A new study shows that abnormal production of nitric oxide in the body leads to the progression of breast cancer in mice. This finding could open the door to new treatments for early-stage breast cancer that stabilize the production of this pivotal molecule. Nearly one-third of newly diagnosed breast cancers in the U.S. are early-stage lesions. Though technically precancerous, about 40% of these lesions could progress to invasive cancers. Researchers don't yet fully understand what drives these insidious formations, but various studies have reported a common link between cancer risk factors related to lifestyle, such as a high-fat diet, high alcohol consumption, and low physical activity and abnormal production of nitric oxide. Normally, physiological stress triggers the production of large amounts of nitric oxide. This activates tissue-specific functions of neurons, muscles, immune cells, blood vessels, and other specialized cells. Under unstressed conditions, however, many different types of cells produce the molecule at the basal level to maintain tissue equilibrium. In the current study, researchers demonstrated that during cancer progression of breast cells, nitric oxide levels in fact plummet due to the reduction of the functionality of the enzyme that produces nitric oxide. To confirm how that process works out in a live animal, the team artificially blocked the mammary glands of healthy developing mice from producing nitric oxide. This suppression led to multiple precancerous lesions because of an upswing in tumor-inducing proteins independently of causing mutations. In contrast, the researchers introduced sepiapturin into precancerous cells. Sepiapturin is a naturally occurring compound that facilitates the function of nitric oxide-producing enzymes. After treatment, nitric oxide levels normalized, the expression of tumor-inducing proteins decreased, and precancerous cells started acting like normal cells. This finding strongly suggests that sepiapturin could be a powerful treatment for the hundreds of thousands of women diagnosed with early-stage breast cancer each year. The researchers are currently testing whether sepiapturin could also be used for breast cancer prevention among people susceptible to developing this disease, though realizing that potential will require more work. Delivered wholesale, sepiapturin could affect other tissues and organs which might give cancer an advantage. To eliminate this possibility, the team is currently developing a nanoparticle system for delivering sepiapturin payloads specifically to precancerous lesions in the breast.


How to make a narcissist fall in love with you? I'm sure this is something that many of you would like to know how to do because You met them They lied to you they manipulated you They deceived you They future faked They sold you a dream They made you believe in this future with them as though they were on the same page As though they wanted what you wanted as though they were about that for real and Yeah, you liked it you ate it up you participated in it You wanted to be a part of it this dream that they sold you this future Because That's just how it is When someone comes into our life and they present this Dream to us This illusion of love If you're a normal healthy person, of course, you're gonna want to be a part of it That's what you were looking for the only problem is we later realize That yes, in fact, we have been sold a dream It was a shared fantasy an illusion a simulation of love And after a certain amount of time This shared fantasy became something that we were just participating in ourselves Because they were never really about that They were detached from it It was just a means to manipulate you To get what they wanted from you, which as we know is narcissistic supply they objectify us They see us as something temporary for recreational use So that they can get attention and admiration from us, but then that's all that it was It had nothing to do with love and in fact, I will say that You can't get a narcissist To fall in love with you They're not going to fall in love with anyone That is never Going to happen and you will know that that is never going to happen Because they didn't love you They presented a dream an illusion of love to you and It never amounted to anything If anything all you got was abuse at a loving caring person They're never going to abuse you They're never going to do that at all and that is how you know that you will never get love from them You can't get blood from a stone The truth about the narcissist and why they are the way that they are Why they treated you the way that they did It's because they never got their emotional needs met when they were children They experienced a lot of abuse or neglect They never received love from their parents They never had any empathy coming in So now they have no love or empathy to give to you or anyone else All they can do is manipulate you and give you this illusion As I've said before the best you will ever get from a narcissist is an illusion And that doesn't matter if it's an illusion with you or someone else Because as we know after they discard us They go on and they triangulate us with this new person They display this false image of love of a relationship When in actuality that image that illusion It's actually no different to what they portrayed to you in the beginning When they manipulated you so no The narcissist is never going to fall in love with you And they are never going to fall in love with anyone else That's just not possible for a narcissist I've heard some people say that it is to some extent But in all of my research and experience I completely disagree with that And the reason why I disagree with it We can go a lot deeper into this Narcissists as we know are shame-based people Who are doing everything they can to avoid reflecting on their shame Pay attention to how you feel when you are around them That will tell you exactly what you're dealing with Do you feel the lower vibrational emotions Such as pride, desire, anger, fear, guilt or shame If you feel any of these emotions while you are around the narcissist That should tell you all that you need to know And it should reveal to you that they are never going to love you themselves or anyone