Good morning. I have general questions, his first item of business. He may not have been lodged, but I will see him Yuan for questions to ask Stuart McMillan. First Minister, I thank you very much for joining us today to ask the Scottish Government what its response is to the reported reduction in the number of childminders working in Scotland. Minister Youth of Scotland, the Scottish Government recognises that childminders are an important element of the Scottish childcare sector, offering high-quality, unique and flexible experiences of childcare for families That's why we're supporting an innovative child-minded recruitment pilot being led by the Scottish Child Minding Association and Partners, aiming to recruit and train more than 100 new child-minders in remote and rural areas, with the recruitment of these additional child-minders up to 900 childcare places may be created. We have also provided targeted financial support to child-minders during the pandemic, including issuing over £3,000 grants worth £950 through the Omicron impact funds. We will continue to work with our partners to increase the number of child-minders in Scotland through the implementation of our commitment to child-minding action plan published in 2021. I thank the minister for that reply, and I warmly welcome that the Scottish Council in 40 hours policy is saving families on average £5,000 per child a year, but it's also crucial that free early learning of childcare is flexible to meet the needs of parents, and that's why the loss of 1,671 child-minding businesses in Scotland in the last five years is extremely worrying. Can the minister therefore outline what further steps the Scottish Government is taking to increase the AOC workforce, as we will need private and voluntary childcare settings, including child-minders, to continue to expand the free fund of childcare for children and families? We want families to be able to access the flexible, supportive and high quality childcare that child-minders can provide, including as part of the funded early learning and childcare entitlement. It was encouraging that the Scottish Child Minding Association's 2021 audit showed an annual increase in the number of child-minders delivering funded ELC. We are working with the sector to explore how to encourage more child-minders to offer ELC, including identifying opportunities for reducing burdens on child-minders that may prevent them from offering ELC. We are also working with the sector to identify the reasons for the decline in child-minders, including ensuring that the interests of child-mining sectors are represented on national forums, such as the childcare sector working group and the new national provider forum. That helps us to identify where practical support can be provided across the sector. I thank the minister for that question. Can she go further on explaining how the outreach to the child-mining sector and also the private providers within the ELC is occurring? Will she confirm that the correct weight will be given to that evidence, because there is a crisis in early years and it is getting worse? As we move into this winter period, particularly with the cost of living that is hitting businesses, we could see a massive drop in places very quickly. I am very sorry to the member if I have misunderstood his question in terms of supporting ELC and child-mining businesses across the piece. We are doing that through our national forums by ensuring that there is representation of their representative bodies on our national forums to look at the training and development that we need to ensure that there is a highly skilled workforce right across the sector and that it is a workforce that we are able to recruit to and also to retain. If I have misunderstood his question, I am more than happy to write to him. Funded ELC entitlement can be used at a child-minders, nursery or play group, but parental choice is limited by the availability in their area. The value of child-mining for children's development should not be ignored. It has low adult to child ratios and enables children for different ages to learn and play together. What further support can the Scottish Government offer to ensure that there is adequate child-mining provision across the country, including in Shetland, where there are now only three child-minders? I thank Beatrice Wishart for her question. I am very aware of the support that we are providing to remote and rural communities in terms of accessing childcare. We will continue to work with partners and local authorities to understand the needs of our remote and rural and island communities. Those needs will be taken into account as we develop our strategic framework for Scotland's childcare profession, which will be exploring a range of issues under themes with partners, including recruitment and retention of ELC professionals across Scotland. The member may also be interested to know that I will be visiting ELC and child-mining sectors in our remote and rural communities before Christmas to engage with ELC professionals directly to hear from them. To ask the Scottish Government what assessment it has made of whether inflation and any possible reductions to public sector spending by the UK Government will impact on prospective capital projects in Midlothian South, Tweeddale and Lauderdale. The level of inflation seen over the last few months has been unprecedented in modern times. That combined with increases in the delivery times from materials due to the combined effects of Covid, Brexit and the illegal war in Ukraine is placing significant pressures on budgets and the delivery of infrastructure projects across the country, including those in Midlothian South, Tweedbank and Lauderdale. That will be reflected within our latest six-monthly reporting of capital projects to be published in the coming weeks. Any reduction to our capital budget by the UK Government would exacerbate that situation further. I therefore urge the UK Government to protect and enhance Scotland's capital allocation in the upcoming autumn statement to allow our capital programmes to continue at the required pace. Within Midlothian South, Tweeddale and Lauderdale, two projects that spring to mind are the proposed extension of the border railway and the redesign and construction of the Shereiffall roundabout. I note that the minister is going to report. Can the minister advise of any specific impact on those as a result of raging inflation falling from the mismanagement of the UK economy by the Conservatives? Despite cuts by the UK Government to Scotland's capital allocation and uncertainty on future allocations, the Scottish Government remains committed to investing in road improvements like the great separation of Shereiffall roundabout. Transport Scotland continues to progress the proposed scheme through the statutory process. The public inquiry is now scheduled to take place on 30 January 2023 for a period of two weeks. The same is true of our commitment to decarbonise our railway, and decarbonisation of the existing border railway is estimated to commence construction in 2023. To ask the Scottish Government what action it is taking to ensure that all fire and rescue officers have the appropriate decontamination facilities available to them. The safety and wellbeing of all fire and rescue officers is of utmost importance to the Scottish Government. This year, we increased funding to the Scottish Fire and Rescue Service by £9.5 million. Decisions on the allocations of its £352.7 million budget is a matter for the SFRS board and chief officer. I am aware that the SFRS has been engaged with the FBU on work to, with Lancaster University for a number of years, and its well-established contamination working group has taken actions across all aspects of operations to reduce exposure to harmful contaminants. That includes investment in new fire appliances and fire station facilities. I take this opportunity to welcome the new minister to her post. Last week, Professor Anasett from the University of Lancashire presented the shocking results to MSPs of our research into the impact of contaminants and firefighters, but showing that UK firefighters are four times more likely to get cancer during their lifetime than the general population. The World Health Organization classifies firefighting as a carcinogenic occupation. Canada, the USA and Poland have presumptive legislation that tracks links between the workplace and exposure to cancer. Will the minister be prepared to discuss with me the possibility of presumptive legislation that we could pursue? I am aware of the work that has been carried out by Professor Steg. It is a value contribution to building the knowledge and understanding of contaminants, which could be harmful to firefighters. I am absolutely happy to meet the member to discuss this further. I know that the Scottish Fire and Rescue Service has implemented enhanced cancer-focused screenings, questions and discussions during routine medical assessments, and the health and wellbeing department continues to provide a service for post-diagnosis support and relation to employees with cancer. I am very happy to meet the member to discuss this further. I also welcome the new minister to her role. SNP budget cuts could lead to one in four firefighters being axed and one in four fire engines taken off the road. Over half a billion pounds is needed just to bring buildings up to scratch, with 14 stations in a dangerous condition. In light of those realities, is it not the case that firefighters will be sceptical of whatever decontamination commitments they might hear from the new minister today? As the member will know, any negotiations for pay are done at a UK level. As it stands just now, our budget for the fire service has increased year upon year, and we are currently not engaged in budget negotiations as yet. To ask the Scottish Government whether it