So I've been trying to think of a good way to talk about what trauma is and when I'm talking about trauma you know people immediately think of horrific events in maybe a nearly childhood and the truth is that because as children We're so incredibly fragile, you know, I think even physically we're very very fragile emotionally we're very fragile and Maybe we're around people and those people have had years to come to terms with their own coping mechanisms and neurotic behavior and Then you know the child is put into that environment and the child kind of has to come to terms with the neurotic behavior Did this functional behavior? It's not known to the child and it's not natural. So the child kind of has to adapt to that but These different experiences, you know, there's there's many different Adverse childhood experiences they range from neglect and abuse All the way to sort of a maybe parents as an example will be parents who will be overly involved which can lead to a different type of trauma and You know that word again trauma. It's not this it's just not just only these adverse childhood experiences It's anything and this is my definition of trauma Anything that leads to a split in the personality Or anything that leads to a split in the psyche so Let's take an example. Let's say for instance a Child is in an environment in which their parents are both Emotionally not attuned to their needs at all Or maybe even they're cold emotionally and what that child is going to learn in that situation pretty quickly is that my need my need for connection here is not going to be met and It's very difficult to not have an emotional need met for a long time So basically they will become emotionally withdrawn themselves They won't see any sense in reaching out for connection because they've tried and tried and tried and it hasn't been met however the truth for that child is that they do Truly want connection and that is not something that can ever change just because that need hasn't been met So in order to go along living in this environment With this unmet emotional need that's never going away The mind fractures into two parts they hurt part the part is that's been ignored the part that isn't getting what it needs Now becomes a liability from that child's point of view so that part has to go over here and a an Alternative part that has to cope with that is now formed over here and that is the part that is Deeply self-reliant it is maybe maybe even it becomes manipulative which it has to to survive Maybe it becomes fiercely independent to the extent that any child can and that's Now two parts of the psyche whereas before there was a unified part So that's one example now We we're gonna know this is kind of an avoidant attachment style that will take shape later on in this person's life in relationships but as This person grows up What they're going to learn is you know, we were aware of two different parts of the mind here now Okay, one that is fiercely independent Doesn't trust relationships because it a good reason not to and the other part that is deeply hurt and Still needs that connection desperately wants it. Okay. Now. What do we know about these two parts? Let's simplify this a little bit. Let's make this more practical these two parts their counterparts They Intensely dislike each other if you like any time we experience a trauma and there's this experience of a frack a fraction Fractionation or a Split The result will be two parts that do not like each other. They're kind of in a constant state of war or conflict with each other now There's really only One way to heal this okay, and the process itself is called reconciliation But we're gonna come on to that in a bit But the fact that these two parts hate each other and they're in such conflict with each other It really is down to the fact that that Person that child who maybe grows up now is Identified with one side over the other and typically what's the side that they're going to identify with they're going to pick the side of will say Are they going to pick the side of the part that wants connection and feels intensely vulnerable and hurt or are you going to pick the side of this Sort of savior or protector which is fiercely independent But you know doesn't give them that need for connection typically we identify With the protector part. Okay. Now. That's like almost a universal law and trauma the protector part It becomes the part we take the side of or we identify with Now in this example What we'll notice is in that period of the child's life Maybe in that difficult childhood they need to identify more with the savior part the protector part Well later on in life. They're going to run into relationships. They're going to still be and here's the thing even though To have identified with the protector this part. That's now and you could say it's in the shadow mind This part is still actively working to get its needs met it will still seek out Unconsciously to get that need for connection, but it's not consciously done. It's done unconsciously So the person will go towards Relationships, but the protector part won't allow them to stay there for very long as an example So Let's look at it. Maybe another example.  Well, okay, that part feels deeply hurt has an experience some conditional love here at all So it goes over here. It feels insecure doesn't know how to deal with it What's the solution? There's a split takes place from that trauma and now this other part forms which can become a kind of an intellectual or it can become You could say even and know it all it really feels like it has to know and master everything or have an opinion on everything and These two parts now intensely dislike each other this part here is Really completely ignoring this part that feels vulnerable at all and won't tolerate that vulnerable part It will never allow this part to say I I don't know for instance, okay We'll be identified with this Another great common example. I've talked about it many times here before but it's the example again maybe been shamed in childhood for being lazy and And very very difficult the child feels very very vulnerable and hurt because of that powerless even They develop a strategy and that's that split comes and the other side It is now the protector is going to be the productive guy. Okay, the productive person and Productive lazy Patriot between these two they don't like each other But you know, we're going to identify and want to be with that productive person and of course this lazy part is still unconsciously trying to get its own Agenda mere so it will find ways to act out that laziness unconscious unconsciously even though consciously this person is now identified as with the productive part so In that example, this person Will constantly be making decisions that constantly making plans They'll be trying to become more productive become more productive all the time all the while in denial about the fact of this insecurity or this very actual real valid part of themselves that wants really essentially rest and relaxation so Any decision they make while identified with one side only Will not be a good decision The whole process of healing in this no matter what the psychic explainer the trauma is that the vote that splits the psyche into two We only get into trouble with it and it's only maintained as long as we pick sides So I like the productive guy hate the lazy guy. I like to smart me.  See how we're picking sides What we have to do is take away or Disidentify with either side and start to see both of them as two parts of ourselves that are both quite valid and Once both parts have been heard what we're trying to do was not we're not trying to like give the The productive side something one day and then the next day goes to the lazy side We're not trying to get them to have an equal share We're not trying to get this one to compromise to accommodate this one or this one to compromise to accommodate this one We're trying to get them to both team up and We're trying to get them to cooperate. Do we're not getting them to? Sacrifice themselves right or compromise. This is not about compromise. This is about cooperation So practically speaking if we're trying to integrate this if we're trying to Disidentify from either of these two sides stop picking sides and see if we can actually just bring peace or reconciliation to these two parts of the One mind. This is all the one individual that has been splitted to do if we bring those two parts together We're going to have an experience much more of authenticity This is this is actually feels much more like me making decisions now Rather than me being in conflict and remember that conflict always comes from a trauma Or that split comes from a trauma. So on a practical level one thing we can do is What I ask people to do is try to try to identify what this conflict is And you know, you look out into your life and you look at your behavior You look externally and see where am I being incongruent here? Where am I telling myself consciously? I need to be more productive for instance and Then I find myself being totally not productive. I know it's unconscious without permission. It's taken over unconsciously So there's an example. Okay, there must be a conflict here in myself. I'm telling myself.  Look to it What kind of a name will be appropriate for that guy? And you know a person might say well, this is my inner slob I've used that term before this is my inner slob And I see that guy just sitting back on the couch and he's just eating pizza and watching TV And so there's the inner slob guy and then there's this who's this productive guy? Well, this productive guy is He's a machine. This this productive guy never stops. He's always on point and he's really really efficient So this is the machine versus the the lazy slob will say and Now what we're going to try and do is see see automatically we're going to identify with the machine Okay, now the problem is the more we identify with this one. We're actually strengthening this one That's another rule. One of the rules is they both hate each other. The other rule is The more I identify with one and building up the other one simultaneously both sides have to equate. It's like a Weighing scale that has to be perfectly balanced. You can't really be more with this one because They can't really be split that the shadow persona aspects of our psyche always have to equate, okay? So what if I have a big persona?  