved west in between 1841 and 1867. So a lot of people were willing to move. And a lot of that was due to gold, no doubt. But the acquisition of all this new land raised questions. What is going to happen to the slavery issue? Would these new territories, these areas become slave friendly? Or will they become free states and free territories? So who's going to have more power in Congress? That's what the whole issue was over. Which side? And so the question of size was also very important. California and Texas were large areas. How are they going to affect the balance? In the election of 1848, Zachary Taylor runs, he's now called old rough and ready. He was successful in his campaign in the US Mexican War. And he was an interesting president because although he was a slave owner, he didn't allow California and New Mexico to be free territories and didn't put up any fuss over that. He was a Southerner, but he was also a nationalist. And in his policies, he did things that angered both the North and the South, for example, he ignored a fugitive slave law that people wanted Southerners wanted Northerners to enact to help them catch runaway slaves. But also he upset the Southerners because when he upset the Northerners, I mean, because when there was a call to abolish slavery in the nation's capital in Washington, DC, he also ignored this. He died in July of 1850. And that brings an end to the question of manifest destiny, because it was now complete. And the question of slavery is going to continue to linger and very soon will explode. So what was manifest destiny? Well, it's the idea that the United States should expand from coast to coast. How did the desire to expand westward drive the US into war with Mexico? The United States, either by paying for it, or by going to war was willing to gain possession of this land between the two oceans. And finally, what was the effect? Well, securing possession of the Western territory allowed the opportunity for continued growth and development, of course, but it also created a severe cultural and political conflicts that are ultimately going to result in civil war. So I hope that's what you got out of this lecture. And as we move forward, we'll explore these themes a bit more. Thank you very much for listening.


Is social media manager actually a good career? That's what I'm gonna be talking about in today's video. And if you appreciate me doing this topic, go ahead and gently tap that like button and let's jump into it right now. So this video is gonna be an overview of what you'd actually be doing as a social media manager, how much money you'd make, the job description, the job satisfaction, the demand, and other X factors that you probably wanna know if you're considering going into this career. And then at the very end, I'm gonna give it an overall score from one to 10 based on those criteria. So what exactly is a social media manager? Well, according to Google, they're responsible for overseeing a company's interactions with the public by implementing social media platforms content strategies. And basically what they do in layman's terms is they post content on social media platforms like Facebook, Instagram, YouTube, Twitter, Snapchat, TikTok, et cetera. And this content is designed to build the brand and connect with followers. And from there, of course, you can sell things to them so you end up making the business a lot more money. And according to statistics across the internet, as well as my opinion, content marketing is currently the highest ROI type of marketing. That means for every $1 you put into content marketing, you're getting much, much more in return. And it's estimated to have something like 30 times higher ROI than paid ads. So there's a reason why companies are investing more and more money into their content marketing strategies. And that's why more and more companies need social media managers. So let's talk about the earning potential for social media managers. They make about $57,000 a year according to Glassdoor. But you can make much more than this depending on what you specialize in. And there's also a lot of opportunities for advancement. For instance, senior social media managers make around $91,000. And besides planning and posting the content itself, you can also work on skills such as influencer marketing management, community management, content writing, design, and data analysis. And of course, I'm biased but one of my favorite types of social media managers of course is going to be a YouTube manager. I know that most people don't think of YouTube as a business but there are many YouTube channels out there that are making tens of millions of dollars a month. And guess what? They probably need someone to manage the channel. And so you can do the math there. If you can take a bunch of time off of the YouTube channel owners back you can end up making a ton of money because they will be glad to pay you $100, $200,000 a year if you do a great job. And I'm actually going to be doing a one day workshop where I basically go over everything I know about becoming a social media manager. And I'm going to specialize specifically in becoming a YouTube manager because that's what I know the most about. And this is going to be exactly three days after this video post. So 72 hours after this video post information is going to be down in the description as well as the pinned comment below on the exact date and time. And this is going to be a live Zoom session with me where I present all of the best information, share all of my secrets on it and then I'll also answer any questions that you have. And I wanted to make this affordable to basically anyone out there so it's at a ridiculously low price. Again, check the link in the description down below. But yeah, I thought it would be awesome to do a live Zoom session and get to meet a bunch of people who are interested in becoming social media managers. And I'll tell you how to get into social media management but I'm also going to specialize even further on how to become a YouTube manager. So like I said, definitely check that out. I'll put it in the link down in the description as well as the pinned comment below and I'll be excited to meet you if you decide to do it. And by the way, if you have to miss it for whatever reason, like you're working during that time or anything like that or if you view this video past the time that I actually recorded it, that same link will take you to the recordings. So overall, when it comes to earning potential this is an amazing skill to learn in general. There's also tons of other careers that you can pivot into that are also really high paying.  