for you. So our next speaker is going to be Dr. Wolfgang Baer and he is our, he's a professor here, but he's also director of research here at Moran Eye Center. And so it's a pleasure to have you talk and he's going to update us on some of the important research that he's doing. So we change topics quite a bit. I'm a biochemist, molecular biologist, and I think I was invited to present here because I do something translational. My topic is stereopathies and I, my protein or my gene is called IQ CD1 or MPHP5. Sounds very complicated. It stands for IQ domain containing protein B1 or nephrocystin 5. This protein, when mutated, causes senior local syndrome in patients, in human patients, and we are producing mouse models to replicate or see differences in mouse, in mutant mice with mutations in the same gene. So senior local syndrome is an autosomal recessive disorder characterized by nephron of thesis and progressive retinote generation. The worldwide prevalence is estimated to be one in a million, so it's relatively rare. The disease was discovered, like more than 50 years ago, in papers published in 1961 by Dr. Seymour and Dr. Logan, who gave them the name of the senior local syndrome. There are only six genes involved in senior local syndrome. They're called MPHP1, 4, 5, 6, and 10. One is still unknown and I have two students working on those genes. One is Christine Hunker Gokokia, a graduate student from Germany and Michelle Reed, a graduate student of the neuroscience program at the University of Utah. Nico was an empty PhD student in my lab starting the project several years ago. So what is senior local syndrome? In human patients you see diffuse interstitial cell infiltration, dysphiprosis, tubular epiphy, cyst formation, and end stage kidney disease is around 13 years of age. In mice you see mutant kidneys become smaller, shown here. The middle is reduced and cyst formation can also be seen. So it's a mouse, nephronophtesis, not generated by one of my favorite genes. It's another gene which does not cause retina degeneration, just nephronophtesis. So the high disease is retinitis pigmentosa, or LCA, labor congenital amoresis, and it's pretty well known to most of you. I'll compromise peripheral vision, tunnel vision, abnormalities in RPE and cones, and eventual lead in life blindness. So I just made this picture to show you the complexity of the nephrocystine genes going from 1 to 19. In all those different colors are different domains. In proteins you see there's virtually no relationship among all of those proteins. There's no sequence similarity and the function of all of those proteins is unknown. The proteins causing nephronophtesis, causing senorocrancy storms in yellow, and the other proteins, the other mutations in those genes, cause mechal syndrome, butaspeedle syndrome, and churir syndrome, which are synoptesies, syndromic synoptesies. So I'll just give you a very short introduction to the photoreceptor nomenclatures, so they are all on the same page in the rest of my presentation. Our photoreceptors are having outer segments and inner segments. They are connected by what was previously called a connecting selium, and it's now named the transition zone. The transition zone between inner and outer segments. You see there are lots of disks containing the photoreceptor cascade. There's an axon name, which is basically the backbone of the outer segment. I made an enlargement of the axon name to give you all the nomenclature we use in the next few slides. The axon name is a singlet microtubule arrangement. The transition zone is a tuplet microtubule, and there's a basal baldy, which is one of two centrals in the cell. The basal baldy, which is important for generating transitions on an axon name, and then there's a daughter centriole. So also important is photoreceptor outer segment development after birth from about three weeks of age. You see that at the neonatal baldy, the basal baldy comes to the cortex of the cell, and at P3, it pushes a little bit of a membranous bubble here, which will be the transition zone shown here, and eventually you form an axon name shown in blue. So this takes about three weeks from birth in mouse, and we are mostly interested in mutations that change this arrangement or prevent outer segment formation. So the basal baldy, as I said, is very important, basal baldy and axon name are very important for the outer segments' cavity. Without them, the outer segment would be very instinctive. So this shows you, it's taken from an independent paper, shows you that all those nephrocystins are closely connected, either directly or indirectly, forming an elaborate dethrone in the proximal cell here. This would be the basal baldy transition zone in bursing compartment and the axon name. So now we get to my protean, and you see it has several domains. One is called an IQative, which is a thermo-related binding site. SCC is a colloquial region for protein interaction, and ACCP is a site for interaction with another nephrocystin. So multiple non-mutations are associated with senior local syndrome as shown here, throughout the molecule. So what, since the disease is recessive, we were planning to make a knockout mouse, and for a knockout mouse, we would insert a gene type in one of the introns. The gene type basically prevents translation of the molecule shown here. So it stops like this. A gene type is a functional protein cannot be made, and evidence that we have knocked out, the gene is shown here in the control. You see the nephrocystin-5 accumulating at the transition zone, and in the knockout mouse, the protein is absent. So look to electrometanography at P14, when mouse eyes are opening, you see that the mouse is blind, has no response, same as P18. This is a scotopic for rats, and photopic for combs. Again, combs are not active, and the mouse basically is blind at birth. OCT can be used to look directly at retina, and you see that in the knockout mouse at P28, one mouse of age, we see a LCA type, or retinine-specific dosage type, phenotype, vestige generation of rats. Now this gives you an idea how odopsin is developed, and how other segments are developing with odopsin as a marker. Odopsin is the visual pigment in the photovisible odor segments. At P6 and P10, which is before eye-opening, you see slowly developing odor segments, and after eye-opening at P15, odopsin and odor segments are forming very strong. In the knockout, in contrast, you see no odor segments developing, odopsin mis-lokalizing in the inner segments, and eventually a rapid degeneration, and you see essentially one wall of nuclei still present at P30, which most likely is combs only. So degeneration can be assessed by looking at the thickness of an outer nuclear layer, which is only for the receptors, and you see there is a relatively little degeneration in the first two weeks of age, but after eye-opening at P14, there's a rapid degeneration at one month. Degeneration is mostly complete. So to our surprise, kidneys are completely normal. This is a kidney section at one year of age, and control and the knockout, you see there is no difference. So uterine kidneys are completely normal. This was assessed by Dr. Patricia Avello at the ARUP here at the University of Utah. And so there's no default of thesis in our mouse model, so it is not a is not a syndromic synopsis.  