So, I'm live now here at Hardware Sugar. Although mag-6pm na, we close at 4.30pm. Our regular shop hours are 9.30am to 4.30pm every day. I do this stream once a month just to kind of update you guys at may stock natin dito, showcase any cool builds that we have ongoing. Although, unfortunately, we don't have a lot of cool builds going on at the moment. But we'll get into that right after the ad break which I will do once I find the proper thing to press. Baraming na kaming na tulungan. Dati? Sa din di purta ko? But now, I'm from a log of my life. Dati, ingmira sa nolang purta sa ng life ko. But now, I'm a world-class Zumba instructor. So, web developer ako and content creator for a YouTube channel and ngayon ganun pa rin ako pero activated na yung windows ko. Kaya ako nagahahanap ka ng legit, mura, at original software. Check out cdkoffered.com Check out cdkoffered.com Check out cdkoffered.com Stop ITX addict, stop ADN, rainy eve, is it raining outside? I got to the shop around 30 minutes ago it wasn't, actually, my eat it nga. I didn't notice, at least here in Makati It's been quite hot the whole day, actually. Stop, Anton. Yes, actually, we'll get into that. It is quite messy. I would swing the camera around. I'll show you the mess in a little bit. We've managed to confine it to one side. Well, this is good. We got a, it is messy as you intuited kasi we have a big order. But, I mean, they're office computers so they're not cool. I mean, they're not enthusiast level. But, they did order in bulk. Actually, this customer already ordered from us before. They ordered from us very early on in the shop history na sa Gijo, sorry, not even Gijo, na sa Buendia pa kami when they did their first order with us and then I guess they needed an upgrade four years later so they came back and so we'll look at the, we'll look at the, we'll look at the dump. I mean, it's like we tried to make it as neat as possible but we've shunted off everything for those bills off to one side. Subgamer zone, sub makoy. Yes. The GPUs they have are 610, no, they're not yet 1630 Bion or something. I'm a bit embarrassed. I'm actually the one who came up with the bill but no, I can't quite remember any specs in that. Intel 10th Gen, H5 something mobile. Anyway, we'll talk about it in a little bit. No, no, we don't want to benchmark the, I don't think I would want to benchmark the office bills although, I will discuss them in a little bit now even if they're just office bills. We took pride in trying to get the best value for money for the customer so they are generic PSUs although we have talked about before how you know, just for an office bill, those are fine. I'm glad to see the chat is working now. Last night when I did script to watch the live chat messages were not showing and so I thought nobody was chatting but then man, I actually went over to the chat function of ReStream, and dami palang dami palang nag chat chat but although may mali talagi ReStream, it wasn't showing up on the live. Sub guys, sub liya magnaye. Wow, it's like all the best hits, all the regulars in the house. Yeah, so it is not actually a 4090 although we do have siya kulang parang contest. So we have the 4060 from ASUS. Thank you to ASUS, they sent us this card for review. Is that the card now that we have? No, no. Sorry, just two. This does not look like a 4060. Is it the 4060? No, we have an ASUS na parang, we are a fan. To the one who asked kung pogiba siya, ito sagot mo. There you go. Apparently a lot of people ask us this already that we have a preset answer on hand. This is, no, no. It's not the 4060. The sign of the card is different. And I think the 4060 is still in here. Yeah, it's heavy enough. Thank you to ASUS. This is a custom order, 4070 MSI Gaming Extrio. And this is also a custom order, colorful 4080. So, actually I am a bit sad now that you mentioned that we don't have a 4090 to display. Although the ones that we have routinely on stock aren't even the 40 series, except for the 4070 over there. And we do have a 4060 actually, na single fan from Zotac over here. Oh no, this is a different 4070. Apparently. It's not twin edge, so it's double fan but why is it so small? Okay, got sidetracked here. Oh, this is reserved for a build I think. You should pan away a little so that you don't see the name of the customer. We should have a 4060 single fan here. But I don't know where it is. Na benta na ba yun? I'm going to need to check. Although usually I check every Monday kung ano ba na benta for the week so that I can restock. Alright, so I will do the you can see the pal over there. Let's talk about the pal a little bit. Sa pesto it's just a 4080. Malayo nang magtragin performance of 4090 sa 4080. Although to be fair to the customer who cost them or did that, they wanted the other 4080 of colorful, yung neptune, yung mas mahal but unfortunately they were sold out. So up level 8, Palbasar. Thank you for being a YouTube member as many of the people in chat are. Thanks. So the income is really appreciated by the shop. I mentioned many times that the shop burns money because we have to supply our chief source of what we sell our hardware and it's expensive to buy all of this hardware to keep it on stock. So things that give us pure income pure income in the sense na we don't need to put money out but we really appreciate. So those are the YouTube members the ads from YouTube. Of course our sponsor CD-Key I would point to something but we don't have any CD-Key para Fernalia. The merch and also the cleanings that's why we're super big on cleanings GPU cleanings, rig cleaning because that's basically pure income. We have very little input that we need to do. So yes, actually this is a nice mousa. This is my brother that's reviewing it. I'm trying to figure out mice now because I recently shifted to a steel series na wireless and I'm not really a big fan. It's like it's like na kukulangan ako. It's lacking the oomph that I'm used to for my usual Logitech G602 I think. When we need another sponsored video Kaiser we will do a RAM download tutorial. I'm gonna just sell my soul and recommend to people that that's the easier way to instead of buying physical RAM like this, that's expensive just download RAM. That's the way to go. We do actually Dreadnought. Thank you for commenting. We have just recently added laptop services. So we do laptop cleanings and basic laptop upgrades. So yep, that's one thing that we do now as well. Lea Magnaya basically answered it for me already. Our inventory is a lot less than