Hi, it's Fritid. Welcome to Above Life Channel. The purpose of this channel is to inspire your spirit and fill you up with hope. So today I'm going to have a conversation channel where I just chat. So I know sometimes it's hard to discern between whether I'm speaking or our guest is speaking but that's just the way I channel. That's just the way I'm psychic. So that's how it's going to be today. I hope you enjoy it. Okay, so I wanted to ask specifically a few celebrities about what it's like to be famous and specifically the specific question is how do you deal with haters? Like how do you deal with all the criticism that you get and just you just get hammered and nailed by that. So I'd like to interview a couple today in the channel discussion. A panel if you will. And the reason why this happened was because yesterday I was thinking about at Above Life Channel I have been getting a lot of comments which I really do appreciate. Thank you for commenting. That's great. I try to read them as much as I can but I recognize as the channel is growing it's going to be harder for me to keep up with everybody's comments. So just because I don't comment back doesn't mean I don't read them and thank you for them. I do appreciate that. But I've had some feedback that isn't positive which is which is common on YouTube I'm sure and social media but I was just like wow that's like how do people get so up in arms you know it's it kind of surprises me like why would you even comment that if you're so and if you don't approve of something why would you even waste your time but so I don't really understand that like I'm trying to come to terms with some of that a little bit um and so I thought I need to talk who should I talk to about this who would have the best advice will get people people that I've been talking to who are famous who've had you know people attention given to them you know and so how do they manage that so the first person that came through was Robin Williams so I'm going to welcome him today and step forward he's here he's like he has a Hawaiian shirt on he's got a Hawaiian shirt and flip-flops on and one of those hats like uh um I can't think of what you call them but that's cute okay all right uh casual did I interrupt your um your Hawaiian vacation in the afterlife or something he's like he's like he's like it's a message Bridget it's a message do you interpret the message by my fashion choice I'm like yes I do and I do I do pay attention to what spirit is wearing thank you because I'm clairvoyant clairvoyant means the psychic gift of sight which means I see that's how I get information that's my primary source is that so that's the first place I go is visual and so yes so he's saying what does it mean to you Bridget like well seeing you like that it means light-hearted like relax like hey you know no problem like just you know go with the flow don't you know don't be stressing just like vacation just let it go kind of thing is you know light-hearted like take it you don't have to take it and so he was the first person that came in he just popped into my head yesterday when I was thinking about this I thought how do these celebrities do this and I've always had a deep respect you guys for celebrities and how intense their lives must be and I do not envy that and I've been really clear about that I I just feel so much um I feel compassion empathy for them because their lives are not their own like public people and the general public public doesn't mean everybody not everybody's like that but some general public kind of fans and stuff feel like they own them like they have access to them just because they're famous and that's not that's not real you guys but that's such a fact of their lives so I just have such a I just feel for them you know and so Robin the first thing that he popped in that's what gave me the idea to do this video he popped in and he said um people are going to love you the people that love you how did you say this yesterday can you repeat it love the people that love you is what he said he said love the people that love you he said people are going to love you and people are going to hate you love the ones that love you that's what he said yeah people are going to love you and people are going to hate you love the ones that love you he said Okay, so for those that love the channel, thank you. For those that don't, I do not understand why you guys are watching, but hey, if that's okay, whatever, I don't understand, but that's all right. All right, so thank you. All right, so Robin, can you talk a little bit about, okay, he says, well, you can interview the other guest. Yes, okay, so the second person that came in was George Michael. He popped in. Now, these are some of the favorite guys that I liked to interview in the afterlife, that I have a few audios or a few videos and then some of them in the videos. I actually, in the description, read the descriptions people because where it says click more, because sometimes I click and I share an audio from the past that I've done because I've channeled and I've done audios as well, not just video. So check that.  He's like, yeah, I know all about haters. He says, really, really?  And he's like, really? Like just ask me. I'm like, well, okay, so he was just kind of like, mm-hmm. Yeah. Yeah, people can, and basically what he said was people can be mean. I mean, and that's, is that the sentiment as well. So let's say welcome George Michael. And so I'm trying to, sorry if this is confusing, I'm trying to recall what they said yesterday and then just a little bit and then today. So George Michael, welcome. And he leans forward to give me a kiss on the cheek. I love you. I love you too. He's so sweet. Oh my God, he's so sweet. Robin Williams is funny and stuff and sweet and genuine. Little more serious though, Robin is more serious. I got us to be honest. He's much more philosophical and reflective and just like wisdom, wisdom, wisdom from the beyond. He's being funny. Now he looks like the genie in Aladdin. And he was in the movie Aladdin, which I love. I love Robin. Oh my gosh. He put the turban on. He's like, mm-hmm. I need three wishes, Robin. We need three wishes. Okay. All right. So thanks George for being here. Thank you so much. Thank you. Yeah, thank you for being here.  People can be very mean. People can be very mean and it does hurt. He says, this is George. He's saying people can be very mean and it does hurt. That's what George is saying. People can be mean and it does hurt. Yeah. And you got just nailed.  So, but your personal struggles were very public at times. You know, I shouldn't say we're very public. Your ups and downs I think were, I don't want to say hidden because they, because they weren't hidden. But I think the expectations of you, Robin, were of a high standard, a high caliber. And I think more of a mentor, teacher. And that role, I feel like you fit really, really well. Not even as a comedian. Like, I don't have any, I think maybe did you? Okay. So he's kind of, okay, we're having like this dialogue, which is going to be tricky for you to follow, but just kind of stay with me. So at times you felt, did you feel typecast as a comedian? Let me ask you that. Did you feel typecast?  And he says, it doesn't stop. You don't stop. That's part of who you are. Just how you, you know how you talk about, you know, being in the psychic and walking through the grocery store and zooming in on people.  You can play with it. You can, you know, you can have a good time with it. But you have to have breaks. There has to be a time when you can just be a regular guy. And your friends understand that, especially fellow comedians, you can just be a regular guy. But that's where criticism comes in, Brigitte. That's where criticism comes in. It sneaks in. That's what he's saying to me is the hardest critic. Robin, you're brilliant. Robin is brilliant with his advice. I'm telling you, teacher, mentor, great, his advice is, oh, I'm going to get emotional.  Don't you be a hater of you?  I don't hate myself. I have, I think I have a pretty good self-esteem, but gosh, once you start to get bigger, like more people notice you, your videos, and they comment on them, like they're commenting on my videos now. There's some really mean people that say mean things. And I, it just feels negative, like negative energy, you know, coming at you. And for me, I have to just shield the negative energy. I don't really take it personally. I don't feel like I take it personally. I feel like I have a pretty good self-esteem. I have a very strong self-awareness of who I am and what I want to do in the world and what sharing and channeling is really important to me. I love it. I just love it. It feels good. Like it's who I am, you know? I'm a channel. My name is Brigitte. Hello. If you knew my last name, some of you do, you would know it totally relates to you. Anyway, so I feel like I have a pretty decent self, you know, worth, but I also am very aware that I am a part of myself. I have high expectations and high standards. I want to be accurate. And when I'm not accurate or I get something wrong or I misinterpret something that I see, remember, you guys, I'm clairvoyant, so I see first. That's my first channel. That's why I describe so much about the spirit and what they look like and how they're sitting and where they're at because they show up at different stages in their lives, like with Robin when he just did a costume change. I mean, that's important information because you get the feeling. To me, it's all about the feeling, the vibration, the energy. That's what inspires people and gives them hope is this, you know? But I agree. The criticism is just natural, but when we are criticizing ourselves, that's the worst. That is sabotaging. And I'm not at the point where I'm doing that, but I don't feel like that. But I will say, and I think that George can probably relate, George Michael, you could probably relate to this. He's like, oh, now you're talking to me. Oh, now you're talking to me. Robin's going to let me talk now. He's like, oh, now you're talking to me? He's just listening. He's so sweet, you guys. George Michael is so sweet. He's so sweet. He's like, where's your tea? He says, where's your tea? I don't have tea. I just have water in my water. He's like, oh, that's good for you. But so Robin, or I'm sorry, thank you, Robin, for that insight about don't hate yourself, don't criticize yourself. You're your own worst critic, because that's true for a lot of us, right? We learn how to judge ourselves early on. I wish I could just take that program right out of there and just smash that SD card for that. But we all do it. It's natural. So okay, so George, I want to talk to you about this a little bit. I feel like the, this is another piece that I want, and I want to talk to you specifically because I feel like you will totally get this. I feel like for me in doing this channel and sharing these videos for me, my biggest fear or the piece of it that's not appealing to me and sharing this on YouTube and doing this public like this is the piece that's not appealing to me is that from what I've seen people who get attention and become known for their skills or their gifts are also the people that get the most pressure to perform stress upon them, unrealistic demands upon them. And I know I'm not putting the cart before the horse. You guys, I get that I'm not famous or anything like that. But there is a fear that when you, for me personally, with the YouTube channel as it gains momentum, when I have videos that have 1000 views, 2000 views, whatever, I feel like number one, it's a great opportunity to touch people, right? But also it's, it's a little scary because I've already had, I've already had trouble with some disgruntled fans of mine or something getting upset by something I've done or talked about or something. I've had hate mail. I've had a stalker already.  