I'm not going to pick favorites And I'm going to ask them both what they would like to do for the day or the week And you know the machine guy is going to be like we need to do this we need to do that We take care of this you don't have time to be lazy It's going to want to do a million things and the lazy part then We're going to give it an equal share here. It's I'm tired. I don't want to do any of this stuff It and you may notice that they actually start fighting.  This guy is not going to help you I'm the only guy that's here to help you if you keep listening to him. He's going to drag us both down Are we seeing the conflict? Right, this is why we don't experience peace of mind. This is why our mind is so full of conflicted thoughts all the time so What we're trying to do is to and allow this sort of Dual conversation to go on within us and eventually get to the point Where they can both okay look if I refuse if I listen to both of these sides with openness without picking a favorite I'm going to sort of sit back and wait until they can both come together and reconcile And really that really comes through validation of both sides a Big mistake that we'll make here is is to throw the machine guy Under the bus that's a big mistake people make to okay the machine has been the protector for us in this So We validate the machine we validate that hyper productive drive that's there Yes, it's coming out of trauma. It's a result so that we don't feel shamed anymore, but it has protected us At least it's held out the promise of protecting this So we validate that and then we validate the other aspect the part that feels Overwhelmed or it feels like it can't go on or it just wants to rest and relax And the more you validate both of them and they both feel heard to be more willing than to cooperate And you'll come up with some sort of a more balanced Plan to move forward that would make a lot more sense. It's going to be a lot more Doable and there'll be no unconscious undermining of that plan because the lazy side of it has signed up on it So If you're experiencing if you're listening to me speak now and you have any resistance to what I've just said there Realize something you're probably identified strongly With the productive part of your psyche. Okay, the productive part has no time for laziness. It has zero tolerance for the lazy slob guy, okay relaxation and Unfortunately that is not going to work because unconsciously the the other aspect will undermine it So this whole process you could call it reconciliation. You could call it Just taking back allegiance to one side or the other and Over time what we'll find is our mind becomes less fragmented we're going to be less and less conflicted within ourselves and No matter what the original trauma was whether it be due to shaming about being lazy whether it be about Just being abandoned and not feeling safe in life. That's another great example right there. What happens when that happens? well One part of this is going to feel terrified and not safe And we can't go on with that in an environment in a dangerous environment So another part comes it becomes a protector and it may become very very hostile or aggressive externally So these two parts later in life then when we do get to realize and this is Unsustainable this aggressive part that I've identified with is taking over my life and it's beginning to get me into a lot of trouble, right? I'll secretly I'm feeling very insecure and afraid all the time We're going to have to reconcile those two parts We will be deeply identified with that protector. Okay, it becomes our identity. We become this sort of intimidating person So that's another example. There's many many different ways in which we We fracture ourselves Or we've been fractured to be honest because it's never the child's problem falls but this whole process of reconciliation is Something that requires it requires stillness and it requires an honest examination of How my behavior seems to be contradicting what I am consciously Stating I want in life So in that example of the person who's been aggressive then they even tell themselves regularly, okay at this point now I have to stop with this aggression. I have to really start now to Put that away But that aggressive part if that's been put into the shadow pattern out that will come out So that's because I'm now hating that part of myself and I've started to identify with the other part of me so This is the type of work that you'll find in inner child work a lot of this stuff comes out We're not even aware of it But I tell people if you want to know who you are if you want to know what your personality is Typically when you're starting off that journey, you're going to look inside yourself and you know, you're gonna find you're not going to find your personality you're going to find an Aspect of your personality one part of your personality that you're deeply identified with Okay, so the job is to identify. Well, what's the opposite of this part of myself and now we're getting somewhere What part of myself have I denied? That has led to me formulating and identifying with this One part of who I am of my personality So this is called shadow work Family systems therapy also has elements of this looks at the different ways that people fraction often and Take on roles given the circumstances that they find themselves under the adversity that they find themselves in and The thing about this type of work is that it is incredibly transformative and it is the quickest way to end inner conflict right it's very compassionate work and I have found that it's probably 10 times more powerful than than any sort of behavioral modification you can try to do or You know, even something like gratitude listing or anything on the positive side of things which I believe in and I think it has a time In a place this is much more transformative than that and it's a deeper level of work to do So really what you're looking for is where am I conflicted? Where am I at war with him? I said with myself internally and I mean the book I wrote on procrastination for instance really went off this entire model It was just entirely about that whole Conflict that comes around Identifying so heavily with the productive person. Okay. Well, what's over here that I've been ignoring. Oh shame Really deep shame and it's security and low self-esteem Maybe it came from trauma Maybe I need to reconcile these two parts of myself and watch what happens, you know It's very difficult to be in touch with your power the power of your own personality if you're split in half It's almost impossible So guys, I'm gonna leave it there for now But if you have any comments on that or you want to contact me, you can do do so below or through my website and Hope that was food for thought and I'll see you again soon. Take care


In this game, I will be asking a popular YouTuber the price of 10 Yu-Gi-Oh cards. Their goal will be to guess the price of each card as closely as possible. For each dollar away they are from the correct answer they receive a point. As points add up, they'll lower the price. Today we see if your favorite YouTuber can win the $1,000 grand prize. In today's episode of Guess That Price, our new guest is SEMO. SEMO is one of the largest Yu-Gi-Oh creators on YouTube, having amassed over 300,000 subscribers. He's well known for his extremely popular show, The Progression Series, as well as his much anticipated banless reactions. Today we find out if he knows his Yu-Gi-Oh prices. Welcome back to episode 25 of the Auction Series. Okay, okay, maybe not. Not Auction Series, but we do have another series and SEMO is here, guest number three on our new price question game. I don't even have a real title. So SEMO, how you doing? I'm doing fantastic, my friend. Now, when we're bringing back Auction Series, I know the audience really clamoring for it. I don't know. I'm hearing a lot of buzz. I'm hearing a lot of hype. I mean, it feels like Auction Series could be back at any day. I guess we'll have to find out in the future. I don't know. You've been gone for like six months. At least. Yeah. You've been traveling the world, meeting people and stuff like that. But now you're back. You're back to Yu-Gi-Oh. And you better know the prices or you're going to be in big trouble. So the fun part of me doing this, especially when you're telling me about this idea is that I've been out of like modern Yu-Gi-Oh for a while because all I know how to do is apparently make series where we start at LOB and make our way up. So as a result of that, I'm the perfect candidate for this because I'm probably not going to know like 90% of this. We're going to see how well my knowledge has stuck over the years. We're going to have to see if you remember some of those prices because we've had two guests so far. They've both done fairly well. We had like an 11 and a 16.9% away from like the correct prices. So pretty solid. But I think Yu-Gi-Oh guy, you have an advantage. But you did kind of take a break. So we're going to have to see, let's get right into question one. Are you ready? I am. Yu-Gi Boomer Edition. Let's go. This is a card that I know you're very familiar with. So we're going to see if you're familiar with the price. The first one is Break of the Magical Warrior, Magician's Force, Ultra Rare, First Edition, Nearmint.  So I do know with this that this isn't technically the most expensive highest rarity because the most expensive is the TP7 Super, which I disagree with by the way. Like I understand it's rarer technically, but the Ultra just looks cleaner in my opinion. I definitely agree. I think that this one should be worth more. But I will give you, you are correct. It is definitely not the most expensive version. The TP7 is. Do I get points for that? Do you get any points? No. No points for you. OK. Well, at least I know that much. This is hard because like I have had this forever and like obviously I didn't get it for what it's probably worth now. And so it's definitely gone up significantly over the years. I'm just going to, I'm just going to take an easy guess. I'm going to say that this now first ed, MFC, I'm going to say that this is a hundred bucks. So you think it's in a tier one, the very top of tier one is what you're feeling. Well, I guess it's by tiers, right? So yeah, so you got to keep in mind the tiers will help you like later on like what it is. So you are hitting the very tip top and max amount of tier one. I think to be safe, I'm going to go tier two because I think it's going to be like slight. It might be like 110, 120. So let me actually, let me change up here because I did forget the tiers are part of this. I'm not just like trying to guess like immediately on the head. I'm going to go $120 tier two final answer. The answer here is $90. No! So you are going to get the right tier and get a $10 credit and have it right on. But then you decided to bump it out to the second tier. I understand the logic though because it does seem like it would be more of a tier two card. It seems like I looked at the listings for this because I was a little surprised it was 90 bucks. Like most of them were over a hundred. Like three of them were in the 90s. It was like 90, 95 and 99.  