will provide an update on progress with its commitment to challenge men's demand for prostitution as part of the equally safe strategy. We are taking this work forward through a framework for Scotland to challenge men's demand for prostitution and support those with experience of prostitution. To underpin the framework, we have worked with an expert group of stakeholders to develop fundamental principles that will ensure that equality, human rights and safety are at its heart. Work is progressing well and, once finalised, the principles will be adopted and published. In designing the framework, we will reflect the key aims of the equally safe strategy and a vision for justice in Scotland, including how best to effect delivery. I thank the new minister for that response, and all SNP Scottish Government administrations have clearly stated that sexual exploitation is a form of violence against women and girls, and that this includes pornography, strip dancing and prostitution. This exploitation can stem from power inequalities, poverty, coercion such as threatening the lives of relatives, abusive relationships that become pimping and sexual trafficking. Whether that be domestic or industrial or international, significant work has already been done through the equally safe strategy. Can the minister confirm whether she will meet with the A model for Scotland campaign before the close of Parliament for recess to hear from the voices of trafficking survivors of how that exploitation can be effectively tackled? I would first like to take this opportunity to thank the model for Scotland campaign group for their work in raising awareness of this key issue. I am aware that they have representation on the reference group that has supported the development of the fundamental principles, which, once finalised, will underpin our future framework for Scotland on challenging men's demand for prostitution and supporting those who are experiencing it. I am committed to continuing to work across Government, the Scottish Parliament and with stakeholders as our collective reports to tackling prostitution further develops, contributing to our aim to be a society that treats all with kindness, dignity and compassion. This will, of course, include continuing to engage with the model for Scotland campaign, and I look forward to continuing this work with them, and I will meet with them and those with lived experience as soon as I can. Can I also welcome the minister to her new role? The minister may be aware that earlier this year the city of Edinburgh council decided to put in place a nil cap as part of the sexual entertainment licence scheme. Since this decision was taken, the council has faced challenges including in court on its policy. Given that stripping is classified by the Scottish Government under their equally safe policy as violence against women, what support is the Government providing to councils who have taken the decision to put in place a nil cap as part of the licence scheme, but are now being challenged for doing so? This Government provided the new powers to local authorities to specifically make decisions about sexual entertainment venues within their areas just recently, as you have outlined. I am committed to making sure that we can take this legislation forward and support local authorities on the way. I will be happy to meet with local authorities to discuss that matter. Ruth Maguire Thank you, Presiding Officer. In September, Thai and Chinese women who suffered a horrendous ordeal prostituted in brothels across Glasgow saw justice as their traffickers were convicted in the High Court. I am sure that the minister will join me in commending the bravery of the women testifying against their abusers, the caring professionalism of those who supported them to do that and welcomed the convictions. Would she further agree with me that to end the violence of prostitution we must end the male demand that fuels this cruel trade? I join Ruth Maguire in commending the bravery of any victim of sexual exploitation in coming forward with their experiences. I know how hard that is. I commend the work across the public sector and the third sector to support them. Any form of sexual exploitation is completely unacceptable. I am equally of the view that prostitution cannot be considered in isolation. The developing framework to challenge men's demand for prostitution and support those with experience of prostitution will have direct relevance to tackling wider forms of commercial sexual exploitation, including human trafficking. To ask the Scottish Government whether it will provide an update on the uptake of hydrogen innovation scheme by private companies since its launch in June 2022. The application window for the hydrogen innovation scheme was launched to support innovation in renewable hydrogen production storage and distribution closed on the 31st October. The scheme received a high level of interest from private companies, as well as academic institutions, with over 70 applications received in total. Applications to the fund are currently being processed and successful projects will be announced in the new year. Rory McNair. I thank the minister for that answer. Residents in the constituency of Clydebank and Maguire have approached me with concerns over the application that has been submitted by PEO to build a plastic to hydrogen facility and hydrogen vehicle refailing station in Clydebank. The development is proposed to include a thermal conversion plan that will utilise in advance a thermal treatment process involving gasification to convert waste plastic into hydrogen electricity and potentially heat. Many of the concerns raised have been about potential hazards and unknown level or pollution that this may cause. I am on the side of my constituents who have also feel that have not been consulted on this proposal. Does the minister agree with me that the views of my constituents are of paramount importance when considering this proposal? As I outlined in our draft hydrogen action plan published last year, our £100 million hydrogen investment programme is targeted at supporting renewable hydrogen production projects only. For the purposes of our hydrogen innovation scheme, we have defined this as hydrogen produced using renewable energy and that is zero carbon at the point of production. Therefore, all applications to the hydrogen innovation scheme will be assessed against their potential environmental, societal and economic impacts. That may include impacts on areas such as carbon emissions reduction, jobs or skills creation, export potential contribution to us, achieving a just transition and the development of the hydrogen economy in Scotland. To ask the Scottish Government what its position is on whether fatal accident inquiries are fit for purpose. We have every confidence in the system that is in place for fatal accident inquiries and we keep those matters under review in consultation with the Crown Office and continually consider and evaluate whether there are ways in which the system can be improved further. The Crown Office has significantly reformed its processes to reduce the time taken to investigate deaths and to bring FAIs to court more quickly. Those reforms have already resulted in reductions in the duration of death investigations and it is expected that they will continue to do so. Parliament considered and modernised the law on FAIs in 2016 and there are currently no plans to revisit the legislation. Over a year ago, when I asked the question around the entirely avoidable death in custody of our marshal of the Cabinet Secretary for Justice, he told me improvements have already been made, further improvements are being made and we will continue to improve the system. Can the Minister tell us what improvements have been made in the past year, what confidence can we have that further improvements are being made and what exactly is being done to prevent the deaths of those in custody? I have deep sympathy for the family in this tragic case. However, this is a matter for the Lord Advocate. I would say that there is separate work being carried out, as the member has said, in relation to deaths in custody that are being taken forward. Each death investigation over two years old and every death in custody is carefully managed through the now well-established case management panel process. In addition to the new Covid death investigation team, COPS has recently set up a specialist custody death unit to investigate deaths in custody. The Scottish fatalities investigation unit has significantly modernised its processes to reduce the time taken to investigate all deaths and to bring FAIs to court more quickly, and a similar project has commenced in relation to the health and safety investigation unit. The views of a family are always taken into consideration and the count when deciding whether or not a discussionary FAI should be held. Jamie Greene Thank you, Presiding Officer. The leading cause of death in custody is, sadly, suicide. I'm particularly concerned about young people in custody. We know that in Cônton Vale we have one mental health nurse per 25 residents. In Polmont it's one, and I believe, 81 residents. The disparity is quite stark. We're simply not supporting these young people enough and the suicide rate is far too high, Minister. What are you going to do about it? The Minister for Public Health and Sport I would agree that any death in custody needs to be taken seriously. I think that we have to pay cognisance to the fact that mental health issues whilst in custody are very important, and I will undertake to do everything that I can to ensure that we progress on this matter. Thank you. That concludes general questions. Before we move on to First Minister's questions, I invite members to join me in welcoming to the gallery the Honourable Anthony Rota MP, Speaker of the House of Commons of Canada.