other shops because we don't have a lot of capital. A lot of our capital I don't know why we don't have a lot of capital. I don't know why we're still in the red up to now to be honest. It's like we do a brisk business but we really have to pick the parts that we think will sell right away so we can't afford to although I'm guilty of this especially for cases but if I see a case that I like I'll stock it for the shop even though I don't really know kung mababenta ba siya kagad but we really try to pick the items na mababenta kagad. So that's why our inventory is quite limited compared to other shops. But we do make up for it because you can custom order from us even if we don't have the item on stock and this is a perfect segue thank you for bringing it up. Even if we don't have the item on stock just let us know what you need and we can usually get it from our suppliers same price. It's the same price with the same no BS warranty. Yung pagkaya ba lang from our things that are actually physically in store is that you might have to wait a couple of days for it to get to us from the supplier but like all of these aside from the 4060 card sent to us by ASIS review like this is a custom order this is a custom order mmm this case this is actually I would say it but he's in chat but this is actually your case. I bet you could probably figure out the guy who kindly we already had he was the initial custom order for this case we already had it and then another customer came in that also needed it as well and the first guy who had ordered it said well you know I don't really need it right now so you can order it again I'll just get the second one and you can give it to the customers already waiting so super thank you you know who you are this is your case so this was a custom order no this one really stuck what's this 5600 really stuck so those were not custom orders but we do have a lot of custom orders and I just want to show again we're very big on no BS warranty it's a one shop warranty and one year shop warranty and we don't make excuses na di ka namin papahirapan to claim on warranty no BS you bought it from us it's still within the oh take the light suddenly shut off you bought from us it's within the one year warranty we don't ask you for a lot of things usually even the receipt we have it already digital records you just give us your name and we can pull it up we don't need the box and things like that anyway this MOBO came in for RMA it was having stability issues I think the supplier hard hard reset the bios I mean they have a particular way hindi lang yung normal na update ng bios a normal consumer can do although we remember what a beautiful motherboard it's a white and grey scheme prime Z690 the customer actually already bought a new MOBO from us he couldn't wait but we don't know how long it will take actually already bought a different motherboard from us but this one he's going to be selling at sa second hand parts natin which is another unique feature of hardware sugar I'll talk about it in a little bit but we remembered that the USB ports I think were on the frits like they weren't working so we want to test those out before we tell the customer na hey we got your board back and everything seems to be okay because as far as we know parang they just updated the bios based on the notes that they sent us nothing physical was changed but yep and then speaking of the second hand parts we have them now here in front okay I'm just going to do a sweep alright wait let's do droid cams not on I've been talking I can see I am sorry um I don't know isn't it I had it on like it says it's on wait lang ha sorry sorry I know now what you mean it's not on but it's supposed ah there okay sorry when I pop out the chat it um it removes the droid cam the small picture within picture sorry so this is the case I was talking about not that you can see it basically I was just monologuing but you couldn't see what I was talking about this is the case I was talking about ah okay and here is the sorry the mobile is more important look at this beautiful motherboard although it had to be RMA love the love the gray love the little white finishes beautiful motherboard Asus has gotten taking a bit of heat lately for the x670e fiasco the hero boards although we have sold one of those um and you know we updated the bios as was instructed and it has not given the customer any problems in fact he's quite happy he's going for like 300 FPS in in war zone we also got a 4090 so he's one of our crazy ass customers that gali sa pera so wait lemme so I'll pop out droid cam and then it'll just be the small one alright so we're gonna do a sorry I'll just do a quick rundown through chat nah I guess we could run one nah I feel you ITX addict I am 100% my brother yesterday we're trying to sell one of our condos and we have a potential buyer but he still wants to negotiate for more and I was like no bento na natin and then he was like why do you need the money I'm like I always need money the shop burns money like crazy but I was like bitcoin is at a good price and I want extra money to shovel into bitcoin so basically I also just like burning money I love burning money for the shop I love burning money for my crypto I wouldn't say obsession but I am a little I have FOMO when I see that crypto prices are good I'm like oh maybe just a bit more toss in a bit more money into that pyre so yes I agree with you time to buy bitcoin 100% it's na tano subtech wolf cave I'm John Kenneth Asinto I'm sorry I didn't get to meet you but I did see your build in our sheet so all of our builds we have one google sheet that all of the staff can see and I see as well and I edit as well and whenever somebody gets a full build it gets put into that sheet and eventually it gets emailed to me so that it's easy to track the record so I did see your specs nice specs in the sheet I usually try to do although I have done it several times with customers in the shop na ilang ako kontin I'm kind of in my bubble here with my headset just talking to the camera can't interact with people also the customers that come in and also I feel a little weird I'm not used to shooting or streaming with a live with other people around here even to be honest when our staff helps me Jay before and the new guys and they help me with the cameras and I'm shooting here at the shop usually cameras on this side and I'm here on the left side na ilang pa rin ako so I'm not used to it so I try to shoot when when the shop is not during shop