It's very good. Next, let's talk about the satisfaction. So according to career explorer, this one scores in the bottom 42% of careers. So it's basically in the middle and it scores about 3.1 out of five stars. And according to pay scale, this one has a 36% meaning score and a 63% high satisfaction score. You can compare that to a really bad one as well as a really good one. And you'll see that it kind of scores in the middle as well. It scores lower on meaning, but a little bit higher when it comes to job satisfaction. But with that being said, what's hidden in the job satisfaction scores is the fact that you're learning in demand valuable skills and it's very easy to switch into a different job. Whether that means progressing up into higher levels that are related to social media management, specializing in different types of social media management like becoming a YouTube manager or completely switching careers and going into something that is not necessarily related. The skills you learn here are going to be very valuable. So that's gonna give you a ton of freedom to pivot to other types of careers, which in the modern economy, let's be honest, most people are not gonna be working in the same career for 40 years. So having that freedom to be able to pivot to other careers is incredibly important. And that's something that's hidden in these stats. So overall, I'm gonna give this one a relatively good satisfaction score, definitely not as good as some of the careers that I talk about, but still it's not bad either. Next, let's talk about demand. So social media managers are projected to grow at about 8% over the next 10 years. The average for this is usually around 4 to 5%, so 8% is excellent. And according to Glassdoor, there's about 15,000 positions open right now for social media management. And according to BLS, marketing related careers are growing at about 10% over the next 10 years. So there's a ton of demand specifically for this job, but there's also a lot of demand for the skills you learn by doing this job. Like I said before, content is probably the highest ROI investment that a company can make when it comes to marketing. So obviously companies are smart. If they know they're getting a lot of money back for this investment, they're gonna hire more people to do it for them. So yeah, when it comes to demand, I'll give this one an excellent rating. And now let's go ahead and talk about X factors. And this is basically anything that I didn't cover in the previous three that I talked about. So for instance, how easy is it going to be to have AI automate this or how easy is it going to be to outsource this? Well, when it comes to AI automation, because of the fact that it does take a lot of creativity and you have to kind of analyze things and see what's doing the best, this is not one of those tasks that you can easily automate. And when it comes to outsourcing, the great thing about marketing related jobs is typically you're gonna have a huge advantage if you hire people who are in the market that you're selling to. So obviously the biggest cash cow market in the world is the United States market because we have so much money here. And so companies are always going to prefer to hire people from the United States. Another thing to mention here is if you get skills working with a brand, working on their social media, it's gonna be a lot easier for you to start your own social media at some point as well. So if you're the type of person who wants to start a business in the future, my number one recommendation is to start making content. Making content makes every other aspect of your business so much easier. This is something that Gary Vee's been saying for like 10 years and he was 100% correct. This is why you have guys like Alex Formosi who was making like $50 million a year and he was a kind of a paid ads expert completely shift his business model to spending almost all of his time and effort making content. This is why you have podcasts like the all-in podcast which is four different billionaires. I think one of them is like only a hundred millionaire but you get what I'm saying. Four different billionaires spending their time and effort making a podcast. There is a reason for that. These people are super selective with what they spend their time doing and they are choosing to make content. So if you're the type of person who wants to learn in-demand skills and then switch into something else in the future that's either maybe like in a higher level position or start your own business, this is gonna be great for you. So you have to add that into the X factor score as well. And overall, this one has a great X factor score. This is one of the most valuable skills that you can learn. And then the overall score is going to be 8.5 out of 10. This one used to be better about five years ago but I think what's happening is people are wanting the social media managers to kind of specialize in particular platforms. So if you specialize in a particular platform I think you're gonna be even more valuable. And that's why I'm doing this one day workshop where I teach people how to get into social media management but I specialize in teaching people how to get into YouTube social media management because that is what I am an expert in. And like I said, it's gonna be a live Zoom class with me. I'm going to present you all of the good stuff, all the secret sauce and then I'm gonna answer any questions that you have as well. So I'll put a link down in the description as well as the pinned comment below and I hope to see you there. By the way, if you haven't checked it already I did make a remote job tier list and you can check that out by clicking right here.