Hey what's up guys welcome to another video my name is Dylan and I'm a cycling coach at CTS and today we're going to be talking about how to lose weight for cycling the right way.  This can be hard to maintain though which is why most people who go on diets fail and regain the weight back. The strategy I'll be going over here has a lot of science to back up its long-term success and is one of the healthiest ways to go about losing weight. On top of all this the science shows it can help boost your recovery after a hard workout. I'm talking about understanding the concept of calorie density and utilizing it in your own diet. I'll explain that concept go into a little bit of science and then get into some practical tips that you can use to implement this strategy into your own life so be sure to stick around for that. If you're new to this channel I make weekly training topic videos going over tips and tricks that I've learned in my 12 years of racing and training experience that have gotten me to the top of the ultra endurance mountain bike game in the US and as a cycling coach at CTS. I also go into the science and your training questions so if you want to learn how to get faster or just more about sports science in general be sure to subscribe and hit the notification bell so you don't miss anything. If you have a training question or a suggestion for a future video be sure to leave it in the comments section down below. I do my best to answer all training questions in the comments section but the volume of comments has been really high recently so I apologize beforehand if I don't get to yours. It's that time of year when race season is fast approaching and you've gotten in a pretty good base and are doing some good workouts but you step on the scale and realize that that saddle I bought that's 20 grams lighter isn't going to make a whole lot of difference when I'm toting around a whole old steel road bikes worth of extra body weight or maybe you're not an overweight weight weenie and you're just overweight and most of the reason you ride your bike is to get healthier and lose weight. Either way what I'm about to go over applies to you. Now you may be sitting there thinking to yourself what is this skinny 20-something who probably has the metabolism of a racehorse going to tell me about weight loss. I looked like him when I was his age and I ate whatever I want. I understand but what I'm about to share is demonstrated in the scientific literature not just my own experience. Let's go ahead and jump into some science by starting off with why losing weight can be such a challenge even as an athlete who trains for hours a week. For starters it turns out that the weight loss from exercise might be lower than you think. A review on metabolic adaptations to weight loss in athletes stated that low energy intake and minimal body fat are perceived as indicators of energy unavailability resulting in a homeostatic endocrine response aimed at conserving energy and promoting energy intake. So basically as you reduce your food intake and get leaner your body fights back and this is for a number of reasons like changes in energy expenditure, mitochondrial efficiency, and circulating hormone concentrations. All right Dylan but I don't need a study to tell me that weight loss is hard okay. All I gotta do is look in the dang mirror when I'm wearing a skin suit to tell me that something ain't working. Just get to what I need to know already. All right let's jump into it the strategy I'm talking about involves calorie density. Calorie density is just as the name implies. How many calories are packed into a certain proportion of food and calorie density varies wildly.  Wow such great insight Dylan potato chips have more calories than lettuce. The point I'm making is that the calories in the food we eat varies dramatically and if we focus our diet on low calorie density foods then we don't have to reduce our portion size in order to reduce the calories that we consume and we can say satisfied and satiated with the amount of food we eat and we don't have to starve ourselves. So what do low calorie density foods look like? As we can see from this calorie density chart the foods with the lowest calorie density are vegetables averaging just 0.2 calories per gram and then fruits averaging just 0.7 calories per gram. These are the two food groups you want to focus on not just because they're extremely healthy but also because they have the lowest calorie density meaning you can eat a lot of them without gaining weight. You want to lose weight then bump up that fruit and vegetable intake. Okay so this whole calorie density thing is great in theory but does it have any science to back it up? Has it been put to the test? A study on energy density of foods effects on energy intake took 18 subjects and provided them with meals for two days during three separate testing sessions. In these three sessions the subjects consumed either low, medium, or high energy density or calorie density meals. The results showed that significantly more calories were consumed for the higher energy density conditions even though the amount of food by weight was relatively similar. Calories consumed were around 1800 calories for the high energy density group and below 1400 for the low and that's the key here with energy density. You don't have to reduce your portion size or starve yourself to lose weight and 400 calories is the kind of deficit we're looking for to have consistent gradual weight loss. The study concluded that energy density influenced energy intake independent of macronutrient composition and that subjects reported no difference in feelings of hunger or fullness. Okay that's great but that was two days of eating. How does eating low calorie density stack up in the long term? A study on energy density's effects on weight change over six years took 186 subjects and measured the energy density of their diet. Six years later they found that the energy density of the diet had not changed for the individuals and that a higher energy density was associated with weight gain and a higher BMI. The study concluded that low energy density diets moderate weight gain and that lower energy density can be achieved by consuming more fruits and vegetables and these are just two studies out of many. A systematic review on dietary energy density and body weight looking at many studies concluded that their findings highlight the growing body of scientific evidence suggesting a relationship between energy density and body weight and that consuming diets low in energy density may be an effective strategy for managing body weight. Okay so lowering the energy density of your diet by increasing the amount of fruits vegetables and other whole foods that you eat will help with your weight but what does this actually look like in practice? A study done on salad and satiety took 42 subjects and had them eat a meal with a low, medium, or high calorie density salad before a main course of pasta. They manipulated the energy density of the salad by changing the amount of dressing and cheese they put on it. What they found was that a low energy density salad reduced the total caloric intake of the meal since subjects ate less pasta afterwards and what's crazy is that an even bigger salad reduced intake even further so more food less calories. The opposite was found with the high calorie density salad with dressing and cheese. Eating the salad increased the total number of calories consumed during the meal. The study concluded that eating a low energy dense first course enhances satiety and reduces meal energy intake and that consuming a large portion of a low energy dense food at the start of a meal may be an effective strategy for weight management. Okay Dylan all of this is well and good but I didn't see any of these studies use athletes. I'm a high performance thoroughbred race machine who won the town sprint at the Tuesday night group ride that one time when the fast guys took a recovery day not some quote normal study subject. Fair enough and guess what the inclusion of high amounts of fruits and vegetables is actually even more important for athletes not just because it'll lower the calorie density of their diet but because it'll improve the recovery after hard workouts because of the increased antioxidant intake. Let's jump into some science to see how various fruits and vegetables improve your recovery. A study looking at the effect of tomato juice on oxidative stress took 50 male track athletes and divided them into two groups a control group and an experimental group that drank tomato juice after their workouts. They tested both groups in a 12 minute time trial before and after and found that those drinking tomato juice ran significantly farther than they previously had while the control groups saw no improvement. The tomato juice drinkers also showed a reduction in markers of oxidative stress leading to the conclusion that the antioxidant lycopene in the tomato reduced oxidative stress and improved performance. These sorts of findings are all over the scientific literature. A study on lemon juice and exercise-induced oxidative stress found the same thing concluding that the lemon did not block the cellular adaptive response but also reduced cellular oxidative damage. Cherry juice has been shown to increase antioxidant capacity after a marathon leading to aided recovery and in another study reduced symptoms of exercise-induced muscle damage. That study showed that strength loss after eccentric exercise was 22% with a placebo but only 4% when subjects consume cherry juice. Blueberries are another rich source of antioxidants and have been shown to reduce inflammation after two and a half hours of running. Now notice how over the course of this video I haven't said anything about your macronutrient ratio meaning carbs protein and fat and that's because your macronutrient ratio is a whole lot less important to weight loss than you think it is. A study comparing weight loss diets with different components of fat protein and carbs took 811 overweight adults and assigned them to various diet groups with different ratios of carbs, fat, and protein which the subjects followed for two years. What they found was that there was no difference in weight loss between the different diet groups. They concluded that reduced calorie diets result in meaningful weight loss regardless of which macronutrient they emphasize. If you're thinking about your diet in terms of macronutrients then you're thinking about it in the wrong way. Carbohydrates could come from sweet potatoes or candy. Fat could come from walnuts or hydrogenated vegetable oil. What you need to do is focus your diet on whole foods not processed ones and in particular the low calorie density ones like fruits and vegetables. That's the science on how understanding and applying the concept of calorie density to your own diet can help you lose weight.  Not exactly groundbreaking stuff, it's pretty common advice that we've all heard before and that's because it works. It is in a fad diet. Now what are some practical tips that you can use to implement this in your own life? For starters stop thinking of vegetables as your side and start thinking of them as your main course. When you prepare a dish start by thinking about what vegetables you're going to use and then add in the other food groups and make sure vegetables take up the majority of your plate. Having a salad with every meal is another great way to lower your calorie density as we saw just so long as you don't use high calorie toppings. Use fruit as your snacks throughout the day and after rides to capitalize on those antioxidants for aided recovery. My go-to after a hard ride is a bowl of oatmeal with a high antioxidant fruit like berries and be sure you stick to whole foods. As we can see there is a huge difference in calorie density between processed and unprocessed carbohydrates for example. Finally you want to lose weight gradually instead of all at once because that'll help you keep it off and large calorie deficits will compromise your performance and recovery. Make sure you're losing weight before your competition season not during and make sure you're well fed for your high intensity workouts so you can get the most out of them. Thanks for watching I hope you guys found this information helpful if you like this video be sure to give it a like share it with a friend and subscribe for more training tips. If you want to see more coaching content be sure to follow me on instagram twitter and facebook and if you want to follow my training leading into this upcoming season be sure to check me out on Strava. Finally if you're looking for a coach shoot me an email at djohnsonattrainright.com