hours I actually try to time it on a holiday but there are no holidays in July so yun lang just going through the chat guys I heard I heard ITX addict we are down to make a video actually I keep pushing the guys for content everybody loves a good cleaning story a good cleaning video kahit shorts lang yun less than a minute na parang sobrang duminya and then like you snap your fingers it's gone or like yung I've seen a lot of videos online na day sa blower but although I don't really we use a blower but not that strong or like strategic implementation of the blower rather than just like I'm not really a big fan of the strong blower kasi the fans you don't plug the fans right they could spin in the wrong way and you could get dust in other places that you don't want them to be in so I am not a big fan of like a super strong blower no stop Lester we don't have any 500 watt PSUs I think we might be able to custom order sorry let's just make this the main first let me just go through chat and then we will go through like what's happening here and then like do a last tour of the place um sup Alistar Chua hello Mr. Demos09 yung kakilalo ko na nag-buy and sell ng 2nd PC build usually 10k below na Intel and conting rise in kasi kadalasan malang problema sa support pag Intel vs AMD um I mean so I'm not sure if the comment is implying that they prefer Intel builds because Intel is generally more reliable we we're brand agnostic we will give you what you want for Intel vs AMD although on the whole reliability issue wala mong problema yung neither brand has reliability issues and actually the CPU rarest components to have a problem with I mean any component any piece of hardware can break down, can cause you problems but a lot of the time it's not the CPU it's very rare that we've actually had to send back a CPU to the supplier regardless of brand, regardless of AMD or Intel so if it's just like in terms of the CPU breaking down, both are very very reliable in terms of stability if you're saying that AMD is rising less table and windows compared to Intel I wouldn't say so, we've done a lot of rising builds, especially the 3000 series AMD really hit its stride 3000 series so good, good value for money 5000 series, also excellent value for money especially now, 7000 hard to recommend kasi medyo mahal pa rin siya you need a DDR5 motherboard you need DDR5 RAM and DDR5 RAM itself I'll get to it in a little bit has been a bit problematic for us but rising 3000, 5000 sweet man, no complaints same rin naman with Intel, we've carried not so much to 11th we kind of stayed away from Intel for a little bit kasi price to performance they really weren't a good marker or peg against AMD but with the Intel 12th gen and 13th gen, very competitive again so we've been talking those as well I want I want liya magnaye I want, unfortunately are none of our we have a primary supplier for fractal design but they don't carry it and there's another supplier that supposedly carries fractal design in the country and I reached out to them like a month ago say that hey, take my money I want to buy stuff from you but they never got back to me and they still haven't gotten back to me so I guess I'll try again next week but that's where we are with fractal design where generally our supplier is good here we have a let me just show off the torrent off the one side here ta-dun so we have torrent we have another FD, we have a bunch of fractal design cases so you can check the side we've sold a lot of them we recommend them highly to our customers and we would love to get the north and the terra but our supplier doesn't have it which is kind of annoying sub, kemovehas we do not but we can get it as a custom order kebeni we do not also have update although generally prices have been dropping thank you for that keizer oh I guess I should go back to the other feed is there a problem running quad channel ddr5 especially enabling xmp I'm going to be honest we haven't had a lot of quad channel or four sticks of ddr5 mostly it's just been two sticks so I really couldn't comment from personal experience what I can't say is that even with two sticks it has been problematic for us getting xmp or the amd version of that basically overclocking the ram it has not been as smooth sailing as ddr4 and I attribute that to well it's new tech and as always with new tech there are birthing pains and it's been kind of annoying to be honest kawawa na customer he bought ddr5 ram from us five months ago six months ago maybe they broke down on him and then they broke down on him again so the second time that they broke down on him we said okay dina namin pipilitin itong ram na to so even if we had it on stock we wouldn't replace it we gave him full credit and he bought a different ram ram brand from us so may birthing issues in ddr5 personally I couldn't say we haven't had a lot of experience if going for sticks adds to the instability but even with two sticks medyo alangan na kami medyo daisi na siya this is on both platforms amd and intel so a bit of it's not as rock solid yet as ddr4 usually xmp is no problem but ddr5 has been problematic subtech will cave shout out to the second hand nhd15 being sold now in the second hand parts list stop alasatruwa I actually recently bought a laptop for myself because I've been spending a lot of time away from the shop and away from my keyboard because I bring my kid to school now and basically I wait for her to finish school so I basically have two hours where I need to be productive but it's very hard to be productive with just the cell phone so I bought myself a laptop it's not a bonkers laptop it's kind of like a mid range laptop although I got it with a good screen I'll be reviewing it also for the channel I'm super happy with it actually so it's an asus s14 flip OLED it's unfair to try to edit videos on it because that's not what it's meant to do it has an iGPU AMD I think it's a 5500H or something I'm not too familiar nothing breaks nothing breaks like the stream suddenly cuts out or anything but I'm super happy with it because for what I needed to do I'm going to mention it in the review for laptops you really need to be clear with yourself para you're not let down but just to see if it could edit audio no problem so actually the last podcast that we put out last Friday I edited that Thursday on the laptop audio no problem although that's kind of like saying if your system whether it's desktop or a laptop has problems with a basic audio we do have a couple of customers that