Good morning. Love it. The commencement ceremony for criminal justice, cyber security and networking, forensic science, legal studies, leadership and public administration programs of the School of Justice Studies will now be in order. Please be seated. Good morning scholars. I guess only a few of you were in my class. Good morning scholars. Recently I was informed that I would be speaking at this event so naturally being a good researcher I went ahead and looked up tips on how to give a proper commencement speech. Keeping in mind that Google is a search engine and not an answer engine. A plethora of tips popped up on what I'm supposed to do here today. For example, turning to our friends from the social sciences in an effort to feel more confident while I speak to you, I should strike a power pose. Next, I should dress in a way to feel comfortable. As I'm sure you can imagine this is something I wear whenever I'm trying to relax around the house. Another common tip is to practice in front of a mirror or in front of a few loved ones. My poor children have heard this speech seven times. Obviously I'm joking. They bailed halfway through the first run. Another common tip on how to give a commencement speech is for us to all consider the past. I read that I was supposed to encourage you to think about the growth that has occurred over the last four or so years. The events couldn't bad that have shaped you. Recall what has taken place during these times. Clearly your experiences while here at Roger Williams earning your degree were impacted by a little thing known as COVID-19. Your educational experiences have been dramatically different than anyone else who has received a degree prior to 2020. You spent time at home. You spent time on Zoom. You watched recorded lectures. We sent you messages through emails, bridges, and posts on our RWU web page regarding the changes to expect. And here's how we are going to live moving forward. Two years ago, the upper administration approached the deans and asked them to prepare videos on the expectations of reopening Roger. I'm sure you all remember watching me. I think there was like a 10% open rate on that video. As a side note, I did try and point out to them that some of us deans were better built for radio. In my message, I spoke to you about the word unprecedented. Unprecedented was being thrown around in 2020 to describe what we were experiencing. We would hear and read over and over that we were living in unprecedented times that you were experiencing an unprecedented phenomena. Well, today I'm here, I would like for you to consider the word persevere. According to the Oxford Dictionary, 2021, we always want to cite your work, right scholars? To persevere, which is a verb, means to continue on a course of action even in the face of difficulty or with little or no prospect of success. When we return to campus in the fall of 2020, there was still much that was unknown about the COVID-19 virus. I think we can all agree the unknown is a scary thing. This was just, this was not just an unknown of whether or not you were going to be able to pass a class. We're trying to figure out if you were going to get a particular job after graduation. The unknown we were dealing with was society going to make it. Were humans going to be able to survive? And in the face of this unknown, in the face of fear that we were all dealing with, you persevered. During these unprecedented times, you continued on a course of action in the face of difficulty and you succeeded. In the cynical world in which we live, negativity is loud. You can hear something, right? Hurry up, Dean Bronson. It receives attention. Negativity has tremendous power and reach in our society. All you have to do is look around and you'll see it on the news. Social media as we know thrives on it and negativity is something that is loud. On the other hand, positivity is quiet. At times even look down upon. Celebrating a positive outcome is often looked down upon. Society will say you need to be humble. Positivity is sprinkled throughout society like a spice that should not be overused. When you watch the evening news, nearly the full show is spent on awful phenomena with a 30-second piece on some kid that raised money for a charity by selling lemonade. I think we can all agree there's not enough positivity in this world. I would like to encourage you to celebrate the positivity in your life. This is without a doubt one of those times in your life that you should celebrate. Celebrate that you persevered. Celebrate that you succeeded by continuing on that course of action in the face of difficulty. Celebrate with those in attendance with you, your family and friends. Thank them now. Turn and look. Thank your parents, family, friends. Yeah, there you go. And how about thanking this wonderful faculty up here who persevered along with you because, trust me, they didn't sign up to teach that way either. So that wasn't the experiences they were looking for. In an effort to keep this short, one of the final tips for a proper commencement speech suggested that I read a quote, staying with the theme of persevering. I encourage you to wake up each morning and tell yourself we are going to attack the day with an enthusiasm unknown to humankind. Congratulations, scholars, you've done it. I would like to ask Professor Tricia Martlin to come forward for the presentation of the President's Core Value Medallion honorees. Thank you. The President's Core Values Medallion has been established to recognize graduating seniors who best reflect university core values through distinctive contributions supporting the core purpose of a Roger Wim's university education to strengthen society through engaged teaching and learning. It is truly an honor to announce the Core Values Medallion recipients of the Class of 2022. And may I just add, I saw one of them for the first time without a mask on this morning. How wonderful. May I introduce Amanda Neff to introduce our at-large recipient, Isaiah Berrientos. These students have exemplified our core values through inclusive, innovative, engaged, experiential, and transformational academic and co-curricular achievement throughout their years. Congratulations to our recipients and their families, friends, and faculty. Next, I would like to ask Ms. Madison Berri to the podium to deliver the 2022 School of Justice Studies Student Commencement Speech. Maddie? Good afternoon, family, friends, faculty, and fellow graduates. I'm honored to be standing here today as a reflector of what the School of Justice Studies Department has accomplished these past four years through all of our hard work, dedication, and commitment to better the university, the justice system, and overall society. I want to start off by recognizing the profound dedication every single student sitting here today has exhibited in completing their degrees. Personally, being a first-generation college student has allowed me to explore the many trials and tribulations that college has to offer. Even with the passing difficulties of trying to figure out what it means to be a college student, the School of Justice Studies has transformed my experience by educating me in a proper way. These experiences, whether personal or academic, have influenced the way I view my place in the system, and I am hopeful that every person sitting here today feels the same. I believe that every individual is here to better the justice system in the lives of those impacted by the system. Whether it's as a forensic scientist developing new ways to analyze DNA for exonerations, a police officer giving up their family lives to work overtime, a cybersecurity analyst protecting data and information important to the security of our country, an attorney protecting individuals' alienable rights, or even a victim advocate serving as a protector of victims within the justice system. Let's also not forget about the ROTC students that are headed out into the military where they will protect and serve our country so we have the freedom to live our lives. Shout out Lexi! The legal definition of justice says, justice is the fairness, moral rightness, and a schemer system of law in which every person receives his, her, its due from the system, including all rights, both natural and legal. Sorry, Bronson, I didn't cite this. Now, we must think to ourselves, what does justice mean to us as educated individuals? What is our role and responsibilities to ensure justice for those that do not have the capability or means to warrant justice for themselves? Throughout our academic journey here at Roger Williams University, we are now ready and able to apply these ethics and lessons that we have learned towards issues prevalent in our country's justice system. As the next upcoming generation of justice advocates, it is our job to use our learned knowledge and fix these issues for the better of our system as a whole. With the knowledge we have gained in regards to these ethics, it has also pressured us to address the gray areas within the field. It begs us to question the law in order to improve the justice system. While many people believe the law is black and white, I'm here to tell you it is not. Over the next few years, you all will encounter scenarios in which you will have an immense amount of discretion in order to control an issue. Eventually, you will all have to make decisions that you may be uncomfortable with. You will engage in conversations that you never thought you would have to be a part of. Therefore, I say this with confidence. Roger Williams University School of Justice Studies has provided us with the cognizance, knowledge and wisdom to approach the justice system with apprehension to progress towards a superior system that benefits all of those impacted by cell. Now, as I reflect on the stage in my life where my undergraduate degree has come to an end, I accept that I still don't know what I want to do. I do know that I am prepared to face the reality of the lessons that we have learned the past four years. I'm sure many of you would agree with me that this is the most difficult part of the college experience.  