That's what we love about the book because as we start talking about validation, we're going to get into this a little bit deeper. It's practical. It's something that you can apply at every level. And you start the book beautifully talking about how this has helped with your dating life, but also with managing others. So can you speak a little bit to how emotional bids and Dr. Gottman's research has helped you in the workplace? Because I think that's the one for a lot of us, we probably think the least when we think emotional bids. Sure. And I'm happy you asked that because I preach that almost more than the relationship side of things. At the end of the day, they're all relationships. I think that's the key point here is you're right. It's not just for romantic relationships. If you're interacting with another human being, they are making emotional bids and they're looking for validation in some form or another. I use it all the time at work. In fact, one of the most pertinent examples to me is I had a coworker who, we can have a history together of having very long-winded conversations where if he's concerned about something, he'll come into my office and I'm not even exaggerating here two hours sometimes for concerns that he has. And we talk in circles and I try so hard to say, hey, we got to cover. Don't worry about it. So he comes into my office one day and he's upset that I've assigned another coworker to a project. And he says, Michael, I just got to tell you I'm concerned. I don't feel like this guy's qualified for this project. And I'm afraid that he's going to mess some things up here. And I did like most people do and I just said, hey, you know what? Don't worry about it. He's going to do fine. It's all going to work out. And if we pause for a second and look, his bid was, hey, see me here. I'm nervous.  You're okay, right? Which is very invalidating. It's turning away from his bid. And sure enough, he just came right back with another punch. Well, I don't know about this. Well, did you do this? And honestly, it almost started to get personal because I said, look, I've got this cover. And he said, well, honestly, Michael, I don't know if I can say this, but I question whether or not you know what you're doing. And I was like, okay, take a step back.  But I realized in that moment, hold on, I'm approaching this wrong, right? I can play this for two hours like it always does, but I had just met with my therapist not a week earlier and we're talking about validation. And I'm like, okay, here's a chance to try this. So what I did is I paused for a second and I just listened to what he was telling me, right? I tried to kind of get in his shoes and go, okay, you know what, he's worried about this. I actually make sense why he's worried because he doesn't have the whole picture.  And then he kind of leaned back and I was like, oh, we're making progress here, right? You know, and so just that validation finally he felt heard and he was able to put some walls down. And so we talked a little bit and after I had validated him, I still didn't agree with him, but I was able to still validate him. And then I said, you know what, I appreciate that. And I appreciate you keeping an eye out for our brand. I think you're missing some information. Can I share, you know, the full picture? And he said, yeah, definitely. And so I explained the situation to him and literally in 15, 20 minutes, he felt good about it. I felt good about it. And he's out my door. And I remember leaning back in my chair and I was like, I just got back an hour and 45 minutes on my life here. Like I was, I was gearing up for another two hour thing here, but it's powerful in management because you're gonna have disagreements. You're gonna have people who are frustrated or upset and they're looking for validation. They're looking for to see that you understand them. And as a manager, as a coworker, it's powerful when you know how to respond that way. When you think about it, all these other relationships in our life where there could be conflicts, well, you can move away from your family. You don't have to pick up the phone if your parents call and you're in conflict.  You could break up. You can move out. But work, we often feel trapped with our coworkers because maybe our career doesn't have that opening to move to. I need that paycheck. I need to make things work. And if you have a toxic work environment where you're in conflict with other people, you don't have these tools. It can really wear you down. Well, and who doesn't want to save more time at work?  That explains why I'm working so hard.


The recent Nintendo E3 presentation started strong, revealing that Kazuya of Tekken fame will be the latest character to be added to the Super Smash Bros. Ultimate Fighter's Pass Vol. 2 DLC. Why did Kazuya make the cut? The short answer is because Nintendo and Bandai Namco have a good working relationship, making this partnership relatively easy. It's important to remember though that relationships between companies are based on relationships between individuals. This particular collaboration was helped significantly by the fact that Smash Bros. Overseer Masahiro Sakurai and Tekken 7 director Katsuhiro Horada are such close friends in real life. In fact, leaving aside both Kazuya and Tekken inspired me fighter costumes, Horada has been helping Sakurai with Smash Bros. development behind the scenes for quite some time. It's no secret that Smash Bros. doesn't always have the smoothest online experience. According to Horada, Sakurai has been picking his brain for quite some time, asking how Bandai Namco has created such a streamlined stable online connection for their Tekken games. Horada has been more than happy to help. He said, I spoke recently with Mr Sakurai who worked on Super Smash Bros. and he showed keen interest in our conversations about online features. He even asked me whether he could use what I said about the ratio of wired and wireless players. Thus, you have Horada's relationship with Sakurai to thank for the improvements to Smash's online service that have been slowly dribbling out. Neither party makes a secret of their close friendship, as they'll often take to Twitter to chat with each other. In March of this year, Sakurai took to Twitter to celebrate the 26th anniversary of the release of the original Tekken on PlayStation.   It's very likely that Sakurai and his team were already working on Kazuya's Smash reveal video by this point, which features a reference to this particular part of Tekken lore. Horada responded to this tweet, simply saying, I recommend throwing them into a volcano. Sakurai then responded again, with a Smash Bros. screenshot showing off this exact scenario. It was a cute joke to share with the world when both developers were already well aware that this would soon be recreated with Kazuya. If there's one thing that didn't help Kazuya make it into the Smash roster, it's the endless barrage of fan requests that Sakurai has sent on a daily basis on Twitter. Horada has made it very clear that he has absolutely no patience for people who harass his friend online, and in fact, has publicly stated on Twitter that he'll do everything in his power to prevent annoying fan requests from ever being granted. In response to Twitter campaigns to get characters including Crash Bandicoot, Sora, and Master Chief into Smash, Horada said, You guys are spammers. I will talk with Mr Sakurai and Bandai Namco's Smash Development team so that your request will not be approved as much as possible. If you are still relentlessly spamming, that just means hampering the work of Sakurai and Bandai Namco's Smash Development team. Just one tweet is enough for request. Do not spam us. Developers don't negotiate with spammer and terrorists. It's heartwarming to see a developer sticking up for his friend on social media, trying to protect him from online harassment and annoying unwanted tweets. While higher ups at Nintendo make an initial short list for Smash DLC characters, it's up to Sakurai himself to decide whether any potential characters can actually be implemented into the game. No doubt this particular partnership was a lot easier than others, because Sakurai and Horada are already so eager to talk shop, share ideas, and even stand up for one another when things get tough. The moral of the story, support your friends. The friendship between Katsuhiro Horada and Masahiro Sakurai is an excellent example of what can be achieved when two people are willing to help each other.