And so how like, you were in the public eye, George, you were totally in the public eye. And then you like, it's like seems like you kind of just stepped way away from that. You stepped way back from that you withdrew kind of. Now, is that because of some of the personal stuff that came out about you and some of the, you know, paparazzi stuff and the, you know, stories about you and stuff like that. And, and how do you handle that? How did you handle that? I mean, is it worth it? You guys, Robin and George, is it worth being, being known, being famous? Is it worth it to do what you love, to share your gifts with everybody, with everyone, because you don't just get to share it with the people that you know will love it. You have to share with everyone and the critics and the people that are, are mean-spirited people that hate it also get to see it. And so how do you kind of returns with that? Like, is it worth it? George, I mean, is it? Was it worth it? He says, I can't believe you're asking me that question. Of course it's worth it. Of course, of course it was worth it. Had I not been a singer, I don't, I, I, I have to sing. I have to sing. And look at all the wonderful people I got to sing with, to be with and all of the things I got to do because I had fame. Because people knew who I was. I had more opportunity to help. I had more opportunity. You can use that in positive ways. And all the struggle that you go through, everybody goes through struggle. Whether it's public in the public arena or the, or in private life, everybody goes through struggle. Everybody has struggle. It's the way that you move through that, move beyond it, rise above it. And I will be the first to admit that I didn't do it very well myself. I didn't, it was difficult for me to rise above that.  But back in the 80s and 90s, that was like a thing, right? Like, that must have been very difficult for you. You know, is there, was there conflict there? Or was it was it just a business decision that you made?  There is no private life. There's no private life. He says, look at, look around you. Everything is public. Look at your social media feeds. Look at what people are posting. Look at what the young people are posting. Nothing is private. Everything is community. I'm not saying it's right. He says, I'm not saying it's right. But it's like public domain.  And was somewhat successful in that? I feel I was somewhat successful in that. But to answer your question about my choice of partners, my private life, my dating scene, that was hard. I had a lot of pressure.  And looking back, I think that was, that was just the way it was at that time. But I think it was a mistake. It was a mistake to try to tone down or be quiet about my love life. And I think that showed through. I think people could see through that. And I don't know if it would have hurt my career if I would have come out fully. But I can see that for me as a person, it was part of the pain that I carried. And part of what led to other choices in my life that weren't so positive. It is really tricky. I'm going to tell you guys, and you can probably tell when I, if you watch my other George Michael channeling, it is tricky for me to translate him word for word. So I do use some of my words. He kind of shares the feeling, and he'll say a few things, but he doesn't like chat, chat, chat, chat, chat. You know, he doesn't get super chatty. Well, sometimes he does, I guess, one of the videos he did. But he says, we're just here to support you.  Oh, they're so nice. Okay, so I have one more celebrity in this panel that I need to ask about this. And I think I will just chat briefly with this person. And then I might have to do his own video because he likes to do that. We have nice long conversations. He and I, and it would be, and Robin goes, Prance, it would be Prance. And George just kind of goes, oh, hey, hey, he kind of leans back like, hey, to Prince acknowledges him. And he says, hi. Hello. And he leans over and Prince comes and he shakes Robin's hand and shakes George Michael's hand and sits down, sits down. I'm George Michael has a little dog on his lap. I'm going to tell you that. I know that in a previous video that I channeled and I talked about his dogs, somebody posted I think that he had like big dogs. But I see a little dog with him. I don't know if it's like his mom's dog or his friend's dog or somebody's dog that's in heaven, but there's a little dog on his lap. I'm just going to tell you that, like a Yorkie or a little terrier or something. It's kind of fluffy. So it's cute. I'm just going to tell you that. So I don't know what he did in this human life, but in the afterlife, the little dogs are cute. Like so the dogs. All right. So Prince, I can feel Prince big time. In fact, I can hear there's like an adjustment and vibrationally in my ear. So Prince comes in, he says, ask me what you're going to ask me. How did you handle fame, notoriety, attention, celebrityism? How did you handle celebrityism? That would be actually, did I just make up a word? That's a cool word, celebrityism. Maybe that would be a cool thing for me to ask people in the afterlife interviews, how do they handle their celebrity? That's a good idea for follow-ups like when I ask, talk to celebrities again. Oh, that's a good idea. So how did you handle the fame? He says, not very well. You think? What do you think? How do you think I handled my thing? Well, did I handle it well? Do I get a grade for this? Do I get an A or B or C? Do I get an F? That's a question, isn't it? That's a big question. So I want you to answer this Prince for me as a friend, knowing that the reason why I'm asking about it is because of haters, because of negative comments, and not because I'm worried about being personally attacked, but it just affects other people in the channel. It affects me, too, energetically. It won't ever stop me. Nobody else is going to make decisions for me for what I'm going to do for my life's work. But it still has a place in social media, especially on YouTube. It seems people can just comment and there's anonymity there. I don't know. It's kind of weird. I don't want to say it shocks me, but I would never do that. I would never, even if I disagreed with somebody or really didn't believe something they were saying, I would never post on their channel. Why would I give them that attention? I just would move along, move along. So here's where I'm at. So how do you deal with the haters? How do you deal with that? Big celebrity, you were a huge celebrity. How did you handle that? How did you handle that? He says, I have to agree with Robin. Prince says, I have to agree with Robin. The worst critic is yourself. The worst person, the person that's going to judge you the most is you. I have to agree with him on that. You're going to judge yourself. You're your own worst critic, as they say. That's true. That was true for me. I mean, just look at my vault. All these songs, all this music. And I kind of ran out of time, didn't I? I would tell you Bridget, do as much as you can while you are inspired to do it, while you can do it, do it, share it.  And you've said that before, that this is art, it's not science, it's not, there's no formula for what you do. It's art. It's clearly art, what you do.  Do it as much as you can. Do it all the time. Share as much as you can. And the haters, dealing with the haters, you've got to pay them no mind. People are going to hate. Some people just have, some people are just angry.  And you just got to remember that, are you really going to talk about that? He says, you got to remember. Really? He's showing me God. He's like, God will judge them. Or I shouldn't say that. I don't, I don't believe that, that the judgment day and all that. I don't believe that. But he's like, God will take care of them. God has to take care of them.  And it's a sad life.  That is not productive time. Criticizing other people is not productive time. You got to keep that perspective. And you got to just not care about that. You got to just not pay him no mind. It's funny, you guys, because I got to be real honest, like, I'm not super upset by the negative comments. I'm just not. I'm annoyed that they, I can't just delete, like, I'm annoyed that people do that and it clutters the feed. Like, I just, for other people too, like, it just taints the energy. It's like, it just dirties the water, you know, I'm like, ew, ew. So I wasn't sure how to handle it, if I should just delete negative comments or if I just leave them up there and have other people flag them and delete them or what to do. But right now I think I can manage it pretty well. It's pretty much in a way the community on YouTube here at Above Life channel, I'm able to kind of monitor a couple times a week, pop in and read stuff and delete stuff too, and block users, which I do. I've already done that several times. Well, maybe not that much, actually, handful. Most of the comments are really good. Most of people's energy vibes are great. And people have really, you guys have really good questions, things I would never think to ask. And I totally appreciate that.  All right. So just keep doing what I'm doing, huh? All right. Hey, thanks for that. Fist bump. I'm like, fist bump. Prince is like, really? All right. Okay. So thank you, Robin Williams. Thank you, George Michael. Thank you, Prince, for being a panel to discuss what it's like to be famous.  How do you handle that? How do you handle the haters? And so I appreciate the advice. And I hope that you here at Above Life Channel have enjoyed this video and got some nuggets of wisdom for yourself again from Robin Williams, George Michael, and Prince in the afterlife. This is Birgit with Above Life Channel. Thank you so much for watching. Be sure to like this video if you do. If you have some comments about it, about some of the content here, go ahead and post. If you have questions, you can post in the comments. Remember, I tried to read them, so thank you for them. And if you never want to miss a weekly channel or a weekly video that I share, go ahead and click the red bell, the subscribe button on YouTube. Thank you so much for being here.