And kidney cilia, as you see here, are completely normal in the mouse. So cilia and kidneys are not affected, explaining why we don't have any form of thesis. And this is a picture of mouse and fibroblasts, and these are very important for development during embryonic development, and you see they have cilia in the controls, and they have cilia in the knockout, basically unchanged, consisting of patient body, daughter, son, and cilia. If this cilia would not be formed in the control, the mouse probably would not survive and would be happy on the glissom. So this is a reason why we have life mice. Now, looking at mechanisms, why do we have degeneration? We decided to force in sections and assay the retina. And you see a force in section here, O and L in a segment, the transition zone, and the outer segment, the base of the proximal part of the outer segment. And you see something green in here. This is a marker, each EFP-centered tool, calcium binding proteins, interacting very strongly with the transition zone and centrioles. So to close it up, we made an enlargement, and you see a transition zone is formed, here's the basal body and the daughter centriole, and these are the cells. In the knockout, we don't see, we see the basal body and the daughter centriole, but we don't see a transition zone explaining why we don't see an outer segment. And this is a second experiment with a protein called Parminium-1. Parminium-1 is located at the base of an outer segment, and it's responsible for formation of discs. And you see in the knockout that this protein mislocalizes, it doesn't form the disc, the proximal disc, and again consists of this lack of outer segment formation. On that ultra-structural level, we confirm that the control of retinal forms, here's the basal body, forms a very nice transition zone and a little bag of membranes, which eventually develops into another segment. And in the control, in the knockout, we see a basal body and a little bit of an extension which resembles a slanted transition zone that is not a normal transition zone. And at a later age, at p10, we see outer segment formation in the control basal body transition zone, axonene outer segment, and there's an outer segment in the NPHP5 knockout mobs. So what we have learned is that the NPHP5 knockout was made, the granular phenotype is LCA, a non-syntromic pseudopathy, degeneration of mutants, what photoreceptors is complete at one month. Mutant kidneys do not develop nephronophysis, nodal selia of NPHP5, photoreceptor transition zones, axonemes, and outer segments are absent. So we would like to know more details about cones. Cones are important for daylight vision. If we can figure out a way to rescue cones in those mice, maybe vision can be restored. So the way to go for cones is to make a double knockout mouse with an NML transcription factor. NML is neural retinal lucency transcription factor. It is required for what development. If we get rid of this gene by a knockout, we would make a mouse that develops only cones. So that's of course a very nice experiment because in the wild type mouse, we have only 3% cones and 97% rods. So we can now with this mouse focus on cones. And you see here a development of cones from P15 to 3 months into controlled mouse. And you see again our centrioles and transition zone and the outer segment, the cone outer segment forming. And in the on the NRL level and the ONL level, you see a beautiful stick ONL. This is only cones not rods are eliminated. And in the control, you see our two centrioles, no transition zone. And surprisingly at three months, the essentially low teach generation of the cones. So we have a stable situation of at least three months where the cone teach generation is basically very, very slow. And we have a window of three months or longer for gene therapy, for gene replacement therapy and rescue cones. So this is shown here again. Looking at ONL sickness, it doesn't change for a period of at least three months. Not significantly at least. So in these are the experiments to come up with a virus for gene replacement therapy. On the left is a vector for transfection of tissue cultural experiments. And this would be a shuttle vector for producing a virus that can be injected into the sub wetland space, expressing nephrocystin 5 and hopefully initiating cellulogenesis and auto second formation. And we have a window of six months to do this. So I mentioned at the beginning, we are trying to do a similar set of experiments with MPHB10. And MPHB10 is known to cause senior open syndrome in human patients. And so far we have produced a knockout. And you see the knockout produces a body beetle syndrome like phenotype. It has six digits and unfortunately the mouse does not survive. It's a neonatal mouse just born but not alive. So the future of those kind of experiments is to make a tissue wetland specific knockout and maybe design a second set of replacement therapies with MPHB10 mice. So this is the summary slide. MPHB5 nullally ill senior open cause senior open syndrome. Nullally ill same mouse effect only the retina. Kidneys are normal in the knockout and little rats degenerate within one month but cones are very stable and survive up to three months or maybe possibly even six months allowing for chain therapy. Here's my thank you slide. Grants from, I have two or one grants from the National Institute. Right now the research department Linus has an unrestricted grant for the department foundation finding Linus and that research foundation gave me small grants to support our grant students. Thank you. So Wolfgang obviously fascinating and indeed if we could figure out how to decouple rod degeneration from cone if we make that disease. I think the changes are very good. The chance are very good in mouse but I can't guarantee it will work in humans the same way because for mouse to humans a large step we would include a large animal model in between maybe a dog model. There is a dog model for senior open syndrome with MPHB5. So if we can do it in a dog model and we have mismodifications of the shuttle vector and the virus we may be able to get them. So I don't know if you mentioned but everyone, Wolfgang is our director of research as well as all of the other work that he's doing and we're just very proud of the outstanding work he has and Nico who he's talking about is one of our residents. Where are you Nico? Raise your hand. Nico here. I think he's on a call. He's not on a call. He said he's on a call. It shows you have this interesting combination of the people working together do interesting things. I actually wanted Nico to present his but he said he's on a call. We do that tomorrow. It's very exciting and you know it's how you've gone through this and the potential to change the cone side with in retrospect it's a huge blessing. So your mouse model of MPH5 is mostly LCA type but at least in humans it's not quite that bad. How do you kind of reconcile that? You know differences from human to mouse are very common. I have many genes which cause LCA in human and when knocked out there is no phenotype in mouse. So and there's a dog model with the same mutation. It's a frame shift mutation in MPH5 and this dog has a lid on it. So there is an axon in form. There's a transition zone and all this and another segment but then in each animal. So what came with the MPH5 mouse knock out why do you think that that gene is not required for kidney development or do you think that there's another gene that rescues the phenotype? The short answer is redundancy of nephrosistins. We've done this in so many nephrosistins and some of them can replace the function or substitute for the function that has been lost. So it's really depending on the tissue and on the cilia in brain, kidney, liver, but whatever. So it's a different situation. So another possibility in human therapy would be to stimulate another one of those proteins that kind of... Possibly if that would be a fantastic idea if that can be done, yes. Well thank you Wolfgang and thank you to all of our morning speakers. We'll break for lunch now.