So a lot of you asked for this device, which I didn't think you guys would be interested in, but I'm talking about the Galaxy Tab S8 Ultra. We've done the unboxing on the channel, you can check it out, but this is all about gaming on this device. Now we know we have the S-Pen, and it works really well, it can do a lot of things with it, but we're not talking about that here. We know it has DEX, and if you wanna check that out, go check out TK's video, he's got some really good tips on this. But what about the gaming experience? Now, I could start lamenting, but let's jump into some gameplay.  Just let's jump in. And the flag is up, offside. Well, that's just a lack of awareness. He was clearly in an offside position. Here it is, Steve. Come on. You did it. Let's see what it dropped. That's the right way to use this tablet. It's a 14.4-inch tablet, it's massive, right? And those games looked absolutely gorgeous. FIFA looked great, it looked sublime, it looked really smooth. Forza Horizon was smooth as hell, as you would expect. The gaming experience here was really, really nice and absolutely enjoyed it.  That's what you're getting with this lovely display from Samsung, and also this great real estate that you have right here. Now, in terms of audio, this thing really takes the cake. Now, we listen to some, of course, copyright-free music, but also the gaming experience and hearing the sounds really clear. That's because of the cord speakers. They work really well, and you've got Dolby Atmos built into it. Now, some of you will ask, okay, we had that with the Tab S8 Ultra from last year. Why should I care this year? Well, I think it's the fact that when you pick up this tablet, first of all, it's so much lighter than what we got from last year, feels really comfortable to hold, and is just really nice. Very vivid, very sharp. You can also use your accessories from last year, like the Fullio keyboard case and stand. The one thing to notice, the camera hump on the back is actually larger than the case itself, so it doesn't fully fit, even though you're S-spin everything in its house, it may fall off while using it. Speaking of the cameras, how good is that front-facing camera? Yeah, this is what you get with the front-facing camera on the Galaxy Tab S9 Ultra. Max video resolution is 4K30, no 4K60, but to the end of the front camera, so you know what, there you have it. Okay, so Dundee, you've told us this is great for gaming, console games, remotely, Xbox Game Pass, that kind of stuff. What about my Android games? What's my experience here? Well, I'll put it this way. Android gaming on here is really good, but also cumbersome. For instance, Call of Duty Mobile supports controllers, plays excellently, you will dominate and terrorize people with it, as I did. It looks good, it plays well, absolutely gorgeous, and of course you're running, of course, eight and nine frames per second, no problem. PUBG Mobile doesn't support controllers, so using the touchscreen, it's more difficult to play there. Same thing with Genshin Impact. Now mind you, in terms of performance, you're gonna get great performance on this. It's got the Snapdragon 8 Gen 2 for Galaxy. Look, benchmark-wise, you know what's gonna get. On the tablet, it's much better because you've got improved vapor cooling. So you game for a while, and honestly, my temperatures did not go past 97 degrees. That's it, again, 31, 32, something like that. Degree Celsius, so performance-wise, you're going to get great performance from this device. Overall, I like the Tab S9 Ultra. I think this is something that allows me to do a lot of remote gaming at home. Quite effectively. In terms of a travel tablet, it's a little too large for me. I think it just feels like I'm carrying the screen of a laptop because it's 14x4 inches. It's rather big. But when it comes to home use and gaming at home, this is perfect, and I think this is the tablet that is for people who are looking for that. Or just want the best maximum Android gaming tablet. Now if you're looking to pick this up, and you're watching this before the 11th, our trading offer is up to $650 for this on Samsung's website. So use the links down below to pick them up. Otherwise, guys, don't forget to like, share, subscribe, and always enjoy your entertainment.


Summer is here, and we're in what the Navy calls the 101 critical days of summer. With more and more people out and about in the hot sun, you always want to make sure you stay safe. One of the things I cannot stress the most is that during the summer season, make sure you stay properly hydrated. Water is still the best thing out there, nothing beats water. The National Safety Council has designated June as National Safety Month in an effort to prevent the rise in mishaps that come with longer, warmer days and increased outdoor activity. Some good tips to help prevent heat-related injuries are to use sunblock, wear loose-fitting clothes and a hat. And if you find yourself getting fatigued from being in the sun too long, find some shade and be sure to drink plenty of water. Reporting from Yacos Gnaval Base, I'm Petty Officer Brian M. Brooks.


What was the key to turning things around in the second half and taking this into the next? I think our defense got a little bit better. And Kevin just took over, to be honest with you. I think it was like middle of that third quarter to the end of the game. He was exceptional. But I think the defensive end, loose balls. You know, Jean-Michael Green still got a lot of rebounds, but I think we cleaned it up. We played a little bit harder on that end. And it resulted in a win for us. What's the communication like with coaching staff teammates? Knowing everybody tells you how valuable you are to the team, but needed to come off the bench once again and provide that spark and ball handling for that second year? For me, I just want to win. Steve knows that. I think my teammates, they've known that. So for me, that's the main important thing for me. I don't really care about all that other stuff that doesn't really matter to me, but I just care about winning. Tonight, we got it done. And I came off the bench tonight, and it is what it is. Greg Logan with New State. Chairs, the first half defense sort of looked like the second defense the other night against the Thunder.  We didn't give up. I think our resilience was top of the line tonight. We could have easily said, this isn't our night again. They're shooting the lights out. But I think we came out in the second half. We were determined to play our best basketball. I think we got some favorable rebounds. The ball bounced our way a couple of times. We knocked down some shots, and we got back in the game. Christian Winfield with the New York Daily News.  And then can you see that have an impact on the team as a whole, watching a guy like Kevin just get hit his stride the way he did? Absolutely. It makes everything easier, you know, because when Kevin's going like that, they're going to start doubling and then everything else becomes wide open for us. I think he saw that in the second half. He did a great job of moving the ball too. I think he had like 13 assists. And that just came from making the right play. And it's going to be like that. Kevin's going to get hot a lot of times. They're going to send doubles at him.  Alex Schiffer with the athletic. Hey, Tess, what do you think of Bruce's game?  Bruce was great. Bruce was great tonight. He's another guy that, you know, just wants to win. He plays extremely hard, you know, on both ends of the floor.   But, you know, that's what we're going to need from him. And that's what he brings to the table every night. Dave Lichtenstein with WFAN? I can't, I was just popping up on Bruce. The challenge that he had with Jamal, what does he do well that made him successful, relatively successful, and it's such a great play? He plays hard. You know, he doesn't take any possessions off. I think, you know, as a league guard, somebody on another team, I think going against Bruce is tough because he brings the same intensity every single play. You know, he doesn't really get tired. He doesn't take plays off. And he's physical.    Back to Michael Grady with Yes Network. You scored, you know, over 20 points again for the fifth straight game. That's a new career-long streak for you.  Obviously, we've seen your growth. How are you seeing the game right now? I think it's slowing down for me. You know, I've been in the league now for just my fifth year. I'm getting old. But I think just, you know, experience watching film, playing with really good players.  But, I mean, just the development that I've had since I've got here for sure. And last question. We'll go back to Christian Winfield with the Daily News. Hey, Kara, Steve said, you know, that this was a great win because he felt like you guys needed this for your soul. Those are the things that we need this far. So do you kind of agree with that? And would you agree with the assertion that it's been a little bit heavy maybe in the organization just as a team over these last few games? Definitely. When you get a win like this, you know, we've been searching for a win like this. We've been in close games. We've been lost close games. You know, we were in Memphis. We came back and we lost that one at the end. So I think, you know, games like this are huge for just confidence and just for the fight that we had tonight just to know that we can win these type of games. And I think it's always good for your mindset.


