Welcome back everyone. Let's just begin with the word of prayer. Would someone open us in prayer please? Let us pray. Heavenly Father we just wish to thank you for this hour of study and we pray that whatever we study today Father we will be able to apply it in our lives and Father we also pray for a blessing upon our entire faculty and all the students here Father. In Jesus's precious name we pray. Amen. Amen. Thank you. Okay so we'll just quickly look at what we covered, a little bit of what we covered last week. So we did the outline of the book of Matthew. I won't go into the outline again. And then after we looked at a few specific topics one was the virgin birth. So seeing how the virgin birth is clearly talked about in scripture and that is a fulfillment of what was prophesied about in the Old Testament. We looked at what repentance means. So when Jesus, when John the Baptist are preaching about repentance, what does that repentance actually mean? And then kingdom of heaven, what does the kingdom of heaven mean? We then looked at the Lord's prayer specifically in relation to the kingdom of God and how we see that prayer impact how we live within God's kingdom. And then we looked at a few specific topics that are in the book of Matthew but also in the rest of the New Testament and what we learn from the New Testament about these things. We looked at miracles and we were looking at these topics and the relevance for today for believers today. So we looked at miracles, we looked at demons and then we came to the parables of the kingdom. So this is something that I had asked you to just read through so that since we are also trying to catch up on content, I'd asked you all to do this at home. To read this Matthew 13 3 to 50 and to note down some things that you all noticed about what was the expectations of the coming kingdom versus what Jesus was teaching. So were you all able to do that? Okay, we'll just quickly go over the notes. I do want us to be going back to scripture and not only, so we don't want to just look at notes on the New Testament, right? We want to be actually reading the New Testament through this class. So for us as much as possible to be reading directly from the word. So that's why I'd asked you all to go back and read Matthew 13. If you are able to read through the whole New Testament through this class, just read through the book. Before we discuss it in class, that would be that would be really, really good for your own personal understanding of what all we're talking about in class. But yes, as much as possible, we'll try and for each of you to go back and read parts of what we're talking about in class, so that it's not just a discussion based on the word without actually reading. Okay, so we don't have time to read through the whole chapter. We'll just look at some things that are there in your textbook on the parables. So we see the parable of the Sower and here one of the expectations was that the Messiah would come and all of Israel and all the nations would turn to him. Whereas what is taught in the parable is that each person will respond to the word differently. So the word is sown and based on the person's heart, they will either turn to God or they will turn away from them. They will reject the message that is given. And so that final coming of the Messiah and establishing the Kingdom is something that we look forward to. But in the Kingdom that Jesus had brought, the Kingdom that was initiated through Jesus's coming, it was only those who responded to Jesus who would enter into his Kingdom. The King of the wheat and the tares, so this is where the wheat and tares are separated at the end in the final judgment.  But here we see that we continue to be part of the world as people who are part of the Kingdom were continuing to be part of the world until that final harvest. That's when we will rule and reign. We also rule in this presentation in fact that we share the authority that Jesus has given us to use his name and to exercise his authority here on earth. But at the same time we are still very much in this world. We are living with the same challenges that the people around us face. And until that final harvest, the separation between us and the world will not happen. The third is the parable of the mustard seed where the small seed is sown and it grows into a large tree. Here the expectation was that the Kingdom would come in glory. That's what the Jews expected. But the reality was that the Kingdom came in a very insignificant and unexpected way. But it's over time that the greatness was realized through Jesus' death and resurrection. And that glory will only continue to grow until the final establishing of the Kingdom of God. The parable of the leaven, this is talking about yeast being mixed into the door. The expectation was that only those who are righteous will enter into the Kingdom of God. There is no place for the unrighteous, which is why the Pharisees had the sense of self-righteousness. They viewed everyone else as sinners, as people who were outside. But the reality is that the Kingdom comes to everyone. The truth of the Kingdom, everyone is invited into the Kingdom. And the message of the Gospel brings that transformation, enables us to be righteous. So the righteousness that we have comes from God Himself, comes from the message of the Kingdom, which is the Gospel. The hidden treasure, the parable of the hidden treasure, which is somebody who finds hidden treasure in the field and then hides it, goes and sells everything they have and then comes back to take it. The expectation was that the Kingdom would come in a way that everybody would see it. Everyone would know that the Kingdom has come. But the reality was that the Kingdom came in a way that was hidden. Like we see in these parables itself, that it was presented in a way that was not fully revealed. All of the understanding of the truth of the Kingdom was not revealed at that time. But only those who really wanted to know the truth of the Kingdom who wanted to pursue it were the ones who could receive that treasure. And so it's a Kingdom that is hidden but is available for those who are willing to go after it. And then the parable of the priceless pearl, which is where a merchant finds a pearl that is greater than anything of greater value than anything else he has. He sells everything and comes back for this pearl. So the expectation was that the Kingdom would bring things of value to people. But the reality is that the Kingdom demands people to give up everything else to abandon all things that they value in order to receive this Kingdom fully. And so that was the difference in the expectation between what Jesus was teaching. And then the last one is the parable of, I believe this is the one with the fish. Let me just go back and make sure that I'm saying that I think 1347 to 1550. Yeah, so this is where a fish is cast into the sea and draws up all kinds of fish and then they are separated. They are only separated at a later time after all the fish are caught. So the expectation was that the righteous and unrighteous are separated right at the beginning when the Kingdom comes. But what Jesus was teaching was that it's only at the final judgment that the unrighteous will be separated from the righteous until that time the Kingdom will continue in this world. And it will be open to anyone, anyone who is willing to receive the message of the Kingdom. So Jesus spoke all of these parables in a way that was hidden. He didn't reveal all of the truth of this, of the Kingdom to people, but to his disciples he did. We know that he chose a few people who would understand or to whom he would explain the meaning of all of these things. And so we have the privilege of now reading back and knowing the meaning of the parables that Jesus taught. So with that we come to some of the closing things in the book of Matthew. So Matthew talks about the end times, about the final judgment, about the end of all that is created and the final establishment of the Kingdom of God. And we see that Matthew 24 talks a lot about that. Like we talked about earlier, Matthew actually pays a lot more attention to the end times than the other Gospels. Matthew 24 actually refers back to prophecies in the Old Testament. We have a few references there. So Daniel 927, 1131 and 1211. So these are some passages that Matthew goes back to. So he references these passages in the things that Jesus is teaching about in his teaching on the end times. So there are various teachings on the end times in the New Testament. We see in Matthew 24 there is talk about tribulation and rapture. In 1 Thessalonians 4 there is the gathering of God's elect. In 2 Peter 310 it talks about all that is created going up in flames. So there is a complete destruction of what is created and a new creation which Revelation 21 talks about. And then Revelation 21 to 6 also talks about 1000 year period when Satan is bound. And so we have all of these different teachings on the end times. And they're not necessarily put in a systematic way. We see them in all these different books. And even within the books they're not telling us what's going to happen one after the other. So many times there are questions about the end times. There are different interpretations based on how people are reading these things. Whether the tribulation will happen before the rapture or after the rapture. There are different teachings on these things. And you all will have a class on the end times in your Bible college courses. If you all are continuing to study.  But here we're just talking about the fact that Matthew talks about the end times. And the rest of the New Testament talks about the end times. And the Old Testament also talks about the end times. But it's not always understood in the same way. And so what can we take away from the New Testament's teaching on the end times? One thing is that we have to look both at the Old Testament teaching and the New Testament teaching. And if we look at it, we'll see that there isn't any contradiction between those teachings. The New Testament depends on the Old Testament references. The Old Testament several times when talking about the end times. And when we look at both together, we get a fuller picture of what scripture's teaching is on the end times. At the same time, it's not given in a systematic way. Giving us what's going to happen after what. And again, because of the use of pictorial language, because of a prophetic language that is used, there is that challenge in interpretation as well. We look at some of that in our interpreting scripture class. But one thing that is clear is that what we look at about the end times should impact how we live now. So we'll just look at these two verses. 1 Thessalonians 4, 18 and 2 Peter 3, 11. If someone can read those verses for us or two people can read the verses. 1 Thessalonians 4, 18. Maybe we can read from 16 to 18. 1 Thessalonians 4 from 16 to 18. For the Lord himself will descend from heaven with a shout with the voice of an archangel and with the trumpet of God and the dead in Christ will rise first. Then we who are alive and remain shall be caught up together with them in the clouds to meet the Lord in the air. And thus we shall always be with the Lord, therefore comfort one another with these words. Thank you. So here we see in verse 18, we know what is to come. We know that we are going to be with the Lord and that we will spend eternity with Him. So how should we live now? We should encourage one another. We should remind one another of that future hope that we have. So in the face of the challenges that may come up in our lives now, we can be encouraged. We can have something that we are looking forward to, that we are hopeful about because we know of the future that lies ahead of us. And can we read 2 Peter 3, 11 please. 