Hi and welcome to the NCLA government resources section help I'm an accidental government information librarian webinar series. Rich gauze is the government information librarian for the university libraries at the University of Central Florida and has coordinated establishing the centers of excellence at UCF for information from the US Department of Energy, Atomic Energy Commission, Nuclear Regulatory Commission and the National Aeronautics and Space Administration. Thank you very much Rich for joining us today. So my name is Rich gauze I am at University of Central Florida and I'm going to go through kind of a whirlwind in terms of resources mostly an awareness of what's out there and the link that you're seeing on the screen for the Florida government information research tips is the only link you need to record in terms of getting back to the resources. I am in the process of adding a listing of every resource that's in the webinar to that page. So as I flip past the screen you don't have to worry about trying to capture the URLs that are shown and the titles of certain works because that'll be there. So where do we start searching? Well a lot of people are going to search their their favorites in terms of and if you know what you're looking for I think that's one of the things a lot of times just knowing what to look for then you can just go to Google or Bing or Wikipedia or some other source. These are three of the sources I would go to probably next and I'm going to go into more detail on each of them. MyFlorida is the MyFlorida.com is the official state of Florida website. The state library of Florida online catalog has a tremendous amount of resources and then the shared academic catalog for the state universities and state colleges of Florida to be the next place I would look and I'll touch on a couple of key dates that have an impact when you're looking for Florida government information throughout the presentation and we'll hit each of these as we go through. Before we're a state we were territory so you're not going to find state government information if we were in a state but you may be looking for Florida information from the earlier period and really want to start out in terms of looking at the levels of government as to what type of information and the information sources that you're going to look at. I will often start if I'm looking for information about the state of Florida I'm very likely going to be in federal databases and publications instead of looking at its state of Florida. The federal resources may be easier to find and maybe a little bit more stable and if I'm looking for information at the level of Florida rather than counties and cities and that type of information but I probably wanted to compare that data with other states and so the federal resources likely to give me that data from a common source to review. Also there are a number of publications that may have been produced jointly by a federal and state agency for example the U.S. Department of Transportation and the Florida DOT or the USGS and the Florida Geological Survey. Also I'll look at federal publications because they may cite state publications and now that I know which state publication I'm looking for I can search on Google or other sources or in state resources for that specific publication. I'd also go to federal resources I'm looking for Florida when it was a territory because I'll be looking for example the territory papers of U.S. which many of you may have volumes 22 through 26 come in the Florida territory and the similar wars which overlap the 1845 Florida becoming a state and maybe looking at federal material there both in terms of the war department or in terms of Indian affairs. If I'm looking below the state level at counties and sub areas be aware that there's 67 counties in Florida and you get that to I'd also be looking for smaller areas and probably looking at the federal resources for example the census. You also have the counties grouped into 20 metropolitan statistical areas for the urban areas. You can do sub counties there's 316 county subdivisions for example my home county of Orange County has six subdivisions in the federal resources for the census and you can look at places but place boundaries tend to change over time a city may grow or shrink it may cease to exist there are 411 incorporated places in the state of Florida plus another 509 other places that are clearly identified but they're not incorporated so if we go back in time in terms of looking at material in Florida go back all the way to the Spanish period you had east Florida and west Florida you had st. Augustine or the east side and you had Pensacola out almost into Alabama and that's really where you start growing from there we started out with the Scambia County and St. John's County were the two first counties in Florida representing the west part of the state and the east part of the state as we approach here's 1830 in terms of looking at the county boundaries this is overlaying in the dark lines what the the county boundaries were at that time and really the state of Florida was the the pan have between there between Pensacola and and St. Augustine because you didn't have mosquito control air conditioning the lower part of the state wasn't really developed in fact you'll notice that Mosquito County was the name for the county that I currently reside in which is now Orange County by 1925 the county boundaries had stabilized and so I generally use county boundaries when I'm looking for most information at the sub-state level 1920 is shown here at the very last of the changes in county boundaries took place in 1925 there have been minor changes since then if a river is the boundary between two counties you may find that it shifted a little bit but I'm not going to really worry about that. Jade County is now Miami Jade County that one point like 20 years ago there was a bill proposed to split Monroe County into the keys and it's two counties the keys and then the mainland part of the county but nothing ever happened with that. There have been proposals over time one of the things my my I grew up in Brainton over in Manatee County and there was a proposal from Sarasah Lentage Flagers to change the county boundary by about 150 feet I believe because a subdivision had a new section that was overlapping into Manatee County and it almost went through but the state legislators from Manatee County suddenly realized that really what was up was that the county boundaries shifting 150 feet to the north would mean that the Sarasah to Brainton Airport which is right on the county line all the runways and all the noise from air travel would still be in Manatee County but the terminal building would now be entirely in Sarasah County and all the tax revenue would go to Sarasah County from all the flights at the airport so that didn't go anywhere either. There's also sub areas within the state in terms of counties a couple of different regional areas within the state that are state functions but they're not under a state agency directly in some cases and then there are sub areas for state agencies and then the court system and one of the problems you'll encounter is that these make these are groupings of counties to make up these districts, councils, regions but none of the agencies as far as I can tell uses the same division of counties so if you're looking for two or three counties that are near each other you may have to go to different regions to pull the data for them. For example the farter regional planning council divides up the state very clearly and if you want to pick I'm going to go over a series of maps if you want to take a look at the area in peak the Tampa Bay area, Polk Hillsborough Manatee County and just sort of try and track how that changes as we move from area to area to see that the local health councils actually split that up into three different overlapping into three different areas. In environmental protection the water management districts actually there's five water management districts in Florida and these are the ones that don't conform to county boundaries they're based on the watersheds so you'll see the one that's highlighted in blue is the area that flows down through the Kissimmee River down into the Everglades. The northwest Florida water management district up in the Panhandle is the area flowing out of Alabama and Georgia the rivers that flow down here actually the Appalachicola River that flows down into the Gulf actually starts as the Calusa Hatchey River up very near the North Carolina border and flows all the way down around Atlanta and down to Florida. The State Department of Environmental Protection, the water management district, the State Department of Environmental Protection are linked fairly closely but they have different district arrangements of those county boundaries how they get grouped. State Department of Transportation is decentralized and they have transportation districts and again they're grouped differently. State Division of Emergency Management deals with hurricanes and fires and all those sorts of things that we might worry about again groups them differently so here you can see all those maps sort of side by side and you'll see that comparing them one map to another that none of the counties are grouped quite the same with any one map so this is just heads up if you're looking for Florida information that you may have to look in different groupings to find that county level data. Court system in Florida the same thing the five district courts that are the appellate courts in Florida each of them is subdivided into circuit courts which are also subdivided into county courts but this is the difference from the federal system to the state of Florida system in the federal system the district courts are the trial courts the smaller courts and the circuit courts are the appellate courts in Florida the circuit courts are the trial courts and the county courts and then the district courts are the appellate courts so getting back to one of the places I would say start the myflorida.com website gives you the really a good starting point for most people to try to locate information about Florida. I broke out the tabs the tabs are the top left visitor Florida veridian business government here's sort of a breakout of how those list and group information for folks and so depending on what type of information you're looking for they give you a good start to trying to find information in the top corner of that myflorida page there's a fighting agency tab and that opens to this page which is if you know that you're looking for particular type of information this actually is a directory of the Florida agencies you'll see the environmental protection in the top middle of that column and I'm going to come back to that just a moment this is the lower part of that page so if you were looking for example the water management districts or the regional planning councils this provides you with those links I says we'll go back to the department of environmental protection so here we are on the page I've dropped down the division listing and we're going to go to the Florida geological survey in just a moment but I want to scroll down to the very bottom of their main page where they have a document resource hub where you can actually search for documents so if you know which agency you're looking for publications from you may find they've got a specialized tool they actually have a couple of specialized tools at the DEP for locating documents and publications from that agency if we go to the Florida geological survey page they have a link for FGS publications you go to that you've got a downloadable list of the FGS publications as a PDF which they update about once a year there's also an online list but I'd recommend the downloadable list because there you're going to find about 80 pages of information listing all the publications even those that have not moved online yet although many almost all of them have at this point and then the individual pages there they give you information in terms of ordering you sometimes you'll find pricing but they have the links to the PDFs of the actual publications right there in the document on the right page you'll see the open file that series there's a bunch of them that don't have links that's because there's a general link at the top of that column that will provide you all of those geological maps in one place going back to the my Florida page there's a tab at the top for the forward one directory which is the directory for the state of Florida including the state information center if you're trying to find you can't figure out who to contact but you'll notice the left hand side you can look by agency by county you can actually look by employee if they're listed by the schools and the university settings the other thing I'll point out there is the email dating standards link I've done the drop down for citrus county or for the department of citrus rather and if you found in some other format in a bibliography or from presentation somewhere you found the name of an individual but you don't have their you actually have their name but you