Welcome to Toffy TV, it is my three things from Eddarton 2, Brighton 3 another hugely disappointing afternoon at Gooderson Park, first game of 2022 and Eddarton will lose it like they have done in the previous four seasons losing the first game of the new year and today was appalling really to be honest it was just shambolic from the moment the team was announced when the final whistle went, absolutely abysmal afternoon at Gooderson Park, the first thing I want to talk about is the formation, the manager, seeming to enjoy making things more difficult than they need to be, obviously he is falling out with Leucardine, seems there is no way back for the French international he was on the bench today but I think if those two were declared the differences he'd have started and if he wouldn't have started he'd have certainly came on a half time and he didn't so I think we can read from that, that looks done so I think from an Everton perspective it needs sorting out very quickly in terms of we need the bidding from whoever wants to take him and then off we go, we reinvest that money because quite clearly under Rafa Benitez he hasn't got a future of Everton, Leucardine probably doesn't want to stay and if he's going to go to Newcastle which is one of the teams who are after him then it shows the lad's ambition and it's probably more about money but the formation the manager picked today was appalling five at the back a home to Brighton who had won one game in 12 absolutely embarrassing to go with a formation like that to make it worse he had Seamus Coleman and John Joe Kenny in the same team with Seamus Coleman playing at left back which was again embarrassing and Seamus is struggling to play right back nowadays to ask him to play left back is just pathetic the last game I can remember him actually starting at left back was his debut in Ben Fieker and he lost 5-0 and he was torn apart that night but he was a young lad and he was torn apart by the likes of Angel Di Maria and Javier Euler and people like that, not by Brighton's players but he struggled today and Kenny had a go and had an assist I think he did have an assist for Gordon for the second goal but he wasn't great either but to play Seamus at left back was embarrassing like I said on my mat reaction you can start with an idea thinking we will try and get round it doing this that but if it doesn't work or it's clearly not working have the balls to change it and he didn't we were 2-0 down after 25 minutes against a team that hadn't really done anything it was just it was ridiculous and he just left it sat on his hands and we were playing flat back 5 a home to Brighton who had one striker it was incredible to watch really but not for any good reasons and I think all he's doing is making things more difficult than the art we're already very difficult we've had we have been decimated with injuries this season even today you know we had no Yeri Mina the Llywodraeth thing is doing all him and Llywodraeth is doing so whatever but we haven't got our first choice left back we haven't got our best centre back we haven't got Andrews Tarn's end who's injured we haven't got Richard Charleston who's injured but a lot of the other players play a lot of the other players are our first choice players ok we had Dominic Alvallun starting his first game in 4 months so he was off the pace but I thought he'd done alright one of few headers held it up had played his part in the second goal blazed the penalty over the bar in the first half instead of just hitting the target he'd be done alright so we're getting players back to fitness but we're still like you say we're still short on those three Jarapranthwaite it was injured as well which you know the lad scores a chelsian does well and has picked up an injury in the two and a half weeks that since we played that last game and that's the way the season's gone it's gone wherever anyone's tried to get some momentum they got injured and it's been like that from day one the manager is making it much more complicated than it needs to be Ben Godfrey's played that left back and did it quite well last season and he's done it a few times this season he's been alright he's not amazing there but he's ok and yet today he decided in his wisdom to change it all again why didn't he just play? he wanted to play Coleman if he thought 3 for 5 at the back was the right way to go about it why didn't he just play Coleman as the right centre back? I've whole gate a whole different story but why didn't he just do that? why did he change it so much? why was Dominic Calvert-Lewan totally and utterly isolated? you know Damari Grey's having to pick the ball up miles away from Calvert-Lewan and then go on a chink and run Anthony Gordon for the shame it's not good to watch Brighton knock it round though they're not amazing and they do it with I would say you know with average players where I presume as a good player don't get me wrong and you know Maupai works hard they're neat and tidy they're not amazing but Grey and Potter's got a way of playing they all buy into it they all work hard they pass it round a comfortable in possession we don't really have anything if your game plan is to smash it up to Dominic Calvert-Lewan and hope he can head it then ok that's fine if you look at it and go listen we haven't got the tools to do what they're doing then put people round them so that when he wins it or he knocks and he won a few today he flicked them on into space and if we'd have had a runner we'd have been in on goal or he'd have had someone to pick it up and it was embarrassing and I don't know what his thinking was and we lost the game basically because he put us on the back foot so shocken by the manager today absolutely appalled by the formation and he's making it very difficult people don't like him anyway and each week less and less people are believing in it and it gets to the stage where you're looking and saying there's just no way this is going to work because if he can't get the fans onside at home then he's struggling right now and I know listen it can change in a heartbeat but right now where are ever and getting seven wins from he just can't see it and I know listen it can change very quickly because he could win three games on the run and all of a sudden the complexion the whole thing changes massively but other teams are winning games Brighton if we don't want today we were we'd have gone two points behind Brighton I think we don't want today we'd have been 11 points clear of allegation and four wins is huge at that stage but we haven't we've lost again we're in trouble and it seems we are in trouble and it needs sort and he needs to either sort it or we need to make a change and he's not giving himself a chance because he keeps making stupid stupid decisions. The second thing I want to talk about is the defence very briefly on this we've had five defenders in there today and we've conceded three goals against the team that doesn't score a lot of goals. We you know we tried this formation before okay at Chelsea I kind of understood it because we were always going to be on the back foot we were always going to be up against the team that would have all the ball who would be creating chances and we had no players so I kind of understood it. Today was one of our strongest benches we've had this season and now listen I'm not saying the quality is amazing on the bench because it wasn't but we had a lot of players today on the bench who played in the first team and yet he went with a formation that was bizarre and one way people were out of position but that defence is appalling. Michael Keane was dreadful today like properly dreadful even things like thinking he could score a left footed half volley in the 91st minute 40 yards out was just he was pathetic all over the place. Garbage Mason Holgate was putting a garbage display today shameless wasn't very good but he was left back walking the Ben Godfrey for me was all over the place. Midfield were overran you know but those defenders at the back you know brightening waft for two teams you've never won a good isn't in the histories. I've got scored eight goals past us at home this season and okay Macalester's second goal was a tremendous strike but we could have done better with it.  We've just bought vitaly Michael Enco he's coming in or Michael Enco he's coming in is he going to solve all our problems at left back asking the kids to come from the Ukraine and into a team and just let goals in for fun. Nathan Patterson if we can get that over the line this week does he does he solve all our problems you know in the defence hopefully hopefully but you're putting a lot of pressure on a kid. So there's real problems there and the manager needs to sort it out playing five at the back is not the answer playing three in the field we can give you a bit of protection and also help your team of the other might be more of an answer but the defence has got to sort itself out very quickly.  Finally let's finish on a positive Anthony Gordon who was the only real positive I would say to that obviously having Dominic Alvertley when back was a huge positive and he'll get better over the weeks of course he will. Damari Gray I thought was good in the second half but Anthony Gordon I think ran himself into the ground still does things poor choices of time but he's a kid scored two goals won the penalty in the first half put a couple of great crosses in ran his socks off for us. So here's the real positive. The goals will help him massively that'll be a big boost to his confidence but he can't do it all on his own and we need we need better round and we need to help him out. But it was he was the real plus point today and it's a pity for him that we haven't got a more positive result with him getting his first goals but it's testament to him that the gladus reeds sung his name he was the only one really that got clapped off at the end. Gray got one as well but he was the one who the crab was singing his name and rightly so he put the effort in today and we're ultimately to defeat it another disappointing defeat against a team who I thought we could beat at home. I thought we could get maximum points and kickstart ourselves hoping coming off the back of the Chelsea game but it seems to be one step forward and three steps back at the moment. Until that changes everything going to be in a real battle at the wrong end of the table and right now you do start a worry. We're almost halfway through the season. We've got 19 points from 18 games which is appalling absolutely appalling but considering we had and then we had 13 points or something after about five or six games so it's absolutely appalling. One win since September 25th. Terrifying. Absolutely terrifying. There you go. There are my three things. There are my thoughts. I've rambled on far too long so happy new year to everyone watching. Let me know what you think in the comments section below and I'll see you later.