2 Peter 3, 11, therefore since all these things will be dissolved, what manner of persons ought you to be in holy conduct and godliness? You can go on to verse 12 as well I think, just to the end of that sentence. Sure, okay. I'll start from the top then. Therefore since all these things will be dissolved, what manner of persons ought you to be in holy conduct and godliness? Looking for and hastening the coming of the day of God because of which the heavens will be dissolved, being on fire and the elements will melt with fervent heat. Thank you. So this is a call to live in holiness, live godly lives because we are expecting that coming day of the Lord. So we prepare ourselves like those 10 virgins, the 5 virgins who were ready for the coming of the bright room and the 5 who were not ready. We should be like those virgins who are ready that we have kept ourselves holy. We have lived lives in line with God's will, waiting, anticipating His coming. And then the New Testament also shows us this picture of all of history ending as God has intended it to end. Like God is on the throne, God is the God of all time, right? And so all of time is in His hands. What happens at the end is all in His will and in His hands and He will fulfill all of those things. So having that kind of hope, like we've seen in the Old Testament, the hope was for the Messiah to come. In the midst of the suffering of the people of Israel, there was always the promise of the Messiah coming. And that was what they were looking forward to the day of the coming of the Messiah. Likewise here in the New Testament is in the midst of all that we face, whether it is persecution, danger to our lives, those kinds of things or other challenges that come up. We look forward to that future end in the presence of God, which is free from all of the suffering that we are experiencing in this present life. So that is the picture of the end times of the New Testament gives us. Matthew also writes about. And then the final thing that we look at is the resurrection of Jesus. Now, Matthew of course talks about the resurrection of Jesus, but in the whole of the New Testament, we see that the resurrection is given a very important place in the Gospel. If someone can read 1 Corinthians 15-17 for us. And if Christ is not risen, your faith is futile.  So we see that the resurrection is such a key part of our salvation. So if Christ has not been raised, then we are still in our sin. We are still guilty of our sin. But it is the fact that Christ overcame that he was victorious over sin and death, that we have a hope of being redeemed, that we share in that victory as well. And so the resurrection becomes a key player in the Gospel, in the story of salvation and in the message that has been preached to people. So also when we look at one of the big challenges that is presented to Christians nowadays is how can someone who is dead come back to life? That is something that is questioned from obviously people who are thinking from a natural perspective who are not believers and who say that is just unbelievable. That's something that we cannot accept. But we should recognize that if we are removing the resurrection from the picture of the Gospel, then we are removing this truth of victory, of salvation, of forgiveness from sin, from the message itself. And so it's a very, very important part that cannot be lost just because for someone thinking in a natural sense, it doesn't, it can't be proved or it can't be explained. And we look more at why we can so confidently claim Jesus is resurrected as we see later on in the other Gospels and in the rest of the New Testament. Let's also look at Romans 1.4. So what all does the New Testament talk about with regard to the resurrection? Spirit of holiness by resurrection from the dead Jesus Christ our Lord. Thank you. So we see in Romans 1.4, he was shown to be the Son of God when he was raised from the dead by the power of the Holy Spirit. We see here that the resurrection proves that Jesus is the Son of God. That's what the New Testament itself is saying. That it showed who he truly was, that he was who he said he was and who the Gospel writers were saying he is. Let's look at Acts 10, 39 to 43. Acts 10, 39 to 43. And we are witnesses of all things which he did both in the land of the Jews and in Jerusalem, whom they killed by hanging on a tree. Him God raised up on the third day and showed him openly not to all the people, but to witnesses chosen before by God, even to us, who ate and drank with him after he arose from the dead. And he commanded us to preach to the people and to testify that it is he who was ordained by God to be judge of the living and the dead. To him, all the prophets witness that through his name whoever believes in him will receive permission of sins. Thank you. So we see the resurrection from the dead and forgiveness of sins being connected here like we talked about earlier as well. It being key to the Gospel message of us being forgiven of our sins. But even here in this passage that we read, we saw the talk of witnesses to the resurrection, right? He didn't appear to the general public, but he appeared to people who were chosen to be witnesses.  So Luke here is saying that he himself was one of the witnesses. And that command to preach the Gospel came to those who witnessed his death and resurrection. So the resurrection of Jesus is well attested. It was not only the 12 disciples. So those are some of the things that are challenged is whether the disciples themselves just made up a story like the 11 disciples who remained after Judas' death. They came up with this story and they told people. But it was so many more people than those disciples, right? We see the women who were at the tomb, so Mary and other women who visited the tomb in Matthew 28. And then we see Peter himself. We see the disciples on the road to Emmaus. We see the group that was present on the day Jesus was resurrected. Jesus appears to them. We see Thomas feeling the wounds on Jesus' side, on his hands. The disciples on the seashore where Jesus pierced them as they are fishing in the sea. And then over 500 people who had gathered saw Jesus. So this is a very, very large group of people. And then we see James, the brother of Jesus, and then those who were at Jesus' ascension. A large number of witnesses to Jesus' resurrection. So it's not something that can be disputed on any grounds of lack of evidence. There were so many people who had seen it. And the Gospel writers then write the Gospels claiming Jesus' resurrection. And those Gospels go out to so many people who read it. If what they were saying was not true, then people could have easily come against them at that time saying Jesus was not resurrected. But because there were so many witnesses, people who knew that what was being said in these Gospels is true. It was accepted and believed by people. And it's these witnesses who were key to the Gospel spreading after that. But at the same time, while we talk about there being sufficient evidence for Jesus' resurrection, we don't say that people should come to faith in Christ just based on that evidence. It has to be based on faith, right? So even in the Gospels, when Jesus is doing his miracles, there is evidence of Jesus' power, his authority. He's proving what he's declaring about himself. But those who refused to believe in him continued to oppose his ministry and were the very people who came against him. Likewise, even as we proclaim the Gospel, as people come with challenges of things like how can somebody who's dead be raised, we shouldn't go into those discussions thinking that we are going to prove Jesus' resurrection and these people are going to believe. It may not happen because if they have resolved in their hearts to not believe, then whatever evidence is provided, they will not be convinced. So it does require faith. In addition to the evidence, there must be a willingness to believe or to receive that evidence and respond to it. And then the last thing is, what is the relevance for us today? We'll just look at these two verses, Hebrews 7, 24 and 25. Someone can read that for us. Hebrews 7, 24 and 25. But he holds his priesthood permanently because he continued forever. Consequently, he is able to save to the uttermost those who draw near to God through him. Since he always leaves to make intercession for them. Thank you. So our salvation and our continued walk with God is possible because of Jesus' resurrection. So we are completely saved because he intercedes on our behalf before the Father. And that continues to be true for us today. Salvation is possible for all those who come to Jesus because he is before the Father interceding for us. And we are able to experience that full and complete salvation from sins and the effects of that salvation in our everyday lives because of Jesus' intercession. And then let's read 1 John 3 2. 1 John 3 2. Beloved, now we are children of God and it has not yet been revealed what we shall be. But we know that when he is revealed, we shall be like him or we shall see him as he is. So that is our present hope. A present hope is that we ourselves will share in that resurrection that it is a resurrection that will transform our bodies and we will be with Christ. We will be like him. So these are two things that we look at as how is the resurrection relevant for us today, apart from the fact that the resurrection is a hope of salvation. So for those who are coming to faith, the resurrection is a very important part. But for those of us who are already believers, it continues to be relevant because Christ is interceding for us. And we have this hope of our resurrected body of being with Christ and being transformed to be like him. So we come to the end of the Gospel of Matthew and we'll just start a little bit. We look a little bit at the Gospel of Mark today and continue on Thursday. If you have the time and are able to read through the Gospel of Mark in the meantime, please do that. So we, like we said when we were starting the book of Matthew that Mark was actually, Mark is actually a condensed form of what is used in the book of Matthew and in the book of Luke. And is believed to be the source of what they talked about. So they depended heavily on what Mark had written. Some people think that there was a separate source and all three Gospels depend on it, but Mark was the earliest of the three Gospels. And initially Mark was neglected, was looked at as a Gospel that was not very important because people thought that Mark had just was just like a small somebody of what Matthew talked about. And because it was also mostly dependent on Peter's, what Peter had talked about Jesus. If we look at first Peter 513, maybe we can just read that one Peter 513. She who is at Babylon, who is likewise chosen sends you greetings and so does Mark my son. So we see that Mark was ministering with Peter and so a lot of what goes into the Gospel is based on Peter's recounting of Jesus and of Jesus's life and ministry. So because the book of Mark was mostly dependent on Peter's recollection of Peter's preaching and because it was such a small book, it was thought to be something that was not as important. We did mention this earlier that Matthew was a very popular Gospel being circulated in the churches and initially Mark was not. But it's only now in present day scholarship that as people began to recognize that Mark was the earliest Gospel written and Matthew and Luke depended on Mark's account that this Gospel has started to become or be considered more important. Now Mark is very action oriented. So when you read it, it's just going from one event to the other. There's not a lot of focus on Jesus's teaching or