Like you guessed. So you're pretty much right on the head. It's just the lowest one that someone had put it at 90. I think it was at 95 as well. So there's a couple people are selling early. But honestly, you're only 30 bucks off. You get to throw out one. So you're going to be fine so far. Let's go to number two. Riza the storm monarch forcing the breaker ultimate rare first edition near mint. Beautiful. I should go, I just need to go get in my collection and just bust all these out. Yeah. You can just ask the cards. Hey, how do you work? Okay. I think this is going to be like similar to like breaker where this I believe should be like tier two. I think it should be like within like that hundred, like low hundreds range, maybe like mid hundreds. So I'm going to go with tier two 150 on ulti Riza final answer. So far you feel like I feel like you have a good grasp of what the values are at least kind of close. So let's see how close you were on this one. You said tier two on this one. I'm going to go in and confirm you're correct. It's in the second tier. All right. We're going to get the bonus.  We're depending on where you're missing it from. Riza storm monarch forced the breaker ultimate rare first edition near mint this card. You said 150. I said 150. It is worth one hundred and sixty dollars. Whoa, almost right on the head. So you essentially got zero with the bonus. You almost nailed it. Okay. All right. Yeah. That's that that makes sense. I feel like that is what this card is. So far I'm getting a little bit worried that I'm going to have to give away a thousand dollars after this. So hopefully that does not happen. Okay. Maybe that would be cool actually. So let's see the next one. Question number three. Here's a card that I don't really know much about. I know that you might know about this card. Salamangray pyro phoenix from what is this chaos? What is chime chaos impact chaos impact. Okay. Starlight first edition. Oh, the second starlight set ever. So I know you and Salamangray. I don't know about pyro phoenix though. So yeah, we didn't really play this card because it's kind of bad. Okay. All right. Why didn't you get a starlight then? Because they make a lot of starlights bad. So this was back in the era where they had to use like a rare as a starlight each rare. Super. So dumb. Like what were they thinking? Let me let me take a step back. This card is like okay for what it's worth. But it's just like in the context of like when you played it and everything else at the time, it just wasn't great. Right. So this is the thing. It's a starlight, which you immediately hear starlight. You think this is going to be like a billion dollars, right? Right. Yeah. Essentially this is, this is a bad starlight. So this one's not worth a billion dollars. I should not take a loan after this card. This is not IP Masquerada. No. Yeah. The other one. The thing is though, it's still a starlight and the old like, so then you have a card like win, for example, right? Which win is like objectively the worst charmer. But because it was like one of the first starlights, it's like $800 or some like ridiculous price or something. Those are insane. Those charmer cards. But I don't know if the second wave, aside from like, I know IP is up there just cause IP is actually playable, but I don't know where Pyro Phoenix falls on the spectrum. If you don't have a good idea, you do have three lifelines. Keep that in mind. I do, but I feel like I'd rather save them for later down the line. I think I have like a general idea with this. I'm just going to, I'm going to flow to guess out there. Interesting. Okay. I think it's too rare for it to be tier one. The early starlights especially are also like worth money cause of like the fact they were the first ones, but there was also been bad starlights. Like there's also that, there's that trap card too, like the storm wind, whatever. The storm, what's it called? The dragon's storm force or something? Yeah. Something that like it's bad, right? But that even that card is worth money in starlight rare. I think I'm going to try to go a little bit safe here. I'm going to wager that this is tier two and I'm going to go for 150. I'm going to go like, I think this is worth about what the rise is worth. Okay. So you, you are guessing 150, the same guess as you did on question two. Yes. You're locking that in. That's your final answer. I'm locking that in. You don't want to go off, you don't want to change it at all. I feel like that's what this is worth, regardless of what it's actually worth. Okay. All right. All right. He submitted that he will pay $150 for a solid man grade pyro phoenix. All right. You heard it from the horse's mouth. All right. Number three, you guessed $150. The salamangra pyro phoenix from chaos impact starlight first edition near met is worth $83. Wow. So was tier one. So if you have one sell it to Simo, he's apparently a double for it. Yeah. I thought this would be a little tricky cause it's like high rarity, but it's worth essentially like nothing compared to most starlights. I figured it would just be like at least over a hundred. Like I know there's a few starlights that like are incredibly low, but I didn't think there would actually be below a hundred. So that just goes to show how bad this card actually is. I was going through the starlight list and I was like, man, this card is cheap. I was like, all right, let's just go for it. You didn't hit the tier correctly, but you do get to wipe away this question. So it's no big deal. It's not a big deal because you missed this one. This one's probably getting wiped away. Yeah. That was pretty hard. Yeah. Right now, it's really not that bad though. You're only off by $67. That's really not too bad in the grand scheme of things. If this is your worst one, you're going to do really well. So let's move on to number four. We have the needle worm from TP3. It's an ultra rare Nirmit condition. This was the one ultra rare out of turn of the pack three. What do you know about this card? Do you remember playing the old school, like eternal duelist soul and like worldwide edition and you'd play up against, I don't, I think maybe it was like Bakura who played the mill deck. I don't remember. And the whole deck was just playing up against needle worms and just grave keepers, servant and all that. That's basically my memory of needle worm in a nutshell. So you could get it for the cost of eternal duelist soul. Maybe a few copies of eternal duelist soul. The digital version. Yeah, the digital version. Yeah, that's true. So the little guy, TP3 anything tournament pack is going to be money. Like even the comments in that set are money. Yeah. This is going to be up there. I think TP3, this is probably a TE3, tier three at least. So that's at least 250. Yeah, TP3 needle worm has to be at least 250. Where it's going to fall in tier three is a different question. You do have those lifelines, keep in mind. You can do a lower or higher. That could help. You could even get a new question if you're not too sure about this one. I think I like the challenge here. Okay, all right. He's going to try and run the gauntlet with no help. I want to run the gauntlet. If the very least, I think I don't feel, I think I feel pretty confident. I'll be like somewhat close. I'll probably regret saying that now that I just did, but I'm going to say $500. 500, you want to lock it in at the 500. Final answer. It would be by far the most expensive card. So let's see. Okay, you guessed $500. The needle worm from tournament pack three, ultra rare near mint is worth $600. Not bad. Not bad. You're pretty close.  That's really not too bad. So technically this one will be getting wiped away now because it's more. It's further than the Pyro Phoenix. Percentage wise, it was better. It was a better guess. So this man's trying to do it without lifelines. I see. Okay, all right. I want to save them for as best I can, which I could have used it there maybe. Then I would have had a better idea.  So it's like, you kind of had a ballpark idea. So maybe not use it there. All right, let's go on to number five. Blackwing value, the emblem of honor, ancient prophecy, ultimate rare, first edition near mint. Initial thoughts. This is an Edison format card, 100%. And with Edison being as popular as it is, this is going to be pricey because everyone who plays these alt formats, they're either one of two ways. They either want to just have the cheapest version of the deck possible just to play like pickup games or they want to have like the max rarity Edison stuff. Right? That is completely right. If you're going to play value turbo, you need to have value, right? And having an ultimate first ancient prophecy value is basically mandatory at that point. Yeah. So like what level are you saying? Like what level do you think you think it's going to be all the way at tier three? You think tier two? I don't think it's going to be tier three. I think it's probably going to be in the tier two area. If this is over $250, I'm going to go quit. But I feel like this is it's money, but it's not like needleworm money. Okay. Just because there's it's a later set. There's more of them available. I am going to wager that ultimate first near mint value is $180. Oh, that's pretty expensive for a little blackwing guy. $180. Okay. So you want to lock that in at 180? Yes, 180. Question five, blackwing value, the emblem of honor. I like how the blackwings all are like blackwing and then they just like have another name. Yeah, it's like a dash. Like I noticed that when I was looking through the blackwings to figure out which one I was going to ask you. I was like, okay, these are got some long names. So via the emblem of honor from ancient prophecy, ultimate rare first edition near mint. This card has had the craziest rise. So it was easily like down in the tier one just several months ago. Yeah. It was like, I think 30 bucks or something like that. Or like 40, 50. It is now all the way up to $298. Holy. The progression series is over. Cimo has quit. Oh my God. I quit. It's too expensive. I've quit. It's too expensive. I knew it was going to be up there. Because again, Old Ulti's first ed especially and it's playable and it's in a form that's the most or second most popular alternative format that people play. But I was not expecting nearly $300. It is ridiculous how expensive this card is. I think maybe a factor is because ancient prophecy was one of those boxes that didn't have a first ed booster box.  