don't have their email address this will show you how for that specific agency email addresses are constructed so you can give that a try if you can't find them listed someplace the second place I was identified was the state library of Florida and for state publications this is going to be the main source if you you're not finding it elsewhere this is a great starting place the state library of Florida online catalog if we click I'm gonna come back to this page several times when we click on state publications in that where I've got the red air reporting we land on this page and this is we'll come back to this page again in a moment but this will let you search the catalog and it is presetting the search requirements to limiting to the state publications collection I did the search there for division of administrative hearings and your report and pulled up a listing for it including the where they've got digital copies the electronic resource list and this word they've got the links to the individual digitized publications now this Florida public documents collection that state library maintains you sort of have to go through the library catalog to get to the individual publications going back to the state library of Florida catalog page we'll click on the Florida information finder where the arrow is pointing and this gives you a lot of ways to find information about Florida by different topic areas I'm going to look at the A to Z index here and this will actually you can just work your way through a menu that will help you locate information from this A to Z list it'd be very helpful try to just sort of browse through find oh that's what I was looking for going back to the state library catalog page the state library provides a lot of services specifically for state employees and if you're looking to state catalog what you have to do is you have to know what you're looking for pretty much you can do a search of my keyword there's double ways to search it but if you don't know that something exists you don't know how to search it they put together these state agency portals that include a lot of subscription services so they can be good for identifying information but you may not find that you have access because you're not an employee in the state of Florida but navigating through their pages one things they've got are the state agency information lib guides basically the resource guides that they put together on their site and scroll down into the agriculture guide noting that they're listing various publications and they've got links to the electronic versions of those I'm noting that the Florida Department of Agriculture and Consumer Services and report they've actually got it digitally available back to 1890 again in the Florida Public Documents Collection so you got these links to get to the old reports but you wouldn't necessarily have known to look for them if you didn't know that they existed or what the titles were back to the state library catalog page and we're going to go to the classic catalog which is the older version to look at one resource that I've very useful the Florida Biography and Citation Index is essentially the references from their vertical file at the state library about Florida I do a search here for Henry Flagler and I find an entry for him I select that entry and that's great we've got his birth and death date but there's not a whole lot of information here you need to look inside this record and you don't look at the a look inside you look click on the catalog record to look inside this and what they've got is citations to various books and articles to go look for it and you'll really have to go look and see if you can you find access to these or interlibrary load them but this does give you a lot of information that's really useful in terms of looking for information about Florida and particularly about people in Florida people looking for information about Florida don't really care whether it's published publications information that the state library maintains or state records that are unpublished Florida documents that are maintained by the state archives but they are separate catalogs for looking for that information the state archives catalog is fairly powerful but I find it to be fairly finicky when you're doing searches by keyword to try and locate material I've got the results here from searching for John's committee and I needed to put that in quotes because otherwise it looked for John's or committee I got several hundred results by putting it as a phrase by putting in quotes I actually limited to just those entries that had that the trick here is you need to click a little number on the left hand side it does say click on a number below for a detailed description most people don't see that and don't realize that that number is actually providing you with the detailed information about that the John's committee was Florida's version of the McCarthy un-American activities committee that took place at the federal level they were looking for at communists subversives in about the second half of it it really focused exclusively on trying to route out homosexuals in Florida in government agencies and in the university system on this page one of things you'll see is that little folder is also clickable and that actually will list you several pages of the individual folders that exist in the state archives of transcripts of the interviews all the different types of information that was collected going back to the state archives catalog the advanced search screen is really useful in terms of helping you understand how to do an effective search they give you examples for how to fill in the information on these search forms these tips at the bottom in terms of searching I also found that the browse indexes so you're searching a catalog and you don't know that stuff exists you don't know what terms to put in and so the computer gives you back what you told it to look for the browse indexes feature actually allows you to browse by subject terms or by person's names right then we go back to the previous screen you notice down at the bottom subject access fields that actually lists terms that you could use in the catalog for doing searches so I pull up this entry browsing beginning with Flagler to look for Henry Flagler again and actually find six entries that are resources in the collection the it's something you wouldn't necessarily know to look at without digging into them the farter keys overseas railway in terms of great rare roads to fire that makes sense and the Key West railroad bridge construction photographs that might make sense but you have to drill into these files to really discover what's in them back to that state publications depository program page we're going to take a look at the collection policy the quarterly publication reports and the list of foreign depository libraries so this identifies what the state depository program which has been going since 1967 for distributing publications around the state of Florida that are received by the state library if they don't get enough copies to distribute they only have them at the state library but otherwise they will distribute it to the various libraries currently there's about 20 state depository libraries scattered around the state so this tells you what they do collect and then what they do not collect things that will not be available in their material you might find them in the state archives collection this is the public documents distribution list basically they've got it digitized back to 1998 and so this will actually list what was distributed to the libraries in that year the recent ones give you actually quarterly updates or the annual accumulations pull that all together here's the example from the last quarter of 2016 and you'll find the listings and they will actually give you a link to the individual publications as to where they exist on the state server just going back to the 1998 version where they broke out monographs in one section and serial publications in the later section and they interweave the things that were distributed and the things that were not distributed here's the fourth one down has the quick number listing and that's a classification similar to the su doc system it was created by Peggy Walker way back when she's retired 20 almost 20 years ago and a number of state depositories have adopted that we use that here at my university and so we're able to actually locate the documents by the agency that issued them and actually going back to the Florida public documents the first print version of the Florida public documents distribution and back then the first pages were all on white paper and were the things that were being distributed to you and at the back of the volume there was usually about the last quarter of the volume was on usually blue paper and these are the things they didn't get enough copies to send out to the depository libraries so these are the listings of things that were only available at the state library so this shows you the current distribution of the state depository libraries around the state so someone that's looking for a particular publication hopefully will be able to find a copy of it in one of these libraries that's switching over to searching the Florida academic libraries and you'll see that the 40 academic libraries public academic libraries in the state this will identify the 12 state universities which are the ones that have the outlined lettering and the 28 state colleges former community colleges in the state and so there's a joint catalog that you can search all of those at once and I would go here to search I'm going to search for the Florida visitor study as an example and I found six listings I'm going to open the first listing it shows me those locations at various in this case all but one of these is one of the state universities you'll notice some of them have that for example cod.e1 that FIU does that's that state depository system that was developed at Florida Atlantic University and I will necessarily have all of my collection Florida documents the historical documents may not be cataloged but if I find the listing here I can use the call number for one of those other universities and locate my copy probably under the same number since we've adopted that common system if I check availability you'll see FSU has it up through 2011 you'll find most of the libraries stop at 1996 in terms of their coverage you will see down at the bottom that UCF we we have our Florida documents collection through 96 and then our rosin campus we have 2001 through 2015 that's our hospitality school and so they made a point of picking up the later publications so if you question well why don't we have the later publications in the state library collection the state documents well we look here at the Florida visitor study at the state library listing and they've got 1981 through 2012 so we actually had more recent ones available in the state universities and they only have the 2012 edition electronically so what's happening here in terms of why are the others available I go back to the state university library catalog what I'll see is a link actually to the earlier version of Florida tourist study but I see a note here that says that after 1996 these were issued by the Florida tourism industry marketing corporation so what happened was in 1996 the the Florida Department of Commerce was abolished and their functions were largely privatized quasi-government agency and so they created the Florida tourism industry marketing corporation which became visit Florida fairly quickly and enterprise enterprise Florida and the focus of these two organizations initially was really on a marketing side of the house and they really stopped producing publications with data for researchers and the state of Florida it was really towards bringing tourists to the state and bringing businesses to the state enterprise Florida took quite a while to actually produce data that would have been collected by the Department of Commerce the visit Florida org site is the site for their partners so here's the 2015 Florida visitor study but if you click on this you have to be logged in as a paid partner of the Visit Florida organization to actually see the documents and here you'll see a number of the other partner tools they're available to their marketing partners some really useful reports that are not available generally not available in Florida libraries and in the state depository system because they're being produced by the quasi-governmental privatized Visit Florida tourism agency it's still the official tourism function for Florida but it's no longer a state agency as such so that's visit Florida org VisitFlorida.com will is intended for tourists in terms of located beaches and places to stay and things to do in the state but there's no data about Florida presented on the commercial site so on VisitFlorida.org you had to actually be a subscribing member or their partners to get access VisitFlorida.com you don't get that data reported but they do have a media site so this VisitFlorida.com they do have extracted information from those research reports so you can