preaching. Like Matthew, Matthew has those five different sermons, large chunks of Jesus's teaching. Mark doesn't have that. He has much more focus on what Jesus did. And so he presents Jesus as this powerful person or we look more at what he says about Jesus, but who conquers demons, disease and death. This is Jesus, the Jesus he presents, someone who is active and someone who is doing certain things to prove who he is. We see straight away and immediately used a lot in Mark. So just an indication of that action oriented Gospel. It's very, very focused on what is happening next, what happens after that and how Jesus is moving from one place to another doing different things. Mark as opposed to Matthew is focused on the Gentiles. So Matthew was focused on Jewish Christians. Mark is focused on Roman Christians. And so the language that is used is much more suitable for those who are not from a Jewish background. We'll just look at these two passages, Matthew 10, 5 and 6 and Mark 6 and 7. Just as one example of how they look at the same, they narrate the same story in a different way. Matthew 10, 5 and 6. These 12 Jesus sent out, instructing them, go now here among the Gentiles and enter no town of this American. But go rather to the lost sheep of the house of Israel. Thank you and Mark 6, 7. Would someone read that please? Mark 6, 7. And he called the 12 to himself and began to send them out two by two and give them power over unclean spirits. He commanded them. Oh, sorry. Thank you. So we see Matthew specifically mentioning that the disciples were sent out only to the people of Israel. Whereas Mark doesn't include that in his in his recounting of the same story. So the way they presented based on their audience, based on their focus is different, right? This is what we talked about. Why are there four gospels? It was based on their audience. What did they want to highlight? So Matthew wanted to highlight Jesus' ministry to the people of Israel. Jesus had come for the Jews. And then that gospel was also then to be taken to the world. Whereas Mark is more interested in just presenting the gospel for all people because his audience is a non-Jewish audience. So it isn't relevant here to say that Jesus told them to go only to the Israelites. What he wants to say is Jesus sent them out to minister to people. And likewise we are sent out to minister to people as disciples of Jesus. So that's the difference between their audience and how they present the same story. Mark is also very, very honest. We see this actually throughout the gospels. I did mention this in one of our classes that the gospels are also the genre of ancient biography. So in ancient biography, they would retell the story of a hero. But the difference between the gospels and those biographies would be that those biographies would highlight only all of the positive things. They would never say anything negative about the hero. And they would also exaggerate a lot so they would make the hero look like a superhuman. Whereas we don't see that in the gospels and especially in the book of Mark. We see a very honest picture of the weaknesses of the people that are being talked about. Even though these were supposed to be the heroes of that day. These were the disciples who were leading the churches. They were the ones who had established all the churches and were now raising up new disciples. But they don't hesitate to present these disciples as weak. So we see in all of these passages in Mark, the disciples lack of understanding of Jesus' mission. Lack of understanding of his, when he talks about suffering. Even when they're seeing the miracles, they don't understand. So when they see the miracle of feeding the 5,000 of the lobes multiplying. They still are confused when Jesus says, don't share in the bread of the Pharisees and the Sadducees. So they think it's about physical bread. So this displays their lack of understanding. Even though they are with Jesus, they are hearing his teaching. They are seeing his miracles. They still don't understand his mission as Mark presents it through this book. We also see Jesus' relatives not recognizing Jesus' Messiahship. If we look at Mark 321, can someone read that for us please? Mark 321. But when his own people heard about this, they went out to lay hold of him for they said he's out of his mind. So we see within his family itself, the opposition that he faced that they didn't understand his ministry. So Mark isn't trying to hide that. And even though James later becomes the leader of the church in Jerusalem, Mark doesn't hesitate to present his own family, Jesus' family. James was one of them as people who viewed Jesus himself as out of his mind during his ministry. And then Mark presents Jesus as a very human person with different emotions. He doesn't hide that side of Jesus. We see in all of these verses, I don't think we have time to read them. But Jesus' sadness, his anger, his disappointment with people's lack of belief. All of those things being presented to us. Jesus is presented as a very human Messiah, not someone who is just divine. And we'll end with this. I think we have a minute. If we don't finish it, we'll continue on Thursday. So Jesus as the Son of God, Jesus as Son of Man, Jesus as Redeemer. If we look at Mark 11, we'll read these verses. Okay, let's just read Mark 11 and Mark 11. The beginning of the gospel of Jesus Christ, the Son of God. And a word came from heaven, you are my beloved Son, with you I am well pleased. So right here at the start of the gospel, Mark declares Jesus as the Son of God. So this is a very important part of who Jesus is that he wants to be made clear through the gospel. Mark 11 right at the start, Jesus, the good news about Jesus Messiah, the Son of God. And then in verse 11, the Father himself affirming who Jesus is. Jesus is my Son in whom I am well pleased. And then Mark 1539, we can read that. Mark 1539. So when the Centurion who stood opposite him saw that he cried out like this and breathed his last, he said truly this man was the Son of God. So beginning the gospel with this proclamation of who God is, who Jesus is, and then ending at the end of Jesus' crucifixion, this declaration by the Roman officer, the recognition of Jesus being the Son of God. Again, his focus on a Roman Christian audience and also the fact that this identity of Jesus as the Son of God is being established right from the start of the gospel to the end. So we'll end with this and we'll continue on Thursday. I'll just keep you notified if the class will be online or whether I'll be on campus. I'll post that on Classroom based on whether I'm able to come back in time for class on Thursday. But thank you all so much for being here. We'll see you on Thursday.


Welcome everyone to this tutorial on large genome assembly and polishing. Here is the tutorial material and while I'm showing this tutorial I'll have this open in a different window and I'll show you the steps that I'm following in the Galaxy window. So in this tutorial we're looking at what genome assembly is so I recommend reading the introductory material here if you're not familiar with some of these concepts. We're looking at some of the challenges in particularly assembling these large genomes back into what we are hypothesizing as their original chromosomes. So there are a lot of challenges here and we'll definitely see that with this test data set and you'll no doubt see that with any real data sets that you try to assemble as well and that's just part of the current landscape of genome assembly where we're still in the process of developing all of the tools and the backend computation to try and work with these huge volumes of data. You should be able to use your own real research data with the workflows described here. So we do always recommend though checking each tool separately on any data that you want to use and also perhaps sub-sampling your real data sets so that any problems you can find out more quickly rather than setting a big job running and having to wait a long time. And if there are any issues with the tools or the data running on those tools then contact the people who are administering your particular Galaxy server. Today I'll be showing these workflows running in the Galaxy Australia server and again if you are using your real data in with these workflows keep in mind that so many genomes have never been assembled before so it's likely there are going to be particular settings in the tools that you'll need to change or customise so that they're relevant for your input data as well as your research questions. So in an ideal world we would be able to make perfect assembly workflows that you could just put your data in and run but I think if you want to get the most out of your data and your research you really want to be thinking about what those tools doing and whether that's working well with your particular data type. So in this tutorial we're covering various steps. This is a picture of the analysis workflow that we'll be following so this involves quality control, counting the camers in the reads to get an idea about the genome characteristics, trimming and filtering reads, assembling and polishing the genome and then trying to assess the quality of our genome assembly. In this tutorial we'll actually run these as separate workflows and we may change some of the parameters in each of the workflows as we run them so rather than running all of the separate tools we'll actually be running a set of workflows. In this tutorial we talk about how you can run workflows in Galaxy and where you can import these workflows from. Okay so now we will start with importing the data so let's give our Galaxy history a name and then in the tutorial document there is a list of the files that we need to import so I'll copy that list then I'll go here into upload data, paste fetch data, paste those file names in there, start and close and these files will now start to load into the current history so now our files have imported and we just need to check that they're in the correct format so you'll notice you've got three eucalyptus files, they've got the sequencing reads in them and we need to check they're in the format fastq.gz if they're not in that format make sure you click on the pencil icon for each of these three eucalyptus files and assign the correct data type which is fastq.gz sometimes Galaxy will upload them as fastqsangr.gz but they need to be fastq for this tutorial. The next step is to check the read quality so for this step we're going to run our first workflow which is the data qc workflow and this is going to report some statistics from our sequencing reads so in the tutorial document it explains how we can import this workflow and I'll just show you that here so we can copy the link from the tutorial and then we want to import it into our own Galaxy set of workflows and then that should now appear in our list of workflows and your list of workflows will look different to mine but it should load as the top workflow and once that workflow has loaded to your list we can run this workflow with the run button and in this case I usually expand to the full workflow form just so I can see exactly what's happening here we won't send our results to a new history we're just going to do all of our workloads in the current history and we need to check our input files are correct so Galaxy will guess but we need to check so for long reads we want to make sure it's the nano file and nanopore reads so that's correct for our elimina reads we want to check that it's the r1 fastq reads and then for the elimina reads r2 so that's our paired elimina reads and in this particular workflow all of the parameters have already been set to particular defaults and you can always look at those by looking at