are musicians and they have 92 channels or some crazy ass number there you do need like a high end CPU for that but just like a monologue basically which is my podcast you should be able to handle audio but it handles audio beautifully and I don't even use a low key program I mean a low resource program I still use DaVinci Resolve because I found it easiest to edit even audio in DaVinci Resolve and since I had time I was like okay I'm going to edit price watch also which will come out on Monday and so it can even but it's not enjoyable experience and it's only like super basic, cut cut cut but this is 2k video it was kind of like a long video so you have to load that into DaVinci Resolve and then you're working with that you're cutting it and things like that so I'm super happy with the S14 Flip OLED and yes it is OLED because I can't go back no more because I got spoiled when Asus sent over the one of their OLED monitor's review I didn't say the designation and I ended up buying one for myself so I can't go back but I'm super happy with the S14 Flip OLED and we can do laptop content now since I do have one now and yeah let's do that Undervolt using Total Stop I'm going to have to research what that is I mean Total Stop is that the usual for undervolting? I don't undervolt because I have my desktop the updated price is I think it's $1,750 for rig cleaning and cable management but we knock it down or is it $1,250 but sobrang laki nung discount nya if you're an old hardware sugar customer it's like $9,50 na lang for hardware sugar customer then $2,250 for GPU cleaning that it becomes $1,250 atta I think it becomes $1,250 atta for the GPU cleaning I answer inquiries like this all the time but the problem is I just copy and paste for my notion and na kaseyib na rin sa stream deck ko so the numbers really don't make an impression here anymore I just click a button and lemala ba siya numbers when I need to reply that but for the rig cleaning we do change the thermal paste to the CPU and of course for the GPU we also replace so yeah that's included for sure okay further actually we just restocked the 6600XT somebody had already bought the previous one and this is just a new three stock which came in yesterday actually so we do have that alas sir we actually have the 4070 and 4060 on hand the 4080, 4090 is na medyo mahal so we usually do that as custom orders and again we do have a custom order we failed the custom order 4080, here is that beauty over there 4080 but if you need 4090, 4080 best will handle it as custom order you also get a lot more variety like you can let us know anong brand, gusto mo and things like that even the 40 series kind of limited what we have we just have the Zotac 4070 and even our 4060 is Zotac but I don't know where it is usually the GPUs are just right behind where I have the camera now over there but I don't know it's hard to tell from that little itzy bitzy picture but I don't know where the R4060 is nakalimutan ko na yan I mean I'll have to check if where it went oh no sorry I hit the back button I was like leaf side? no no not leaf side is the 700X3D stronger than the 5800X3D in terms of performance it is DDR5 just in terms of raw numbers for sure mas malakas yung 7900X3D I think the question is more like is it better value for money probably not when you go up to the highest bunkers level it's just diminishing returns better value for money 5800X3D although it's really kind of apples and oranges because AM4 yung isa so it's unlikely that you're in a scenario where you have an AM4 system and you're thinking well if I want to upgrade I just need to upgrade the CPU but if you're building a new system malamang you want to jump to AM5 already just because it gives you more options later on down the road if you want to upgrade because it is DDR5 already it is the AM5 platform which will be supported by AMD in the succeeding generations but just in terms of raw numbers for sure the 7800X3D would beat the 5800X3D but better value for money that's a different story stop Kim okay lang naman JP no worries we still have space pa naman actually some of our staff have have suggested that we charge a storage fee for items and for builds and what not but I don't really want to do that because I'm not big on penny counting oh 14 days lang yung maximum namin every day may 10 pesos of fee I'm not a nickel and dime kind of guy we can nickel and dime you and we can probably earn a lot of money from that because it's just 10 pesos from one person but it has multiple items and there are multiple people that's a nice steady stream of income but again I don't want to run a shop I mean it's kind of like a bank I hate banks na they charge you money if you withdraw not from your home branch so let's say my home branch is BPI Salcedo and then I go to BPI Lagaspi and I'm like oh I need to withdraw money and they're like oh sir may charge pa ron ganyan although hindi yung BPI in fairness nagsimula na ito yung BDO grave yung BDO may nickel and dime kadag galaw mo sa BDO you need a statement of account yung list of transactions oh yan may buy it yan per page pa ron ganyan so I hate it as a consumer when companies do that to me so when it comes time to business time I also don't want to be that business that nickels and dimes the customer so we're not going to do that hindi masamaloob namin if you leave it with us for a long time in fact that's a badge of trust that you trust is enough at your expensive hardware I don't know if you're listening I won't say your name but he also listens a lot to the live streams or if you're listening to this on the VOD but we had a customer he bought like the pira kahaya na bill $40.98 about two of them it was with us for a month and I was joking with him because he has bought from us before that I have him on Viber I'm joking with him na if you leave it in the shop for too long the guys are going to benchmark you know what they mean they can use it I'm like no no they're going to get spoiled so we have some bonkers builds and components that are with us for quite a long time and we don't mind it's a badge of trust and I think you should be able to leave your paid for hardware well that's also why we ask for payment upfront may hirap na if you say you're going to buy it and then we'll serve it for you and then you haven't paid for it yet because we need the capital namin yung pera na yun so I think it's a very fair exchange now we ask you to pay for it upfront but once you have paid for it leave it with us man whatever