I'm here to say that it's okay not to know. With this degree, we will all explore different opportunities that the justice system has to offer. We will live and we will succeed. I want to end with a quote from Ruth Bader Ginsburg that emphasizes our part in the justice system as we continue to learn, change and progress towards our hopes of an exceptional society. We live in an age in which the fundamental principles to which we subscribe, liberty, equality and justice for all are encountering extraordinary challenges, but it is also an age in which we can join hands with others who hold to those principles and face similar challenges. With this being said, I would like to wish you all luck in your future endeavors. Congratulations to the 2022 class of justice studies. Thank you Maddie. I do want to point out though, when you got to that point in your speech where you said you still aren't sure what you want to do, you remember that part? I looked at your family and the total looks of terror. We now proceed to the chief purpose of these commencement exercises, the conferring of academic degrees upon the class of 2022. Our undergraduate students today wear a hood signifying their graduation with their bachelor's degree and permanent connection to Roger Williams University community. These hoods bear the RWU colors of white and light blue. Students receiving their master's degree today will be hooded by a member of the school faculty as they process for their degrees. The formal hooding of students receiving advanced degrees in a century's old practice and signifies students achievements in their chosen field of study. Master's students hoods include wide velvet bands in the color denoting the academic area in which their degree is held. We would like to also acknowledge those students who have the distinction of graduating with honors. Would all students graduating cum laude with a cumulative GPA of 3.4 please stand if you are able? Will all the students graduating magna cum laude with a cumulative GPA of 3.6 please stand if you are able to be acknowledged? Finally, will the students graduating summa cum laude with a cumulative GPA 3.8 please stand to be graduated? Your hard work and commitment to academic excellence are to be commended and we are proud of all of your accomplishments. We will now begin the conferral of degrees. Will the School of Justice Studies candidates please rise just like we practice? Great. I am pleased to certify that these candidates are eligible for the degrees appropriate to their course of study. By the virtue of the authority granted by the state of Rhode Island and delegated to the president by the board of trustees, we confer upon you the degree to which you are entitled with all the rights and privileges pertaining to th- thereto. Will the candidates please come forward? Stephanie Marie Jimenez, Master of Science Criminal Justice. Matthew David Thompson, Master of Science Criminal Justice 4 plus 1 program. Nicholas David Polinsky, Master of Science Criminal Justice 4 plus 1 program. Anya Gabrielle Bornstein, Master of Science Criminal Justice. Mia Salerno, Master of Science Criminal Justice 4 plus 1 program. Kelsey Lynn Poison, Master of Science Criminal Justice. Emily Batello, Master of Science Criminal Justice 4 plus 1. Samantha Marie Mancinelli, Master of Science Criminal Justice. Jonathan Warden, Master of Science Criminal Justice. Jennifer Delafred, Master of Science Criminal Justice. Austin James Cloutier, Master of Science Cyber Security. Sean Christopher Pulver, Master of Science Cyber Security. Elijah A. Kloizek, Master of Arts, Juris Doctor, Cyber Security. Casey A. Boseman, Master of Science Leadership. Bragg Lee S. Brown, Master of Science Leadership. Steven M. Lulibiti, Master of Science Leadership. Alexander Matthew Lucini, Master of Science Leadership. Mary Rosina Centuro, Master of Science Leadership. Tracy Michelle Pratt, Master of Science Leadership. Sia N. Kornger, Master of Public Administration. Price Philip Johnson, Master of Public Administration. R. M. Smith, Master of Public Administration. Paul Glenn Vario, Master of Public Administration. Christina G. Almeida, Master of Public Administration. Helena P. Montero Silva, Master of Public Administration. Janice L. Silver, Master of Public Administration. Wendy Hawk Vergadamo, Master of Public Administration. Heather Kinsey Trotman, Master of Public Administration. Kimberly May Tebow, Master of Public Administration. Now we're going to move on to our undergraduates. Madison Taylor Birdie, Bachelor of Science, Criminal Justice. Katherine Theresa Bossi, Bachelor of Science, Criminal Justice, and Psychology. Nicole Julia Norton, Bachelor of Science, Criminal Justice, and Psychology. Andrew Jared DeClement, Bachelor of Science, Criminal Justice. Abigail Maddon Smalley, Bachelor of Science, Criminal Justice. Katrina Genevieve Garrity, Bachelor of Science, Criminal Justice, and Psychology. Madeline Holland Duggan, Bachelor of Science, Criminal Justice, Magna Cum Laude. Nathan Joshua Douglas, Bachelor of Science, Cyber Security and Networking. James Elliott Shapiro, Bachelor of Science, Criminal Justice, Cum Laude. Tyler LaMere, Bachelor of Science, Cyber Security and Networking, Magna Cum Laude. Grace Di Amato, Bachelor of Science, Criminal Justice, Summa Cum Laude. William Flanagan, Bachelor of Science, Legal Studies, and Political Science, Summa Cum Laude. Casey Dean Litchford, Bachelor of Science, Cyber Security and Networking, Summa Cum Laude. Jake Joseph Malatesta, Bachelor of Science, Criminal Justice. Thomas Leo Myers, Bachelor of Science, Criminal Justice. Kayla Ann Ferrante, Bachelor of Science, Criminal Justice, Summa Cum Laude. Samantha Jane Pollan, Bachelor of Science, Criminal Justice, Cum Laude. Mark Anthony Brochu, Bachelor of Science, Criminal Justice, Cum Laude. David Manuel Marshall Jr., Bachelor of Science, Criminal Justice, Magna Cum Laude. Edward John Gogan, Bachelor of Science, Criminal Justice. Katie Ann Devere, Bachelor of Science, Legal Studies and Psychology, Cum Laude. Noah George Leolos, Bachelor of Science, Legal Studies and Psychology. Zachary Collins, Bachelor of Science, Cyber Security and Networking. Peter Joseph Frost, Bachelor of Science, Cyber Security and Networking, Cum Laude. Stephen Anthony Fiume, Bachelor of Science, Cyber Security and Networking. Tyler John Bruehler, Bachelor of Science, Cyber Security and Networking. William Joseph Marta, Bachelor of Science, Cyber Security and Networking. Hai Van Lee, Bachelor of Science, Cyber Security and Networking, Summa Cum Laude. Allison Jean Coots, Bachelor of Science, Criminal Justice, Psychology, Magna Cum Laude. Bethany R. Gartner, Bachelor of Science, Criminal Justice. Jacob Wright-Belsarini, Bachelor of Science, Criminal Justice, Magna Cum Laude. Daniel P. Griffiths, Bachelor of Science, Criminal Justice. Patrick James Nagel, Bachelor of Science, Criminal Justice. Jonathan John Walter Wittison, Bachelor of Science, Legal Studies and Philosophy. Vincent Eric Ronda, Bachelor of Science, Criminal Justice. Jonathan T. Canney, Bachelor of Science, Criminal Justice. Andrew James Dawson, Bachelor of Science, Criminal Justice. Cassidy Lynn Donovan, Bachelor of Science, Criminal Justice. Zachary M. Domian, Bachelor of Science, Legal Studies and Public Relations. Taken David Welch, Bachelor of Science, Criminal Justice. Leo Robert Kaiser, Bachelor of Science, Criminal Justice. Megan Colleen McNally, Bachelor of Science, Criminal Justice. Madison A. Medina, Bachelor of Science, Criminal Justice. Julia Grace Schmidt, Hogan, Bachelor of Science, Criminal Justice and Psychology. Alexander Renee Welch, Bachelor of Science, Criminal Justice, Psychology, Magna Cum Laude. Sarah Rose Jammeros, Bachelor of Science, Legal Studies and Psychology, Magna Cum Laude. Noah Timothy Allen Morbido, Bachelor of Science, Criminal Justice, Summa Cum Laude. Jaden Michael Joseph Baker-Decosta, Bachelor of Science, Forensic Science. Emily McKenzie-Marie, Bachelor of Science, Criminal Justice. Kaitlyn Ann Caboni, Bachelor of Science, Cyber Security and Networking. Stephanie Victoria Dos Santos-Borba, Bachelor of Science, Criminal Justice. Gianna Nickerson, Bachelor of Science, Criminal Justice. Jaya Mitchell, Bachelor of Science, Criminal Justice. Erica Taylor Short, Bachelor of Science, Criminal Justice. Savannah F. Bonofsky, Bachelor of Science, Criminal Justice, Summa Cum Laude. Haley Mendez, Bachelor of Science, Criminal Justice. Prashant Anand Kivali, Bachelor of Science, Criminal Justice. Megan Ellsworth, Bachelor of Science, Criminal Justice, Cum Laude. Madison Alexandria King, Bachelor of Science, Criminal Justice. Alexis Violet Field, Bachelor of Science, Criminal Justice, Cum Laude. Andrew Zapatella, Zampatella, Bachelor of Science, Criminal Justice. Louis John Fasaro III, Bachelor of Science, Legal Studies and History, URA, Owen Peter Mousseau, Bachelor of Science, Criminal Justice Psychology, Summa Cum Laude, Ashlyn Keaton-Whitty at Bachelor of Science, Forensic Science, Summa Cum Laude, Ryan Adam Burt, Bachelor of Science, Criminal Justice, Jack Gregory Lambrose, Bachelor of Science, Criminal Justice, Emily Simonin, Bachelor of Science, Criminal Justice, Natalie Marie Hinton, Bachelor of Science, Criminal Justice, Kirk Hanson-Schultz, Bachelor of Science, Forensic Science, Bridget Marie Mary Woods, Bachelor of Science, Forensic Science, Andrew J. Skemperis, Bachelor of Science, Forensic Science and Public Health, Cum Laude, Cameron James Quinn, Bachelor of Science, Criminal Justice, Emily Ruth DeVarquez, Bachelor of Science, Forensic Science, Cum Laude, Austin Batello-Cabral, Bachelor of Science, Legal Studies and Political Science, Magna Cum Laude, Elena May-Vantine, Bachelor of Science, Legal Studies and Political Science, Summa Cum Laude, Lauren Elizabeth Heider, Bachelor of Science, Legal Studies and Political Science, Erica Marie Britell, Bachelor of Science, Legal Studies and Political Science, Jordan Keating-Codus, Bachelor of Science, Criminal Justice Psychology, Magna Cum Laude, Daniel Joseph Martin, Bachelor of Science, Criminal Justice. Kara Bryn Garrett, Bachelor of Science, Forensic Science. Michelle Elise Caria, Bachelor of Science, Criminal Justice, Cum Laude. Priscilla Cristobal Marakwin, Bachelor of Science, Forensic Science, Cum Laude. Walter Monroe Friedrich, Bachelor of Science, Cyber Security and Networking. Jessica Ann Castello, Bachelor of Science, Forensic Science. Nico Nozaki, Bachelor of Science, Forensic Science. Bachelor of Science, Criminal Justice, Cum Laude. Katerina Angelic Tidoccio, Bachelor of Science, Criminal Justice, Cum Laude. Bachelor of Science, Criminal Justice, Theater, Summa Cum Laude. Sanchez, Bachelor of Science, Legal Studies, English Literature, Magna Cum Laude. McClellan, Bachelor of Science, Criminal Justice, foreign language cum laude. Kaitlyn Emily Wright, Bachelor of Science in Indigo Breaches, Bachelor of Political Science cum laude. John Manning, Bachelor of Science, Criminal Justice cum laude. Bachelor of Science, Legal Studies, Political Science summa cum laude. Marco Centonio Mazza, Jr., Bachelor of Science, Criminal Justice. Roberto Sabatino Cucci, Bachelor of Science, Legal Studies in Foreign Language. Michael N. Calandro, Bachelor of Science, Criminal Justice. Kayla E. Natalo, Bachelor of Science, Criminal Justice, cum laude. Jiswalde, Jiswalde. Emma Jiswalde, Bachelor of Science, Criminal Justice, magna cum laude. Jelica Chila, Bachelor of Science, Criminal Justice. Bachelor of Science, Forensic Science, cum Rocha. Bachelor of Science, Forensic Science. Alexis Sienna Ely, Bachelor of Science, Forensic Science, cum laude. Christian Thomas, Bachelor of Science, Criminal Justice. Trinity Marie Potter, Bachelor of Science, Criminal Justice. Selvamille Alfonso, Bachelor of Science, Legal Studies, English Literature, Magna Cum Laude. Zahiella Yanis, Hawi, Bachelor of Science, Legal. Tasha Jackson, Bachelor of Science, Criminal Justice. Congratulations, scholars. Just one little thing before we decide to head out, we have to make sure we take care of social media, right?  Just remember, took your four years to get your degree. You're all set to go. All right, on behalf of the university, thank you all for joining us here today and celebrating commencement exercises. Please stand and remain at your seats until all the members of the recessional, including the graduates, have exited the tent. Congratulations once again. Thank you.