the workflow either here or in the workflow canvas and you can change anything that you want to change so we won't look at all these settings currently but we will now run our workflow the workflow is now running we can see the workflow invitation and this describes the inputs and the outputs and the steps that are being run so as the workflow runs this will fill out so it shows us what inputs we used in this particular run for workflow what outputs we're hoping to get and then more detail about all of the steps that are being done in this workflow so to get a full idea of what's in here have a look in the tutorial document and also at the workflow itself and then you can see the tools that have been used in this workflow which in this case are the nanoplot tool and the fastqc tool and then combining those results with the multiqc tool and we can see our jobs are starting to run here in the history and when they they've finished running they'll turn green let's have a look at some of our results uh first we'll have a look at these multiqc results and we'll just collect some of our side panels so we can see a bit more clearly here this is going to summarize our laminar reads results from fastqc and for example one of the things we can look at is the sequence quality histograms and we can see that it reads quite good quality there's some drop-off in quality at the end but not very much at all and we could also look at our nanoplot results let's have a look at that html report and in particular let's have a look at read lengths versus average read quality and we can get lots of information there and so from these qc results you might then make further decisions about firstly whether your reads are of high enough quality for your particular analysis that you want to do and also if you need particular settings on any filtering or trimming of reads according to their quality now we will look at some of the genome characteristics and we'll do this by counting the camers in the sequencing reads and there's more about this explained in the tutorial document so what i've done is i've imported the workflow for this one already this is the camer counting workflow and we can look at that in our workflow list so i imported it the same way that we did in the previous step for the data qc workflow so here's our camer counting workflow and with all these workflows one way of looking at them in more detail is clicking the edit button and then you can view them in what you call the workflow canvas and this is just a nice way of seeing how all the tools and the inputs and outputs are connected together this one's not a particularly complicated one we'll collect our side panels here so this is a very simple one but it gives you the idea of how things are connected there's the input going into this particular tool and that tool's output is going into this tool and then these are all about outputs here from the actual workflow and this is another place where you can see all of the settings that have been put into the workflow and you can decide whether they're right for your data or if you want to change them so for example let's have a look at the marrow settings by clicking on that tool and it brings up the tool over here at the right and all of the settings that have been put in so these are some of the things that you may want to change for various reasons so exit back out that workflow canvas and again we've got our workflow here in the list and we are going to run that again just with the results going into our current history and all we need to do is check that we've got our correct input file here just the rrun file and click run we have our results now so one of the things we'll look at is the genome scope transform the linear plot with an eye icon just make a little bit smaller lock so this is a graph of the results of our came accounting and it will tell us some of the estimates it's made about the genome size and the ploy so this is discussed more in the tutorial but this is quite a useful graph as a result from our came accounting now we will run the step for trimming and filtering the reads so I've already imported this workflow and we can just run it here directly and we need to give it the correct inputs so check it's got r1 here r2 and the nanopore reads here and we can run that in the tutorial I discussed more a lot about what settings have been put in there for the defaults but note that you will most likely want to change some or all of those um existing settings if you're working on this with real data let's have a look at some of our results we'll have a look at the fast p results for the Illumina reads click here with the eye icon and we can just get a lot of information there about the results of fast p from both the trimming and the filtering that we did in this workflow and now we're up to the genome assembly stage so we're going to do that with a assembly tool called fly so again I've already imported this workflow you can see that here at the top of the workflow list so import that into your own uh list of workflows and then let's run that and we'll expand to the full workflow form so for this one we need to give it the input long reads and we'll give it the reads that went through the trimming and filtering step so these are the fast p filtered long reads and then we can see what tools this workflow is using the main tool is the fly assembly tool and all of the settings in there and then we're going to run some tools to look at the results of that assembly so faster statistics vantage image to get a um visualization of the actual assembly graph and the cost um genome report so we can just run this directly from here and that may take a little bit of time to run the workflow has now finished and all of our output files are at the top of our current history we'll have a look at the cost results so click on the cost tabular report and this gives us some really basic statistics about our assembly you can see the total length here is almost a million base pairs our largest contig is 235,000 base pairs and we have 152 contigs your results probably won't be exactly the same but they'll likely be similar we can also have a look at the cost html report and look at the actual contigs here just to get a visualization of the sizes and the distribution of sizes of contigs and we can have a look at the vantage image of the assembly graph and we can see that lots of our contigs have been joined here in this top left picture and we also have a lot of unjoined contigs some of them appear to be circular now up to the assembly polishing stage so I have imported the workflow and we can have a look at it in the workflow canvas this is actually quite a complicated workflow because it involves sub workflows so in here we have three main steps which are to polish the assembly with raycon then with madakar and then with raycon again but each of those raycon steps are their own workflow with their own set of steps including mapping with minimap and iterating that polishing steps several times so it's much simpler to view this when we paste in the sub workflow just as a sort of a single box here we can run that now and what we need to give it as input is the assembly that we want it to polish so this will be our fly assembly the consensus just double check that's the correct one this should be a faster file and that's correct so yes that's the assembly that we want to polish then we need to give it the long reads we want to give it the same set that we actually used in making this assembly which in this case was the output from fast p so we want the fast p filtered long reads we need to check this is the correct setting here we have oxford nanopore data so that's correct here and we'll also give it the on lumina reads and we can run that so now we can look at our polishing results we can look at first our original genome assembly and the length of that by looking at the faster statistics so the original length was nine million about eight hundred thousand base pairs and then after all these polishing steps we can see our final assembly is here it's the raycon polished assembly faster file and the stats on that are that the the assembly is slightly smaller which we would expect that often happens with polishing some of the mistakes get removed so now the assembly is about nine million six hundred thousand now we'll assess the quality of the genome that we assembled so we have a workflow for this that i've already imported let's run that we need to give it the polished assembly which is file 61 in this case your yours might have a different number but it's the raycon short reads polished assembly and then we're also going to give it a reference genome originally when we imported our files we imported a reference genome a rabidopsis in a faster file in this case that is not closely related to our data that we're using which is from a eucalyptus but we're just going to use that here as an example to see what the results might look like and we'll run that workflow and now we're looking at the results from assessing the quality of our assemblage genome our busco jobs are still running but we'll have a look at the class results so looking at the html report and we'll look at the content browser here and this will give you an example of how well your assembled contents have matched to your reference genome and ideally here you would put in a closely related reference genome we haven't in this case we've just used a rabidopsis but in a real analysis you could put in a closely related genome and then following that you'll also get results from busco which will show you whether the expected genes were found in those assembled contents so let's have a look at the summary of all things that we've done in this tutorial this is the flowchart again showing all the steps that we did with equality control using fastqc and nanoplot came accounting which used the tools merrill and genomescope then we trimmed and filtered the reads using fastp from there we assembled those trimmed and filtered reads using the fly assembly tool and we looked at some of the results in particular we used the bandage tool to look at the genome assembly graph then we polished the assembly using rakeon and modarka and finally we assessed the quality of our assembled genome using the tools busco and fastqc thanks for following today's tutorial on large genome assembly and polishing and we hope it's been useful to you whether using this test data or your own research data and if possible we would love any feedback that you can leave by clicking on this link at the bottom of the tutorial thank you