Okay, so this webinar series consists of a total of four sessions that will take place in April and May. As you know, the Galaxy ecosystem is very broad in scope and reaches different audiences, each one with different needs. So the aim of these four sessions is to show to those different profiles which resources are available and useful for them. And today we will focus on the researchers and in the upcoming weeks we will go through training and education, tool development and admin roles. Next please. So I'm happy to introduce to our speaker today that is Dave Clemens. I guess that Dave needs no introduction. But for those that don't know him, he has a background in computer science and he is now for more than 10 years, a worldwide community coordinator for Galaxy. He has a very extensive experience in all community-related topics, training, outreach. So Dave, thanks for presenting today. I'm looking forward to learning from you as always. Thanks, Ben. Good morning, good evening, wherever you are. So thanks for spending some time with us today. Let's see. Let's talk about our goals and what our not goals are. So our goals for today are for you to learn where and how to find the resources and help you need to use Galaxy successfully. So a lot of lengths today, a lot of pointers to stuff, not spending a huge amount of time on any one thing, but a lot of resources. Okay, not goals for today are to teach how to use Galaxy. Don't worry if you're brand new to Galaxy or if you only know a little bit because we will highlight resources for that today. How to learn how to do that and how to become an expert. But the goal of today is not to teach you how to use Galaxy, but rather to give you resources you can come back to next week when you have a question. And you vaguely remember hearing something about X. So that's our goal for today. Okay. The slides are at this URL. They're now linked to from that URL anywhere. Anyway, and this is important. Again, because of our goal, we're going to link to a lot of resources today. And so a week from now, you want to be able to find these slides, find those links. So bit.ly slash gr4-researchers. We'll take you to the event page for this which has a link to the slides. So very important. Okay. So before we dive in, two or three slides on what Galaxy is. Projects definition. Galaxy is an open web-based platform for accessible, reproducible and transparent data analysis. This reflects our core goals and this is what we aim to do. But it's possibly not how most people think of Galaxy. And I think the way a lot of people think of Galaxy is a lot closer to Keith Bradenham's definition. Galaxy is a web-based platform that provides a simplified interface to many popular bioinformatics tools. So put another way, what Galaxy does is protect researchers from the command line. And it protects you from Linux systems administration. So you can use Galaxy to do sophisticated analyses. You don't have to learn what to do when LiveZ does not install on your system. You don't have to learn how to use the command line. You can do everything in Galaxy from a web browser. Okay. So again, two definitions basically different sides of the same coin. But it's worth pointing out. So this only captures half the picture though because equally important is that Galaxy is a community. And this is one of my favorite quotes. James Taylor was one of the founders and PIs of Galaxy and this is from a keynote at the 2019 community conference. The most important thing about Galaxy is this community. And as we go through slides today, you're going to see that it really is a huge community and that community contributes an awful lot. And we really support each other. And so keep in mind that we are not just a platform.  And without that, we would not be successful. So I cannot stress this enough. We are equally a platform and a community. Okay. So today is divided into three sections. One is learning how to crawl if you're brand new to Galaxy. This is the shortest of the three. So if you're new to Galaxy and even if you're not new to Galaxy, your absolute best friend is the Galaxy Training Network. And I want to spend some time exploring that. So we're going to go here, which is traininggalaxyproject.org. Okay. And I'm going to zoom in a little. Okay.  And in particular, I recommend starting here, Introduction to Galaxy Analysis. I'm going to zoom in one more time. There we go. Introduction to Galaxy Analysis. Start there. And I clicked on that. There's the core material. There's also some stuff down here about R. So you can run R in Galaxy using RStudio. And this tells you how to do that. And these get really rave reviews, by the way. But let's focus up here for now. There are lessons. And for each lesson, there are a number of things it can have. So some of them have slide decks. Some of them have hands-on tutorials, input data sets, workflows, and Galaxy instances. We'll cover most of that right now. So let's start with slides here, a short introduction to Galaxy. Let's click on that. And it brings up a slide deck. And you can walk through this and learn what Galaxy is about. Okay. Or whatever the topic is. So this is just about Galaxy in general. I'm going to use the arrow keys to move forward and backward. So basically, Introduction to Galaxy. Note at the bottom here it says Press P to view the presenter notes. So we can do that. And this will show us what the presenter notes are. So you can actually teach using Galaxy. And in fact, that's what next week's webinar is about, is Galaxy Resources for educators and trainers. And they will talk about this as well as many other things. But there are extensive notes here. And you can use this to present materials. So I just hit P to get out of that. Okay. We're going to go back to this. So there are slide decks. The next thing I want to highlight is these little down arrows. And they're available on the hands-on and on the slide. If you click on that, it says you can look at this in French or Japanese or Spanish or so on. And so I'm going to try French. I'm going to click there. And it uses Google Translate to do that. Okay. Now that's going to work mostly. Okay. But we do some extra stuff to make sure it works. When the slides and the tutorials are developed, the authors try to use simplified, straightforward English so that Google Translate can cope. Okay. And so in theory and usually in practice, what you get is useful translations from that because we actually do put some effort into making sure it works. Let's go back. So slides, multiple translations are available. This right here, I think this is the, is that the only one on this page? Yes. That's the only one on this page. Some slide decks are also available as videos. So I'm going to click on that. I think I am also sharing my sound. So hopefully you'll hear this. And it brings up a video of the slides. And I'm going to play this. A short introduction to Galaxy. What is Galaxy? So what you're hearing there? Are you hearing that, Bea? Yes. Yeah. Oh, good. Okay. It's a pleasant, vaguely British voice. And that voice is automatically generated from the presenter notes that we saw earlier. So some of the slide decks, and we're improving more of them as we go, some of the slide decks, a lot of thought has been given to those presenter notes so that you can automatically generate these videos. And the text shows up here. So you can actually hear what's being said. And all of it is driven by the presenter notes. And all of this happens automatically. So it's kind of magic to have that. Okay. So lots of videos, lots of slide decks. Let's come over here to the hands-on. Again, available in translation. I'm going to click on this. A short introduction to Galaxy by Anna Syme. Anna is in Australia. She is helping with the plant transcriptomics training that's going on this week worldwide. She's also doing a workshop, I think, today in the Australian sense or maybe tomorrow in the Australian sense, because I'm in the US and the dates are confusing. But she's doing a workshop this week on plants as well. So this is what a typical tutorial looks like. There's an overview at the top. And then we scroll down and hear this. Since this is the introduction, it tells you about what Galaxy looks like, three-panel interface, all the things you can do. And then it comes down here and there are hands-on sections. And it says, okay, name your history. And it tells you how to name it and where you type it. And it walks you through. So these tutorials are a mixture of why you're doing it and how to do it. And they have comments. They have hands-on. I'm scrolling down. They have use a tool like here, FastQC. Right there, that is a tool for looking at the quality of reads and here's some output from FastQC. Okay. So there's also questions here. I think I saw one. No, it's down here. Anyway, there's a lot here right there. It's questions, solutions. And it's really thorough. And it's a great way to get introduced to Galaxy. So I'm going to go back right there. Okay, so that's hands-on. And there are a lot of these. I think our current number of lessons is over 150. Maybe over 170 by now. They mostly have input data sets. And these are stored in Zenodo. So they're online. If you ever create training materials, you know, creating the input data sets to use is actually very challenging. And the tutorials will all have links to those. And you can also get them directly from Zenodo. The last thing I want to talk about here is this last one. So Galaxy is open source software. It's been installed in hundreds, if not thousands of places around the world. Some of those are public instances. And what the training network does is it walks through this tutorial and sees what tools it needs. And then it walks through the public instances of Galaxy, of which there are over 100, and says which of these support the tools in this tutorial. Since this is a pretty basic one, there are a lot of public servers that support it. And you can pick any of these. So if I click on High City Explorer, it'll take me to that website. And then I can run this tutorial. Okay. We'll come back to this. More Galaxy instances in a bit. So let's go here. Okay. I think we covered... Oh, we didn't get one thing, which is the chat. Okay. So most Galaxy training network pages have this at the bottom, open chat. And if we click on that, it opens up a chat room. And most of these will link to the general chat room for the Galaxy training network. So if you have a question and it's not working for you, or you want to know how to do things, or if you want to even know how to write a tutorial, you can click on that and you can start chatting. This is implemented in Gitter. And you can go there by clicking on that, or you can just start chatting here. Okay. So I'm going to close that. Really, if you have any troubles, I can't recommend that enough. This is me going to chat and actually asking questions of people. And there are people on all day around the world. So let's see. Okay. So learning to walk. So if you're new to Galaxy, those are resources I think you should spend time with. So the training network and focus on the introductory material. This will get you introduced to how things work, what the terminology is, what's a history when we talk about histories? What do we mean by dataset? What the three panel interfaces, how to run a tool, how to connect tools, things like that. So now that you have the basics of how to use Galaxy, you probably want to use Galaxy for your own analysis to ask your own questions. And this is going to require a bit more knowledge than what you got in the learning to crawl. So that's what this section is about. Okay. This is much longer than the previous section. But first question is where to run your analysis? And we saw in the training network that it gave you some options there for where to run the tutorials. But it turns out there are a lot more. So I'm going to click on that. Go to the Galaxy platform directory. And let's zoom in on that. One