Welcome to MOOC course on Interactomics, Basics and Applications. This course will first give you the glimpse of the field of proteomics, different milestones which have led to the field of proteomics and where intractomic field fits into the field of proteomics. Do you want to learn high throughput applications from technologies like microarrays, next generation sequencing, mass spectrometry and label-free biosensors? If your answer is yes, so this is the right course for you. This course is a modified version of a workshop which we conducted at IIT Bombay. Distinguished foreign scientist Dr. Joshua Labair and several academic and industry speakers gave lectures and hands-on sessions. The other approach and the one that I'll talk about today is what I call a function-based approach. And the goal here is to look at the individual proteins and ask what do they do? What's their role? How do they behave? Who do they interact with? You know, are they altered in disease? And obviously these two approaches are complementary, right? They support each other. Through this course we aim to provide an interface between distinguished scientists involved in advanced intractomics research, industrial partners, faculties and students. This course would feature an intensive lecture series followed by some demonstrations designed to provide the much needed training required to explore the endless possibilities in intractomics research using genomics and proteomics that can be useful for researchers and students at any stage. This course will have weekly assignments and live interactive sessions. It has been really great experience in interacting with MOOC participants directly and provide them some live demonstrations of latest technologies from our laboratory at IIT Bombay. I'm sure you're now excited to learn these rapidly evolving technology platforms which have immense application in life sciences and translational biology. So I'll encourage you to join this course and learn many new technologies which are currently being used in the field of intractomics, genomics and proteomics. Learn the basic principles of field of intractomics and how you can also use it for many applications which are applicable in different domain of life sciences. I look forward to make it very interactive course and wish you all the best for taking this course and joining this for the next 12 weeks with us. Thank you.


Good evening everyone and welcome. This is Melissa Armo with the Stock Swoosh and I am looking here at Amazon. I did a video about 40 minutes ago where I predicted what Amazon would do in the earnings. It is doing as predicted and actually said it would gap up over 500. I said the target was 550 Atlanta. That's 550. Oh my, I just wanted to do this follow-up review because 40 minutes ago I just said what it would do and it's doing it right now. In fact it just went to the target. And actually this morning in the room I said what it would do as well. Oh my Atlanta. And Melissa Armo. Melissa Armo makes you money. If you did the call I made on Amazon. I told Surfdog about the option if you wanted to do an option on Amazon and is doing it right now. This is actually not even my gap rating system. This is just me, Melissa Armo. That sees things in live time that they're gonna do or sees things ahead of time before they do them to predict the move that the stock will make. And Amazon absolutely did it. So my class teaches, look at this. Oh my gosh. My class teaches how to train gaps after the gap happens. But I have something special, something above and beyond that which is me, Melissa Armo. Where I can predict how something's gonna gap and I do it. I can't do that in every single thing but I do see it sometimes. And actually this morning I said no chance of failure in Amazon. No chance of failure. That Amazon would not gap up tonight. I said no chance of failure. No chance of failure. Amazon gaps up tonight and gaps up over $500. Could be up to $550. I said really the target's $550 depends where it gaps. Wow. This is above $550 now. This is going to the dream target right now, live in the earnings at $415. It's amazing. So again, this is just proof positive of everything about the way that I know how to read money. I mean one of the reasons that I've developed my system that's successful to trade gaps after it gaps is because I'm doing really well with reading money. And that's how I can predict NCOF calls like this. I mean that's how I can predict when a gap's gonna happen or even when I see a gap and I say NCOF. I mean it's just really the fact that I really know how to read money well. So we'll see if anybody ended up doing an option in this last night or what happened tonight, tomorrow in the room. I just wanted to do a follow-up. Again Amazon's doing exactly what I said it would do. Amazing call by me, Melissa Armo and Stockswash. So have a great night everyone. See you in the trading room tomorrow. If you'd like to take the Golden Gap class, email me at melissaatthestockswish.com. Have a great night.


The force problems that we've looked at so far have had the forces either parallel or perpendicular to each other. But now that we've learned a little bit about vectors, we can apply these new skills to look at problems where we have forces in any direction. We're going to look at a book sitting on a table. Now if we raise one side of the table, at some point the book will start to slide down the table. Let's consider the forces and figure out what that angle is that we need to raise the table to in order for the book to start sliding. The first step in a problem with forces is always to draw a free body diagram. In this case we have gravity acting downwards, the normal force acting perpendicular to the table, and the frictional force acting upwards along the slope of the table. The second step is to decompose the forces into an orthogonal coordinate system. To do this we need to choose a coordinate system. Here there are two obvious options. One is to have the x-axis parallel to the floor and the y-axis pointing vertically upwards. The other option would be to have the x-axis parallel to the surface of the table and the y-axis perpendicular to the surface of the table. It's up to you to choose whichever coordinate system will make your life easier. In this case we have two forces that are already in the slope-table coordinate system. We have friction parallel to the surface of the table, and we have the normal force perpendicular to the surface of the table. So for this problem we're going to choose the coordinate system that's in the reference frame of the table, because that means we'll only have to decompose one of the forces, not two. So now we need to decompose the gravitational force into components that are perpendicular and parallel to the table. Let's call these components A for the perpendicular component, and B for the parallel component. It's important to get the angles in here right. These two are right angles, and so this angle in here must be theta. We can now do some trigonometry and work out what A and B are. So sine theta is equal to the opposite over the hypotenuse, so this is B on MG, and cos theta is equal to the adjacent on hypotenuse, so this is A on MG. So now that we've decomposed the gravitational force into its parallel and perpendicular components, we can redraw our free body diagram with all of the forces now in our new coordinate system. So now we're ready to apply Newton's second law. We need to do this twice, one for the forces in the x-direction and one for the forces in the y-direction. In the y-direction, we have MG cos theta downwards and the normal force upwards. Now because the book isn't falling through the table or flying off the table, the sum of the forces in the y-direction is zero, and we can solve for the normal force. In the x-direction, let's first write down a bit more information about the frictional force. Now because our book isn't moving, it's static friction, not kinetic friction, that we need to think about here. The static frictional force is always less than or equal to the coefficient of static friction, mu s, times the normal force. And so here we're interested in the point where the book will start to slide. So this will happen when we tip the table enough that the component of the gravitational force that's in the direction of the table is large enough to overcome the maximum static frictional force. And so the point we're interested in is where the frictional force is maximum. So we're going to set the frictional force Ff equal to mu sn, its maximum value. So now we can solve Newton's second law for the x-direction. The sum of the forces is equal to ma. So here for the sum of the forces we have the frictional force acting upwards, and mg sin theta acting downwards in the x-direction. So just at the tipping point before the book starts to slide, the acceleration will be zero. And then we want to solve for the angle theta. Now depending what materials the book and the table are made from, we might have a static friction coefficient of around 0.40, which would give us a tipping angle of about 22 degrees.