Hi everyone, welcome to the proteomics hands-on training. In this demonstration, I will show how to use MaxQuant and MSDOTs for the analysis of a label-free skin cancer tissue cohort data set. I will use the European Galaxy server and whatever server you use, I really recommend to use the training material inside the Galaxy server. You can find it here at this graduation head, simple. When you click on it, you end up in the training website and you can navigate to the MaxQuant and MSDOTs training site. And this has many advantages which we will see during the training. So we will immediately start to upload the data because it might take some time and I will explain a bit more about our training while we are waiting for the files to upload. So part of our files is deposited in Synodo and we can just copy the links to them by clicking on this copy symbol here. And then we go to upload data and paste the links in here. Press start and close. We can give our history a name, those are MaxQuant and MSDOTs label-free proteomics training. And we also have data on the Pride database. So this is an open repository for proteomics raw data. And as we use real live data sets, we copy the raw data from there. We can do this with this button again. Go to upload data and paste in these links. It's important that they have the right file types. So to make sure that they end up with the right format, we can check here, click on terminal.raw. And now all of these files automatically are type terminal.raw and that's the right type in Galaxy for terminal raw files. So we can also start this. This will take a little bit longer. And then we make this names a bit more beautiful. So I click on this pencil button and remove here the part of the link. So that it's called Protein Database Faster. And this is a faster file with all human protein sequences. And when we click on the i button to open the beginning of the file, we can appreciate that we have here the header line, which contains the unifrot accession, the protein name and a bit more information. And afterwards we have the amino acid sequences. And here's the second protein. And afterwards we have the amino acid sequences of the second protein and so on. And this we will use for MaxQuant, which does identification and quantification of proteins. Then we have an annotation file. So I also remove the beginning here. And this file is important for the MS-STATS analysis. It contains the experimental design set up of the experiment. So this file always needs to have five columns when we use it with a MaxQuant output. The first column contains the raw file name. And it's important that this name matches exactly the name of the raw files in the MaxQuant output. So we will later see that in our MaxQuant result, these are the file names that we see here in column one. Then we need to give the condition. So these are the groups that we would like to compare. In our case we have two types of squamous cell carcinoma. So we have a metastasizing squamous cell carcinoma. This is a cancer that occurs in elderly people after a lot of UV light exposure. And then we have a very special type of squamous cell carcinoma, which is the RDB and squamous cell carcinoma. So RDB stands for recessive dystrophic epidermolysis bolosa. And it's a very rare genetic skin blistering disease. And in these patients there are collagen seven mutations, which lead to deficient collagen seven. And one of the long-term complications that come with this rare disease is cutaneous squamous cell carcinoma. And this is quite an aggressive type of cutaneous squamous cell carcinoma. And we have six samples here, what is already quite a lot, because it's a rare disease. And we only got so many samples because these were formerly in fixed and paraffin embedded. And thus very easy to store at room temperature for longer times. In the third column we have the biological replicates. We have 19 different entries here. What means that we have 19 different patients. And because we have label-free no-fractionation data, we have also 19 runs in the mass spectrometer. And the isotope label type column is only necessary for max-quant runs in MS-STATS. And because we use input from max-quant for MS-STATS, we need this column. And because we don't have any heavy spike-ins, we label everything as light. Also for MS-STATS, we need this contrast or comparison matrix file. And this indicates which conditions we would like to compare. In our case, we only have two different type of conditions. And therefore, we only have one line here with the comparison of the metastasized versus the RDB, cutaneous squamous cell carcinoma. And here it's important that the names here and they had a line have to match exactly the condition names, how they were written in the annotation file. And this is only the comparison name, but it makes sense to name it according to the order of the comparison. So we compare the metastasized, which is indicated by 1, to the RDB, which is indicated by minus 1. So it makes sense that also our comparison name has the same order that it compares the metastasized versus the RDB, cutaneous squamous cell carcinoma. And if there would be more conditions, we would need to put every condition into a separate column. But if you are not interested in comparing each condition in every comparison, we could just use zeros for the conditions that should not be part of this comparison. Okay, yeah, these are quite a lot of files. So this might take a while, but I will try to already rename them. And here, it's important to rename them correctly. Because these names have later to match to the annotation file name. So if you do it with your own data, it's not so much of an issue because you would probably first run the max quant. And then after the max quant run, you would establish your annotation file and check that the names in the annotation file fit to the names that came out of max quant. In our case, now it's also not super important because the max quant run will take several hours. And we don't wait for it to run, we just take the max quant results, which are also deposited on Xenoto, and continue with these. So if you do a mistake here, it's also not not a big deal, because we will not continue with the max quant results we get from here, but we continue with the max quant results from Xenoto. And because we have so many files, it can be a bit easier to store them in a collection so that we have less entries in our history. And that's what I'm going to do afterwards after renaming them. So this first step, you could also skip because the max quant results are provided. And all those raw files consume quite a lot of space in your history. So you could just go theoretically through this max quant step. And if you want to learn more about max quant, there's a beginner's training max quant for the analysis of label free data. And there a lot of the max quant parameters are also explained in more detail. So I highly recommend going back to this if you are new to max quant. So it's always complaining that the metadata might not yet be changed because the file is not yet uploaded, but it should work here. So to create now a collection of all this raw data, I click on this icon here, operations on multiple data sets, I select all, but then exclude the three files that are not the raw files. And then for all selected, I build the data set list. And I can enter here now a name for the collection, I just call it the raw files. And I'm happy to hide all the original elements, because then I have less entries in my history. And I create list. And now it looks much cleaner. So all the 19 files are now in this collection. And when I click on it, I can see each of them. And when I click here, I get rid of the boxes in front of the elements. So now we start with the first tool, which is max quant. There are two versions of max quant. So for one, we can only specify the parameters in a max quant mqpar file. But with this tool, we can actually set parameters in this typical graphical user interface. So we have to choose which format our raw file has. And it's already selected terminal raw. But it would also be possible to do the analysis on open standard formats like mzxml and mzml. However, they have to follow a very specific format to be compatible with max quant. So it's not always easy to get the data into this right open file format. So first, we have to select the faster file that's already done for us. That's our first file in the history. And the pass rules, we can leave them as they are. So they specify which part of the header is of the faster file protein headers are kept and how they are split up to appear later in our output files. So max quant does identification and quantification. So here we have many parameters for the search options. We set the minimum unique peptides to one. What means that we are only interested in proteins that have at least one unique peptide, what is a peptide that only belongs to this protein and is not shared with other proteins. And then we set match between runs to yes. And this is quite helpful for labor free, larger labor free data sets, because we can now transfer identifications between runs when there's the same feature in another run, but it was somehow not picked for fragmentation. And so it didn't become an identification. It can get the identification from another file just by transferring the identity of the same feature. And now we have options for protein quantification. However, they are not so important because MS starts on the peptide level of the max quant outputs again and ignores all the protein level data. And here we have now to choose our raw files. So parameter group means that for different input files, we could choose different analysis strategies. But we would like to put all files into one parameter group and analyze them all with the same parameters. Because we have a collection, we click here on data set collections. And now we can select our history entry 23 with the raw files. We keep them as cleavages and also the modifications trips in as an enzyme. And in theory, we could set the quantitation method to label free. But it's not really necessary because as I said, MS starts will ignore the protein level quantification and all what happens with those label free quantifications that we get more accurate and normalized protein level quantification, which MS starts will later on anyway ignore. So we don't need to select it here. But we would like to generate a quality control plot with the pdxqc functionality. And the two output files that we need for MS starts as an input is the protein group file, and the evidence file. And I cannot start max one, but it's not really helpful or necessary because as I said before, this is a real life data set. It's not a crop and crop data set. It's a real tissue cohort. And so the max quant analysis will take several hours. And if you don't want to wait so long, you can just go into the training. And here behind the max quant, we have this box. And in the box, we have the max quant results. So we can copy them and upload them and continue with these files. So in the evidence file, we will see that we have the feature level data. In the protein groups file, we have the protein level data. And in the pdxqc, we have the quality report with a lot of different plots that visualize the quality and the properties of the data. And normally that's separate our package, but we have directly implemented it into the max quant in galaxy tool. Because it's just, yeah, it's made for max quant. And it's really helpful to get a quick overview of the data. So we can maybe rename it. Okay. So before we continue, we will have a quick look into the data set. So we need to wait until they are ready. So now the files are loaded into galaxy to open the PDF. I go to the scratch book and then click on the few button. And here we have already an overview about a lot of different parameters and how they perform in the different files. Don't be afraid to have something as red or even if many things are red, the cutoffs do not always fit for all experiments and all type of mass spectrometers. So we have an overview about the files and the parameters from max quant. Get like a PCA plot here. And this is a really important plot. So these are the contaminants. So max quant adds potential contaminant protein sequences to the faster file