Hey guys, welcome to snow break containment zone and we have a skill preview now of Chen Xing ethereal cloud so skill damage type confirmed is electric damage. Then we have her standard skill, hold to scan the area with the battle lens and mark targets within view. After scanning, launching, spiritual slips, all mark targets dealing electrical damage upon hit and attacking to targets while scanning, Chen Xing's anti-interruption ability is improved when Chen Xing shots are part of any target which is a spiritual slip, is attached, detonates all spiritual slips dealing electrical damage. This one sounds familiar, this is kind of familiar in terms of what it looks like versus Wild Hunt's standard skill. So hopefully we can expect the same type of output from her damage output, I'm pretty much going to be expecting that considering that she is going to be kind of the same archetype I presume but only for electric damage. And next up is going to be a support skill, Scarlet Thunder counterblade, Chen Xing launches Scarlet Thunder up to 5 targets in front, dealing damage upon hit and attacking 2 targets while attached, the Scarlet Thunder intermittently emits pure lightning dealing electrical damage each second when an operative shoots the weakest spot of the target. That has Scarlet Thunder attached, a Scarlet crit passes through all targets once and deals electrical damage. This one is nice to have, this is more or less an anti-mob support skill, so this is going to be very useful in mobs attacking you and hoarding around you. And the kit is really going to cater to a lot of mobs around Chen Xing, that's I think how she is built and how she is going to be used, we're just going to have to wait on how much damage this skill deals, the support skill and also the standard skill. And the last is going to be Dominance, her ultimate skill, Chen Xing combines all spritual slips and Scarlet Thunder to form a Celestial Key which she uses to bombard a large area in front knocking targets into the air and dealing electrical damage after using the Celestial Key. A dominant zone is activated, fear standard continuously strikes in the zone and each strike is dealing electrical damage as well. So again these are just descriptions of what she is going to do, we don't have numbers yet on the actual damage that it is going to deal, but I'm hoping that this is going to deal tremendous amount of damage. That is it guys for her skill preview, so hopefully it's all damage, nothing out of the ordinary, we just have to test her out once she comes out on August 7th. So thank you very much guys for staying this far, take care, stay safe, this is The Warden and I'm out of here.


We're glad to know you're still there and watching the breakfast. We're now going to look at the first hot topic. President Tino Booth sets out fiscal policies to tackle economic realities in his speech. That's what we're going to be looking at, the speech of President Buhari. And to talk with us on that, we have a public affairs analyst, Nick Aghule. Good morning and welcome to the program, Nick. Good morning and good morning to our viewers. I like the way you look. Very patriotic this morning. Yes. Very good. Since I can only see Victoria, I hope you're not changing the name of Nigeria to Victoria. It ends the rear-rear, the same thing. It's not a matter of that. Not at all, not at all. You listened to the speech of Mr. President and he spoke about a lot of issues. Let me get your takeaways from that speech that he delivered before we take them specifically. First of all, I want to thank Mr. President for speaking to us. Because given what we experienced in the last administration, we always heard from the presidency. We're not hearing from our president. We're hearing from the presidency. It is a refreshing departure from that tradition that we had in the last administration that the president would address us directly by himself. So I want to say thank you, Mr. President, and please do more of these speeches to us. One other thing was that I actually thought that speech was going to be on security because Mr. President, together with leaders of ECOWAS, have committed our troops to battle, committed our troops to war. So it is a tradition as well that any time a president is about to send troops of the country into war, he addresses the nations. So that he would tell the nation the reason why our military would be put in harm's way. So I actually thought that was what he was going to talk about, the fact that we heard the outcome of this meeting, ECOWAS meeting, that if the junta in Niger don't step down and restore democracy in one week, that there will be military action. But if that didn't happen, instead, the president spoke on the economy. But either way, please let the president speak to us more often. Okay. Well, so now some of the things, the highlights that that speech brought to us are very clear to us now. And I would like you to comment on each and every one of them if you have the, if we have the time. He is to spend 75 billion naira between July 2023 and March 2024 to strengthen the manufacturing sector, increase its capacity to expand and create good paying jobs. And he's to also fund 75 enterprises with great potential to kickstart a sustainable economic growth, accelerate structural transformation and improve productivity. Each of the 75 manufacturing enterprises to access one billion naira or 9% PA with, let me wear my glasses, with maximum of 16 months repayment for long-term loans and 12 months for working capital. He is also going to energize the micro, small and medium-sized enterprises and informal sector with 125 billion naira. He will spend 50 billion naira on conditional grants to 1 million nanobusinesses between now and March 2024. He will give 50,000 naira each to 1,300 nanobusiness owners in each of the 774 local government areas. He will fund 100,000 MSME and startups with 75 billion. Like you said, everything was about the economy and there are so many other things that I might just continue mentioning to you. But let's just take what you feel about this specifics that we have mentioned. Beginning from the first one of spending 75 billion between July 2023 and March 2024. We are in August. I don't know if this expenditure has started in July because the time frame is from July to next year. Thank you very much for that question. My overall view of the package as announced by the President is that I don't believe in it. And the reason why I don't believe in it is that it's not because the President is being generous to offer packages to the business sector, especially small businesses. But I am worried about the implementation. You see, we have a civil service that is perfectly corrupt. This is the same civil service that the organized private sector gave them palliatives during the COVID era to distribute to Nigerians and the palliatives were looted. This is the same civil service that is looting the budget. And so every year we have budgets, budgets, budgets, but you can't see any economic development. And you actually say, where is the money going? I don't know if our viewers can recall. In the aftermath of COVID, there was a group of scientists in court that were put on television where the Central Bank of Nigeria was giving them money to conduct research into COVID vaccine. As anybody heard about those COVID vaccines since then, not at all, that money has gone. So the whole idea of government giving money to businesses to do X, Y, or Z is not effective because we have tried this several times in the past. There have been schemes like this and they haven't shown any success. What government needs to do is to create a neighboring environment for these businesses to thrive. For instance, we speak about power supply. If you give money, like now they are talking about, we will give 1 billion each to 75 companies. Which are those companies? Who is going to select those companies? What is going to be the spread of those companies? As Nigerians, how do we even know that these companies have collected 1 billion each? What is the guarantee that these companies are not fund by civil servants and public servants? They are all companies that they have funded and collected the money and spread it. So government should not go that direction. Government should create a neighboring environment. 70% of the problems of businesses in Nigeria is lack of electricity. And we keep talking about this thing. We used to have factories that have closed and gone this way. Because we are not giving them enough public power supply. Mr. President, he needs to understand that as we speak today, Nigeria is generating and supplying 3,000 MW of electricity, 3,000. 