everybody. We are here for a demo workshop from by Maddie Griswold talking about spatial omics overlay, overlay of structurally proficient, profiled regions of interest on the GeoMix DSP tissue images. And so Maddie, if you would like to come on and share your screen. And again, for people on the chat, or if you have any questions, please put them in the chat. We'll take questions at the end unless you want to take them at the end or as they come. Maddie, do you care? Either way, it works for me. Okay. All right. All right. Go ahead. Hello, my name is Maddie Griswold. I'm a bioinformatic scientist at Nanostring Technologies. Nanostring is a spatial biology company. And so a quick overview on spatial transcriptomics if you aren't familiar. At the beginning of RNA sequencing, we typically had bulk RNA seek, which essentially means that you took your tissue, you put it in a blender, and you sequence that. So you could really see the homogeneous mixture of the tissue, but not much more past that. The next thing was single cell RNA seek, which most of you probably know. But in this case, you could pick out the fruits in the smoothie and get the genetic profiles of that. But you still didn't know where that happened in the tissue. And that's where spatial transcriptomics comes into play, where you can actually see the structure of the tissue here in this fruit tart, where the actual location of each fruit means something. And that's what we're trying to get to in spatial biology. So Nanostring is committed to open source tools. We are trying to foster a community of open source method development for Nanostring data. And we are doing this. We currently have a dedicated internal open source development team that I am a part of. And we have this team so that we can ensure high quality analysis for tools or for high quality analysis tools for all Nanostring users. And we're trying to standardize methods with the thought that since Nanostring scientists are also going to be using these methods, that it will be the best method to use by people downstream. So a quick overview of our ecosystem today. Our main package is Geomics Tools. And this is the S4 class that we use specifically for our DSP data. It's using a expression set framework right now. And this will get you from data loading through QC and normalization ready for you to do any analysis that you want downstream. We currently have over a thousand unique downloads and it grows every day. Our next package is Spatial Decon. This is a spatial cell typing deconvolution method for spatial data, but it was built using the DSP data. This also has over a thousand unique downloads. And we recently had a paper published earlier this year in nature communications describing the algorithm. But essentially we take each of the samples and we can get the cell type proportion and population in each of those samples, which is pretty cool. We also have a background modeling technique called GeoDiff that has a pre-print out right now. And the last tool that we have is Geomic Workflows. This is a vignette that goes through an end-to-end workflow on analysis. So from getting your out of the instrument to visualizing differentially expressed genes and a lot of other very cool analysis techniques. But even with all of these packages, we still are missing the image. So when you Google spatial biology and look at all the images, you're very the first thing that you notice are all of these beautiful images. Or at least that's what I notice when I look at these images. But when you look at the analysis, this here is a pre-print of a internal paper past this one image showing where the tissue is, where we were getting samples from. The analysis still sort of looks like an RNA-seq paper. So other than this one image down here. But the secret behind that image is that it was actually done in Illustrator or Powerpoint. So it takes a lot of effort to first make these little pie charts and then hand place them onto the image. But the community itself can see that there needs to be images in our analysis pipeline. Here in Surat, we're looking at a Visium data set and we have a very beautiful image there. And even yesterday, the team showing spatial feature experiments was showing that images are present on people's minds. But what happens when our data isn't spot based? Computationally, it's really easy to create a pattern of spots. But what happens if we don't have that data? At NanoString, our data can take any shape imaginable. These are five different ways that we can profile tissue. And it's really driven by what the tissue looks like. So geometric profiling essentially means that you can take circle samples, you could take squares, you could circle around tissue, like hand draw them, that sort of thing. Segmentation is a very cool profiling that you actually take this image and our instruments can take the yellow image and make that its own or the yellow part of the image and make that its own sample. And then the pink part of the image is a different sample. So we can take that out into biological compartments. And then this contour sampling technique is you take this pink section here and you want to go equal amounts away from the tissue of interest. So what happens on an infiltrate boundary? What happens if you get farther away from that or closer to it? That sort of thing. So with these types of profiles, you get very unique sample sizes and shapes. So these two data sets, on the left we have mouse E15 embryo and specifically zoomed in on the heart section and on the right you have mouse brain. We have very different looking samples. So looking at the heart, these are hand drawn regions of interest or ROIs that someone painstakingly went through and wanted to look at these structures specifically. And then here on mouse brain, this orange and yellow section are two different samples. So the yellow in this are neutrons and the other ones are the orange's neutrophil sections. So but these images come either directly off of the instrument or these ones specifically are from our Spatial Organ Atlas website. And they're using very specific software that is either hard to use, takes some time, is in a different language than most of the analysis is done in. So it's a little bit hard to actually do analysis or make a publication worthy image. So that's where Spatial Omics Overlay comes into play. We wanted something that was R-based which is where most of our customers are the language that most people are using. We wanted a fast solution and we wanted it to make beautiful figures that could work in any manuscript. So that's where Spatial Omics Overlay comes into play. Both of these images are on the same data set that I showed previously, but done in R are ggplot based and are very customizable. So here's that part part and here's the segmented R-wise. So excuse me, each of these are overlaid exactly onto the image, exactly where they're meant to be, and you can customize them to be whatever you want them to be. So an example of this is in that same pre-print paper, we wanted to highlight genes on tissue substructure and actually showcase these. So if we look at this first one, we're looking at tissue substructure which are highlighted or are colored like this. But if we look at EPCAM, which is an epithelial tissue marker, go back over here, look at the pink sections, you can see that the gene expression of EPCAM is only shown in epithelial cells. And we can go through that with all of the other tissue markers. So while you can show this in a box plot or any other plot that you can think of, it's much more striking when you can actually put it onto the image itself. So all of this is able to be done using the OME TIF file that comes straight off of our instrument. So this is our standardized file that comes from the OpenMicros V group. And in this OME TIF, it contains two files. It contains a pyramidal TIF, which is all of the images. It actually has multiple images that are at different resolutions. So if you don't need to be working with the largest resolution and want some faster rendering times, then you can work with a smaller image and it's the same data. And then the XML file looks something like this and it contains all of the information that you need for this. So it contains the mask coordinates, it contains the fluorescent colors and all of that sort of thing. So all of our data comes from this one file, which is super cool. So all of the data is directly from the instrument. There's no guessing on where it might be on the image, what this coordinate might be. So it's pretty cool. So we're going to move over into the demo now. Are there any questions before we move to the demo? So here we're loading in the overlay package and geomics tools, which is where our data is located. So the only files that you'll need for this package are the OME TIF off of a geomics instrument. Right now it only works on geomics, but with other parsing functions it should work with any other OME TIF out there. Then a lab worksheet that matches the samples in the OME TIF to the annotation file. This annotation file contains any type of annotation you want. So it can be count data, it can be what type of segment it is, where the tissue came from, all of that. So the first step is just to get your TIF file. This file is just a just a file path to your TIF. This is currently a function to download the mouse brain data from the spatial organ atlas, which is the data I showed beforehand, and it caches it so you only have to download it once. So now we're getting our lab worksheet and then we're reading in the spatial overlay object. Here it's extracting that XML and then it's going to be parsing it's both for the image data and then for the coordinate data, and then we'll be generating the coordinates from there. So while that's running, I will say if you have other, sorry to interrupt because I was just