Commander Fleet Activity Sasebo, Japan Self-Defense Force and Sasebo City hold annual emergency preparedness drills. Joining this year are two Tilt Rotor military aircraft. The MB-22 Ospreys are a capability that the United States Navy and Marine Corps has been using for a short time, but the Self-Defense Forces will also soon have the MB-22 at the capability. So it demonstrates to the citizens the benefit that the Ospreys bring. With the additional benefits of Ospreys, Sasebo City, JSDF and CFAS are better prepared to take action when a disaster happens. Petty Officer Christina Coffey, Fleet Activities, Sasebo, Japan.


The use of information and communication technology, ICT, has become increasingly common in home care for chronically ill individuals. This paper reviews existing literature on the topic, identifying 107 studies that have been published since 2000. These studies show that ICT can be beneficial in providing increased access to home care services, as well as improving patient outcomes. This article was authored by Birgitta Lindberg, Karina Nielsen, Daniel Zotterman, and others.


Hello, welcome to today's lecture. So we were discussing about structure determination of peptide by NMR spectroscopy. So now, so essentially what we want just to repeat whatever we did in last class, we have a primary sequence, amino acid sequence and then we want to translate that amino acid sequence how this polypeptide will fold into three dimensional structure and this for shorter peptide like 20 to 50 amino acid how we can get the three dimensional structure using NMR spectroscopy as a tool. So that is what we were discussing. So we discussed there are some primary steps in NMR structure determination. First is sample preparation. So for peptide we need to synthesize the peptide or bacterially express this peptide and purified. The pure peptide has to be dissolved in a solvent which we want to solve the structure and then 10 percent of D2O is or deuterated solvent is added to that for locking the spectrometer. So that is our sample preparation. It has to be transparent and there should not be any like aggregated particles. So that we have to make sure that sample is absolutely pure and sample looks transparent that says that it is pure. For purity we can use orthogonal techniques like mass spectroscopy Maldi or even SDS-Pasiglian electrophoresis to see if the band is single and in Maldi you are getting a single band and that tells about the purity of the sample. The next step that we have to record a series of NMR data that we discussed last class say like a Toxy or Total Correlation Spectroscopy and Nozzy Nuclear Overhouser Effect Spectroscopy. Then couple of like N15HSQC, Carbon 13HSQC or Double Quantum Filter CoG. So all this dataset we need to record it and that takes a day or maybe 2-3 days and after that we are discussing actually the assignments of peak, how we are going to interpret this data. So we ended in the previous class at the sign analysis of the data. So now we will discuss that and today we are going to discuss more about like how you use this data to generate the restraints and those will be used for calculating the three dimensional structure and once the structure is calculated then we need to validate that structure, does it satisfy the dimensional plot statistic and all those and then it has to be minimum energy structure. Once it is validated and energy is all the angular constraints and the distance constraints are satisfied then this structure is good for submitting into protein data banks. So that is what we will be discussing today. So we were discussing about let us say simple thing that I was showing you assignment of a few amino acids like we just showed it for alanine. So let us see this is our alanine in a polypeptide chain. So this is alanine and let us write it down for glycine that is what we had written here, glycine okay. So now we are looking how toxic pattern will come. So here is our say spectrum omega 1, omega 2. So say around 8.2 ppm our NH peak will come. So here is my NH peak then we have a peak coming from H alpha.  So if this pattern and this will be say around 1.9 this will be around 4.4 or something like that this will be 8.2. So if we get this kind of pattern in a toxic spectrum we now we understand that this is alanine. Similarly for say glycine we can have around 8.4 or so and then we have 2 closer peak around 3.9 and 4. So this is pattern for glycine. So these amino acids have a typical pattern in the toxic spectrum and that we can use to identify in the toxic spectrum and that will be our start point or something. Then we need to combine this with nogie spectrum to walk along the backbone. So here I am giving for analysis of data. So now we take a valine. So here for valine what we have NH. NH will be correlated with say H alpha. H alpha will be correlated with say H beta. H beta will be correlated with methyl. So if we in a spectrum in a toxic spectrum we get pattern for this suppose I write it down. So suppose here is our NH and we get a peak for H alpha which is 4.5 then H beta 1.8 and then H gamma 1 and H gamma 2 if very close. So if you get peak something like this, this we know that this is coming from valine. Similarly we can get a pattern say for any of the amino acid like proline here. So proline we do not have NH right. So here that is missing. So we have here 2 peaks and can be here and all those. So here also if we get a pattern like this then we know that this is coming from proline. Similarly we can take it from some of these here. So NH that will be correlated with H alpha, H beta and so on and so forth. So these will be coming for a simple spin system. Each of these spin system will give a typical pattern that is in toxic. Now we want to see which is near what. So then we need a like distance based spectroscopy and that is no G. So if we do no G we know that this CH2 can show a correlation peak with this NH. Now if this correlation in the no G spectrum from this NH to CH2 we will see then we know that this valine is near to this residue. Similarly if we see some H alpha showing correlation peak with the proline this protons then we know that this is coming these two are near in a space and that is how they have a correlation peak in the no G spectrum. So that is how we try to identify from the toxic and no G how which residue are like how they are connected. So here just for schematic we have shown two residue I and I plus 1. So this one residue is I up to here and this is up I plus 1. So in toxic you will get all the blue correlation which is like intra residue NH to CH alpha and then to methyl's right. And in no G you can get same like in the same amino acid as well as the neighboring amino acid. So intra residue as well as intra residue correlation we can get it. So here intra residue means this CH alpha will be correlated with NH of next residue. This NH can also be correlated with the NH of next amino acid and that helps us in identification of the proximity residue. So in toxic we know that this is alanine, this is serine, this is say arginine or so but if we combine no G with this we can know. So this alanine, serine and arginine are near in space and that is how we can identify. So I will work you through some of those assignment protocol. So let us see I am taking a sequence of 18 amino acid which is GGLRSLGRKILRAWKKYG. So this is single letter code of different amino acids and this is say one peptide which is 18 more. Now we have a beautiful toxic spectrum. So at the moment let us for simplicity I just concentrate on this region which is NH, like H-aliphatic. So these are all H alpha, H beta and here are H-methyls. So say let me write it down H alpha, here will be H beta and H gamma and delta right gamma delta whatever and here are H-methyls. So only I am focusing on this region. So let us take here are my point pattern. So for arginine here is H alpha H beta beta to gamma. Now for lysine we have one here and then series of here and then you see intense peak coming from glycine. Now here glycine both the HCH H this H2 glycines are showing peak at the same position because their chemical shift is not too different and that is how we have only one peak for that. Similarly we can find again for arginine here is for isolation H alpha H beta H gamma 1 gamma 2 and similar all the way of methyl. Now for say tryptophan here tryptophan we have H alpha H beta lysine you have all those peak H alpha H beta H gamma H gamma delta. So and again all those for this. So different amino acid type now looking at this toxy pattern zoom tap toxy pattern we can find it out which residue it is but like this 7 4 9 does not only come from toxy. For that I need to do something called NOGY spectrum we combine it and in next slide I am going to show. But other than that if you zoom this region this region is correlation of NH and NH like in each amino acid at least one NH is there and this population here shows that NH and NH are connected. This gives very important structural information. Now as we have studied NOGY gives you distance dependent correlation. So that means this NH-NH correlation if it is shown in NOGY spectrum this is toxy spectrum but I will come to NOGY spectrum it is shown in NOGY spectrum then that gives information about the distance. Here in the toxy spectrum if this we have a NH-NH correlation that clearly says that the amino acid which has NH as a backbone as well as side chain can also give you here. But this is also like if you look at 7.2 to 6.7 these are from aromatic amino acid. Now aromatic amino acids are very important. So if you look at here YW these are aromatic amino acid and then they can give