else Because to experience love you need to be at the higher vibrations of peace, love, joy, acceptance At the very least you need to be able to accept yourself and the other person But if you look at narcissists they can't accept anyone They have a problem with everyone that they encounter Everyone that they get involved with And you will have seen that when you were out with them in public You could have been at a restaurant wherever it was There was always conflict There was always a disagreement There was always a misunderstanding So how can someone like that ever experience love? And yet many of us were looking at them and wondering why they can't love us Why they can't give us the love that we crave and need Why they can't give us the type of relationship that we wanted With loyalty and commitment Understanding and cooperation When the simple fact is that they just can't give that to you They can't be vulnerable And instead what they will do is be very cold and distant Especially when you're onto them Especially when you start to realize what you're dealing with Because as soon as you start to realize that You're not receiving any love in the relationship And in fact all you're getting is lies and manipulation You're just getting all of these stories of Why they can't love you or give you the type of relationship That they led you to believe that you were going to be involved in As soon as you start to catch on to that That is when they begin to devalue you They start putting you down They start making you think that you're not deserving of love They target your self-esteem When this is all just a distraction From the fact that actually they can't love you They don't have any love to give to you or anyone else And they're actually very ashamed of that Which is why as soon as you start to figure it out As soon as you start to catch on what do they do They project that shame onto you And then yourself loathing You're looking at yourself and wondering what is wrong with you When you should be pointing the finger at them And asking them what is wrong with them Because they targeted you for a relationship When the crazy thing about that is They can't even have relationships A relationship is the way in which two people connect But they could never connect to you A narcissist's idea of a relationship Is whether vectim attaches to them But they do not attach to you Because they can't attach properly to anyone They have an attachment disorder Which means that they also can't detach from you And that is why they get obsessed They develop this obsessive attachment Where they can't start thinking about you They always want to know where you are What you're doing, who you're with They objectify you They see you as this possession that belongs to them But at the same time they can't even love you They can't even give you a normal relationship And instead all you get is lies and manipulation Deception and future faking And sometimes you don't even get that Sometimes you just get devaluation Insults and put downs Where they project their shame onto you Because as I said they are shame-based people Who are doing everything they can To avoid reflecting on their shame And that is why whenever you're around them That's all you're going to experience And I'm sure those of you who have been involved with a narcissist It's something that we have all experienced Constant shame Because yes, unlike them, we can admit that We experience constant self-loathing We just feel bad about ourselves No matter what we've done for them No matter how much work we put in We just constantly feel down We don't feel good about ourselves When really it's them who should be feeling that way After everything that they did to you But they project their shame Because they're trying to run away from it And that's why if you confront them enough If you're really on their case And you just have this conversation with them As I have had with many narcissists in my past And it's just like, why can't you just be normal? Why is there all of this envy and jealousy Why are you so competitive? Why are you so insecure? Why is everything a competition? But you're always trying to outdo me Why can't we just be vulnerable and love? When you get to that point Where you're questioning them When you just want an answer Of why they can't behave normally Like a normal person When you get to that point That is when they will ghost you And you may never hear from them again If you're lucky Or otherwise they may just Paint you in a bad light And start a smear campaign against you Again to project their shame Which they are desperately trying to run away from They disown these parts of themselves That they don't like and assign them to you Because they don't want to deal with that And that's why we take on Their characteristic traits We begin to self-loathe We feel bad about ourselves And then they run off feeling a lot better Feeling like they're better than you Feeling like they're superior They got one over on you And as though something is wrong with you You're bad So it's you who couldn't maintain the relationship with them When we all know what it's like We've all been through it It's like we've all dated the same Person Even though we're from completely different parts of the world It's always the same thing with narcissists You cannot make a narcissist fall in love with you They don't have any love to give You can't get blood from a stone They have no empathy Love or compassion inside of themselves So they don't have any of that to give to you If you want to experience real love You need to look in the mirror Look at how you've been with the narcissist Look at all of the love you've given to them You accepted them without judgment You tolerated them even when they were manipulating you And putting you down You still stayed with them You gave them chance after chance You still accepted them And if that's not love, I don't know what is You gave them all of your love and devotion And all they did was reflect back to you Your own virtues and ideals They gave you a reflection of yourself So you pretty much fell in love with yourself And then they took on that role And it made you think that you were falling into something with them Sometimes you just got to stop and