Alright Marines, let's finish up with the pistol.  I wanted to show you that any pistol we give you to take to combat is going to fight just fine. You got to take care of it though, and if something's wrong with it, tell your armor and get it fixed. So general rules, don't take a pistol to a rifle fight. A rifle, if you can. Read the directions. You might just learn something and save your life or somebody else's life. And three, the Marine Corps Order and the Combat Pistol Program 200 rounds a year is not going to get you to master it.


One local organization is using a unique approach to bring awareness to the dangers of sex trafficking in our area. Sarah Winkelman joins us live from the Brainerd studio now our Brainerd studio with the details Sarah. Yeah Dennis one hack and sack woman is using her outdoor wood-fired oven to bake over 10,000 loaves of bread that she then sold at local markets and then took that profit to donate to a cause that hits close to home. I'm being exploited sexually and physically and mentally and emotionally in a harem scheme for his own pleasure. That's Rachel a 2008 graduate of Walker Hack and Sack Akely High School who experienced sex trafficking close to home and today shares her story in hopes that others won't go through what she did. Putting a face on it and showing that this does happen to normal people from small town Minnesota. In order to get the message out one hack and sack woman who is a retired marriage and family therapist started baking bread to raise funds. The damage that sexual assault does to people. It's a core. I would say even a soul injury and it starts with a five step grooming process that can happen when you least expect it. My perpetrator. He was a lawyer. He was four years older than me. He was very attractive. He had lots of female friends. Everybody wanted to date him. Very intelligent, but no matter how smart they appear, this group of women is even stronger and they are ready to stop sex trafficking. It's easy to get riled up and excited about these issues, but it's hard to keep having these conversations on a daily basis. Yeah, one loaf at a time. Hope to continue that conversation of local sex trafficking and that includes education and giving warning signs to help local youth and young adults stay safe. Dennis. All right. Thank you very much, Sarah for that live report from our brainer studio tonight. More information about the one loaf at a time organization can be found on our website at lptv.org. If you enjoyed this segment of Lakeland News, please consider making a tax deductible contribution to Lakeland PBS.


Hi everyone, thanks for joining us and as usual I'd like to begin by thanking our funders, the Chan Zuckerberg Initiative for supporting outreach events by NFCOR. Just a minor detail before I start today's session, the stock will be recorded and it is being recorded at the moment and the video will be uploaded to our YouTube playlist and I will be sharing the link on our website and on Slack so don't worry if you've missed it you can catch up later. So I'm delighted to tell you today that we're joined by Reagan Hayward who is based at the Helmholtz Center for RNA-based Infection Research in Germany and he will be presenting the NFCOR dual RNA-seq pipeline. It's a pipeline that is used to interrogate host-pathogen interactions through simultaneous RNA-seq. There will be time for questions at the end of Reagan's talk and you can either use the chat function at any time today or unmute yourselves at the end of the talk and ask them directly. So thanks for joining us today Reagan, I'd like to hand over to you now. Thank you for the introduction. Right so the structure of my talk is going to be pretty similar to a lot of the other bite-sized talks as in talking about a little bit of background first of all, some mention of the pipeline and then some future directions as well. Something that's important to start with what is dual RNA-seq? From the RNA sequencing it's simultaneously capturing in this instance a bacterial pathogen infecting a host cell and through bioinformatic means we're able to assign the bacterial reads to the bacterial transcriptome and the host reads to the host transcriptome. There are some challenges with read assignment with dual RNA-seq datasets and runs through a few of these on the left hand side. So for example here we have a read which is being assigned to gene A. We're quite confident that we can happily assign that read gene A here. When the read overhangs the gene slightly we're still pretty confident that we can say that this read has belonged to gene A. When we have multiple annotations overlapping and the read occurs within this overlap, this makes it a little more challenging. Perhaps we want to just say that this read is being assigned to gene A or is it ambiguous or do we want to take a proportion of the read and assign it to gene A and gene B. We're going to read multi-maps, multiple genes. What do we want to do in this instance? Do we want to say, do we want to count it at all? Do we want to do a proportion or do we want to say it's gene A and gene B? So these multi-mapping reads are a bit of a challenge especially when we can concatenate the genomes for dual RNA-seq studies. So we've got into an intro species challenges. And this illustration I show on the left hand side is both for host and pathogen reads. And depending on the infection ratio, generally, we have a much larger proportion of bacterial reads in the sample. And so it becomes really important to try and assign as many bacterial reads as possible and as accurately as possible. So I'll spend some time on the next couple of slides talking about the bacterial transcriptome architecture, which I think a lot of people probably aren't aware of. A lot of people are probably more aware of the host side, eukaryotic side, and how splicing occurs during the splicing events. With the bacterial transcriptome architecture, bacterial genes are grouped into operons. So on the right, we have a monosystemic operon with single gene and a polysystemic operon with multiple genes inside. The operons are generally flanked by five and three prime untranslated regions. And within an operon, genes are co-transcribed into an mRNA transcript. In aluminum base sequencing, the transcripts are fragmented into reads and the reads are assigned to genetic features. So this example here, the red reads aren't being assigned, but the blue or cyan colour reads are being assigned to a particular gene. This brings about a few challenges. For instance, many of the bacterial annotations aren't actually complete. If you look outside in the model organisms, such as E. coli, salmonella, and bacillus, a lot of the annotations don't include any of the UTI regions or a lot of small RNAs and even complete genomes. So a lot of the bacterial species would be in just contigs or scaffolds. In addition, a lot of the bacterial genomes contain a number of highly repetitive bacterial sequences as well, although so that can be difficult to assign reads to. So going on to that a little bit further, we've worked out as a uniqueness score per gene in each bacteria. So it's a canada-based approach and we're looking at each gene. So we assign a number of canas to each gene and depending on the uniqueness of those canas, it can be seen in other genes and then we can assign a uniqueness score per gene to each of these dots. If the canas are unique, that means the gene gets a uniqueness score of one. If all the canas appear in another gene, that means that gene would become a duplicate. You can get varying levels of uniqueness per gene. We've got a, so this is dictated by the color, red being a duplicate and gray not. We've got a, we'll set a cut-off at about 50% saying that anything below is considered to be repetitive. So for chlamydia, which is a gram-negative obligate intracellular bacteria, most of the genes are quite unique. If we expand this to other bacteria such as mycobacterium leprae, driptococcus pneumoniae, salmonellatiferium, and orientia, tutu-tsigamchi, we can see quite a difference. So if we look at the contrasting orientia, which is a gram-negative obligate intracellular causing scub-typhus, if you're interested, contains a lot of repetitive elements as you can visually see here. It goes into a table form, for example, so the duplicates are the ones in red and the repetitive is anything below a cut-off of 50% uniqueness. You can see chlamydia has a total of eight genes. It would be challenging to assign reads to and orientia, for example, has over 1600, I think which is about 60 or 62% of the genome. So something to keep into consideration. Yeah, and both genomes also contain a lot of repetitive elements as well, which I think a lot of people are probably quite aware of. For example, the host genome contains about 45% repetitive elements. Human genome about between 50 and 70%, and in some of your RNA-seq studies, if you're looking into different plants, for example, the maize genome has over 80% of transposable elements. So most your RNA-seq experiments typically, we use genome-based approaches such as star and maybe feature counts or HTC, something like that. In the pipeline, we're introducing a transcript-based approach. Within the pipeline, we're using salmon for this. So salmon has two modes. It's got an alignment-based mode, so that uses an existing tool, let's say, star to align the reads, and it'll use that BAM file to quantify. The second mode is selective alignment, which does a pseudo-alignment step in quantification, so it's containing some salmon itself. And one of the advantages of using salmon is it uses this expectation maximization algorithm. It's not just salmon, I should say, other software such as Callisto Express RCM. They use this algorithm as well. So it's going to assist in assigning some of these multi-mapping reads and also reads to some of these repetitive sequences and others through an iterative process. And I'll give you a really basic example. So the first step would be assigning all of the uniquely mapped reads, so ones that have a really high confidence. And then it would go through an iterative process assigning the remaining reads. So for example, after the first step, maybe GNA has zero reads and GNB has 100 reads. And there's a read that could be assigned to GNA or GNB, then we'd have a much higher probability of this read being assigned to GNB. And so using salmon, we've found through a lot of benchmarking, is advantageous for assigning reads from dual RNA-seq data. So we'll talk about the pipeline a little bit now. So some of the inputs, we have Illumina-based sequencing reads and a host and pathogen genome and annotation. We've got FastQC, need for some quality control steps and also adapt to removal and re-trimming through BBDAC and cut-adapt. And some preprocessing steps of merging the host and pathogen genomes and references to create this kind of like chimeric reference that we use. For a parallel read mapping quantification steps, we've got a more traditional genome-based approach which is using star and HTC in this instance. And then we have our two transcriptome-based approaches using star and salmon confinement. For the traditional genome-based approach, you can just supply a host genome annotation and pathogen genome annotation. But for the transcriptome-based approaches, you will need to supply a host transcriptome and the pathogen transcriptome is created automatically in the pipeline. And the pipeline output separate host and pathogen features in various reports and plots to include correlation plots for both the host and pathogen samples per condition. Also a proportion, we also get a number of reads showing the number of uniquely mapped host reads, uniquely mapped pathogen reads, multi-mapped host and pathogen reads, cross-mapped reads or cross-mapped between species, unmapped reads and trimmed reads. And you also get the biotype breakdown per sample as well. And depending on which method you use or if you use a combination, you get this output for each method. So status on the pipeline? Well, there's just a number of performance improvements I need to include. I need to update to the latest template as well, which you'll be doing shortly. And I'd like to include some additional outputs, it's graphical outputs and some data output as well. So an example of that is some WIG files that are separated by the host and pathogen. So I'd like to migrate to DSL2. And probably one of the questions I get asked the most when I talk about this pipeline is, is there support for all JOR and AC data sets? And at the answer to that at the moment, no, it's just bacterial because based on this transcript demo architecture I spoke about earlier. And so we're considering support for viral host pathogen data sets. And so if anyone's interested in this particular bit, I'd be curious to talk to them about some of the features that they'd like to see. So now I'm going to try and include those in my next update. I'd like to thank everyone to Harry, the NFCORE team and community, and the salmon development team as well, for their help. Yeah, thank you.