correlation of the aromatic side chain correlation and that is shown here. So NH-NH correlation will be shown here in the NOGY spectrum that I will show you. This is aromatic protons correlation that comes around this and this is coming from tryptophan and tyrosine. So let us combine this toxy information with a NOGY spectrum. So here what I show for the same peptide 18 amino acid peptide toxy is shown in black and NOGY is shown in green. So if you see NOGY as we discussed it will give you intracellular correlation as well as intracellular correlation. Now intracellular will be same like toxy but intracellular will be different. So if you look at here many of the green peaks are also similar to the black peak. So this is coming from intracellular. However these all peaks are coming from intracellular correlation not intracellular. The green black overlap is intracellular. Green alone is intracellular correlation. So now this green if you identify this green because this is distance dependent or a dipolar coupling based correlation. So we know which residue is near to what and that is what helps us in identify. And again this is NHH alpha section. So if you zoom this section same thing we have here. So let us start from anywhere. Now say here let us start from glycine 18. So as we say glycine H alpha will be very intense and this coming at around 3.7 or 8 ppm. So we know that this is glycine. Now glycine H alpha is showing a correlation peak with another amino acid here. Now so you see this is H alpha. So H alpha H alpha correlation from glycine 18 to something which is nearby. Now what could be nearby? It can be either 17 or 19. Now if you take this peak and go in this line now here there is a peak and this peak has another correlation and that we have identified. So this is Y. So this has a correlation which can be traced back to the Y kind of residue that is tyrosine residue. So now we know that Y and G are near in space. So now we will go back in this sequence and then try to look at where are our Y and G. So here if you here Y and G are this. So now by using this we can identify this is 18 and Y is 17. Similarly let us go to some other here. Let us see here what we have is 15 K. At the moment I do not know whether this is 15 or not but I know that this is coming from a lysine residue. So lysine also shows a correlation peak at this position. Both of these are overlap. Now lysine will show a correlation. Now we go back here and we find that there is a residue which can be identified as a tryptophan. So now tryptophan and lysine seems to be together and then we can look at here. So tryptophan and lysine are together that means this is 15 lysine this is 14 tryptophan. Similarly we can do exercise for few of more amino acid like here if you look at glycine. So here we had a glycine, glycine H alpha showing the correlation peak of H alpha and 8 H showing the correlation peak 8 R is showing correlation peak which is this and this shows correlation peak with this glycine. So now we know that arginine is showing correlation peak with a glycine type and arginine and glycine are nearby here. So that is 8 and 7. So similarly we need to do exercise for days and we can walk across the backbone to identify each of these peaks using Toxy and Noji spectrum. This takes time as you see there are several overlaps there will be several ambiguity. So by doing like we can then make use of this region which is NHH delta region and by using whole spectrum we can identify the peak coming from the Toxy and Noji how they are correlated by bond and by space respectively and that is how we can identify the amino acid number like 7 G 8 R 13 A or so and so forth. So this is also like you can find it out and that helps us in building a model. So now I identify that if you look at the NHH alpha correlation is happening in backward right backward means 8 is showing correlation with 7 13 is showing correlation with 12 and 18 is showing correlation with 17 and that is how we can look at like these kind of correlation we are seeing here. So this helps us in building a model how the Noji peaks will be assigned. Similarly if we see a long range NOE constraints like for an example from here to here then we know that these two amino acids are closer in space and therefore by finding several kind of these correlation one can build a model for the polypeptide chain that my polypeptide chain will be folded in such fashions and that actually essentially can be identified using Toxy and Noji and this was very established technique like 30 years ago almost 30 years ago Professor Anil Kumar is a pioneer who has developed this Noji techniques and now is a core of the structural biology. So he showed actually for the first time how you can use Noji for doing the structure calculation for polypeptide chain. So now this building model helps us in identifying and fixing up the assignments great. So that is what we discussed the Toxy Total Correlation Spectroscopy which correlates which correlates by scalar coupling can be used for intracellular correlation and Noji nuclear overhauser effect spectroscopy can be used for like intracellular correlation. And then if we combine this something with double quantum filter Koji this gives you this three bond correlation J coupling J3 and if you remember we have like we told that this J3 is correlated with the phi torsion angle using carpolous equation. So just in a moment I will just give you some hints for that. So DQF Koji you can essentially measure coupling constant right by looking at the splitting pattern you can measure something called Hn, H alpha coupling constant. So if you remember where are the Hnh alpha in polypeptide chain this is essentially Hnh alpha and this gives you torsion angle this. So if you remember your Ramachandran plot phi and psi so this gives you phi value. So J3 is carpolous equation J3 Hnh alpha A cos square phi plus B cos phi plus C. So if you know J3 which comes from Hnh alpha experimentally determined Hnh alpha you can calculate the phi torsion angle. So here is the phi torsion angle this is psi. So you know this is beta strain region and this is alpha helix region and this is L type of three prominent region. So if so just by knowing this J value one can know where my polypeptide what kind of conformation my polypeptide chain has and say for a random coil say typically it is a 6 hertz or so. So 6 hertz is for random coil chemical shift which does not have any persistent structure. If this J3 moves towards 11, 10 to 11 hertz then we are in beta strain region and if it is goes like 3 to 4 hertz then we are in alpha helical region. So just by looking at the J calculated for this double quantum filter cosy we know where my polypeptide chain is. So suppose we are getting this J3 around say 9 to 12 hertz then my polypeptide chain is in this region. That means if you remember the value here is 0 this is 180 minus 180 plus 180. So one can calculate what is the phi and psi just by looking at what is essentially phi just at looking at the value J. So experimentally determined from DQF posing double quantum filter cosy you can experimentally determine and you can guess the conformation of polypeptide chain already. Now so now we have got the assignment of peaks we have got through a space correlation we have got some idea about di head draw the strains coming from the DQF cosy. So that all will be used in generating the these strains. So these strains now can be further used for calculating the structure. So two things are important one is torsion angle how are polypeptide chain what kind of conformation I have and then another is distance resistance how these protons in space are correlated if you go back here the correlation that we have shown how these will be correlated what will be the distance from here to here here to here here to here here to here. So if you go to get this distance and we get the torsion angle is phi and psi phi and psi then one can find it out the conformation of a polypeptide chain. So these two things we need. Now over the years people have developed algorithm which is called Telos torsion angle likelihood obtained from the shift chemical shift and that that can be used for calculating the phi side dihedral angle. So this was developed by a professor from like his group in NIH at BEX there his group developed this algorithm called Telos or Telos plus which uses chemical shift that you have assigned from Toxy and Nozy to predict the phi and psi torsion angle for each of the amino acid. So what it does basically Telos actually Telos takes the chemical shift for 3 amino acid in a polypeptide chain. Suppose we have a ARS W Y G L something like that. So suppose I want to calculate the phi side torsion angle of this alanine then Telos need 15 chemical shift as an input phi from here, phi from here, phi from here. What all chemical shift it needs? So it needs H alpha, C alpha, C beta, C O and H N. So these are five chemical shift it requires and then what it does it matches with a database like already so many protein structure has been determined. So now so it matches this chemical shift in the database base and also this stripe peptide sequence in the database and then from the search it gives you information of these phi psi that were there for all the structure determined. And then it predicts approximately with a range say 120 plus minus 10 this should be the say for psi this is 120 and for phi say minus 120 plus minus 10 some something like this it predicts and gives you value. So that you can use for a calculation of the torsion angle. Now as we have seen that NOE intensity from each of the peak that has appeared depends upon the distance