more. Yeah. Okay. So as I mentioned, it's open source software installed in hundreds, not thousands of places. If it's publicly accessible, we list it in this directory. Okay. And right now I'm going to focus on two of these. So we classify the public servers according to if they're use galaxy.star servers or public, or they're available on clouds or containers or VMs. Right now, let's focus on these two, use Galaxy and public servers. This is the use galaxy.star alliance. And these three servers are great places to start. So if you don't know where to go, start with one of these, you won't go wrong. They are all backed by large groups in each of the three continents. This one's based in North America, Europe and Australia. They have lots of compute resources, lots of storage. I think they all have storage limits of 250 gigabytes per count. So there are limits, but they're pretty robust. Okay. Next thing is public servers. This lists every public server you can just go to on the web and start using. Okay. This includes the use galaxy.star servers. And there are currently 134 of these. Okay. And they run the whole range. So there are some that are very specific for very particular problems. So this is about genomic variations. This is for marine science, although it's pretty generic. If you see genomics over here, it means it will have a lot of tools that you can use on it. This one is about age-seq, a particular method. Okay. So if you're doing age-seq, that's a great one to go. If you're doing something pretty generic like RNA-seq and differential gene expression, there's going to be a lot of choices for that. If you're doing something with antibiotic resistance, ARGS OAP is hugely popular. There's also RGA. There are a couple others. Areas is about public health. Turns out we actually have a lot of presence in public health. I didn't realize that until recently. But you can go through a lot of these if you're doing something very specific, like molecular dynamics and free energies, you probably want to use bridge. Okay. But something more generic, you're going to have a lot of choices. You can search here as well for what's going on. If I search for molecular, there we go, molecular. I've got this, that chemical toolbox. So if I'm doing something like this, I may actually have a couple of options. Okay. So that's the public server directory. A couple of things. If I scroll down, which platform type to use, and then it shows us our six categories, use Galaxy Public Servers plus these. Right now, let's focus on these two. And in particular, I want to focus on this one. And we will come back to this. If you have absolute data security requirements, meaning maybe you have data with personally identifiable information in it. So it's about people. You should probably not upload that to our public servers. And in fact, there's a warning at the bottom of all the used galaxies anyway that says don't do clinical here. Okay. So they are as secure as we can make them. But still, you don't want to upload that data to a public resource. So if this describes you, we will come back to this and it will be over here in this area, the local, the commercial and the academic class. Okay. So that's the first thing is where can you run it? Okay. And again, lots of choices at all at that URL, the platform directly, galaxyproject.org slash use. Okay. When you did the intro, you will have learned the basics, but each of those has more to it than you will learn in the intro. And I really want to recommend these three tutorials. So all these three link out to tutorials in GTN. Yes, so getting data in a galaxy, it turns out there's a lot of ways you can use FTP. And as you scale up what you're doing, you're going to want to know the other ways to do it. Because what works well for doing two or three or five datasets is not going to be so good for doing 10 or 50. Okay. So getting data in a galaxy, understanding the history system becomes important as you get more than one history. And as your history start to get longer. So history is an analysis that you've done or are doing. And again, when you start out, you can just do it. But as you go and you get longer, you're going to want to know the tips and tricks. So how galaxy supports bigger histories and more of them. Finally, you also want to know downloading and deleting data in galaxy. So you can get your results out, downloading and deleting because you may want to because you may run out of your allocation. So these are very important as well. So something about this slide is I'm not going to visit any three of these tutorials today. They're just links. And as we go, I'm going to do more and more of that just have links that point you to things instead of exploring them. And that's because of time. So, okay. So that's that now. If you've done all that, then you'll have a pretty good idea of how to use galaxy in a way that will let you scale up to do more histories, longer analysis. But you actually want to do it with a particular domain. And so let's follow this up. So if you have it, if you are using a galaxy server that is on a relatively recent version, it's going to have this. So let's do that. Let's take a look at use galaxy.org, which is the use galaxy North America. And I'm going to zoom in a little one, two, three, let's do four. Okay. So this is use galaxy.org. And it's sure enough, it has this. So I'm going to argue that when you are trying to learn how to do your domain analysis, I've got epigenetics I'm trying to do for transcriptomics or you name it any number of topics that the galaxy training network, which I'm going to bring up over and over today is a great place to start. And you can get to it how we got there before, which is training.galaxyproject.org, or you can also, excuse me, get to it from this. And there are reasons for doing this. And let's, you know, let's actually highlight that. So you're on your server and you see this and you click on that. And it doesn't take you there necessarily as it so much as it presents a window within a window here. And so this is the same website we were looking at before. And let's see, there's all sorts of different areas here. So computational chemistry on bridge, I think there's a tutorial there for bridge climate. This is a really valuable point about galaxy that I should make is galaxy was created for biology for life sciences, but it's actually a domain agnostic platform. And it's been applied to domains outside of biology as well, including climate. So computational chemistry sort of related to biology, you know, at least more so than climate. But a whole bunch of things here that you can do tutorials about genome annotation, if you're doing metagenomics, I recommend taking a look at these tutorials and say, okay, introduction of metagenomics, how to do 16 s stuff in here about mother, okay, good, things like that. So if you are doing a domain question, and we have tutorials, I recommend looking at them if you're working in proteomics, take a look at that, you may learn there are tools and resources you didn't know about. But let's come back to, yeah, so we got to this by clicking on this. I'm going to go into introduction, same place we were before. This is the same tutorial we were in before. Okay, short introduction to galaxy by Anna, again, and I scroll down. And maybe I'm running this one. Okay, although it has nothing to do with domains. And I get down here and I get to the fast QC. Now you may or may not recall before, when we were here, that this was not a link. And now it is. And what this means is that since, well, since I invoked the GTN site from within a galaxy server, the GTN site knows that. And it translates these toolboxes from just being tool text boxes into links. And so here it says what you want to do is type fast you see in the tools box, and you want to do this, that and the other thing. And then you want to click execute. Since I came here from here, I can now do this. I'm going to click on that. It takes me back to the server that invoked it. And it takes me to the fast QC tool. Okay, and maybe I forgot what it told me. So I go here. And I go, okay, I want to do okay, good. And so I do that. And then I type that in, you know, whatever it told me to do. Okay. So the GTN is integrated with galaxy. And it's not to a particular galaxy. Here I'm using usegalaxy.org. But it knows which, which platform called it. Okay. So really recommend this. If you're learning, it's a much less frustrating way to do things. And it can take you back and forth. Okay. Okay. So let's see. I recommend the GTN for learning how to use galaxy and for at least looking at some, some domain specific training. So epigenetics, transcriptomics, whatever you're doing, take a look, it'll give you an idea of how to do it in galaxy. Okay. It may be that if you're already, excuse me, it may be that if you are, are already an expert on your domain, you don't need to do that.  You're just using galaxy for its reproducibility and storage capability. Okay. That's fine too. Okay. So I did that. Okay. So get your domain knowledge from the training network. No, you can get your domain knowledge, get your knowledge about that domain and galaxy from the training network is what I should say. Okay. Next thing in learning to walk, there are a whole bunch of other resources that, that can help you get your question answered to run your analysis. And that's what this is about. So something I want to talk about is tools. That's what you do in galaxy is you run tools. Let's see. And this is fast. Yeah, let's do fast QC. Where am I right there? Okay. So you run tools and you find it by, you know, yeah, you find it over here, fast QC, which is going to be in the short reads data quality show sections. There we go. Quality control, fast QC. It's right there. Click on that. So in galaxy, you're going to run a lot of tools and you may have questions about those tools. I don't know what the contaminant list is. Okay, well, maybe you don't. There's some help right here. Okay, usually tells you, okay, what it's expecting. That's cool. Okay, what if there's not or what if that's not enough information, you can scroll down and let's see here, there's documentation about each tool at the bottom of everybody's tool panel. And it says, okay, here's what we're about. Here's where we are described some inputs and outputs. Okay, so you may be able to answer your question by scrolling down to the bottom. Finally, if that's not enough, there's often a link out to documentation. And here there is. Okay. And if you actually want to understand the that's not there. Okay, if you actually want to understand what the box plot that comes out of fast QC means, this is actually great this documentation. And so if you follow this out, you can find out, okay, what does that box plot mean? Fast QC has very good documentation. So this is highly recommended. Sometimes that doesn't exist. Okay, and what happens if you can't find that? Let's talk about that next. Oops, wrong one. There we go. So yeah, go to the bottom. Okay. So most tools like fast QC, they weren't written specifically for Galaxy. They are general purpose tools. They're available outside of Galaxy. That means that they have help information typically on the web. That's outside of Galaxy as well. So if you have a question about a tool, things like Google and Duck Duck Go are your friend and really search the web. There's also two websites I want to highlight in particular the Stack Exchange Bioinformatics site and Biostar because they are question and answer forums online where you can ask questions about bioinformatics. Okay. So if you can't find information about your tool that you have a question on through Google, you can ask it there or there. Okay. What if you aren't sure if your question is about a tool or if it's about Galaxy itself or you're pretty sure it's about Galaxy itself? Then you have a whole bunch of options. And let's explore those. So pan galactic search here. Let's go there. So maybe you have a question about Galaxy. This is the, excuse me, the Galaxy Community Hub at galaxyproject.org. This is at the top of every page. And I'm going to search for my, what happened to my history? Or my history disappeared? I don't know. My history disappeared. So maybe your history, that was good. Okay. So maybe your whole history has disappeared and like, I don't know why. And so you come here and you type this in. And what this does is this uses Google, but it uses a custom Google search to search only sites about our Galaxy. You're not going to get results about football teams or about cars or chocolate bars or astronomy or phones. You're going to get results about the Galaxy Bioinformatics platform. And so here we go. It came out pretty good hits here. This is on BioStar, which we used to use. So that one's pretty old. My data has disappeared. History completely disappeared. Okay. That's probably what we want. Okay. So our second hit is good. Missing history. Okay. Support missing history. Okay. So let's go to this one because I think that's our next item.  