Hi everyone, Joe for jasmiescasebreaks.com here coming at you with 2021 break king stars and legends Three box random letter break number 15 It's the first letter I think everyone knows by now first letter of their last name that appears in the card so Barkley B cream I build bar a oh And any other letters not represented on this will be randomized to the group So like O'Neill I think is the rare occasion that happens All right, so with that being said big thanks to everyone here And there's all the letters right there Yeah, I guess the crew doesn't want to do any of the leaf metal draft Justin two and a one three times one two and three We got Nick down to Robert and we have some on the site or on personals maybe two and a one three times two and three All right got F down to T All right Nick with F Robert with G Adam with Yavin Jonathan with W Roy with P Anthony with S Jonathan with R Anthony with H Mike with E Michael with M and Jonathan with B Adam with D Nick with J Robert with K Gordon with a Daniel with L and C and Robert with T Sort this by column B Alphabetically We're gonna pause the video. We're gonna allow for a little bit of trading time Also, I can alleviate anyone's concerns if they have letter anxiety if you're like, who does this guy have what does Jay have? Ellen Jordan and James So I'll let you know in the during the trade window when we're paused be right back All right, welcome back. I alleviate was able to help some people alleviate their letter anxiety Help them out, but there are no trades So the list remains the same. Thanks for getting in on this ladies and gentlemen This is the last case that we have unfortunately but We're always on the lookout for for products like this. So Keep your eye on the website for more next time. Good luck everybody. Let's see what we can find it in this breaking stars and legends first one out of the gate Is that NT yeah national treasures baseball and it's Dave Winfield nice five out of ten That's awesome in his college gear W Jonathan nice This one's graded and it is an autograph 10. I don't know if the card is graded but it's Frank Thomas, there you go Robert. I was wondering what what T's Here Thomas is one there is from 1990 Fleer update Beckett autograph 10 nice. I Don't think a single letter has ever swept a case maybe a box Maybe Wow from 1989 Fleer sticker basketball PSA 8 Michael Jordan Nick Stover with J you can still bend and peel right there if you wanted to that's awesome nice Michael Jordan Great box one different flight pattern for these bigger boxes here than the hip-rate boxes. All right Next box next three Another graded card. I see baseball 1962 tops baseball PSA 8 and it's Ford setting a new mark. What did he say? Why he Ford shattered a record established 1918 by Babe Ruth. That's pretty good as he ran his world series scoreless Scoreless scheme the SK EIN some good copy there to 32 consecutive innings The Yankee seven and seven to nothing victory gave him a three-to-one edge in the series It's like it's like using a It's like like a college football as opposed to a pro football Says if they change the the actual That's what it is. All right F Nick Stover. There you go Nick another graded car 1988 Fleer basketball PSA 7 Chicago Bulls's Scotty Pippen. That's Roy with the letter P and the third one here another graded one This time football looks like 2020 Panini mosaic football PSA 10 It is Terry Bradshaw From the mosaic. No huddle said you can see about the disco pattern in the background two out of ten Two plus ten equals 12 Close enough, right Jerry's number Jonathan with B for Bradshaw. I have to look on that three. Good luck fingers crossed Thanks everyone for getting in of course. I appreciate it another graded card PSA 9 from 1989 Donner's baseball Donner's rookie Ken Griffey Jr. How young he looks there G Robert Robert nice Ken Griffey Jr. Not great it We got here basketball piece of the jersey piece of LeBron's jersey fabric reflections Swatch LeBron James Nick Stover with the letter J awesome Oh, yeah, there it is 2005 six NBA Reflections upper deck NBA Reflections basketball One more. It looks like an old baseball card here graded PSA 3 from 1956 tops it's Roberto Clemente That's really cool Clemente see Daniel With a letter C Those facsimile autograph right there looks like that's awesome. There you go Daniel. There you go everybody Thanks for watching. Thanks for breaking with us. I'm Joe for jazzy's case breaks calm I'll see you next time for the next one. Bye. Bye


From Hollywood, it's time now for Johnny dollar Paul Kendrick Johnny over at Eastern Ally Casualty remember. Oh sure Paul How are you seeing any good fights lately price-wise? That is yeah the championship bottle the stadium over in Mulville last week We're there now. I had to miss it, but it didn't miss me Huh the minute Georgie hit the canvas in that fourth round. It cost me 50 bucks Johnny Are you kidding? I've watched that boy come up from the Golden Gloves Well, I know I lost on him to 20 bucks Bailey in the exciting adventures of a man with the action-packed expense account America's fabulous freelance insurance investigator was truly Johnny dollar Expense account submitted by special investigator Johnny dollar to Eastern Ally Casualty insurance company 422 Spital building Hartford, Connecticut Following as an account of expenses incurred during my investigation of the squared circle matter Expense account item 1 a dollar 10 cents camp from my apartment to the offices of Eastern Allied when I get upstairs into his personal cubicle I found Paul Kendrick pacing the floor Sit down Johnny have a drink if you want one. No, no, thanks Hey, looks like you're the one who used a drink. What are you worried about? Don't tell me you've been hitting the company till for big money to bet on the fights Johnny. I'm worried about murder Listen, I'm all ears. How long since you've seen Al Coronado fight? Oh, six months a year maybe but before that when he was working all the local arenas You and I were present every time you put on the gloves. So we knew him when he had reflexes quick enough to Well, do you remember how he'd show off by picking a fly or mosquito out of the air grabbing it between his fingers without even hurting it Yeah, sure He was no metal giant not by a long shot, but he had the fastest eyes and hands they ever saw in a man Right, but something has happened to him something very wrong, and I think I know what it is Listen a few years ago his manager Ricky Malone took out a $50,000 policy on him an Uri So what a lot of managers take out policies on their boys and then get them killed Look Al is fighting again tomorrow night in a small town outside of Joplin Joplin Missouri a little place just across the state line Johnny I want you to be there. You mean has a sort of bodyguard. I want you to see the fight That's all see Al Coronado fight Yeah, but this murder correct I'm having a copy of the policy made and you can pick it up at the Joplin post office general delivery. I'll pour I know I know I may be all wrong This may only be a hunch without a single legitimate reason for suspicion That's why I took a whole week to think it over before calling you That's why I want somebody who knows Al as well as you and I do to Look will you go down there and see him while I will pay the freight Pant your expense account anything you like. Oh, now that's an attractive, but do it Johnny. Will you? Item two another dollar ten back to my apartment a pack Item three one hundred twenty four dollars even plain fair and incidentals to Joplin By your leave Paul the incidentals included a new sports shirt loud enough to startle a whole state of Arizona an extra pack of razor blades and A new toothbrush also item four three bucks flowers for the stewardess who managed to find me an extra bottle of champagne I arrived at Joplin shortly before noon and after checking into a hotel found that by some miracle a copy of Al's policy was waiting for me at the post office a Quick plan said it brings up item five four dollars and a quarter phone call. I thought you said Ricky Malone took out the policy But the beneficiary named is Frankie fortina now. Who's he I don't know yet Well, his address is in New York City. You better look him up. Would you I've been trying to let the address on the policy Was a racetrack bookie joint. Oh, so that's why you're worried. That's one reason Well, if you learn anything about I'll let me know will you I'm staying at the Beverly arm. Okay, Johnny Yeah, call me again. Sure. I was tired. So I had a big lunch that's item six went up to my room and slept I overslept It was nearly nine o'clock when I woke up So I grabbed a cab that's item seven and went out to the arena in the nearby town of Mount Elba The five bucks out of eight I managed to get a seat of ringside and time to catch the end of the last preliminum Program told me I was scheduled for the main event against some local boy named rave Cummings I've never heard of him and I doubt if anybody else had a two-sign ever had I Understood why when he stepped into the ring this kid looked like the rankest kind of amateur strong sure and a good condition but clumsy he almost took over his own size 15 feet And it was no act of fooling opponent either Al when he came in looked as good as ever He gave me a quick glance of recognition. Oh, I'm sure he knew nothing about me except possibly my name At the opening belly came out fast all the old speed and timing were there faint weave and flick out that light But punishing left same old pattern same old Wait a minute those quick left jazz were only landing about one and four As though he touched Cummings only when the clumsy ox having to walk into him Because of his speed out took nothing but a few light ones on the body he kept his face well out of reach Oh, yeah, his timing was perfect, but his aim was terrible Every time he shot out his fist he was three four inches wide What a funny thing happened at the end of the round when Al went back to his corner and remember rave It only tapped him a few times in the body when he went back to his corner and started to sit down He almost missed the stool would have if one of the seconds had named it under him funny The second round got underway the same as the first now was all speed dodging weaving keeping his face out of the way But again, he wasn't hitting his mark And then it happened He missed Cummings wide then practically ran into his glove catching it hard in the cheek and down he watched Why there wasn't enough steam behind Cummings glove to hurt it But Al took the counts. He'd been hurt by that tap on the face Then another thing the second he was counted out his handlers practically hold him out of the ring and back to his dressing room And believe me Al looked terrible his eyes had a strange almost far away look as though that little smack had knocked his brains loose My seat was on the far side of the ring But I elbowed my way through the crowd and back to the row of dressing rooms in a hallway built on the one end of the building Al Al Coronado, I told you on the way up the aisle doc. We don't need you the boys. All right.  