This is stock number 11335. We are here at Summit Automotive in Fond du Lac, Wisconsin. You're new and used heavy-duty truck and RAM headquarters. Today we are checking out this super clean Lake New 2020 RAM 2500 Proo Cab Short Box. This truck has the 6.7 liter Cummins diesel. It has been fully safety and inspected by our service shop as a fresh oil and filter change. All the fluids have been checked and topped off, and this truck is 100% ready to go. Delmonico Red is the color we shoot all of our videos in 1080p, 60 frames per second, so if you have HD capabilities on your computer, tablet, or smartphone device, turn them on right now because it is definitely your best way to check out the quality and condition of the vehicle before seeing it in person. And if you'd like to check out all the photos on this truck in the upper right-hand part of your screen as a link to our website, click that and check us out there. The night edition gives you the 20-inch black painted alloy rims, no scuffs or scrapes on them, and it has Lake New Firestone Transforce LT28560R20 tires with just about all the tread left. Frame and underbody is an exceptionally nice condition. You get the blacked-out RAM logo, Cummins logo, and 2500 badge on the hood there. No denser dings on that front fender. You also get the LED lighting group, and LED fog lamps, head lamps, and running lamps. Front bumper parking sensors, no major denser dings on the front bumper. Looks really good. You get the gloss black grille with the RAM stamp logo, and the hood is in really nice shape as well. Didn't see any dents or dings on there. Passenger side front fender, no scuffs or scrapes, or dents, and the passenger side rim. Same thing, no scuffs or scrapes. As you go down this side of the truck, take note of how clean the body is, how reflective, and mirror-like that paint is. We take these HD videos, so if you are far away, or even if you're close by and you just cannot make the trip down, but you're still interested in purchasing the vehicle, you can see the truck, hear the truck, and have confidence in the vehicle that you're looking at before you even get here. So that when you do get here, there's absolutely no surprises. And that's why we offer the Summit Auto FastPass option in the upper right hand part of your screen. A link right to our website where you can apply for financing, get pre-approved, see a payment quote, even appraise a trade, all from the comfort of your own home. So when you get here, all you gotta do is drive the truck, love it, and you'll be all set to go. Frame and underbody is exceptionally clean, and it's like that all the way underneath. It hasn't been altered in any way, still has all the factory exhaust, and the lower rockers all look really good. This is a one owner, clean title history out of Iowa. I don't know if I said that yet, but it is. No accidents on the auto check. Rear bumper is absolutely perfect. Parking sensors, full towing package, which includes a receiver hitch, four pin and seven pin wiring. The tailgate is very nice as well. You get the LED tail lamps back here, the blacked out RAM 4x4 and Laramie logo. This one has a shock down assist on the tailgate, so it's never gonna slam on you. The bed's in really nice shape. Comes with the fifth wheel and gooseneck prep package. You get seven pin and four pin wiring back here as well.  Also side box tie downs. As you go down the driver side, just as clean as that passenger side, no dents or dings on the box. Notice you do get the fender flares with the Laramie package. And for full disclosure, this back rim is in excellent shape as well. Down the rest of the side looks really good. Get the enter and go system. This one has the heated folding, fold out tow mirrors. They have blind spot monitoring, lots of LED side lights. You turn those on with the cargo lamps, directional signals, running lights, all that good stuff on those mirrors.  Laramie stitched into the backrest. You get the gray piping. Both these seats are heated and cooled. Driver side lumbar, factory all-weather floor mats, auto headlamps, power pedals, and tilt steering wheel. Power windows, power locks, power mirrors. These mirrors also power fold in. And I always like showing that both sides are working, so that you know all the options are going to be working when you get here. The other thing that's cool about these mirrors is they have power blind spot mirrors. So if you look at that mirror on the left there, you can see that it is moving. And how you get that to work, you got to press the blind spot mirror button first, and then which side you want to control, and then that controls that. Memory driver seat. As we hop inside here, we'll take a look, turn on the ignition here. So you can see everything. This one has 3,910 miles. You get the seven inch LCD display. You can change those corners to read whatever you want, miles per gallon, time, mileage, everything, miles to empty. It's all configurable, so really cool stuff there. You get the heated leather wrap steering wheel, cruise controls on the right, gear selector on the right, Bluetooth and information center controls on the left. Being the diesel, it still has the six speed automatic transmission. And this one has the 12 inch Uconnect radio, AM, FM, Sirius XM radio capabilities. This one has the Alpine sound system. You also of course get the factory navigation system here. You can check out the backup camera at any time. You can also zoom in. Over the receiver hitch to get hooked up to a trailer, boat, camper, whatever you're towing the first time every time. You can control the fan and audio controls on the side here, or you can do the climate control on there. You also have your heated and cooled seat buttons that you can do, which show up there as well. And you also have all your different apps you can do, including the projection manager, where you can project your cell phone to the screen. So we actually did a demo on Android Auto. If you want to check that out, it's in the upper right hand part of the screen, but basically you can project your cell phone to the screen, control it through the radio. And if you've got different navigation systems such as Waze or Google Maps on either your Android or Apple device, you can project it to the screen and then use it through there. So pretty cool system. Check that out once again. Push button, four-wheel drive, factory brake controller. You get six auxiliary up-fit buttons for lights or plows or whatever you want to put on the truck. Toe haul mode, factory exhaust brake, front and rear parking sensors. You get two USBs, two USB-C's and an aux jack. And down there you get a 115-volt 400-watt plug-in. Keyless entry, remote start, power drop-down tailgate. You get two cup holders, coin slots and a nice little storage bin right there. And once again, this storage bin slides, which is a nice feature. Passengers side floor mat and seat are in excellent condition. Smells very clean inside this truck. I don't think it's ever been smoked in. You get speakers in the ceiling, part of that alpine sound system. Up here, your assist and SOS buttons, your map lights, your power sign, your window controls, and your power drop-down tailgate. You also have home link buttons there for your garage door security systems and lighting systems. And we'll take a quick look at the back seats. We'll start it up and then check out under the hood. The back seats are just as clean as the front seats. No rips or tears back here. It does have the latch child safety system for any child car seats you may have. Power sign rear window with the built-in rear defrost and seats fold up for extra storage.  If you've got pets, you can put both sides down. They've got a nice flat place to lay. We also have carpet floor mats that go along with the all-weathers here. And you do get storage underneath those seats. But if you've got pets, you can put both sides down. They've got a nice flat place to lay. Or if you've got muddy boots or tools, put them on there and you're not going to get your nice leather seats dirty. Back storage area, we have, sorry about that, removable containers for easy cleaning. And you can put ice and drinks in there. It's like you've got too many coolers wherever you go. And lastly, we have two USBs, two USB-Cs, and another 115 volt 400 watt plug-in back here. Child safety locks on the back doors. And we'll start it up and take a quick look under the hood. Only 3,900 miles on this truck. It has all the remaining factory warranty, bumper to bumper, and powertrain. Under the hood, we have the 6.7 liter Cummins diesel engine base. Very clean, runs very smooth. Once again, this truck has been fully safety-to-hand inspected by our server staff as a fresh oil and filter change. All the fluids have been checked and topped off. And this truck has 100%. Ready to go. And check out those LED headlamps there and fog lights. I would highly recommend this truck from a quality and condition standpoint. And to see more pictures of this truck or one of our other 450 new and nearest cars, trucks, SUVs, minivans, Wranglers, half-tons, three-quarter-tons, one-tons, you name it. We got it on our website right there at www.summitauto.com. Full pictures and descriptions of every single vehicle from two locations all at summitauto.com. And if you'd like to check out more HD videos, you can go to youtube.com slash summit auto. Remember to like, subscribe, and share on this video and all the videos that you see there. In fact, in a second you will see a link to subscribe to our YouTube channel on your left. It links more Ram 2500 truck videos like this one on your right. If you've not been to our website on the bottom link to this vehicle on our website, click those, check us out. And we really look forward to helping with this Super Clean 2020 Ram 2500 Crew Cab Short Box Laramy Night Edition in Delmonico Red Pro Code. Thanks again for checking out the video.