OK, so I was told that maybe a lapel mic would work better. Can you hear me OK? Good, that means I can wander. What was that? OK, I'll do it this way. Since I only have 10 minutes, I'm going to jump right in. There are different levels of analysis we're doing in a clinical laboratory from targeted exons that we may seek for known specific mutations. Many times there's a single gene that causes a disease that we could look at it. But we have now entered the period where we do gene panels. And some of these can get quite large. And so we're dealing with multiple genes, multiple variants to be able to work through. And we are very close to the time of a clinical whole exome type test. Traditionally, our regions that we interrogate are the exons, intron-exon boundaries, known intronic mutations, and if known gene regulatory regions. We do not do the deep intronic mutations unless something more is known. So we don't want it to come up with tons of variants of uncertain significance that we know we can't handle in the first place. So I wanted to bring out American College of Medical Genetics recommendations for laboratories that says that the laboratory must provide the interpretive information and a best estimate of clinical significance for the variants. So it is a responsibility of the laboratory. And so it starts in the clinical lab when we write the report for the patient that we try and give the best estimate and the best classification that we can. On the side here, this algorithm is actually with that paper. And you can see it's fairly complex. So I'm going to try and simplify it a little bit. Basically, we look to see if it's been previously reported. And if so, does it tell us whether it's pathogenic or benign or somewhere in between? And what is enough evidence to collect to be able to classify it as pathogenic? We need to realize, and I can't emphasize enough, that in a clinical lab, we're dealing with a lot of different diseases, a lot of different genes. Each of them have their own little quirks about it, I'd say. And we need to realize that if it's autosomal recessive versus X-linked, we're going to approach it differently. We're going to approach dominant versus recessive diseases differently. A simple, basically, if you have a dominant disease, you look for one mutation that is causative. If you have a recessive, you have to find the two mutations and show that they're on different chromosomes. If it has been previously unreported and we can't find it anywhere, then there are many times that we can say, this is expected to be pathogenic. It's a stop codon and nonsense mutation in the middle of the gene. And we will report it as pathogenic. If there's less certainty, we'll say it's suspected pathogenic, uncertain or suspected benign. And then we are getting to the area that we're doing some further classification on severity. Not often, but at times that we can talk about severe, moderate, mild, or even very mild mutations. An example of this would be pancreatitis, that we're looking in the cystic fibrosis gene CFTR gene to find mutations that do not cause cystic fibrosis but cause pancreatitis. And so we need to put together a severe mutation with a mild or a very mild mutation to be able to give a report with that. So there are times that we need to classify more than just pathogenic. We need to talk about severity. So this is our process right now. We check our internal databases. Have we seen it before? What have we classified it before? What evidence did we find before? So we don't have to repeat that work. Now, the problem here is that there's going to be differences between different laboratories because I'm going to see different variation than another laboratory. And so you may get differences between the different laboratories and we need to become more standard. I do look at local specific databases. And I'm including the biobasin with this. But again, there's differences between databases. Some of them are great. Some of them I really like. And other ones are only marginally useful. Some of the differences are that the evidence is given, that they may not give much evidence. We don't know how often or sometimes they're not updated as regularly as what we would need. And as Les pointed out, we do need to check original sources. Simply finding a mutation reported in a paper, one time I pulled it up. And that mutation was actually found in the control population, not in the disease population. But it was labeled as pathogenic in the database. We look at DBSNP and mainly looking at the frequency. However, we need to be cautious about it because they're a pathogenic and benign, both in DBSNP. One of the thoughts, though, is that if we're looking for a very rare disease and we find it with a high frequency in a population, it's probably not the cause. On the other hand, if we're looking for more common diseases, especially if it's recessive and we find it in the DBSNP in a fairly high population, that doesn't eliminate the possibility that it's pathogenic. We do look at prediction algorithms. However, this is more of a warm and fuzzy, and I haven't yet classified anything based on simply a prediction. I do do a literature search, and I'm not above Googling. However, my problem is that I don't know when to stop. If I can't find anything on it, is it because I've missed something or is there that there's nothing out there? Truthfully, I've taken up to maybe six, eight hours on a case, trying to gather this information, trying to put it together because I didn't know when I said, okay, I can't find anything, therefore, nothing's out there. This is a manual process, but we are now getting it into the fact, an automated process, which we are able to do checking our internal database, the local specific, DBSNP, those types of things. We are putting together in a pipeline so that we can come out with at least a smaller list of variants. So, as he had mentioned, there are different types of evidence that we look