I would assume that's part of it, but also it feels like it's got how to buy out like and like people just aren't reselling them. So I think the other part of it too is that it's also like one of, if not the best deck in Edison as well. I got waxed by it several times this week. As a result of that with again, more people getting into Edison, it's probably just spiking the demand even more for people who want to play if they want to just high rarity the whole deck. So I mean, I just didn't think it was that expensive. That's insane to me, 300. I mean, to be fair, at least I didn't say it was like $50. So yeah. You were definitely on the right track. You were talking out why it was good. So you just didn't have the exact number. All right, let's go to number six. So far, reminder, two tier three so far. We've got two tier ones and one tier two. Keep that in mind. We're going on to number six. Yada Garasu legend or not legend. Legacy of Darkness, secret rare first edition near mint. This is an interesting one cause this just got recently unbanned. So not on the most previous list, but it was the one before that I believe. Yeah, I think you're right. Yeah. This is funny because I remember as soon as this came off the list, everyone's like, Yu-Gi-Oh's over, let's quit. Like Yada's back and the only deck that could like maybe do anything with this card is like flunder. And that's a big if and then it didn't do anything.  The one thing that Yada has going for it, actually two things, let me take a step back. The two things that is going for it is that it's a very old, very nostalgic, very memorable card for a lot of people. And because of that, it's also just going to be expensive because it's a first-ed secret. The secrets especially from the old sets like LOD fifth set ever printed always seem to hold up very well. So I think this is a tier two. I feel like this could easily go, I think even before this got unbanned, it was probably like a hundred dollar card for the first eds near mince. I feel like that's kind of like a reasonable price for this card. Then if we're going to account for ban list type and then crash, I feel like there's probably a bunch of people who held them and just want to just maybe sell them at a higher price. So I'm going to go tier two.   It feels like a lot of cards around 150.  Evidently. Yes. All right. So you're locking in $150 is your guess. Yada Garasu from Legacy of Darkness, the secret rare first edition near mint copy is worth $160. Oh, so close again. How did I do that twice? I guess 150 on two 160 cards. You actually got it exactly right that time again. That seems about right. I feel like that is like accurately where this card should be valued. Question number seven. It's a card that you may have heard of. Blackwing collute the moon shadow legendary collection. Five of these first edition near mint. I have single-handedly probably cause the value of this card to spike tremendous. I should say single-handedly gauge is also a big part of that. Yeah. Yeah. You engage combined. I've probably made that happen. I don't know if you're allowed to clarify this. I believe this is the ultra rare. Yeah. I meant to put that. I think it's an ultra. I can't tell that picture. I'm pretty sure it's an, I think it's the highest rarity of collutes. It is the highest rarity. Yeah. I'll tell you. Yeah. Okay. That's fine. That's all I need to put the rarity and I forgot. Okay. So yeah. As I was saying, because of the progression series, because of the meme and collutes, basically my mascot at this point, collutes have just gone through the roof. Also, I will say Edison has also had a fair bit to do with it as well. Yeah. But I think Prague is pretty much the biggest park is people just want me to sign collutes and gauge by extension because that man's probably signed like a thousand collutes at this point. So this is a tier one. There is, I refuse to believe that. You don't think you can get over a hundred? I refuse to believe that ultra rare collutes are over a hundred. If that's the case, I'm actually quitting. That is, that would be unbelievable to me. This is tier one a hundred percent. Now we're in tier one is a very different story because I could see this being like maybe 50, 60, $70. Like I could see that actually. I don't know if it is a hundred. You can see 50 through 70, but you can't see a hundred. I refuse to value this at a hundred dollars. Okay. All right. So with that being said, how much do I value this with Edison, with all of the merit in progression? Like everything surrounding this park. How cool are you? That's what you have to really think about. Really? That's what it comes down to. Yeah. And I'm pretty lame. People care about Seymour. I'm pretty lame. Let's be honest. So I've met you now. I know you've done 20 something episodes of auction series with me for some reason. So I'm going to go. I felt confident for a second and then I took a step. Hey, you have three lifelines. I do. I'm not using them. You can do it over a hundred. This might be the perfect one. I refuse to use them. I refuse to use them. Blackwing collute the moon shadow, ultra rare legendary collection, 5D's first edition near mint, $35. $35. Okay. So about a third of a hundred. So you're really sure it's not over. I think a play set of this could be worth a hundred. I don't think a single copy is worth a hundred. Okay. Okay. All right. So you're locking it in 35 bucks for Blackwing collute the moon shadow. The answer to this one is 17 dollars. Oh, I was way off. Okay.  So it's really not that much to think about it. The thing is the collude also has a lot of printings, which helps it as well. Yeah. It did have like four or five, I think. I remember at one point, this was ages ago. I think it was maybe when Prague first aired with Blackwings and like I was on the whole Blackwing streak. I think the collutes were pushing 30 around that point. So I wasn't sure. That's totally right. I think in my head, maybe I was thinking they stuck around that, but it seems like they cooled down a bit. And honestly, I feel a lot better that this is a $17 card. Yeah. It'd be bad if this was over a hundred. Yeah. No shot. No shot. So far we've now hit three tier ones. Keep that in mind. Two tier twos and two tier threes. We have three questions to go. We also have three lifelines which you are refusing to use. We'll see if you can continue that streak or not question number eight, Dark Arm Dragon, Phantom Darkness, Secret Rare, first edition near mint. I'm going to make a lot of people angry with what I want to say. I think similar to the breaker thing, I think this version should be more expensive than the old tea. Over the old tea. Okay. I love old teas. Anyone who knows me knows. I love ultimate rares. I think it is a sin that the original first ed secrets are not more expensive than the old teas. Okay. And unless something changed recently, I'm fairly certain that that's still the case. But the old teas are like champion pack or turbo pack, something like that. Yeah. Turbo pack six, I believe. I get why it's more expensive and people like old teas too.  So today you've quit Yu-Gi-Oh! You've assaulted ultimate rare lovers. I feel like you did something else in the beginning too that was pretty debatable. Probably. My gut's saying tier three. I feel like if this was not first edition, this would probably be in like tier two. Because especially with the rise again of retro formats and such. And again, this is another heavily nostalgia based card. If only you had a way to know which tier it was in. If only. Well, this is question eight, isn't it? It is. It is. So I could use one of my lifelines here if I really wanted to. But I feel like you couldn't use them before. Ah, now I can finally use them. Question eight. To be fair, if I'm under the assumption this is a tier three, I think this is probably a good point to use it because had I used it on like the needle worm, I could have gotten a lot closer in that sense. So this is probably another good point to use it. You also could do, not only to know the same tier, you could just do lower or higher if you'd figure it out. That is true. That is true. I'm pretty confident that this is tier three. Man, do I even want to use a lifeline? It might even be better to save it. I feel like I'm pretty. I guess technically you can use multiple on the same question if you want to. I was about to ask that as my qualifier. But yeah, if I'm allowed to use multiple, cause I've been in the first episodes, everyone used them in the first like three questions. So this is new. This is new.  I'm like, actually I'm going to save it because I think, I think I feel pretty confident. Tier three, secret first near mint dark arm dragon, Phantom Darkness, beautiful card, $325. 325, you are feeling confident that it's a tier three card. Cimo, you could have used a lifeline. I'm just saying you could have. To know what the dark arm dragon, Phantom Darkness, secret rare first edition near mint, you could basically guarantee they were correct here and you could have potentially saved yourself. But you didn't need to, it was $398. So yeah, you're still pretty close. Yeah, you're still pretty close. Yeah, I was going to go 350, but I thought it could be like, I wanted to just hedge my bets. I feel like these went up cause I feel like they used to be like under 300 at one point. I feel like that as well. Yeah, I think these have gone up just again. People feel like you, maybe they want it to be better than the Ulti. So it's kind of in the same ballpark almost at this point. It's interesting cause I feel like a lot of the people who play the retro formats, unless they just collect it for the nostalgia factor and don't care about playing, I feel like retro format people also like having the original versions of the cards that were played at the time. Not like the newer versions, right? Yeah, also I feel like the actual Phantom Darkness is like more sturdy than the TurboPak version cause the Ultis are kind of like, you know what I mean? They're kind of flimsy. They're flimsy, they're flimsy. It's like if you're actually shuffling the card, you feel better about having the Secret Rare cause it's a little thicker. I don't, I just feel like the Secret Rare just, I don't know if it's the inking on the card or the coloring. It just looks really clean. And the Ulti, I don't know. I feel like the Ulti doesn't really do the card justice and maybe this is just, you know, Boomer Simo coming out, but I don't know. This is one of the rare instances I'm against the Ulti. Yeah, I'm gonna actually make another bold statement to agree with you. I actually don't love the Ulti, the look of it that much. I still like it cause it's cool it's Ulti dad, but like I feel like the Ulti is almost like, it almost like it takes away the card artwork and stuff a little bit. That's how kind of how I feel about it. So if you guys feel, you know, strongly the other way, let us know in the comments. All right, let's move on. That we are now onto the last two questions because nine and 10 we ask at the same time, that way you can't like, you know, maneuver any tears or whatever. But so far you've had three tier threes already, three tier ones and two tier two. So you're guaranteed that one of these is tier two. It can help you out there. And I've got three lifelines, baby. All three lifelines.  