3,000 MW is what some industrial parts, I'm not talking about his city. I'm saying industrial parts as we get as part. If Mr. President takes his eyes and looks at a place like Brazil, we are there generating and supplying 150,000 MW per day. You ask yourself, if one country is giving 150,000 MW to their businesses and I'm giving them 3,000, why explain my businesses to survive? They cannot survive. So regardless of the money he's giving them, they still can survive because they don't have the necessary energy to survive. And there are other things, for instance, the borders are porous. Businesses try to produce and the stuff are coming in. Enter coming from abroad and being dumped in Nigeria. Enter any supermarket in Nigeria. It will surprise you the range of products that are not made in Nigeria. I was in a supermarket in Abuja here two days ago. I wanted to buy oats. And on the whole range of oats that are sold on that shelf, 90% of them carry Dubai, Malaysia, as the place where they were made. Oats is made from cereal that we should be producing in Bantifu here in Nigeria. Businesses are facing a lot of taxation. Too many taxes, multiple taxes by all the tires of government. Businesses are facing high interest costs. If the interest rate on borrowing is topping 30% or more, how do you expect businesses to survive? Mr. President identified that very clearly in his inaugural speech. And he said that, look, he's going to bring down the interest rate. But under his regime, the central bank also had a monetary policy committee meeting and increased the interest rate. So it appears Mr. President is not even falling up on his promises to us. But he will bring interest rate to single digits. How do we expect businesses to survive? And why are you bringing interest rate to single digits? It shouldn't be on loans that you say you are giving to 75 companies. It should be for the entirety of businesses in Nigeria who should go and assess cheap credit so that they can expand their operations. We have issues with infrastructure. A business produces their goods in Lagos to carry those goods to Camel. Either the truck is going to fall off on the road or bandits are going to seize the truck. You know, so all of those are the kind of things Mr. President should deal with. Once he deals with those kind of things, the businesses will survive on their own. They will swim on their own. They will not even need these handouts that Mr. President is offering. So my view is that Mr. President is failing to deal with the fundamental issues. He's going to deal with symptoms. There is something that is making the businesses not to try. Let him go and deal with it. Okay. Can you just mention them? I know that you've talked about them. Okay, let me leave that. Let's just say, does it worry you that Mr. President is still not very deliberate talking about what will be done to stamp out corruption? Yes, the central bank governor has been arrested and I don't know how that relates to fighting corruption. A lot of things that he has done, some people will say that is fighting corruption, but he has not said anything deliberately, anything specifically as to how he's going to fight corruption. And you just mentioned the fact that there is that corruption which gives you no confidence at all that these policies or the things that he reeled out yesterday in his speech will be implemented at all. So how do you feel about the fact that there is no specific pronouncement regarding how to fight corruption? I feel very worried. I feel very, very worried because Mr. President should actually have a single point agenda. And that single point agenda should be the rule of law. Mr. President should ensure that the rule of law reigns supreme in Nigeria. Once the rule of law reigns supreme in Nigeria, corruption will go. Every one of the problems, the ailments that worry us today, they will go. Because, you see, humanity is meant to be run with the law in those places where our people are jackpotting too. Immediately you arrive in those places. You will see clearly that here is a place of law. You can't just behave as you like. You must behave in accordance with the laws of the land. And if you don't behave in accordance with the laws of the land, you'll find yourself in jail. Mr. President, we raise the riot act to all the security agencies and say, look, my government is a government that respects no passes. I want to bring all Nigerians equally before the law. I'm not just saying this. Mr. President begins to carry out the reforms that will make that happen. Because he's not just saying that. He has to put his money where his heart is. The police we have today, given the funding, given the equipment, given everything that they have, they are going to be incapable. They are going to be incapable of enforcing law and order in Nigeria. Because they don't have what it takes. You remember that the Inspector General of Police. And these are the things I explained, Mr. President, to be paying attention to. The Inspector General of Police, immediately he took office. He said, no moral blocks. No blocks are there. Because that becomes the funding mechanism for the police force. So these are the fundamental issues that Mr. President has to be dealing with. The reforms that we bring about the enforcement of law and order in Nigeria. And once he has that singular objective and he faces that objective, tackles that objective, he will see that he's going to have a different set of people. Because that's what we see. When we get on the flight here in Abuja, right there at Abuja Airport or at Lagos Airport, you see her unruly Nigerians are behaving. The same Nigerians get on the flight. When they arrive in London, they start to behave differently. And the reason why they start behaving differently because in London, the leaders have made the rule of law to be supreme. And that is why you are getting the same Nigerian better quality in London, better quality in Nigeria. Mr. President, I have some other standard. OK. Oh, well, let's just take a final word from you to Nigerians, to those in government, and just something to hold on to until things begin to happen. Here in Lagos at least, the governor has said to help in this hard time, in this harsh condition that we are facing because of the fuel subsidy removal, he is slashing the transport of BRT buses or every bus that is run by the Lagos state government. The price of commuting on those buses is going to be half right now. So at least we have some respite. The question I was asking this morning was what about the people who do not need to go on the buses? How does that help them? Because fuel was something that everybody enjoyed. Now that they have been released or removed, they are now giving specific palliatives. So if you are a car owner or you are someone who goes to work, you have the opportunity to enter the bus. But the person who is at home, who is still going to buy from the market, whose prices have gone up because of this thing, will still suffer and all that. I was just asking that question. But I do hope that it will be better.  So thank you very much. I wrote an open letter to Mr. President to appraise his speech. And his speech has called him very hard on issue identification because he was worked on the money to identify what the issues are. It has called him very low, in fact, 20% of solutions because Mr. President is not providing us the fundamental solutions that we deal with this matter. Although, Mr. President was totally quiet about refineries in his speech. I was surprised because of this fuel subsidy that he has removed. The fuel subsidy came about because our refineries were dead. So you can only come and just remove fuel subsidy without restoring the refineries. I expected Mr. President to be telling us that he has set up a tax force to sell off the refineries so that the refineries will start producing fuel. He didn't mention anything about that. Mr. President mentioned nothing about electricity, which is pinning down businesses. Mr. President is talking about going to import buses. Why Mr. President should be talking about rail? Because why you have a rail line? You see, those 3,000 buses that Mr. President is talking about importing. He said each bus will carry 20% and then there are 3,000 of them. That is 60,000 people nationwide. Do you know that less than all of those 60,000 people? That is how rail is so important. You know, so Mr. President should actually sit down and look at fundamentals. He's not dealing with the fundamentals. I'm not, he deals with the fundamentals. His cosmetic solutions are not going to do very much. OK. Well, thank you so much, Nika Gule, for being a part of our program today. It's always a pleasure to have you on the show. Thank you very much. I'm Algerian, where? OK, that was Nika Gule, a public affairs analyst, talking to us from Abuja this morning. We'll take a short break when we come back. We'll be talking about the power of the right communication. Stay with us.