for, phenotype, genotype, cases with symptoms and normal controls. We look at functional studies, and that can be a wide variety of things that we take into. We will look at the splice predictors or amino acid severity conservation. What is important for us is we need to know if they've occurred with other causative mutations. And we need to put it together for recessive diseases as a combination. And we do, I do like genetic evidence and family concordant studies. These are not easy for clinical laboratories to get, however. And we have an IRB in place to be able to do that, but we've only done maybe a handful because the families just are not large enough to give us the information we would need. Now, the clinical lab can collect some evidences. It's not easy for us, but for example, we can attest some additional family members to show that a mutation is de novo. This is oftentimes in the pediatric population. The parents are very willing to work with us and get us the samples in. So we can show that a mutation is de novo. The linkage analysis larger families are more difficult. So we do go to prediction programs. And we've had a recent PhD, Bioinformaticus has been working on a project where he has worked on his own predictor using amino acid properties. But what has been useful for us is that he's combined it with other prediction programs. And in a sense, come up with a reference range which clinical laboratories like because we're used to working in reference ranges. But if you can see here by adding the scores up and he actually weights them differently that here are all of the known benign, the scores of known benigns, the scores of known pathogenic. This one uncertain falls right into there so it's predicted to be benign. Same way with this hair, this falls within the known pathogenic scores. One of the take home messages with this is that these predictions are much better and more accurate if they are gene centric and we don't try to have one set of parameters that'll cover every gene out there. A quick example, Alport syndrome because of a collagen, a defect in a collagen gene. You have an amino acid motif glycine XY. If that glycine has changed, it's going to disrupt the function. So for Alport syndrome, a glycine change is going to be much more significant than potentially a glycine in another gene. So when we try to collect additional evidence, we accept a very broad definition of functional. So for example, we will look at immunohistochemistry. Here's an example with the PMS2 gene for Lynch syndrome to see if it's expressed. So we can combine expression with a mutation that we're looking at. For biochemical diseases, we can look at either the enzyme or sometimes the pathway. For example, with MCAD deficiency, we look at acyl carnitines or we can look at transfer activity, sometimes structural analysis or going to an RNA. So these are the types of things that the clinical lab can collect. And I thought I would do just one brief case to show you what we're dealing with. So for example, this is in the PMS2 gene. We've found two different, I mean two different base changes at the same position. Now Ohio State has seven families and we've worked with them. ARUP, we have four families with this now. In Ohio State, there's been one biolilics. So if you have one mutation, then you're at risk for colon cancer with Lynch syndrome. If you have one mutation on each chromosome, so you have two mutations, it's actually a much more severe with brain tumors, et cetera, in a pediatric population. So we had a little bit more information that the PMS2 was absent by immunohistochemistry staining and it was a very unstable. So microsatellite instability was high. This is the best family that we could work with given this family. We had a 10 to one Bayesian factor or a likelihood factor that this is pathogenic. Obviously this would not fit to be classified as pathogenic if we used that alone. The amino acid predictions, polyphen, probably damaging P-mute benign, but with not very much reliability. Other ones leaning towards disease causing. It wasn't seen in 182 controlled chromosomes, which is not very useful when you're looking at very rare mutations, but it still can help us somewhat. The Western blot showed 50% protein compared to the control and haploid converted clones showed expression from only one allele. That starts giving us evidence, a stronger evidence that this is actually pathogenic. So my question, given that evidence, is that mutation pathogenic? Would you sign a report saying it is pathogenic? You're not helping me. So there is some things that the laboratory can do. We do have clients and we do get patients from many places and if we can collect some symptoms, some family history and a collection of previous lab results, we can start putting this together with the molecular results, the sequence variants, the known common polymorphisms and we are doing deletion duplication analysis with most of our tests so we can combine that information. And we do need to keep track of when we actually do have enough evidence to reclassify. Truthfully, we've done very little reclassification. The evidence to come in has been very slow. So what do we want? I want a clinically valid whole genome database. We need the variants, if the classification is known and this is probably going to have to be expertly curated, put it down. Again, if the severities are known, I'd like to start collecting that information. We need to know ethnicities and frequencies in different ethnic populations. We need to know the number of cases but it's much more difficult in a database if I see the same variant 10 times. As Les pointed out, they may be from the same family or they may be from different studies but I need to go in and look at each one of them differently. I'd like to have some type of a summary of the information. Obviously, we need to know the symptoms. We need to know if it's in conjunction with other evidence of other mutations. The evidence is one of