And then we go there. Okay. This is the Galaxy Help site. And it's an online forum for asking questions and getting answers. And here someone has said, history completely disappeared from local Galaxy after relaunching the instance. Okay. This may not be exactly what my question was because I was probably on a public server, but there's actually a lot of stuff here. Zoom in. Okay. And I could search here for history. I don't know if that works. History and data disappear. Maybe it's here. Okay. But there's actually an awful lot of help here. And you can ask questions here or you can find out who else has asked questions. And if no one has asked your question before, you can sign up and post your question. Okay. So that's a really great resource. So those are the first two, I think. Yep. Angolactic Search. Start there. Helpgalaxyproject.org to help website. We were just at. There's also Gitter Chat. Okay. So if we come back, where's it right here? Okay. Nope. That's not it. Okay. Again, back on the hub, Galaxy Community Hub, every page has this on it. And you can click on that and it will take you to Gitter. But a different Gitter channel than you get to in the Galaxy Training Network. This is a general Galaxy help. Okay. And you can ask questions there. Okay. So there's Chat as well. Galaxy Fact Page. That's an excellent resource. Let's go there. This is maintained by Jen Jackson, who leads our support. And using Galaxy Basic Facts and scroll down here. Unexpected results. My history is missing. There we go. It shows up again. Good news. It probably isn't. So that's a page that directly addresses my question. Okay. So lots of great resources there. Videos. Let's talk about videos. Galaxy has a lot of them. And we have a YouTube channel that manages the recent ones. And there's two I want to highlight in particular for this. And one is this collection. Get started.  It didn't help. Okay. It's a whole bunch of videos. It looks like 16 videos. And I think they're all very short. And they talk about various... Stop. Okay. We're going to talk about various aspects of Galaxy. So what is Galaxy? How to get your data in uploading by FTP. Very helpful short videos. Okay. Quick tips are longer than these. But again, same sort of thing. Lots and lots of helpful stuff. The other thing I want to highlight is training. So if you go to training, we have lots and lots of videos about training. Now, these are different or distinct from the ones we saw within the Galaxy training network. These were created for training events. Like the one that's going on right now, plant transcriptomic analysis using Galaxy. And their videos created for training events. And there are lots and lots and lots of these. Okay. And so if your topic of interest has been presented in the last year, there's going to be a video here about it. And it covers the whole thing from intro to Galaxy to machine learning with Galaxy. How to do all sorts of things. So these are really valuable resources. You can play them. It's just like being in the training. Can't recommend those enough. So, okay. Got both of those. Okay. Other community resources. The Galaxy event horizon. This is a webpage that lists all upcoming events that we know about. There's a few things I want to highlight. One is the 2021 Galaxy Community Conference being held online. It was going to be in Ghent, Belgium, which is lovely, but we won't be there. We'll be online. 28th of June through the 10th of July, this starts with a week of training on the 28th of June. And then we have a three-day meeting this week and a two-day collaboration fest. And it's really affordable. Really, really affordable. So if you want to learn a whole bunch more about data intensive research and how to do it with Galaxy, this is the place to be. Another webpage is the webinars. It'll list this one plus the upcoming three plus a couple more. There's another one this week by a group in Norway that set up their own Galaxy server for using machine learning and immunology. So that's, I think, tomorrow. There's a news page, including a monthly newsletter. It lists all sorts of upcoming stuff in the Galaxy. We have blog posts from all over the web that we collect. We have a communities page that lists things like Galaxy Australia. It does not yet list things like the public health community. I will get to that hopefully in this month. We also have mailing lists. These are not as popular as they used to be. We've moved to forums and chat. But we still use them. We have an announced mailing list that the monthly newsletters go to. And there's a couple other lists as well. We're also on Twitter. And Galaxy is really on Twitter. We have a lot of accounts. Everything uses the hashtag use Galaxy. And there's a link to those postings. So if you want to keep up, I recommend that. At least following this hashtag, maybe following this or this and or this or the UK fee. There's a whole bunch of ones. The last one is the Galaxy community hub. I am actually going to go there. Let's go there. Okay. And this is the hub for the community. There's lots and lots of resources here. If you scroll down on the landing page, there's links out to everything I just talked about. The news feed, the newsletters, the events page, the Twitter videos, blogs, careers. There's a lot of demand for people who understand Galaxy. So we have a careers page as well. And then platform news as well as some recent hubs. If you go up here, community events, news blogs, same sort of thing. Okay. So lots and lots of stuff is there. Okay. How are we doing? Okay. Let's do that. Present. Okay. Going the wrong place. Okay. So that's it for learning how to walk. So we went through learning to crawl the very basics concepts. What's the history to learning to walk, which is how to do your domain, how to do more sophisticated histories, more sophisticated analyses, how to find out what's going on, community resources. But now our last topic is learning to run, becoming a Galaxy Power user. So as you scale up what you're doing, you're going to need the features that were introduced by the stuff in learning to walk. But if you start to get bigger, if you start doing stuff with 100 data sets, for instance, you know, maybe paired in reads times 50, and maybe 10 individuals over five time points. Okay. Those methods are still going to work, but they're going to become tedious unless you start using the Galaxy features that support large experiments. So that's what this section is about. Okay. How to really scale Galaxy up to support large experiments. Okay. Which it does, but not through the ways you learned in the first two parts. Okay. So the first thing I want to talk about is what if I want to rerun my same analysis over and over? Maybe you've got an experiment and you get new data every Monday morning. And you don't want to have to type in and click and point and select the same thing every Monday morning. What you want to do then is use workflows. Okay. And workflows are repeatable recipes in Galaxy. And what that means is instead of redoing everything Monday morning, on Monday morning, you upload your data.  And then it goes off and it reruns your analysis on new data every Monday morning. So that's workflows. There are two tutorials that cover workflows in the basics. Extracting workflows from a history tutorial. So you can actually create an analysis and then say from the analysis I just ran, please create a repeatable recipe or workflow. That's what that's about. And this also talks about the same thing. Okay. But workflows are way more than this. They have a whole bunch of features. This is a video from a webinar maybe a month ago, maybe a little over a month ago. And it is a tour de force of workflow features. And if you're repeating your work, you should absolutely watch this because it talks about all sorts of cool ways to use workflows, workflow features for how you change parameters each time you use it, checking for best practices. It's an amazing video. So really can't recommend that enough. Do you want to learn more about creating and editing and importing Galaxy workflows? I recommend this tutorial. It turns out you can create them from scratch as well. And then once they're created, you can edit them and change them. Important Galaxy workflows, you can create a workflow on one server and then import it into another. And as I mentioned, they're also parameters and there's a tutorial about that. So really, if you're doing something more than once, absolutely you have to look into workflows. Okay. What if you are starting to do complex slash large experiments? Okay. So, you know, the methods you've learned already might scale, well, certainly scale to five, maybe 10 data sets. Not going to scale to 100. What do you do if you have 100 data sets you're operating on and you actually have a complex experiment, like 10 individuals at five different time points? Okay. In this case, you're going to want to spend some time learning the rule-based uploader, the collections and tags. Okay. And here are resources for learning all of those things. Briefly, rule-based uploader says when you bring data into Galaxy, you can actually use the information in the files and the names of the files to pre-group them into collections. And what are collections? Collections are semantic relationships between files. So in the intro and even some of the medium tutorials, you may not be using collections. Every data set is an individual data set that works fine when you have five or 10. When you have 100, it's going to drive you crazy unless you start using collections. And it goes everywhere from the bare minimum, which is I have a paired end data set, here's the forward, here's the reverse reads, put them together in a collection. Two, I have a collection of 10 individuals over five time points. Each of those have two files. So it can really be arbitrarily complex structures and you can create these semantic relationships. So Galaxy then knows that those files are related. You can then run tools on collections. So you don't have to run a tool 50 times. If you have 50 pairs of forward and reverse reads, you can run a tool once and say, please run it on this collection. Behind the scenes, it will run it 50 times. But in the interface, it doesn't have to do that. Tags are a way to keep track of where things came from in the longer histories. So once you get past 10 or 20 steps, it's really hard to keep track of where things came from. I really recommend using tags once, well, once you get past 10 steps. So really spend some time learning these things. It will make your experience much more efficient and rewarding.  