find some of the data here on a publicly accessible site Enterprise Florida has a lot more information that's publicly accessible so initially they had virtually nothing when it first happened in 96 you could not get any of the reports that you used to get from the Department of Commerce but they do have a data center that actually provides quite a bit of information these days so I do recommend that if you're looking for information about businesses in Florida there's quite a bit of information there so I identify some of the digital collections where you might look for information in Florida so we already talked a little bit about the Florida public documents collection which you have to access through the Florida State Library catalog there's also the Florida memory project that's run out of the state library state archives the Palm project is actually a joint publication or joint project from universities around the state of Florida University of Florida has quite a few digital collections and then there's the Florida electronic library Florida on Florida collection so the Florida memory project has really a lot of primary source material they also have a classroom center for helping teachers use the primary source material with their students and have lesson guides lesson plans the Palm project publication of archival library and museum materials for the state universities has a number of specialized digital collections the aerial photo photography collection the Everglades digital library has a lot of federal and state resources as well as some commercial sources Florida environments online has again federal and state publications the Florida geological survey Florida heritage collection has a lot of private commercial sources as well as some state publications and Florida historical legal documents does provide ditched as copies of a lot of the early legal materials from the 1800s the Florida heritage collection you can search full text of this but I've tended to fight if you don't know what you're looking for you may get a lot of zero results for things that actually exist there because you weren't quite searching for it correctly so I have listed here some of the publications that you could actually look for and I would look for these by title so they have a number of the digital publications here from various state university digitization projects some of these are available on the state library catalog and in some cases may be a longer run of digital copies than what's found here I know the state board of health and reports there's a longer run that's available through the state library but this is one of those places to locate these the report of the board of control right now the only the 1948 through 1952 digital copies are available my university digitized the the entire run from 1916 through 1968 but in the transition to a new platform they are temporarily not visible on the site they are working to get those established the Florida advancing state was produced in 1928 and gives you a early from 1907 through 1927 snapshot of the change in Florida as it grew over those years and there were special sentences died in the state of Florida on the five-year mark and three of those are actually digitally available in the palm project there's also the Florida history and heritage collections at the University of Florida one of those is the publications of Florida National Guard we have this in my collection here's one of those that I did a copy for a print copy but these do exist digitally they're very similar Indian Wars back at the very beginning here's one of the pages out of one of those where it's the actual descriptive the muster role of the members of that particular unit and provides all sorts of interesting geological historically information interesting information about where they were born and how tall they were and what their color of complexion I had here what they occupation I know one of these was a cow driver if they brought a horse with them what the value of that horse and the horse equipment was in terms of other places to look for information besides these starting places we identified the Florida documents index is one of those developed by Florida Atlantic we're going to go through each of these are the next slides so far documents index you can search by keyword and this identified for example keyword search for women identified the specific publications and identifies which agencies were issuing those publications if I actually opened that first entry what I'll find is this holdings location at the Florida Atlantic University the AG dot B2B colon D46 slash year that's that state agency call number system that was developed at Florida Atlantic and I could use this to locate those volumes I would also search through the Florida State Library catalog that this is one other way to get to these and we're stricting here specifically to those things that were distributed as part of the depository program there's also the go to our project that Daniel Cornwall from Alaska got started and it's a lid guide maintained by ALAs go to work of state agency database listings state by state here's the listing for Florida and will provide you with links to specific database resources maintained by state agencies another place to look for government information would be the various guides that have been created by depository libraries in the state of Florida this particular page will provide you with links to a number of those guides now some of these may not focus specifically on state publications but it's another place to look and see what information they've got and if you're in the state of Florida looking you can see a guide for a university near you that might have resources for you at the University of Florida the PK young library is the historical Florida history library special collection and their holdings are included in that state university state college catalog but there are parts of their holdings that are not available there they still maintain a card catalog that has a significant amount of additional information we actually have in 1977 they created a book form of that catalog and reproduce the catalog cards 21 cards per page let's look at one of those pages it's in four oversized volumes and there are 60 over 60,000 cards from the card catalog that are reproduced in that print source and I have used it to browse for material that may no longer be showing up or may not have been included in the state catalog for the PK young collection a couple of other things that might make use of it particularly looking at the early years the surveys bibliography is a four-volume set they are very large and very comprehensive they've tried to make an effort to scour various collections wherever they can go including out of state to identify every publication that covers Florida whether it's a official publication from the state of Florida or if it's commercially produced or if it's something a congressional document also looking at the Florida handbook by Alan Morris and distinctions of these things as a search tool and newspapers so I look at this bibliography of Florida they've actually got it's arranged year by year so I'm looking right now at the 1909 year and I moved into it to the Florida pages where they start a new section called Florida official publications and they group them so normally they're reached by the author of the publication all of the Florida official publications are grouped alphabetically by the author under Florida they also have listings for the US congressional publications the Congress publications the Florida handbook was produced by commercially produced by Alan Morris started in 1947 and produced through 2012 and although it's a commercial publication it's probably one of the best ways to look at the structure of Florida government as it changes over time and give you a clue as to oh that agency is now called this in 1955 the Florida handbook is available digitally in the palm collection so you can search through the Florida handbook by title and find the digital copies of these even though it was commercially produced it has been digitized there and the problem with these is they were produced every two years and they are different from year to year extremely different in some cases and what I found is you know like the 1950s they need to have a title page or a table of contents they just launched right in but you find all sorts of really interesting information in these he would change it up every two years so you'll find pictures that lasted for several years and then they disappear and never to reappear the early volumes do have an index of the illustrations and starting a page there's about 40 pages of boards and conditions pages that break out and they provide quite a bit of detail about the history of these organizations including the whoever was in charge they give a little biographical sketch of those particular individuals I mentioned dissertations of search and I would be searching through probably our university's uh indexes commercial indexes of dissertations I want to point out here's a bibliography that was produced I think back in 1977 as well and it tried to pull together all the doctoral dissertations over a thousand doctoral dissertations that dealt with Florida and arranged them by subject areas so it is something that does exist for browsing a lot of times a dissertation or a thesis the graduate student will have researched into some of these hard to find locations of materials of publications and state records and they give you a clue as to oh that's where you'll find that particular information so just takes to be a real good clue for how to start a research process I also use newspapers a lot I would typically go into our commercial subscriptions uh for current newspapers but there are several other projects the Florida digital newspaper library from the University of Florida has digitized quite a lot of the early newspapers there's also the Google newspaper archive that does have a several newspapers around the state of Florida that do go fairly current up through 2008 I would generally use the safety times in this particular listing but they do have the Daytona beach Daytona beach paper Sarasota paper also the Crawlick America site they've got some issues mostly from that Florida digital newspaper library but other sites you can get to these things the Florida digital newspaper library again a little bit finicky in terms of searching but you can search the full text of these I pull it up here an article from the weekly newspaper from the state of Florida they are digitizing current weekly newspapers from around the state quite a bit here's the Lake City reporter and here's how we use this as a fighting tool here's talking about a particular project school district project that's being coordinated through squatty river water management districts now I know what agency is doing this they've been doing it for the past seven years I now have names of this article of specific individuals at that agency so I was trying to find more information about this project and its history and resources for it I might go to that agency directly I'm going back here to 2007 I've actually got four different aspects of state government covered in this particular issue of the paper the one that's cut off on the left is actually Governor Broward had proposed that the drain the everglades and a number of the papers had been sort of poking fun at him for this and he tried to get the state legislature to enact restrictions on the press because he didn't like how he was being covered here we are 110 years later and sounds like some of what's going on now the middle article is talking about uniform adoption of uniform textbooks in Florida and some of the issues with that what I actually want to focus on is the last one where it's talking about a particular report from the commission to inspect the state and state of silence and it tells me that the report was actually included in the senate journal I'm looking at April 3rd as a date and I go to the Florida Senate website their archive site and pull up at the April 3rd journal of the Senate and I find here's the beginning of that eight page report the entire text of that report is actually included in the journal of Senate but I would never know to look for that if I hadn't had that article telling me here's where that report exists looking at title the presentation government the sunshine state looking at sunshine laws in terms of open records laws public records open meetings here's some of the resources that I will use quite often the government of the sunshine man manual is actually made available through the attorney general's office and it's jointly produced by the attorney general's office from state of Florida and by the first amendment foundation the office of open government site maintained by the Florida governor has opened and closed over time but it's currently available it does provide some information breakfast center at the university Florida school of journalism has quite a bit of information in terms of looking for open government resources they are the middle of changing