So let's do this. All right, Simo, since you are a ghost rare hater, as I say. Yes. I decided to end it with two ghost rares episode or question nine and question 10. Question nine is black rose dragon, crossroads of chaos, ghost rare. This is the unlimited copy. Unlimited, interesting. That's like the first one. First ever unlimited. The number 10 is galaxy eyes photon dragon, photon shockwave ghost rare first edition near mint. So you have two ghost rares, all of your hate, like let's hear a little bit. Come on, defend yourself for hating ghosts. Well, first of all, I can't tell which card is which because they look exactly the same. But yeah, it's not true. A lot of people, a lot of people love ghosts. I am not a fan of this, of this style whatsoever. It's just, it does nothing for me. That you saw my picture on Twitter of the black rose that I took and you didn't think it looked good. I think the black rose and the stardust specifically are the only two that I approve of. And maybe honest, sorry. I'll take a step back. So you like the old ones. I think I like the very first ones. And after that, I don't think I like them ever again.  Like making them worse and worse. So the beginning ones I'd say are definitely the best. They look really good to me. I don't know. And maybe that is the case. That's just got progressively worse over time. But yeah, for some reason, those three I can appreciate. I still prefer the ulti versions of black rose and stardust respectively. Oh, come on. But again, everyone has their own preferences. And everyone has their own preferences, but they can be wrong.  So anyway, black rose and galaxy eyes photon dragon. So it's interesting we're doing unlimited here because if this was first edition, this would easily be like the most expensive card we've probably done this time. Yeah, a little bit too easy to know the tier on that one. Unlimited though, I think actually a lot more difficult because that I actually probably have zero clue on. That's okay. Galaxy eyes photon dragon. I do have three. I'm probably gonna use all three here. I'll be honest. So galaxy eyes photon dragon, I'll be honest with you. I know nothing about this card whatsoever. So I'm actually going to ask for a new question on. So if I ask for a new question, does it reset both cards or just one? No, this one is just gonna do question 10, the galaxy eyes photon dragon. Yes. So I would like to reset the galaxy eyes photon dragon for a new one. All right. You're asking for a new question. You say, I don't know anything about ghostry as galaxy eyes photon dragon. Go ahead and put in your guess right now and I'll tell you afterwards because I don't wanna give away the tier that it's in but guess what you think it would have been. I'm gonna say that this is like 200. Okay, 200. All right. We're gonna come back to that. We'll come back to that. Remind me to tell you that after we do the answers. Okay.  You decided that you wanna use a lifeline. So if you're getting a new question 10, that is gonna be the illusion of chaos. I knew I was gonna do that on my own. Dang it. After a slight delay where I gave him the wrong question, we will be giving a completely random 10th question because I gave away the tier. I gave away the price, everything. These nine and 10, it's not thrown out the window. He's just gonna have to know because he has a big brain. So here we go. Let's see if this lifeline helped or hurt you. All right. Number 10, your re-question is the Dark Paladin First Edition artwork, Magician's Force near mint. Now wait a minute. I thought this isn't the Dark Paladin from MFC. I thought that's the one from the master. All right, I'll give you a little background. So this is Dual Masters Guide. Dual Masters Guide, yeah. Yeah, they accidentally printed the Dual Masters Guide, put it into Magician's Force's First Edition. Then if you got this card, you were able to mail it to Konami and get the actual First Edition artwork, which is like the one everyone's like, ooh, super rare, because like you didn't actually pull it out of the box. Then in the unlimited, they had that same other artwork, not the DMG artwork. So this one is the actual First Edition, but it was not on purpose. I never knew that. I always knew, I always associated this with the Dual Masters Guide and I associated the other one with the MFC. So that's neat. That's, I never knew that. Yeah, so it's a weird situation where they put the wrong artwork in the set. I wanna say I like improved my chances, but I don't, I think I'm about in the same position I was before, because that is a card I have zero clue with that. We didn't even know it was the First Edition, so. I mean, exactly, yeah. So we're learning something new every day. Okay, this is why we saved the two lifelines for the end and you're making fun of me. Hey, it's working out, I guess. Sort of, except that I had to give you a different card and like mess it all up, so. So one of these statistically has to be a tier two, correct, because we've gone through three tier ones and three tier threes. Statistically, yes, if we had not messed up number 10, so this could be any of the three. Oh, so at this point it's anything. Anything's good. Yeah, so you used the lifeline and because of the mess up, you have now a less chance to get it right. Excellent, I'm glad that I saved the lifeline for the end and made everything worse. Great, okay. I'm going to use the lifeline on the paladin and I'm unable to ask, do I have to ask what tier it's in or do I have to pick a tier and like confirm that? No, you can just ask, I'll tell you what it is. Okay, what tier is the dark paladin? The dark paladin is in tier three. Tier three, okay. I felt pretty confident that the ghost black rose unlimited is also tier three and I can be wrong, but I think even with the unlimited, it's still worth a lot of money just because it's black rose. So for the dark paladin, I'm going to go with $400 and then the black rose is where I'm going to use my higher or lower. Is it higher or lower than 500? Higher or lower than $500, you're using your final lifeline to see if you kind of have an idea where it is. I can tell you that the black rose dragon ghost rare unlimited near meant from Crossroads of Chaos is under $500. Under, okay. Now the question is how far under? I still feel like this is worth a few hundred. There's been the trend this whole episode of these are old cards, iconic cards from the anime, also staple cards in old formats as well. And so people like their shiny cards.  So that sort of helps the price balloon a bit. And let's just see how you go for the ultimate rare. Which is what I do. So, which is prettier. I'm going to go with for the unlimited black rose ghost near meant. I'm going to say that this is also $400. All right, final answers upcoming. This is the end. Simo, how are you feeling right now? About how you did overall? Not very good. Do you want to start with the galaxy eyes photon dragon that I threw away? Sure, I will start with that. That's actually not going to matter what Sierra was cause I accidentally showed you. So, the galaxy eyes photon dragon ghost are, you guys $200. Yes.  This card is $171. Oh my God. So, you were really close. So, not knowing. You were very, very close. Yeah. I guess. All right. I figured I could maybe have traded up on the photon dragon for something a little bit better. And I know the card, but I don't know it very well, evidently. All right. Breaking the bad news, buddy. How bad do we do? Okay. Question number nine, the black rose. This card is worth $265. That feels low to me. Yeah, it is easy. I feel like it used to be 300. It's come down a little bit, I think. Okay. Okay. It's a lot lower than the first debt. I think the first debt even gets up to 1500 sometimes. Exactly. Less than a fifth, like 20% to buy an unlimited. So, yeah. Sometimes those first debts, that's how it works. Yeah. They really don't look that much different either. So, very expensive card. So, I don't know if that's your worst one yet, but it's close, I think. It's pretty close. Yeah. I'm kind of, that's, go buy those people. I feel like that's under value. Yeah. So, what we found today is that there's a lot of cheaper cards than we would have thought. Yeah. All right. Question 10, you replaced the Galaxy S Photon, which you only missed by $29. I will tell you, you missed it by more than $29. You said $400 on the dark palette. The correct answer was $330. So, you're still not too bad. Not as bad as the Black Rose, actually. Yeah, you actually did okay. You're 400. It's supposed to be Illusion of Chaos, $179. We actually showed you the answer. So, we had to kind of do a little bit of an audible, but would you have known the Illusion of Chaos? I'm interested. Probably not. I knew that this card was played. And so, I know the Starlighted, this is probably some money, not a ton. I probably would have guessed like two, 250 on that, in honesty. So, you would have been around the same range, probably. Yeah, around the same. So, actually, I think it did worse from trying to use my lifelines. And you should have stuck with no more lifelines. I feel like you did better before that. Probably. That was the mistake all along. I should have just got, I stuck with my guns. Stuck with my guns. But honestly, you did pretty good. Now, it's time to see the results and see what you actually got and like determine the giveaway for the fans. All right. Simo, you've completed the 10 question quiz. How are you feeling? What are you thinking? What are you thinking you are? Overall, for not having like looked at card prices in years, cause I haven't really had to, I felt like I did pretty good. I completely botched the end. I will fully admit that. That seemed to be a trend. People are doing really well and then the end, they kind of fall off, you know? They mess up a little bit at the end. So, the two at the same time is kind of difficult, so. Even with the lifelines, that made it worse. They were death lines. Yeah, they're bad. You shouldn't use them. Okay. So, the total value of all the cards that we asked was 2,401 dollars. So, that's the total value of all 10 that you did. That was $1. Including the replaced ones. You were off by $496 overall. Not bad. Not bad at all. And then you got a factor and you got $70 off because you guessed in the correct tier seven out of 10 times. So, you get to subtract 70. So, you're at $426 off. You do a little math. 426 divided by 2,401. You were 17.7% away. Which means you're in the 25% tier. So, you're at 100.  