look at what they have trained to you And then look at who you are And you will see that they just mirrored you They imitated you They reflected you back to you While making you believe that it was them And if you don't believe that Then just look at it now Where are they now? If it was them If they really loved you They cared about you Where are they now? And that is how you know it was never them It was you They mirrored you They reflected you back to you And I know In this world I may seem like there is no love Love doesn't exist anymore It's old fashioned Now it's just social media Text messaging It's just sex There's no intimacy No connection It may seem that way But just remember you Remember all of the love, devotion and acceptance that you gave to the narcissist You're not the only person in the world who is like that There has to be other loving caring and accepting people in this world Although I will say that yes it is rare For my research I believe that it is only empaths who can experience real love And only one to two percent of the world's population Are empaths According to many studies So yes it is rare Fortunately we do have these communities And it does seem to attract a lot of empaths People who do care about other people But also because we've been around narcissists and we haven't had any empathy coming in We may find ourselves becoming more narcissistic Where we don't have any empathy to give to other people And this can be a problem as well Which is why it's very important that if you do find an empath Don't let them slip away That empathy is very important For you to share your empathy with each other Because as we know when we're around narcissists we get drained We don't have any empathy coming in So then there's no empathy going out And instead we find ourselves becoming narcissistic To where we just shut other people out Instead of Sharing this value that we have Because that's why we're here That's why empaths exist To share the love and positivity That is what it's all about And that's why I get on here every day To share these messages That's why I do this for you Because I know if I can get on here And I can give my love My empathy, my devotion to you Then I know that if you have this empathy coming in You will then have more empathy to share with someone else Someone who may even seem narcissistic But maybe they just don't have any empathy coming in for a long time Because that is a possibility as well Although at the same time we do need to pay attention to the bread flags But as for narcissists No You can't make a narcissist fall in love with you Someone who is shame-based Someone who is constantly projecting their shame onto you Someone like that they have no love to give They're not going to have any love to give to you or anyone else So I know this title may be a bit misleading But I know there's a lot of people out there who are thinking about this They want to know how they can get this cold hearted person to love them So I thought it was very important for me to make this video with this title So that you can discover the truth So that you can discover what is actually going on And I hope this video is helpful to you If it is you can show your support by giving it a thumbs up down below Let me know your thoughts as well in the comments Let me know if you agree with this message or not And you can share the video and subscribe And other than that I will talk to you in another video very soon You all have a good day


So good afternoon, my name is Anu Kanandare and I'm from the National Institute of Science Education and Research. It's in the eastern coast of India in a place called Bhubaneshwar and it had been set up as an institute of national importance to encourage research in the basic sciences. So I've been working here this summer in Professor Tom Martin's lab in biochemistry and the title for my project was effective mutations in the monk-13 homology domain of calcium-dependent activated protein per secretion, also known as CAHPS. So the specific interest in this protein that I was studying originated from a research in radiative thesis diagnosis. So exocytosis is a process by which cells package their secretory contents into vesicles and release them into the extracellular medium. While most cells have a pathway which is called the constitutive exocytosis, cells which are specialized for secretions such as neural cells or endocrine cells have an additional pathway which is called the regulated exocytosis pathway. It has three steps. First is called docking. First is called docking whereby a vesicle is brought close to the plasma membrane. The second is called priming which makes a docked vesicle ready for release. And this involves tethering the vesicle to the plasma membrane by formation of complexes between vesicular snare proteins and the target membrane snare proteins. Then there is a rise in intracellular calcium concentration. The membranes for the vesicle and the plasma membrane merge with each other and lead to the release of the contents of the vesicle. So the third step is done diffusion and the protein of interest, CAHPS, possibly acts as a step of priming and is known to increase the efficiency of vesicle release and it is also an essential protein required for regulated exocytosis but the exact way in which it functions is not completely understood. So I'll take you through a brief history of CAHPS and the questions of the ideas that led to my project. So beginning with the identification of CAHPS, so investigators of regulated exocytosis wanted to be able to reconstitute the process of calcium dependent exocytosis in an in vitro system and to completely understand what are the factors involved in this process. So what was done initially is that cells were taken, secretory cells were taken. They were cracked open and their cytosol was removed and cytosols from cells lacking a regulated secretory pathway or cytosol from bovine brain extract and other cells which have a regulated secretory pathway were supplemented to these cells. Following an addition of calcium, it was found that for those cells which received cytosols from cells lacking a secretory pathway, calcium did not increase, was not the process of regulated exocytosis could not be reconstituted