The fiery horse with the speed of light, the cloud of dust, and a hardy high old silver, the lone ranger. During the years of unrest that followed the Civil War, a powerful secret organization called the Legion of the Black Arrow sprang up in the western United States. Its members were to be found everywhere, defying the law or using the law for their own purposes, working toward the ultimate goal of revolt and the foundation of a despotic empire. It was the masked rider of the planes who led the fight against this band of outlaws and traitors, and for once his great strength and courage, his daring and resourcefulness were taxed to the utmost in the cause of democracy. Return with us now to those thrilling days when the west was young. From out of the past come the thundering hoofbeats of a great horse, Silver. The lone ranger rides again. Come on, Silver! Run the pill, the black arrow! Help, Silver!  Near Bitter Creek they saw a cloud of dust ahead of them. A word from Mask Man, and Silver increased his speed with scout keeping his place beside him. Now a herd of cattle could be seen moving toward the opening of a canyon. A solitary horseman rode the point, but just as the opening was reached, the leaders of the herd swung aside and headed for the rider, then suddenly they broke into a run. That's a girl in front of the herd! Come on, Silver! We've got to hurry! Get amongst, cow! Swiftly the lone ranger and tanto were cut in front of the herd, the girl ranged alongside. Desperation solid in her voice, she urged her mount on. Come on, Blackie! Run! They're gaining on us! Swing to the right! Keep away from me! Don't fight your horse! Let him swing to the right! It's your only chance! Blackie! Run! Come on, Silver! Keep away from me! To the right! The herd swinging toward the creek! The what? We'll make it now! Lean forward! Let your horse have his head! Run, Blackie! Come on, Silver! Get amongst, cow! You can rain up now! We're out of danger! Hold scout! Go ahead, Silver! I suppose I ought to speak. That isn't necessary. You take plenty big chance. Try to drive Herd into Canyon Loan. I wasn't doing that. It looks as if you were. What do you take me for? A tender foot? Why, if they ever got up into the hills, nobody could run them up for a month. I, uh... I was trying to turn them aside from the canyon. I see. What do you mean by that? Nothing. Don't you believe me, masked man? I... I was wondering if you'd noticed my mask. Well, I noticed it, but I know who you are. I heard what you called your horse. Don't you believe me? You're right, of course. It'd be almost impossible to round up the herd if they ever got into the hills. There ought to be a fence across the opening of that canyon. There is. There was, anyway. Somebody broke it down. Wrestlers? How should I know? You, um...  Guess that's all I got to say to you. Goodbye. Just a moment. Get up, Blackie! You must hurry. It not look like her tried to stop Herd. It didn't look that way to me either, Tunnel. I'd say it was just the opposite. Why are you doing that? I don't know. I think we'd better stay close to Bitter Creek until we find out. When we make camp, then you scout around town. Hi! Hi! Steady scout. Steady. What did you learn in town, Tunnel? Cattle belonged to a young rancher called Ted Donovan. Donovan, huh? The Circle D. Yes, I know that's the Circle D brand. Him sell ranch and cattle plenty soon. Go into freight and business. Freighting business? Ah. Me here talking cafe. Him talk with big man. Father, girl, we see. Well, Ted, did you mind stew meat up? Yup. And I got another good reason for quitting as a rancher. What's that? My main herd stampeded this afternoon. They didn't pull up till they got to Willow Grove. Well, you can drive them back, can't you? I reckon, but it won't be long before I'm driving them to market. What's the point of that? Why don't you just sell a cattle with your ranch? Just, I can't find a buyer. Now, I'll have to keep the buildings and the land. But once I get my money for the herd, I'll buy wagons and oxen, hire men, and start asking for freighting contracts. You'll get them, of course. Sure I will. Is there any dangerous trade from here to Cheyenne? We gotta have supplies around this part of the country. You know what happened to your brother? Angus. Why do you suppose I'm going into this thing? I'm hiring more than just drivers. There'll be a guard on every wagon. And I'm hoping that the gang that wiped out Johnny and his men try it with me. We'll be waiting for him. We'll be ready, too. Uh... Well, I guess I did my best. Nothing or nobody can change my mind, Angus. Which jelly could realize that? What's he been saying? Oh, the usual. You're local to try something like this. Getting yourself killed won't bring jelly back in. Somehow, I haven't got any choice.  You gotta pick up the reins where Johnny dropped. Matt, what tunnel here? The tunnel. How do you know that this man called Angus is the father of the girl we saw out on the range? Let me ask Parke about girl first. I see. This young rancher can't go into the fretting business if he doesn't sell his cattle. That's right. So the girl was trying to drive them up into the hills. That's right. That answers one question. This freight train being wiped out. When did that happen? One week ago. Well, we were up on the sweet water. How far away from Bitter Creek? Maybe a hundred miles to East. Any landmarks? Bar keeps say outlaw raid and midnight pass. That's where we're heading, Tunnel. Midnight pass. Three weeks later, Ted Donovan had reached Cheyenne with his herd. Beef was bringing a good price and in less than an hour he had closed the deal. When he returned to the hotel with more than enough in his money belt to start out in the fretting business, he found an old friend waiting the lobby. Janice, what are you doing here? Hello, Ted. Do you mean to say you've come all the way from Bitter Creek alone? No. Pause here. But I would have come alone if I'd had to. What for? What's the idea? Well, it isn't going to make much sense to you, Ted, but a girl told me to. A girl? I should say it doesn't make sense.  It won't make any difference to my plans. It can't. But I got to admit I'm curious. Who's this girl you're talking about? I don't know her name. I never saw her before. As a matter of fact, I didn't see her very good that night. It was awful dark down by the corral. This is getting worse instead of better. I suppose it is. I guess there isn't any sense in my going on. Anyway, I didn't come here to see you. There's another man somewhere around Cheyenne that'll listen to me, even if you won't. No, no, no, honey. That's no way to talk. You know I love you and I'll listen to you all day long. But I'm going after the crooks that killed Johnny. Ted, didn't you ever realize you've been Johnny's twin and looked so much like him that even I found it hard to tell you apart? Didn't you ever realize that those crooks might... What's the matter? There was a man over there with a scar on his face. He was looking at you. Where? He's gone now. He went up the stairs. Oh, Ted, I'm afraid. I'm afraid to say anything but fear what you'll do. I'm afraid if I don't, I won't find the masked man in time. Oh, I'm afraid. Who's there? It's Colin. Wait a minute. Mike, you gotta believe me. I gotta believe what? I haven't touched a drop today. And it's almost noon. That's a miracle. I want you to know that I'm telling the truth. Yeah, there'd be another miracle. Oh, listen, Mike, this is street. I just saw Johnny Donovan downstairs. You what? I did with my own eyes. I saw him. He was standing over by the desk, talking to a girl. She caught us high at me and he started to turn around, but I ducked up the stairs. It can't be. All right, don't take my word for it. Go down there and see for yourself. How could he be alive? Red got plugged nine times down in Tombstone, and he's alive. Yeah, yeah. Maybe he's talked already. No, no. They'd have come after us long before this. What would stop him? Carol, of course. He figured she was mixed up in it and laid off for her sake. Would he do that after? After what happened? Maybe he's out to get us himself. Yeah, if he turned around any faster, he would see. We gotta get him first, that's all. It's too early for Carol to be at the cafe. We'll find her at her house. Why do we want her? She made the mistake in the first place. It's up to her to fix things up. Come on. Are you going out? Don't worry, we'll slip out the back of the hotel. Johnny won't catch you just yet.  I don't care. I'm glad. All right, you're glad. Maybe you won't be so happy when I get through. You're not going after him again. No, Carol, I'm not going after him again. Oh, good. He proved he wouldn't go to the law. Besides, what if he did? What could he prove? We're not going after him again, Carol. But you are. No. No, Mike. You wouldn't ask me to do a thing like that. I couldn't.  He trusted me and I double-crossed him. All right, that's bad enough. You can't expect me to do anything more. I won't, Mike. I tell you, I won't. You don't know what the choice is, Carol. Do your follow orders and don't you? Quiet, Silver. It's Tonto. Yes, you're right. Someone's riding with him. Tonto Pine girl in town came this hobby. Bring her here. The girl? Masked men. I'm Janet McLean. Don't you remember me? Of course. I thought at first Tonto meant someone else. I know who you mean and I have a message from her. You have? She couldn't come to shy on herself and she asked me to come instead. She wanted you to know the truth about Johnny Donovan's death. The truth? Yes. I'll have to tell it just the way she told it to me because there's some parts I don't understand. She said you would. Please go on. Well, in the first place, Johnny was in love with a girl named Carol. Ah, her work in Palace Cafe. Through Carol, Johnny met some men who had a business transaction they wanted to talk over with him. This is the part I don't understand. Those men were members of the Black Arrow. That's all right, go on. Whatever the transaction was, Johnny wouldn't have anything to do with it. That's why he was killed. You were right, Tonto. They rode out of town ahead of him and waited at the pass. Ah. What are the names of the men? Could she tell you that? Just one of them. My graffiti. Tonto know him. And that's fine. They may be able to get proof against him through this Carol.  Tid's in town. He's in danger every minute. Have you told him all this? Is he going after Rafferty himself? No, I was afraid he'd do that so I haven't said a word to him. And I can't see why he should be in danger. They may think he's Johnny. Why should they? Ten and Johnny were twin brothers. They look exactly alike, didn't you know? Here's Silver. What are you going to do? Twins? He is in danger. You've got to find him. Is that it, Silver? Yep. Let's hope we're not too late. Get him up, scouts! Here's Silver! Hurry! The curtain falls on the first act of our Lone Ranger story. Before the next exciting scenes, please permit us to pause for just a few moments. Now to continue our story. As the Lone Ranger, Tonto and Janet were heading for the town, Angus McClain and Ted Donovan met on the porch of the hotel. This is the first time they're coming in, gentlemen. Hello, Angus. You're up to a late yourself. Where's Janet? Has she gone to a room already? Well, I don't know. You don't know? Why should I? I haven't seen her since supper. You mean to say she didn't go riding with you? I know. I had some business with a man named Smithers. We've been talking for the last three hours. But she told me... Well, did she? Did she what? She said she was going for a ride that I shouldn't worry. She had plenty of protection from a man I trusted. I thought she meant you. Well, if she had, she'd have said so, wouldn't she? She was smiling. I figured she was just being cute. It wasn't you she was talking about though. It was a mass man. The mass man? The Lone Ranger. What's that? That's why we're here. She's trying to find him. But if she went riding by herself outside the town of Misty, there's no telling what might have happened. Are you sure she hasn't come back? I've just been taking a turn up the street. She might have slipped in. Come on, we'll ask the clerk. He know. The Lone Ranger. Yeah, that's what he said. Who was the old gent? His name's McClain. It was his daughter that saw me. She's the girl they were talking about, eh? Yeah. Well, what about it? Has she come in? You were following orders. You've been sticking close enough to the porch to see everybody that come in. I have. How do you even see her? Ah, they're asking the clerk. Why should we worry about the girl? We gotta find some way to get down them and away from the hotel. This is it, Scar. Look, they're going upstairs. They're gonna try her room. Yeah? I guess the clerk wasn't sure. But as long as you are. Yeah, yeah, I know. And all we have to do is leave a message with the clerk before they come down. There he is, Carol. Come on, Scar. Getting back to the hotel. I'll fix the bar. I'll buy it. Thank you, ma'am. There you go. He's a good thing we left the horses out there here. Johnny, why did you have to come back? Open up. Hello? Hello. Is Janet McClain here? Won't you come in, Mr. Dunham? Where is she? Haven't you got anything to say to me? Well, I got your message. I came as fast as I could. Where's Janet? Is that all you can say? Of course that's all. What is this, a trick of some kind? Well, you might tell me a little bit about her. Are you going to get married? What business is that of yours? You don't have to feel that way about it. We could still be friends. Still be friends? Are you local? Not anymore. I guess I was for a while, but I'm getting over it. I'm getting over it awful fast. Why don't you sit down for a minute and have a drink? You answer me just one question. Is Janet McClain here or isn't she? Do you see her? There's another room. Belle? No, I knew this was a trick. I knew it the minute I saw your face. What would a girl like Janet have to do with someone like... That's enough. A gun. You're going to be sorry you said that, Mr. I can understand part of it.  