Woo! I don't even know how that's possible. It felt like you did so good. That's why I had to double check. I was like, I feel like you did pretty well. But, I mean, honestly, 17.7% with like the variance of how cards move and all that stuff. I've talked about this for pretty much every episode. If you're like within 20, it's really good. So, I'm considering like, I wanted to see how a few guests did before I like changed up the tiers of how the money works and stuff.  You know, something like that. But, you still made it into the $100 tier. You did really well. I basically was the last couple was basically it.  But, I think the tier threes, the tier threes, if you miss those, that's going to really tank your score. The tier threes are really hard. That's, I would recommend future guests, use your lifelines on the tier threes cause that's where you can really have the variance. Or if you just don't have any idea and like you don't know if it's a tier one or a tier three, it could be anywhere. That's where you can really go wrong as well. Or don't be like me and don't use your lifelines and just go with your gut and then you'll actually do better in the long run. Or do that, yeah. Or do that. Just get them all right. That's what we're getting. I would have been like 20 or 30 off that photon dragon if I actually kept it, but here we are. Exactly. Thanks for having me buddy. This was a lot of fun. It was awesome. Thanks for being here man. Yep. I'm happy to be the worst person on the show thus far. I will wear that badge proudly. You know, probably three, tier third. You're still like the bronze medal. Not bad. Not bad. We'll see, we'll see 10 episodes down the line if I'm still in last. Yeah. I don't think so. I think someone's going to do worse than you. This was no auction series, but this can be the sequel. We'll have to see you guys. I don't know. Maybe auction series will come back. We'll have to see. Maybe. Who knows when we're 35 it might sound interesting again. I don't know. Potentially. Thank you guys for watching. If you want to enter that giveaway for the $100 giveaway, make sure to like the video. Subscribe. Leave a comment down below. And Seymour, thanks again for being here. Got any final words? No, thanks for having me.  Just saying. Just saying. Could have happened. It's my fault. I'll see you guys in the next video. Peace.


Hello traders at CMC Markets, this is Trevor Neal of ROG Research. I'm focusing to you on the 20th of December from London. Today we're going to look at the Nasdaq, the FTSE and the DAX and also we're going to look at the Pound and the Euro. First the Nasdaq is an hourly chart and I'm very concerned about this. We've got a nice uptrend that's for sure but we've got a wedge a pattern so we've got higher lows in place and then we've got higher highs in place which would normally be bullish but we've got this tightening up we've got this after an impulsive move this loss of momentum in this phase in here you see it here in the very clearly in the MACD also in the RSI here so we're definitely losing momentum and it's quite unusual for these patterns to break to the upside but it's literally got a break in the next hour if break either up or down I say it's unusual for it to break up but we've got a clear point to tell us that it is breaking up when the this RSI line is broken more likely statistically to break to the downside and if it does then we should come back to at least around 16,350 so watch out I think given the tightening up pattern that we've got there it's becoming a bit like a coiled spring and it's about to make a move could be up but I think more likely to be down and that would break the trend that we've had over the last few days bring it to a temporary end I think it is just a temporary end of it so watch out but it's going to happen very imminently now the FTSE is in a gentle uptrend it's very poorly performing on a relative basis but all the soft markets are going up even the weakest ones here but this is actually the one with the best pattern maybe the Dow as well but here we've got a nice uptrend line with several points on it we've held the line to get tested it and held it looks as though we're on our way up now it's very cautious about the DAX that turned out to be right the bearish divergence so remember this for the for the NASDAQ we're in similar condition right now turned out to be a good warning so high high high lower high pattern here and also replicated in the RSI but you can see now the RSI has been broken so I'm actually with due reverence I'm going to delete this line because it's now been broken this downtrend line and I think it's behind us now now we're on our way up and now moving to the currencies the the dollar is having is going a little bit steadier that's bringing the euro and the pound down in the south westerly direction in this weakening quadrant is weakening because the dollar is strengthening and here you can see the euros behavior here but what you can see really quite a strong last big move here in the pound this is now the sampling and then the last hour dropped away very sharply is a big gap between the sample and the previous one here so you would expect to see a plunge that would be what a plunge would look like so let's go and have a look we'll look first at the euro so here is the euro weakening we've got two tops in place at one nine ninety and then we've got further resistance up here at one ten oh seven we've got support I should put this in here at one nine fifty and then really strong and important support at 10890 the MACD is reflecting that easiness there the RSI has gone down all the way down below 35 and ticked up and here is cable and it is the plunge that's a big gap on the RRG and the samples there and look what it's done to the RSI plummeted RSI and here the MACD was already on the way down here it's approaching support however and the support is at 126.30 if that gives way then we could really plummet down to around 125.40 it is support however and we have to look at the behavior as it approaches it if it bounces then I think we've got a range in place here with the top of which is 127.60 and then two highs up in here at 127.90 thank you very much indeed everybody I hope you find this useful have a good day and may the trend be with you goodbye


They're testing things upstairs, I think. So while they're trying to get that set, I just want to thank Elise for organizing all this and giving us the opportunity to summarize what we've been working on for the last several years. And we're going to shift gears a little bit. Mark and Manolis gave you a great sense of what you can do by taking all the data sets that the different labs have been generating and how you can make sense of it. I'm going to tell you we're going to get down and into the trenches here and talk a little bit about what data really was generated, how it was generated, and some of the things we've learned about it. So if I can move this up here, maybe not, we're back. OK, maybe? Yeah. All right, so I'm going to try to tell you about what we're learning about the transcriptions of both fly and worm. These are efforts that have involved basically long and short RNAs from both flies and worms, from our group involving several different labs, Fabio's group, Sue Selnicker's groups with Eric Lye and Brian Oliver, and analysis helped with Manolis and Mark. So the idea of both worm and fly genomes were reasonably well annotated at the start of the project. But for instance, in worm, about half of the transcripts really had no experimental evidence in 2007, despite the very significant efforts at capturing cDNAs and directed studies by both Mark Vidal and Yuji Kohara and others. And so our idea was that we were going to do RNA-seq proteomics and some directed studies as well on a whole variety of different stages, figuring that some things had been missed because they only occur at particular times, or they're pretty rare. We wanted to look very deep. We also wanted to be able to capture particular cells and tissues from the worm. And for that, David Miller has been working on methods to isolate fluorescently labeled cells by fax. And then we've been making our RNA-seq libraries from them. And similarly, Sue's group in flies looked at, again, RNA-seq across development, various times in development. They've looked at cell lines. They've looked at particular tissues. They've chopped off heads and pulled those and done different conditions and looked at RNA-binding proteins. And just to give you, we're now trying to see what we can do in context of the landscape of the transcriptome compared to human. And so we're also trying to work with Tom Jingeris. They've collected a massive amount of data from different cell lines. You can see down here that represents a billion reads for each of these different cell lines under a whole different bunch of conditions. And so there's a huge amount of data available in human. In terms of what we've actually produced in worms, this gives you an idea. We've looked at 106 different samples from embryos, replicates from samples taken every 25 minutes apart, different other parts of the life stage. We've also looked at four different species. We've treated worms with pathogens to see what kinds of things can be induced under those conditions. And with David, we're looking at different tissues and cells. Similarly for fly, cell lines, tissues, treatments, poly-A tail enrichment, the developmental time course. Whoops, that I didn't do. And just lots and lots of data. You can see Sue's group has generated more than 12 billion reads here. And I don't know how many nucleotides that reads. What have we learned from all this? Well, what we get from the RNA-seq are parts. We get splice junctions. We get coverage of exons. We get, in the