effectively. So when bovine brain extract was added to these cracked cells and calcium was added to them after that, this effectively, the process of regulated exocytosis could be reconstituted in these cells. So this led to an exhaustive search for all protein factors in bovine brain extract that were responsible for these effects. So I had narrowed down to a 155 kilodalton protein which was later termed CAHPS. And it was this which was, it was seen which was responsible for this effect. So since CAHPS is a protein in verdant secretion, it is pertinent to ask whether the activity of CAHPS depends on the activity of snare proteins and investigators wanted to identify that in an in vitro system. And for this reason a lipid fusion assay was used. So two sets of liposomes were made. First one which contained a quench set of fluorescently labeled phospholipids, NBDPE and RHPE. And they also had inserted into their memory the vesicular snare protein bag too. So when these two lipids, the NBDPE and RHPE are placed very close to each other, the fluorescence of NBDPE can be quenched by a phenomenon called FRET or fluorescence resonance energy transfer which is explicitly sensitive to the distance between these two different kinds of lipids. So a second set of liposomes was also made of the same dimensions but lacking fluorescently labeled lipids and into its membrane were inserted the target membrane snare syntax in SNAP-25. So when there's a fusion between these two liposomes, these two sets of liposomes mediated by these snare proteins, the content of lipids from both these kinds of liposomes makes and there's twofold dilution of the fluorescent lipids that were originally present in just one liposome. So because of this dilution, the average distance between the lipids increased and FRET no longer happens because it exceeds the critical distance for FRET. Because of that, the fluorescence due to NBDPE is recovered and we can follow this up as an assay for that zone fusion. From some experiments it was identified that when CAHPS was added to this system there was an increased amount of lipid fusion which was seen and this indicated that CAHPS was accelerating liposome fusion in a snare dependent manner and also led to other additional questions suggest does CAHPS directly interact with snare proteins even though its function is dependent on snare proteins. So the next set of studies were conducted to address this question and they also used an in vitro technique using liposomes. So liposomes with snare proteins inserted to their membranes were taken and CAHPS was incubated along with these liposomes and this was subjected to density gradient centrifugation. So what we observe is that what we expect to see is that if CAHPS directly interacts with snare proteins which are inserted to liposomes it would be found in the liposome bump fractions whereas if it does not interact it would be found in the unbump fractions unbump protein fraction following density gradient centrifugation. Now what we see is that indeed CAHPS is found along with liposomes which indicates that it might interact directly with liposomes and secondly it again in turn raises another question is which region of CAHPS interacts with snare proteins? To address this truncation of the full leg CAHPS protein was created and all of these were again subjected to the same kind of experiment as I have just shown in the previous slide which is they were made to mount to snare proteins inserted into liposomes and it was seen that while most of these CAHPS fragments were incapable of binding to snare proteins there was a segment which indeed had the same binding property than the full leg CAHPS and it's localized to the LHD domain or the monk protein homology domain in the CAHPS protein but given that the length of this was substantially big it's a 69 residue long segment we still don't know the exact identity of residues which are involved in binding snare proteins so this is where my project found its context so the first part of my project was to mutate two consecutive segments within the 69 residue long fragment to other means and then observe the function of these mutant constructs in a secretion assay coming to look at it we find that a sequence that I'm going to be is there are some residues within these five segment regions which are conserved and whose functions are not known so they demand study and the second part of my project was to mutate another conserved five residue segment in the C terminal region of CAHPS which just preceded a dense core medical localization sequence the reason why this was of interest is because again the segment which has been boxed in red is conserved between several species and again we don't know what precise function it performs the experiment design that we adopted to answer these questions involved first constructing mutants of the CAHPS protein and flowing them into a retrosin protein vector TAC RFP by standard side directed mutated essence protocols followed by transfecting these constructs into HEC 293FT cells and then we observed the protein expression mutated retrosin's microscopy so subject to the condition that these proteins wouldn't aggregate when seen in the microscope we would then express we would then clone them again into a construct that would append a C terminal hexase gene tag to it and transfect them into hexase again following that we would purify proteins using standard nickel NTA column chromatography techniques and then test the activity of these in a permeable cell norepinephrine secretion assay a slight detour from the central focus of my experiments we also looked at the expression patterns of some CAHPS MHT1 domain mutants that had registered in the lab had previously made and the reason we were interested or rather he was interested in this part of CAHPS is because it exhibited some similarity to a snare motif and again these were very consumer residues and we didn't exactly know which functions it performed so upon transfection of these mutant constructs to HEC cells we found out that most of them would concentrate into NTA structures in cells so we were not exactly sure whether they were aggregates but given that the protein wasn't exactly soluble we did not go ahead with the NTA assay because effects due to misphoneded protein it could be a potentially misphoneded protein could not be ruled out when we observed all the other results so these are the other six other three mutants that we also tested and these mutants were also made by mutating these residues to alanines and the same kind of pattern was observed for all of them Coming to the mutants that I had talked about making previously so these were the expression patterns of the CAHPS 952-956 and 957-961 mutants both of these are within the macromology domain and it turns out that the CAHPS 952-956 alanine mutant aggregated again so since it was not soluble we did not use it for the RDE analysis but the 957-61 alanine mutant and the C-terminal mutant the RIDEG they seemed to express soluble proteins so we went ahead and analyzed them