checking we didn't have, do you have links for where these things are if people want to kind of follow along? Yeah, do they want it in the chat? Yeah, if you put it in the chat, then I will get them a not quick but onto the website, but then I'll figure out a way to get them quickly to people in the room. So that first one is the vignette and then the second one is the package download. Is everybody on Slack and all? So while this is still generating the coordinates, if you have data that's not geomics data, then these are all of the functions that go into this wrapper. So you can make functions similar to these for your own OME tiff file. So now that we have the object, we can look at that object and it's a spatial overlay object. This only works on one slide at a time. So here's the name of your slide, how many overlays you have and the names of all of those for geomics data. We have to know what panel is used and then if any of your data is segmented. So there are, I'll show this a little bit later, but there are three different ways to plot your images and one of them is outline. This means that we are only taking the outline data, but if your data is segmented you cannot do outline. So this is what the object looks like. There's some other accessor functions, getting all of your samples, getting the slide name and getting some other metadata shown here. So right now we're going to start this because it takes a little bit of time. So right now we're going to plot the overlay data and we're doing it at a low resolution. So this will allow a little bit faster rendering times, but if we go back to this real quick, without adding the image all of the coordinate data is based on this largest resolution. So it takes a little bit of time without the image added because it's trying to plot all of the points that are available. So let's see. While that's thinking we can show it over here. So this is what that image looks like once it plots and this is again the low resolution image. So the edges aren't crisp, but if you do it high res you plot all of the points and it becomes a crisp image. So yes, we're still waiting on that. Like I said, it does take a little bit of time to run that, but we can see it on the knitted vignette. So the next thing we're going to do is add plotting factors because on this one we're just plotting the sample ID and that's not very informative to start with. So here we're reading in the lab worksheet which just a data frame of annotations and then we're going to read in a geomics set object which has all of our count data. Let's see it has more annotations like slide name and scan name and then all of our count data here. So from these annotations from the lab worksheet we're going to add segment type from the geomics set object. We're going to add the expression of calm one, gene calm one and then just to show that it can we're adding a vector called our ROI label. So plotting factors can come from data frames, matrices, geomics set objects and vectors right now and your plotting factor can be either a column name or a row name and it will automatically find that data. So if we look at the object again we can now see that these plotting factors have been added and if we look at them we can see the segment type has called ROI, the calm one, gene expression and then that arbitrary vector that we added. So I'm not going to plot this because we're still it's still going to take a while so I'm going to show it on here and here you can see that we're plotting by the calm one gene expression and all of these plots are ggplot based so you can customize them exactly like you would on any other ggplot figure and a scale bar is generated every single time that you plot the image if you want it. There's also you can do scale bar equals false and it won't calculate it and won't add it but here we're doing a scale bar that's a 30% of the width and we've colored it to green so it's fully customizable just like everything else in the image. If we go back to here we're going to be adding the image so we're going to choose resolution six in this dataset there are eight layers going back to this so actually it might be easier to see it on this one sorry for all of the flipping of slides. So in the OMETIF resolution one is the largest resolution and as you go down it gets lower in resolution and each of these images are half the size as the previous resolution so in our image if we can check the lowest resolution here and it will be resolution eight so for the next or for the continual continuation of this workshop we're going to use resolution six and then we're going to add the image onto the object so we take the object the tiff file and then whatever resolution you want. This addition of the OMETIF can happen at the very beginning of making the object so if we scroll up here as it's calculating the coordinates again um we go up to our read spatial overlay in this case we did image equals false just to show the what you can do without the image but you can set this to true and it'll do this same process that is that we're doing now. So it's recalculating the coordinates every time that you add an image it's going to recalculate the coordinates because the coordinates start off on resolution one and we need to scale it down to whatever resolution you choose so that it continues to match but once that is done we can plot another image this one we're going to plot by segment type we're going to put a scale bar in the top center of the image and it takes up 50 percent of it and we're going to color it a cyan color now that that's finished calculating the coordinates we can look at the image or the object again and we can see that an image is added the images themselves are magic pointers but in the show function we're showing where the image came from and then you can do any of the magic um functionality on that pointer but show image ends up um showing the image in your browser and then we can plot this like I said um this plotting is going to be faster now that it's at a lower resolution because there are fewer points on it there's that scale bar so that's the main functionality but there are a couple of um addition or things that you can do on top of that so one thing is to add visualization markers um these are um you know fluorescent images so it's nice to know what markers were used for that so the easiest way to do that is just add for legend equals true and it'll add a legend um on your image where all of the other legends are at um but sometimes it does get a little bit to read like this yellow one um but this is typically for more um playing around with the data and not really your publication ready data set or your publication figure and that's where Cal plot comes into play we're going to make up a spatial overlay plot and then we're going to add a fluorescent legend that looks like this so again you can't really see the the white and I apologize for that but um it has all of the markers in their colors and then you add the cow plots on top of the other image so this ends up taking a little bit of time to correctly place the box and know where to put it know how big to make it um but it does look a lot nicer when you're ready for a publication ready figure um any of you probably noticed that this brain um is upside down for what we typically um are used to so here we can flip the image um and it'll be saved into the object so next time we plot it we'll have everything flipped over the y-axis um but if you don't want it to be saved you can also just plot it um so we're going to flip over the x-axis on this one um so this data won't be saved but you can plot it in that way you can also crop the image so in these images there's a lot of black space around the tissue um that takes up a lot of space so here we can crop to the tissue it'll automatically find where the tissue is and put a buffer around it so here we're plotting that same cropped image um by ROI label which is the arbitrary vector that we put on the data and it's colored by um it's a brightest color scheme and then we can also crop by sample type or samples um so in this case we're going to choose all of the full ROIs um in our data set and we're going to crop it to include only the samples that we want so only these full ROI samples and so we're going to crop by that and the tissue goes over to where the see if I can get this in one view tissue goes over um to only show the full ROIs but we can also crop where we want any sample in this field of view it doesn't matter if it's the sample that you picked or not and we can do that as well and then show that so in this case once it plots we now have not only the full ROIs but the segmented ones as well and the last um bit that we have here is you can recolor the image um here we have to add the four channel image again and then um can I do that okay um this might take a little bit of time um because we're adding the image again but what here we can go back to this once we add the four channel image um this is the fluorescence data that comes out of it um and you can see the color code this color code is what determines what color the image um color the images so here we're going to change our fitzy dye is this one right here we're going to change this hex code to another arbitrary hex code and then we're going to change alexa 646 which is this one right here um we're going to change this color code to magenta and then you can change the coloring intensity as well that'll change here so after those changes you can see that they've been they've been done and the listed color has also been updated with that so then we recolor the image and this is the image that you get but the the color of the image should be the same color that comes off of the geomics machine if you've played around with the coloring on there it should be the same this is just just in case it's not the right color that