database. And we see here that up to 60% of our intensity derives from potential contaminants. But when we have a closer look at these contaminants, many of them are human and are skin proteins. And because we have analyzed human skin, we expect that these proteins are part of our sample and not a contamination. So in the next step, we will filter our files in a way that we can actually keep the potential contaminants for the statistical analysis in MS stats. So we have some intensity distributions. We have quite some as cleavages here, several mass spec parameters, how the mass spec performed, how well the match between run worked, looks good. And everything what is red is the amount of IDs that was transferred via this match between run function with the mass error. And here we see that the amount of identified MS2 spectra is counted as bad here. But actually for FFPE tissues, this is quite a common pattern that we see. And soon we see the peptide and protein identifications. And yeah, this looks quite okay. So it's between 1500 and 2000 from small FFPE tissues. So that's totally fine. Okay, then we have the protein groups output. Every line is one protein group. So we have 2621 protein groups here. And we can inspect it in more detail. Here's now all the protein level information about how many peptides we had in which group. If these were unique peptides or shared peptides, how many were there per sample. So this is quite a large file with many columns. Still per sample and the unique peptides per sample. And then we have some sequence coverage, weight, sequence length, the type of identification again for each sample, sequence coverage for each sample, intensities again for each sample, MS MS count per sample. And we are looking also for the potential contaminant column here it is. So every protein group that has a plus here means that this is a potential contaminant. And MS starts will in the first step remove all the protein groups that have a plus here. And exclude them from any statistical analysis. And because we expect that these are not potential contaminants but actually proteins from our skin sample. In the next steps we will remove only the non-human contaminants and keep all human potential contaminants. And unfortunately here the header is now currently not available in Galaxy but this should be column number 118. And we need this information later to know on which column we have to remove the pluses here from the human protein groups. And then the third file is the evidence file. This are 240,000 lines roughly and each line represents a feature. This means a peptide in a certain charge state with third modifications. So we have the sequences, modifications. And here's actually the raw file column. So if you do your own experiment it's important that the raw file name exactly as written in this column is the raw file name that should be in the first column of your annotation file for MS starts. Because this is like the key according to which MS starts matches the metadata from the annotation file to the proteomics data in the max-quant output files. So we have mass errors, retention time length, intensities. And here we have the potential contaminant column. And this should be column 54. So we later also filtered the feature level and removed the potential contaminant plus sign from all human proteins that were labeled as potential contaminant. So this is what we are doing now. So first we remove every protein and feature that is not human. Because these we consider are the real contaminants. And to do this we use the select tool and we can either look for it here but it might be a bit tricky to find so we go to the training material. Let's just reload. And here in this MS starts analysis sections we can just click on the tool and then it will appear and it will be there also in the right tool version. And I can already copy the pattern we are looking for before I click onto the tool. So I can copy the select pattern here. It means that we are looking for any lines that match either human or maturity. So human should be all the human proteins that we match and therefore keep. And maturity this word should only appear in the header line. So this is a way how we can keep our header line in the select line tool. And then we do the same for the evidence file. So in the evidence file the word sequence should appear somewhere in the header line. So we need to select here the evidence file from Zenodo. And with this step we also remove the features that derive from non-human proteins. And now for the human proteins which are the ones that we would like to keep we need to remove the plus sign in the potential contaminant column. And to do so we use the replace tool. And the protein groups are in our first file in file 30. And in the protein groups it should be column 118. And in this column we are looking for plus and in order to remove it we replace it with nothing. And then we repeat the same for the feature in the evidence file. So this is the second select file. And in the evidence file it should be column 54. And again we look for the plus sign and we remove it by replacing it with an empty field. And now to keep a bit track we can rename this file. So the first file was the protein group. And we name it now protein groups input for MS-STATS. And in this file we have now all the protein groups and it should only be human proteins but they should not be labeled anymore as potential contaminant because then MS-STATS would remove them. And the protein groups in MS-STATS are only needed in order to find out the protein inference but their quantities or abundances will be ignored by MS-STATS. And then we also rename the other file. So this is the evidence input for MS-STATS file. And now everything is prepared for MS-STATS. We can either search MS-STATS here or directly click on it in the training material. So there's two types of MS-STATS. MS-STATS TMT is obviously for label data and we use the MS-STATS in this version which is for label-free data. We first need to specify the input source. So we have max-quant as input. And now we need to provide the evidence in the protein groups. Of course we could use the ones that we obtained from max-quant but this would remove all those potential contaminant proteins. So here we use our filtered and manipulated inputs, the evidence and the protein groups. And we select the annotation file. It was here at the beginning. We select the leading razor protein column as an ID column. And by clicking on this max-quant to MS-STATS format options we have more filtering options for how the max-quant input should be treated when it is converted into an MS-STATS compatible table. And the only thing we change here is that we remove the proteins which have only one feature. And then if you're hidden in the data process options there are a lot of processing parameters. And what we will choose is also the run-level data as output and the sample quantification matrix. I don't think we need the raw data. And here you can see all those processing steps that happen before the statistical analysis. We do a log-2 transformation. We equalize for the medians. Yeah, we filled the rows that are not complete and so on. So here we impute the missing values. So we leave all those data processing options as they are. In the plotting options we select the QC plot, but we generate it not for every single protein, but only an overview plot that gives an overview about all the proteins. And to make it a bit more beautiful we can even go into the advanced visualization parameters, and there is a parameter about the angle of the labels and we set it to zero. And then we come to the statistical analysis. So to do a group comparison we need to say yes to compare groups and we need to select the comparison matrix. We obtain the comparison result as an output and to get a visualization we also choose the volcano plot and to make the volcano plot more beautiful we go again into the advanced visualization parameters and we say we use a 1.5 as a fault change cutoff and we don't want to display protein names in the volcano plot and that's it. So this will take a few minutes so it might be a good point to make a little break. Okay so now the msdots run is done. We can inspect the output files. The first file is the log file which captures the information about the data analysis steps and potential warnings and here we can also find the number of proteins and peptides per protein. Then we have the process data file which is a tab separated file with a lot of lines because it contains feature level data so we see for example the peptide sequence in the protein it belongs to and then all the metadata is attached to the features we see an intensity which is the raw intensity and we see an abundance value which is the log transformed and normalized intensity and we also find if the missing value imputation was performed for this peptide and then the features are summarized inside msdots to protein level abundances and this information is in the run level data that's again a tab separated file now it has a bit less lines but it's still a lot of lines because it has one line for each protein in each file or sample and on this protein level we have again the protein name intensities number of features so in the next step we will calculate how many feature a protein had on average it counts the missing value percentages and again all the metadata and another follow-up step that we are doing now is that we look at the file names and count for each sample or file how many proteins were used here to summarize how many proteins were now included per file into these statistical analysis so let's see so we look at this later so now we do the summary statistics on the run level data we use column four and in column four we had the number of features per protein and with the summary statistics we can have a look how many features were on average grouped into one protein and then we can already start the summarization of proteins per run so for this we use the data mesh tool and file information was in file eight so we go to the run level data we say we want to summarize file eight the file column eight we can confirm this here so eight were the different file names and we have a header line which we want to keep and we need to sort the input in order to be able to summarize then really all of the same file names after they were sorted and with this count we just count how many lines are summarized for each single file name and this gives us the amount of proteins per file so the summary statistics is already done it can be done on any numerical column and we find here a mean of six features per protein and we see that the median would be three features per protein but there is probably one or two proteins that have a lot of features so here's the maximum has 271 features that are summarized for one protein and here in the data mesh step we have now summarized our files that's why we have 19 lines so it has 20 lines one header line is 19 different files or samples and for each line for each file we have now counted how many lines had this entry and this corresponds to the number of proteins and we can now do a quick some visualization of this data with a bar diagram and we can here set more parameters let's do it here and now if we have the mouse over this we see that for the rdvcscc4 we have the lowest amount of proteins for this file okay so let's continue with the amistad's outputs we have a qcplot i might need this scratch book here to open it so this gives the distribution of protein intensities per sample and the medians here look all like on the same line what is a good sign and also the box plots are relatively equal and theory we could have done this also per protein then the pdf would have more than 2000 pages but then we could have inspected the data for each protein individually but for now this is enough we know that the data is relatively normally distributed after the log transformation and normalization then we have a file that is called the sample quantification matrix and here we have the protein id in the first column and then for every sample we have the abundance values in a separate column and we can now could look up for each sample the abundance per protein and we have something similar here so here we have the average value per condition for each protein so this