long as you need it that's really no problem with us so I mean we could have a nice little revenue stream from that but I don't want to nickel and dine people I hate it when companies do that to me as a consumer banks ATM withdrawal fees kalokuhan 10 pesos for withdrawal buti nga nags withdraw ko from the ATM rather than clogging up the queue at the bank so irritating yung mga software ngayon puro subscription basis na hate it it's not the way I want to go probably what is hardware sugar doesn't make any money but at least I can hold my head up high and say that we're running the business I want to run except for the whole burning money part normal laptop normal laptop Asus S14 Flip OLED but I'm very happy with it to be honest I'll gush about it more in the review you can tell that Raphael James has been a YouTube member for a very long time is reaching 2 years to be honest we do need to update that Raphael James I will ask Ren to fix designer to come up with something to be honest when we did that we had no idea inkling at all that we would have any members reaching that length of time na members we do have a couple that have been super with us for that length of time so we should really come up with badges if you have any suggestions email us post it to our messages or something although I do like the number because it shows how long you've been a member on the channel the problem is the badges are so small so it's hard to get really creative with them so I mean you can go abstract with an icon but then that doesn't tell you like how long you've been a member and you would know but other people like if you comment on the stream other people wouldn't know oh but iba yung icon ito but it wouldn't know like sobrang tagal mo na so I kind of like the numbers but I'll have I'll discuss it with Ren the ROG PG number number uq sorry these are all from north bayu and I really recommend north bayu so we have a bunch of monitor mounts wait full me once, shame on me I won't make the same mistake again so north bayu I think this is the f1 model I also used the same we actually have one here oh we have three now in the shop the second and then we have the third over there anyway particular I used one as well the monitor just hanging off here to the side so less clutter and then over there on the right less clutter as well and then we have ross's monitor here which uses to answer chat which is super important except when he makes me kulit ross if you're watching you know what I mean no no I didn't say the thing it was just like because I answered it so I was like only half yes sir we do in fact if you go to our youtube channel and then search live drive sometimes I personally deliver them I've driven to Laon I've driven to Bulakan Bulakan is quite near actually Batanga, Subic, so up north, down south I actually enjoy doing the I enjoy doing the deliveries outside of Metro Manila because I like driving so I like stretching the legs of my car if it's not something that's feasible for us to personally deliver yes we ship nationwide we've shipped as far as Mindanao harder to reach parts of Mindanao we've sent full bills too so yeah we ship nationwide so yes our resounding is actually that's not chocolate dito sa I spoke in like a true crypto enthusiast alright alright so I'm just gonna I'm quite hungry like I had a very light lunch and I had to deliver so actually I'm gonna have a big announcement yeah so just to report that Rafael my brother will not be as active for the shop and the channel anymore for the next year because he has gone abroad to study yes so he is a lawyer like myself, like my father and like my grandfather before us, before him so yeah so the last video you saw from him about the chair he'll try to do content in the states and I imagine that would take up some of his time I am not an LLL myself my dad is so I imagine that he would be he'll be a little busy because I'm gonna mention I'm quite hungry now so I gotta wrap up the stream like with all the other things I wanna talk about but I'm quite hungry because I had a light early lunch because I had to go take my brother to the airport today because his flight actually leaves around now around 6 o'clock I think because he's going to be in the states so he will try to do content over there but it won't be as regular as it was before so yes so just bit of an explanation of why I'm hungry and why I have to wrap up the stream although I'm having a good time I'm gonna I'm gonna send this to him guys for reals man for reals I think you've earned it Lea Magnaia next build will be personally delivered but you're just in Metro Manila I mean it's not that far actually my wife pointed out that when Rafael gets his LLM I will be the least educated member of my family just a quick flex for my family members my dad is a lawyer and also an LLM from Georgetown my mom is an MBA my sister is a doctor practicing in the states and her specialty is psychiatry after the basic doctor-doctor thing she went on to a very select subspecialty she's a child psychiatrist and my brother now will have an LLM which makes me my standard JD as the least educated member of my family which I find quite wild to think about actually to be honest so these are the second hand parts we've moved them out of the storage room which was over there now over here just to entice people to come through the door maybe you want to buy some second hand parts and here's the NHD15 mentioned already in chat if you're looking for a big boy cooler nobody is as big and as coolingness much as the Noctua NHD15 I've talked about this before actually this is brand new but it's being sold second hand parts monitor not too good for gaming not too good for creators or people who need to edit and stuff like color correction things like that so the next part I want to talk about is this one, I'm going to bring this home this was in the shop for a super long time our lightsaber stick was in the shop for a long time but I'm going to bring it home because I'm trying to come up with a small studio in the shop the lighting has just really like I can't figure out lighting I'm not a cinematographer I don't have any experience with this lighting camera angles and stuff like that so I get it's really a lot of trial and error for me so I've been up and trying to come up with a small home studio so that I can shoot a role comfortably in and it will look good but so far the quest continues as it works alright so here are this whole pile is the office computer build I