I don't blame you for being sore. That part of it's all right. But nobody's going to talk to me like I'm dirt and get away with it. No, no, wait a minute. There's something wrong here. For the first time, Johnny, I'm going to be glad to pull this trigger. Why, you... You called me Johnny. I apologize, Mr. Donovan. But I'm not Johnny. I'm his brother, Ted. What? It's my turn to apologize. I didn't realize you were a friend of Johnny's. But do you mean to say... Hasn't anybody told you that Johnny's dead? It's true. I can see it now. But you're so much alike. We were twins. Now, steady there. I'm all right. Just to lose hope and then hope again and find out after all. But you were going to shoot me. My cold a turn. I was jealous. You were in love with Johnny then. Yeah. I see. Maybe you'll be glad to know this. I'm not going to rest until I find the men who killed him. It's better for you to go home and forget all about it. Can you do that? It isn't the same. You want those crooks to get off scot-free? I... No. No, I don't. I can't. That's all there is to it. They've got to pay. I'll find them and I'll... I'll help you, Ted. You what? I'll help you. And you're going to be mighty surprised at the help I can give. Thank you. Lodge. Did Donaldman get the message I left for him? For sure. I had to tell him how to get to Carol's house. But he left about 15 minutes ago. He had to tell him. Quiet. It's mighty funny. Johnny's old girl and Ted's girl getting together that way. Ted's girl, you say? Sure. He and Janet's going to get married, pronto. Hey, let me get this straight. Who is this Ted? Ted Donovan. Johnny's brother. You know the fella you left the message for. So Johnny had a brother. A dead ringer for him. If you knew Johnny, you couldn't miss the resemblance. Don't forget I... I didn't have a look at him. Well, just stick around. We can't do that tonight. Come on, Scott. Yeah. Brother. Why didn't you find out what his first name was? You saw him out here on the porch. You didn't bother to ask any questions. Well, did you? This way back to Carol's. Hey, what if she's built him already? I hope she has. But she won't. Talking to him face to face, he wouldn't take her long to find out the truth. And I don't trust her. Come on. He may be at the hotel. That's it up ahead, isn't it? Yes. We'll stop there first. You will, man. I can't be helped. I'll answer for him. Come on, Silver. Head above the couch. The whole truth, Ted. I swear it. I didn't have anything to do with the ambush. And as for the rest of it, well, you'd have to know a lot more about the black arrow to understand how you can get mixed up in it without really wanting to. You must feel bad enough, Carol. You're getting punished for your part in it. I am. So they wanted him to smuggle rifles to the Indians. And he wouldn't. I thought he could say yes or no, and that's all I'd be to it. Well, it doesn't interest me much what led up to it. Mike Rafferty killed my brother. Ted, you promised. You promised you'd go to the sheriff. He can't shoot any straighter than I can. No, you've got to listen to me. There's nobody in Wyoming got a faster draw than Mike. It's my job. Just tell me where I can find him. The scar is with him all the time. You'd end up the same way Johnny did. Where can I find him? Mike, I... I want you to meet Johnny's twin brother. This is Ted Donovan. Where's Ted Donovan? He's all right. This is the Lone Ranger. Is Ted here? Well, I know, Miss McLean. He went after you. After me? Where? Carol's place. Mike Rafferty brought a message you then wanted to see him alone. Mike Rafferty? Where is this place? Are you talking about a cafe? No, it's her home. I don't know where that is. Good, come on. Miss McLean, your pa's been looking for you. We can't stop. This is a matter of life and death. What's the matter, Carol? You seem to be kind and nervous. Why don't you sit down? I... I'm not nervous.  You're standing right in my way, Carol. I can't see Donovan's face. I want you to get things straight. It isn't Johnny. It's Ted. Hadn't you... Hadn't you better leave? Get out of the way. Go ahead, Carol. I thought so. You've been talking too much, haven't you? No. Oh, he's new.  You're the one who's talking too much. Well, maybe so. I won't waste any more time.  What happens then? You'd hang your show. You'd never get away with it. It's his life or mine and you know it. Look, Mike. Without me, he hasn't got any proof. Can we make a deal? Let him go and I promise... I swear I won't say anything. There's only one way I can be sure of that. All right then.  One murders as good as another as far as he's concerned.  Tie him up. Leave him here. Take me with you. You can't make any proposition like that with me around. Stand aside and I'll shoot it out with him. You wouldn't have a chance. Here goes, Carol. The lamp. Dish out the lamp. Get out there. Come on. Up with your hands, Mike. It's a lone range in the doorway. Let him have it. I got him. I saw him fall. No. No, he ain't there now. He ducked into the room. He's coming toward us. Keep your back to the wall. Shoot wherever he hears sound. You're making a lot of noise yourself. Over there. Do you hear anything? Nope. He must be done for. That's what I've been waiting for. What does the door mean? Mike! Don't shoot, Skaw. You might hit me. He's got my arms so I can't move him.  No, don't. Don't shoot. Let's hear your gun hit the floor. There. There. Where are you? I'm afraid he's unconscious. I pushed him down when the shooting started and I think he hit his head on the table. How about another lamp? I'm lighting one now. Here you are. All right, Tunnel. Tie these two up. Tunnel, do that. Ted, are you hurt? I don't think so. This might hurt. I'll help you stand up. If you turn us over to the sheriff, you've got to jail her, too. I'm ready to go. Did you help plan Johnny Donovan's murder? No. But I've been working for the Black Arrow. You can't put her in jail. It's her evidence. We'll send these two to the gallows. Are you willing to give it? More than willing. Will you help the government against the Black Arrow? All I can. She won't live to do it. Yes, she will. Get them moving, Tunnel. We're heading to the sheriff's office. Ah, you come with me. What did you tell them, Tunnel? I can't answer that. But it was all I knew. Certainly the masked man and Tunnel leave in a hurry. Just look at them ride. I hope they get there in time. Is it a long way? Yes. And the yards will be against them. That won't matter. Not even if they were a hundred to one. My bets are on the Lone Ranger. I'm sure you have just heard is a copyrighted feature of the Lone Ranger Incorporated.


Hey guys, it's Nate, aka The Foot Account, and welcome back to the channel. Sundays usually mean icon upgrade SBCs on FIFA. And today there's one SBC that I hope EA released to bring excitement back to the menus. Today also we'll focus on how to prepare for that SBC and whatever icon SBC drops in case it's not that one. And of course, take a look at the new ShapeShifter cards that are going to be coming in packs today. Three more ShapeShifters as a part of the mini release are expected. And we have to talk about this market, guys, because prices are dropping like crazy. And I want to talk about why there is massive panic selling on this market. So all that and more in today's video, if you're excited for it, drop a thumbs up on it and subscribe if you're new. First, before we dive into a quick content overview from yesterday, take a look at EA's Twitter. Guys, it is July. Welcome to the new month. And also welcome to the month where we will finally learn news about EA FC. They've already changed the Twitter handle. They've changed the bio. The website is still the same though. Learn more this July. So we haven't learned more yet, but more information is coming and you can see EA has already started that rebranding. So that July 1st date yesterday was definitely a turning point. And I'm hoping for more information coming soon on content for EA FC. So that's just something I wanted to point out. But let's talk about yesterday's content really quickly before we get into the gist of today's video. First of all, couple interesting SBCs yesterday. First of all, these hero player picks, man, I don't know. I just think they're behind the curve. The weight on these is so dead. I know it's a one of five. But, you know, yes, you could get like Lucio and you could get somebody insane. Like you still could get a fantasy foot Genola or an aloe vera. And I get it. But still, the weight on these is so bad, at least the price is cheap. And I just think a lot of people at exactly 91% of people according to foot bin are not a fan of this SBC. I consider myself part of the 91%. And I'd rather do an icon upgrade pack or something today than this. But that just might take there. It is a cheap, fun SBC to craft if you've got fodder and you could get lucky. So I guess that's my thoughts on that one right there. It didn't really make fodder move that much. It's not that expensive, 85 and 86. No extra requirements in there. So there's that. And then also yesterday, we had an icon SBC that was the biggest W of content, Zola. Shape shifters Zola, which it's just a small shape shift, right? From striker center forward down to center, attacking mid. You can also play left mid, left wing. Five star skills, four star weak foot, five foot six in height. So he's got the very good dribbling stats, very low, kind of like a Ben Yetter-esque type of player. But I think the price here is what I'm super happy about. Really, really good price, like 300,000 coins. You can get the loan for a gold squad if you just want to try them out and friendly or whatever it is. If this is a card that you just want to do to have some fun, I like it. I like the value here because if you compare it to like Del Piero Trophy Titans or even if you kind of compare it to like the Luis Figo card that we have in this current promo team, he's 300,000 coins. Luis Figo is like, what, five, 600,000 coins to that striker card. They're not a one-to-one comparison, but W from EA on the dynamic, the stats, the skill upgrade, everything for Zola. I am a fan of that one right there. Now also yesterday, we had some store packs, a big 500K pack, which a lot of people were talking about as a really, really crazy pack. Of course, it's a 500K pack, guys. It's going to be crazy. 100 gold players, 87 and overall, 10 are above that E7 rating, and then one is going to be 89 plus 61% of a premium shape shifter's player. I mean, most people are going to be packing Pepe or Goetze or Nesri from this for sure. 22% for a shape shifter and 36 for a shape shifter icon. It's crazy how there's a higher percentage chance that you pack an icon.  