It turns out that you can also access Galaxy programmatically through Python. There's a BioBlend library, that's what we call it, documentation. And it talks about how to actually access Galaxy. So if we go back to our Monday morning example, instead of you manually starting that workflow every Monday morning, you could have a script that runs every Monday morning, checks the directory, if the data is there, it then invokes Galaxy, logs in, transfers the data from that directory into Galaxy, and then launches the workflow. And so you don't even have to do anything every Monday morning, it's just all automatically set up. And what you get for that compared to just using your own scripts is you get everything that Galaxy offers. You get the user verification, the authentication, you get its sharing capabilities, and you get its reproducibility. Okay. So there's also a PHP library for doing this, this is used by triple, if you're familiar with that. So what if you need more power? How am I doing for time? Okay, what if you need just more compute power, more storage than you can get on any of these public resources? So you remember this from early in the talk, we talked about these two. You can use academic or commercial clouds, or you can do a local installation. So let's talk about some of these. This is the academic clouds tab that you'll see on the platform directory. And let's see, what is it good? It's okay. Yeah. So it turns out that Galaxy has been ported, or there are ready to run Galaxy instances on a lot of academic clouds. And this is, excuse me, this just shows the first five, but it's a pretty good sampling. So let's talk about them. Let's start with Climb and GenAP and Jetstream. These are all national cloud infrastructures. This is in the UK, this is in Canada, this is in the US. There are also infrastructures in lots of other countries like Italy and Norway, Czech Republic, you name it. A lot of places have these and they have ready to run. You can launch your very own Galaxy instances on the academic cloud platforms in those countries. So that's what these three are. Anvil, I'm really glad this isn't the top. So this is funded by NHGRI or NIH in the US. And it is a FISMA moderate environment. So it's not CLIA certified or HIPAA certified, those are terms used in the US for clinical stuff. But it is a secure environment. And so you can actually launch your own Galaxy in Anvil and it runs in a FISMA moderate environment. So it's not quite clinical, but it's a step in the right direction. And this one actually integrates with a whole bunch of controlled access data sets at NIH. And it's kind of cool. So the advantage of these cloud instances is you can often scale them up arbitrarily. And that is certainly true of the commercial clouds. But you need to get an allocation. You can get an allocation from Jetstream or GenAP or Anvil or Climb. And then you can spin up your instances. You can run your analyses. You can export your data sets.  It's a very cool thing. What if that doesn't work for you? You can set up your own Galaxy instance. Why might you want to do this? Security is a big one. Okay. Oops. That's not what I wanted. Security is a big one because then you can put it behind your firewall. Galaxy is used in lots of research hospitals. And it's behind their firewalls. You get to set your compute and storage limits by what you have available. I don't get to set it. You get to. You can also customize your server. So you get to control the tools and a whole bunch of other things that are on that server. But the resources for doing this are outside the scope of this webinar. So please join us on May 26th for I think that's the last one in the series, which is Galaxy Resources for Administrators and Infrastructure Providers. Okay. How am I doing? Oh, okay. I went over one minute from what I wanted to. Thank you. Thank you for listening for the past hour. I really want to thank the Galaxy community. And this is literally thousands of people who have contributed tools, documentation, support, training, resources, code, testing. You name it over the past 15 years. And NHGRI, NIH, and NSF are the three U.S. agencies that have funded me personally over recent years. So I'd like to thank them and you guys for sitting through this and questions and answers now, please. Okay. Thanks to you also, Dave. Yeah. I hope our audience found the talk useful today. There are a couple of questions and we have like eight minutes or so. So I will read them for you. Tatiana asked if you can use usegalaxy.org.au if you are in the U.S. Yeah. Okay. I just stopped sharing. Good. Okay. I thought maybe I stopped recording. Yes. So all of the usegalaxy.stars, at least those three, are accessible to anyone in the world. So, yes, you can use those. Some infrastructures, so usegalaxy.no for Norway is only accessible to Norwegian researchers. But if it's listed in the usegalaxy tab, then anybody can use them. Okay. And we encourage you to do that. Okay. Thanks. There is another one that is somehow related to this. Can you have accounts in multiple servers or move your analysis from one server to another? Yes. You can have multiple accounts in, excuse me, in different servers and that is not considered abuse. Okay. So it's possible to have an account on all 123 of those public servers, 134 of those public servers. And they each are independent. So they don't know about each other. So if you create an account in one, it has no impact on another server. Now, that's good and bad. Okay. It's mostly bad because what we want to have is a federation of galaxy servers where if you run something on this server, that's great. But it doesn't have this tool that you want. It's over on this server. Long-term, our hope is to provide a federation of galaxy servers so that you can move seamlessly from one to the other. We don't have that yet. And I don't think that's anywhere close to funded yet. But that's our goal is to have a federated galaxy where you can just move around. As far as moving analyses from one server to another, yes, you can. You can export either a dataset or a complete history from any given instance. Okay. You can also export a workflow from one instance and then load it into another. Okay. So, yeah, you can move things around. I have never tried importing a history. So I don't know if that works or not. But I know you can export it. So you can actually get all of your datasets downloaded locally to your laptop. And you can do that with workflows. So does anybody know if you can upload a history? Yes. Yes, you can. Okay, good. Never done that. Okay. Okay. That is another question, I think, that it's just new. Can we run NFCore pipelines? Oh, man. Okay. And I'm not the one to answer that because I don't know squat about NFCore besides that is next flow. I can tell you that there is limit support for CWL in Galaxy. And I think next flow supports CWL. So a very tentative answer here. If you can get it into CWL, then I'm pretty sure you can get it into Galaxy and it might actually work. I know this is an active area of research to better support CWL in Galaxy. So not the most solid answer. Just another one now. Where can I find more information to troubleshoot a Galaxy 2 that is not working as expected? Okay. So if it's a specific tool and you're trying to use it, I would go back to the resources I pointed out. Think about using Google because it probably was not written for Galaxy. It's probably a generic tool, probably used by researchers around the world. And they may have a help forum about that. They may have a mailing list. They may have, you know, great documentation. They may not. Some tools have terrible documentation. But start there. Okay. If that doesn't bring you joy, there's the Biostart forum, there's Stack Exchange, the Bioinformatics Stack Exchange. If that doesn't bring you joy, you can go to the Galaxy Help Forum and ask the question as well. And again, sometimes it's not clear if it's a tool question or a Galaxy question. If that's, you know, like, where's the problem here? Is it that I'm not understanding the tool or is it somehow and how it's interfacing with Galaxy? In which case you want to start with the Galaxy Help Forum or the chat. And it's okay to start there too, if you're just not sure. Because it's a very friendly community. I feel like, I don't know, does that answer your question? Does that help at all? No. There is a reply to that. Try to Google and Galaxy Help Forum, but do not bring me joy. Okay. Then you need to ask the question. Okay. So formulate your question. If it's really short, you can do it on chat. If it takes more, then you want to do it in help. And just ask the question. And worst case, someone else has already asked it and someone will say, hey, take a look at this answer. But if you couldn't find it, probably it hasn't been asked. In that case, it's the best case because now we're going to have that question. Everybody can find it and everybody will be able to see the answers to that, the responses. Okay. Hopefully that will bring you joy. There is no reply. So let's assume this is joy. Another question. Is there enough tools to perform single cell RNA-seq analysis in Galaxy? Yes. I don't do single cell research, but there are whole servers that apply for single cell. The European group has a single cell server and the human cell atlas has a lot of single cell stuff as well. And they have a Galaxy instance. But in general, let's see, the usegalaxy.orgs will also have a lot of single cell tools. And I recommend starting with the GTN tutorials about single cell, because there are a lot of those as well. So two years ago, we were not so good with single cell, but now we're all over, in my opinion. Okay. Thanks, Dave. I don't see more questions, but I may have one for you. So you've mentioned different scientific areas and showed them in the GTN at all. So if you are a researcher and the tool that you are using usually is not available in Galaxy, how do you proceed there? Let's see. So if you want to use it in Galaxy, someone is going to need to wrap it. And wrapping a tool is the topic of not the next webinar, but the webinar after that, I believe. So in three weeks time, three weeks, yeah, in three weeks time, that's the topic of the next webinar is how to get tools into Galaxy, how to make your tools accessible through Galaxy. But if you're not the kind of person who knows XML or knows Python, then what do you do if you have a tool that you want imported into Galaxy and it's not currently supported? Well, a couple of things. First, you can check in the Galaxy tool shed. If you Google that, it will actually find our tool shed. That has 8,000 tools in it. So there have been a lot of tools that have already been wrapped for Galaxy. It may be possible then for you to write to a system admin on whatever server you use and say, hey, can I get this tool installed? I was looking for Diff the other day on an Anvil instance, and it wasn't there, but I went to the Galaxy tool shed and it was there. Since it's Anvil, it's a cloud instance, I'm the admin, I can customize it. So I installed Diff on my server. So that's one thing you can do is search the Galaxy tool shed. If it's there, you can ask your admin. If you're using a cloud instance, you can install it yourself because you control that. If it's not there, you have to find someone to wrap it or suggest that it be wrapped. Then it becomes a marketing issue where you might want to put it on the forum like, hey, I really want tool Diff. And if no one's wrapped it yet, then it's like, okay, you can also write to the IUC channel, the chat channel and say, I want this. The IUC stands for the Intergalactic Utilities Commission, which is the group that manages tools overall. So they have a good or chat channel, you can write to them and say, it'd be really helpful to have X. Long answer. Yeah, but a good one. Thank you. Okay, I don't think we have more questions and we are at the top of the hour. Next week, we have Saskia Hilton and colleagues, leading the educators and trainers. So if you are interested, I will share the link now in the chat. And you can register like you did for this one. It's at the same time. And I think that's it for today, Dave. Do you want to add something else? Thank you all. So thanks for your time. Thanks for joining. I hope that was useful. See you next week. Bye-bye.