case of the worms, we get splice junctions. I mean, splice leaders, poly-A tails. And then we have to put them together. And sometimes it's fairly straightforward. Here's an example where the gene structure is pretty simple. There's just this one alternative splice. There is an alternative five-prime end as evidenced by different splice leaders in the worm, and then different three-prime UTRs based on the poly-A, different poly-A signals we get. These are the intron. These are splice junctions, basically. And so we can reasonably put together a model like that. But sometimes it gets harder, where you get too many different signals. Trying to put all these pieces together gives us some uncertainty about really how many transcripts there are. We'd love to have technology that would give us millions of, or billions of, three to five kb reads or something like that. We could actually get this straight. But this is from Fly, from Ben Brown's work, where they're trying to sparsify and yet represent all the different signals that they get. And then you come up with really hard ones, and we're doing our best guess as to what's going on in those. Underlying this, of course, we have evidence for each of these different elements. It's the commonatorics that we only can infer. But with this kind of thing, we've been able to significantly improve the gene models. Here's one where we started out, with genes going four different genes in worm base. With our data, we see more than one transcript for this, and more than one transcript for this, with alternative five prime ends. Here, for instance, whoops, I lost this slide. No, I'm all right. OK. Anyway, so what happened is that these two genes actually merged, and these two then in turn merged with that one. And this is well supported by the underlying evidence. So instead of four genes, we actually end up with two in this situation. Another example here is something we saw both in worm and fly quite a bit. The gene model started out, most of the body of the gene was OK, but we found additional five prime exons, or three prime exons, in this case here for one of the fly genes. So significantly altering what you think might be the promoter region. We also find long-coding RNAs. Here's an example from the X chromosome on worm, where this block here represents the longest open reading frame we could find in this gene. It's about 4KB altogether with splices and some evidence for alternative splices. And so we've been finding and adding to these kinds of features throughout the genome. So when you take all that together, where do we stand? So with the worm, these were the numbers that we started out in terms of the different elements, and we're pretty confident of those. Worm base had 105,000 splice junctions. Only 70,000 of those were supported by evidence. We now have 131 splice junctions that we've incorporated into transcripts. We have more if we look for rare splice junctions, but we're ignoring those for now. We have a lot better sense of what's the start and stop of genes. We have lots more exons. And in particular, we have added to the gene count. We suspect a lot of these are non-coding RNAs at this point, but there are multiple genes that appear to be coding from the mass spec data. And we've increased the number of transcripts some four-fold. Similarly, in the fly, you have another comparison there. Again, more splice junctions, more exons. And again, in this case, about three-fold more transcripts than were represented in fly base. All this has changed the landscape, our view of the genome a bit. Here from fly, you can see that this is the intergenic distance represented in fly base 5.32. And this is the intergenic distances after taking into account the modern code data. So we're encroaching and reducing the intergenic space. Similarly, if you look at the worm, if we look at the number of transcribed bases, it was 28 million in worm base 230. We've got about 37 million bases with good evidence of transcription. So that's changing things considerably. Now, can we compare this worm and fly and look at this landscape now with respect to human? And so we're going to use human gen code, which is a combination of Havana and ensemble. We're going to take the modern code data from June 15th and the worm data from the same time. And so the number of loci worms, oh, PowerPoint has encountered a problem. See, that's too bad because it was going to be a pretty good punchline. Worms are going to have more genes than people. Let's see. Let's hope this works. All right. Sorry about this. I'm sure this is coming off my time, too, right, Elise? Let's see where we are. It's slowly waking up. OK, we're about, I got about the right place here. We'll back up a little bit. It did it again. Oh no, here it goes. So protein coding genes, it looks like here anyway. Worms are slightly ahead of humans. Flies are down still about 15,000. These are estimated protein coding genes. But humans have still lots more transcripts. The transcript landscape for worms and flies is increased dramatically, but humans still have many more transcripts. And in terms of exons, humans have many, many more. And from Tom's work, they have evidence for some 40,000 additional genes, 94,000 other exons and 73 other transcripts. These are probably mostly non-coding RNAs of various kinds and at various levels. But this is the comparison with the current view of human. In terms of numbers of long non-coding RNAs, you can see, again, human has many more. And so there's still considerable difference. And in particular, well, you could worry about those kinds of comparisons because we're using different methods and we're trying to put together things in different ways. But here is the human pseudogenes and versus the fly and worm pseudogenes processed in the same way. And you can see humans have a huge number of processed pseudogenes. We knew that.  Worm has maybe 1,100 of these. Fly has about 500. So a huge difference in the transcription landscape between the three organisms. OK, so I want to give you a couple anecdotes at the end here about what we have been able to do with this. As I mentioned, we've been collecting data from different stages of the lifecycle for both worm and fly. And Jessica Lee in Stephen Brenner's group, working with Stephen, asked the question, can you find genes that are associated with specific stages in one organism and see if those are also, are they associated with particular stages in the other organism? And so to start out, you have to find these are the different data sets that were available. And to start out, Jessica defined the stage-specific or the stage-associated genes and then looked across different stages within fly. And it's not surprising you get a nice diagonal. And adjacent stages share some of those same genes. Now, these are just looking at the orthologs, of course. For worm, you get a similar picture. Again, a strong diagonal. And then so the question is, what do you get when you compare worm with fly? And quite satisfyingly, there is a strong diagonal here. Especially through the embryonic stages. Now to make sure you understand what we're talking about here, I want to explain how Jessica got the significance of this one score. So she started out, as I mentioned, looking at worm fly orthologs for each organism. She then looked to see which genes could be associated with one stage or another. In this case, this particular stage had 762 genes that were associated with the 12-hour embryo. And worms had 363 genes associated with the dower. And then she looked to see how many of those were shared between the two sets. Here it's 107. And then used the hypergeometric distribution to determine the significance of that. How likely would you get this number by chance? Importantly, she applied a Bonferroni correction because this is a 36 by 30 array. She basically increased the p value by three orders of magnitude and still got very highly significant values. I want to come back to this one then and look at this a little harder. And I want to point out these two regions. And we didn't see this, if you remember, on the fly, against fly. But when we look worm against fly, we actually see genes that are associated with late embryo also appear in the pupae. And what we think is going on is that there are genes. Now remember, these are orthologs, not just one to one orthologs, but many to one and one to many. And what we think is going on here is that fly has taken a bunch of genes that are important in this aspect of development, duplicated them. And so when we use worms, they have a single copy. And we can see both copies when we look against fly. And so it says that this element looks like there are indeed expression patterns that are shared with orthologs. And indeed, they maybe reiterated in development here, something we did not see by itself. OK, so Mark mentioned that you can take histone marks and correlate that with transcription levels and do very nice predictions of gene expression, and not just in worm, where they started, but with human and in fly. And so we're now at a stage in the project where we're getting a lot more data. Mike Snyder tomorrow is going to tell you about the data on 110 transcription factors. But we would like to be able to get still other kinds of expression data that would limit the kinds of hypotheses that you could make and see if there would be even stronger predictions that could be made from it. So one of the avenues that we pursued is with David Miller looking at individual cells. This is an example of two neurons from the pharynx. So what we're sorting on are just the two NSM neurosecretory cells in the pharynx. And you can dissociate the worm with STS and pronase treatment and up with cells. Here are some examples of those. And if you sort on those and do an RNA-seq experiment and look for enrichment, these are the top 10 genes that are enriched in this neurosecretory cell. This is a serotonergic cell. And these are some of the genes that you would expect to be involved in those. And these are here. So Mod 5, TPH1, CAT1 are here. And these are very highly enriched. Remember that these are two cells out of 1,000. And so this is about as much enrichment as we should expect. These are probably just because these genes are so hard to detect. The signal is so low in the whole L1 stage that the signal just, it's probably noise. Anyway, so that's one approach. We're doing this on a variety of different cells. I think if we can do this on two cells, we probably can do it on one. And so we should be able to get signal one cell at a time if we can do it, if we can find the right labels. And the