information especially for the sample quantification matrix could be used in any other statistical or visualization tool for further analysis steps and then comes the most important table which is the comparison result table here we have again the protein id in the first column and then we have our comparison name but we only had one name so there's only the same label for every line then we obtained the log 2 fault change that gives us an idea how the abundances are different between the two conditions and therefore it's important that the name of your comparison fits to the actual order in which you compare the two conditions because a negative value now here means that the abundance was higher in rdb while a positive value means that the abundance was higher in the metastasized samples and here the most important column is the adjusted p value and we will now filter for this one but before we have a last output of m starts which is the volcano plot we have the log 2 fault change on the x axis and we have the negative log 10 of the p value what means that lower p values which means more significance are higher in this plot so higher values are better here okay so now we start to go on with this m starts comparison result which contains the statistical outcome so the adjusted p value column is column 8 before we do the filtering we can clean up the id a bit because it's if we have a look at the id here in between the two pipes we have the actual uniprot accession but before and afterwards we still have more information that we currently do not need anymore so in our first step we remove all this information so we take the comparison result as input column 1 and we want to remove this pattern but because this pipe character is also special character we need to actually put a backslash before it and we want to remove it so we put nothing into the replace field and we also want to remove the part behind the pipe so we say pipe and then we say we want to remove everything afterwards so that's the dot and the star and again we don't replace it with anything and now from this file we keep only the significant protein so now we click on the filter tool and there are so many filter tool that this is really helpful if the training is used inside the galaxy server because we just need to click on it and we know we have the right tool in the right version number exactly we use the replace and it was in column 8 and we would like to keep forward change p values below 0.05 and we have one header line here's the header line okay and before we continue we rename this file into the significant proteins file and then we filter once for default changes above 0.58 that means these are proteins that are higher in the metastasized condition at least 50 percent more abundance than in the rdb condition so these are the significant proteins and now we use the filter tool we can already copy this so we filter the significant proteins for the ones that are have a higher forward change in metastasized square msr carcinoma and then we do the same for below minus 0.5 that would be done the proteins that are high in rdb so we do the same filtering step again on the significant proteins and this time we keep only these and we rename and label them so these are the metastasized proteins that we don't get too confused by all these filtering steps and these are the rdb proteins and then we also can give them a tag we click on the tag simple and if we put a hashtag the tag even gets propagated and this is the metastasized and this becomes the rdb tag okay and we can already see that we have 134 lines here and 14 lines for rdb but if we have a closer look we can see that here's still one protein in which has a p value of 0 because it is missing in one condition and for now we will remove these proteins potentially they can be interesting so it might also be worthwhile to look into proteins that are completely absent in one condition but not in another but for now we will remove them and we do this by saying that the p value in column 8 has to be higher than zero because we already filtered for smaller than 0.05 we can now say higher than zero so this keeps everything above zero and below 0.05 and we do it with both the rdb and the metastasized proteins so I can click on the multiple input file and select both oops it should be column 8 and now we can do the same with the cut tool so that we only keep the id's we keep only the first column that contains the id's and for both filtered files we cut out only this first column and then we have the significant proteins id's for rdb and metastasized cutainous square massercasinoma and we would now like to combine them with the original protein abundances and to do so we also clean up the id protein id's of the sample quantification matrix so we do a similar step as before but this time we choose the sample quantification matrix in column one we would like to remove the thing before the pipe and we would also like to remove the pipe and everything afterwards and now the id's in the sample quantification matrix should look the same as here and then we can actually join them and this can be done with the join tool because it has exactly the same parameters and just the different input file one type the metastasized and the other type the rdb cut we can again run step six and seven at once so we use the here the replaced quantification matrix column one which has only the unibroad broad accessions and here in the second file we can use the multiple data sets and use the cut rdb and the cut metastasized file we also use column one and in both files we have a header line and we keep only the entries that occur in both files and this gives us now for every unibroad accession of the significantly regulated proteins the abundances in every sample and next we will visualize them with the heatmap tool so if we don't give this a plot name we can again put in both files that are joined and we don't need a clustering and we scale by row and you can see that the tags always continue here sometimes it gets a bit hidden but once you click on the tag icon you can always see it and then you know which heatmap or which file belongs to which of the two conditions and you can already check on the numbers so we have here 13 lines in the rdb and we have 86 lines in the metastasized file and here's the heatmap and we can immediately see that for the upregulated in rdb proteins there's definitely more and higher intensities in the rdb samples than in the metastasized samples and probably it looks very similar here yes we can also clearly see the distinction that here are higher intensities in metastasized than in rdb as a last step we now want to find out which proteins are behind these identifiers we have here there are different ways how one could do it we will use the unibroad tool to retrieve the protein names we can again do it in parallel for both the metastasized and the rdb file and we select that we would like to retrieve our entries by using the unibroad accession and we obtain them as a faster file but because a faster file is not so easy to read for many proteins in a last step we will change the faster file into a tabular file with the faster to tabular tool we can again do the same procedure for both files and we split up our title into two columns and here we can look at one of these files so by using the unibroad accession we now receive the full faster entry which includes also here the protein name that's what we are now interested in and because this is hard to read with all the sequences in between we use this faster to tabular file and here we now have it nicely sorted so the first column is the accession and the second column starts with the protein name and in the third column we have the faster file and we can now have a look at these proteins so we are now in the file that contains the proteins that are upregulated in the metastasized squamous air carcinoma and there are a few proteins that were also found in the original study for example the x-ray repair cross complementing protein 6 and also the serum amyloid p-component and we have 12 proteins that are more abundant in the rdb squamous air carcinoma compared to metastasized so here we see the label again if it doesn't appear we just click on the label and here it's quite obvious that there are a lot of collagen here here here here and this is also typical for rdb itself that because of the missing collagen 7 other collagen are upregulated probably trying to compensate for the missing collagen 7 and one protein that we have here the collagen 14 there was also an immunofluorescence staining done in the original study which we can see here and we see that in rdb the intensity is way higher than in the metastasized cutaneous squamous air carcinoma so this was the training inside the training material there's more boxes that are important when you would like to run the training on your own files for example here how to set up the annotation file and the comparison matrix yeah and i hope that was helpful for you thank you for being here and i hope you enjoyed the training bye


Hey, this is Brett, and today I'm super excited to show you this brand new 2022 Ram 2500 Crew Cab Short Box Laramie Level B with the night edition on it. This truck has the 6.7 liter Cummins diesel engine. It puts out 370 horsepower, 850 foot-pounds of torque. Now this one's going to be capable of towing 16,660 pounds, and it has a 2,000 pound payload capacity. Now I found that information on Ram.com, there's a towing capacity chart right on Ram.com, so you can check that out. I have it linked in the description below. We're going to go all the way around on this truck. Granite crystal is the color, and we're just going to go over all the options. The Laramie Level B package includes the night edition, I'm going to show you the auto leveling rear suspension. This one has the UConnect 5 12-inch radio, and I'm going to show you that as well. We're just going to go over everything in this video. We shoot all of our videos in 4K, and if you like the way we do things here at Summit Auto, and the lower right-hand part of the screen is a subscribe button, gold subscribe button to our YouTube channel, click that, subscribe, and click the bell notifications. You'll get updates every time we do videos here at Summit Automotive. This one comes with the LED headlamps, running lights, and fog lights.  You also get the painted front and rear bumper with the night edition. You get the front bumper parking sensors. This one also has a safety group on it, which gives you adaptive cruise control, lane departure, and forward collision warning. You get the fender flares with the Laramie package. The 2500 Cummins and Ram logos are all blacked out, and that is part of the night edition as well as the 20-inch painted alloy rims. Comes with Firestone Transforce AT tires. These are LT28560R20s, and you can check out the tread pattern on them. This one has the add-on option of the gloss black step bars, and those look really good. Those are $895 option. In fact, we'll check out the original window sticker here. Feel free to pause this. I'm not going to go over everything, but most of it I will. So the 22 Ram 2500 Laramie night edition comes with the 6.7-liter Cummins diesel. It's paired up with the 6-speed automatic 68 RFE transmission. 