Hello everyone to today's Bites us talk I'm very happy to welcome again Alisa Briggs from the University of Texas at Dallas and today she's talking about a pipeline called viral integration and off to you. Thank you for the introduction and thank you anyone for joining us at this different time or online later since it's at a different time. So I'll go ahead and get started talking about the viral integration pipeline. Alright, so a quick overview before we get started. I'm just going to go over kind of a background on the viral integration of the concept and then any other concepts that are important for understanding the pipeline itself, as well as the pipeline overview so kind of just how it works what inputs as a user you're going to need to give the pipeline what you're going to get, etc. And then, in addition, I'll talk about some current work that I'm doing with camera agreed so camera agreed alignment is the method that this pipeline is going to be using. It's applicable to viral integration, however, it also has a lot of other interesting potential uses so I'll talk about some that I'm working on, and then future direct future directions and enhancements. Alright, so first things first why viral integration. So viral infection can allow the viral genetic material to be integrated into here we say the human genome but it can be any host genome. And that of course can lead to deleterious mutations, especially if you're getting these insertions into protein coding regions. This is of course pretty common with retroviruses which of course do the retro trans transposition into the host genome. However, it's not strictly limited there is some studies showing that other classes of virus can lead to viral integration into the host genome as well. Okay, so there's going to be 15.4% of cancers that are already attributed to some type of infection with 9.9% linked specifically to viruses. A well known example of this is HPV 16 and 18 leading to cervical cancer. This is, this is why it's important so reliable and reproducible viral integration pipelines can aid in confident identification of either known or novel viral associations with cancer and other diseases. So this is going to create opportunities for us to find methods of prevention for such diseases or possible treatment so again with the example of HPV. The next scene is recommended not just to protect you from the infection itself, but from the cancer that can fall. Alright, so next up we have primary reads. So again this pipeline is going to use a chimeric read alignment method. In order to find those potential violence creation sites so what is a chimeric read a chimeric read or split read means that multiple subsections of that read aligned to different positions in the genome. And this can even be different reference genome so in our case it's going to be a host genome, and then a viral genome. So, in particular chimeric reads I think the most common way you're going to see them or that you've probably heard them talked about is as errors either an amplification or sequencing. So a lot of times, these will just get tossed out, because people think they're just errors and a lot of times they are, but there's also sometimes where chimeric reads can be very helpful to us. They have been used to analyze interact zones so for example, if you have RNA, RNA interactions, you can do RNA seek with those while they are in complex, and you can analyze how they're interacting with each other. They're also useful for identifying structural variants. So these ways are kind of closer to how viral integration is going to leverage chimeric read alignment. So in order to identify those reads that contain a portion aligned to the host genome and a portion of lining to the viral genome. So we have this diagram down here. If you can see the green section will imagine that's like the host genome, the orange section imagine is the viral genome. And then you have these sequencing reads that are going to be overlapping this junction between the two. So if you're mapping these reads, and in this case it's paired in reads, you're going to have part of it aligning to the host and part of it aligning to the virus reference. And that's how you get and leverage your chimeric read. Okay, so that's kind of the background information that you need to understand the pipeline. What about the pipeline history itself. So this pipeline came from an existing middle pipeline created by Broad Institute. This was from the Trinity Cancer Center transcript analysis toolkits, and specifically the tool was called virus integration finders so for short, we'll call this CTAP VIF. And this pipeline was capable of capturing evidence of virus matching reads. So that's consistent with viral infection it doesn't necessarily mean you're integrating virus into the genome. It's just some evidence that viral leads are here. It's able to identify and quantify evidence for viral insertion sites. So this is the integration into the genome. In the CTAP VIF pipeline, it was only able to look at the human genome. So that was kind of one drawback of it, but still super great pipeline. It also produces interactive visualization for evidence of virus mapped reads and virus insertion sites so whether you're looking for just evidence of viral infection, or evidence of viral integration into a genome. It's going to provide some nice visualization for both. And then lastly it includes this nice human virus database so they already compiled a lot of different human viruses into a FASTA. You can align and use that as a reference. This was ported over to our pipeline as well. So if you have are using human as your host, you already have a virus database ready for you. Okay, so when did this conversion start what spurred it. There was an interest in the chimeric read alignment approach, as well as how that could be leveraged to look at viral integration, or potentially other genetic events. So I started this conversion in April 2022. This was my very first step into bioinformatics was the first time I ever really looked at code or did anything related to that. I did this under the mentorship of admin Miller here at UTD in our functional genomics lab. This was a great experience both to have him helping guide me through this process as well as using this existing pipeline and basically learning the syntax of next flow, learning how NFCore pipelines are put together, etc. So this was quite a long process just because this was the very first time I was learning any of this, but the first release PR was opened in January 2023 and subsequently released in March about a year after the conversion started. This is going to be equivalent to the CTAP VR version 1.5, which was released in December 22. So all the scripts and everything are going to be up to date. This is still the current version so everything is currently maintained. So it's this kind of one to one mapping between the pipelines has happened so that we could get to next phone NFCore, where things are a little bit more flexible and reproducible. Now it's time to work on enhancements kind of make the pipeline our own and NFCore is a bit. All right, so this is going to be the Metro maps precursor there is no fancy Metro map yet. I'm working on organizing into subwork flows, and I'd like to do that map when it's all done. But this you can get the general idea, how we're sectioned off right now in these boxes they aren't subwork flows themselves they're just kind of general groupings of the processes. So here we're going to have pre processing and host alignment. So this is where your reads are going to be aligned to the host genome. You actually don't align to the host genome, those get passed forward here and go through some viral read alignment and reporting. And then in these steps you're also going to find your first candidates for insertion sites and your Chimeric reads, then in this this grouping down here Chimeric read identification and reporting, you'll be kind of validating those Chimeric reads, and then reporting them as viral integration events. That's just a very brief overview of the pipeline. So what are the inputs as a user what do you need to provide. So of course with most NFCore pipelines you're going to need the sample sheet in a CSV format. This is going to take NGS reads they can be either DNA seek or RNA seek. Currently the pipeline is defaulted to trim Illumina adapters but this can be adjusted in the modules config file for any other NGS sequencer as long as you have the adapter. So supports both paired and single underreads so currently paired end is going to be supported on the dev master branch with like stable releases single and reads is currently supported on its own branch. So that's also in progress working to support that on our lease. Okay, so those are the reads. And then you also need to include the reference of the host genome so this is going to include a FASTA file and a GTF file. So you will need a database of viral sequences of interest so this is going to be a FASTA file. Again if you're looking at humans. There's already one of these provided with the pipeline from C to have the F but if you're looking at a different host organism, you will need to provide this fast. Okay, so these are the alignment steps. This is kind of where a lot of the meat of the pipeline takes place a lot of the important steps. It's really confusing because there are three different Starline steps. So I'm just going to go through this. So Starline host, as I had mentioned, is where you're going to be aligning your reads to just the host genome. Okay, so any reads that are lining to the host genome and any chimeric reads that we're finding just between the host genome and itself, we're kind of going to push those aside and not look at them any further. You can also output a fast queue of unaligned reads. And this is what's pushed forward into the rest of the pipeline. So those reads are going to go through some more processing and eventually you'll hit Starline plus. So before you hit Starline Starline plus, you're going to have this step that combines your host and viral fastest into one FASTA reference. Okay, and the Starline plus step is going to be aligning your previously unmapped reads to this combined reference. Okay, so of course you're going to get the output of the BAM file as usual with those alignments, and you get this chimeric junction file. So this is a important feature of Star, especially for this chimeric read approach. This file is going to contain instances where you