R. So if we determine the intensity of these peaks that we had earlier here like now we know that these peaks come from the correlation of which two protons from two different amino acids we can find it out the distance of those amino acids. So intensity of NOE between i and jth peak determine depends upon 1 by R to the power 6 and that gives you actually distance correlation. So you can now use this dihedral constraints obtained from either DQF cozy or obtained from Telos. Then you can use this distance restraints coming from the NOG peak intensity and then one can generate a template. Template is essentially a random coil chemical shift. So residue name the atom number then list of bound length and bound angle and then one can generate a random coil polypeptide change this does not require anything and then one can fold through molecular dynamics and then what we are doing. So we generated a polypeptide sequence like this. So right using the chemical shift and all those now we have a angular constant. What should be angle here all these angles and we have a distance from here to here, here to here, here to here all these distances we have. So that is what you need. You need a template, you need an angle that will be required for folding and you need a distance. If you use this experimental constraints coming from the torsion angle distance coming from chemical shift data obtained by Telos or DQF cozy and distance restraints coming from NOG data then we are all set for structure calculation. So what we do something called simulated annealing. So we start structure and then this structure is heated to high temperature in a simulation and then atom of a starting structure can get a high thermal mobility. So everything at high thermal mobility is quite flexible and then you do you cool it in a step wise and then these constraints will be restraints will be used given by experimental constraints. So first you heat it then in an unbiased way slowly you cool it. Now structure calculation algorithm will use these restraints and then they will minimize the energy and then energetically favorable final structure will appear and that used by this whatever force field we are using. So energy minimized structure will come by this simulated annealing process. So here one can see. So you do restricted molecular dynamic simulation you get a template and then as you start so like distance geometry start from a extended polypeptide chain heat it at top and then slowly let it cool down. So structure will be revolving in a step here you can see at the end finally we obtained a folded structure. So similar thing is shown here starts from many conformation at high temperature slowly by using these restraints obtained from experiments and then making correct kind of context here the tertiary and secondary constraints finally a structure evolves and gives us a folded structure. So that is how you determine the structure of a polypeptide chain. So essentially it solves some of those equations. So it minimizes the distances and it minimizes the energy. So by using this ENOE energy finally by minimizing the energy it does the it gets the minimum energy structure. So one thing to notice here NMR never gives you unique structure it gives you ensemble structure those which has minimum energy. So the if you look any of the protocol that is used either Sienna or Explorer in NIH that gives you either 10 ensemble structure or 20 ensemble structure these are minimum energy structure obtained by this distance geometry simulated annealing protocol. So nutshell what we have done now starting from the polypeptide chain using we did MD simulation to calculate it use the restraints for force it to correct like context then we get a 20 manner and minimum energy structure and from there you can take one of the representative structure that will be a structure of your peptide. So if you look at here starting of the same 18 amino acid that we have started there are some amino acids which shows a helical and some appears as a tail. So that is what the energy structure determination protocol based on the minimum energy is used for getting these kind of a structure. Now the next step is to validate this structure. So for assessment of the quality of a structure one has to use this protein structure validation suite. So essentially what you need to do so provide the standard constraints analysis and a statistic of PDV validation protocol and then these structures will be tested against already solved structure using these protocols and then it will quality score will be given for your structure and that will be suitable integrated with the database. So what we are doing just for our obtained structure we are checking against the already solved structure from the PDV that does it fall. And finally you need to check the stereochemical quality of your protein structure that means you have to do the acid test for your solved structure by putting it in the rama chandran plot map whether all amino acids are falling in the rama chandran plot map or not. So if you do that so you can find it out like your this some of these protocols will find it out how many residue or what is the reason in the favorable reason of the rama chandran plot what is in additionally allowed reason. So if you look at the previously determined structure most of them are either in allowed reason or additionally allowed reason none of them are essentially in disallowed reason that means this structure is very good so quality of this is very good. And rmsd is root mean square deviation so deviation of C alpha atoms of those 20 amino acids is very minimal that means structure that has been solved is very very coherent and you can see it from here all these structures seems to be overlapping on top of each other these are 10 minimum energy structure ok. So if you do that we have final structure determined. So to sum up what we did here we started with a sample dissolve that in a proper liquid at 10 percent of dto then we take it to the high field magnet or whatever magnet that you have a core data for days then we sit together and do the data analysis for weeks and then we calculate the structure and that will be final representative structure of a peptide and that is what and then we need to validate it do the pro check analysis to see the structure is of high quality if we obtain this we are done we can submit in PDB and that will be our final structure determined from NMR spectroscopy using solution state protocol ok. So I hope I give you glimpse of how you determine the peptide structure using NMR tools. I hope this will be useful for you and if you have any doubt please do write to us ask us we will try to resolve thank you very much.


Hello and welcome back to the craft box. My name is Brenton today, we are over in Turian Gorge doing things and stuff I'm just gonna talk to Captain Kroger all below and I'll be back in a sec. Just let's talk You stink like an ancient moth's ear. Don't you ever bathe? Why bother me? We're recovered from several dastardly burrow attacks. Probably the work of this Zaltana said I was to help you. Help you do what? Find the Moonstrown in the Winter Halo Cavern? Huh, a fool's tale. But search if you want. The caverns lying near the milkweb gate. They're infested though with winter scorpions Okay, so Let's hit on in here Um, I don't know what's in here, but we will find out in a bit Um, so basically in between not in between episodes last episode I um Um, last episode, uh, we defeated The brood mother and a thing else. He was like spooky. I don't really know. Um, oh gosh. Yes, you're about to go in Yes, okay. Cool. Whoo Um, so yeah, let us go real quick on this We're gonna go pretty quick for this Um Okay, he gotta go soon. That's cool. Um, oh my gosh What is these defeat winter scorpion?  Um, what we did last time was we defeated moth. It was a long episode. Go check that out It was great. Um, if I say so myself, which I do um So yeah, go check that out and today we are basically entering gorge again again I know we've been here so many times. We're gonna go back again Um, and we're doing we're basically finding the moon crown is essentially what we're doing. We've still got the help of A kyle again. I've literally started recording after the last one because I have to record like a billion videos today So, yeah, fun. Um, so if you haven't checked out the last couple videos, go do that. It was great. Um, happy happy Happy christmas eve. Yep, that's all along the 24th apparently. Yep, that's good Um, so happy christmas eve of that time and or if it's the 23rd for you in america Where's it probably would be because it goes up in my time, um, which is earlier. Um, happy happy Christmas eve eve Yeah, so yeah, that's fun with that one. Um, but yeah, basically Lots of things happen and lots of things will happen today. Probably not as long of an episode, but we will see Never know what happens. Um, kyle has to go soon. So we'll probably return to soloing later. Um Yes, uh, we're gonna hit with tenite. Yes, boy Yeah Okay, this should do a decent amount of damage. Hopefully, hopefully from this crust um, we boost on the Balance and we don't boost on the ice, but that's fine. We will still do a decent amount of damage if we correct No, okay, that would do a lot Yeah, 2000 is still good. Like that's great. I'm impressed. Um, yes So that's fun. We are doing a whole lot better. They can know what we're doing Boss sort of situation, which I think it is from reading things. Who knows probably wouldn't be surprised. Um I mean, that's kind of how chrysalis has kind of been going so far. So Um, we're also gonna be getting shadow magic sometime