Soul Campbell. But a higher percentage chance for that than absolutely packing a shape shifter's player, which is kind of crazy. So that was there. I also saw there was like a 10K pack. And honestly, guys, when I saw this 10K pack, I think I literally laughed out loud. Three gold rare players for 10,000 coins and they're intradable. Why? There is no point into this pack, man. I know it's 10K and if people are opening it, it's because it's 10K, but like, why? Three gold rare players. Like, no, I don't know. I just saw that and I kind of laughed because that was like, it's not worth the 10K, man. Even not even worth the 50 fever points, but I digress. Let's talk about Sunday content today on this game and hopefully a really good icon SPC. Let's start there, right? And I want to go back and look at the icon SPC that I'm hoping to see today. Will we see the return of the 90 plus Brazil, France, Dutch icon upgrade pack? Because this was the one that was released a couple of weeks ago during the end of the season and it was a banger. It was 300,000 coins to complete. I think we can only do it once when it was released and then it didn't come back and I was really hoping that this SPC would come back last week, but the 90 plus player pick came out once again. Man, I love this pack because not only did I pack Ronaldinho from it, but I just think this gives you the best chance of packing a card like a Ronaldinho, like a team of the year holit, like a trophy Titan Zaddan. This is your best opportunity to get one of those cards because the player pool is so small compared to normal icon upgrade packs. Now the thing is this was only 300,000 coins last time and this 88 rated squad was 171K. I think right now Zola has an 88 rated squad inside of it. Right? How much is that costing? 120,000 coins. So if they drop this icon SPC with the exact same requirements, 85, 86, 88, it would probably end up being a little bit cheaper. I do believe 85s are maybe a bit more expensive than they were when this was released, meaning 50K for an 85 rated squad. It's pretty cheap, but 170K right now or what it was then now being 120K for an 88 rated squad. I love that. I would imagine they would keep the requirements the same or be very similar to where they were before if this was to be re-released, which is why if you're thinking about can I invest for this SPC or whatever icon upgrade we do get today? Absolutely, right? It's all about on Sundays, the icon upgrade packs. If it's a good one like that nation specific one, we're going to see some fodder rise. If it's the 90 plus player pick again like we've had for the past couple of weeks on this game, then it's probably not going to rise that much, right? That was the story last week with fodder. We invested in 87s and they didn't move until Monday because Monday was when we got the 84 times 10. Now we're hoping for a rise today. I like the 88s the most. I really do because I think they have the biggest kind of like rise potential with an SPC like an icon pack coming today. 88s are about 15, 16K. I love that price. 16,000 coins is good. I'm going to give you guys a tip on investing in fodder right now that I think is so key by gold cards, especially if you're like Nate, I think they might, you know, release other SPCs or you know, you think that 91s might be a place to invest. Invest in the gold card specifically on these higher ratings. I mean, speaking of 91s, De Bruyne is 25K and Van Dyke as a 90 is also 25K. That's kind of crazy that 91s are cheaper than 90 rated cards. Doesn't make a whole lot of sense, but the Tots prices are messing this all up. Anyways, if you're going to invest in fodder, invest in the gold cards because number one, that's just what people think. When they think about putting cards into an SPC, you think about putting in the golds, right? You think about putting in these players that's, you know, you're like, yeah, they're fodder. Yes, they're some of the cheapest cards in the game. I mean, unless you're looking at solutions, who puts a shadow on Holland, by the way? That's just crazy. A lot of things going crazy in this video today. We're all over the place, but when you think about putting players into an SPC, most people that play FIFA, think about putting in gold cards. So that's why I would say if you're going to do a little bit of investment or a club stock as I'm doing right now, buy the gold cards. You can still buy some of the other ones too because informs and out of pack special cards do have good rises because they're still in demand as 88 rated cards or whatever rating it is. But the gold cards specifically on like a Kevin De Bruyne, these higher rated cards as we have a lot of team of the season cards that are in packs right now, getting supplied. I mean, these gold ones are the way to go because you will see them rise higher since they're not getting supplied. And this is what people think of when they think of fodder cards. So that's one tip that I would give you right now. I like the middle to high tier fodder, 87s, 88s, 89s for specifically an icon pack coming today. You could look into 85s. 85s at 7K, it's not terrible. Ooh, 7.3K. Okay, they're going up a little bit. I know these guys were about 6K last week, but we've seen 85s be eight, nine, 10,000 coins. They could go again to about nine to 10K this week. It just depends on the rest of the content that we get. So I think I like 85s and 88s the best. But of course, do your due diligence. Do a bit of a club stock. And right now is a good time to do a club stock anyway with a lot of SBCs out and fodder usually does rise a little bit during the week. So that's kind of my fodder investment tips for this weekend for hopefully today on Sunday, getting a very good and a very exciting Brazil, France or Dutch icon pack. If they drop that icon pack, I'm going to be doing it like right away because man, I'm telling you guys that pack gave me a Ronaldinho. And again, that's a pack that I just want to do because of the player pool and the potential that you have to get something sick. So that's what I would do there. Club stocks and fodder. Now, what else are we getting today? We're getting a mini release. That's right. We know Sunday mini release days and it's all about this card today. Kyle Walker. We didn't see him on Friday. We wanted to see him and he's finally dropping today. According to foot sheriff, he's got to be a goalkeeper card in my opinion. That has to happen. A center-back Kyle Walker would be cool, but a goalkeeper Kyle Walker, which is hit different for the memes and everything. It would be fantastic. I'm so intrigued to see what EA do with this car today. Do they find a way to make him like a meta goalkeeper because six foot in net is not going to cut it, right? We've talked about that. So curious to see what the Kyle Walker looks like today. Balak as a icon shapeshifter is lead for today as well. And then Dembele from Leon. Interested to see where they put his card and the stats that he get him. They give him, but he's probably going to be pretty cheap and basically fodder. So watch out for that today. Other content that we could see, maybe a crafting objective. The crafting SBC still has, I think it's got six days left on it. So they might drop something in objectives today. They also could do a player SBC. I'm not entirely sure. Usually with mini releases, it just kind of depends. Like it could be a day where it's just the mini release in an icon pack and that's it. Or they could throw a couple other things in there. We will just have to see. Of course, we have the regular refreshes, right of the tots, the shapeshifters, the daily login. Make sure you get that done and the tots moments or tots upgrade. Wow, two player pack here. We get a walk out. We are getting a ter... No, you're even better than terstagan. 90 rated. I just wanted to open these up really quick because these are my daily packs from the daily login and 83 times three was yesterday's. And then today on Sunday, I think we get a... No, sorry, 83 double. I think on Sunday today, we get an 83 three or an 83 for one of those two. So again, keep building up, keep doing those sick. I get two 83s. Absolutely love that. Now let's talk about the market because I don't know if you guys have noticed or not, but prices on a lot of this game are absolutely in the mud and it's not necessarily with the cards that are and impacts right now. It's some of these cards that are in the team of the season squads and the cards that we talked about, especially on like yesterday's video and even a little bit earlier than that, when we saw the tots upgrade pack dropped, that tradable tots pack, we knew it was going to impact the market a lot and it is. Look at Gabriel Jesus, 715,000 coins for this card right now. Guys, I remember looking at this card on Thursday, Friday at 1.1 million coins. Look what he has done in the past two days. Yesterday specifically on Saturday was a big time sell-off day. He went from a million coins now to where he is at 700,000 coins. Musa Diabi is another one that dropped off a bunch. He started at almost, I mean, he was 400K on Friday, 433K. He dropped from 360 on Saturday all the way down to where he is now 280,000 coins. I actually just picked up a Musiala at 700 flat. He's going back up a little bit because these guys, they're rare, right? They're getting supplied but they're also pretty rare at the same time so you see the fluctuations. I mean Diabi was just 270, now he's 297. So watch these cards for the fluctuations but the real kind of message right now and what we're seeing is these prices are absolutely in the mud and I want to talk about it. Why is this market dropping as much as it is? Well, the first thing is we saw the leaks about footies, right? Footies, the upcoming promo and we know what happens during footies, right? We know what happens. It means cards are getting re-released back into packs and that means supply and that means prices drop. This happens every single summer during the footies promo. You see a team of the season cards, other out-of-pack specials, team of the years even, drop a load in price because there is so much new supply and then people pay an excel ahead of that before the cards get re-released because they know that they're going to be dropping in price. So it's a combination of that selling right now of people fearing the footies is coming next and these cards are going to be put back into packs. That's reason number one. Reason number two is these Tots cards like DiBala.  The daily SBC to still pack a Tots upgrade, to still pack a guaranteed Tots or Tots moments card every single day is still dropping. He's got look at DiBala. He went from 618,000 coins down to 470. He's back up to 520.  