Everybody forced here ripping one box of 2022 moment chrome baseball hobby here for Greg B. Appreciate the order Greg and good luck Yeah, Japan was really good. I thought they should I they had more chances throughout the entire game especially the first half So I thought They were better for most of it. They just kind of ran out of steam and once you get to pks The cool thing about pks is very dramatic, but it's also like I don't know It kind of takes away from the game a bit. Yeah, Japan keep improving a lot of nations can prove in though specifically Asian and African nations All right, good luck Greg some Marcelo Meyer shades of greatness insert green refractor rookie Juan Yepez 15 out of 99 Yeah First moment base auto say don't a raffaella In case y'all are just seeing that there is framed memorabilia in almanac I haven't even talked about Get real around here boys. Nice raffaella. You can make that Yeah, guys Friday. We'll have almanac personals $500 a box with memorabilia in it this time. It's the first time they've been purse. There's personals with memorabilia I'm a sailor be in a Invicta. Yeah for sure for sure Ash Yeah, they block of it was great classes Bulk is our base Let's see another nice auto to go with that raffaella Those two over there some of the bigger heads that'll be in it Oh Wow a refractor of Harry Ford number Orange shimmer First bowman blue J's auto Sebastian Espino 19 of 25 very good box here got a hot start tonight Nice Espino nice raffaella Edbert Perez inserts Shimmer yeti capping cruise base and that'll do it appreciate it Greg. We'll get these out to you


Kuuuqtอm zi. And what is it evolving to? This is the conversation that we are going have today. I am joined by one amazing podcaster by the name Kallab Chalo who runs the Blind Eye podcast. Karim? Ka leb? Glad to have you with us. You are here. Tuanziwa api lemi? Lemi have you introduce yourself the best way you are? I will introduce you but yes. The head. First of all I am a child of god. That is how I introduce myself now is... But I am a full-time podcaster and I work in a church. You work in a church? Yes, as one of the podcast managers. You run the podcast for my church and your church. Oh, that's cool. And my podcast too. Do people hire you to run theirs or set up a podcast? It's interesting. The kind of set up that I have is we normally go to record... One of my friends, his name is Yusembe, has a full set of podcast microphones and everything. And cameras and everything. So yeah, that's why I go record my podcast. And the church has its own set up similar like this. Okay. So when you talk about set up, we're talking about the physical set up. The physical set up. Microphones or the seats. You've been comfortable and everything. Yeah. Okay. All right. So where do we start? Let's start with your podcast. Before we talk about podcasts in general. Yeah. When did you start your podcast and why did you say the need to have a podcast? So mine I started around 2020 during COVID. Oh, during COVID? Yeah. I really had a passion about talking about a lot of things. One of my friends used to encourage me like, I know you talk about a lot this and this. Why can't you start a podcast about it? So I asked him what is a podcast because I didn't know what a podcast is back then. Like I didn't know at all. He introduced me to it, show me the ropes. Then he decided to sponsor me and bought me two microphones which I started with. And it's been a journey since then. Okay. First of all, I think I need friends like that. I'm changing my cycle of friends. Yeah. That's really nice. We should help you out. Because if they see someone who sees something in you and then helps you tap into that potential that you have in you to explore it. I think that's really nice. So your podcast, tell us about it. What do you talk about? So my first season I talked about mental health issues. I realized during COVID a lot of mental health issues were rising because the people were at home doing nothing. And parents were at home. So children and deserve issues, they came up during that time. So I saw the need of talking about mental health issues and educating my... I was a teen back then and the younger youth about mental health issues. So yeah, that's what I did in my first season. It went to ten episodes. I did ten episodes. So you do your podcast in seasons. Yes, seasons. I think most people do it in seasons. So for that particular one, the first one you did in ten episodes? I did ten episodes. Just mental health issues and because I had friends who were in that place. Some of them are committed suicide. So I was like, eh, enough is enough. We need to have a conversation about this thing. Let's talk about that a little bit because that's also something that you were talking about with my co-host just earlier on on mental health and it's something that everyone's talking about now. So what is your take as a youth yourself and something that was close to you? So what were you talking about in that particular podcast with friends? We talked about gas lighting. We talked about how depression in high school, how teachers can make you feel bad about yourself especially when they tell you you won't do anything, you're not enough and everything. And then bullying and everything. So even the politics in Kenya how sometimes it affects our mental state and the kind of things that we engage ourselves in. So that's one of the things I used to talk about. And I