you that you want so um and so that's the that's the package um right now um currently it's released on an internal website called geoscript hub um and there's a download tarball there and a vignette which are the links that I put in the chat um and we're hoping to get this released on bio conductor in the fall but in a couple future releases we're hoping to add graphing on top of images so using those x y coordinates being able to add graphs on top of that um we're going to make sure that this is compatible with our new cosmetics machine that's coming out this fall which is um at single cell resolution so this package should work all the way down to single cell resolution we want to be able to extract data um to put into machine learning applications and after yesterday's spatial feature experiment we um we think it might be nice to have a coercion of our um our object into that um for other people to use okay do we have any questions people in the room thanks for the talk uh have you tried overlaying segmentation results onto the image for example for the cosmetics platform that you are going to release haven't yet um all of the segmentation this essentially is the segmentation results um for our data at least on geomics so we haven't gotten to um the cosmetics data yet yeah because with a cell segmentation there are a lot there are going to be lots of polygons so it's going to be very slow so it could be very different yeah um the cosmetics data will most likely use the um I don't actually have a good picture of this the outline function um and so it just does the outline points but um I at that point it might be nice to optimize it and maybe go to like an sf package or something along those lines um to try and speed up times somebody else here I haven't seen anything online but we were able to get your links up there are now on the website of the bio bio c 2022 website for people to look at um we have some extra time I don't know if you had any live coding last minute stuff you wanted to do I don't think so unless anyone wanted to see um a specific thing off of this data hi I was going to ask does the package do any like quantitative analysis where you're comparing expression levels it doesn't um it just puts that um that data on an image for you okay great oh hey maddie maybe if you can pull up geomics workflows so that they can actually see that quantitative part it's in a separate so these are in a separate package and maddie's package is supposed to build on top of that quantitative analysis so you do your quantitative analysis um these packages have been released for a while and then you move on to maddie's package to to make your publication ready figures yep that is true so this is the geomics workflows that I was uh that I showed at the beginning or at least a screenshot of it this is a step-by-step walkthrough from uh beginning to end and so it goes through qc um and all of the qc types that we do normalization and then let's say that you want to go through your differential expression genes and you pick out the the gene that you want to show so maybe in this case you want to show off clic5 and you can show it between the two um two in this case tubules versus glomeruli and kidney um and show the expression over the top of your image okay we did get a few questions on the chat all right in the background um so one asked is this workshop on orchestra which I believe is no correct um I don't think so I didn't add anything to it so no but um the workshop will be the same as the links that I put in the chat okay yeah so they will just have to run it on their own I guess that leads to this doesn't need any everybody can run this on their laptop they don't need anything special fancy or large uh memory memory yeah it will depend on what image resolution you want to use um and what your instrument or your machine can handle um I have been running on a server and can't do bias resolution um but maybe your instrument or your machine will end up being at a lower resolution okay and then uh there's another question on and I'll read it out so everybody here can see it is but from Dana King apologies if this is a naive naive question question can you explain the magic pointer in relation to loading viewing the image yeah so the magic pointer is a um it's a package called magic that's um spelled like magic with a k or a ck at the end and so if we go into oops mu brain and look at the image um so it's I don't know the back end of their package but this is just an image pointer to um I believe your image file so it does it um it doesn't take up as much memory as uh actually reading in your image um and Ryan said in the chat that yeah it's the image magic bindings for r so yeah right now if you point or like if you call the image the pointer it'll just pull up the image there are no last questions last questions okay everyone join me again and thank you Maddie


Hi everyone, Joe for jazby'scasebreaks.com with 2022 Panini select football six box half case picker team six The second half of that case that I popped open a few hours ago Big thanks to this group right here for making it happen. Appreciate it If you got mosaic 15 next to your name That means you won that team with that filler pack that we did in Victor You ended up with that last spot mojo before we pulled the remaining teams See what we got Jake burger tying up the game against my Dodgers with a second homer of the night Games tied for four Silver lining Jake burger is on my fantasy team. So I guess that's good. I do need the offense on my fantasy team last year we we asked You know last season I remember talking about hey, you know joking around We'll put up doing baseball breaks and pulling Jake burgers like a Jake burger Does Jake burger have his own burger at the White Sox stadium and someone chimed in in the chat. Nah, he's not he's not good enough I wonder after this season. Maybe Jake burger could get a signature burger Yeah, I got torgless on my fantasy. I think he had a dinger today, too. I mean, he's got it. I think he's Feel like he's on the cusp of heating up You know the former number one overall picnic on the cusp of heating up. He just needs He just needs to kind of keep that going for like A week two weeks a month Here's Trevor Lawrence piece of his jersey That's gonna go to Matthew Yeah, I guess Spencer always feels like he's a year away from From being a year away There's Trey Lance to 149 for the Niners. That'll be for Sean Maddock, but hey, he's a former number on overall pick This is what his only second third year in the league So I feel like he gets a decent leash John with the Falcons John's and Taylor had some commentary about the state of Running backs and how they're being compensated It's a rough time for running backs the numbers show that I say a spill are going into Johnny and the Chargers the numbers showing that running back effectiveness in their career not very Not as long as some of their other As long as some of their peers There's Josh Allen tie-dye nice to 25 And that's a nice field level right there. It's awesome It's Carlos in the Buffalo Bills Yeah, not good for the hobby. I mean, I feel like people want to collect running backs There's sauce Gardner to the 75 and Bobby Wagner Jersey and Otto nice Seahawks that'll be for Adam Koperman 34 out of 75 Patrick with the Jets gets the sauce 4 out of 35 John Mechie Rookie Otto for the Texans That'll be for Wayne There's one Dale Robinson to 199 and a Christian McCaffrey sweet level One Dale Robinson is going to Eric and the Giants That is what level is this premiere level. Yeah, we need more Derek Henry's I agree, but That's just not with the way You know, I think I'm a big believer in like sports running in in in cycles, you know, like One trend will come in and go out like, you know, there could be a Time where we see dominant You know big men, you know, like Shaquille O'Neal type big men in the league You know where there used to be a lot more of those back then playing a certain style You know, we're seeing base running coming back in baseball, you know, so maybe we'll see some kind of change where We might see the return of Of the running back. I think guys like Josh Jacobs and and Seyquan Barkley and Dalton Cook, they're gonna have to realize that They're gonna have to realize just that the running back market is tough I don't blame GM's and owners not wanting to pay running backs look at look at Ezekiel Elliott to Marco Murray looking You can go down the whole list You know There's Here comes McBride Jersey and autograph for the Cardinals Peter with the Redbirds 185 out of 199 Eric wishes exceptional corners were valued more Corners are tough too because You know, unless you're just racking up interception numbers There's Aaron Rodgers Green Bay addition to 149. Let's go to David sometimes corners won't get those those Delicious interceptions because no one's throwing their way Here's Terry McLaurin three-color pass for the commanders Joshua With that one nice tie-dye parallel 20 out of 25 But somehow because they don't exist well sometimes they they don't get those numbers because they're not throwing that direction although Tyler's pointing out listen, maybe Because of their quarterback they have to play Derek Henry that way but Derek Henry has been fairly durable though Here's Cadarius Tony to 49 sweet level and a Desmond Ritter silver Nice John Jackson with the dirty birds Premier level on that one. Yeah, I think I like the Raiders draft in general, you know, it's been it's been a minute or two since now, I mean, I don't know like What they do with that, you know, maybe on-field coaching Could be something to be a little desired from Josh McDonald's and the crew but Draft-wise, I think there was a solid draft Raiders need depth. I mean They've had so many terrible drafts are so long that they just don't have the depth that they need to be ability to