the things I wanted to point out so that they're not all weighted equitably. The predictions, I weight very lightly. A case control study or a genetic linkage pedigree, I weight much more heavily. So we need to make sure that we're not putting everything, lumping everything together and the risks of an incorrect classification are different between different genes as well. There are sometimes I have to be much more cautious in calling something pathogenic. So the criteria for calling them pathogenic may be slightly different between different diseases. And because of that, I put this last is that as we're trying to do this, don't oversimplify. This is very complex. We have to take what evidence as we get. Those are not gonna be the same types of evidence for the different types of diseases. And the clinical labs are really very interested in helping and contributing but we have very few resources to do this and so any type of submission to a database based from the clinical side has to be very, very reasonable for us to do. And thank you. The university. I was wondering, we've talked about this before about how each laboratory is really working on this within their own kind of area of silo, creating their own databases and own resources of how to analyze these variants. Can you talk right now about the kind of collaborative efforts between the laboratories if they exist and also just on a day-to-day basis? Do you pick up the phone and call another director from another laboratory to try to work through some of this? The answer to the last question is yes, I do on occasion. Not often because we're getting enough data in-house but yes, I have picked up the phone and called another lab director. There actually is a collaboration going on. Donna will be speaking more about the ClinVar efforts and Heidi, Rem in the back, has really organized a large number of laboratories who have committed to be able to work together and submit the information they have to a central database. So there are definitely efforts moving towards that. You mentioned that you're working in an IRB to be able to interact with patients. It seems to me that, you know, what's made of it is welcoming talk where we're really crossing the line and really doing research on collection. And it seems like one of the things that would be important to discuss as part of this meeting is can there be creation of a safe harbor that would allow the exchange of information from the clinical side to the laboratory side in the context of trying to generate knowledge around these variants? Is this something, I'm sure it's something you've thought about but to have you in your discussions with others come up with mechanisms by which this might happen? We've had at length discussions with our IRB and they have basically told us that if we want to simply look at an individual for their mutation and not make it more generalizable, we can do that within the clinical realm. As soon as we want to say this mutation is now pathogenic and if we see it in family B, we want to call it pathogenic based on the evidence in family A that becomes human subject research. I don't agree with them to that extent and we've had discussions of what is research, what is clinical and at what point are they starting to step in into the clinical practice. But that right now is the definition and so if there is something that we can do to take back to the IRB and say these are some consensus that I can take to maybe loosen up some of these requirements, it would be very nice. So this is something that could potentially lend itself to say a conversation with the Office of Human Subjects Research which will guide us to say this is actually something that we would consider to be exempt. I'm assuming they would agree with that of course, but in terms of things to take away from this meeting that the NHGRI would potentially lead, it seems like this might be a reasonable conversation. Yeah, one of the problems is that for some reason, whenever our IRB sees genetics or genomics, all of a sudden it jumps it up to a high risk. Elaine, you mentioned sometimes thinking differently about a variant depending on the clinical context that you were having to evaluate that. For instance, when we have a prenatal case and we know a termination might be based upon our classification and so we're very cautious about that but it gets us into a dangerous situation where we're, I think, differently evaluating a variant's evidence depending on an outcome when I think our goal is to really be more standardized and there's so much literature out there that's incorrectly classified variants because of using non-standardized and not high threshold. So I wonder if you could comment a little bit more on that challenge. Yeah, so for example, we do a lot of beta-globin sequencing. A lot is known about the beta-globin sequence and there are other tests that we can use to tell them that they have something going on. So my molecular genetics test is not the one and only criteria they're going to use to take any action. It's only one piece of the puzzle. On the other hand, something like hereditary hemorrhagic telangiectasia that we have kind of a center of excellence there. A child may not have any symptoms at all but this could be potentially, this is life-threatening and so if we tell them that it's benign or likely benign, they're going to stop doing the intensive monitoring of that patient because this is the only bit of information that they're going on. So I guess in my mind it's a question of is this being used as the only source for an action or a lack of action or is it combined with other laboratory types tests that can also help guide? And so maybe that's in my mind. So for example, our genetic counselor with HHT said I want the greater than 99% probability for you to call it pathogenic. Well if I put that criteria to all of our reports in every disease we have, I probably wouldn't be reporting out many pathogenic mutations truthfully.