Hi everyone, my name is Neely and in this video I'm going to cover Canada's top drop shipping suppliers, eh? Canada's e-commerce industry is on the rise and reached a record $29.9 billion in 2021, making it without a doubt one of the more profitable markets to jump into. But in order to really succeed, you'll need to team up with the best suppliers that can handle Canada's high demand. With that being said, let's get to our list. Number one, Amazon Canada. I think by now, Amazon doesn't need any introduction. And if you don't know what Amazon is, I can only assume you've been trapped in a block of ice for 70 years like Captain America. There's no other explanation. Here are Amazon's best features that are definitely a worthwhile investment if you choose to use them as your supplier. They have multiple warehouses in Canada, which means fast shipping, typically getting packages to your buyers within one to two days. Quick tip, delivery time plays a significant role for drop shippers because customers want their orders fast. Faster delivery options can actually lead to more sales. Next, they have an endless range of products with millions of choices within any niche you can think of. If you wanted to sell socks for dogs, you could probably find a whole bunch of them on Amazon. They also have reliable customer service. And lastly, they offer super easy 30-day returns. Next on our list is Printful. Printful is a niche drop shipping supplier who specializes in print-on-demand products, which is a great niche to dive into just because there isn't too much competition yet. They offer print-on-demand customization for a lot of products like pads, shirts, laptop cases, mugs, and much more that you can customize per your buyer's request. Next, there's no minimum order needed to make a purchase from them, so you don't need to worry about ordering anything in bulk. They also have fast shipping. Ontario customers can expect deliveries within two days and the other Canadian regions within four days. Additionally, the Canadian dollar is offered as a payment option, so that means if you're Canadian, you can avoid those annoying conversion fees when using a foreign currency. Quick tip, the goal of this video isn't for you to choose just one supplier from this list. You can actually benefit a lot by working with multiple suppliers, whether it's for having a larger variety of products or just having suppliers as backups in case something goes wrong with one of them. Our next supplier is CJ Dropshipping. They're a leading Chinese supplier from China, but don't worry, they also have warehouses in Canada, so you don't have to compromise on shipping times. That's just one of the many benefits they offer. They also have a large variety of products with over 400,000 options to choose from. They offer print-on-demand services, which, like we mentioned before, means you can list custom designed products. They've got white label branding, so you can customize your brand packaging and increase brand awareness. They also share their own high-quality product videos and images, which can help you take your marketing to the next level. And lastly, they have free product sourcing. So if you want to list a product but you don't see it on their site, you can send a request and they quote you a price within 24 hours. Number four on our list is Etsy, which is an online site where you can shop for handcrafted products. Here are their top benefits. You have endless niches such as home supplies, furniture, jewelry, toys, and much more. They offer fast delivery, typically two to three days, because next-day delivery isn't always possible if you're buying something that's custom made. And the best perk is the potential for high-profit margins. Quick tip, anything that's handmade or handcrafted has a higher price tag, which shoppers are aware of, so you'll have the opportunity to set higher profit margins and earn a bit more with these kind of products. Next on our list is Wayfair Canada. This platform specializes in the home niche and was created by drop shippers. So as a result, they offer many drop shipping-friendly perks like a massive selection of products. Their selection contains over 14 million different products to choose from. They also understand the need for fast and free shipping for orders over $50, but in case you don't always spend $50 on every order and you don't want to pay that shipping fee, they have something called the My Way program, which is a $35 yearly membership that grants you free shipping for almost all products. Lastly, they support PayPal, which is known to be a fast and smooth payment method. Our next supplier is Overstock. Like Wayfair, this platform also focuses mainly on the home niche, which is one of the more popular niches. Here are the top reasons you'd want to use them. Most of the products have fast and free shipping since they have Canadian warehouses. Overstock also has a reliable 30-day return policy and sometimes even when you pass the 30 days, they'll still offer you a partial refund. They have a price match guarantee, so they want you always to get the fairest price. So they'll even refund you if you find an exact product somewhere else at a lower price. Overstock has a Club Zero program, which is their loyalty program that allows you to get certain perks like free shipping and 5% cashback on all purchases. All you need to do is pay the yearly fee, which is $20. And lastly, their customer support is really great. Quick tip, all the suppliers mentioned in this video are compatible with AutoDS, so if you want to implement any automation tools while using these suppliers, you can.  Our last supplier in this video is Costway. But if you want to see our full list of the top 17 Canadian dropshipping suppliers, make sure to check out our full blog by clicking the link in the description. Costway is a top dropshipping supplier that has high quality products and competitive prices. First, you can earn higher profits because of their coupons, deals, and 3% refunds. Another perk is their 90-day return and refund period. Costway also offers free shipping and they have over 8,000 self-branded products. Lastly, Costway offers a dropship certification and this allows dropshippers to register and obtain approval to dropship with Costway. There we have it. In this video, we covered seven of the top Canadian suppliers. But if you want to know our full list of 17 Canadian dropshipping suppliers, check out our blog. Link will be in the description below. If you learned something from this video, make sure to give this video a like. Thank you guys for watching and I'll see you next time.