rotate players around With nine out of ten field level Romeo Dobbs It's for David H. I think that's the field level is the shorter printed of all the four or five different levels now They should trim down the levels a little bit but Levels feel like they're getting a little bloated They do look sharp cam Taylor Britt Rookie red to 149 for the bangles. That's gonna be for Jeff another box You're wondering why I tear tear tear it open like that Sometimes we there's like select packs that like fall sideways and so they can get hidden and then there was one year This is many years ago There's one year where I slid my hand in too quickly to get the packs out and I sliced my thumb Right against the sharp cardboard edge You should stop doing that too Too many levels. Yeah. Well, yeah one neck locker room level it Before you know previously it was just like field level Premier level and concourse level or something like that. I think that was it Dodgers pitching is a problem ladies and gentlemen Especially the relief pitching Not the Dodgers kind of figured it out in the last year or two Become another weak point Starting pitching a problem, but that's they're gonna fix that too losing Dustin May was not good Some injuries early in the season for some guys Here is Dak Prescott sparks relic 87 out of 99 That's gonna be for Ed P and Dallas There's Lou Godke Or Gideki 4 out of 10. That's for Victor and the buccaneers and a Kenyon green Premier level gold 6 out of 10 that velocity pattern in the background looks sharp That will be for Wayne and the Texans Yeah, Caleb Ferguson who's been pretty steady Getting hitting around a little bit part part of it's just part of it's just the ebbs and flow of a little regular season, but It happens Pierre Strong, Jr. 66 out of 199 rookie jersey and autograph for Ron and the Pat riots Jonathan Owens to 149 for the Texans There's Reggie Gilliam to 99 What's smart? Oh, it's marthly because they know people collect sets and rainbows so it gets people to buy I agree but at some point if there's just so many levels that It becomes almost impossible ever complete the set then maybe they're just like, yeah Not worth it I'll just wait until someone else opens and I'll just buy it on eBay or something like that Jonathan Taylor to 149 All right halfway through this break Kind of like the smart tactic of showing people losing games on apps and ads I guess people would people say can't be that hard and download the game I think that's a little different example. I don't know if that's quite applies to this Mr. X, but I have seen the video game app thing though. Sometimes I'll see people actually make full-on videos of like of like someone being like Who they show I'm actually downloading the game and playing the game Yeah, the people who claim that we're in junk wax era 2.0, I mean That's also not true As much as they want it to be so they can be angry about the hobby It's just Unfortunately not unfortunately, but just just the way it's gone It's just expensive now This isn't hardly junk wax that $700 a box 750 a box hardly It's a lot more stuff now. It doesn't mean more doesn't mean junk. There's kind of somewhere in between You know of high-end stuff. There's still a lot of low-end stuff that comes out I'm sure the same people, you know if we roll it back to like I don't know if we roll it back to Oh Drake London if we roll it back to Drake London out of five sure one out of five Drake London London London Going to John Jackson. I'm sorry miss Jackson. Oh, I am for real With the dirty birds Drake London out of five and a train whistle All aboard the Big Head Express It's sharp and there's Breeze Hall out of 25. No, he bought he won that team in the filler As well even better Breeze Hall tie-dye to 25 Patrick Yeah, that happens all the time Rex. There's two out of 75 and Jeff Wilson Jersey and autograph tie-dye Even happens here it's still here on group breaks You'd be surprised at how many people love the product. They happen to hit in But but hate the product that they don't hit in they're always like, oh You know, not worse the cost of the box. That's And then I try to explain to them. Hey, you know because it's most likely they're like, no You're just a you're just you're just D riding for Panini and You know Blah blah blah blah, but what are you gonna do once once once people are convinced of something You know, they they they build the narrative in their head They would then they want to stubbornly dig in Nice rookie Otto Chris Olave Saints Jackson with the Saints not John Jackson, but Jackson W. I'm not gonna attempt his last name I feel like I'm gonna Jackson you're also gonna get this Trevor Penning as well. I'm gonna I'm gonna mess it up.  Hopefully I didn't butcher that. I'm gonna go Wilhite. There's Louis sign sin sine kind That I don't know that's gonna be for Douglas and the Vikings Do I know have to know what the biggest hit pulled by a customer in the shop? That I don't know no, I don't know Here's nice Devante Adams Sparks 34 s75 Ask me tomorrow Rex Before we before the shop closes then I can ask Evan He would know two boxes to go on Instagram. We do I Feel like a lot of people buy boxes and go home They don't they don't rip them here at the shop not as often anyway. I feel bad for Devante Adams. I Mean I yeah, I well I don't feel bad in the fact that He's getting paid very handsomely to to play the game of football, but I do feel bad in a personal sense you know in that Get him to Green Bay. We hook him up with his buddy Derek Carr and Then I Then Derek Carr gets unceremoniously benched and is away from the team and then off he goes like that was Maybe not the right way to do that Sort of weird They didn't get to say goodbye to the fans or he you know That's a weird situation raiders are weird We got Lamar Jackson. I'm sorry miss Jackson. Oh 36 out of 75 Lamar going to Michael and the Ravens I'm glad that he went back to the No, I went back, but I'm glad they just got that deal done You know, I think in the hobby. I was like seeing You know players stay in there. They're you know, they're they're uniform Their entire career. I know that doesn't happen very often, but kind of sign at least at least One or two more deals with the with the team before you go elsewhere. There's a son of Haskins to 199 That's true Tyler True words, I've rarely been spoken. Here's trailing Berks rookie Jersey and autograph for the titans EA With the Tennessee Titans You're getting a new stadium I'm pretty sure 23 out of 99 and I'm pretty sure they somehow hoodwinked the city and Got them to pay for Got that got the people to pay for most of it. I don't know the owners make a lot of money Like fund the stadium yourself here's Trevor Penning to 199 Why don't why don't owners go into debt? Building a stadium. Mr. Vonte Williams 249. Why stick it to the people? Oh, there you go. Yeah, Aaron Visited the shop got a box of 2019 contenders hit a Daniel Jones cracked ice autograph from the store sold it for 3k I remember that as Brian Robin to 149 Joshua with the commanders Justin Fields Go go. Oh Come on, Mookie 35 I thought he was lifting more weights in the offseason That that could if that was just one more day of push-ups That ball would have been gone. Yeah, it's the Marine layer Yeah, whatever happened to Michael Carter and Javante Williams. I Mean, I think that's just kind of What we were talking about a little bit, right? You know the the running backs just kind of hit hit or miss I haven't seen Freddie fooled like that on a pitch in a little bit.  Well, you mean that's third. Wait, when is that parade? I thought that was Thursday or no, maybe the Nuggets parade is Thursday Yeah, then Javante Williams did his ACL Which was on for I think during a Raiders game. Maybe Broncos Raiders game like non-contact So we'll see how he returns from that Devon Bush and a Cedric Wilson, Jr. 62 out of 75 For the Dolphins, that's Jackson W with the Dolphins Oh, it's on Saturday They're closing the strip from MGM to Bellagio That's a good chunk of the strip There's Evan O'Neill to 99 Did I say O'Neill Evan Neill? That's for the Giants. That's for Eric and we have Tyler Algear one out of 25 tie-dye Rookie autograph for John Jackson and the Falcons JJ with that one I was putting the card in the top loader and then it's stuck Anyway, it's protected. It's safe. We got orange Jamar Chase to 49 a little color match there for Jeff and the Bengals But Eric's like man Cedric Wilson should not have any Dolphins card Nothing for us. And we got Travis Etienne, Jr. Sparks relic Aaron, are you are you a Golden Knights fan? 30 out of 99 Tyler, do I have a favorite box I like to open? I do The fate my favorite box to open is select football. In fact Tyler There's more select football on jaskiescasebreaks.com right now. Check it out There's Evan Neal blue and Phil Sims Jersey and autograph ABC Tyler always be closing all ABC always be closing Eric Here's an old-school giant for you Phil Sims Jersey not a 182 out of 199 007 out of 199 Evan Neal and let's see what we're gonna end up with here is Jerry Judy We've got a tricolor Jonathan Owens for the Texans Wayne To 149 and we got a nice tie-dye Devonte Adam snapshots Alex really this is out of you think they called that 18 out of 25 as a Raiders fan growing up Adams idolized Tim Brown Now as a member of the team the Pro Bowl why does put his name ahead of the Hall of Famer? After breaking the team record for receiving yards in 2022 means a lot obviously Tim and I speak often and means a lot Got to call that right All right gang. There you go That was 2022 select football six box half case pick your team six a brand new release just dropped today We got a wax party invite to give away then we'll do a quick little recap So let's see who wins that wax party spot. Let's flip back To the list here one being given away Let's gather everybody's names one through 32 and let's roll it and randomize it five and a one six times Name on top after six fingers cross good luck one two three four five and six So there'll be a lot of sad faces here saddest faces spot two Very close Johnny appreciate you trying though two through thirty two But happy times after six four Steve Herrick Steve, I don't think I called your number very often in this break But with a spot that you won in the filler You get a wax party invite check out the the prize list on jaspys case breaks.com. It's a really nice stuff I'm Joe for jaspys case breaks.com. Thanks everyone for getting to select six Lot of nice stuff here a lot of nice color. I always always like to select design Some gold Chris a lot of a the tide eyes that out of five Drake London More gold a lot of gold in this. I like this set some good stuff here Look at that Great photography a lot of fun a lot of good stuff. Thanks everybody I'm Joe for jaspys case breaks.com and I'll see you next time for the next break. Bye. Bye