Hello and welcome to a simple Lora1 demo running on STM32 WL. What do we need for this demo is a modified repository example called the end node. The original example needs credentials and commissioning for registration of the specified node and communicates with the things in the network server and can make devices to show the data. We will simplify this example so that it will simulate an AC unit and we will send over the network only measured temperature and we will receive either increase, decrease or equal temperature so that the application will tell us how to control the temperature. Then we need STM32 WL nuclear which we will flash with this simple application and right after the flashing it will be able to send the data to the Lora1 network server. In order to send the data to the network server we also need a packet forwarder. For the packet forwarder we will use the STM32 F7 nuclear plus rising HF module by simply connecting it to a PC. Then for the network server we will use an open source solution available in GitHub which is running directly on this local PC and can run on any other PC. Lastly for the application server we will use a node thread flow and it will simulate our AC unit. It will accept all the nodes that want to connect. No registration is then needed and it will run the application meaning it will provide us the increase, decrease and equal control. Now to the data flow. First of all the STM32 WL SDN node will send out the measured temperature which will be then forwarded by the simple F7 gateway and then over the Ethernet it will go to the laptop running the network server. And finally through MQTT it will reach the application server which will decide which control scheme to select, increase, decrease or equal temperature and it will get sent again through the whole system back to the STM32 WL. To start with the demo we will first of all load the application which I have prepared in a binary format and we will simply flash it by using the mass storage of the STL link on board of the nuclear. So we will simply copy it to the end node. It will reset and start the application. Then when I connect with the console application I will see what the hardware is doing. It is attempting to connect to my server. Now it has joined. Now it will send some data over to the network server which I have running right here. Here is the latest join I have just attempted. As you can see I have some trials from before where I have already a response for the data. In a couple of minutes there should be a response there should be a transmit data from the node to the network server. So it will get here and then it will be passed to the node for the application. As you can see here is the data received. It is parsed and now with a simple threshold which is maybe too low for this particular application. It will get compared and it will send some message. It is again parsed into JSON format and then it is sent back to the network server. Then the network server will handle the sending to the end node. Here is also the packet forwarder which is initialized already. It is actually transmitting anything that goes through. As you can see now the data has been sent. We have sent some data and then we have received that the application should decrease the temperature. As you can see it is on the dashboard as well. Send some data and then it receives some response from the backend from the node red. Here it is also visible that the device with this address was sending some data. The materials to this demo will be provided on the Google Drive available on this link. It will be available after the 12th of November. It will contain not only the binary but also a tutorial on how to set up your own network server and application server. So this is how simple it is to send some data over Loreman network with STM32WL. Thank you for your attention.


My name is Allison Skinner, artistic director of the Davis Corral, a non-profit chorus serving Davis and the surrounding communities. We believe music elevates the spirit, so we perform choral concerts for the community throughout the year. I perform a wide variety of great classical literature, from familiar standards to more contemporary compositions, to create a performance experience that everyone can enjoy. We have a lot of fun during rehearsals getting to know the music and each other, and we strive to show our enthusiasm for the great music we perform in our concerts. Learn more about us and our upcoming performances at daviscoral.org, or call<PHONE_NUMBER>.


What happens in the debating chamber? All MSPs can take part in meetings in the debating chamber. As well as discussions on proposals for new laws, chamber business can include debates. These are discussions between MSPs, normally based on a motion. First Minister's Questions This is when MSPs question the First Minister. Question Times These are sessions when MSPs question Cabinet Secretaries and Ministers. Ministerial Statements These are used by Cabinet Secretaries and Ministers to inform the Parliament about urgent matters or to make announcements on policy. Decision Time This is when MSPs decide on the motions discussed that day, often by voting. Are seats in the chamber allocated to specific MSPs? MSPs are free to sit wherever they like in the chamber, however they usually sit in their party groups. The presiding officer, or a deputy presiding officer, sits at a desk at the front of the chamber, facing the other MSPs. What is the role of the presiding officer in the chamber? The presiding officer, supported by two deputy presiding officers, chairs meetings in the debating chamber. When chairing meetings, the presiding officers must act impartially.