The most brilliant concept in all of Chinese medicine is something called the energy dynamic, or Qi-Ji. And the energy or Qi-Dynamic is a concept that broadly describes how all of the factors inside of a person are contributing to health or illness. So the Qi-Dynamic is something that includes your diet, it includes your temperament, your character, your predominant emotional patterns. It includes emotions, it includes including external weather, how much you sleep. All these other factors are built into one concept called the energy dynamic. And the highest level of physician, all our ancient techs, some medical techs, the best physicians all talk about understanding the energy dynamic as the most important aspect of diagnosis and treatment before you do anything with acupuncture or formulas. So in this video, I'm going to introduce you to this concept called the energy dynamic, what it is, how it works, and why it's so important. Hey, Alex Hein, author of the book Master of the Day and current doctoral student in traditional or classical Chinese medicine. Now I've included the first link in the description there is a free download, little infographic, on five daily practices you can do to add 10 years to your life with traditional Chinese medicine. So you can check that out the first link in the description. So this idea of the energy dynamic is just a concept. It's something that encompasses biochemistry, hormones, sleep, emotions, and your unique constitution. So for example, someone who already has problems with digestion or bloating, that's showing a piece of the Chi dynamic, the energy dynamic that we need to take care of in our diagnosis and treatment. But then you throw in someone who also can't sleep and has anxiety, and it's something different. And we have to look at the big picture. What's going wrong with the energy dynamic in terms of directions. So it's a lot like physics where we need to see what is the direction of treatment first. Directionality comes before the formula and the acupuncture. So let's go over the case of a very common modern patient. The person has reflux, insomnia, and anxiety. So insomnia is a state where the person cannot fall into sleep. The ability of the body to just lay down, withdraw in and pass out is not working anymore. There's dysfunction. Acid reflux is called stomach counterflow. So the energy of the stomach naturally wants to go down. If it goes up, burping, hiccuping, nausea and ultimately vomiting. That's the most extreme stomach counterflow. And with anxiety, the person's kind of like this, like vibrating on edge. Like the nervous system is just like whacked out. Like, where's the threat? Where's the threat? Where's the threat? You know, it's really on edge. Now, that is obviously not a normal state. The healthy, well rested, well balanced person is calm. They're not easily rattled. They're not easily upset. They're not easily offset. So anxiety is a failure of this, this like the warm blanket reflex. Mommy puts a blanket on you in the middle of winter and you just kind of settle in. That, in the sense of anxiety, where the Qi dynamic is malfunctioning, the energy dynamic, is that it's all going out here and it's supposed to be coming in here. So it's really helpful to think of things in terms of forces like physics, like a vector. There's a direction and there's a force, right? Force with a direction.  So the big picture in this particular patient is there's too much going up and out. And we want it to go down and in. So that's what we want the acupuncture and the actual herbal formulas to do, down and in. So why do we treat this energy dynamic? The point is that number one, this is understanding the essence, the behind the scenes energetic or non-material pattern that creates the physical symptoms. This is really considered treating the root according to Chinese medicine. And all of Chinese medical theory is based on this first, not on treating symptoms or treating diseases. So the beauty of this is that not only can you treat something before you even know what it is, you can have anything walk in your door. And if you're skilled at spotting the energy dynamic, you can treat it. You don't even have to know what it is or how it happened or where it came from, you can treat it because the symptoms, the pulse, the tongue, the channel palpation and the symptoms all indicate where the energy dynamic is going. Usually it's going too much up and too much out and not enough storage and downward in modern people. So you can get started even treating complex cases and understanding what is the root before treating it if you know the energy dynamic. But most practitioners are stuck with, if they don't know that, just using a pill for a symptom much like an allopathic physician would. So that's an intro to the energy dynamic or Qi dynamic, one of the most important concepts in becoming a skilled practitioner of classical Chinese medicine and understanding what it is and how it works. Now again, in the first link in the description I've included the free guide on how to add 10 years to your life with five daily practices, so you can check that out in the first link in the description. And you can also check out my last two videos right there and right there.