The drop last season, and today they present a keen slate at Crawley Town on what the day they pay host to Bradford City. One of us, Scott Lindsey, has made a raft of new signings this summer. There must be half of those joining from non-league sides. Lindsey, looks like he's got a right fight to his hands ahead of him. Los Angeles has got half of you on at Kala. We've no doubt been a lingering memory of them to the Bradford going into the new elite two campaign. They will be desperate today to start well. Well, he's just short in the Biles Munchies' side last season. That's got an opening weekend. The travelling numbers followed themselves to get off the mark today. He's looking for Jim and Rupert to stretch his legs, cutting in on the left-hand side, twisting, weaving. And shooting straight at Crawley, had I. The 18-yard books are a breakthrough here for the hosts. The conceptives and the Indians have not finished above 12th Crawley Town. Is that a free kick it is, according to the referee, Thomas Parsons, and he's going to go for his pocket and pull out a yellow card as well. Little harsh on Kelly on the hand of Bradford. Backline. He's got plenty of the ball. He's a deputy. Little right over the ball. 25. Cross yards from goal. It's a centre. It's right past the goalkeeper and into the back of the net. A debut goal. A dream start for Wright and Crawley Town. Whip and dip are plenty. Beating the wall and beating Harry Lewis. Beating the last seven lead games versus Bradford. Desperate to maintain that round for Crawley Town. Excellent home as well. Back in the last season, I'm beating in that last eight-point lead game to finish last season under Scott Lindsey. Colts and Sines thus far an opening day. Now Darcy to his right and left. Jordan is gauding into the box. Open up nicely for the flying. What a great wing-back position this afternoon. Darcy in the 10. Hit the 10 on his back. Fine Gordon backing on his left. But just stunned and stuttered. He invites the block behind. He will be delighted with the start of his side. Shocking into the box. Blocked away. The Brentford low knee. In half there. And don't tell for the poorly captain. And Dan and your go-k. You'll go into the book of Mr Parsons. The first this afternoon. Drifting, trying to impact the game. Ready for the opportunity. Just couldn't quite sell in the field. Telford followed in. In front of the bar by Sarula. Flooding red shirts forward in every opportunity, the hosts. Send it for Nick Sarula. Just quite keep his effort down. Half would have been created an awful lot thus far. Mark Drew's aside. Andy Cook into the game. 31 goals of course to his name last season. Walker has looked more of a threat though. Here's Jenny Walker, what a small twisty. Inside the 18-yard box. And a good save by the legs of Adai. Cute angle for Walker. A stab at Goldwoods. But Corey Adai stayed big. Stood up. With his shins forced it away. Felt nicely on the rebound as well. Towards half time. Here's Walker. Wrong side of the forward midfield. He's got right shots up with him. Holden is Richie Smallwood. Smallwood onto his right foot. Still going Smallwood. Creates a yard for himself. Adai drops comfortably to his right-hand side to save it. Penelope from Bradford. Some of the business competitors. The 17 new faces we saw at Bradford last year. Opportunity now. Orsi breaking away. Coming back to the midfield. He's into the book. And it could be a red card. On opening day for Dan. Before half time in early shower. For the midfielder. Not the debut he would have wanted. But his new club this afternoon. Catching Orsi. Bradford down to 10 there. Half the game still to go. The better of it. Downing that. In this first period. In West Sussex. Pulling the strings. Fizzing the ball. Orsi into Darcy. Rattles the woodwork. Again Crawley. Moving through the gears. Telford Orsi. Darcy. Woodwork smashed. A season. The pundas expect to fight ahead for Crawley this season. 45 minutes. And they're doing well. How will Bradford respond though? Got to improve surely. Mark Jews will fill that with his side. Kicked in towards the box. Into the five post. He started the move. With the penetrating run. Bradford Orsi. Another Bradford debutants. In the starting 11. The dealer just kicked Bradford's foot around it. In the end forced it wide. Attuned to again for Gordon. Orsi with the drive. Locked away. Into Lowloss. Shot Lowloss. Good save. Harry Lewis keeping right now. Bradford in this game. And then he slammed over the bar. Gladly Lowloss off the bench. Into the game. Lovely effort by Kelly Gordon. Lowloss. His marker strained it. Lewis had to give his best across to his right. And we'll see. Slightly rushing it. Slapping it into the side netting. Crawley will have the opportunities to wrap this one up on opening day. In lead two. Bradford. Toward an opening day lead goal since August 2018. Right wing continue for a little longer. For another year. Another season. The doers. He fouled in the edge of the box. Not according to the referee. Patricio with White Shucks. Can he afford in towards? Right hand. Then called upon. Has been there for Crawley down this afternoon. Hopped in towards the front post. Right hand. Struggling to get up and open. Create real power. It looked like it was drifting right in the end. But I didn't know that. Attuned as he left. He thought for Bradford to pump it forward. To get the ball forward before they run out of time. Scott Lindsay checks his watch. And punches the air with delight. Bradford for short on opening day. Here you go, Kate. Sends off before half-time for Bradford. A key moment in the afternoon's match. We're all right. What is Dave you goal? The match winner. This afternoon look at Scott Lindsay. Delighted. Bradford creates it. Can he push the hose? Lindsay delighted on opening weekend full-time. Crawley Tower 1. Bradford City near.


I'm gonna get you guys situated. I thought I was going live before pups woke up from a little tea-tiny nap, but turns out, right when I hit the live button, they woke up. And I'm trying to time this live in between fire alarm. One day it's a storm, the next day it's fire alarm. It's always something, but that's fine. So, hope we're all having an awesome day. What's up, Rachel? We are, we've been busy, trying to get orders out, trying to get prepared for class tomorrow here in Dallas. P.S., if you're in Dallas and you want to take class, I have a couple of seats open for tomorrow. And today I'm going to try to do a terrazzo, a terrazzo, but my way. And what my way means is it's gonna be glittery or shiny. I don't know, it's elegant, gaudy, I think, because I feel like that's my overall aesthetic. I'm looking for a spray adhesive. I know we have some, but beyond that, I'm not sure where it is. If you guys don't know what terrazzo is, I'm not even sure if I'm saying it right, but it is usually found in like, it's not cement, but it's like a similar, I should have done some homeworks so I could tell you exactly what it was, but live and learn. I have no idea what terrazzo is actually made of unless terrazzo itself is actually its own whatever. Anyways, I can't find my super 77 spray adhesive, so I'm just gonna use this photo mount. Actually, I'm gonna pull up what terrazzo is, gudy. So this is terrazzo, it's white with kind of layers of flecks in it. So I was thinking, I bet I could do that with resin even more, I was thinking, but I could do that with resin and make it my way and make it super awesome. What's up, Sue, Rhonda, Nancy, Cindy, Christina, Keri, TG and Gudy and Tim, I hope we've all had an awesome day. I'm not even sure what day it is. Judy also keep me posted. Anyways, I'm using photo mount, I'm just gonna spray it over, oh, oh, so it did that. So I didn't clean off the nozzle because I'm a professional.  I just want a light dusting. And I'm doing this so I can stick some of my glitter on the underside, the base, the bottom of my terrazzo because my plan is to use milky colored resin to make the terrazzo look. And then I'm going to do another one with interference. So it's gonna be an opal terrazzo with glitter. Anyways, that's my plan. And I'm gonna use this confetti collection from color obsession series number one. Collection number two. To me, this just screams of a very festive upbeat cheerful circus. And I need an upbeat cheerful circus. Anyone else? Just me? Cool, cool, cool, cool. So once my, this is tacky, you can hear it. Let me put my mic down there. I'm going to just sprinkle some of my this over it. Oh my goodness. Does it make anyone else want a fun, fettie cupcake? I know that's not just me. Know it. I want these to lay flat. So I'm kind of just squishing them down. I'm not putting too much on the base because I'm undecided on if I'm gonna do any clear or not. This could be a finish all on it's own. Courtesy of Rhonda. Forgot what I was saying. Oh yeah, I don't know if I'm gonna do clear. So I want some on the base, but I also don't know how much that's gonna show through.  