other aspect that we're doing that we'd like to be able to bring to bear on this, it's praying on mice. Oh, there it goes, is to look at movies. So we're using these confocal movies to see if we can get gene expression at the single cell level. Each nucleus is labeled with histone GFP. And I don't know, this is about 28 cells or something. So these are confocal stacks. We take a stack every minute. And we're just showing you the movie. We don't ever actually look at things this way. But in the same strain, we have introduced a red fluorescent reporter. And you can see it coming on here and labeling these other cells. And because the worm has a constant lineage, and we can follow all this, and Zhirang Bao and John Murray have done amazing work with all this, the idea is that now we have a picture of what genes or what cells are expressing this gene at any moment. And I'll just go to the next slide. And basically what we can do, this is now the C. elegans lineage, not a cluster diagram. And you can see the individual cells expressing. These are they here. And we know what lineage they came from. We know the anatomy. And so the idea is, can we begin to intersect this information with the transcription factor information and other expression data that we've been getting from other things and begin to think about how all these things interact to create a regulatory network. And I'm going to let Mike talk about that tomorrow. In the last couple of minutes here, I just want to tell a story from Eric Lye and Sue Selnicker about three prime ends. And basically, when you look at tissue-specific things, expression patterns, you see that the proximal poly-A sites are used in the testis. Here, a medium poly-A site is being used in ovary. But if you look at the head, there's a very long three prime UTR, in this case, 18 kB. And this is only really used in heads. And they went ahead and actually did a northern analysis on these to confirm that the heads for these different genes all had significantly elongated three prime UTRs compared to other body parts. And testis was generally short. I'm not going to go through all of these, but you get the picture. And they then also did in situ, where they took probes from the whole message and compared it to the probe from just the extended three prime UTR. And these are all neuronal specific. These are showing different patterns. For instance, these spots here are gone here. So these are long UTRs that are specifically used in the nervous system. And presumably, these are excellent targets for microRNA regulation. This just gives more of that so that you get a picture of how long they are compared to the rest of things. And indeed, the longest ones are way out here, and they're all neuronal specific. So that's an unexpected finding from all this data. So I've told you that the worm and fly genomes, the annotations are much more complete. They're much more accurate. And unfortunately, for the worm investigators, they're much more complex. The worm and fly stages do share common stage associated genes, and we're anxious to see if we could possibly correlate this with mouse. Human is going to be another challenge. But do these same sets of genes, are they echoed in mouse embryogenesis? We hope that the expression data combined with other modern code data can begin to predict expression in a serious way. And then I ended with this little vignette about neuronal specific alternative polyadenylation and long UTRs. And as everybody else has done, we have a long list of collaborators. This is the People on the Worm transcriptome project. These are the people involved in the fly. And I think that's it for now. Thanks.  Go first on your left. Great presentation. My question is, I mean, it was pretty striking to see these different lineages from worm coming up. I mean, the gene expression coming on in all those different lineages at the same time. So the obvious question is, is it some extracellular signaling that sends a message to each of these cells and they're all responding? Or is it that each of them is programmed to independently come on at exactly the same time, creating this resulting sort of uniform structure? So that was that particular example. Susan can talk more about Faw4. We certainly see other transcription factors that come on over different times in different lineages. For Faw4, I think that's thought to be cell autonomous. And so these should just be all coming on. Is that fair? OK, so a little bit of both, basically. A little bit of both? OK, fascinating. Wow, thanks. Ben. Hey, Bob, two things. One, I just wanted to credit Nathan Bolli, who is here today for the fly annotation and it never ever would have happened without him. Must thank Nathan. And two, sorry, Nathan. Two is with the cell sorting, is there a concern about RNA degradation during the sorting process? I mean, how fast are you treating with RNAs inhibitors? So all of these are concerns. I mean, the cells are viable at the end of this process. You can plate them out and culture them. We don't do that. We take them and harvest them. But it's true that the cells are dissociated and then put through a fax machine, unfortunately, room temperature. And all this takes time. They're then plunged immediately into trizol. So that while they're alive, we don't know what's going on. But we are at least encouraged that among the things that are there, they make sense. So have you tried doing a time course where you literally just sort slower, do things slower, and see if you get big changes? That would be a good idea. Bob, I was struck by the low exon count in the drosophila genes. I'm wondering if you think that relates to the low repeat density in the eukromatic arms. We have noticed that the fourth chromosome genes where we have a 30% repeat density have an average of 6 exons compared to an average of 3 exons in the eukromatic arms. I don't know. Nathan or Ben, you want to? That was the slide you sent me yesterday. Rapid evolution here. Is that right? I put the wrong one on the bar chart. I thought I got it off your slide. OK. Sean. Bob, I really appreciated in particular the color development. It's very nice. Is that from your lab or from Fabio? No, no. The development is all from the movies and so forth. Oh, no, that's from John Murray is in the audience. He probably took that movie. Very beautiful. Then I have a question. With exactly the same method, we have been annotating for 10 years 50,000 genes in human and about 20,000 in worm. So we always had this big factor. Now, are you sure that you are counting 20, 20, and 15 with exactly the same method? So as I said, when we compare worm and human and fly, we are using different methods. We're using the data as best we can. And so GenCode, they do it. This is a combination of manual annotation and ensemble. And so we're going to try to dig deep into this and see what we can come up with. OK, thank you. OK.


Hey guys, welcome back to my YouTube channel. This is Daniel Rosal here on to do a quick video today regarding how to check The capacity of a external device that you have attached to your Synology NAS So I've talked done a few videos before about backing up video files And I use a Synology NAS a DS920 plus that was kindly sent to me by Synology for this purpose And it's proved to be a really invaluable part of my my video everything. I've done this video so far So as I explained what I put my best videos or my YouTube clips at least I will Mirror those up to cloud storage as an off-site whenever I can And then there's the second category of stuff and I put in that category stuff like raw footage reels b-roll What else would I say stock footage and those I don't really care about as much and as my bandwidth is pretty small It's not really worth my time pushing everything up to the cloud and paying for cloud storage for it What I do is I just dump this lower grade footage onto a USB hard drive. So I've covered in a separate video how you can do that It's actually super easy. You can connect even an NAS to an NAS But I'm just using a hard drive enclosure physically tethered into the USB port with a USB 3 cable for fast data transfer now What I wanted to talk about in this video is Capacity so my plan is to keep filling these things up and then just put a bunch of hard drives into one of those, you know storage boxes, so It's not for some reason. It's not that intuitive you click on your external devices And this will show up in the top right of DSM. I'm running the latest DSM when you have your hard drive enclosure connected But it doesn't just gives you the product string and the ability to safely eject it Which you should always do but there's nothing there about capacity. So you might think well what about in the Storage manager so clicking on storage manager here You can see that I do get the storage read out But for the actual the NAS itself I've got it's a for 4 bay NAS and everything is running fine And I've got my smart tests running and I'm using you know 600-700 odd gigabytes out of the 2.6. I have available to me But there's nothing about external so where you find that data is as follows You want to go back to your homepage And then you want to actually click into control panel for some reason. It's not in Here at least not that I can find it anyway So click into your control panel and in control panel you'll get external devices And this is where we find the information. So this USB disc one That's actually the USB enclosure that I have connected up here It gives you the location of your shared folder that becomes available When you connect the enclosure to your NAS and here's the important data that I'm looking for I've used 183 gigs out of 915 and it's a ext4 file system. So I've got along a long way to go There's a little bit more info that you can do if you click in it You can actually format it directly within From the NAS which is really useful and you can change around a few settings and you can also check I'm not going to go into the settings to because that's not what this video is about Anyway, that's my that's the quick skinny on how you find out the capacity of a external drive whether that's a you know one of those portable USB drives or a powered USB HDD enclosure that you've got connected you need to go into Control panel then click on external devices and then you'll get your very important readout here Regarding how much of it you filled it doesn't even give you a percentage But you can work out approximately where you stand in terms of filling up that external drive Hope that video is useful and if you want to get more videos from me, please subscribe to this YouTube channel