have reads mapping to both the host and the viral reference. And then finally later down the pipeline, you're going to hit a process called Starline Validate. This will essentially take your chimeric reads and where you think you might be seeing viral integration sites, runs alignment again with various inputs and confirms that those are potential viral integration sites. Okay, so this is the chimeric junction file that's going to come out of Starline plus. So the columns aren't quite aligned but we can look at it with the color coding so we have the chromosome donor A, and then the chromosome acceptor B in green here. Okay, so if we look at these first few entries, we see that the chromosome donor is going to be HPV16 and the acceptor is going to be chromosome 18. So this is a good example of where you're seeing that chimeric read, part of it's aligned to the virus, part of it's aligning to the human reference. So where does it go from there? This is kind of the same diagram just zoomed in on the later sections of the pipeline. We're just imagining we start here with the Starline plus outputs that includes your chimeric junction file and your BAM file. So these are going to go into IR support which is kind of a reporting step in the middle of the pipeline. There also should be an error here it goes up into insertion site candidates. And from there you're really analyzing these chimeric reads for their potential to be viral insertion sites. Okay, so you look at their potential, then they come through this extract chimeric genomic targets. And then you're going into the validation steps. Okay, and once you've validated everything there's a little bit more processing that goes on here. And finally you get to summary report which is outputting some really nice visualizations which we'll look at now. So this is kind of the primary data visualization this is going to come out of both virus report and summary report. In summary report it's going to be a little bit more refined thanks to those extra processing steps. So what you're seeing here this is just a bar graph showing the virus mapped reads so this teal color here these are the reads that are being just mapped to some kind of virus. They're not necessarily inserted into the host genome, but they're somewhere in your reads. This kind of pink color these are your chimeric reads. So these are what you're looking at for evidence of that viral insertion into the host genome. There's a difference between the top and the bottom. The top is just run on the full human reference, while the bottom is our optimized test data set where you're just running on chromosome 18 for efficiency. So if you get chimeric reads you're also going to see some dots showing up on this genome wide abundance plot. So what this is showing is basically how many different instances are supporting a chimeric read on a given chromosome. chromosome 18 specifically here, you're going to see how many reads are supporting that there is a chimeric event happening here. So down here it's about 128. For some reason it's, it's about 150 on the full genome run. So then you can also see for the full genome run on 11 and six you're also getting some chimeric reads on those chromosomes. The nicest output that this pipeline gives is going to be this IGV JS. So this is really just an IGV it's just that you're not going to the online web application it's kind of outputting its own self contained file here. And this is going to be showing you basically the alignments and how they're split between the two references. So you have chromosome 11 over here and you have HPV 16 over here, and you're seeing how your paired reads are mapping between the two. So in a lot of instances you'll have one mate of the read pair mapping to one reference, one mate of the read pair mapping to the other reference. But occasionally you'll get these nice reads where they're aligning directly on this junction. So within one single read one, one, one side of the read, you're seeing that it's aligned to both the host and the virus reference. You can zoom in which is also nice feature to get this base pair resolution. This way you can kind of look more at the sequence you can see where there's mismatches occurring. So for instance down here there's a T mismatch or mutation. This is going to just help you have more confidence I think in the alignments. So that's exactly what's going on at the nucleotide level. This is also going to allow you to see how far these chimeric arms extend. So how much of each reference does it cover. And there is kind of some debate on how long should your chimeric arm be for this to be a confident and a significant alignment. Okay, so that's pretty much a short overview of the pipeline what else am I using it for at the moment. The third approach is able to be applied to lots of different topics. One of particular interest to me was novel transposable element identification. So I've been working on looking at how to apply this viral integration pipeline to also look for transposable element assertions within the genome. So it's kind of the same idea you're having some genomic element inserted somewhere in the genome that it technically shouldn't be. It's a little bit more challenging with transposable elements. So my current ongoing effort is to optimize the parameters and deal with the bias towards these repetitive genomic elements, as well as the fact that these are endogenous to the genome, rather than being a viral element which is kind of completely separate from the host genome. The first step was just to place the viral fast with the tea faster. That was easy enough. And then some trial and error kind of led to the conclusion that we should skip the Starline host step. So if you align these reads to the host genome because transposable elements are endogenous. A lot of them, actually all of them in my case, we're getting filtered out in that Starline host step, and we had nothing to work with after Starline So if you skip straight to Starline plus where you're looking at the human reference and then kind of also putting an emphasis on the transposable elements. You start seeing more of your results. And this still needs to be optimized but I thought it was interesting to see how else this pipeline can be used. So future directions. As I mentioned I have been working on the sub workflows those are all laid out and pretty much ready to merge I just need to make sure they're all still functioning properly since I wrote them a little while ago. Also need to NF core I some of the dense local modules so because of how this pipeline was set up. It ended up that there were some local modules that have about 10 Python scripts running in them. Which isn't super module and not super easy to maintain once there's going to start being updates to these scripts and containers. So it would be it would be nice to separate these out into smaller local modules and then perhaps sub workflows from there. Additionally, there's always the battle of optimizing your parameters tests and test data sets as I mentioned the parameter for chimera arm length. That's a particular one of interest. Just to see basically when is your alignment, maybe just by chance or mismatch and when can you start to have some pretty good competence in it. Additionally, some enhancements I'd like to add I've been talking about this one forever but adding metaflan three. That would be helpful. Basically, you can look at the microbial profile of your reads and see what viruses are showing up in metaflan three via those reads. This would help to confirm your viral alignments that you're getting later on in the pipeline. If you look at your metaflan three oh I have HPV 16 and HPV 18 showing up in these reads somewhere, and then you see a viral integration event later that has HPV 16 or 18 that can increase your confidence in those integration alignments. Additionally, I need to add support for aligning long reads. So that'll include adding another alignment method. That would be super helpful, especially for aligning chimera greeds. And I'll continue the development of identification of chimera greeds involving TEs and their respective profiling. And then I love to do more collaboration with this pipeline. So although it's been self imposed, most of this has been a solo effort or a duo effort when I was working with Edmund on it. I would love to have more of the community involved. Here anyone's suggestions and ideas for it. And really just have more of this collaborative effort. So if anyone's interested you have ideas. You think something should be done another way feel free to drop an issue in GitHub or in our Slack channel. I'd be happy to hear about it and work with you on the code about it. Okay. So these are just the references for any of the figures and or statistics that popped up throughout. And that's all. So yeah, again, if you would like to jump on the repository submit an issue or chat with us on the viral integration channel on Slack, I'd be happy to talk to any of y'all. So thank you for coming. Thank you for watching. And yeah, if anyone has questions, I'll be happy to take them now or later on our Slack channel. Thank you. This is not a time exactly for questions. So if anyone. Please ask now. But maybe I can ask one. So why did you choose to star as your liner. I'm a star. Yeah, so there was some question between like star as it's, it's RNA seek optimized right not necessarily DNA seek optimized. But star have that really great feature for identifying the chimeric reads and split reads, along with outputting that chimeric junction file. So as to the entire why it is just how it was in CTAV VIF. So this was kind of a one to one mapping, but I think that's kind of the argument for it as to how well it maps those chimeric reads. I think there's actually quite some overlap with high C pipelines, because they also look for chimeric reads. I don't think they use star at least the analysis that I do is with BWA and then pair tools. So maybe you could have a look into that if you're interested, but it's the same kind of problem because they also look for chimeric reads. More in a genomic context then of course, not transcriptomics. Yeah, I'll have a look at that then. Cool. Are there any more questions. Anyone can now unmute themselves if they want to. But if not, then I would like to thank you for this really good talk. And I also would like to thank the audience that's here. And the John Zuckerberg initiative for funding bikesize talks. So thank you very much everyone. Thank you.