to a new website and at the moment the only material that has actually migrated to the new site is the attorney's fees database but I communicate with them last week and they are at the process of migrating these other resources over to the new website first amendment foundation produced this far to public records handbook back in 1999 so it's quite old it is out of print but if you can't get your hands on it it's really really useful for figuring out how to look for specific type of information in Florida the entries on it talk about where to look why it's useful it provides tips and uh tricks in terms of how to look at the information how to locate it it also identifies what key pieces of information that are available in these public records and so you can see in terms of the listings of the different types of reports that are made available that are described here in terms of locating information around the state so this will pull the table of contents here to give you a feel for what might be out there and how to go about looking for it again this is out of print but if you get a hand on a copy it is really useful in terms of government accountability the opaga office out of the state legislature provides some really good reports the auditor general site again provides great resources in terms of finances and performance audits of state agencies and local government agencies and then the transparency Florida site the you can look by agency or by topic if I click on the consumer protection and insurance link here I actually find that there are multiple agencies in the state of Florida that may have specific programs that I might be looking for information detailed information about those programs at the auditor general site I can look by audit types I'm looking for performance audits operational performance audits I can restrict to that I can look by if the audits I can look at just state agencies or look at smaller organizations within the state the transparency Florida site gives you a lot of different types of resources in terms of includes for example the salaries of state employees including state universities all public record in terms of statistical sources I will often use Bieber the Bureau of Economic and Business Research of the state of Florida I'm sorry University of Florida the farthest district abstract that they produce is really useful similar to how you might use the statistical abstract of the U.S. in terms of fighting a table there and they're looking at the footnotes to see which agency which publication provides more information unfortunately I just saw recently that the their website as of the middle of December they are not getting the funding to continue to provide some of the data they've been providing on the website free so there's going to be charges to access their information after middle of December the state legislature maintains the Office of Economic and Demographic Research which has great resources in terms of statistical data also the Florida charts from the Florida Department of Health so here's just a quick page from the farthest statistical abstract you can see very similar to the statistical abstract with the U.S. the Economic and Demographic Research Office of State Legislature has a lot of really specific statistical data sources for you very current here's one of those the local government financial information handbook which actually will give you county by county tax distributions for all the special taxes that are in fact around the state mobile home licensed tax tells how much each county gets for their share of that revenue the Florida health charts site I'm looking at the behavioral risk factor data and you can actually select specific categories of health data and then get county by county listings and some nice graphs from that if I look at statutes getting back to our dates rush through these last ones 1927 is generally the compilational laws that I would go to most but there are digital copies of some of those earlier versions 1941 is when you actually start seeing Florida statutes continuously produced and you'll see them at the odd-debrate years up through 1969 because the state legislature did not meet they only met the odd-debrate years so you only got new statutes every odd year we had new state constitution 1968 that reconfigured a lot of things with the state so major watershed point in the state of Florida and the state statutes started to be reducing every year but until 1998 you had an odd-debrate full statutes multi-volume and a single-volume supplement for just the changes of the even-debrate years point out something in the foreign statutes index looking alphabetically after sheriff and shopping carts there's an entry for short titles has over 700 entries if you're looking for something like Baker act or the state living law which is actually the motor vehicle warranty enforcement act I would probably look online but this will give you those other entries the index to special local laws actually gives you those details of the laws that established specific cities I think know the reedy creek improvement district law which was established in 1967 it's actually the 1960th law that was passed that year and it established Walt Disney world but you have to know that it's reedy creek improvement district actually located there state legislature meets essentially in the spring it used to be every other year now it meets for a 60-day session starting in March it meets on the first Tuesday after the first Monday because it used to be in April and they didn't want to start the legislative session or April Fool's Day online sunshine is a great website for the state of Florida in terms of location information one of the notes in terms of looking for bills as of 1990 even number bills are Senate bills odd number bills are house bills so for example if you look in 2017 you will find that the bills only go up even number through 1026 so there is no even number bill 3692 because odd number bills are house bills and there's a lot more house bills introduced here's that online sunshine site state of Florida restructured completely so the statutes in the state of Florida changed from chapter 200 to chapter 1000 and there was no tracking back to those also the state organization of higher education governing they did away with it in 2001 and got reestablished through a political initiative by senator Graham to reestablish the board of governors which will have a gap there and so there will be here's looking at the 2016 foreign statutes for school districts and it looks like there's only goes back to 2002 you actually have to look at the 2001 foreign statute to see the earlier history of that same language interesting thing of the state of Florida we're just getting interesting things really quickly here the state secretary of state reports include things like the automobiles that were registered to the state here's rewards that were offered in the state lots of interesting things with these old volumes the Florida library collection actually has those auto registrations digitized in it special tax districts make up a huge part of the state you can actually look at all of these and find listings for them on the the special districts list site department agriculture maintains the charity list and you can actually find the 990 forms available for specific charities but you have to sort of know which one you're looking for state transportation does a interesting you can actually see the the traffic cameras across the highway see what accidents are construction projects that sort of thing writing up I would go to the ask a librarian service for the state of Florida which is the state library actually maintains a lot of the responding response to that but there are a lot of other libraries participating responding if you're a librarian in the state of Florida you can contact other depository librarians for help there's two lists that are maintained down the state library and at the University of Florida need to contact them about how do I get on that distribution state library has done some webinars for state agencies but I've got links here for some of those that give you some links and here is the follow-up information for contacting me and for a link to that research guide where I will be putting all of the listings for links to all the resources on there and that concludes the webinar back to you Ed. Thank you very much.


Welcome to the MOOC course on Interactomics. Proteins are dynamic molecules which interact with a wide variety of biomolecules such as lipids, nucleic acid and various small drug inhibitors, metabolites and many other biomolecules to provide different type of significant information for physiological action. Proteins also interact with one another to form the large complexes. These complexes regulate various fundamental processes such as signal transduction and gene regulation. A detailed understanding of protein interactions provides an opportunity to understand the protein functional behavior. Today in this lecture I will describe what is interactomics and a historic perspective of the conventional techniques involved in studying interactions which includes yeast to hybrid and immunoprecipitation techniques. Let us first start with interactomics. Interactomic comprises the study of interactions and their consequences between various proteins and other cellular components. The network of all such interactions known as interactome which aims to provide better understanding of genome and proteome functions. Interactions can have different kinds of impacts. It could be positive as well as negative. The one which is shown here in the slide shows the negative impact. Biology has evolved several mechanisms that regulate interactions such as post-translation modifications and presence or absence of activator or inhibitor molecules. Interactions can also be modulated by altering the expression level of proteins. The aberrant interactions such as the one shown in the slide can lead to the dysregulation of cellular functions and ultimately diseases such as cancer may happen. Proteins interact with a variety of biomolecules. The interaction of proteins with another protein or other biomolecules help to perform these functions. These proteins after interactions form complexes that modify the proteins help in protein transportation as well as many properties in which they are involved. The field of interactomics aims to identify the function of uncharacterized proteins so that one can assign the new role of various proteins. The mechanism to regulate protein activity can also be understood by studying the interactions. The network of protein interactions provide very valuable information for the processes such as signal transduction and various type of pathways in which these proteins can be involved. There are different methods of studying interactions because interactions occur in various ways. There are few interactions which are very transient that will be very short duration whereas other interactions could be permanent which may alter the activity. Few interactions are very weak whereas other interactions could be very strong. Interactions can also be obligate or non-obligate. They can form homo oligomers as well as different kinds of oligomeric units which could combine to form the hetero oligomer. This just gives you a glimpse of the complexities involved in the interactions because studying the interaction require various type of technologies just because of the sheer diversity of the interactions as well as different type of complexities involved. So, what are the physical reasons for these interactions? It could be electrostatic, hydrophobic, hysteric or hydrogen bond etc. Protein-protein interactions have various potential effects. Few effects are described in the slide such as catalysis, protein inactivation, altered kinetics, alteration of substrate specificity for substrate binding, new binding site information etc. These are just a few examples of potential effects of protein-protein interactions but they alter magnitude of effects which ultimately lead to either positive effects or dysregulation which may result in various diseases. These proteins are involved in many binary interactions and understanding of the underlying biochemistry of proteins and biological interactions is critical element for the development of novel therapeutics and diagnostics. Protein-protein interactions have various potential effects. Some of these effects are described in the slide. They alter magnitude of effects which ultimately lead to either positive effects or dysregulation which may result into various diseases. So, protein interactions are very essential for any cellular mechanism whether to discuss about signal transduction, DNA replication, translation, cell cycle control, how the metabolic processes are governed, the modality, how the growth and morphology are altered, splicing, transportation etc. I am sure if you think about your biological questions of interest you will be able to add many more examples here how protein interactions are essential for the activity of the cell. So what is significance of studying protein