So I've sprayed the other one. And I'll take just a second to do what I need it to do. For some reason, all I'm seeing is I couldn't see all the messages from you guys. Okay, so, New South Wales. I think we're gonna be coming to Australia soon. Courtesy of just resin. If you want us to come teach in your city, shoot them an email and let them know you want ATD in whatever city you're in. Also, I went ahead and mixed up my resin. So you guys don't have to wait on that. Knock off any excess. Make sure to use the bad boy grilling flat. I'm not gonna rub my hand circular over it right now because I don't know what it's gonna do and I don't wanna flip any sticky glitter over. I have a lot of glitter that, if this looks right, would also look good. But I'm going with this circus collection which I think that's what she should name it because that's what it looks like to me. Oh yeah, I'm gonna do this. It's not pulling any off. I'm just gonna make sure all you bad boys are laying flat. And we're gonna start mixing resin. So, set that on slide. Tiny town called Tumut. I know for a fact, I did not say that right. Hammett, Tumut.  All that ruckus. Anyways, this is what that glitter looks like. Super, super reflective. Also, you guys basically bought it while you did. You bought me out of all my little glitter yesterday. Thank you so much for that. And I still have some color passion, similar colors if you're still in the market. So, I went ahead and mixed my resin. I'm using Stone Coat Art Coat today because I'm using a lot of white. The majority is gonna need to be a milky white color. But I also need a little bit of resin for my interference blue, interference red, interference green, and interference gold. I'm gonna be reviewing some new interference scissors from Resinart soon, but she hasn't sent them to me, but I do have Judy's set, so I could review that if y'all were interested. Tumut? Yeah. I would have got that wrong. So, I'm gonna go ahead and mix all the colors so we can just roll from one of these bad boys to the next. So, first one I'm mixing up is Red Change Sparkle from Color Passion. It is kind of a diamond dust size flake that has interference gold in it. It also reminds me a lot of the Blingit Sparkle colors. This one would be Blingit Sparkle Red.  You'll find that in any micro you use, the bigger the particle size, the more glitter it will be, and the smaller the particle size, the more satiny it will be. So, for example, this one is Blue Interference. You can see the particle size already just in the jar is smaller. And once I get it in the this, that's probably too much, actually. Because the more powder you put in, the more kind of cloudy it's gonna look, and I don't want it to be too much pigment. Shocker, I'm doing easy pigments, but it's okay if it's a little bit milky because I'm gonna make my whites a milky color too. But you can see that this one is way less sparkly, but way more satiny. Both are in interference, but the look is a bit different. I'm not gonna use this one. I'm gonna use my other one. So I'm gonna use a green interference as well. I need to pull a gold and a red out of my other stash. These all so far have been from color passion, but I have interference in sparkle micas from a few different brands in the shop. What's up, Deborah? So most of the time I definitely pronounce things wrong. So if I ever pronounce something wrong that you guys say, apologies in advance, very literate, but when I'm looking out of the side of my eye to read something, sometimes it doesn't do right. I'm gonna put a little bit of interference red into my red sparkle because it's not solid enough of a color and this red interference that I'm mixing in is from resin art or color art is gonna be awesome. There we go. So now it has the sparkle and the satiny interference color all at the same time. Now I'm gonna mix up the gold interference. This one is also by resin art. Usually I would mix in a purple interference as well, but I don't have any more. On order. I expect to get my color obsession order this coming week, speaking of, that should be the next color to come in. Okay, now I need an opaque white and I'm leaving a little bit of resin in my cup for mishaps, random necessities, needing more of a color. All right, so first one I'm mixing up is my opaque and I'm gonna get, I mean, arguably a lot of resin paste to put in here. I'm using color passions, top cell whites. And I want this little cup of white to be opaque and the difference in opaque and translucent and transparent is that you won't be able to see through it. I want it to be full, opaque. It's a little bit translucent. It's not transparent because you can only just barely see the blue of the star stick shining through there. So I'm gonna put just tad more in and also mix up my translucent white by putting about the same amount of paste in but into this bigger cup. So I want it to look like non-dairy or skim milk, that like cloudy look. Thank you Tim for reminding people to hit the thumbs up. I'm really trying to grow the channel this year. If you can leave me your thumbs, leave a comment, tell me where you're from, which one of these finishes you like better, it will super help. And I somehow still made this opaque. I'm gonna put more, in fact, I'm gonna just pour all this in here. These colors are so pigmented that it's hard to make an opaque pigment translucent or transparent. Always air on the side of caution. A little bit, you can just add more if you need to. That's more like it. You can see the blue of the star stick a lot. Okay. Buck, how are you doing? You haven't art in a while cause life? Well, we miss you and we love you and we hope everything's okay. Shoot me a message if you need anything. P.S. The other day when I asked about shy, she popped in and left a comment on one of the videos. I'm glad she's doing well. Debbie says I just ordered the greenest glitter last night. Can't wait to play with it. It's super tempted to buy more.  Well, maybe sometime soon you can come over to our shop. I'm teaching a class tomorrow, by the way, if anyone's in town and wants to be part of my class. Okay. I'm teaching resin, ocean and geodes tomorrow. Here in Dallas, downtown. So I'm mixing some of my glitter directly into my milky white. My goodness, it literally looks like cake batter. Will you stop tempting me to eat cake? Okay. So the first one is gonna be flat out terrazzo and then the other one is gonna be opal terrazzo. I'm just gonna, I'm just gonna pour this out, right? Yeah, right. Now I'm second guessing myself, like should I have breaks where you can see? That's fine. I'm gonna stick to the plan and be really excited about the fact that I did not waste too much glitter on the base of this since I'm covering it all anyways. And I'm gonna let this set up a bit and then I'm gonna sprinkle some on top. There's not really a whole bunch of, nope. Skill, I guess there's not much skill involved in resin terrazzo. You just have to layer opaque and translucent colors with whatever your color filler is. This just makes me want to cake so bad, that's fine. Okay, here's gonna be the tough one. I'm gonna try to get you guys a better vantage point because it seems like, well, it still has a shadow. I don't know what to do to fix that. It's fine, it's fine. So I'm gonna start this one with some glitter in our interferences, just a bit, make our opal terrazzo. And I'm gonna start with these translucent because I'm in with the interferences because they're important for my overall opal feel that I wanna go for. Trust the process here, people. Now this, goodness, it just looks like cake batter to me now and I can't unsee it. Rumble, what's that? Oh, it is St. Patty's Day. I should have done something just solid green. Oh well, live and learn. I'm gonna try to swipe my interference colors over the milky white instead of the other way around so that that milky white doesn't conceal any of the interference that we got going on. I think I'm really gonna like this. Okay, now I have my opaque white and I'm going to give it the fault lines that I super love doing. Give it a moment to sink a bit. I think I'm gonna put fault lines in the other one too. Super love that glitter for sure, for sure, for sure. There's so many glitters that we carry that I have never even tried out just because there are so many of them. It doesn't give me a lot of time to try them all out, unfortunately, that's okay. So can you guys see the interference on this? Nope, nope, light off, even worse. Maybe if I turn this other light on because it just doesn't look anything like what it does in person. It's a bit better. You definitely see the texture of the glitter. This for sure will need to be blood coated, blood coated. Glad you're going through them so you don't have to. I see what you did there. What's up, Julie? The only problem with the fault lines that I love to do and having something so textured is that when you tilt them, it kind of gets wonky like that. It interrupts my lines. My goodness, I'm gonna bring you guys over here to see what this looks like since I'm done. So do you need more epoxy to cover them? So no matter how much epoxy I put on at this point, they're still going to do what they wanna do. And putting more epoxy on it now would just split the resin and interrupt the flow of the piece overall. So essentially, we're just going to wait. I don't know if you guys saw my video earlier, but the fire alarms have been going off in my building all day. I'm trying to hurry and do this before they go off again because I know they're going to.  