spoke about it's a generic fortress case generic PSU for office builds okay naman e I know the old droke the bomb has been planted but for office builds generally alright and to be honest the office builds we've done with these generic things we don't have them often they haven't come back actually so what I do want to point out what we're proud of of this pile is that it's for an office build but we didn't cheap out on the part so the motherboard is ASUS the RAM is G skill do I have the RAM here no it's just power cables here the monitors are viewsonic and then the gpu was a 730 which is a terrible card I wouldn't I would not recommend this card at all to anybody so why the hell did we buy it for this build because the customer requested for it and that's a lot of that's one consideration that a lot of people online who comment don't consider like why the hell would you stick that into a build because the customer asked for it maybe they have a requirement that requires the 730 it's not gaming for sure I mean it's a terrible card I wouldn't recommend it myself but we have a whole bunch of them now to go into the office build they specifically requested for the 730 but in spite of it being an office build we really tried to give them value for money so 10th gen intel ASUS MOBO ASUS GPU G skill RAM Logitech I know those came in already Logitech, mouse and keyboard set up more MOBOs the the Logitech keyboard and mice do well thing it's like a combo thing where are they though I hope they're in here somewhere in this whole pile because I'm pretty sure I ordered them already actually these big orders really stress me out to be honest if you make a mistake that's a big mistake for the that's a big mistake for the PC shop to swallow and to be perfectly candid, I make mistakes all the time as the guys in the shop who Cooper is I'm the one who primarily interacts with the suppliers sometimes to be varied to suppliers to make the mistake like I specifically requested for something and then they got a different variant sometimes I make the mistake I don't usually ask them to take it back anymore we just sell it in the shop but you know he have 35 pieces of the GT703 that is not gonna sell well in the shop okay that is slap me in the face I am dumb swear word kind of like mistake these kind of things really stress me out because we have to be super sure that we get all the specs right and we have the money involved and things like that I much prefer just like a short story about this customer who got this guy so usually ko usup ko yung daughter around my age actually they know each other but Rafael already forgot I don't know but I heard dad so he was a senior citizen took over following up and things like that I have difficulty explaining to the older gentleman like PC stuff so he's like okay so have the parts come in okay can you finish partial delivery of like 10 no no we're still waiting for the CPU and if the CPUs haven't come in then there's no way that I can finish the build I mean it's not like I can cobble together a build even if it's missing a CPU so I mean like little things like that but this hopefully it will be gone by the end of next week because CPU na lang hiniyintayin and actually I got word already that's it's ready I'll have our guy pick it up on Monday and then we'll have the joyful task and we can have a build party of putting together all of these office computers yeah and there's still some haggling involved about the price I was a little peeved to be honest just like a little peeved because he was like can we get this done and I'm like yes we're still waiting for basically it was like I didn't like the story it was like a money thing basically I pointed out to him that we were doing him a service because we were giving him credit essentially so just cleaning up the LS720 CPU block bit of dust no credit we're paying for it but you haven't paid for the entire thing we're fronting some money for this and that's credit we usually don't give credit so this is also why I get in trouble with people online I get frustrated sometimes with customers I'm trying to point out that we're doing you a solid man we're doing you a favor we don't usually give credit in this case and he's like you're not giving credit and I'm like what do you call buying parts even if you have we will order the parts even though you haven't fully paid for it yet I mean that's credit right but anyway we don't expect any drama from these builds the CPUs will come in and then hopefully we will have them out by the end of the week and then this space will be free again so ko sayo eh that's what I like if the name of Bitoy Kongko should be familiar also to people who frequent the stream because he is a frequent commenter and then just to shout out to the old boys Bitoy is actually one of my longest stalkers because he was my he was my seatmate in high school but our school ID numbers were right beside each other and when I went to check my college they have a long list of people who passed the entrance test my name and then Bitoy's name were right beside each other so he is my longest stalker since high school and I actually find it funny that when my high school classmates were very close as a section which is nice and as you get older you appreciate long standing friendships na you haven't seen each other for a long time and I haven't seen Bitoy in a long time but when you see each other it's like para nagsembrake lang kayo it's been like 2 years since pandemic or something that we haven't seen each other but when you see each other para nagsembrake lang kayo it's like you're up to your stupid you're up to your stupid stories and stupid shit again that's the beauty of long term friends but I always find it funny my high school classmates comment on the streams because they have no fear of privacy it's like their whole name it's not just Bitoy, I've had other good friends also comment in the streams it's their whole name and I'm like do you really want your whole name out there and I think for us, for my generation privacy isn't really that big of a concern that's why I find this whole obsession with privacy you have to report it to the national privacy commission and whatever do you think Facebook cares about the national privacy commission and data privacy and data confidential, bababla, privilege and whatnot and there are laws about it and things like that but I think on a very practical level it's a lot of people of a certain generation prefer the usefulness of companies