had a friend who did psychology, his name is Nick. So he came on board with me. So he started talking about how to surpass and get help and he actually organized different people who help. Like these are money, counseling. They kind of place institutions that you can get help from. So it was really interesting to see people actually tuning in and listening and even people engaging in the conversation. I like that. I was actually coming to that. How was the response? How do you get feedback from a podcast by the way? How was the reception life? The feedback. When you use Spotify for podcasters there's normally a place where you can allow people to comment. So from the comments you get the response or Instagram. I used to post on Instagram. I have a page on Instagram. So I used to post and post questions on Instagram and people would respond. So it got more attraction through that. Like especially Instagram. I got a lot of feedback from Instagram mostly. And my WhatsApp too of course. Alright. I love it. So it's podcast. It's on Spotify yes. But you use multiple platforms to sell it and people consume it differently because if I'm more on Spotify then I can get it on Instagram. Then I have your number. So one or the other I still consume your content. I think that's the beauty of multimedia now. The digital media. There are so many different ways of you getting content. Tell us about podcasts. A podcaster's journey. How is it fast for someone to start a podcast? Someone who's interested in podcasting. What do they need to do? How do they need to go about it? Do you go live? Is it usually required there? Give us the details of it. So for example, my friend bought me microphones. So after him bought me the microphones. I didn't know like I didn't do my full research on podcasting. But later on I realized this is something that I need to do for research on. So after having the microphones and everything you definitely need the microphones. The microphones are the key. They had to be. Yeah, the key. And for the microphone, I can advise you to buy a lapel mic because some of my friends started lapel mics because the audio quality is supposed to be... Key. Because people are just listening. People are just listening. So if the sound is bad, then I'm not consuming your content. Definitely. Alright. What types of mics are best? Rod microphones. Rod microphones. What they're quite expensive. That's one. That's one microphone. One. So you need money. You need a lot of money and funding. But that's what I'm saying. If you can't afford, then start with a lapel microphone because a lapel microphone is easier to access nowadays. And if you have a good phone, then you're good to go. Because if you have a good phone. And then a room that is not noisy. Yeah, is eco-friendly. And then you're set. Another thing, if you have people who can help you out. Like if you have friends or in the media sector. They can help you to set up how you can go about it. Because most of my friends are in media. So they help me out. Now we have good networks. We're talking about networks. It helped me a lot. Like having friends even coming on board to help me out. Alright. So the journey, it's not easy. I can tell you for a fact. You won't get paid easily. Especially with Spotify. I think in Africa they are not much invested yet. But we have a lot of investors right now in Kenya. Baraza Media Lab. There is... Like the African podcasters who are making it happen for us right now. Especially Baraza Media Lab. So how are they helping you exactly? How are they helping podcasters? They have a huge space. A mass space of all types of equipments. Cameras. And all you need to is just to get a membership card. And then that's it. You can record for a whole year. And they do your editing for your video and your audio. Like the best right now. I know they are really big Baraza Media Lab. But the membership is around 12,000. Yeah 12,000. I think that's affordable. If you can't afford... And we are not advertising or sponsoring.  Which is quite nice. So that helps. And if you have friends like me. Of course. They can help you. So it's a good space to be around. Especially the moment I entered in the podcasting world. So following Adele and Mantalk. You see those kind of people help me throughout to navigate my own space. The kind of contact they are producing is not the kind of contact. But we are in the same space. So the more we engage together the more we help each other out to navigate this space together. By engaging you mean you are listening into them. Yeah listening to the audio. Or do you actually are in contact with them? One Adele. She is helping me out with some few things. But her kind of podcasting is storytelling. Which is so interesting. Mine is being my friend Toki. Inviting other friends to come on board and talk with us. Like having a chat. That's quite interesting. So you have learned. I am getting the aspect of also just learning from those that are already there. For a starter if you are just starting then you need to engage with others. The content of others and learn from them. That helps you. And getting a listenership now for podcasting. How do you go about it? For Spotify for podcasters. It helps you spread your content in different platforms. So there is Apple Music. There is Deezer. And all those listening platforms. So it helps you spread your content there. But if you want to grow your listenership you have to be consistent. That consistency is good. So a lot of people will post once. And then they see they have two listeners. Then they give up. Me is the first time I had two listeners. Myself and my friend. Two listeners. But it grew from two. Right now I am at 100k listeners. Look at that. So I was like I didn't see that coming for myself. I had to keep on posting. Keep on posting. And telling my friend to repost. When my friend reposts. So it gets more attraction towards it. So that's how you grow your listenership. By spreading your content out. Because if you keep it to yourself. Then how is it growing first of all. And how your marketing strategy. Right now I am looking at starting my website for the podcast. So that we start going live. There is another platform called Rambo. Which is a very good platform. Especially for young