Hi everybody, this is MXUX and this is a presentation on why I think Workhorse is underrated and I don't think it's the post office deal, although I do think this is going to be the new postal van and I think they're going to build it at Lordstown Motors, that's the smaller Workhorse van they have, but let me just go through these slides here and I'll show you what I'm talking about, oh look at that, so here's one of Amazon's drones, they've got many different prototypes out, they spent a hundred million dollars, a billion dollars on research, anyway, I'm giving you an idea of the cost savings here, right now to deliver a package, it's a dollar a mile, that's the cost to deliver packages I guess, it's a dollar a mile for a conventional truck, a tunnel combustion truck, like a UPS truck that you have now, if you move to an electric truck like the Workhorse C1000, it cuts that by 66%, it takes the cost down and that's just changing to electric to 34 cents a mile from a dollar, then if you go by drone, it takes it down from 34 cents to 4 cents a mile and I think that's supposed to be a 96% savings, so the amount that can be saved by implementing drones in delivery for e-commerce and we know that this e-commerce trend that's going on is going to stick after everything's over that we're going through right now, so delivery costs and getting delivery costs down is a big deal, that's why Amazon is so obsessed with it and you know just, they did buy or they did partner with Rivian to build the vans, they got those little plastic vans they're building, I'm not sure how that's going to go, anyway let's move on to the next slide here, this is a video of, they're actually their first very first drone delivery, they got a pilot program that's been working in England, a small, I forget what the village is, West Ham or something, I don't know where it is, but anyway there's a warehouse relatively near the village and they gave them the licenses to run the drone, so they're doing the drone deliveries there, that's their first drone delivery there and that's kind of their pilot program was overseas in the UK and you know they fly off from the warehouse and they go and they drop it off and you know it's a pilot program, it's been working for them, anyway here it is again, alright, let that play, anyway now here's one of their drones, they have, I don't know, the new one looks like a tie fighter, I think this is one of the first first generations they had, but you know like I said the new ones are completely different from this and I think they have a lighter payload but one of them kind of looks like an old B-52, one of them looks like a tie fighter and then this is, just looks like, I don't know, looks like they got a little Bondo on there, I don't know what they're doing with that thing, anyway here's their drone program, they started it in 2013 and they expect to save on implementing drones $43 billion on deliveries, $43 billion, I don't know what that, what is that, you know, the income of Western Europe, gross domestic product, anyway, they spent $100 billion, that's not an incorrect figure, I checked it, $100 billion on research and development and they spent $9.4 million just in lobbying fees to, they had the lobby to get FAA approval to operate in the United States and I don't know what that was all about but anyway they had to you know spread a little money around to expedite this approval because I guess it takes a while and anyway they pushed it, so they got a pilot program in England and it's doing deliveries from one warehouse and it's you know a very simple program and as I said the drones are stationed at a delivery center, a warehouse outside of town and in all of these situations that Amazon is facing, it's the range limits of the drone that's the problem and it means that the warehouse they're based in has to be close quotes to the customers and I think the range, I'm not sure that they're coming up with and I'm sure there's a weight to payload curve and I don't know, you guys, you math heads can figure that out, go for it, egg heads, anyway the drone range limits, let's say it's 15 miles, let's say it's 15 miles round trip or 15 miles one way, whatever, the warehouse has to be close to the customers, if it's any further than that either they can't carry any weight or the drone can't make it back, so they have a problem and all these land-based drone schemes have the same problem of range and you know I guess it's the control signals that go to the drone and everything else anyway, it requires that they be stationed within a certain radius of the customers, so they can't get around it, now Amazon, you know with their hundred billion dollars, you know these are the smartest guys in the room right, they came up with a couple ideas here how to get around this range problem, now I call this the beehive, see this, this is a, oh wait a minute, oh wait, wait a minute, I went ahead, okay there we go, so this is a beehive these are all little landing pads for the drones all right and they got hundreds of drones in here and they got the trucks coming in and then the workers coming in and they they they packed the drones up and and they fly out and then they fly back and there's hundreds of drones flying in and out of this thing right and then this is this is another same thing different shape okay cookie jar you got your trucks pulling in your workers coming in you got all your little drone holes coming out of here it's like you know it's like the flying monkeys in the Wizard of Oz or something I don't know they're buzzing in and out and buzzing in and out and all this stuff anyway again this is how they're thinking they're going to do their land-based drones now here's the thing in this scheme and this former scheme the beehive and and the cookie jar here's the rub they want to put these things close to the customers that means in the middle of urban areas you know inside of town you know down the block you know at the empty empty field next to the middle school so these things ain't going to be out you know out in the middle of the country because that's where their warehouses are now these these have to be closer closer by definition like they're talking about having them right in the middle of New York City and Chicago and anyway okay so we're going to have this thing we're going to have one of these it's going to be in Los Angeles and you know it's going to be right in the middle of Culver City or wherever and you have all these things flying overhead in and out all day long and anyway this is their other other idea they came up with is a blimp this is some Japanese artist came up with this but they're talking about and they got drawings on this I don't know if they filed a patent on this or not but anyway you get the blimp it's the blimp warehouse okay it's the zeppelin approach lead zeppelin approach so they got a blimp so that so in this case they get around having to make these multiple closely stationed warehouses with all the little drone holes in them you know with the flying monkeys coming in that he got the blimp they got this this giant blimp and this blimp is just gonna go over in the neighborhood and wherever they're needed and they're gonna hover there I don't know how high I don't it's not gonna be this close but you know how high could it be right because there's planes and stuff so anyway it's gonna be up there and and it's gonna have all these drones there's gonna people in there loading drones or robots or whatever and this thing is gonna fly over your neighborhood and stop and you know a couple hundred of these things are gonna come buzzing out of there flying around neighborhood anyway this this is a this is a thing I ain't making this up so let's talk about workhorse okay that was Amazon and this is workhorse now this is the same company that is up to do the USPS bid everybody's having a connexion about because it hasn't been let yet and so on let's just talk about their drone program and how they got started anyway this is to 2017 and this this premiered at the Paris Air Show and I mentioned this in my other videos and some big walk analyst was just on MSNBC talking about well you know the next thing Uber's gonna come up with is a flying drone taxi and you know this Steve Burns the CEO who's now CEO of Lordstown Motors this was his pet project and I there's presentations on the web on YouTube you can check them out but one of the cool things about this forever practical the arms bend down and you can park it in your garage or or in your driveway you know what I mean and park your car next to it anyway this is all composite it's kind of a hybrid it's got batteries and it's also got a small gas gasoline engine in it like I don't know to really two-cylinder engine it's also got a one of those ballistic parachutes that will fly out of the top right here I guess if everything fails and the parachute opens up and it lands softly but anyway there's there's double motors okay and so if one of the motors fails the other one and one will work and they got the ballistic parachute and then they got the gas engine which generates the power to run you know it's got two generators hooked up to it to run the the propellers and there's also if that quits working there's a battery and when it's parked or whatever they it's like a Prius you know whatever the the gas engine will charge the batteries but this is all composite and they called it a personal helicopter this was back in three