Test his test his bundas bundas Liga Liga one two three Now then I'm not gonna fart about I'm just gonna get straight into it because as the title may suggest We're here to nerd out about this bag, and that's all we're here for we're gonna put our nerd gigs on And we're gonna delve into this bag mate.  It's an absolute beast of a thing and a few people have asked me if I would do a review on this So I'm doing it. I'm doing it I've used it enough now to do a comprehensive review and give my honest opinion on it And also I just wanted to run through a few features that if you own this bag you might not know about so Let's get into it So we're gonna start off. I mean the materials and everything it's got it's It is nylon the lead, but it's got it's in There's thicker material on the bottom for when you're putting it down I'll put a link below for this particular bag. So if you want to buy it or check out the The weights and the materials and everything you can have a look at that I'm just going to run through a few feature features of it and let you have a little look at the Straps and the internals and stuff like that. So what makes this a Quality bag is this the anti-gravity system on the back Which is basically? It feels like it's floating when it's on you. It takes all the weight into the hips and these hips are made From a material which will eventually with the heat of your body It'll adhere to your to your your shape So if it's uncomfortable at first or if it's rubbing just bear with it It will it will conform to your shape I believe the Osprey have like a heating thing where they will heat the Heat the pads and you can sit it on you and it'll it'll do it a lot quicker But just give it time and it will adhere to your shape It the anti-gravity system It is done by Velcro. There's a huge Velcro pad there which you can then You can lift it up and down and then put it back on and use these straps here To lift or lower each side. It's just a case of putting it on your back Jiggling around with it and having a little play to see what's what suits you what's most comfortable for you and It keeps the whole thing keeps it off your back It keeps that you see your back's not sweating. It's got this mesh which extends to the hip as well If you can see when that's pulled out this hip. I mean, it's so tight Yeah, you don't even need to fasten it at times. It's just just sits perfectly tight You can just carry a lot of weight in it comfortably and the reason I've got this bag It was through winter and whatnot like I normally use my 48 liter, but 70 litre is when I'm taking all my camera equipment You know food and you a bigger tent you want to just have a little bit more of a luxurious camp and you're not doing Multi-day hikes and this is the perfect bag Perfect bag for a one or two nights or long distance hiking in in colder colder weather. I'd say so many compression straps So many buckles so many things there. I love it. I'm a complete nerd when it comes to bags And I think I've got three or four off sprays I'll just love them. I think they're brilliant. So these side you've got these side pockets and each you've got compression straps, but You can choose to have your compression straps in tight to the bag or they can go over the pocket So I'll put I put one In and then one out. So if I want anything that needs securing there, it can go there And this one's just for water bottles. So the straps don't get in the way But you can mix it up However, you like it's easy access. So the front straps the compression straps you get you open them up and then you can You can get in the front Yeah, so you can get in the front then you can you know if you need to get your stove out or Something from the bottom. You're not having to take everything out the top of your bag Really handy that really handy and also in the same vein. There is a At the bottom So you can get in from the top the middle and you can also get in from the bottom as well And this is where you put your sleeping bag Instant access. There's even a divider there which you can it's optional. You can take that out if you want But it just it keeps your sleeping bag away from everything else So if you've got wet clothes or anything that you just want to keep away from the rest of your kit That goes straight in the bottom there. It's really easy to access and again, it's got compression straps on the bottom But these come really far out so you can use it to strap your tent on to or a sleeping mat Whatever I'll tell you what mate. I'll show you this. This is a really good feature. So if you've got a little a Osprey bag with the little with the buckles on You'll find in here Behind the compression strap of these little loops so you can and there so you can clip on your mini Osprey now I've had this one for 15 years this one the Osprey Viper 9 So you see that turns it into like you can attach that if you're going traveling or If you're going for long multi-day hike So you're going to a campsite where you want to do little day trips away from from camp then that attaches perfectly You can even put the big compression compression straps over it as well And then you've got yourself a built-in the ultimate built-in day pack Which I thought was a really cool feature it's like You know when you know, we're not transformers get together It's like that or whatever they call power rangers or whatever they called Wombles way below anyway, I love that feature. I think it's great Let's put it on on the straps itself.  Just if you're lying on your back. You can just go Help me It's a really good feature because you can't always reach your whistle. So it's always there for you I think that's excellent Yeah, well, I've got it on my back back. I'll show you the So comfortable make look at it. It's quite easy for your hat for your walking poles There's a little loop down here and then Into the top bit Tighten it There you are and they don't get in your way and they're always there It's a great design. That saw me across the country that in my osprey for yeah, I think it's a great It's a great little feature You'll see you might not know that these race up tucks up into here if you get it out you've got these Loops there's one on each side. I won't get that other one out, but there's one on each side And that's for your isax and then it just the loop just tooks back up into the bag itself Look into that little space there just keeps everything nice and out of the way, which I like These compression straps that go across here as well. They also once the bag is empty They will stretch all the way around Because you've got these side compression straps You can undo this and that can stretch all the way around to this one here and compress the bag Completely on your back if you're walking around and you've got nothing in the bag And you want the ultimate compression and that'll do that for you. Yeah, it's You can fit a bladder in it as well Which comes out here and you've got the option then to go down either one of these either one of these straps Also on the belt on the actual on the actual hip itself You've got these two huge pockets and you can fit your phone Trail mix snacks Whatever you want into them mate. There's loads of room in there so you've got two Two pockets at the top a huge pocket. This is where I keep my first aid kit knife gloves You can even put a little tap in there anything that you need to get to quickly and then in this one you have the brightly colored Raincoat and it's got a little toggle That goes through this loop here So that on a windy and rainy day, it's not gonna It's not gonna blow away And I believe that So you get that with the Europe. I think it is European model comes with the the rain cover Don't quote me now. I think in the US model. I think the the top bit turns into a day pack but again this top bit can be used separately You have these little buckles So easy to do it's just clip clip done I'll take this off now He says it's easy, but look he's struggling there you go. It's really easy just to detach the bucket top there and then what I do is If you just it doesn't mean it doesn't turn itself into a bag that you can carry say, but if you take one of these to the to the other corner and Same with the other one you can just chuck it over your shoulder like that and just Be hands-free with it And if you just at camp and you want to might want to go and watch the Sun go down You want to put a couple of cans in there and a Whatever you want and that's a nice little feature to have if you need it and then Underneath there if you don't have that on Hidden under if you turn these inside out you'll find the female side of these buckles so you can You can use that as a as a lid Really good really simple system to get into even if you've got your big big gloves on A lot of room mate absolutely just acres of it. You've got compression steps inside. Oh, hello There's no other pockets in there There's just the back bit which has got a pocket down the back which has sometimes slide me grilling and Easy just to put back in there. So yeah, that's all of it really and then sir and then at the front Once that's got you've got this big stash pouch here Big mesh it's a mixture of different material. It's got the mesh and the whatever nylon it is And then you can put your wet clothes in there or just stack bits and pieces really quick while you're on the move That's it mate. That's it mate Just play around with it with the anti-gravity system just play around with it until it until it Until you get it right now. I've taken it on a good few adventures So I felt like it was a good time just to let you have a little look at it All the features and stuff and you know, even if you do own this bag It's maybe good for you to have a look at stuff that you might not have known or Wondering what that buckle does or what that strap does If you have any questions about it, just chuck them down. I'll try and answer mate. It's hard. I do find it hard to reply But to all of them, but I'm sure in fact if you put if you have if you do have questions put them down there because there's a there's a high Brain in there and between us all about we know everything We are legion. We're stronger together So if you do have any questions chuck them down below and someone'll answer it. I'm sure Is that everything I've been through? Yeah, that's it mate and that's it The Osprey 8th of 70 AG and it comes in medium or large. I believe this is the medium but Go Osprey have got an app or go on the website and you can get measured up for it and see which one that you Would be best for you How long have I been going all right, okay? Well, I'll probably edit some out mate I can't wait to get back up in the mountains and start using this bad boy I'll leave a link below if you want to have a look at it or if you want to purchase it I'll leave a link below So you can do that right thanks for watching take care of yourself. Bye for now


Heather, you typically do a really good job on the base pass. What happened there in extras? Yeah. When I checked the outfielders to see where Conforter was, I knew where he was at. And I just tried to be aggressive on a ball where I thought was hit a little bit softer. And I got to do a better job of not turning my back to that ball right there and being too aggressive in a situation like that. That can't happen. But yeah, I just I tried to be too aggressive right there. Sting a little bit more happening in that situation in extra innings. Say that one more time? Does it sting a little bit more to have that happen in extra innings in that situation? Yeah, it's because we've grinded so hard. I know that's a guy we can steal a base off of too. But like I said, that can't happen. And I got to do a better job. Thank you.