guys. If you want to go live stream and get paid. At the same time. Spotify doesn't give the opportunity to go live. You can actually go live when you are recording. So there is no space for editing. If you are a person who wants to edit your content. There is no space for editing. So everything goes. Whatever you say goes live. Clear. So it depends on your preference. Exactly. So you can pre-record then edit it. Then post on Spotify. Because it has a space for posting directly. Okay. So now you are looking to expand yourself. You are looking to get your website. And then go to the website. And then start going live. To get more traction at the same time. Because when you go live you get paid for. Is it the numbers? Yeah. That's my goal now. That's what I'm heading towards too. All right. So now currently. Does it pay Spotify with your 100k viewers? Do you get paid for it? Certainly not yet. In him can you think Spotify is not quite invested yet. In Africa. Yeah, in Africa. But that's what I'm saying. Other people who are sharing as much as to make it possible for Africans to get paid for their content. But how am I getting paid? My job and myself. You job and yourself? I'm making sure that I invest as much as I can in myself to make content out instead of waiting for other people to pay me for it. So yeah. I have to be with myself first. So you have a job that you do apart from podcasting. Okay, investing in yourself. I love that. Apart from Spotify what other streaming platforms would you recommend for podcasters? Podcasters. I don't know. I forgot the name but it's one platform. But it's upcoming. Send your content there. What is it name? I forgot the name of this platform. I think Sema Box. Sema Box they're coming up with their own platform. It's coming up. I'm not sure when but they're opening an app for Africans to stream live their content and get paid for it. That's what I'm saying. There are people who are actually investing in this. So it's a space that's actually growing and there's potential in it for payment and making money in the creative economy. Because this is a creative economy. So there's potential. A lot of potential in it, right? So now I'm talking about diversifying your content. So we've seen podcasts that are live on other digital platforms like it would be it's just the mics you and your YouTube. So how do we differentiate podcasting from vlogging again? How would you define differentiating? I don't know. With this current generation it's just the people who want to see video people who want to listen to just audio so you have to go with the kind of trend that is going through. For example right now in Kenya people live the live visual like they love seeing rather than listening. So most people YouTube when I'm doing my own setup I'll have to have cameras around so that people actually see me as I talk and at the same time the same content that I've created I put it on audio but for me I'm kind of old fashioned nowadays I prefer audio rather than visual because with audio there's more storyline like the kind of authentic it is like people listening it's just amazing it's different, it's a very different experience listening to music when I'm cooking or when I'm doing my own things in the car there's traffic instead of watching I just listen you can just have your earphones on and listen exactly rather than having something to watch but the kind of generation that we're in right now people want to see Who is this voice behind this voice but to me I'm trying I didn't post a lot of videos on YouTube I don't know why I think you're sure I have a lot of videos pending that you have to post but all of the contents it's in Spotify already I don't know when you get it when you are ready for YouTube and the video there are some people it's the same for journalists with TV and radio some just prefer radio you're very authentic and I get that bit but now because I know with audio you have to be very descriptive for TV I can just move my hands and you can just see my face but for audio you need extra how do you give this extra the good thing I have a good co-host and my manager he is very quite loud his name is Jerry this guy is the director when you have a conversation there is that kind of freedom that we have we as friends we can talk about anything so the moment we start talking we haven't seen each other we see each other on a daily but at the same time when we meet and sit down and have a conversation about things there is something different that happens it's just magical I can see the word just magical because the kind of conversations people are tapping into right now it's different and nice at the same time I will tell young guys if you want to invest or want to join the audio space please join the audio space rather than visual because audio everything that's for you to decide which friend do you prefer my co-host would agree with you because he loves radio a lot but for some reason I prefer visuals myself but I wouldn't mind both so anyway now to content creation coming up with something that you want to talk about because you also need to know how do you come up with content that will bring traction to your podcast that will impact or what's your aim when you come up with content is it impact, is it entertainment what exactly do you aim at I aim mostly in impact because impact is everything one day I can share this one day I was just randomly walking and then somebody approached me and told me I've been listening to your podcast actually it's helping me out and she told me I get you, I understand you I was like ooh so that's the kind of thing that makes me want to continue producing the content I have friends who are actually good people on coming up with content they script everything from A to Z directly so this is what we are talking about and this is how we should go about it expressing myself as an individual I love that, tell me about it so you have a team I have a team, I don't work alone you don't work alone so you have a team and then they script so before you actually go and just people just don't go and talk so you decide before hand what you want to talk about you script by scripting I know it's not one to one but it's sort of an outline of what you want to talk about