All right, now that I have the base done, I'm gonna add a few more flecks on the top of this glitter and get my chunky hand in there. I might actually decorate this like a cake. Is that weird? That's probably weird. I need to switch out and top this other one. I think if I had a kid that really was into this circus, this would be a such a rad countertop for like a bathroom or a desktop. Sorry if that crunchy sound was loud. I think I wanna put like this may, but we can't even see what I'm doing. I may completely be about to wreck this piece, but I kind of wanna just fill up this, not maybe fill up, but put more in so that there's kind of a loud vein of color. Okay, super like it now. This is very not normal for what I do on the channel other than the fact that I do the test so you don't have to. All right, if Hershey wants me to do some countertops for him, what's up? I'm here for it. I think I'm gonna UTC matte these as well because you don't understand why you can't see the interference as much as you can in person. Maybe if I get closer. You can see the green and the red and the blue, gold streak. Do they make you lose the building every time it goes off? They don't, but I kind of never know when an alarm is real and whatnot. So it's difficult. I'm just gonna err on the side of caution. Ease bakery, right? I think I am gonna decorate this one like a cake. I'm going to get some modeling paste and pipe out on top of this with like cake piping bags. I think it'll look super fun. Turn my terrazzo into a cake. This one I'm gonna leave as a finish because I'm digging it. It's very, not the norm, but I'm all right with it. The alarm today was for the fire system yesterday. The sirens for a tornado. They actually come to me and sit under my feet or go to Jeff when there's a really loud noise. So it's fine. And so I gotta let those cure before I do anything else. See if I can find the littles. Canvas, what are you doing? Canvas! Okay, get my bobo. Get my canvas. Look how big she is already. She's like bull grown baby fox. Anyways, Boog, I didn't know if you saw Canvas yet, but you remember my bobo. I wanna have to show off this cup that Laura Taylor got for me. Look at the baby moo, my bobo. She sent me that before we got Canvas. Anyways. So I'm gonna go before my alarm system kills your ears. Remember that piece? Gray, gray, there's gray in that. Been doing a lot of finishes lately. Oh, that's awesome, Deborah. Yeah, she was definitely bigger than last week. Anyways, I hope you guys have enjoyed this video. You've learned something, we're entertained or otherwise educated on stuff that we do. If you have any questions, please leave them down in the comments below this video. If you like anything you see here that I don't sell in my shop, which is artist-to-let.com, then you can find it down in the Amazon link below this video. Doink, doink, doink. I hope you enjoyed it.  Sweet, thanks. And it won't be live tomorrow. We've got a class here in Dallas this weekend. If you'd like to join the class, I have some seats that are still vacant. You can register on my website, artist-to-let.com. You can also get this glitter and many others on my website at artist-to-let.com along with these colorants. And yeah, I hope you guys have an awesome evening, an awesome safe weekend. Happy Little Green Manday. And be kind to one another because you never know what someone's going through. And now I draw a memory. Sorry, bubba. We do the test, so you don't have to. Did you say so we don't have to? I heard you. You're amazing. Bye.


So one of the, one of the things that we do at my podcast is called the best life podcast. And one of the things we talk about a lot is honest communication.  And I find that it's so hard for people to honestly say what it, what's on their mind to their partner or to their coworker, to their boss or whatever, because they're constantly worried about how it's going to be perceived. And to your point, like telling someone they need to change is never going to be met with positivity, right? But you can state your preferences and it's not so that they'll agree with you. It's so that they can know, right? Because if you don't tell them, they don't know, and they may or may not be able to take it on. They may or may not be able to change those results, but it's for you to practice stating your needs and then just letting the chips fall. And so I have like a really, I don't know if you guys are interested, but I have like a three S system that I teach my clients and it's how to have an honest conversation to bring up something that you are, you know, kind of swallowing and not allowing yourself to bring up in a relationship. So the first S is set the stage. This is when you tell them that you're really, that you're feeling vulnerable, that you're kind of scared to bring this up because you're worried about their response. You're worried about how they're going to take it. You don't want to hurt their feelings, but you really want to bring up something and you feel really vulnerable. So when you do that, you kind of get them on your team ahead of time versus like in the heat of an argument being like, you need to do this. Like that's never going to be like what it's high emotion. So find that place when it's like emotionally neutral and say, Hey, I really want to talk about something. I've been a little bit nervous to bring it up because I'm just, I don't want to hurt your feelings, but I feel like I really want to share it with you. Get some on your team a little bit. It's like an ice breaker. The second S is just state your truth as, as clearly as you know it in that moment, because we know that truth is evolving. Like we just know like what we feel in the moment now, you're going to state it as clearly as you possibly can. And then the last part is the hardest part and that is stick it out. Stick it out.  And so, because that's the whole part of it is trying not to change what you're going to say to consider them to state it as clear as you can. And to me, honesty is a service, honesty without cruelty, right? Honesty with empathy. But I think that honesty is inclusive. I think when you talk about affairs, that's the part that is the most hurtful. It's not the actual physical act. It's the exclusivity. You didn't have all the information. I wish that I had known when it was going on. So I would have had a choice in the matter. And so to me, honesty is inclusive and it's a gift. It's like the best thing you can do to the people that you say you love. And I think the important thing to realize is that everyone processes that truth differently. And a lot of times when we, you know, truth bomb someone, we share what we're feeling, their reaction, if it doesn't go as planned, you know, can frustrate us and we become very selfish and like, well, no, I want you to hear this and I want you to change and realize that some people are going to take it very silently and they're going to have to process it. Maybe not in the moment. It may take days, weeks, even months for it to fully hit them. Some people are going to be very reactive to it and maybe even blow up, but it doesn't mean you're not heard. And I think that's, we oftentimes we project what we expect is going to happen. And then when it doesn't happen, we're like, oh, I shouldn't have done that. I shouldn't have brought it up. I'm just not, see, I can't even bring it up. And I know, you know, throughout my relationship, Amy has been honest with me and then honest when my reactions have disappointed her and I've had to explain, like, this is how I process the information. And I just want you to understand that the behavior change and the actions that follow in the days, weeks and months after are a lot more important than my reaction in the moment. Because the way I was conditioned and grew up is I was the passive recipient of anger and yelling. So me shutting down and processing is just how I've come to deal with it. It doesn't mean I'm not going to change. And it certainly doesn't mean I'm not listening. Yeah, there's been, I mean, we've been working together for 12 years and obviously not everything is going to go easily, especially in the beginning when you're duking it out just to try to create something and make something happen. But, you know, in those times, there's been conversations we had to have with each other. And it's like, it always, we would always do this though, the one thing, it's like, listen, I'm going to tell you some things in the moment right now. You do not have to answer. And I'm not expecting an answer. In fact, all I want is for you just to hear what I'm going to say and take a few days with it. And then let's have a conversation about it. And it's like, here's what I'm thinking. It's like, I don't like any what you just said. So, but I'm going to take it home and I'm going to stew on it. And then it's like, you go through the emotional theater and it's like, you get worked up, but then you're able to work through it. It's like, okay, there's a point there. I have an argument for that, that piece there we need to discuss. And it's like, well, great. We are now moving beyond just those initial feelings. And now we can get to somewhere where we're both happy. Because if two people sit down and are adults and can communicate and articulate their issues, then there should be a win-win here. And if we're fighting for the same thing, then it should be easy for us to find that. Yeah, I think it's one of those things where you have to like the first time that you have that really uncomfortable. And I love you guys have, obviously you worked together for such a long time. You've had those instances every single person I'm close with in my life, we've had those moments of like, I could easily not bring this up, right? I could easily like not do the thing. But when you engage in that difficult kind of awkward even conversation at times, it really does deepen the connection. And it really, and it's a trust builder in the relationship, like I know that you won't run away from, like, I don't want you to be resentful. I trust that what you tell me, I take what you say at face value. And that's a huge trust piece.