This study shows that increased access to antiretroviral therapy has led to improved life expectancy among HIV-positive patients in Rwanda. Patients who were diagnosed earlier and initiated treatment sooner experienced the greatest gains in life expectancy. This article was authored by Sabin in Zanzimana, M.D., Eric Remera, Master of Science, Steve Cantiz, Master of Science, and others.


Okay, we're back live here inside Oracle OpenWorld 2012, day two, this is SiliconANGLE's exclusive coverage. Inside the trench is on the show floor, inside the booth here at Q-Logic, who's sponsoring us here, great support for Q-Logic. This is the Q-Logic Cube this week, because they're giving us their booth. Want to shout out to Q-Logic, day two is underway. I'm John Furrier, the founder of SiliconANGLE.com and joined this week by Dave Vellante, founder of wikibon.org, top analyst firm, open research, new way of doing research, wikibon is powering the way and exciting for day two. Dave, so day two we're starting, getting kicked off, got a big set of interviews, but the day started off with a real kind of somewhat quiet bang. Joe Tucci took the stage, some were discussing whether Joe Tucci would be punching back, but Jeremy Burton took the punch for him. So what's your assessment of Joe Tucci's keynote Jeremy Burton, the EVP of Price and Marketing? Well John, I got an email this morning from one of our followers saying, she's kind of surprised EMC has a keynote at Oracle Open World, given that they're really not good friends with Oracle, and I laughed and explained to him, look, EMC has to be here. They have to pay a million dollars for the right to address the 45,000 Oracle customers that are in the audience. And I thought Joe Tucci was very respectful, but he and Jeremy Burton, I thought, put together a good counter to Ellison. They didn't counter punch with regard to being in your face, but what they did is they talked about their strategy and their vision in a very crisp and credible way, talking about their cloud vision, their cloud trust, transformation, big data, you know the typical EMC that we know well, because we've been covering them that well, but the traditional Oracle customer may not know as well. The one jab that Jeremy Burton did take was he said that you haven't heard much about openness and flexibility this week, or flexibility and choice this week. So that was a jab at Oracle, but other than that, they were pretty respectful and I thought did a very good job. You know, what's striking about Joe Tucci and comparing and contrasting him to Larry Ellison, both tech titans, captains of industry, you really see the contrast. You know, you go back to 2009 when Larry Ellison went on a rant at the Churchill Club about cloud computing. In a way, he was right at that time, but now he's fully on cloud, and he's just punching hard. He's a competitor, and we know all Larry, but Joe Tucci comes out as a statesman. He really showed class. Joe Tucci stood tall, and you know what? He did not want to go slinging the mud with Ellison, so I thought that was a cool move, but it's just a striking difference between Joe Tucci, Dave, and Larry Ellison. What was your perspective on that? Did you agree with that? Did you see the respect there, but yet the statesmanship of Joe Tucci? Yeah, I think it was absolutely the right move. It was, as I say, crisp and cogent and very credible. And I think, in a way, it was kind of funny watching Tucci geek out a bit. I mean, Tucci knows, he sees all these technologies, investing in all these companies. The guy's very conversant in the whole, the new trends that are coming on. He talked about the software-defined data center, which is their term, and VMware's term. We call it software-led infrastructure, a software-defined infrastructure, but he talked about what that is. He did a heavy dose of flash, which, of course, this audience will resonate with this audience because of the performance impacts of flash. So I thought he did a really good job. And I mean, Joe Tucci's in the driver's seat. You remember at VMworld, he was up on stage with essentially all his competitors, Michael Dell, Tom Jorgensen. He was like the godfather. Joe Tucci's the godfather of the IT industry. But he's excited. You can see the passion. I mean, his passion comes out in a couple of ways. One, we all know he's not retiring anytime soon, or who knows what that's going to happen. Well, that's what he told you on the mini-cube. Yeah, I got a little private one-on-one with Joe Tucci at EMCworld. But you see him on stage here, and I think at the end of his presentation was very notable. He basically said to the crowd, you folks are in the right place at the right time.  And this is a unique time in history. And he was truly passionate about the fact that this is a unique time in the business, in all the theaters, technology theater, the business theater, and also the market theater. So Joe Tucci essentially saying, go to work. And then Jeremy Burton came on to kind of mop up and take care of some of the specifics that they had to punch out on their messaging. I'll see the total sales job of the human face of big data. I mean, Jeremy Burton spent, I literally counted six minutes on that one piece, selling the EMC campaign, and then going into a bunch of examples. So EMC's all in on this face of big data. Let's see how that materializes. Personally, I think they could have used some better use cases. I'm not too sure. Great clips is a good example. I would have gone more specific around changing society and real business benefits rather than getting a better haircut and finding out where the taxi lines are. The big data examples I thought were kind of light. They were fun, but they weren't really big data. So we're going to ask Jeremy Burton who's going to be on there today at 12, 20 Pacific time. It's a first step though, David. First step in at least moving outside the geek world of, hey, I have a zillion clusters. I can boot this up on the cloud. We've seen that at Google IO, for example, with MapR, for example. So a lot of people go on speeds and feeds. Jeremy's taking that first baby step to the real world and bringing in the big data message and the Kool-Aid to the masses. So one, props on that for Jeremy Burton, big fan of the campaign. But you did kind of a sales job up there. So we're going to hit him up on that.  