This is Johnny dolly. I'm old fan of Al's from Hartford I want to see him some other time. No now right away. Come on open up. I Said some other time.  We're pulling out of this burger and we ain't got time to stand around and talk now look buddy Malone's a name I meld's manager seeing when I say get out. I mean vermouth.  This is Johnny dolly. Oh, no, you don't Brother, that's what you're wrong Hey Al Al Good Lord Al. What's the matter with you? Oh? Hello, Johnny. Yeah, look at me. No, no, I mean straight at me here Al I'm I'm all right. Johnny. You're in bad shape. You should never have fought tonight. Oh That's all right. What are your seconds your trainer Ricky? Don't don't let nobody in after fight. Look Al. Can you get up off that table stand up and walk? Oh sure sure Johnny Then come on. I'm taking you out of here to a doctor easy.  He's got it. You bet. I am Who of yours truly Johnny dollar in just a moment Each Monday through Friday evening most of these same stations bring you the Amos and Andy music hall variety entertainment at its best For top songs informal visits with top stars and for a never-ending supply of fun Turn your home into the Amos and Andy music hall five nights a week Now act two of yours truly Johnny dollar and the squared circle matter When I came to the dressing room was dark and quiet After carefully falling off the table where they'd left me I groped my way to the light switch Stumbling incidentally where the remains of the chair Ricky Malone had used on me It was well after midnight. So I left by the dressing room window The second I reached my hotel room I put through a long-distance call hoping Paul Kendrick would be in home in bed He was Johnny dollar and Paul you're right. It'll be murder unless I can stop it Hey, you awake Plenty and listen that boy is more than punch-drunk.   That's why he kept protecting his face Who else I just met the gentleman by the way Before I could he cracked me over the head with a chair See if the police can track them down Malone said something about leaving town right away You kidding I haven't had time it's an annuity that much I saw beginning on a nice little income of the rest of his life If he survives, but the beneficiary named yes with the policy of al dyes, Johnny Yeah, I got a rundown on Fortina. You said he was a bookie at one time I was the least of his then you're probably thinking what I am you know that yes So with this injury to his brain the only way Fortina can clean up on him is by seeing him dead That's right. Oh, what about medical examinations before these fights? That's possible, of course. Also hardest things in the world to detect. Yeah. Yeah, I must admit He looked great when he entered the ring Okay, Paul one things in our favor either Al nor Ricky Malone knows who I am outside of being a fight fan Just so 14 it doesn't learn different. Where is 14 by the way? Expense account item 9 370 for a couple of phone calls some breakfast and a taxi to police headquarters I'll say this for the gentlemen police when they go into action.  Thanks very much Something and I think it gives us the whole story Johnny Alan is manager Ricky Malone checked out of their hotel the Raybury at one o'clock this morning just the two of them ride There was no third party by the name of 14 or anything else just the two of them They caught the 140 plane for Oklahoma City, huh?  Going down there. I told you I got to save that guy's life All right. Look in Monterey. Look up Sergeant Romelia Garcia main homicide division. You mentioned my name He'll give you anything you want. Good. Now. What about that plane? The deal on plane connections turned out to be bad the best time I could make was by way of El Paso That's item Santa 127 dollars including incidentals I finally pulled into Monterey shortly after 8 p.m. I part my bag at the airport taxied into town item 11 I went straight to main headquarters of the Pulisier Sergeant Romelia Garcia was off-duty had gone to the fights Item 12 $4 American for a fast taxi ride to the Plaza del Fistic cops or what a minute call it Therefore item 13 five bucks. I had the sergeant paged over the PA system After two or three minutes a short stocky important-looking figure and police uniforms stored up to the door Your Johnny dollar and that's right sergeant. How are you you Americanos now? What is so important? I must leave the excellent fights to talk with you Huh the possible murder of an American fighter right here in your own ring So what is that to be excited about something that happens all the time? It's because the Mexican fight is more better than the Americano fight So if that is all it is bother me Sergeant Danny Ruskin of the job Why do you not say so at the beginning? Well, you didn't give me much of a chance How is it my good friend sergeant Danny? Well, this is too long. I have see yeah Excellent man sergeant Danny When I have trouble with one of our Mexican nationals who escape across the border and go all the way to Missouri Joplin He's sergeant Danny who? But you have a problem. Yeah a fighter named of Al Coronado But of course tomorrow night he is fighting here and he will lose. Why do you say that come look? Here on the what you call a billboard a picture of the man he is to fight so El Toro negro that sounds more like the name of a bull in him See big man is he not is this picture real 240 pounds in your but Al Coronado only weighs in at 181 see El Toro big man The senior dollar he is a killer our best three men.  Oh so I Showed Garcia my credentials then told him what I knew and what I suspect Till we have proof of this and you are to start what you call a International situation you are not now in your own country, you know Still he agreed to cooperate first thing of course was to locate Al and his manager in this city of nearly 200,000 that could be pretty rough, but he said he tried He drove me by the airport to pick up my bag into a hotel and There is the bell hop unlock the door of my room. I got a real break the next door down the hall open Hey kid, how'd you like to bring me up a glass of warm milk? Al? Al Coronado. Oh Here boy, just put in my bags inside and leave the door open Yeah alone sure Hey Johnny H. Yeah, that's right Johnny, and I want to talk to you I used to see you excited all the time up my heart for that. You saw me in Joplin, too Only you don't remember Where's Ricky Malone your manager? Oh, he said he had to go meet somebody. He's always going out Look Al, I'm an insurance investigator. Oh, oh, I got some assurance. Yeah One more fight and somebody's gonna collect it. Oh, no Johnny. That's my retiree money The only one who'll retire on it is Frankie Fortina. Hey, Frankie.  Yeah Here catch this bottle Now look what you did. No, no Al you look what you did You missed that bottle by three inches For the same reason you haven't been hitting well why you have these headaches All right, I'll give it to you straight You've had a brain injury Al One good wallop on that head will kill you and that's just what Ricky and Fortina want No, Ricky always says they keep my head protected. So you must be wrong. Am I well Ricky's good to me Why you numbskull he's trying to get you killed. I Johnny you all wrong. You know the man you're up against tomorrow night. Well, I know his name Well, he's the one scheduled to finish you off Johnny, I Don't believe that Al. Listen, you gotta believe it. Now. Where's it up here? Who you gonna call? Hello, this is an emergency. Get me Sergeant Romino Garcia at Central Police headquarters cops. That's right Al and a doctor Oh, no Ricky says to stay away from doctors all they do all they do is stop you from ever fighting again And that would make you worth just fifty thousand dollars less to Frankie for Sergeant Johnny dollar I found Al hotel room right next to mine room 915 bring a doctor a brain specialist if you can even if you have to drag him out of bed Don't look we'll fight the international situation when we come to it you get a doctor up here.  Huh?    Dollar had better have an accident Fall out of the window perhaps. Oh now wait a minute boys. I mean what listen to me well I had two reasons for coming down here to see if you were right about dollar and to make sure of that fight tomorrow You've been stalling with Al you've taken too long the heat is on up north. I need the dough I told you boys that El Toro will do it tomorrow.  Maybe you can get back to the States, but me with dollar laying dead here And if I'll talk Al won't talk you won't either Frankie Dollar has given us a perfect setup. He came here to Al's room.  I have plenty of them.  