end of day one. Only a short day but a big accident and a favorite for this Bentley hopper he's out of the rally. A little bit unfortunate he's taken out a power pole and a water main and the binds were across the road so the rest of the competitors couldn't come through but they're alright I guess that's the main thing. It's a great day in the Igualdo de Ivo just a little bit in front of Tony Quinn and Jason Gill is right here as well although he has had some gearbox problems they're going to change that tonight and he'll be back at full speed in the morning. I'll be keeping my eye on him because he goes very fast it's a big day down the road. That was the Andrewsons full-wheel drive category. In modern the paceman is Clark Proctor. This year again everything's replaced and the car feels strong and there's less mechanical noise in the car than there ever has been ever from day one so it's really really strong and today to be honest I just took it quite easy and just went up the middle of the road I did quite nicely so he's happy how I'm driving and it's a real good start like a real great start. The big story in the classic class is not all about Kirk and Ant and BMWs. We have Jason Easton and Bevin Claridge an American-Australian muscle doing some fantastic times and leading the way it's going to be a good battle between those two tomorrow and to see if the BMWs are the tighter twistier stuff and start to pull their way back. Speaking of V8 there's a certain little fear that it was trying to chase one down and it didn't end well. I was trying to catch a Corvette that was in front of us and we could see him and yeah I just overcooked it really. Going too fast in the corner and locked up halfway through so bad result.


Welcome back to a new session on dentistry and world. Today's topic is Ashley's analysis. So this analysis is peculiar because the tooth crowding, the analysis pointed to the arch width rather than arch length. So we have seen various analysis and one of them is Ashley House analysis and it stressing upon arch width not arch length. The tooth crowding is due to deficiency of arch width and not arch length. Most of the analysis we are seeing was due to the deficiency or discrepancy in the arch length. So Ashley House analysis stressing upon her relationship existing between the total width of mesiodisital diameter of teeth anterior to the second permanent molar that is mesiodisital diameter of teeth anterior to second molar that is first molar to first molar mesiodisital diameter and the width of dental arch in the first premolar region. The width of dental arch in the first premolar region. So this is a width of dental arch the first premolar region this is just diameter this is basal arch width. So it is is tricking the width of crown and root epises from first premolar to first premolar on left and right side. So the author pointing out there is a relationship which existing between the total width of mesiodisital diameter of teeth anterior to second permanent molar that is first molar to first molar and with the width of dendel arch between the first premolar to premolar that is between the first premolar region arch width and the total width of mesiodisital diameter. So in this analysis we have four parameters and the analysis is exclusively based on these four parameters. The first one is total tooth material. Total tooth material is nothing but the sum of mesiodisital width of the teeth from first molar to first molar. So that is the sum of mesiodisital width of first molar to first molar. Okay that is total tooth material. This is total tooth material and the second one is basal arch length. So this is basal arch length. So it is a perpendicular distance. It is a perpendicular distance from a tangent. So tangent is from the first permanent molar distal side. So this is a distal aspect. I just mentioned it as tooth number, central lateral canine, first premolar, second premolar and first molar. So the distal aspect of first molar we draw a tangent and draw a perpendicular to the anterior limit of arch. So that is basal arch length. It is a perpendicular distance from the tangent drawn from the distal aspect of first permanent molar. That is BAL or basal arch length. The third parameter is premolar diameter. So premolar diameter is arch width measured from the buccal cusp of first premolar on one side to the buccal cusp of first premolar on the other side. So that is buccal cusp of first premolar to the buccal cusp of first premolar on the left and right side. So that is the premolar diameter or PMD. So we are checking buccal cusp. So that is the arch diameter or premolar diameter. So the last one is PMBAW. There is nothing but premolar basal arch width. So here we were checking the buccal cusp from first premolar to first premolar on the left and right side. But this PMBAW we are checking the root epises of first premolar to first premolar. That is premolar basal arch width. This is buccal root cusp and this is root epises. So these are the four parameters of Ashley House analysis based upon the analysis of final results will be assessed. The total tooth material basal arch length premolar diameter premolar basal arch width. Now let us move on to the percentage calculation of premolar diameter to total tooth material. It is nothing but premolar diameter divided by total tooth material into 100. If it is PMBAW that is premolar basal arch width divided by total tooth material into 100 or basic arch length divided by total tooth material into 100. So all this percentage we can calculate and we commonly express the result or commonly say with the premolar basal arch width. Suppose the premolar basal arch width to total tooth material percentage is less than 37 percentage. So we should do extraction or it requires extraction so that particular patients cast after analysis after checking premolar basal arch width and total tooth material and we get a value which is less than 37 percentage that patient requires extraction. If the value is between 37 to 44 percentage it is like a borderline case and it may or may not require extraction depending upon other parameters that is 37 to 44 and if it is greater than or just 44 not greater than just 44 it is an ideal case extraction is not required. That is a percentage wise analysis and the next thing is if premolar basal arch width is greater than premolar diameter that is the root apacus dimension is greater than the crown diameter that is this is a root apacus diameter root apacus width and this is diameter of crown to crown. So this is greater than the premolar diameter the basal arch that is the arch of the patient is sufficient to allow expansion that it has enough room to give expansion for the teeth that is basal arch width is greater than premolar diameter and if the opposite happens that is if premolar diameter is greater than premolar basal arch width there can be three possibilities the one is which is contraindicated for expansion the expansion will not will not happen and we should not perform any expansion procedure and there are chances of moving the teeth distally the distal movement of teeth may occur and we should go for extracting some teeth so that is under this case so that is all about ashley house analysis it is based on the arch width concept not the arch length and it has four parameters total tooth material basal arch length premolar diameter and premolar basal arch width so that's all about ashley's analysis so i'll come up with which separation in my next video thank you


Hi everyone, Joe for jaspyscasebrakes.com. Coming at you with a quick little pack break right here, 2019, 2020, Panini Chronicles MBA Fat Pack from jaspyscasebrakes.com. It's close to the end of my week, folks. Losing my voice. Now, you're thinking each spot is 36.99? That doesn't make sense for one pack. Well, because we're giving away seven mixer spots in here. Where's the mixer? Where's the mixer on jaspyscasebrakes.com? There it is. Where we're giving away a Luca? What? For a fraction of the, you can, for that price right here, you can get that spot right here and try to get that guy right there. That's pretty nice. All right, so, but as always, we gotta do the break itself first. So let's, those are the names. Thank you. Those are the teams. Let's roll it. Let's randomize it. Four to four, eight times for each list. Hard eight, one, two, three, four, five, six. Seven, and eighth and final time. Sean Jaspy almost has a stroke over Wild Wing falling in the fire pit. He's like, no Wild Wing. No. Nick Stober down at Josh Melton. Not Wild Wing clutching his Wild Wing plush doll. Right Sean? Four to four, eight times for the teams. One, two, three, four, five, six, seven and eighth and final time. New dice roll for the giveaway. Grizzly is down to bulls after eight. All right, there's the first half of the list right here. Grizzly is for Nick Stober. Could be some John Morantz in here. Where are the Palakins? There are the Palakins for Chris Hoyt. Miami Heat, Kim. So there could be some interesting things here. All right, so let's roll it, or not let's roll it, alphabetize it and let's go ahead and do the break. I already rolled it. Long week, folks. All right, let's see what we got here. There's a Kendrick Nun, there's a LeBron James. Those are pretty cool. There's a John Morant. There's a Yanis, that guy's pretty good too. There's a Luca, there's an Anthony Davis and a PJ Washington. Not too shabby, there we go. And there's the, once again, the final printout right there. Nice, even the second year Luca Donchich cards do pretty well on a secondary market. So does Yanis. So does champion LeBron James. And that playbook design right here for the Lakers, that goes to Nicholas. Kendrick Nun for the Heat, for Kim. And then a nice John Morant for Nick Stober and the Grizzlies. That is strong, right there, excellent. Now let's see who's gonna win spots. So let's flip back to this list right here. Let's grab everyone from James O down to Chris. And it's gonna be the top seven after nine. Top seven after nine. One, good luck. Two, three, four. And ninth and final time. After nine, everyone from eight down to 30. We apologize, we'll get them next time. In fact, we have to do one more of these packs plus salads and straight up spots. But we gotta do one more filler to help fill this break. Maybe try again. Congrats to the top seven. Daniel, James, Nick, Robert, Kim, Jesse, and Kim. There you go. Congrats to the seven here. Thanks for joining the break, the quick little filler pack break. One more we have to do. Check it out, jaskyscasebreaks.com. Thanks, buh-bye.