Now we've heard a lot this week about Flash for as long as computers have been around and disk drives have been around, disk drives have been the bottleneck because disk drives spin their electromechanical and everything else is running at silicon speeds. So as Flash permeates the IO stack, networking becomes the real key focal point. And that's why we've been hearing so much talk about software defined networking. And we've got some major emphasis this morning, John, with our guests on networking. We have Lee Doyle coming on. Lee Doyle for a long, long time, probably the better part of two decades was IDC's number one networking analyst. He spun out, he started his own firm. Doyle Research, he's coming on. He's going to give us his perspectives on the networking industry. We also have Q-Logic coming on. John, they have a project called Mount Rainier which sort of blends the networking fabric with Flash to allow people to actually share Flash at synchronous distance. So that's going to be kind of an interesting segment. We have two Oracle, previous Oracle executives, Jeremy Burton, obviously the EVP of products and marketing at EMC, ex-Oracle. Also we have D. Dre Penday from CEO founder Newtonix, another Oracle. And what's interesting about the Newtonix CEO, Dave and co-founder is that he actually worked on Exadata's business. He built that engineering team. So that's going to be a good segment. We also have, that is going to be a good segment. We also have Gary Ornstein coming on at noon, Pacific time. Now for those who don't know Gary Ornstein, he was a writer at GigaOM. He's now the VP of products at Fusion I.O. But more than that, Gary just knows the industry really well. He blends technology and business. He's always one of our best guests. And then we've got a segment on a topic that really hasn't got a lot of play thus far. Larry Ellison talked a little bit about it on Sunday night. Jeremy Burton touched upon it just in a little very brief way as that Joe Tucci, but data protection. Back in recovery as you recall, a couple years ago EMC paid $2.5 billion to acquire data domain. So we're going to hear from those guys and talk about data protection in the world of Oracle, which is obviously an important topic. We got other notable guests, the TenGen president, Max, who TenGen is a company that makes MongoDB. This is the hottest, the fastest rising analytics, database, full package, competes with Hadoop, and they're growing absolutely crazy right now. And very popular in the data scientist, Dave, our research has shown that MongoDB has surpassed Hadoop in terms of popularity, in terms with data scientists. But in the IT pros category, Hadoop is more popular than TenGen's MongoDB. So you're saying you're not running MongoDB with Hadoop? Is it an H-based competitor? Is it an H-based competitor? It's a whole different system, a different stack, but you're not running it on Hadoop? No, no. Okay, so it's another fork. No, it's a separate opportunity, right? Just like Cassandra, React, all these other databases. So Cassandra run on Hadoop, right? We have violin memory systems. We have Bill Schmarzo, the dean of Big Data coming on. He says CTO for EMC, he's out in the field, he's an architect, and he's talking to customers. So we're going to have a customer perspective this day. We're going to have some great EMC guests on. We're going to have some great Oracle architects on. Don't forget, we got Larry Ellison coming on. He actually won't be in the hot seat here, but we will pipe through his keynote. I just got a text that said he might be swinging by later, so watch SiliconANGLE.tv for Larry Ellison. He'll be here. We have a seat for him. And Larry, as soon as you get down here, let us know we're in the 1900 section of the floor, Moscone South, looking forward to having Larry Ellison on the cube on SiliconANGLE.tv. Yeah, so that'd be good. We'll definitely broadcast this keynote, have some commentary, and maybe you'll come by afterwards and talk to us about it. So his keynote starts at about 2.45, 2.30-ish this afternoon. We'll be... I think 3.30. 3.30, we'll be carrying highlights. And of course, epic commentary, independent analysis here with SiliconANGLE, Wikibon, live tweeting. We're going to have some, we're going to go up against Ray Wang's tweets. Like we always do, he's got a point of view. We have an angle. We're going to have fun with Ray and all the people on the Twitter stream. Really, well, that's where the action's done. That's where the crowd will be curating the commentary. We'll be there leading that. Also providing video commentary and breaking analysis exclusively here on SiliconANGLE, Wikibon. So buckle up for a great day in day two. This is theCUBE on SiliconANGLE.tv, SiliconANGLE.com, with Wikibon.org, providing the great analysis. This would not be possible without QLogic support and all of our sponsors who allow us to come here and do our great independent analysis. So we appreciate QLogic. Come to the QLogic booth, tell them that John sent you and you get a 10% discount on fiber channel cards. 20%. 20%. I think you go for 20. You got Papa John's ad? Yeah, 20% discount. Just say John Furrier sent me. I want a fiber channel card. I want some switches, FCOE. They have it all. QLogic's awesome. I want to thank QLogic. We'll be right back with great coverage here live at SiliconANGLE.tv's Oracle Open World from the show floor. Oh, look at this. The cube is this conceptual box, if you will.  The cube is a comfortable place. It's a place where people feel happy and are happy to share their knowledge with the world. And we're happy to be ambassadors of that knowledge transfer.


Governor Kefas Aghu has flagged off the distribution of over 100 million Naira to some 10,078 vulnerable people across the 16 local governments of the state. Flying off the distribution of livelihood grants to the Tarabah State COVID-19 action, recovery and economic stimulus, the governor said the project is targeted to support youth empowerment as well as to reduce poverty. Governor Kefas, who was represented by his deputy Aminu Ablahi Akali, presented the beneficiaries with their various checks. The Tarabah State COVID-19 action, recovery and economic stimulus, the stimulus program is an emergency operation designed to support the program of expenditures and interventions at the state level. Targeting existing and newly-inmatched vulnerable and poor households. I look at how value chains and micro and small enterprises are affected by the economic crisis. I feel happy and I feel exited with what the Tarabah State government in conjunction with the World Bank has been giving to me as beneficiary in this program. I really appreciate and I really exited and I'm very happy for the program. Hopefully the program is holding now and I will make sure that I will use the money to just share it for the work and I will make sure that I will employ some youth under me so that they will benefit. We don't want for this day that people know Tarabah State will be happiness to us and we will do some things that will make you happy for tomorrow and we too will be happy for this program. May the Lord provide the many ones for you and us too. Thank you very much for watching.