373 gears on here, and then everything on your left is standard. And most of that stuff is going to be superseded by the Laramie level B equipment group, which we'll get to in just a second. Granite Crystal is $200. Leather trim bucket seats are $545. The customer-preferred package 2HH comes with the Safety Group B, which is $1765. Digital rear-view mirror lane-keep assist, full-speed collision warning plus adaptive cruise control and adaptive steering system. Protection Group, which gives you the transfer case skid plate shield, is $95. The fifth wheel and goose neck towing prep group is $545. The night edition is $2,695. You get the tow mirrors, the painted front and rear bumpers, the painted 20-inch rims, and the black interior accents. The Laramie level B group is $4,325. Gives you the ventilated front seats, the in-floor storage bins in the second row, rain-sensitive windshield wipers, remote tailgate release, heated second row seats, radio driver mirrors, pedals, memory, mirror mounted auxiliary reverse lamps, 17-speaker Harman Kardon premium sound, Sirius XM360L with six-month subscription, power-adjustable pedals with memory, so that's everything in the Laramie level B group. The anti-spin differential is $495, and the Cummins diesel is $9,495. Also gives you the tow hooks and the 220 amp alternator. Instrument panel mounted auxiliary switches are $145, and those are these guys right here, if you're wondering about that. Has the clearance lights, which are $95. The LED bed lighting, which is $165. The painted flat wheel-to-wheel side steps are $845, and then the automatic leveling rear air suspension is $1,705. You connect five nav with 12-inch touchscreen display is $1295, and then the spring bed liner, which is really nice, by the way, we'll check that out in just a minute, is $600. Now your total MSRP is $84,325. With the availability on these heavy-duty trucks right now, the prices are a little different than the MSRP. So you can check out what we're offering this truck for in the upper right-hand part of the screen and link to our website with the pricing and availability on this truck. Three-year 36 bumper-to-bumper, five-year 100,000-mile powertrain warranty. This is your greenhouse gas and smog ratings, which are pretty good. And the overall score is a four-star for safety ratings, four and five-stars pretty much up and down the board, except a roll over, which is three, but still a very safe vehicle. And I definitely would feel safe driving my family around in this truck. So we'll come around to the back here. You can see you get the LED tail lamps there, the blacked-out 4x4 RAM and Laramie logo, the painted rear bumper. This one has the full towing package, which includes a receiver hitch, four-pin and seven-pin wiring. You'll also notice that there's a hook up here, so that's for a trailer camera. So with the rear-view camera that comes on this truck, which is mounted up there just to the left of the lights, that's the rear-view mirror camera. You can have it, so you can hook up a trailer with a camera on it, and then that'll be functional through the trailer camera. So that's a pretty cool system, and I believe the wiring and everything is in the truck for that. In the bed you have the very nice Mopar spring bed liner, the fifth-wheel gooseneck prep package.  You get the cargo lights back here with the LED bed lighting, and side box tie-downs, bed rail covers, and you can see that those lights up on the cargo lid up as well. Tailgate's really light, easy to lift. Now the RAM air suspension, you can always tell which ones have the air suspension because they got the sticker in the window, obviously if somebody removes it, you can't tell. But it's a pretty good indicator that it has it. Now if you go underneath here on the driver's side, you can see this airbag right there. So that'll pump up the rear end to make the truck level if you have a really heavy load, and I'll show you how that works here. Now those also replace the coil springs that normally come on the 2500s. So this lever right here, it'll get pushed up, when the truck's got a heavy load, when that gets pushed up, it tells that airbag to pump up until it's back to its level state. So pretty cool technology on this truck for the towing capabilities. You can also lower the bed and everything, so that's really cool too. Lane mirrors, directional signals, they power fold in, they fold up like so for towing, and they do have LED side lights, we'll turn those on right at the end of the video. Inside the Laramie level B package gives you the black leather and suede interior. You get the Laramie stitched on the backrest, you get the gray, light gray piping, lumbar, power driver's seat, power passenger's seat, heated and cooled seats. You get the Berber floor mats, auto headlamps, power pedals, power windows, power locks, power mirrors, the suede inser, you get kind of the diamond plated finish to the inside of the door and memory driver's seat. Like I said, these mirrors power fold in and out. And then the other neat thing with these mirrors is you can control, you gotta press this button and then which mirror you wanna control, and then you can control that outside blind spot mirror, so that's pretty cool too. More handy on the passenger side where you'd have to get out and adjust it manually. We'll hop inside here, check out the Myles radio and everything, an instrument cluster that this truck has to offer. Get the digital speedometer, compass display, you can put time, Myles per gallon, Myles to empty, factory brake controller, settings and all that stuff in the corners, you can change all of that, only 12 miles on this truck at this moment. You get the heated leather wrap steering wheel, information center controls, Bluetooth controls on the left, audio controls on the back. On the right hand side you get the cruise controls, adaptive cruise controls, so it'll keep you a certain distance from the vehicle in front of you, and then you have the gear selector right there as well. That's part of the six speed automatic 68 RFE transmission, and now this is the UConnect 5, 12 inch radio, so this radio is run on Android, and it's supposed to be five times faster than the old UConnect 4 system, as far as just usability and switching between different screens and all that. I personally don't think it's five times faster, but that's what I have read, so that's what they say. Anyways, it's still quicker than the old UConnect 4 system, so you have the factory navigation system on there, you have your home screen, which will take you to your radio and the nav, and I think you can edit these, you can make this screen bigger, you can edit which screens are on there, you can do your shortcuts and all that, you can do your heated seats, cooled seats and all that stuff right here, obviously the car's not on, and when it is on, you can control all that, so you have heated and vented side there. You also have your phone you can hook up, it has Android Auto, Apple CarPlay, and what's nice about the UConnect 5 is you can do all that wirelessly now, so that's pretty sweet. You get your different vehicle options right here that you can do, you have your controls, the bed lowering like I was talking about, the rear view camera, and those rear view cameras now are HD on the RAM 2500, so they're crystal clear, and then you can zoom in on the receiver hitch to get hooked up to your boat camera, camper or trailer the first time every time, and then you have the off-road pages, which they started putting on the trucks, you got your steering angle, which that changes as I turn the wheel, you have your different accessory gauges and your pitch and roll, you also have latitude, longitude and altitude down here. This would be super handy if you're doing off-roading or even parking a camper in a campsite to make sure that you're on level ground and whatnot. So you have all your different apps here as well, and you can see all those different apps there. We could go through this radio for hours, but we're going to continue on here, lane departure warning, stability control, the Harman Kardon sound system, down here you have your push button four-wheel drive, factory brake controller, you have your alternate trailer height, so that's part of that rear airbag suspension system, you can have different trailers programmed in there, tow haul mode, factory exhaust brake, front to rear parking sensors, you have your six auxiliary up-fit switches, two USBs, two USB C's and an aux jack, the wireless cell phone charge pad, and then you do get 110 volt, I'm sorry, 115 volt 400 watt plug-in.  Up here you have your rear view mirror camera, so that's basically a video display of what's going on behind you. Now you can turn that off and just have a normal rear view mirror, and then you have a couple different options on here, so like I said, you could do the trailer if you had a trailer camera hooked up, but you can do the brightness, you also can do, you can adjust if it's looking up or down, so I guess depending on who's behind you or what you're watching for, you can change that. So really a cool system, I even have this technology in the NASCARs now, so that's kind of neat too. Home link buttons for your garage doors, security systems, and lane systems, assist and SOS buttons in the upper console, power sliding rear window, and then your map lights. We'll check out the back seats, and then we will turn on all the lights, start it up, do a final walk around on here. Back seats are heated on the outboard side as well, latch child safety system for any child car seats you may have, power sliding rear window with the built-in rear defrost. Notice you get the speakers and the ceiling part of that Harman Kardon sound system. There's also a subwoofer underneath that seat. You get storage under the seats, you also get a load floor, which is nice if you got muddy boots of tools, put them on there, you're not going to get your seats dirty, or if you got pets, put both sides down. They have a nice flat place, because you can see they've got the big hump here. There really wouldn't be a good place for a pet to sit comfortably with, except on the seats, of course, but then you're going to wreck your seats. Heated seat buttons, two USBs, two USB C's, and then a 115 volt 400 watt plug-in. You get that nice Berber floor map back here, and in-floor storage back here as well with the in-floor storage bins.  You do get child safety locks back here. All right, let's start it up. We'll do a final walk around here with all the lights on, and then you can hear just how quiet this Cummins diesel is. It's a little loud starting up, but once it's started it's actually a pretty quiet engine. We'll take a look under the hood, too. You can see I turn on the LED with the cargo lamp light. You turn on those LED bed lights, and you also turn on the LED side lights on those mirrors, and they are extremely bright, which is handy at night if you, I don't know, parking a camper, working on something next to the truck. See those LED tail lamps? You also get the LED license plate lights on there, and I would just personally like to thank you for checking out the video today. We do videos like this, like I said, each and every day here at Summit Auto of our new and used inventory. We probably have one of the largest catalogs of vehicle walkarounds on YouTube, so definitely subscribe to our channel, go explore. There'll be some links at the end of the video here to go check out more Ram vehicles and stuff like that. LED lights, super bright. The fog lights, the running lights, the headlights. You do get LED clearance lights up top there as well. Cummins diesel. Very, very smooth. Very quiet. There's your emissions sticker. Now I'm going to show one more thing. Everybody, even though I gave you the towing capacity and everything, people like seeing the VIN sticker here because it's got your gross vehicle weight rating and all that other information, so feel free to pause this, your VIN sticker, and then the tire and loading information sticker right there. So there you go. All right. Once again, thanks again for checking out the video. Really appreciate the views. And this truck is currently available for sale if it doesn't say sold in the title. And see more pictures of this truck or one of our 450 new and used cars, trucks, SCVs, minivans, ringlers, halftones, three-quarter tons, one-tons ename it. We got to go to that website right there, www.summonauto.com. Full pictures and descriptions of every single vehicle from two locations all at www.summonauto.com. If you'd like to check out more HD videos, you can go to www.youtube.com. Remember to like, subscribe, and share on this video and all the videos that you see there. Click the bell notifications. You'll get updates on the videos we do each and every day here at Summit Automotive. In fact, in a second, we'll see a link to subscribe to our YouTube channel in the upper left, a link to more Ram 2500 truck videos like this one, the upper right link to this vehicle on our website in the lower left, and a link to one of our latest YouTube videos in the lower right. Click those check us out and we are super excited to be offering the 2022 Ram 2500 Crew Cab Short Box, Laramie Level B Night Edition, and Granite Crystal Pearl Metall with the 6.7 liter Cummins Diesel. Thanks again for checking out the video. Really appreciate it. Remember to like, subscribe, and share.