to the speed of light, a cloud of dust and a hearty high old silver, the lone ranger. With his faithful Indian companion Tonto, the masked rider of the planes led the fight for law and order in the early days of the Western United States. His strength and courage were always on the side of right against might. No man could match his daring and resourcefulness, and nowhere in the pages of history can one find a greater champion of justice. Return with us now to those thrilling days when the West was young and the lone ranger was fighting the greatest battle of his entire career. Tiles to the south of Spanish flats and close to the treacherous Rio Grande, a small group of vicious outlaws met in a rocky glade. Each one was a leader in his own right, and when all had arrived and tethered their horses, close by a slim man with muscles of lip cord stepped into the center of the group. He paused the moment for attention, then spoke to the others in a voice as cold as chilled steel. Well, I see that Steph got in touch with all of us. All of us that are left alive anyhow. Then maybe he told you the big boss that I was to take charge of this, to give all the orders. Anybody got any objections? You buzzered? I reckon you're the man with his job, dirt. Nolan? All right with me? Yeah, don't nobody keep still now and kick later. If any of you don't like the notion of taking my orders, speak up now with his time. Well, then that's that. Now to get on with it. There's three fellas. Maybe a fourth if it's true we still live in. We've been raising pain with us. The three we know about for sure are Graham. That's the Omri that owns that big ranch. Arizona Lawson, the gent with a dog that hunts wolves, and Tauno the Redskins. Tauno with a Lone Ranger sidekick. I guess you've all heard of that, Omri. Well, the masked man's the fourth. Gunner was supposed to kill him. But lately I've heard there's a chance that he's still living. Just before the Hung Mendoza, he swarping down it was the Lone Ranger tricked him into attacking Laramie when Laramie and his gang had the sheriff corner. Yeah, I know. Two months ago at a meeting like this, boys, it would have been a dozen of us. But now, look, Gunner's gone. Turk Rigby. Red Camp. Laramie and Mendoza. Boys, that ain't the worst. Two months ago we had this part of the country in the hollow of our hand. There wasn't nobody. There wasn't nobody inside the lower out there to open his mouth again. Except in Graham, not even the sheriff. But now, because we've had some tough look, when they're feeling their oats and they're raring to go, boys were sittin' on blastin' powdery. With a short few. Yeah, but there's just one answer to that, fellas. Either we get things under control again or we're through. What's the big boss say?  By striking hard, fast and often. I mean that until things are back like they was, we're letting everything else go. Now each one of us has been after all the cash you can get. It'll come second. Some of us have had one kind of work to do. Some of us another. But now we all got just the same job. And after... Round up your men. See to your horses and guns. Startin' tomorrow we smash them. Raise. Burn. Let the coyotes the glory. We're fightin' our way back on top. They come doin' the night. They never give us no warnin'. Busted in the door. Shot down Sam when he weren't even armed. You ought to blame for that, Mr. Graham. Why couldn't you leave good enough alone? Why'd you have to call alone, the angel in Toronto and Arizona lost in here? If you hadn't done that, them outlaws would have left us alone. Mrs. Larson, please sit down. No, I won't. I won't. I won't. Mrs. Larson, please sit down. I won't sit down in your house. If I had an overall. Ain't I gonna write with my husband killin' my home burnt down? Isn't that all the more reason for me to continue the work I've started? Would you want me to stop while killers and thieves can still do such things around Spanish class? We were so happy. You still have your son? I had two boys once. There, you see? Wasn't it Gunner who murdered Ted? Yes. But that was before I even started this fight. Doesn't that prove you can't be short of life even if you do sit back and let these thieves have their own way? Men, good men, honest men lost their lives. Lost their lives when not a one of us had the courage to fight for decency and law. But the things those cooks have done these last two days, well, there's never been anything like it. Because they're desperate. They know they're being defeated. We wasn't the only ones burnt out. I could name you a dozen. I know, I've heard. No, Sam was the only one shot. You've seen the end of this. If anything, conditions will get worse before they're better again. But we must recognize that fact and face it. My friends and I have made a good start, Mrs. Larson. It'd be a shame to stop now. Won't nobody help you? You had almost no help anyhow. You almost had them fooled. You almost had folks thinking they could battle them outlaws and not make them suffer for it, but they won't be fooled like that again. You've talked to the people around? I have. Then I'm sorry. But it doesn't alter things. Ponto and Arizona and I hope to finally meet arouse all of you to the point where you'd have the courage to fight for yourself. But if you haven't, well, you'll just have to carry on alone. Mr. Graham. Yes? Ted's gone. And Sam's gone. All that's left to me now is my boyfriend. A good boy, too. But I warn you, it's only a few minutes on account of this war you've started. I'll see that you're punished if it's the last thing I do. If anything does happen to Frank, the blame will not be ours. But on the outlaws who made this war unnecessary. Well, that's what I come to say and you needn't think I don't mean it. Very well. What's the idea? What's this, Arizona? Graham, we found this Omri hiding a straw stack at the place where he'd work had been burned down. But you heard the crooks had done it with all our plans. We wouldn't tell us nothing until we'd seen you. Mr., you're here. Now you'd better open up before I go to work on you. I ain't telling nothing until I know I ain't gonna be harmed by it. You can't make me tell. What is it, man? Out with us. You promise they won't get me, Mr. Graham? You'll give me your word? No one will touch you. If you know anything, tell us. Tonight? Just about sundown. I'm gonna read Mr. Mander's place. Oh, poor man. You know that for a fact. Here's the truth, Mr. Graham. I swear it is. I heard them crooks talking. They didn't know I was anywhere's round. And you couldn't tell Arizona or Ponto.  I never heard of them till just lately. How'd I know they wouldn't get me in trouble for it? You waited so long we've hardly got time to get there. But I just... Ponto, run out to the bunkhouse and get all my men. Tell them to saddle and bring their arms. And tell the sheriff. Tell him if he wants to help, fine. But if he won't, he can go to Thunder.  You'd better come too. No, sir. I've done too much already.  Stay here then. It doesn't matter. We need men. Get to the saddle. We're riding. That man is placed below us. He's not got here yet. No need to go down there, though. We wouldn't get any better reception than anywhere else. Spread out and take the cover. Those coyotes show up, wait for my signal. Then let them have it.  Ponto, you stay with me. We'll get behind that ridge. Come on. Get him up there. Get him up there. Ponto, you stay with me. We'll get behind that ridge. Get him up there. Wake long time. Then not come. I can't understand it. I think they could have seen us ride up, Ponto. Not now. Almost two hours past sundown now. I don't like to think we're just wasting our time. That's right. On the other hand, if we left and they showed up afterwards, we'd never get through blaming ourselves. Ah. Wait. What matter? Listen, that might be them now. Well, that's just one horse. Oh. Ride fast, doll. Wonder who the dickens it is. It's Arizona. You hear dolls? That wolf. Right. Arizona. Hi. This way. Over here. Where's the sheriff? What's up? Just a second.  Get up. Oh. Oh. Oh. Oh. Oh, there. Well, that's been it. There's trouble. Never saw the fellow before. He belonged to one of them gangs. What do you mean? They made sure we wouldn't be nowhere near Spanish flat. And then the whole bunch hit that town and half tore to pieces. That's bad. Without the lone ranger to do our thinking, I reckon we ain't worth the powder to blow us out of our sand. Marlon Ranger story. Before the next exciting scene, please permit us to pause for just a few moments. Ben Graham, his crew, Tato and Arizona return to Graham's ranch house at same night. And it seems a number half the residents are Spanish flats. Only the threat of gunplay finally persuades the crowd to disperse. Get out and keep out.  I'll order my men to fire. Where are you going? I told you to forget what we told you. Oh, this is you fellas' fault. Come on inside, Arizona. You too, Tato. Why can't they see what we're doing is for their own good. You men are accepting all the danger and you haven't even a stake in the district. That's all the way, Graham. Tato and me didn't get into this fight looking for gratitude. That's right. Frankly, I'm discouraged. You and me both. It's hard to know whether to go on or not. The three of us can do only so much. Four of us, you mean? You think the lone ranger is alive? Don't you have that mix-up down with the real? Well, I did think so then. What's changed your mind? I can't understand why he's living in contact with us. Certainly, what if he could? I've been wondering about the same thing.  I mean, I'll see him. There you are. And they've been closer than if they were brothers. I'm beginning to suspect the masked man who caused Laramie and Mendoza to fight wasn't the lone ranger at all. They fought over money, didn't they? Something about Laramie is supposed to send cash to Mendoza for one of their deals. And Mendoza not getting it. In other words, the whole thing could have been brought about if he had it to appropriate that money for himself. Yeah. So, you see, we have no reason for hope after all. And without the lone ranger, how can we go on when we have to fight not only those outlaws and the man behind them who does this scheming, but public opinion as well? Yes, Mendoza. A shout-out, though, but I'm going to be honest with you. I think... What was that? It's window. Graham, what you gonna do? He moves out there. Trying to find out. What? Hey. What was that thrown in, huh? Who's that? Who's that just running away, Graham? Can you see him? Hey! Hello there! Hello! Graham Moose. Well, whoever he was, looks as if he came here just to throw that stone for the window. Pick it up, Mendoza. It's not stone. It's bullet. What do you mean? It's bullet of silver. And here's his message. It wrapped round bullet. Bullet of silver? Why, that must mean... It's low-ranger. That must have been the mask man. I wrote away just now. I wouldn't answer when we called. Well, I'll be... What's that message say? Who called her name? Frank Larson. Frank Larson? His mother was here this afternoon. What did he do? He's the operator and agent at Roseville. And he's flat. It's not a town, really. Just a sighting where cattle are loaded occasionally. Why? What's Frank Larson and Roseville to do with that note? Yeah. You won't read it. Hi there, Wolf. Glad to see you back. That's a lot to know. Down boy. Down. What do I do? I kind of hate to pack it. Leave this place. It's the right good place to camp. Me know what me do. If you say so. Make a roll. High saddle. Then we leave. Dirk, what we didn't do to Spanish last night weren't worth doing. Yeah, that's right.  