You set him up for this El Toro tomorrow night You'll do it Ricky. No, then I'll use the gun on all three of you Frankie How do you mine fortina am I it will still look like a fight between you and Ricky boys I'll just happen to get hit accidentally by the gun that will be found beside your body Boss I'll do it You bet you do anything you say if you just help me get out of it Okay Okay, go ahead Dollar is first and brother if you think it's going to be either the window or this gun dollar So far as you're concerned. I don't care which go on Ricky. Just remember your own life depends on it You bet I Dirty will you the window Ricky the window? I said Remember it's your own life All right, Fortina, so you have got a gun How Yeah, Johnny I I hit him but well, I'll be Season your dollar with one very fine clean left hook. Well, Fortina was watching you and the unfortunate Ricky Yeah, you got here a little late Garcia, you see but tell me senior What makes you think this Al Coronado has lost his punch? Expense account item $1,300 legal expense mainly a deposition for a lawyer to take to court Just now Garcia got me on a having to stay in Monterey for a hearing. I will never know what he did As for Al Coronado, I suggest the company makes some adjustment in his policy that'll permit paying his annuities immediately And why not the company should have investigated more thoroughly before issuing this policy anyway, and if it doesn't show a little heart But I'm sure it will Adam 14 224 50 hotel and incidentals and transportation back to Hartford Expense account total 491 20 yours truly Johnny dollar Oh here is our star to tell you about next week story next week a fast trip to the west coast to an impossible case involving an Impossible man Join us. Won't you yours truly Johnny dollar truly Johnny dollar starring Bob Bailey originates in Hollywood? It is produced and directed by Jack Johnstone who also wrote tonight's story heard in our cast were Harry Bartell Herbellis Victor Perrin Jack Crucian Les Tremaine and Lauren Stubbkin Musical supervision is by Emery go Marino Be sure to join us next week same time and station for another exciting story of yours truly Johnny dollar Stay tuned for five minutes of CBS news to be followed over most of these same stations by the FBI in peace and war Dan cover Lee speaking


Happy Friday November 30th 2018 and not so happy for the folks over at Marriott They have announced today that they suffered a large-scale data breach and it also as a plot twist turns out the uninvited guest was in their network since 2014 so this person has had access or a group of people were not really sure and I don't know that they are either They are working with authorities who sort all this out Once again, we got a big company here that has lost all of our personal data 500 million people's personal data now what the usual suspects of credit card and personal information about you was in there But also passports were among the things in this database along with any other information They had gathered along the way about you staying at those hotels So this is going to be a pretty interesting one to watch because it's an international incident but my Faith is waning in our u.s. Justice system to actually set forth any punishment on these companies because we generally see these data Bridges and it goes back to business as usual But the EU has been stepping it up a bit and hopefully will hold their feet to the fire We're gonna have to wait and see and find out how much trouble they're really in but this is an interesting question I've kind of been researching is have any of these large companies ever gone out of business from a breach like this And after doing a little reading I can't name any large companies now We work with the small business market a lot So we've seen small companies that face these challenges and lose they can't afford the fines They can't afford the remediation They can't afford to just hire a PR firm and put some spin on their entire poor security modeling Now I'm not going after them right away to say poor security modeling after Marriott But their security is obviously going to be under scrutiny And I'm a skeptic to the quality of it when you've had someone wandering on your network for the last four years So it's going to be kind of interesting I'm really hoping and these companies never do a good enough job with this I would love a debrief to kind of know how it happened what preventative measures Can be taken against this and it's also could be a learning opportunity for other smaller companies so we can Become better at security. So anytime there's an instant. I'm hoping for a learning opportunity but unfortunately, we're usually met with some PR spin and Someone will be sacrificing a company and they're gonna blame that person Who will make some type of a golden parachute exit from the company and they will go back as business as usual And their stocks will rebound and everyone will be all Happy except for the consumers who lost all their data and have no real remediation for it And it's just a matter of when it happens again. So leave your thoughts and comments below I'll leave links to the articles about this and the official announcement from Marriott And we'll wait and see how this story develops It's gonna be interesting, but I'm really hoping the EU steps it up and puts these companies and holds them responsible for Once again losing people's data because this is just I don't want it to be a regular weekly headline And unfortunately these companies have gotten away with it and without any real firm punishment against them It is going to continue to be a weekly headline because there's simply no consequences for it security costs a lot of money The breach doesn't cost as much So they roll the dice roll the gamble and we lose in these cases we the consumers lose When these companies don't do things right? Thanks for watching if you liked this video Go ahead and click the thumbs up leave us some feedback below to let us know any details What you like and didn't like as well because we love hearing a feedback or if you just want to say thanks Leave a comment if you wanted to be notified of new videos as they come out Go ahead and subscribe and a bell icon that lets YouTube know that you're interested in notifications Hopefully they send them as we've learned with you, too Anyways, if you want to contract us for consulting services You go ahead and hit launch systems calm and you can reach out to us for all the projects that we can do and help you We work with a lot of small businesses it companies even some large companies And you can farm different work out to us or just hire us as a consultant to help design your network Also, if you want to help the channel in other ways, we have a patreon We have affiliate links. You'll find them in the description You'll also find recommendations to other affiliate links and things you can sign up for on laurance systems.com Once again, thanks for watching and i'll see you in the next video


Welcome to Toffy TV, it is three talking points for the final game of the season today. Space 2, Evidence 2 at the Tottenham Hotspur Stadium. This video was sponsored by One Football, the app that keeps you up to date with all the later scores, transfer rumours and stats, all in the one place to download the app, click the link. Space 2, Evidence 2 today, thought Evidence played very well, said all, I'm going to say, what the game is such in my reaction video so check that out, check that out as well. First thing I'm going to talk about today is Kertruma. Played right back today, thought he'd done well in the main, he's obviously a better centre back, that he's played all season and he's played very well, but he's done a good job for us today at right back. And I suppose one of the priorities is keeping him, isn't it? I don't know how we're going to do it unless the Court of Arbitration manages to postpone Chelsea's transfer ban. There's a lot of rumours saying that's exactly what will happen, it'll go to January and then it'll probably be January to January so therefore basically they can buy you the one thing, the summer and stockpile so that you know to make up for next season, next summer, but Zuma will be one, I think Marco Silver definitely wants him and obviously hands I go miss, but Zuma was excellent today showing his versatility and if we can get him back for next season then it just means that we know what we're doing next season in terms of where we're playing, how we're going to play players that understand the system and I thought he'd done very well in an unfamiliar position today, which obviously show Yeri Mina coming in at centre back as well. I've seen a couple of people saying he's this, he's that, I don't mean to done all right, because shouldn't he's hardly played in the Premier League this season, I thought he'd done quite well, you know obviously when he's up against top you know better strikers than my Fernando Llorente is in terms of mobility, you know we'll have to wait and see but I thought he was okay today but KT Zuma was what I wanted to talk about and the importance of getting him tied down to a contract permanently. Second thing I want to talk about is our attacking football, I thought today there was there's a drop-off without Ratialason and Dominic Calvalumin in the side, just the way that Calvalumin plays. Toten is always going to sniff goals up and he did today because I've had a couple in fact but you know get himself a goal which is great, I'm absolutely delighted for him but we missed the mobility of Dominic Calvalumin to get him behind Tottenham today and to really give all the world a little bit of a headache in terms of running off the back of him, Toten didn't really do that and Walcott doesn't, I know he's scored, I'm criticising two players that scored, Walcott just doesn't offer the same thing as what Ratialason does but what really impressed me was the way we still maintained our attacking impetus you know Bernard dude, although he didn't have the best of games, he's had better, was still a threat and then Adam Ola Llock when came on and he was a real threat when he came on so I was pleased with that and pleased with the way the team is moving forward