interactions? These are very dynamic which interact with a wide variety of biomolecules as I discussed previously such as lipids, nucleic acids, small drug inhibitors and many other biomolecules. Proteins also interact with one another to form the large complexes which regulates signal transduction and gene regulation. Let me now describe you few concepts involved in the intractomics field by showing you this animation. Interaction studies of proteins with various biomolecules help in deciphering and understanding the functions of various proteins in the complex network of cellular pathways. Proteins interact with other biomolecules such as nucleic acids, lipids, hormones etc. to execute a multitude of functions in living organisms such as signal transduction, growth and regulation and metabolism. These are few examples there are many other cellular processes and functions in which these are also involved. Protein interactions with other biomolecules can be of several different types. They may be weak or strong, obligate or non-obligate, transient or permanent. The physical basis for these interactions include electrostatic, hydrophobic, steric interactions, hydrogen bonds etc. So now let us talk about different methods to study the protein-protein interactions. Understanding the protein-protein interactions provides important clues to the function of proteins. The identification of interactions with known proteins may suggest the functional role played by a novel protein. There are two broad ways of studying this protein-protein interaction. One is the traditional approach and then there are few high throughput approaches. The traditional approaches rely heavily on yeast to hybrid, affinity chromatography and immunoprecipitation. The more recent studies include high throughput approaches such as protein microarrays and different type of label-free biosensors. The traditional way of studying protein-protein interactions involved yeast to hybrid and immunoprecipitation. These are two widely recognized technologies which have been used to map the protein-protein interactions on a large scale. These are yeast to hybrid also known as YTH and IP with MS also known as IP MS technique. Both of these approaches have been used to identify thousands of novel interactors in different organisms including human, C. elegans, drosophila, etc. Let us first talk about immunoprecipitation technique. IP or tandem affinity purification also known as TAP is a technique used to purify the protein complexes and study the protein-protein interactions. Depending on the protein that need to be purified, different types of tags could be attached to the bait proteins. Through the immunoprecipitation method, the purification of protein complexes by IP or tandem affinity purification method is performed. The target proteins and its interacting partners are isolated from a given complex sample and then by using different kinds of tags, these proteins can be isolated from a mixture and then further processed which I will describe in the next slide. In IP method, the antibody specific to the bait is attached to the whole cell extract which forms the complex. Remember, this step is performed in the native or non-dening condition. The protein-protein complex is immobilized on protein A or protein G c-pherose beats. Protein complex is eluted and further analyzed on SDS pages. As you can see on the slide, it shows that the antibody is used which binds with the antigens and the unknown ligand and this complex is separated on SDS pages. This condition is done under denaturing condition whereas the first step was performed under non-denaturing conditions. What are the merits of using IP method? In this method, proteins are isolated in the native state. Why native state is important? The native state will allow the native complexes to be formed. It also allows the formation of post-translational modifications. It is essential to perform these steps in the native or non-denaturing conditions. Interactions by using the IP method are natural and by using these methods, large-order complexes can be observed because the native state will promote the native complex formation. What are the different demerits of using IP method? It has been reported that frequently, sticky proteins may be picked up. The proteins which are not specific, they could also be eluted out because they are sticky on the other protein surface. It means few proteins that are specifically interacting with target of interest will be isolated along with non-specific sticky proteins. It is unclear whether this interaction is direct or indirect because the proteins which are directly interacting will bind along with those indirectly bound interacting proteins will also be eluted. This method is also quite expensive because it needs a good equipment setup and different type of analytical and computational analysis. So, let us discuss the immunoprecipitation method, how the mechanism occurs in more detail by showing you this animation. In immunoprecipitation, the protein of interest is fused with a tab-tag which contains a Cal-modulin binding peptide, a TeV cleavage site and protein A. Depending upon the proteins to be studied, this tag can be modified. The tag is then bound to a column through affinity interactions between the protein A and IgG. The protein mixture whose interactions with the braid protein are to be studied is then added. Some of the proteins form a complex with the braid protein through specific binding interactions. The remaining unbound proteins are then washed away. It is followed by cleavage at the TeV site by the TeV protease to release only the protein complex bound to CBP. These reactions constitute the first affinity step. In the second affinity purification step, the braid pre-complex is bound via the CBP domain to a Cal-modulin functionalized column in the presence of calcium ions. The column is washed to remove any other unwanted contaminants after which the keratin agent is added which complexes the calcium ions. Once these are removed, the CBP-Cal-modulin interaction is weakened and leads to the release of purified protein complex. Once the protein complex has been purified, the components of the complex are separated by electrophoresis under reducing conditions. The LGS gel is then analyzed and the protein components evaluated thereby providing an understanding about the interactions with the braid protein of interest. So we have discussed the traditional ways of studying protein-protein interactions. First one was immunoprecipitation. Now let us move on to the next method which is yeast to hybrid. In yeast to hybrid, two types of proteins are involved, a braid protein and a pre-protein. Bade protein is protein of interest whose interaction is to be studied. The Bade protein is fused with the binding domain of the transcriptional activator by inserting it and expressing along with the binding domain in a suitable manner. The pre-protein is a protein whose interactions with the Bade needs to be determined and that is also known as pre-protein. The pre-protein is fused with the activation domain of the transcriptional activator. So the successful interactions of the Bade and the pre-protein activates the transcription of reported genes. The yeast to hybrid system was first demonstrated by scientists Fields & Song in 1989 for studying the protein interactions. Since then, this approach has been widely used in different organisms for different type of biological questions to be addressed. In general, the yeast to hybrid system uses the Bade binding protein and the pre-activation domain. These hybrid proteins are jointly expressed in the yeast nucleus. If the protein interactions established between the Bade and the pre which are coupled with BD and AD, then transcription will occur. As you can see the slide, the transcriptional activation for the two hybrid system consists of two protein domains. One is DNA binding domain which remains bound to the promoter region and is fused with a suitable Bade protein whose interaction with another protein is required for the study. The activation domain is the other domain of the transcriptional activator which is fused with the pre-protein. This activation domain when bound to the DNA binding domain forms the functional transcriptional B-activator and brings the expression of reporter genes. As you can see in the slide, the Bade protein binds with DNA binding domain and the partner protein or the pre with transcribe activator domain or AD domain. Once the BD binds with DNA, while the AD activates the transcription when both of these are associated, the Bade with the BD and pre with AD when they bind together the transcription event occurs. What are the different advantages of using yeast to hybrid system? As I mentioned, since 1989 when it was shown first time studying the protein interactions using yeast to hybrid, it has been used for various type of interactions including protein RNA, protein DNA, analysis of particular complexes as well as studying the large protein interaction networks. The protocols for yeast to hybrid is quite simple. Unlike the other approaches, there is no requirement for heavy instrumentation here. The method also allows to screen the large libraries very rapidly. What are different disadvantages of using yeast to hybrid system? It provides very high positive and negative rates. So high false positive rate has been one of the major limitation of this approach. The protein must localize and interact in the nucleus. So that is the limitation of the approach because it is restricted to the binary interactions or if you compare with immunoprecipitation, the complexes cannot be formed. Soplication in a non-east environment is also questionable because the system mostly used here is yeast. The protein folding if you are aiming for studying the mammalian system is not guaranteed. It is also not quite sensitive to the toxic genes. Finally, it is limited to the pairwise interactions. If you have fair idea about the molecules which are potentially interacting then you can use the pairwise study by using yeast to hybrid. But also if you just want to generate a list of potential interactors which may or may not be true then those can be quickly screened by using different libraries by using yeast to hybrid method. Once you have established the list of potential interactors then you can use different type of validation approaches for really establishing how many of these proteins are interacting well and how many of these are false positives. So the yeast to hybrid screening remains as a novel biological technique which can be used for screening and the discovery of protein-protein and protein other biomolecular interactions. Let me show you how yeast to hybrid works in the following animation. We will not talk about yeast to hybrid. In yeast to hybrid screening the binding of the transcriptional activator protein composed of binding domain and activation domain to the promoter region is essential for expression of the corresponding reporter genes located downstream of the promoter. The binding domain is fused with the bait protein while the activation domain is fused with the prey protein. Binding of either one of the fusion proteins to the promoter is not sufficient to bring about transcription of the gene. The two hybrid screening protocol uses this interaction as a basis for the screening protein interactions. When the bait protein bound with the binding domain interacts with the prey protein fused with the activation domain there will be expression of reporter gene which can be easily detected. LAXZ is a commonly used reporter gene whose protein product beta galactosidase leaves the substrate at scale resulting in blue color colonies. Let me show you the yeast to hybrid concept applied in one of the studies by showing this interaction. To study the protein-protein interaction between the vaccinia virus and human proteins in this study authors used the virus bait proteins fused with the C-terminus of Gal 4 binding domain BD while the prey human proteins were fused with Gal 4 activation domain of the transcriptional activator. These interactions were further validated by other techniques which gave a successful validation rate of 63%. Let me show you by this interaction how this yeast to hybrid screening can be used. If you drag and drop the components such as the transcription will be carried out you can see the first Gal 4 domain are here to the promoter region and now as soon as the activation domain binds their transcription events happen. So in summary today we talked about significance of studying interactions in the field known as intractomics. We discussed different types of traditional approaches which have been used for studying the protein interactions such as yeast to hybrid and immunoprecipitation assays. In the next lecture we will discuss a variety of platforms currently being used for studying the proteins in the high throughput manner by using the exciting field of protein microarrays. I will describe to you how different contents such as antibodies, verified proteins, peptides or even cell-free expression could be performed to make the protein microarrays. Thank you.