And when I think of Graham and Kato waiting at the manor's place half of the evening on my face, oh... Well, I'd have given them a meal and just to see their faces when they learned the truth. Honest, I'd have given two men. Well, you handled that all right. Didn't I, though? Yeah, so far everything we figured on the way it was playing. We got them turning yellow again, boys. Finish the job the way we started and they'll never make us trouble no more. You all right, Dirk? There comes the buzzard. Oh, hi. Let's see what he's got to say. Oh, hi. Good morning, Dirk. Good morning. Well, Dirk, I reckon our worries just about over. Yeah, what do you mean? Well, Graham's place, just like you said. Yeah, huh? First off, the folks in town were out there raising kings. Didn't leave, but Graham's crew showed their iron. Yeah. What else, then? Not as much as I stayed with the ranch house. Crews drifted along the bunk house after a while. Once I heard some fellow gallop off in the far side of the house, didn't get a good look at him. But that ain't what's important. Later on in town, oh, an Arizona came out. They cut over west and I followed. Yes, where they were going. Get on with it. They're camped. And guess what they done when they got there? Tell what you got to tell them, get it over with. They packed up and headed north.  They're pulling steaks. They're clearing out. How do you know? Ain't that doing a heap of guessing? Yeah, it would be if that was all I had to go on. But it ain't. I heard them talking for the road over. And they said to even the folks that were trying to help would turn again and they were through. Said they'd had enough. They're gone and they ain't coming back. Yeah. What do you think of that? You sure they said that? Buzzard, you sure you didn't hear it because you wanted to hear it? Think I'm a fool? Well, I just am. What do you say, Dirk? You've all heard the news. And I reckon that job is done. All right! Do you think I could dispatch the colon use then? Right now he's talking to the operator on down the line. What trains that outside? Ma, you can ask more questions in a room full of school minds. That ain't no plane. Just a switch engine. Yeah, I've been here almost a week now.  Amar, you ain't told me yet if they've caught any of them skunks of greater town. No. What can the law do? What can any of us do again outlaws like them? Got half a dozen gangs.  In fact, they'd rather and behind them making their plans and giving them their orders. That boss of theirs, nobody's ever been able to learn anything about. You know, son, the best thing to do is walk soft and try to keep them around in them. Oh, maybe. Oh, gosh. What time's that clock say? 11.30. Don't go near midnight. Come on, stand aside from that telegraph. We'll have to get a hold of the operator there so we fill the conductor to stop back by the side when they get here. Why, son? Because if the eastbound don't, it's going to go slap bagging at just about 50 dozen cattle cars. So I don't have to hurry that. Oh, what's that? I've got to send a message. I reckon not. But if I don't, there'll be a smash up. Yeah. I know. Eastbound now. I reckon that's what Dirk told you. That's the train. We're going to stand still until after it's hit. I think everything's all right, Nolan. Shouldn't Dirk have come around? He's keeping guard over young Larson at the station. How could he? Maybe he might bring the kid here. He's tearing that along. It won't be long now. The station's just the other side of that line of trees. We hear the crash plane. We wish we could see it. You'll stay under cover until we know nothing's gone wrong. He's passing. You can see her headlight. Get ready, fellas. Once she hits, we ride. It must be almost at the station now. What's wrong? Have you taken a long time? Wait. What we heard, a little flat-card, which engine got behind him and shoved him off the track into that draw. All right! Where'd they come from? We ain't got a chance. They're coming from everywhere. Oh, if you do, you pole-cap just try it and see what happens. No, shoot! No, shoot! Ah, darn you, buzzard! You said Arizona Tondo left the county. I heard him say that. Guess what we wanted to hear and say. I don't know you was trailing us.  Wait! You said it, fella. We ride. Hell-train crew. And they load up the eastbound with every tough army they had on the payroll. Well, that's your dirty double-crosser. I'll bet you worked with them.  You told them just what we was playing. No, I didn't. Honest fellas, I never said a word. If I thought you did, buzzard, I'd cut you down even if they got me right afterwards.   No, gents. What this fella you called buzzard gave your game away. Well, then who was it? It was a man you ain't never laid eyes on. Even though he was spying on your camp on your scheme to do this. What? He sent us your scheme on a bullet. A bullet? A bullet of silver. Silver? And if you want to know what to call him, just say The Lone Ranger. You've just heard of the copyrighted feature of The Lone Ranger Incorporated.


You know, it's freezing, I just got here, I'm going to do like light jumps, just take it easy, I'm really sore from my last workout, and just see what I can do, get some max jumps and probably lift explosively, but it's freezing, see what I can do, baby rim for technique as well. I have full aggression to go hard, it might be because it's slow, so I can take it slow, focus on my technique instead of going too hard, some decent jumps, I'll tell you more after, but last one. I'm feeling fatigued, but I got up a little bit, not my highest, but I'm happy with today's kind of exactly what I wanted to do, get some technique work where my slow approach jumps felt good. I felt more on point today, I focused on technique and taking it slow, so that's why my jumps look slow, but also because I'm really fatigued. I feel like I'm a little bit overworked, but I felt like that during the middle, but now I feel better, so I'm going to see how I feel in two days, I'm going to do a little lifting now, explosively, and then in two days if I feel really fatigued, I'm going to take it easy and just do recovery explosive stuff until I feel 100% because I don't want to overtrain, and I'm jumping good, my technique is good, so I think from now on, it's not from now on, but going forward, I'm going to see how I feel, take it easy, still focus on technique, keep coming back to this rim so I could focus on my technique more, it feels really good, and then dribble dunks felt really good, especially one hand, but my two hand was terrible, but it's not bad for being so fatigued because I feel really, really tired, it's really hard to go 100%, East Bay felt better, and I realized I need to go a little bit more to the top of the key, so that felt good, I got a couple of these in the tent, but still pretty bad, and all my motions are really slow on all my tricks, so footwork's feeling good, but technique on dunks is really slow, that's just fatigue, and my jumps feel slow, you can probably tell I'm not running full speed or anything like that, but it feels good to focus on technique, I feel like if I have another healthy day here I can really kill it, and then once my technique is on point here a lot, I'm gonna start moving up to other rims, because I was doing good on higher rims, but like always off and on, so I really want to get my technique down pat, keep lifting, I just got back into lifting, so that probably fatigued me because I lifted the day after that big session, so probably almost close to overwork, so now set this a million times, but take it easy, recover, and have another dunk session, let's go! It's freezing, so on days like this I like to do light dunks, a little less dunks and a little more workout, but I kind of got carried away with the dunks because it was fun, but I like to do the explosive lifts, a little bit of weight, but just do everything explosive, focus on going, what's up bro? We out here, so yeah that's pretty much it, just work, focus on the transition from the downward to up phase, and there's Skylard, very he is pulling up, saucy, wicked shots, and some saucy dunks, I gotta go, and some saucy dunks, saucy dunks, saucy, saucy, saucy dunks,


Earlier this month, I was given an exciting opportunity to play a Nintendo Switch game with a bunch of my friends before it even released on the console, and that game is LEGO Bros. LEGO Bros. is coming to Nintendo Switch, PlayStation 4, and 5, Xbox One, Series X, Steam, and Apple Arcade. They are graciously sponsoring this video, which means, yes, you can add collaborating with LEGO to the list of companies I've had the chance to work with on my YouTube channel. I'm so excited for you guys to watch the video and how much fun we had, and please, if you want to support me in cool collaborations like this, click the link in the description and check out the game LEGO Bros. coming to Switch and everywhere else. On with the video. I'm so excited, you guys. We've done this so many times, Miss Click. We have, we have. You're like my go-to bestie for Smash-type video games but themed something else, and this time it's LEGO, which I'm really excited for. We played with LEGO as kids, right? And stepped on many. Yeah, yeah, yeah, yeah, because, you know, we're just that age. But we actually have two heathens in the chat who are in their late teens, who probably don't even know what LEGO is, which is crazy. Scooty and Ham, you ever heard of LEGO? Would I'm 30. The multi-million dollar thing? Yes. I've heard of LEGO. Yes, I have, I have, I have a whole space shuttle discovery over there. Yeah, my mom actually recently did something really loving and threw away all my old LEGOs. Oh, that's so sweet of her. That's so sweet. I recently bought the Friends and the Seinfeld kits. Also, I have the Nintendo LEGO, but now I'm just showing off all my LEGO. Get excited, but I haven't made it. So it's just. I want to make that so bad. Needless to say, I'm a huge fan of LEGO. So when I heard about LEGO Brawls, I was like, you know what? I want to get all my buddies together, all my besties, and I want to brawl them in LEGO. And I make sure nobody starts the game yet because you actually get to design your own LEGO Brawler for this game, which I thought is what we did at the start, but I think we actually have to go through a tutorial first. Oh my God. I don't know if this game is exactly what I thought it was. Wait, is this a Boba? A what? A what? Like League of Legends. I heard Boba. Did you hear Boba? I heard Boba as well. Is this Boba? I was going to say like the drink, but we all hear what we want to hear would. I don't want to hear that on my family friendly channel. My Y button is very, is very sticky. There are reasons for that. You want to explain? Would stop. So while we're doing this tutorial real quickly, you should know Scoop by now fellow member of the Nintendo podcast, Claim to Fame. Right? You're in the episodes. I didn't know I was a member. Miss Click was also in an episode of the Nintendo podcast. And Ham refuses to hang out with me every time I'm in New York. He has some excuse. That's not true. You're wrong. You're wrong. I'm actually in New York on Saturday if you want to hang out. I'm going to be in LA. Right. Yeah. Okay. Busy. OK, I'm already customizing my character. It looks like we have to unlock a lot of stuff, actually. A lot. But I'm already liking what I'm making. Definitely want to keep that face. OK, when? Where did my hair go? Bring my hair back. We're all silently creating right now. I'm I'm really enjoying the cup. I know. I can't wait to see what you guys come up with. Oh, how did you? That was a cat, Miss Click. You're holding two fish. I got two fish. When they said that this was going to be a unique approach to the brawler genre focusing on good vibes, I didn't know they meant kitties with two fishies. Invite code. What is it again? G-I-C-C-G-O-X. Or if you say it phonetically, a giggox. Giggox. Sounds like a big. Wait, how do you guys have bunny costume stuff? I don't have bunny costume stuff. Wait, we got bunny? Who are we?  