having certain information all of your information but we don't get as bent out of shape about it and it's not even me even older folk, I listen to the podcast of Malcolm Gladwell religiously and he was saying there are a lot of scientific experiments now that we can't do, that they did in the past because there are a lot of privacy issues now but wouldn't you want that scientific data to be available because look how helpful it's been for medical research and he's like Facebook has hoovered up our data ages ago, Apple the different IDs that they've tagged to our digital personas and it's kind of like the cat has been out of the bag so again it's not just me and I know it might be an unpopular opinion but I'm very I'm not as like a privacy watchdog as a lot of other people and then we had one we had one guy we were running a giveaway we had one guy who PM does we were running a giveaway so the normal way we run a giveaway is that you need to submit certain information via Google Sheet like your name your email address and your contact information so that if you win, if you're randomly chosen we know how to contact you, we know how to give you your item that you want and then we had one guy PM us over Discord and he was like what you're doing is illegal you cannot ask for the personal information of people who are joining contests like that and again I don't whack it in people's faces I mean you know some people that once they become a lawyer it's not enough to be called Anton it's not just enough to be called here on the screen like your Facebook name has to be Anton and you know that it's just really super role eyes for me ok I find people who do that obnoxious I mean I think that it's a fair word or the meaning of obnoxious so I don't go parading it like that but if you have somebody who is completely wrong about the law telling you that I am wrong about the law it's like not really you know we are not wrong about that so I mean it's a damig fang sinasabit and actually yun yung malungkot the whole point of why they are lost is that people can look them up and they should be understandable even to non-lawyers so that we all know what we are not allowed to do so this guy was quoting the law but he had no zero understanding in fact the NPC came out with a bulletin or something saying cautioning people you want to be cautious about where you give your information what contest you join implicitly saying na pwede yun hindi baol yung pag-ingin ng information na yun we are telling you to be careful who you give it to obviously it's not a prohibited activity we are telling you to be careful who you give it to and then you know and I what you got me further about it was na parang he was saying na parang yun dating ba as a favor to the shop I'm flagging your attention I'm pointing this out to you because you guys are in the wrong and I just want you to follow the law but zero 180 degree the law says the law is black and white the law says it's black and you guys are saying it's white completely like nowhere in the law does it say that it's prohibited so you get all types and yun for him privacy was a super big issue so much so that he misread the law I don't know kung law student yun I hope he if he is a law student he need to harder before you take the bar yun but I just find it funny when my friends pop online and they comment again for our generation it's not super it's not super big he didn't you should have seen he messaged on discord and he also messaged the google form that you guys are violating the law exactly thank you plus it's not against the law that too we joke about it but it's eerily true recently I need new shoes so I've been buying different sneakers looking at it online and then facebook would pop up with sneakers ads did you I thought it was a PM I didn't know it was public I thought it was a PM but I think Rocky the programmer I replied to him in a nice way alright so I'm sorry guys I would love I didn't actually I think I took a screenshot but I haven't posted that yet to screen scratchers ha did di it's like yun yun nakakalungkut ay unlayo talaga dun sa batas nowhere ay rin yun nga the npc had a circular about it saying that you need to be cautious that means the activity is allowed if it weren't allowed then the npc wouldn't have to say be cautious because then it would just say you can't do that because it's illegal naayut talaga kabe and to be honest nga that's what irritates me about crusaders they're like oh they're on their high horse but then they get the basic facts they can't even get the basic facts or the basic lore right kakainis this is basically what live in the shop live from the shop boils down to but I gotta go home because I gotta go eat because I'm super hungry the food on the plane because shempre Rafael my brother does not go he enjoys the flight if um let's just put it like that so thank you for joining me for another live from the shop tomorrow is a game stream so the last of the month will still be doing starter troopers and then we will release the NAS video which has been online which has been up for members since this morning I believe if I remember correctly GG guys stay safe have a good weekend please do tune in for watch the podcast which released yesterday tune in tomorrow for the live stream I don't know what time it really depends on when my kids will let me play and then the NAS stream we can sorry I'll just feature the NAS very briefly so tera master was super kind enough to send this over for review and I mean super kind because I was just expecting a 2-bay NAS but it's a 4-bay NAS and it's really one of their top NAS units the F4-423 rocket a programmer took it for a spin and I think maybe the most telling part is that we're using it we're relying on it now and I think a lot of our storage will be put there for online just we're just waiting for PLDT to give us a static IP address so we can do the cloud thingy I've always wanted to do one but we don't have any on stock we've done several for customers but we don't have a go to DIY NAS actually you need very basic specs so you don't need even the CPUs like we have a 10 13100 you don't need that you can use your old computer so even for like a DIY NAS medyo op ang mga stock natin alright so stay safe, have a good weekend thanks so much for watching guys see ya, I'm so hungry