exactly so for example my second season which I did, I finished last year we were talking about finding God so my manager decided there is a conversation especially young people are trying to find something in their lives so we decided because personally I was struggling with my Christian work so I was like oh we should talk about actually finding God in different spaces that are going through and then we talk about adulting adulting is very hard right now complicated at the same time finding God in that space of growing as an individual so when he came up he was like oh this is actually really nice I didn't see this coming what so who comes up with the question my manager Jerry then you guys now we sit down we go through it first of all and see how we will go about it and then we sit down he gives us the freedom of even opening up and expressing ourselves expressing yourself the best way you can so that it's authentic so it's something you know that you can relate to so when you relate to it then people can also relate to it people relate to it and that's how you get people consuming your content the episodes the seasons that you've had since 20 20 that's a long time ago almost 4 years now 24 will be the 4th so what has been your best season a content that you created and then you tell us why I think I'm not yet in my best season but I can say my season 2 was like the oh moment the ah moment to follow my heart like talking about God because I'm not a lot of young guys talk about God right now people are in different religions and everything but my oh moment I'm saying my ah moment is because we went to that direction because we had a debate do we go in this direction or don't we go in this direction will people listen in or won't people listen in will people stop following us but you know I got a lot of criticism but at the same time oh so you guys actually listen actually listen so it makes me want to produce more of that content because they actually listen I'm not listening but they actually listen okay so even getting criticism means the people are listening in that's feedback okay very interesting and now back to consistency how do you maintain consistency or how before that how many types do you post in like a week or in a month so for us you normally record once every month we record like let's say for example 10 episodes at one day so it's a full day of recording we just block your day and then from that daily every week you post you post you post you post so you post weekly do you have a specific on Fridays you normally post on Fridays or Saturdays depends on if you have the audio edited earlier but you'll find by Friday on Saturdays you normally post okay so there's that you know someone will say oh we don't have time nini nini but just block a day block a day it's very flexible block a day that is flexible and talk about everything now you post it at your own time at your own time to convenience so that's okay that's good so what is it about consistency why should I be consistent why should I not post this week failed to post next week what's the power of consistency the power of consistency is that she will get more people listening and people will be loyal to you because automatically on Fridays I always listen to this type of podcast so on Fridays automatically when I post somebody who has been following me will know oh on Fridays there's something out for me to listen in so they will definitely go and listen in so the more people listen in the more you get traction and you personally will grow as an individual because you want to produce more content for them to listen in and it makes you accountable you know that for your listeners it's like you have been employed I have to deliver people are following me so expecting something from me okay that's something and how do you you've talked about growth so how do you ensure that you're growing yourself so that you just don't come and give people rumors okay you can give I don't post because it can be about anything but you can be a credible source of information how do you ensure you grow know exactly where your source is coming from don't just go there and just talking randomly because people will have different opinions but you have to go research about what you want to talk about research is key so that you can grow as a person because if you don't research about anything then it means you're lacking information so the kind of information that you're giving out is drawn so you have to do your research so that you can give the right information to people alright great so as we finish where do you see the podcasting space going or evolving to oh I see it becoming something big unexpected especially in Africa it's something unexpected I didn't see it becoming a big thing as it is right now but it's the next big thing so I'll tell every young person to invest on it because in the next 5 years trust me people making billions from that thing billions of cash from it so this is the right place this is the right place to invest alright good good enough I think that's a good place to close it unless you want to add something you're good for your podcast especially Spotify, Apple Music Deezer and Instagram of course definitely at the blind eye podcast the blind eye please spell the blind eye because I know it's not the it's the blind eye what it does and underscore in between the eye and the L the N in between the blind like there will have underscore eye people spell it the blind eye but it's the blind eye however you want to as long as you get there ok across all social media platforms all social medias even on twitter on twitter and x xnow this is called x we have moved on ok thank you very much Caleb for coming on board and sharing this amazing insights we will definitely follow your podcast and listen to your content thanks so much alright that has been Caleb Chalo he is the one who runs the blind eye podcast follow him across all platforms, Spotify get him on Instagram and wherever I'm sure when you get to Instagram or Spotify you'll know where the other where the other platforms are so make sure you follow him for that now we get to take a short break and then brand circle will be coming up next with another interesting conversation so stick with us