That's me. Scoot. It's Jurassic World. Oh my god. Chris Pratt, where are you at? Yo, yo. This is not what I was thinking. It's different to how I was thinking. Well, how did you get a dinosaur? You just summoned a dinosaur? Call me Chris, because I just pratted. Cut that, cut that, cut that. I'm in the Jurassic World bubble. How would you like to verse my mic like a horse? Who is that? A gear team? I don't know. It feels like I'm playing Battle Bots. Wait, are you my friend or no? I'm so confused. You're green. Who's in the lead? I am. Let's go. Are you really? Let's go, Scoot-ish. The Scoot-ish difference yet again. I got a carrot launcher. Oh, I also got a carrot launcher. I got a baguette. I got fish. Oh, you can select your broiler. Oh, I didn't do that last time. Now I mean, now I'm the me I made. Would I overwhelm you guys if I told you there were 77 trillion combination possibilities as you build the ultimate Lego minifig broiler? There's so many combinations, and yet mine was the perfect one. Oh, are you what? Goodbye. What? Oh, oh, I'm sorry. Wait, what? No, no, no, no. Just click where you're going. No, I don't know where I'm going. What is this? No, no, no, stop. I just zapped everyone. I want to win one, man. I never get to win in my own videos, I swear. Careful, Scoot. Careful, Scoot. Oh, how deterrent it is.  I like this game. It's fun. Yeah, it's fun. You want to try the cooperative mode? Let's do it. Guys, what should I order for dinner? I think you should pre-order Lego Brawlers using my link down below. Tasty. If only there was more modes. Oh, wait, there is. There's five super fun modes, including Control Point, Brawl Royal, Free for All Brawl, Collection and Party Mode. Chris Pratt is on my screen right now. Lego Chris Pratt. Dude, this is so cool. My invite code right now. Essentially, the phonetic spelling of what Joe Biden said when he was trying to sum up America in one word. Heaven, never put the foot in. What was that? Joking and cut that wasn't political. I don't think I just I just mentioned the president. What is it again? Hold on. Sorry, Jay Z. Like the rapper FT, like NFTs without the NB, like Booba. That was a callback joke that apparently only I found funny. It was funny. Ha, ha, ha. Thank you, Miss Glick. I'm cool cadet. If you didn't notice, did you spend all that time naming yourself cool cadet? I did. I named myself cool cadet. Good. That was definitely worth it. Wait, what is happening? What? What? Who are all these? I think we're a team against a team. Four of you four. So we're supposed to collect the crystals, right? In this mode, is that what this is? Oh, you're asking me? I have no idea. Well, the crystals are what give you the power ups. Well, the screen said collect crystals. That's all I know. On your guys' platforms, are you also getting about 60 FPS? It looks pretty 60-ish. We're on the same team here, bud. Oh, that's right. Are we not all on switch? See, I was confused because this game has cross-player on all platforms. Oh! You seem to be picking up what I'm putting down. It looks pretty good for being a switch game. I'll tell you that much. I think I'm really excited that I can play this game on my Xbox while you play it on your Nintendo Switch. Me too, Scoody. Also, I love the iconic words. This looks pretty good for a switch game. That's really the best we can hope for at this point. So I'm a pizza. What do I do with this information? Mm. Are you a Hawaiian pizza? Because if not, I don't want you. Wait, really? I will only eat a Hawaiian pizza. Aloha.   We won. We won. We won. We crushed it. Yes, we won. Yes. Top left, there was a little thing. Are you boys? Boys with an eye. For inclusivity. Ready to engage me in some fisty cuffs? Absolutely. Why don't you join the party that we're all already in? I'm ready to tussle. FPJZFTB would. I'm a minotaur. I'm a fast, fast boy. OK, this dude's riding a stingray. Sorry, what? Riding a stingray. I find that offensive to my culture. Wood, get in our game. What do you mean, get in your game? Get in our game. You're the only one not here. Wait, what? You guys aren't playing right now? No. No, what? Who am I playing with? I don't know. We've been sitting in the lobby. We've been sitting in the lobby, dude. Oh my god. I think you've been playing with yourself the whole time. That's not Lego friendly. That's not Lego friendly. All right, guys, we won. We won, everyone. Yeah. You did great tussle in that round, King. All right, let's reset. Miss Click, how you doing? Great. All right, we're controlling the point.  OK, I have a missile fist. We are absolutely crushing this point right now, and I'm so proud of us.  I think this mode is my favorite. This is pretty cool, yeah. No, stop, stop.  All right, cool. We have control, we have control. I have the shark. Shark bait, hoo-ha-ha. Shark bait, hoo-ha-ha. Shark bait, hoo-ha-ha. I'm not going to lie, this game mode is pretty sick. I love this. I know, I like this game mode actually quick. Only play this. I've got a fence. I've got a fence. Oh yes, it worked. That's actually a pretty clever way of defending the point. Ooh, ooh, ooh, I've fallen off though.  I've fallen over and I can't get up. Oh no. Oh no. I'm a fallen and I came to get up. That is my impression of American advertising. I want to boost the difficulty. Are we not playing against like real people? The game's not out yet. Oh, that makes sense. I told you that three times now. Victory, let's go. Hey, we did great though. Let's go. No, we crushed it. I'm so proud of us. We absolutely crushed it. Top brawlers, the ridiculous fighter. Let's go. Cool cadets. Hey, Scoot.  My God, what is Scoot thinking about right now that he's not, he's so checked out. We've said his name three times. Scoot. Yeah. Scoot, you having a tough day over there? Oh, sorry, I was watching a quick clip. I was curious.  Oh, I am Goku apparently. God, I am just rapid spinning this thing. No, stop sleeping. I'm just wailing on like a mini-fig as it sleeps. Oh my God, I too am now riding Mr. T. I like Mr. T. Is any cool? He is cool. I'm not sure who I should attack or not. No one's friends here. I'm not positive on that anymore. It's every brick for themselves. I don't like this game mode. Because I'm winning? Yeah. Yeah. I want to win. That makes sense. That was pretty easy. That was pretty par for the course for this guy. I don't understand. I wanted to hang out with the Monkey King and I didn't go get to. How do you win that mode? You had to get the peaches. I was not getting any peaches that I knew of. When you killed an opponent, you got a peach. When you tussled with an opponent. When you tussled with them, yeah. Until they could no longer tussle, you get a peach. Oh, so it's like kill confirmed? Well, it's like tussle confirmed. Tussle confirmed. You successfully tussed another person. I now realize our minifigs are pretty similar. Miss click. We're siblings. Final round, big stakes, winner gets $100. Woo! We're on the same team. Oh. We all win. Split four ways. We all get $100. $400. But wait, 33. We all get $100. Thank you. $33 each. I don't think if you split $100 four ways, it's $33. All right. You know what? Fine. If we win $100 each. I'm sending you the boost. Yeah. Ham, you owe me $100, so wear a wash. That's damn. That's true. By the way, if you guys want to watch a really fun video I was in on Ham's channel. I'll leave it linked down below. We go. Control point. Oh, it's control point. Oh, yes. It's the point again. Oh, we're so good at this. I have a bell, and I don't know what it does. I hit the bell. By the way, I'll point out, because nobody else is the fact that you can keep track of our progress, our winning progress on the top left. I've been saying that. That's insane. All right. Well, I didn't pay attention to what you said. Who is this magic genie? It's you. There is a magic genie on the point now in the area of the point. There is a magic genie. It's the magic genie. All right. Well, this was. We won this. Yeah, we won. We won. We won. We won this. Send me your pay pals. Scooty and misclick. $100. I love that mode a lot. Yeah. That control the point game mode is really where it's at. All right, guys. That was fun. Did you guys have fun? I had a lot of fun. Yeah. I liked it. Oh my god. I'm so glad. I always have so much fun when you invite me to these. Thank you for thinking of me. So I love the ongoing thing with misclick and inviting misclick to all of these. If Scoot and even Ham, if you want to join this little tradition, I would love to have you both in. All I'm saying is I'm not inviting Ant Dude again, because he pre-practice and ruined the video. He didn't pre-practice. He did though. He did though. Here's the good news. If you want to invite me to games like this in the future, I'm really bad at them. Oh, I love that for me. I can actually win. Everyone check out Scoot on Twitch, because I'm always on his streams. Check out Ham, because we have a video together on his channel, but also on Twitch. And if you're not already watching misclick, but you've been watching this channel for a while, I just don't understand you. You can watch a podcast episode that she was in. That's true. I hope you had fun watching this video. And don't forget to check out the game when it releases on all consoles, September 2nd. There is a link down below. If you had fun watching it, you'll have fun playing it. Okay, thanks, bye. Okay, love you. Bye, bye.


When you get your neck adjusted by a chiropractor, you're really in good hands. Sometimes in movies, you'll see one of the characters injure someone by doing what looks like a really violent movement to the head, and then you might look at a chiropractic adjustment and say, wow, I don't want to do that. How likely is that to happen? I'd say, let's probably just about impossible. The amount of force that we need to do that would be extraordinary, and chiropractors use a very controlled amount of movement. It's mostly speed. It's not a lot of force. Most people, it's something they find to be an odd sensation at the worst, and many of them find it to be a very comforting and good sensation. For those of you who are hesitant to have that done or just don't want it done, there are other approaches that we can take to still get that joint to move that won't necessarily make the click or pop. Lots of tricks in our tool bag.