Hey guys, welcome to another video for my creative year, albeit a little late again. For those that are unaware, I'm having some dental issues, so anyway. If you didn't see my video from the beginning of October 2020, then I will link it below in the video description, along with a tutorial for last month, because I got the prompts confused for for September and October. So last month was supposed to be bright colors. This month was supposed to be monotone shades of one color. I got my prompts confused, and last month I did a tutorial and the introduction video using the monotone prompt, and this month, yeah, so anyway. So I've already done a monotone tutorial, so I'll link it below, along with the October first video.  Maybe I might throw some green in there somewhere. The list of the prompts and color suggestions for the month should be in the photo album for October. There should be a photo album for 2020 in the My Creative Year Facebook group, and it should have all of the prompt lists for each month going forward. Each day of the month has a different word suggestion, prompt for inspiration, and then there's usually a theme for the month, so use them or not. Hopefully some of them inspire you. In the meantime, I have this scrap piece of watercolor paper that I obviously was trying to do a watercolor chart on for a palette I put together, which I totally screwed up the lines on, so I did it over again. This is just a scrap piece of paper, but I'm thinking I want to paint over it, use this as texture, and create something I can cut up into maybe tags or postcards. I've got a couple of stamps from my Etsy store, including Modern Atomic 14, 13, Modern Atomic 13, and Modern Atomic number two. So we're going to use my new jelly gouache palette and these stencils and probably some colored pencils and things to create something interesting. I've got this one double-sided pencil that I'm really enjoying a lot that I found that I have that is by Carandash. It's a by color 999 pencil. It's a blue on one end and red on the other, and it is a lot of fun. So we're going to get to it, and I'll be right back. This is just a quick little easy project and a project reminder that your art never has to be complicated. It can be just something as simple as a little mixed media postcard. I didn't use the yellow, but I did use the lime green, which is kind of in the yellowish family. But anyway, I think they turned out super cute, and they are sort of postcard card size, so I can save them to send off to people I'm thinking about or I can use the holidays are coming up. And because of COVID in the pandemic, who knows if we're going to actually be able to see anybody outside of our bubble. So if you can't see them, you can take one of these little cards and you can send it off to them with a little note on the back. Say, you know, hey, I'm thinking about you. I hope you're happy. I hope you're at peace. Hello. You know, whatever. It doesn't have to be complicated, but at the same time, these are super cute.  Don't forget to go out and do something nice for yourself because you deserve it. Support the free content in the Facebook art groups and the YouTube channel if you can. Use the links in the description below for my Etsy shop and all of that. And wow. Yeah, just try to keep your chin up and keep going forward. And that's all we can do. We're here for each other as always. All right. That's it for now. See you all later. Bye, guys.


Hey everybody, this is Christian Buckley with another MVP buzz chat and I'm talking to you with Fabian. Hello. Hi, I'm Fabian. I'm MVP from Germany since May this year. All the way since earlier this month. Congratulations. Yes. So what do you do? Where do you work and what's your role? I'm currently a cloud architect at Club Kenya, GAB in Germany and I mostly support clients working with Microsoft products about the Microsoft Defender suite. So everything from Microsoft Defender for endpoint to Microsoft Defender for cloud and everything that's related to that. And I say, so you're a cloud and data center management. What's encompassed within that focus area for MVPs? So I can only speak for myself because this area is very broad and cloud and data center is very, very big. Yeah, because you have a lot of people that are Azure focused and they describe themselves the same way and they're an Azure MVP. And so like I get the data center management side of it, but will you attach cloud? That could be a lot of things. Definitely. So yeah, mostly I think I landed in this category because of my focus on PowerShell and the security side. And since Microsoft has no security MVPs yet, I think it's a bit of a catch all everybody who has to do something with cloud lands in somewhere in between. And since PowerShell is one of my hobbies, I think that's the data center management part of it. Well, it's almost like that they could, I realize now there's a focus on GitHub specifically. And but this is kind of between that and this, it's kind of like the DevOps world. So it's all the operational controls, the traditional DevOps type activities. Yes, yes, for sure. Well, very cool. Well, so your brand new MVP. So what was your kind of journey to becoming an MVP? I'm always interested to hear like what your path was, how long you were trying to become an MVP if you were at all trying? Yeah, actually no. So it wasn't never my goal to get this title and I'm very honored to have gotten it still. Yeah, I think my path on the Microsoft horizon started in about 2008. In 2008, I visited the PowerShell conference in Europe in Hannover and was very hyped by the conference and everybody around there and started a meetup group after that with a friend of mine Christopher Burmeister and we both thought it would be great to have our own meetup in Hamburg related to PowerShell. So I started that and yeah, then I started to talk at conferences and other meetups. And I think one came to another and then I started blogging more. So I had a blog since I think about 2011, but initially I started a little bit for two years, only in German. And then like most blogs around this time, it's yeah, was mostly dust and nothing else. And in 2021, I started again with blogging regularly and tried to yeah, just write up what helped me or what I did find in my daily IT business and what I thought would be interesting to other people. And that's the same for stuff I talk about conferences and meetups. It's stuff I stumble upon and don't find resources that what I was searching for. Or even sometimes I use it just as a note for myself. So as a little notebook, okay, and I write up whatever I learned because sometimes they are great blogs already out there, but I have to do it myself. And after that, it's like a lessons learned. Let's write it up and maybe somebody comes up on some of my posts and find them useful as well. That's one of the things I love coming across when I'm researching something. And I find when people are writing about something that are kind of on the same journey, but maybe six months before me or a year before me. But they're doing the whole, the working out loud model where they're sharing their journey, what they've learned, resources that they pointed to. I love that style of writing and content and videos, of course, as well.   Oh, yeah, definitely. I like the follow-up Google results. Yeah. I like the follow-up. Somebody's posting like, yeah, I fixed it and never an explanation about how or what's even worse is when you have like a Microsoft person's like, hey, can we jump on the phone and I'll walk you through the solution? It's like, whoa, whoa, wait a second. You know, please share it publicly. I think that's something really great about the community and the Microsoft space and I think in other spaces as well, that there are many people trying to push their ideas through different things like Twitter, blogs, videos. And so you have the opportunity to find really great answers for problems out there. And sometimes if you find something where there is no information or not enough, then if you find the solution, please write it up. And I think that's the best thing you can do for the community at the moment. Yeah. Well, that's why it's so important to properly cite and reference the work. If you're answering a question that's out on tech community, then grab the link to that. Share it or go back into that post and say, hey, I just did a blog post. You get a lot of people that do that. I've done that. I'm sure you've done the same thing.  