soon, which is fantastic Um, and that will I think maybe for the end of the year Um, so in the next couple videos I have to record. Oh scorpion squish a hello. That's an interesting question Oh, shit didn't realize how we did didn't put prisms Oh no prisms Yikes Yikes, um Got pulled didn't put prisms in Okay, I hear like when Because if you don't know it's actually summer and um In new zealom, um, because we're in the southern hemisphere, of course Like winter has like all the snow and fun with it and we just have pot boiling with it. So, you know Um, but yeah, it's like boiling today and I like hear random noises like Window it's kind of weird. I don't know why it's kind of spooky. Um, but you know A bit of happens will happen um So, yeah, we will probably just blade and hit in a minute. What just to get extra damage, but we won't do much at all Um, we'll probably we'll definitely just let him hurt. Um, because we can I'm still bleeding. You know, I just pass. That's just not cool in these days, you know Can't be one of those. Oh, I'm just gonna pass Okay, lots of blades and lots of fortify Um Sure, why not just for lots. They don't actually have much health like that's a surprise Why not it's cool Hahaha You know, it's fine. It's look at that. That was bad. I mean, it's actually like pretty good considering the other one did like Nearly the same. So, I mean Just for fun because why not Burn them, burn them good. I like how I did the one that doesn't have the fortify I don't even know why I did that, but you know, that's cool Um, here we go. Frost Ryan, so that should be the end of that battle. We also have a boss I will need to remember to switch my deck Probably a great idea Um, hope that I can have time to I don't know who this boss is. Um, who knows I see now you are an agent of light an arrow aimed at the heart of shadow You seek the moon's crown. It is still here The moon I stopped the moon's crown is a prize of the baleful empress if you overcome her My spirit will fly free on the winds of change. Okay. Sorry I skipped all that. I know But warm blood enters my home. I had a great feast for my children I really love this layout. It's like crystal caves and Avalonics set like on steroids. Um, okay Let me just put in a couple of fakes What's this? Oh, hello treasure card trade Crip corn strip. Oh, hello Okay Summon it round one Okay, we will do that then It's like monstrology yet as we will discard these I'll put a faint in a thing and Okay Good job Brent. How many pips is it? Is it one? I don't know. I didn't even see Two pips. Okay. That's cool. I can do that That's cool. Um, so yeah, we'll do this boss. Um, should be good Not too bad baleful empress Storm, um, scorpion. What is the badge for scorpion? Like where was that? Was it a large badge? Maybe imperial color Maybe I don't know Anyone Sure I hate sirens And draw please Of course we got this thing So this is monstrology for you. It's from Raj Yeah, um Yeah, the boy is fantastic. Look at that amazing is nice It's a buzzer.  Oh Oh Um, yeah, so these are from Raj. They're like really cool. I love the model. They look fantastic Amazing Like choking Don't make weird sound Brent. You choke. Um So, yeah, these will hopefully speed this long. Um a lot monstrology is great for this sort of thing and People bash it but like work sometimes like this. It's pretty cool. Uh, blade and humongous frog. Okay If they like bladed and humongous frog that would have been cool Should I like blade him? I'll blade him. Why not? I might as well see what they do. It's enchanted as well. Maybe I should have done my enchanted one. Oh, well, it's fine We'll see. It's fine. He'll do a lot of damage We're not at all We'll see how much this does So that's always a good start plus a blade like whoo. Hello, and it boosts as well. So he's probably dead, right? I mean, I could have waited and like That was just a one-time thing obviously Uh, I'm gonna say we're not gonna blade him. I just mean to do a lot of damage. They sure do I'm not I'm not blading him I'm blading myself. Well, let's see how much this does Oh So, yeah, this is Just using mirage minions to our thing gotta love it gotta love it gotta love it Well, we're gonna blade again, can we? 45 year we can cool um Fantastic Nice, we faint there. I can do another one as well. I'll probably do a potent next Just for lolz Actually, he's probably gonna hurt, which would be good Actually, no, he's got a lot of health. So maybe not. Oh fizzle unlucky Oh unlucky Temp gotta love temp temp temp. It's quick. So I like it. You see Okay Fantastic and what are we gonna do now? I can probably faint Confane to start my linemence for one round Oh dear, no, just because I can You know just cause I've got two blades three blades. It should be fine We'll do a decent amount of damage. There's also humongous frog coming as well. So we should be fine Hello, this will do a lot quite a lot probably 50 45 70 Kaboom Um, okay one two three blades carb I'm surprised. I'm surprised. I'm gonna give you that. I'm a bit surprised that didn't That's not sure. Whoa, he can be stunned. I some bosses just can't be stunned. That was really rude, but like Whoa, it can be stunned. Oh, it's pretty good. Um, okay humongous frog Okay Uh pass fantastic. I mean she has no pips. So she's also stunned Um I'm just gonna pass. That's good They're good. They're good Wrong there, but I don't care It's just a majestic sound like whoa They're like trying to change stun. That's pretty pretty loot pretty pretty good, you know I think they have 70 Wow, that's a lot That is Impressive, I don't care how to spell. Who cares about spelling Wow, they're pretty good They're good. They're good ones. They're good Okay And a frog hello frog hello Frog is great The only spell that myth has that is good at alie until, um, dogma So true Hush, but true Crut hello A lot still for like yeah They're good monstrology good. No, not really. Um Okay, we're just gonna I'm getting all like faints I have his faints. Uh Yeah That's gonna be fun, um Okay Let's see. We have that finally, which is nice. We don't need you Oh king art. Hello. That's cool. No enchant. Unfortunately. We are going now that we're gonna faint just in case You'll probably just in case who knows who knows what will happen I don't that's for sure Um, I mean I could have saved pips for dr. Von, but it doesn't Uh Hopefully Medusa that would be nice. Oh, of course Of course Well, if he's not If he's bad if he's going to be annoying then I'm going to do day and night Fantastic Chip in the crown a precious stone is missing Still with this in hand you are well on your way to driving back the shadow Thank you spellbinder for freeing me Okay, fantastic. Um, let us hit on back Um through the portal Fantastic And then where are we going over here apparently oh gosh those things don't want to remember those Whoo, they were annoying. Um, the death as well. So it's even worse don't like them. I think he's over the this side Yes, possibly Uh, yeah, yes, it is. Okay, cool Um, hello Hello Stick of scorpion venom Oh, you found the moon's crown, huh part is chipped. Oh, I wouldn't worry You can barely notice that I'm sure we'll do just fine Take it back to salt on and bastion and you tell her to find out who this Cornelius is these spies cannot be tolerated Okay. Oh, we get a scorpion pit that's interesting. I didn't read that. Whoops imported before I get read I think I can do a few more quests Okay, cool Spellbinder you have recovered the moon's crown Your deeds are quite I'm sure the shadow queen will take particular notice of you soon If that is your wish Cator told you to investigate the spy Cornelius. I would not worry about that I do not think such a person exists There has long been a rumor of a fifth column within the umbra legion This treasonous group supposedly believes in a different reading of the shadow queen's grand prophecy But this is harris any who claims such a thing would face unimaginable punishment if they were to be caught Who would dare but to matters at hand who sent you after the moon's crown The brood mother how curious take it back to her at once Okay, I'm hitting back to the brood mother And then I think we're gonna end off the episode there um Just because it's getting kind of long spellbinder I struggle to keep my thoughts my own Do you have the moon's crown? I have been a buzz since you left barely able to contain my emotions I fear the shadow queen's wrath, but I cannot live like this. Do you have the moon's crown? I see it as broken and missing a gem But it still has much magic within it Bring the moon's crown into the royal queen's chamber and let us see if we can master its use Okay, um I'll be back next time and we will continue on so thank you for watching And to leave a like and subscribe and if you're very subscribed press the little bell So you know exactly when I post another video and remember craft outside the box


My name's Jay Nichols. I'm a marine biologist from Davenport, California. My talk is about BlueMind, which is the title of a book I wrote by the same name, but also it refers to the way we feel when we're near and on or underwater. I call that BlueMind, and it's a different mental state. It's a different emotional state that the water puts us into. And I refer to water in general, not just water in float tanks, but lakes, rivers, creeks, pools, tubs, the ocean, and all that water does that to us. And so I like to explore and go a bit deeper into the science behind what happens from the moment we touch the water to while we're floating in it to maybe swimming or going under water, or perhaps even just looking at a photograph of water can take us to that BlueMind space.