or four years ago when people weren't thinking like this anyway the cool thing about this is it's autonomous and while the plans were I mean this is I think this actually was a testing model here I think they got it to that stage but anyway this is was planned on being autonomous and it has a display in it you know a flat panel display and the map comes up and you just push a button where you want to go and it takes you there and I think the top speed on it's 75 miles an hour and there's some small manual controls you can override to fly it but the point is it's meant to be autonomous and they're gonna sell this for 200 grand and anyway what happened was Steve Burns made a deal with a company called Moog and he sold this off to them and they agreed to go into a joint venture with workhorse to get a field trial together a prototype testing so on and so forth for their workhorse drone which I'll go into next but what ended up with this is Moog bought this there's some kind of conglomerate it's in another video I have anyway as I understand it this is going to be they bought it as a for a military application and their their thought or their proposal is to use it to supply frontline combat troops in an autonomous mode so carry medical supplies ammunition food whatever and you know no pilots at risk and it's autonomous and anyway that's that's that's that's the beginning of the drone program they had another parallel drone program which is this horse fly program and they've been working on this I think since 2012 and here's the thing the C1000 is the truck I don't think this is a C1000 I think this is a regular UPS truck oh it's a hybrid okay so it's not a C1000 I think we all know what the C1000 looks like by now integrated with the C1000 electric delivery vehicle a truck is a jumping off point for the drone and then after the drone makes the delivery they pair me they meet up and the drone lands on the truck and phone text message sorry and the truck features a control center for the driver and auto landing horse fly roof with the charging capabilities so anyway I'll show you a video on this in a second anyway this this is integrated fully into the truck the truck battery charges the drone the drone launches up and takes off and then whoever is getting the delivery they got a phone okay and I don't know if you set this well anyway there's a phone app you get a text message or whatever you go to a link and you get a view on your phone from the camera that's in the drone and then it's telling you where it's going and everything and when it gets over your house you know you get your finger and you say I want you to put the package here in the backyard and the and you can watch the drone on your phone and it flies over that spawn and then when it gets over that spot in your backyard it stays like 20 feet off to ground it lowers down a winch cord and drops the package up goes up and takes off pretty cool huh I know I could use this at my building because I'll tell you what every time the packages are left outside the porch pirates steal everything I forgot like six six packages stole I would just I would love to get a text and say where do you want the package and I position it right into the courtyard and have this thing hover over the courtyard here and drop the packaging would be great no more stolen packages and I would like make that to default so would do it every time I mean this I think this is a great system anyway it's not limited the only thing that limits it they said is really high electrical wires like them big towers they have those metal towers that go up they look like the Eiffel Tower you know those type of transmission wires and they said really really big trees which I don't know what that means but there there aren't many restraints on it here is a video of the thing in action okay so the girls around the EPS truck she stops there's the package all right she puts it in the drone she enters the destination address the top opens up I'm sure you've all seen this right and the drone goes up and the drone takes off with the package and the drones fly in and the roof closed and this chicks gone she's making her next delivery meanwhile the drones just out flying around on its own autonomously doing a delivery now she's doing another delivery there's a drone all the drone found the spot and this is an early version they've they've been improvements on this but anyway the point is the drone found the spot right delivered the package now there's a drone no package it's flying autonomously again meanwhile she's been doing deliveries she stops the truck to do another delivery right here's the drone drone comes back automatically plops on the roof parks again there you go gets covered up gets the battery topped off they go on to the next delivery she can do this all day so you know it can double or triple the work of of one person cut delivery costs and the thing is it's mobile and it's it's on the van and you know who knows maybe they could put two of these things on a van or three of these things and you know boom have them take off and have a guy you know I mean I think it's a great system and it's kooky and when it first came out it's kooky and when I first saw it I said what is this this is nuts all these things roofs opening up it's like the doctor know or something right but anyway actually they got it all worked out they've been working on it for years they're doing the medical deliveries in this joint venture with this MOOG subsidiary in South Carolina and they're just on the on the edge of a FAA approval it's all FAA compliant so I mean this is this is gonna happen anyway here's the here's the drone what it looks like again there's I don't know how old this picture is there's different there's different versions of it but anyway it's mounted on top of their C1000 delivery van okay that's how they got the system worked out yeah it's autonomous it's all FAA compliant they had to do all this safety stuff if it fails it breaks apart and all this stuff anyway it's got a 45 mile per hour top speed it's quiet and again there's all these redundant safety things built into it you can look them up anyway you know the propelled blah blah blah whatever anyway and it can operate in a 25 mile per hour wind or rain or snow no problem and the cool thing is it has a winch that lowers the parcel 20 feet so it kind of stays out of earshot even though the customers it's serving it stays up there and it lowers the package and it takes off so it's cool it's quiet too it doesn't come down to the ground and set down and you know have the whirling you know meat meat cutters flying and everything you know stay back Johnny stay back from the propellers you know that kind of thing anyway so it's got a winch that drops the thing down so you never have to get close to the thing and it's quiet and like I said the driver the driver sets the delivery I don't know you know I'm sure they could just you know flash a QR code printed on the package to the to the drone or I'm sure they got it set up some slick way of they just throw the box in the thing and it takes off and again the customer you know I'm sure they get they say your package you know you get a text your package is coming today between four and five watch for a text whoa here's a text the trucks at your block blah blah blah then they say other drones taking off and click this link and then you then you go and you know there's a camera looking down at the bottom of the drone which is feeding video to your phone your phone app and then it gets over your location and you just put your finger down and make a dot where you want it to drop the package and it maneuvers itself into that position at the right altitude lowers the package down there you go Bob's your uncle I think it's pretty cool then it flies back to the truck even if the truck's off take them making another delivery so I would like to get packages like this especially with the rona going on no contact deliveries it's great plus like I say stay behind the security system here wonderful I think it's a great system now just a couple words about this C1000 van the Burns Steve Burns was head of the company when they came up with this but this is a hub wheel motor it's a hundred percent composite body and frame illuminates 4,000 pounds of chassis and body weight compared to an ice vehicle carries the same cargo volume and payload being 4,000 pounds lighter this weight advantage with its ultra low floor and the dry red a quote from a driver in a reddit and you know this is one of the reasons I bought I got into this security there was this driver and he goes he was like a UPS driver something he said in this ready goes I just bought I just bought I bought this stock at a dollar you know I bought you know I don't know how much you bought but anyway the point is he goes I drove one of these in a test or something anyway he said it's the nicest delivery van it's the most driver friendly delivery van that they have he was talking about the turning radius and the performance and all this but the main thing he said was this ultra low floor it's a small step it's like seven inches off the ground and that's what you get when you have these hub motors and the driver said with the other vans you constantly climb it up and jump and down and climb it up and jump and down you see all those guys are wearing back belts and everything I mean can you imagine doing that a couple hundred times a day you know jumping