And then I do a walkthrough of this solution. And it's great to see the traffic if you pay attention to that stuff, where people are coming from and how much that comes through the forums is useful around that. I wish that the Microsoft, if a Microsoft person touches a question out in the forums, they should be required, if it's answered, if it's solved, to go back and post the necessary information. That should be a required answer. That would be great. Yes. Yeah. So I think Microsoft has come a long way in documentation. So today's docsmicrosoft.com is just awesome. Like 20 years ago, when I started in IT, it was like, yeah, there is some article, but maybe not so great. And they come such a long way and using GitHub as a method to really be able to say, oh, there's something wrong in this. And I just open a pull request and create as well. What's that? I don't know if people understand how much of that great content, because I agree. I think Microsoft made some structural changes. But one of the biggest differences that 20 years ago they didn't do was that they've tapped into the community to provide a lot of that content. So there's a ton of content that the world, they don't advertise the fact that, hey, this stuff was written by, not all the time, written by people in the community. Microsoft will even occasionally pay MVPs and other experts to go in there and write certain content that they know is a gap there. But then a lot of it is, like you said, it's managed through GitHub. People go and identify, hey, this isn't right. They'll go in and make a correction. They do a pull request, change the documentation, post it out there. So it really is. And there Microsoft is recognizing and rewarding people for those kinds of contributions, which is part of where the GitHub recognition comes from. Yeah, for sure. And I think that's something that is really showing how Microsoft just shifted like from 15 years ago in a whole other mindset. Community firsts and pushing products faster and all the product engagement through product groups and stuff like that. So that's not something you only can do when you are an MVP. So the customer programs are open for everybody who has an NDA as a company. And if you have, you should join because I think that's the best way. If you work with the products, get to know the product groups and get information right out of firsthand. Yeah. Well, maybe we should be more specific. Like where do you actually, where would you suggest people would go and sign up? Like my first thought would be like the patterns and practices team. I know there's renaming or whatever around that, but that's an area where you can go and find a number of different projects that might be of interest to you personally or your company and join in. Just start joining those calls. Yeah. And there's also the security guys over at Microsoft. They're very open in their roadmaps. So there are different, yeah, like connection programs you can join. I don't have the name at the moment. So but I, there are really great options there. And I think AKMS slash security, they have it listed somewhere around there. And yeah, as I said, it's not just for MVPs or a few chosen ones, but everybody who's able to figure out how to get an MBA. So most of the time just talk to the Microsoft representative in your company, try to figure out who that is. And they are more than happy to help you to get into such programs. One, of course, they also have like we've got bills going on. You know, we recorded a while back. This is it's actually happening this week while we're talking just kicked off today. But there are a number of different programs that you'll hear about in those sessions. Like one that I'm involved in is the it's the Viva customer connection program or partner program. So I think you could if anybody is interested in because there are customers of all sizes around the world, and they go through and they talk about what's coming with the product, they talk about deployment questions, they share stories and examples, it touches on for those that are in the modern workplace touches on into the different workloads that are involved with the solution. So, you know, SharePoint and teams and other things that are happening there. And I think you can I think it's just VCCP at Microsoft.com and you can reach out and say, hey, I'd love to join these calls. And so they're actively, you know, inviting people to go and join that. So there's a lot of stuff like that, depending on your area of focus, what you want to get involved in. Yeah, for everybody, everybody who's interested in the identity community, the identity advisors, it's a great jammer community out there, and also sharing information that is really, really top notch. And also, it's another level of interaction with other peers. As you said, it's not only that Microsoft product group members are there, but also other guys and girls that work in the field and doing doing this work and say, okay, I have this problem and somebody else is here. No problem. I have this yesterday. So let's figure it out. Well, I know that you're also very involved in the user groups, local user group stuff. That's another place to go get involved.  So that's a great way to get involved. Yeah, it's the local meetups. And I hope post COVID or I hope we are post COVID, it will get more from teams meetings to read meetups again. Because that's something it's really great to meet other people working in the same field in your city or around it. And to get a network by meeting those there, it's just great. And I think you just have to Google for your field of expertise and meetup. And you will find many user groups out there that are happy to have you. Yeah, that's the secret of is just getting involved. People say, well, how do I get started? I said, well, you find something and get started. And I'd say that you have a lot of people that will come out to one or two meetings and then just kind of disappeared. And then and then they wonder why, well, I never, I never got pulled into something I never really got involved. You know, and it's like, yeah, you have to be consistent, you have to participate, you have to I'd say even initially think about more about giving back than what you get out of that. It's inevitable that you are going to individually or your company have benefit out of you'll get some benefit out of participating in the user groups through the connections that you make, you might hear two or three months in a row topics that you're like, yeah, I'm aware of this stuff. And then suddenly there's topic like, wow, I've never heard about this. This is something that can add value. So you just need to be consistent and be there and be present. Yeah, I think one more really important thing is if you are already very experienced in one topic, don't be afraid to go to those meetings where somebody is explaining something more basic because in the end, the room is full of people who is interesting in this topic. And you will find people there that are on your level or maybe you can help people. So it's just a great exchange. And don't expect that meetups are always the newest of the newest and just the hot stuff. But they are there for everybody. And so they have to accommodate for that as well.  And my first response was, well, it's none of your damn business. It's my lunch. It's my time. What do you care? But the feedback was that I don't think that our team is getting value out of you participating in this user group. And my response was, I'm not so selfish as to think that I have to personally get value every time. I'm a lot of times I'm providing value by being there, by joining the conversation, by encouraging others to ask questions. There's value in being present at those activities, even if you're not passionate about that topic. It's important that we support others. There's a bunch of data that's out there that if there's a small group and no one's asking questions, then people are less likely to ask questions. That there's data around online, like forums and within user groups and conferences, that when there's somebody raising a hand, it's more likely other people will then raise their hand to ask a question. And so if you can do nothing more than be there, and if no one's raising their hand, then ask a question and kick it off. It will raise the value, the benefit of everybody else, to everybody else there. So, yeah, anyway, I'm off my soapbox around that. But yeah, I'm glad to see Microsoft is really embracing the user group model and doing a lot more support. And they keep hiring MVPs, a lot of MVPs. Yeah, I think we have to get the number lower to get new ones. Yeah, well, is that what it is? So there's so many old-timer MVPs that are kind of clogging up. This is like, let's pull them out of the system.  So we'll have to make that accusation the next time we're all together. But Fabian, really appreciate your time and getting to know you. For folks that want to follow you and find out more about you or get in contact, what are the best ways to reach you? You can reach me on Twitter. So DMs are open. Just write me there. It's Fabian underscore beta. Or you can visit my blog. It's cloudbrothers.info. And if you live somewhere near Hamburg, just within the Hamburg PowerShell user group, you're always welcome. Excellent. Well, we'll provide all the links, of course, on the blog posted on YouTube. Fabian is really great to be able to connect with you. And we'll hopefully see you with the next MVP summit once we're back in person. Yeah, I hope so as well. Fingers crossed.


The biosynthesis method is thought to be a clean, non-toxic and environmentally friendly way to produce silver nanoparticles. It involves using certain types of microorganisms to create these particles from a solution containing silver ions. These microorganisms can then be used to control the size of the particles, with smaller particles being more desirable due to their increased surface area and high antimicrobial activity. In this study, the fungus fusarium oxysporum was chosen because it produces the smallest particles when treated with specific concentrations of silver nitrate and incubation temperatures. This treatment also results in the highest antimicrobial activity against both ischoricia coli and staphylococcus aureus. Additionally, the cytotoxicity of the silver nanoparticles was measured and found to have an IC50 value of 121.23 gCm3. This article was authored by Sheriff Musa Hussaini, Tohera Esala and Hende Antia. We are article.tv, links in the description below.