But I'm telling you guys if you have not sold yet on some of these players that you maybe have still in your team. Guys, these prices are still going to go lower 100%. I mean, you talk about a guy like this Osemin who was 1.9 million coins on Thursday, 1.5 mil today. You guys are going to think I'm crazy but this Osemin card is literally going to be like five to 600,000 coins in this next month, right? I know that's a month. It's a long time away from now. But whenever he gets re-released back into packs that's the type of market crash you're going to see on this game during the summer. You're like, Nate, no way. Yeah, wait, it's going to happen, okay? So Osemin 500K, see you soon. Stuff like that is where you're going to see the craziest market drop-offs. Now these Tots cards, I'm going to put this out there as well. Footies batch one and we're going to talk a lot more about Footies later this week. So I don't want to dive too much into it right now, especially because we don't 100% know that it is the next promo. We have the leaks and we have the speculation but it's not confirmed yet. So that's something to say there. Not all of these Tots cards will get released week one. There's going to be a lot of panic beforehand. Some cards that are not in batch one that are not re-released in packs will end up going back up this weekend or whenever Footies starts because they're actually not in that first batch. So that's some kind of like theory stuff and some high tier kind of, I guess just market stuff that we're going to have to figure out later on this week. But for right now, there's a lot of panic. I saw Frankie's young yesterday at 1.5 million coins. He's back up now to about 1.6 but I sold mine that I bought on Friday. It took a little bit of a loss actually because I know that he is going to be falling down further in price as we head throughout this weekend. I expect more prices to drop today specifically on the team in the season cards because they're going to get supplied once more. If we get good content today, think about it, if we get like a 90 plus Brazil that nation specific icon pack or just a good piece of content SBC for an icon regardless, that's going to drop some more prices. And again, I think it's really the continual panic selling on a lot of those cards because they're fearing that they're going to get re-released back into packs. Now, at the same time as those prices are dropping, you do have some prices on these cards that are in packs dropping as well. And they're still fluctuating guys. Really good opportunities to trade yesterday. Hugo Yeriz went from like, I sold them too early, right? I know I was not really going to look at the market yesterday, which I didn't. But I sold Hugo like at bottom right here, sold them about right here. And he went even higher. He went to a million coins. So really good flips yesterday with him. I think Schweinsteiger went up. I think Pogba had a couple of rarity spikes. Klosterman's really rare. Watch his card on the market. He's really good to trade with. And again, there's still demand for these cards. Shapeshifter's team two and team one as well because in the, whatever it is, the cup mode or no, it's the Shapeshifters. Yeah, it's the Shapeshifter's cup where you have to play with three Shapeshifter's cards in your team. That is, that's a demand, right? There's demand there for people to play with these Shapeshifter's cards especially on the cheaper and lower tier. And that's why you see some of those cards like Zaha, Adiyemi, Raquelme from last week's team doing very, very well right now on the market and staying in inflated prices because people want to try these cards out and they're using them in the cup. So that part of the market's doing pretty well but the rest of the market guys is really not doing so hot. We'll talk more about footies and cover a lot of that during the week this week later on once we get some more confirmed news. But that's kind of the state of the market right now. EA, drop a banger icon pack today. I literally just bought some fodder. I'm ready to do it. I got to go play some more weekend league games and get this account rolling and get ready for an icon pack today. I'll be streaming today. That's the plan. Check the link down below in the description if you want to come by and check out the stream. Whatever content we get, we'll be reacting to it. Maybe making some buys, opening some packs, just having a blast, summertime on FIFA. Good vibes man. So link is down below in the description. If you enjoyed the video today, drop a thumbs up on it. Comment down below if you do have any questions and of course, subscribe if you're new. I'll see you guys in the stream today. It's been Nathan Foot account. Catch you guys there. Peace out.


Okay Twas the day before Christmas and all through the shop Tom was making more videos. He just would not stop It's a holiday. I cried. Can't you take a break? What do holidays matter Tommy asked when I have more videos to make But there's still presents to wrap and food to buy. What can I do to get through to this guy? Something needs to be done. I thought with a sigh then I squared up my shoulders. I'm gonna give this one more try I stomped into his office and demanded he stop give it a rest Tom. You're over the top He looked up from his camera his eyes opened wide. Why are you angry? He asked his head cocked to the side My viewers want more. I can't let them down if I keep going. I'll be the most famous in town At that moment I knew then nothing I said was going to get through Tom's very very thick head It's not worth a battle. I thought as I pulled up a chair Sitting down at his side. I took a deep breath of air How can I help? What can I do? Tom looked at me and smiled. You can make videos, too That's why she's here for us to see you Merry Christmas. Happy new year all the holidays happy holidays to whichever one you're celebrating or not celebrating It's your choice This we want to say thank you for watching you made it to this video Just appreciate all of you and I'm here in Christmas Eve because we're actually gonna produce more videos So I just got to get them scheduled and published I recorded this on Christmas Eve because apparently on Christmas Day I'm not allowed to no he's not


It takes time to build an authentic business, one that is deeply meaningful to you and can support you financially. When I look at some of my heroes, Seth Godin, who is one of the most respected marketers in the world, it took him, as I look back in his blog archives, two years of consistent blogging almost every single day until he started getting people in a large amount of people sharing his blog posts and then he became world famous. Two years of consistent, almost daily blogging. Are you willing to do that? If it was worthwhile, if it was truly meaningful to you, then I hope you will say the answer is yes. Another example, Gary Vaynerchuk, who built up a business selling wine. It took him 18 months of almost daily videos creation before he really saw the online wine sales take off. Wine library was his YouTube channel. It took me, I noticed, about two and a half years of consistent making videos and writing blog posts before I realized one day that I have a full practice and I haven't needed to reach out for people to hire or refer me because people were just coming to me based on my content, based on word of mouth, which is largely from content. So why does it take time? Why can't you just create it in 30 days or 90 days or whatever is being promised to you that you can easily build an audience in X number of months or whatever? Because it takes time for you to come to an understanding of what you really want to say and commit to saying in the world. It takes time for you to come to an understanding of whom you really want to talk to and serve in your business. And it takes time for people to trust you. People don't really trust you just by seeing one thing from you or by seeing your content for a month or two or three. It takes months of being consistent until they realize, oh, this person is going to be around. This person is trustworthy. They are continually helpful. They're continually uplifting or whatever it is that you share that's authentic to you. It takes time for you to finally understand what it is that people need and want from you so that you can create product and services that are so interesting to them that they really are going to buy it. So what do you do with that time? It might not take you two and a half years like it did me. I hope that if you follow my content, watch my videos or read my blog posts, maybe it'll take you only one year. Maybe it'll only take you six months. I would say that's probably a minimum, especially if you use paid advertising, it'll shorten your time. But it's going to be extremely rare if someone says they could do it in 30 days or 60 days or something like that. So what do you do in the six to 24 months that it's going to take to build an authentic business? Let me give you sort of an overview of that timeline. I'm going to simplify it for the sake of the video. The first thing you're going to be doing is to do authentic content marketing to build an audience who really just appreciate and like you for who you authentically are. As you share content that is meaningful for you, whether you created it or you share what other people have created that's meaningful to you, people start to follow you because they like your curation or they like your voice, whether it's speaking voice or written voice. And they like your personality. Authentic content marketing means that you don't try to show up with a kind of polish that isn't you. Just show up as yourself. Do the just just be yourself because then you'll draw forth the people that you don't have to pretend with. So start with authentic content marketing. And then after you do that for about a third of the time, so let's say you're thinking of like a one year timeline. And after you do that for about four months of consistent authentic content marketing, just focusing on being yourself and offering meaningful content to audiences and hopefully buying some paid ads for people to see your content. Then you layer on top of that a focus of engaging with your audience, having conversations individually, you reach out individually to audience members that have become subscribers and are continually engaging and maybe sharing your content for the people who are doing that. You engage with them in one-to-one conversation or on social media or through surveys and to find out what is it that they need in their life that you could see yourself providing a product or service for. Now you don't always have to be the one creating the product service. You may do that, but you might also find somebody, some other business that has a product service that you can partner to bring that in front of your audience and therefore earn a livelihood doing that. That's called affiliate marketing or joint ventures. So that is going to be after four months out of a year, you layer on the focus of conversations for another third of the time, for another four months essentially. And then after that, the remaining third of your timeline that it's going to take you to build an authentic business or at least to get it to a level that you can see it becoming sustainable. The final third is offer building, meaning based on the conversations you've been having, you can now start to create products or services or to curate them, put them online and to check it out with the people that you've been conversing with. Hey, is this going to meet your needs? Is this something exciting for you? Would you buy it and actually make the offer? Make the launch and announce that product service. So this video is getting a little bit long and I'll include more information in the notes of the video, but I hope that helps a third of the time focused on authentic content marketing and then the next third layering on top of that conversations or I call that niche interviews. And then the next third layering on top of that offer building and launches and tweaking your product service until you get it to something that people are so excited and would love to buy. So if you follow this kind of timeline, you'll be able to, you'll, you'll give yourself a much better chance of building an authentic business that will be able to sustain you and not to have to give in to the quick, easy money scams and hypes that are out there very frequently. So remember that anything of value, anything of true and deep value takes time to build and the time to start is now. So I am open to your comments and questions as always and I wish you joy and fulfillment along the journey of building your authentic business.