Do you ever wonder what happens when the police leave? Crime scene cleaners are private companies that handle the cleanup after the police are gone. Spalling Decon is one of the nation's largest cleanup companies handling the aftermath of homicides, suicides, decompositions, hoarding, and much more. These are our stories. Alright, Mario, in Tampa, Florida, what are we here for? We're here today for a level five hoard. We got some tenants, one's been baker-acted, and the sister has told us she needs us to come inside, clean out all the house, get everything situated so that it can be back livable or if she just wants to save the house and sell it. So we're here to get everything cleaned up, everything trashed, cleaned out. We do have a couple of items that she wants to save, which obviously we're going to do. We're going to put that to the side, document, save it, let the homeowner know all about the process of what's going on today. We'll be here roughly a week trying to get this level five hoard cleaned up, situated so that it's back livable, and we're going to get started. We're going to get to it because this is a big job, big process, but we've got a lot of hands on deck this week, so if we're here, let's get to it. Gas can. We don't need more of those. You can tell you've been on it. Oh, it's like you're not going to mess with it. Oh, Kukarachas! Anything good in here, Sheldon? Coastals. I'm going to ask you what's your name for the camera? Dylan. Dylan? Yes, sir. I heard Sheldon saying that you were a trainee for a franchise. Yeah, I'll be with Christian out in the winter garden area. Okay, so you've probably seen some pretty bad stuff too, right? Well, this is my second week doing it out here with these guys. When I come from a moving background, I've seen stuff like this before. Not as bad, but we've dealt with the smell sometimes, and all these boxes and stuff like that. And how does this hoard compare to all the other stuff you've done?  So far, yeah, this is the worst one just because of the feces inside, but it's not.  So, I mean, it should be pretty easy once we get in there. It would be pretty quick. It's all boxes, okay? Oh, there's something to jump out on me. See how I can jump back? Okay, nothing on that. It's all trash. Actually, it should be cleaned up. Yeah, that's cleaned in the house. Yeah. Take that. I'd live in here, come to the house, play. So, is that all trash, Sheldon? Huh? Everything in here, like these boxes and stuff. Yeah, we were told by the sister that everything in here goes. So, what are you doing right now, AJ? Basically, right now, we're cleaning up the shed. Basically, anything that we know of, like household stuff that says a counter on the office stuff, little files we're going to keep, anything else, just tossing it up, that's really it. Yeah, and I see like these two areas is like nowhere near as bad as the house. Yeah, man, honestly, we're going to get through, like I said, we're already basically done with this last box. We jump on that, that's going to be quick, but the house is definitely going to take more time than this. Yeah, and like your personal experience, is that the worst you've ever seen? Like in a horror story? Far species goals, and you're in yes, but the last what I just did with Mario Shelby and Tyrone, it was way worse. We found needles from diabetic. I found a live turtle. Okay. But yeah, this definitely is one of the worst ones I've seen so far. Oh, boy. Yes, sir. That's how you get it in right here, man. So, Mario. Yes, sir. So the dump's full right now, and you have to make a trip over there. Make a trip to the dump, come back. Got to do it again. Yeah. Go to the dump.  And then we just, what happened? Been right there. It wasn't spraying it up. Oh, that's cool. Oh, yeah. Full thing. It's only been like an hour of work, too, and it's already full. Yeah. Hold on, we don't get it done. We don't get it there. We don't get it done. I don't see no more. That's live. Every good. I was going to like live back. What you can hear is, it's tough to like to pull it. Discuss. Fresh milk. It's chunky. All right, Shelby, end of day one. There we go. All right. So we were able to get everything out of the shed in the back of the house. We got the garage cleaned up completely. The only thing left in there is anything that we think might be what they want. Either be paperwork or something. We wanted to clear out the back porch and the kitchen got cleared out, but then we put all the big stuff in there and tomorrow when we get the dumpsters, we're going to just pull all that out or straight into the dumpster, then kitchen will be done. We got just about all the living room cleaned out also. So all in all, we got more done today than we were expecting. So hopefully tomorrow we can get started on the bedrooms and the bathroom. We got more day two, so that's what needs to be done today. So we got a lot accomplished yesterday in all reality. We got to finish getting the kitchen, a couple of aquariums inside the living room. Then we're just going to work our way to the back. There's one room in the house. It's actually not that bad. But then the other one, it's just filled with feces. But I mean, it is what it is.  We get everything done, quick, fast, in a hurry. And we're just going to get to it as we do always. So like I always say, let's get to it. Let's get it.  We're sleeping. You're just doing a different type of work. Yeah. This is why I take pictures of everything, because I definitely can't use this anymore. It's like pristine. Bro, bro. We got a dog collection monopoly.  What wolf hat?  Yeah, this is this game. That's what we're going to get through it.  I already found the title to the ladies car in here. If not, I'm just throwing away stuff. I can't do that. Are you coming this way? Go away. Get all of it and just come right to the floor. Come on, bro.  Everybody's trying to make it seem like this is harder than what it is. But in all reality, this is a cake walk. Just like when people on the news, everybody is afraid of the unknown. But once you get there, you see it, you put your eyes on it. It's like, you made this seem like a big deal for what? You got this. Is it crazy to think that you guys might be done by like today? I know we'll be done tomorrow for sure. When we first died here, we kind of knew that it wasn't going to take four days. I mean it was bad, but it wasn't like, it's such a small house that we knew it was going to be done in three days. Yeah, because like after the car was traveling, what else is really much to do with stuff and getting all the drugs? Just kind of cleaning up. Obviously, we've got to scrub the floor and clean that with some chemicals and wipe down the walls and stuff in the dust. That's pretty much it. We'll be out of here. Come up.  I've got to get a pair of pliers.  If anything, I wouldn't be surprised if she had some kind of vet for me. Yeah. Are there any loose needles or have they all been here? No, they're all in packaging. Oh. I've liked you've done this before. You got the entire one? Yeah. Might as well. Yeah, end of day three.  At least we did. We're in here to get this job done. We're in the business of making homeowners and clients happy. And the faster we get our job done, the better off it makes us as a company. So we get in here, we grind it out, make sure they're all happy. Everybody satisfied with the job we do. Let them come through, walk through. They have any concerns. They address them to us. Any final that they want us to do. We get in there. We're in there like swimwear, buddy. Hell yeah. Oh, yeah. Come back day three. Come back three, day three. Grind it out some more.  On to the next one. Okay, Mario. Day three. How's it going so far? Majority of the house is done. We got our final clean. Got some little bit of stuff in the bathroom and in the hallways. And the refrigerator that needs to be cleaned out. Other than that, it's just going to be a day of basic just cleaning. Making sure that we get everything done, clean and up to standards so that the client can come back inside. And if they want to sell the house, they sell it. If not, they just get everything redone and put back inside so they can move back in. That's the game plan for today. Get everything done. Make everything nice and neat. And hopefully it'll be the last day for us. It won't be a day for it. All right, let's do it. Let's do it. Hey, hey, hey. Hey, hey. This one. Is that the fridge, right? The freezer isn't too bad, but everything's basically spoiled so that means it's not working right. Are you just sort of spraying it down? Yeah, I'm just spraying it, wiping it down, basically pulling up all the dirt and grime that I put it off of. It's going to come right off as you see. Yeah, pretty easy. How many more? Not again, not again, no more. Yeah, this one. Oh my God, a lot. That is the egg and the yolk. That's cool. It looks like grass. You don't want anything? Huh? You don't like it? Yeah, it's on the floor. The house is basically on stilts. They have a crawl space up underneath the house and all the wood and stuff, so it's like you have your trusses running straight across the floor and then they just build the flowing directly across the truss. And then whatever floor they put on top of it, they either tile, vinyl, laminate, carpet, padding, tech strips, padding, carpet, right off top of the free flow pool. Here, how long that is? If you're going to step through this too hard, free flow pool straight through. We'll see the waters back on. We just turned it on because we need water to help clean everything. Man, they got to replace that whole pipe. Yeah, because if that part's broken, there's probably more of it that's broken. They got to replace the whole pipe. They're inside in the bathroom now. Yeah, we're going to do the kitchen floor and then the bathroom toilet.  Oh yeah. I have no choice but to. Homeowners, they say the only one is the one. Because of all the nastiness that's on it. Do they see it? Oh yeah. Oh yeah? Oh yeah. What's? Because I got to get, because I scrubbed it. I put Part A. Let's see. Shockwave. Scrub it. You can still see all the foam over there. I want to put water in it to rinse to see how much came up. But if the shower doesn't drain, that's coming up. All right, Shelby. End of day three.  How did it go? It actually went really well. We got done at least a day before we were told. We were told four days you have to clean. We got done in three. So that's always a plus when we can get done faster than what the clients were expecting. But we managed to get everything up and out. Carpet, flooring, everything out. We did clean the floors as best as we could. Fortunately, some of it is not coming up no matter what we do. And most of the flooring is going to have to be done in this house anyways. But kitchen looked a heck of a lot better. Bathroom. Eh. Not really. But that's just messed up anyways. But overall, we're all done. We got the job done. Hopefully the clients are happy with what we did. Guess we'll find out tomorrow. Good work. For more information, visit any of our locations.