down two feet climbing up two feet this is seven inches it's like a normal step and then anyway the guy said he loved it so much that's why about this anyway the ergonomic and cost-saving benefits although the trissel beats more appealing so anyway between the ergonomics and we went through the cost savings just using an EV cuts the delivery cost from a dollar to 34 and then you throw a van on top of this thing and it it cuts the cost of four cents and then you got the lower floor right so the driver is going to be happier there's going to be less injuries and they're going to work better workflow and they're going to have a drone doing half their deliveries I mean this is going to be such a productivity booster I mean anyway and again down here composite body thousand cubic feet I don't know if I have another video you can look at it I think it's ten things you need to know about Bob Burns I'll put a link in the thing where they where guys shows us how big this truck is and anyway and the C650 version which is the 650 cubic feet which I believe is that red one at the beginning it's going to be the postal vehicle I don't know that but anyway the range will be a hundred miles that's what these guys travel a hundred miles so anyway that's just about the work horse C1000 van now let's get back to this Amazon drone delivery so anyway oh there's the workhorse this is an older version this is not the new one but it just shows you the way this is set up with the drone and everything so all right so anyway this is what they're doing let me try a different I'm sorry I'm trying on your system here all right so why would Amazon with this with this truck based zone drone it's the obvious solution right it's the best what does Elon say first principles I don't even know what that means first principles don't get arrested don't get any pregnant don't even pray it don't get don't get hurt that's my first principles anyway so why would Amazon do this would you would you want this thing what the with the Wizard of Oz monkeys flying around there even worse would you want this within 10 blocks of your house constantly seeing these drones flying in and out of here 24 hours a day flying back and forth and trucks company I mean they want to put they they want to put these within 15 miles or whatever the customers I mean they're gonna put them right in the middle of the name right in the right in the middle of towns and everything I mean a blimp you know you know the flying monkey haven I don't know what they're thinking I mean I don't know they're smart right what do I know anyway this I think I got patents on both of these so why would they do this though I mean it seems so obvious to me what the solution is is is the truck based zone drone so let's why why would they do this because work force has a patent they got a patent April 7 2018 on their drone truck based drone delivery they got a patent on the idea right here work force officials announced United States Patent Office issued a patent number nine billion for the horsefly truck launch drone package delivery system and UAV package delivery integrated with electric delivery trucks so this everybody's talking about the postal order which is massive but even without the postal order with this C1000 with the way this truck's designed and the ergonomics and the and the and the way the whole thing works and then integrating this drone into it and having it patented I think this even more than the postal regulation postal market is is why the stocks undervalued because you know what the Amazon say they're saying it's going to be forty three billion dollars what a market anyway that's my take anyway I'm not a financial advisor good luck in the market thanks for watching the video


Here's supernova 2018 GV, the Type 1A supernova in the spiral galaxy NGC 2525, 70 million light years away. Hubble began observing it in February 2018 after the supernova was first detected by amateur astronomer Kochi Itagaki a few weeks earlier in mid-January. This was one of the supernovae used as part of the program to precisely measure the expansion rate of the universe that we covered in the How Far Away Is It segment on the cosmos. In the time-lapse sequence spanning nearly a year, the supernova first appeared as a blazing star located on the galaxy's outer edge. It initially outshines the brightest stars in the galaxy before fading out of sight.


Let's talk about Iran so and I'm happy to take take questions about what's going on in Israel Hamas as well But let's talk about Iran quickly So a Couple of pretty I mean one is an expected story wants a pretty stunning story But anyway the first which is another tragedy another tragedy out of the Middle East another tragedy every Iran I don't know if you saw the story. There's actually video of this circulating on Twitter and elsewhere of a young girl I think she's 16. I think she was 16 who went on to the subway in Tehran and not wearing a hijab and never came out She was beaten She was beaten senseless on the subway. She was taken out You know in a on a stretcher Beaten by men by the morality police who caught up with her Much of this was caught or at least the the beginning and end of this will caught on video And and that video is being circulated from cameras in the in the subway into Iran Her name was Amita Garavand Amit are Amita a are M.I. T.A. Garavand and She is another victim of the Iranian morality quote quotes police And Anyway, she was in a coma Until yesterday where they decided to take her off the machines and she did die yesterday and the family was notified The family was not a lot of bury her My assumption is that they did not want the family to see the body They do not want to see the family to see the extent to which he was brutalized by the thugs in the so-called morality police and You have a regime that legitimizes such stuff that encourages it You have this the most ugly treatment of women possible on earth And yet we still see leftist intellectuals feminist leaders Supporting the Iranian regime supporting Hamas supporting the activity again It really unthinkable The horror the women are going through in Iran right now particularly young women particularly young women who would like to be free I mean there are many reasons to get rid of the Iranian regime and Suddenly the American government and the American military force should not get rid of the Iranian regime for the sake of these women But since we have an interest in getting rid of that regime anyway Why not? You know handed over to these women let them run things So it really horrific this girl beaten to death died in the call died in a coma And and and became another one of the women who have died for the cause of their own liberty their own freedom We will see whether her deaths results in the kind of demonstrations That we saw last year at the end of last year. I hope so I hope at some point this takes off and becomes a real revolution in Iran. It does really does seem like The West will not take care of you on Maybe maybe it'll take the women of Iran to take care of the Iranian regime and one of the horrors of this This is just such an inex inexplicable Inexplicable that is a word they mentioned of this is and this is not a joke because this this would be Probably out of the onion kind of a Ridiculous kind of a parody of the world in which we live, but this Thursday just as the young 16 year old You know just as Amida was lying Dead in the hospital beaten by the Iranian morality police this Thursday The Islamic regime in Iran actually is coming Thursday, so this is after it was announced that she was dead The Islamic regime in Iran will become the chair of the United Nations Human Rights Council social forum What do you say about that? Right given everything that we've seen and how they treat women over the last year By the way, the foreign minister of Iran was visiting the United Nations Isn't it stunning that the United States allows this monster onto American territory and allows them to visit the United Nations In New York City, but in spite of the fact that Iran has public executions with no fair trial The fact that homosexuality is A crime punishable by death The fact that the Iranian regime is a leading sponsor of terrorism the fact that they fund terror and destruction all over the Middle East The fact That they treat women the way that they do None of that matters to the United Nations None of that matters Iran is now the chair of the United Nations Human Rights Council social forum How can even be a member of the forum? Human rights human rights the concept means nothing zero zilch it is eradicated It is void if Iran is considered a member of a human rights anything It's a this is a travesty. I think he was God, what country was it that suggested that they might leave the United Nations? I see the woman's face, but I can't remember who it was Anyway, it's a travesty Every civilized country in the United Nations should boycott the United Nations should leave the United Nations The United States should have long time ago sent them packing Kicked them out of the New York City New York City is too good of a city even in its common state To host the United Nations pack them up send them to Caracas or to Tehran Or somewhere like that, but there is no reason no reason To sanction an organization that does this again, we're talking about it You know an Iranian regime that has killed women that is massacred protesters There's just no excuse