in that aspect of way you know we've had to make a couple of changes today but we've still continued to press and push on you know we had 19 attempts of goal at Spares you know so that to me says you know says a lot, sorry 17 attempts 11 on target though so if we continue in this vein as we go forward you know I think we're going to do better and better I think Marcel Brans, Marco Silver will be looking at who they can bring in in those areas during the summer that they've already decided but you put you know what's really good is and what's good about good side is they continue to play the same way no matter who's in the team and I thought we did that today, I really did, I thought Luca Dean got up as much as he could, well he was excellent at getting up and down that left hand side obviously Zuma tried to join in that that bit isn't part of his game you know he's playing right back and he's defending essentially which is what he's there for but obviously if we'd have had shame and see what they've been flying down a right hand side as well but really pleased to see the team continuing to play in the way that the manager wants it in terms of getting forward and I thought I thought like you said before we did well today and finally the thing I want to talk about isn't is a matrilator really it's more about what we do with this squad now it's more about and I've just touched on it at the first point so I'm okay to you know and Andre Gomez there's a lot of noise going round in the papers it's normal nonsense that is out every summer you know ever and they're gonna sell isn't this team on this player and you know so far the papers of Solkeen, Guy, Gomez, Dean, Richarlison that's just for starters of Pickford you know six players we're selling this summer if you listen to the the media and it's it is a lot of nonsense you know and I think everything needs to do something about it personally you know I would certainly be having words with you know people from the Daily Mail people from the middle just saying stop writing nonsense I know it happens I know lots of clubs have it as well but it's just I think some of the stuff that has written the negativity that it creates doesn't help us and I don't understand why the club continues to to pander to these people but I think for us even forget the media for now the big thing I think for us is keeping this squad together I think if you can add to the script if you could get if we can get Andre Gomez in on a permanent and we can get Katie in on a permanent and we can add two or three more this side could cause real problems for teams next season and I think we'd be pushing the likes of Manchester United and Arsenal next season I really do obviously there's a lot of variables in that this summer because we don't know who you know is going to stay for instance um he was rumored to really wanting to go in January you know are we going to get Gomez even though the signs look positive until he's there with the sheath we don't know is Zuma going to stay so you know there's three things there that could work out slightly differently um yes that's us brands and silver would have replacements for all three but if we could just keep this team together and build on top of it then I feel we could really have a good go at it next year um because we're a good side we're a good side what we're going to have to do next year though is we're going to have to win more away games and we're also going to have to be able to come now we didn't we've done it today we've come back after going a goal behind got two on in front and obviously we give a silly three kick away near ends up in our net we have to cut those kind of errors out next season albeit it was against the good side today but you have to cut them errors out if you're going to progress and progress but key for me keep this nucleus of player together and build on top of it and if we do that who knows where we could go that is it the final one on the season don't forget to subscribe don't forget to give the video a thumbs up if you want more videos like this 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Hey everyone, this is a special video because we have Mario Kart live, and I'm doing it here with my son. What's your name buddy? That's Aiden. That's right. How old are you? Seven years old the perfect age. I think to experience Mario Kart live now We only have one cart because we only have one switch in the house right now But hey in the future, maybe we'll do an update video do a multiplayer So this is me kind of like a look at it a review I know you guys already know all about this right from last year, but I'm gonna do it with my son I think that's kind of unique. Well, I haven't seen too many videos of people doing it with with children and getting their impressions on it So we're gonna open this up take a look at it There's the back kind of gives you some course ideas stuff like that to start with And hey, here's the switch. We're gonna be using It looks kind of dirty there So we're actually gonna clean it up and put on a new screen protector today as well But you guys will see that I'll do that off-camera. Let's get this thing Opened up. Shall we? All right, so we start on the back here How so the flap opens everybody and then this pulls open like that. Oh boy. Here we go.  So to get started it says to get started download Mario Kart live home circuit So we have to go on here, buddy. And all right, and we have to go to the e-shop with dad's account And we got to download Mario Kart live home circuit game. All right, so search so we can play it. Yeah. Yeah So that's one thing is the software does not come with it You do have to download it It is free to download you can literally go download it right now. You just won't have the cart To play with it. I'm sure someone by now might have figured out a way to jerry rig it But there is a Mario Kart live home circuit. So let's get that started here All right, we got the free download going So let's see here Free download All right close and As you guys will see here it is now Installing all right As Aiden races ahead of me here. So this is so this is the paperwork you see at first tells you how to download it Uh, when the download is complete, you're ready to go. All right. It tells you how to download it We'll set that aside Aiden pull the cart out like you just did So this is the cart. Let's hold on before we grab anything else one thing at a time But let's get this cart opened up. What are you guys thinking here? Oh, oh here we go.  He can't wait Oh, there it is Look at that. So there's our Mario Kart Pretty cool. It's got the camera right here. Look at that. You want to hold that buddy? I know you've been so excited about this. All right Uh, it says to download the game. This is the charger cart. So we are going to get the okay. So this is a usbc Okay, this is the charger. It comes with the comes with a really short usbc cable It hasn't plugged into the nintendo switch dock. We're not going to charge it with the nintendo switch dock, of course Uh, but let's get the rest of this on by anything else or is that it? Nope. There's more Oh, oh You know what these are All right, these are where you set up the track.  This is number four Uh, we got uh number three Number two number one. Uh, and this is how you get the track set up Then we have a couple arrows here, uh as well that we can set up So this is going to be a lot of fun I think the first thing we gotta do is get this bad boy charged and then get building a mario car track and we'll go through how that works and uh Yeah, get playing. So first up. Let's get this charged as aiden and I go try to build a track Uh, we're gonna need some space for this buddy, aren't we? Where do you where do you think we should build it? Over the playroom. Yeah, the playroom. The playroom. All right. We're we're gonna go try to build the track on carpet We'll see how this goes and The playroom so uh, let's zoom on that away. All right. This is the room. We're gonna be building the track in I got uh, mario kart live here. Let's go boot this up That's my son there. All right. Uh, it says press l and r so All right It says Start game or watch trailer. We're we're gonna start game Let's connect your cart. We'll grab the cart bud Just go grab it. It's dead. It's not dead just grab it. Trust me He means it's not fully charged. It doesn't need to be fully charged to use it Uh, so let's connect your cart go to next Please press your cart's ignition button to turn the cart on. All right bud Let's bring it here and let's see what we got. All right Here we got the cart All right, let's see here. Um Ignition button must be this maybe Oh, there it goes There it goes. All right. We hit the ignition button All right Oh, it's flashing hold it hold this one All right. Let's see what it says to do next We did that Now point the camera at the qr code. All right. It says connecting here Nope. Nope. It's good It's updating it bud. It says updating firmware So it says don't touch it set it down It's updating the cart great place your card on the ground. All right put the card on the ground Put a set out on the ground So as you'll see our cart is You know in there All right. See what it says to do Okay Huh? Well, I have no idea what it says gonna do bud. Let's see Oh start your engine. Let's see if it even moves. All right He's knocking Put the thumb up You made i'm here to help you earn your official license to drive a cart Take a quick look around to make sure it's all clear and start driving All right, it officially works Here's what it looks like again on the screen here How to continue Let's take a quick picture for your driver's license All right In front of your face You want to be the face? All right All right. Come here. You want to be the face. Get ready? Don't look at this camera Don't look at this look at the camera smile Look Here we go. Here's the beautiful All right now that my son has his driver's license done Done Congratulations. You're