argument seriously lied, I think it is. Yeah, a lie down with a kid. A calf and the shell graze, a young shall lie down together. Some people have dreamed this kind of utopian vision of a peaceable kingdom, but on the surface at any rate, naively, this is completely ecologically illiterate because Ina gene has lifted upwards and tragically so many people today do no false allays and we know. I'm advancing. Yeah, this is it. Anyone who is. If I'm advancing too quickly, just let me know. It is fantastic of reality and whether they believe in the election of a good life. Excellent. At this stage, would you like to take some questions from the audience? Actually, you're you're holding up quite well. So what, because of the fact that you can't directly hear what people are saying, I will basically repeat the question that I hear coming from the audience. Okay.  All right. I got one. Yep. Richard Harvey joins with a question of his just a minute. I'll say really quick too. So we've got a bunch in the chat and then we'll let people kind of go. Maybe if you can also like raise your hand, there's a little option to do that. And oh gosh, how do I get back there? So Michael, and I'm going to rely on you to if any additional questions need to be shared that are in the chat or whatever, I can't click anywhere. I've got my hands full here. So if there will take questions from the audience, but if you want to interject, just go ahead. Okay. Okay. So we'll we'll let Richard go. And then I've got a few from Jenny D and from Trevor and from Connie. And we've got about 1520 minutes to do this. So go ahead, Richard. Okay. So we have about 15 or 20 minutes here left with you, David. And I'm now hearing Richard's question. And I will relay that to you. Okay. So for everyone who can hear me directly, there's a great sequence in Hitchhiker's Guide to the Galaxy where a cow that is very much looking forward to be butchered because it's been caused to experience the process as intensely pleasurable and also has been made as intelligent as a human comes out and tries to get itself eaten. And it's a really funny sequence and everyone should read Hitchhiker's Guide to the Galaxy again. So question, why not create a biosphere where natural processes as they exist now are not merely subverted, but are simply made pleasant or preferable to organisms that are presently victims of suffering because of the natural order. So Richard asks, why not create a biosphere in which the things, and I'm already forgetting some of it, but the things that are presently happening to the organisms in the biosphere become pleasurable to these beings instead of what was the alternative, Richard? Entirely subverting processes as they exist. Instead of entirely subverting processes as they currently exist. Yeah, thank you very much. So I'm going to share with you that ironically or serendipitously Richard shared the cow, the anecdote of the cow that wants to be eaten in Hitchhiker's Guide before he asked the question. So you guys were both thinking on the exact same wavelength here. I have another question. So this comes from Janati Stolarov and he says, given that it's meat consumption is still highly advisable for health, he says, basically, do you think that the widespread advent of lab grown cruelty free meat will be the necessary step to convince many people who are not comfortable with veganism or vegetarianism to basically go for that? Do you think that this will be the key component for a society-wide shift toward avoiding the killing of animals for purposes of food consumption? Thank you. So because we only have time for one or two more questions, what we're going to ask everyone to do is to post additional questions that they have in the campfire and I can't guarantee because of the technical difficulties that we are experiencing that David will be able to stay on the entire conference, but we will make sure that we relay these questions to him and get his delayed responses. David, we'd love to have you stay on if you can figure out what's going on with your audio, but we totally understand if the conference experience isn't sufficiently blissful for you. Excellent. So what we'll do then is as we receive questions for you, would it be all right if we shared your email as well with the audience? Okay, excellent. Okay, so we will share both the PDF and your email, but in the meantime we've got time for one more question and I'm going to go ahead and ask this one from Jeremy Hadfield. He says, what is suffering or to state the question more technically, how do you know if the mental state of a sentient being other than yourself is suffering? Understanding what suffering is seems to be a prerequisite for negative utilitarianism's project of reducing suffering, but I don't think there is a strong technical definition. How do you determine if you have reduced the suffering of a non-human animal with any epistemic confidence? Magnetic radiation, lumps you with animals, essentially to Prince of Russia uniform.