Live from Las Vegas, it's theCUBE. Covering VMworld 2017, brought to you by VMware and it's ecosystem partner. Okay, welcome back everyone. Live CUBE coverage here at VMworld 2017. Three days, we're on our third day. VMworld, always a great tradition. Our eighth year, I'm John Furrier with theCUBE. Co-host with me, Dave Vellante of Wikibon and our next guest is Keegan Riley, Vice President and General Manager of North American Storage at HP Enterprise. Welcome to theCUBE. Thank you, thanks for having me. Thanks for coming on. Love the pin, as always, wearing the flair. Love the logo, I always comment on that when I first was skeptical on it, but now I love it. But HPE doing great in storage with the acquisition of SimpliVity and Nimble where you had a good run there. Absolutely. We just had a former HPE entrepreneur now on doing a storage startup. So we're familiar with the HPE storage. Good story. What's the update now? You got Discover in the books. Now you got the Madrid coming up event. Software defined storage. That pony's going to run for a while. What's the update? Yeah, so appreciate the time. Appreciate you having me on. The way that we're thinking about HPE storage, it's interesting. The company is so different. I mentioned to you guys when we were talking before that I actually left HPE to come to Nimble. So in some ways, I'm approaching the gold pin for a 10 year anniversary at HPE. And they retro that too, you get that grandpa out of it. Absolutely, vacation time carries over, it's beautiful. But the HPE storage that I'm now leading is in some ways very different from the HPE storage that I left six years ago. And the vision behind HPE storage is well aligned with the overall vision of Hewlett Packard Enterprise, which is we make hybrid IT simple, we power the intelligent edge, and we deliver the services to empower organizations to do this. And the things that we're thinking about at Nimble, and the things that we're thinking about as kind of a part of HPE are well aligned with this. So our belief is everyone at this conference cares about whether it's software defined, whether it's hyperconverged, whether it's all flashed on and so forth. But in the real world, what clients tend to care about is kind of their experience and we've seen this really fundamental shift in how consumers think about interacting with IT in general. The example that I always give is, I've been in sales my whole career, I've traveled a lot and historically 15 years ago, when I would go to a new city, I would land and I would jump on a airport shuttle to go rent a car, and then I would pull out a Thomas guide and I would go to cell C three and map out my route to the client and things like that. And so I just expected that if I had a meeting at 2 p.m., I needed to land at 10 a.m. to make my way there, that was just my experience. Cut to today, I land and I immediately pull out my iPhone and hail an Uber and reserve an Airbnb when I get there and for a 2 p.m. meeting I can land at 1.15 and I know Waze is going to route me around traffic to get there. So my experience as a consumer has fundamentally changed and that's true of IT organizations and consumers within those organizations. So IT departments have to adapt to that, right? And so kind of powering this hybrid IT experience and servicing clients that expect immediacy is what we're all about. Right? So I love that analogy. In fact, when we were at HP Discover, we kind of had this conversation. So as you hailed that Uber, IT wants self-driving storage. Absolutely. That's good. Bring that, tie that back in, because we talked a lot about that in kind of a colorful, joking way, but that is the automation goal of storage, is to be available. We talk about edge on structured data, moving compute to the edge. It's nuanced now, storage and compute, all this is working together through software. Self-driving storage means something, it's kind of a joke on one hand, but what does it actually mean for an IT guy? No, it's a great question and this is exactly the way we think about it and the self-driving car analogy is a really powerful one, right? And so the way we think about this, we're delivering a predictive cloud platform overall and notice that's not a predictive cloud storage conversation and it's a big part of why it made a ton of sense for Nimble Storage to become a part of HPE. We brought to bear a product called InfoSight that you might be familiar with. The idea behind InfoSight is in a cloud connected world, the client should never know more about what's going on in their infrastructure than we do. So we view every system as being at the edge of our network and for about seven years now, we've been collecting a massive amount of information about infrastructure, about 70 million telemetry points per day, per system that's coming back to us. So we have a massive anonymized data set about infrastructure. So we've been collecting all of this sensor data in the same way that say Uber or Tesla has been collecting sensor data from cars, right? And the next step, kind of the next wave of innovation if you will is, okay, it's great that you've collected this sensor data, now what do you do with it, right? And so we're starting to think about how do you put actuators in place so that you can have an actual self managing data center? How can you apply machine learning and global kind of correlation in a way that actually applies artificial intelligence to the data center and makes it truly touchless and self managing and self healing and so on So that vision alone is when, I'm sure when you pitched that to Meg, she was like, okay, that sounds good, let's buy the company. But as well, there was another factor which was the success that Nimble was having. A major shift in the storage market and you can see it walking around here is that over the last five, seven years there's been a shift from the storage specialist, expert at managing LUNs and deploying and tuning to the sort of generalist because people realize, look, there's no competitive advantage. So talk about that and how the person to whom you've sold in your career has changed. Yeah, no, absolutely, it's a great point and I think it's, in a lot of ways, it goes to, you're right, obviously Meg and Antonio saw a lot of value in Nimble Storage. The value that we saw as Nimble Storage is as a standalone storage company with kind of one product to sell, there's a saying in sales that if you're a hammer, everything looks like a nail and so it's really cool that we could go get on a whiteboard and explain why the castle file system is revolutionary and delivers superior IOPS and so on and so forth, but the conversation is shifting to more of a solutions conversation. It moves to how do I deliver actual value and how do I help organizations drive revenue and help them distinguish themselves from their competitors leveraging digital transformation. So being a part of a company that has a wide portfolio and applying a solution sales approach is, it's game changing, right? Our ability to go in and say, I don't want to tell you about the Nimble OS, I want to hear from you what your challenges are and then I'm going to come back to you with a proposal to help you solve those challenges. It's exciting for our sales teams, frankly, because it changes our conversations that makes us more consultative. All right, talk about this, talk about the sale. Value conversation. Talk about the sales engagement dynamic with the buyer of storage, officially mentioned in the old days, now new days. A new dynamic is emerging, we've identified on theCUBE past couple days and I'll just kind of lay it out for you and I want you to get a reaction. I'm the storage buyer of old. Now I'm the modern guy. I got to know all the ins and outs of speeds and feeds against all the competitors, but now there's a new overlay on top of which is a broader picture across the organization that has compute that has edge. So I feel more, not deluded from storage, but more holistic around other things. So I have to balance both worlds. I got to balance the, I got to know and nail the storage equation. Okay, as well as know the connection points with how it all works, almost as an OS. How do you engage in that conversation? Because it's hard, right? Because storage, you go right into the weeds, speeds and feeds under the hood, CR numbers, we're great, we do all the stuff, but now you got to say, wait a minute, but in a VM environment, it's this, in the cloud, it's like this, and it's a little bit of bigger picture, HCI or whatever that is, how do you deal with that? No, absolutely, and I think that's well said. I mean, I think the storage market, historically has always been sort of, all right, do you want Granny Smith apples or Red Delicious apples, right? It always sort of looked the same, and it was just about, you know, I can deliver X number of IOPS, and it became a speeds and feeds conversation. Today, it's not just not apples to apples, it's like, do you prefer apples, pineapples or vacuum cleaners? Like there's so many different ways to solve these challenges, and so you have to take the conversation to a higher level, right? It has to be a conversation about how do you deliver value to businesses? And I think, I hear this- And it gets confusing to the buyers too, because they're being bombarded with a lot of fud, and then they still got to check the boxes on all the, under the hood stuff, the engine's got to work. And they come to VMworld, and every year there's 92 new companies that they haven't heard of before that are pitching them on, hey, I solved your problems. I think what I'm hearing from clients a lot is, they don't necessarily want to think about the storage, they don't want to think about, do I provision RAID 10 or RAID 5? And do I manage this aggregate in this way or that way? They don't want to think about it, right? So I think this is why you're seeing the success of some of these next generation platforms that are radically simple to implement, right? And in some ways, at Nimble, when we were talking to some of these clients who have sort of a legacy approach to storage, where you've got like a primary LUN administrator, there's nothing wrong with that job, it's a great job, and I have friends who do that job, but a lot of companies are now shifting to more of a generalist, I manage applications and I manage, you know. You manage a dashboard console. Exactly, yeah, so you have to make it simple and you have to make it, you don't have to think about those things anymore. So in thinking about your relationship over the years with VMware, as HP, you were part of the cartel, I call it, the inner circle.  That's a great question, and I remember the early days at Nimble when we were knocking on the door and they're like, who are you again, Nimble, who? And we're really proud of the reputation that we earned inside of VMware, they're a great partner, and they've built such a massive ecosystem, and this show is incredible, right? They're such a core part of our business. At Nimble, I feel like we earned a seat at that table in some ways through technology differentiation and just grit and hustle, right? We kind of edged our way into those conversations and performance, and we started to get interesting to them from a strategic perspective as just Nimble Storage. Now as a part of HPE, HPE was, and in some ways as a part of HPE, you're like, oh, that was cute, we thought we were strategic to VMware, now we actually are very strategic to VMware, and the things that we're doing with them from an innovation perspective, it's like just throwing fuel on the fire, right? So we're doubling down on some of the things we're doing around like VM vision and InfoSight, our partnership with VSAN on ProLiant servers, things like that, it's a great partnership, and I think the things that VMware's announced this week are really exciting. I think it- Thank you, great to see you, great to have you on theCUBE. Thank you so much. I'll give you the last word. What's coming up for you guys in HPE Storage as the Vice President General Manager?  What should customers look for from you guys? No, I appreciate that. There's a couple things. So first and foremost, our R&D budget just got a lot bigger, specifically around InfoSight. So you'll see InfoSight come to other HPE products, 3PAR, ProLiant servers, so on and so forth, and InfoSight will become a much more interesting cloud-based management tool for proactive wellness in the infrastructure. Second, you'll see us double down on our channel, right? So the channel, Nimble was always 100% channel, SimpliVity was 100% channel, HPE Storage is going to get very serious about embracing the channel. And third, we're going to ensure that the client experience remains top-notch. The NPS score of 85 that Nimble delivered, we're really proud of that, and we're going to make sure we don't mess that up for our clients. You know, it's so funny, just an observation. I worked at HPE for nine years in the late 80s, really 90s, and then I've been covering theCUBE for over seven years now. Storage is always like the power engine of HPE. And no matter what's happening, it comes back down to storage. I mean, the earnings, the results, the client engagements, storage has moved from this corner kind of function to really strategic, and it continues that way. Congratulations. Thank you so much, appreciate the time. All right, it's theCUBE. Coming up, Pat Gelsinger on theCUBE at one o'clock, stay with us, got all the great guests and alumni, and also executives from VMware coming on theCUBE. I'm John Furrier, Dave Vellante. We'll be right back with more live coverage after this short break.