Hey guys, it's Nate, aka The Foot Account. Welcome back to the channel, little double upload situation today. All right, we're talking FIFA 21 because there's a lot of stuff that happened today with the EA Play and the EA Live event about FIFA 21, kind of just building the hype for the next game of FIFA. There's a lot of information that came out today as well that we can actually learn from, read into, and kind of draw some conclusions from already. Some surprising ones as well, a actual later release date for FIFA 21, which is going to be interesting. And I think a lot of this stuff from a hardcore FIFA user base is going to be something that we find a bit underwhelming. But I got to say this for starters, I got to say this for starters with these early, you know, when they just tease the game right off the bat, when they tell us like, hey, this game, this is FIFA 21, this is like the trailer, watch this, like we never see and we never get a lot of information right away. They always tell us, hey, this is what we're going to do. And then down the line a little bit later, they give us the actual like info that we really, really, really want to know. So right now, this is just very surface level info from EA. But there's still some stuff that we can learn from this. And that's what I want to talk about in today's video, especially FIFA 21 being delayed and being later than it was in previous years. But one thing we can confirm already, we know the ones to watch car design, that's a pretty sick car design, starting off FIFA 21, with that car designs actually pretty cool. Because this is the preorder, you get the OTW card involved with there, they're already kind of promoing the preorder and stuff like that, you're going to see this for a while, you're going to have a lot of time to preorder the game, don't worry about doing it now. But a lot of the next gen console stuff is what they're pushing you can see in this little infomercial here, feel next level in FIFA 21 with a PS5 and Xbox Series X with new technology that takes the world game, the world's game from visual to visceral. And I'm not actually going to watch the trailer, you can watch that for yourself if you want to. But I'm not super impressed with it to be honest with you. It's just, you know, a one of those trailers that comes out way, way, way too early before the game is actually going to come out. So the biggest thing I want to talk about today is the release date, right? Because EA gave us a lot of information in this FAQ section. And I want to talk about that as well as the dual entitlement with the PS4 to PS5, Xbox One to Xbox Series X. I want to talk about that with you guys as well. But this is the big thing today, right? When is FIFA 21 coming out in the PlayStation 5 and Xbox Series X? We'll have for information to share about this later. Okay, whatever. That kind of just depends upon when the PlayStation 5 and the Series X come out themselves. But this is the biggest information. When is FIFA 21 coming out on PS4, Xbox One, and PC? FIFA 21 will launch worldwide on October 9th. October 9th is the release date for FIFA 21, which is actually two weeks later than the original start for FIFA. Usually FIFA starts like the second to last week or last week of September. So this is about two weeks later. And there's a lot of stuff that is still hanging in the balance earlier this year. EA told us that if FIFA was not going to be delayed, and I mean technically two weeks, that's not that big of an issue in my opinion. I'm not too like been out of shape about this being delayed two weeks. And of course, EA has to get their football game out. The thing that I'm worried about is just the stuff that we know about this game already. And just that the engine is going to be the same, the frostbite engine is going to be the same and more stuff that we'll talk about later. But this is the biggest news that FIFA 21 is going to be delayed a couple of weeks. If you preorder, you'll get it three days early. And it looks like they're doing the EA access and Origin access again, or the one week early play first trials, which you will usually get like what is it like a 10 hour trial access. So we'll be talking about that sort of stuff. And it's going to be a little bit later this year, a couple weeks later than normal. So that is just something that's kind of interesting. They also promote the upcoming feature news. And this is the stuff that we really need to be worried about because this is the stuff that matters. So basically, they said coming in August, and this is what they did last year, they're going to be giving us some dates where they're going to go like it's going to be a couple times a week. And then they'll spread it out. They're going to specifically focus in on gameplay, career mode, bolt up pro clubs, and then finally FIFA 21 Ultimate Team is what they'll focus on in different like segments, where they'll do shows, where they'll do presentations and release videos and stuff like that. So that's what we have looking to look forward to in the month of August is this information coming on FIFA 21. So it's kind of like they tease us with the FIFA 21 stuff right now, they show us a trailer, they show us some graphics, maybe give us some info, especially this year with the new consoles, give us some info on that. And then we're kind of left in the dark, honestly, we're really left in the dark for like two months from now until August, we're really not going to know much unless there's some other games conferences that are going to actually happen. We probably won't hear much or know much else about FIFA 21 until then. But I do want to talk about the dual entitlement stuff because this is also pretty interesting coming from EA sports. And this is I think similar to what they did from the Xbox 360 to the Xbox one ps3 to ps4. And likewise, but they're really, really, really pushing the they're really pushing the image here on the new innovations unlocked by the new consoles blazing fast load times, deferred lighting and rendering enhanced animation technology off the ball of humanization. You know, feeling next level every time you step on the pitch. Yeah, we'll just leave that there. And because yeah, whatever. Anyways, for FIFA 21, this is the most important information. We are partnering with Sony and Microsoft to offer you a dual entitlement meaning you won't need to buy FIFA 21 twice to upgrade your copy of the game from ps4 to ps5. But that is not really new information. We figured that that was going to be what they would do with this game. And because that's what they did last time around. And that's just kind of like what you do, because you know, EA knows this, we all know this. EA sports does not make a majority of their money on FIFA ultimate team or FIFA in general, from game sales, they make most of their money from in game transactions, micro transactions, aka FIFA points, and however much money they make from like the volta shop, which is probably not much, but it's FIFA points inside of ultimate team is what they make. So basically, the dual entitlement is if you buy the game on ps4, you will be able to upgrade it on or do download it on your ps5 or Xbox One to Series X at no extra cost, which is cool. Thank you, EA. We like that. How do I make sure I benefit from this? Basically, if you purchase the game, you'll be able to upgrade since you have the corresponding new generation code and no cost on your console until the launch of FIFA 22, you'll be able to download it. I'm guessing that is like linked to your account when you like sign into your your PSN or through your Xbox account, you kind of have that your email and your password and you have games that you buy with that account. So if you buy FIFA on the PlayStation 4, when the PS5 comes out, you load up, you sign into your account and FIFA should show like in the download section. Basically, I think that's how it works to download. I think that's how it should go. You shouldn't have to get a code from EA. It should just be there and ready to go once you get that new console. And this is also a pretty interesting question. Do I have to purchase FIFA 21 digitally to benefit from dual entitlement? So this states here, the physical copy of FIFA 21 on PS4 also includes your entitlement to download and play the PS5 version of the game. Same thing with the Xbox to the X1 to the X. So you'll be able to upgrade via dual entitlement if you purchase a next gen console with a disk drive. If you intend to buy a diskless next console next generation console, you will need to purchase FIFA 21 digitally to take advantage of dual entitlement offer as the physical disks cannot currently be used to upgrade to diskless consoles. So if you were going to buy the physical disk, you're going to go to like a midnight release for FIFA 21 and buy the actual hard copy of the game just so that you can get the satisfaction of tearing that disk up at a later point during the year when FIFA ticks you off. I know some of you guys do that. You can't really do that this year unless you're going to buy the game again digitally if you upgrade to the new console. So that is something to take note of. If for somebody who buys the game on a disk, you're going to make sure that if you buy a next gen console, which I don't even know if these consoles have like the PS5 or the Xbox Series 1 X, I'm not following those consoles a bunch. I don't know if they have disk drives or not, or if they have multiple versions that do and don't have it. But that is something you have to take in consideration if you are somebody who buys a disk for FIFA. And then yes, all the progress that you make or acquire will transfer as well as Volta seasons. And no, it just Volta as well as progression and Volta will transfer from PlayStation 4 to PlayStation 5. So this is a big thing as well.  If you digitally download the game or if you get a disk and you can put that disk into the PS5, then you sign in with your account, you'll be able to download everything and it will just basically everything is tied to your account, right? Everything is just tied to your email that you have created your PSN ID with or your Xbox ID. It's all created and tied to that. So you will be able to download and have all your progress saved and move forward. So it looks like Ultimate Team and Volta will go forward. But online seasons co-op career mode pro clubs will be specific to the console you are playing on and won't transfer between consoles. So if you're somebody who is going to be getting the new Xbox or the new PlayStation and you grind a lot of career mode online seasons co-op or pro clubs, I'm really interested to see what pro clubs is like.  But pro clubs could look really interesting this year and you might have trouble like matchmaking. You know, if you're on a PlayStation 4 or PS5 in kind of those early stages, until later in the year, when more and more people start to acquire that PS5, it could get kind of interesting with the amount of people that start pro clubs on PS4 that will still be playing on PS4 and it will be playing on like PS5 or Xbox One to Xbox Series X, just a thought there. And then yes, this again talks about the digital copies and the not digital copies. And then also, once I upgrade, can I go back? So for whatever reason, you yes, you could go back if you want to. And you cannot go from Xbox One to PlayStation 5, you cannot go from Xbox or PlayStation to Xbox. It does not work like that. It only goes from platform to platform. And just a lot of other questions here about the disks and all the questions about that and whatever. So I want to click on this, the field next level. Actually, no, I don't. I don't want to look at that. No, thanks. That's just the same stuff that we looked at before. But basically, that is what I want to cover today in the video, as I want to talk about the dual entitlement. And I wanted to talk to you guys about some of the stuff that we know about FIFA. And we really just don't know a lot yet. We know they're going to be using the Frostbite engine again, based on some of the stuff that we saw in some of those videos. And basically, that's the same engine that we have on FIFA already. But that is something that is really not new. That is something that is really not new. It's the same engine that we've had in FIFA since FIFA 17. And basically, to sum it up, FIFA's coming out two weeks later than normal. There's no cross platform play that didn't talk about any cross platform between the Xbox and the PlayStation playing together. The same engine, the same servers, which they are improving. And the actual in-game content, they also said this during the live stream, the in-game content on FIFA this year will depend on the COVID situation. So stuff that's going on with COVID-19, the coronavirus, the in-game content and the promos with Team of the Weeks and other events like the UCL and stuff like that. We might see some interesting promos at the start of the year that are based off of maybe non-real-life events, like they might have to just start boosting some cards to try to make some money in their game. You know, they're going to try to make some money because it's foot with the microtransactions. So like an ultimate screen promo is something that they could still run earlier on in foot. They could still run once to watch. But it's going to be interesting, like with Team of the Knockout stage, if whatever the Champions League situation is at, we might not be starting the new season of Champions League yet or the new tournament at that time. Who knows, right? This is something that's just kind of evolving and happening as we continue to go through the COVID process. So a lot of question marks around the content. But honestly, not a lot of info that we learned today from the FIFA events. Seriously, not a lot of info. But how much can you expect, right? How much do you really expect from an event that is this far away from the launch? We're really going to be left in the dark now and not hear much for the next two months. EA did talk about some other really cool things they're doing with like EA Skate. I know a lot of people are pumped about that, Apex Legends, and there are other games. There was some hype around those. But at least for FIFA, we still are going to be waiting until that August timeframe when we see the more in-depth stuff and we'll be covering that stuff appropriately as it does come out. But I wanted to give you guys kind of an overview of what happened today in terms of the EA event, if you didn't watch it, which I actually didn't. I just went on and read all the pages and looked at some clips and stuff of what happened. So that's just kind of the info that we got today. If you did enjoy it, smash the thumbs up on it, comment down below if you have any questions, and subscribe to the channel if you're new. It's been Nate, The Foot Accountant. Catch you guys later. Peace out.