officially licensed Honk your horn. You're ready to learn about gates You know All right, it's not the horn Gates are used to mark your course. Let's set them up together Take out the gates. We've already done that. We've already done that Yeah, all right. It wants to make a course All right place the gates in order one two three four. All right Let's do this together bud All right, why no, no, let's go like this. Let's go. Let's go like this. Let's set them on here. Let's go like one No, no, no, no, no you drive through them. So let's go like two. Let's set two up like right here Maybe so we can be over here over here Yeah, and then four that you just stepped on Let's go like four like right here Okay, okay, I'll try Oh, we want the arrows Get the arrow set up here Like this like this how about like this? How about arrow right here? All right, let's move him All right, so there's our course Let's see how it works shall we? Make sure the gates are facing the correct direction All right Meet me in front of gate one. All righty create course it says Here we go Drive through each of the gates in the correct order. Here we go Got paint on our wheels. Ooh There we go. There's our course All right done I'm trying out Oh, here we go What's happening? What's that Koopa? It's Bowser Jr. The Koopalings followed you here. All right. Here we go. First race Oh, no All right, so we put our first race we get the builder outfits we're gonna equip that We're gonna equip the construction horn We're gonna equip the big scoop All right How do I do all right my son Aiden is now going to play a race here See if you can figure it out. I'm already seven years old So Yep, go around You know how to use an item. Yep, there you go All right, go to the starting spot Which is right there right there. Yep. Now you got it's back up. You got to face the gate All right, so here's how here's Aiden doing his first race And see what's going on here. Here's the car. Let's follow it around the track Uh-oh, is he stuck? Oh, no, he couldn't even make his first turn All right, so here goes my son's first race Here's the car So this is what the course looks like guys now It's going really slow. It looks like it Oh, no back up B. Here's what it looks like on the screen here He's going under the couch Oh, no Yeah, look it's fixed it's fixed Hey, the buddy All right folks, I'm back here by myself. My children are in the under room continuing to play Mario Kart live Hopefully the video turned out. I don't know. We're in that crisp that crisp 4k 60 fps Um, I've done a bit of a rough one getting my kids to understand it I will say that the kids are having a bit of a trouble navigating into getting into the drive I think over time they're going to get used to it This to me is a brilliant product The problem Is always going to be the barrier to entry. So Right now we only have one of these and I heard it's a lot more fun If you have two but think about it, you need two switches So bare minimum you're spending $400 because you need at least switch lights, right? Then you got to buy two of these. You got to buy the luigi one and the mario one That's another hundred bucks. So to have the best setup for this you need to spend at least 600 $600 That is quite a bit of money just to play some ar RC action mario kart It's pricey Now if you already own a couple switches and you want to just go spend a couple hundred bucks I I guess it's not too bad But again, uh, it's just a very interesting product See the RC car looks like it's moving really slow in real life But the intended a great job of making that slowness look extra quick when you're uh When you're actually playing the game and then when you use power ups Whether you're the bullet bill or using like a mushroom or something like that It ends up that the car in real life is going a heck of a lot faster It doesn't just appear faster. It's going faster and surprisingly on my carpet Which by the way isn't like some shaggy carpet. It's you know, it's it's pretty low maintenance carpet It seems to actually work quite well. I know other people have had issues with carpet We're not having that kind of issue. So, uh, this to me is actually really really neat Again, it's expensive to get into and I think the barrier to entry for most families is going to be That this isn't a cheap thing But if you think about some of the other products that have had cardboard involved like this is cardboard involved Like labo labo isn't cheap either So I guess this is not meant for cheap entertainment This is meant for more advanced entertainment definitely meant for kids to use it with parents Or having slightly older kids that could figure it out on their own Um, I have a seven year old and the five year old playing it right now I think it's just a really really neat uh experience and definitely something that uh, we've seen some really creative So in fact, you know, I'm going to show a few of the super creative tracks I've seen so far already on youtube. Uh, you can get as creative as you want with this I I just think it's a blast. You can have up to four people playing at once. I think that's cool Obviously two marios, uh, you can custom theme your marios in the game obviously not in real life Two luigi's, uh, I just it's fun. Is it a full mario kart game? No, but there are cups. There are tournaments. There is multiplayer It is fun and I think for the money It's about as good as it's going to get when it comes to ar mario kart Nintendo you nailed it. You are onto something here. Uh, and this is an amazing experience I hope this isn't the last mario kart live we get I would like to see new karts introduced having a bowser kart Having a toad a peat jet etc. Let's keep building up the kart repertoire here And I I kind of hope that this takes off I know it's probably not going to because it's so expensive and you could just go buy mario kart 8 deluxe And have all the players you want and and just be good. This isn't that but you know what for what it is? Yes, absolutely fantastic product. I like it. I'm enjoying it And my kids are off having a blast right now. I mean Seriously, this is some good. This is some good good stuff right here


I came to Manipa Hospital bringing my wife, who has been hospitalized in Tanzania. She had the spine problem and she was not able to walk independently. She was not even able to stand alone unless she gets supports for nine months. We decided to come to Bangalore. Of course, I searched information about the hospitals in India. I googled and got the hospital and then I decided to go through to read the profile of the hospital. I realized that the hospital was a multi-speciality. It has doctors who are experienced and then we made a decision to come here. My name is Margaret Pius Mbonia. I come from Tanzania. I came here to Manipa Hospital for treatment because I have grounded in nine months. I failed to walk for nine months so I came here for treatment. Since we came here, we were very warmly welcomed. The international patient center were very cooperative. The doctors were actually very cooperative. I really appreciate because they are working as a team and they made their best to make diagnosis of the problem when they realized that the names were very low, I mean very weak. They went further to make the diagnosis of the reason why the names are weak. We thank God they started treatment. Since she started treatment, the improvement is very good. Now as I'm saying, Margaret is no more using the wheelchair. She is no more using the stick. Now she is walking independently. This is miracle. So I really appreciate the cooperation, the teamwork that they have here at Manipa Hospital and I would recommend a person who is having such problems to try to come to Manipa Hospital. I'm sure they are actually implementing their motto which is life is on.


How do I engage with a generation of learners that have so many powerful media tools at their disposal that take so much attention away from the classroom? Video game law at the Allerge School of Law did start out as a conventional law course based on a book in a closed space with the only students in it and the only people who saw it being those who registered. Going open, having a website, when lecture capture came along, incorporating lecture capture in a big way struck me as a way of grabbing the students' attention. The largest part of my career and certainly the beginnings of my legal career and practice, I was a Freedom of Expression lawyer. So even then for me it was all about openness, access to information and being able to express information freely. The fears around how students will react to open or being asked to do things in the open at least in my experience is vastly overblown. The zeitgeist of students is to be in a world of open posting and so having an open course, having a WordPress website allows for that lasting value and it doesn't turn the virtues of learning into something that is cloistered, that is circumscribed, that is narrow. Still sometimes the open tools we have are too vertically inclined. They're about professor-student interactions and we need to create a much better layer of student-to-student communications in the context of a course website. Sharing is education. The whole notion of teachers and students is predicated on the notion that there is knowledge to be shared. So anything that constrains the sharing of knowledge is not a good thing for education and arguably is not a good thing for humanity.


Doing a sorting activity is a great way to help a child learn to compare sizes. You can give the child some paper squares of three sizes, small, medium, and large, making sure there are a few of each size. Then you can say, let's sort these by size. We'll put all the small ones together, all the medium ones together, and all the large ones together. You can first show them how to do it for one of the sizes. Then they can try sorting the other sizes. Learning to sort by size will help improve a child's logical thinking.