Hey, Psych2goers. Welcome back to another video. Before we start, we would like to thank you for all the support you've given us. Psych2go's mission is to make psychology and mental health more accessible to everyone. Now, back to the video. Has anyone ever ghosted you? For one moment, you were chatting with each other all the time, and the next, it seems like they've disappeared out of thin air. Ghosting is a term used to describe someone who ends a relationship by suddenly withdrawing from all communication. It can be extremely painful for someone who has been ghosted, since they are left with no explanation at all as to why the relationship didn't work out. So, here are eight signs someone is going to ghost you. Number one, their texts are brief. Has their text responses become shorter and shorter? Maybe you two had been texting non-stop before, where it seemed like you could never run out of things to say, but now you find their texts to be too short. Instead of continuing the conversation, they only reply with one word answers. If this happens frequently, it may mean that they're losing interest and are preparing to ghost you. Number two, just emoji responses. Have they started to respond to you by using only emojis? While there's nothing wrong with using emojis when texting, responding with only emojis all the time, especially when you've written something thoughtful to reach out to them, may be a cause for concern. Using an emoji is an easy response when you don't really care or know what to say, so their use of only that may be a sign that they have lost interest in you. Number three, you always reach out. Are you always the one to start the conversation? Although you may have texted a lot in the beginning, now they only write to you if you message them first. This habit of not reaching out may be a sign that they are no longer interested and are looking for a way to end things. Note that sometimes they may just be genuinely busy, so it's important to communicate your concerns to them first so that they are aware of how their actions are affecting you. Number four, poor grammar and spelling. Has their texting style changed all of a sudden?  This sudden change in how they text you may be due to a lack of effort and a loss of interest on their part. Instead of spending time to express what they mean thoughtfully as they had in the past, they may turn to use shorthand and slang to send a response. This may be because they are no longer invested in the relationship anymore. Number five, they're always busy and cancel plans. Have they suddenly become busy all the time? Do they postpone your dates often? If you once hung out or went on dates often and now those meetings have come to an unexpected halt, then they may be trying to ghost you. While it can be tricky in the beginning to figure out each other's schedules since they may be genuinely busy or shy to meet, this unexpected tendency to delay and pause your dates may be a reason for concern. Number six, something doesn't feel right. Does something about them just doesn't feel right with you? At the end of the day, going by your instincts may be the best. Maybe your partner has been acting differently lately or their tone and their text sounds off. Even if you may not be able to consciously point to what is wrong, your gut feeling is usually right and so you may want to address or speak up about your worries and concerns to them first. Number seven, you haven't been on an actual date. Do they always postpone going on a formal date with you in favor of just hanging out to watch TV? If they've promised you to go on a date at some restaurant but keep delaying it in favor of just binging TV all evening, then it may be a sign that they may not be as invested in the relationship as you think. While there is nothing wrong with staying at home, this tendency to push off going out together in a formal manner when you've asked to may be a sign of their lack of commitment and investment, which may lead to them ghosting you in the near future. Number eight, they aren't introducing you to friends or family. Have they told their friends or family about you? Not everyone introduces their partner early on in a relationship. However, if they keep you a secret from everyone they know, it may be that they are not interested in a committed relationship. If you aren't mentioned to their friends or family, even in the slightest, when you're well into your relationship, their lack of commitment and placement in their personal life may mean that they plan to ghost you in the future. Do you relate to any of the signs mentioned here? Has anyone ever ghosted you or have you ever ghosted someone? Tell us about it in the comments below. If you enjoyed this video, please like and share with others who may find it helpful too. Subscribe to Psych2Go and hit the notification bell for more content. All the references used are also added in the description box below. Thank you for watching and we'll see you in our next video.


myself, Sanjay Udge, assistant professor, department of electronics engineering, Valchand Institute of Technology, Solapur. Today, we are going to discuss DC circuits, delta 2 star conversion. Learning outcome. At the end of this session, students will be able to analyze complicated electrical circuits. Guidelines. Introduction, delta network, star network, delta 2 star conversion are references. Introduction. Review of DC circuits. In the last videos, we discussed basic concepts of DC circuits. It was included Ohm's law, Kirchhoff's current law, Kirchhoff's voltage law, so as to analyze and simplify the given DC circuit. What is the necessity of delta star network? To simplify the DC circuits, comprising of series and parallel resistances, along with the voltage sources, DC voltage sources. We used to apply KCL, KVL in order to find out the currents flowing through a particular branch or voltage across a particular resistance or a total current of the given network or equivalent resistance of a given network. So, there are some networks in which we cannot use Ohm's law and various theorems. In that case, we have to convert the given network successfully between delta 2 star and star 2 delta. The successive conversion between delta 2 star and star 2 delta will simplify the given network. And finally, you can get the equivalent resistance value, then the total current or the voltage drop across various branches current flowing through the various branches. Delta network. This is a delta network consists comprising of three resistances R1, R2, R3 connected between the nodes A, B and C. This is a star network. If you observe one end of R A, R B, R C, they are connected to a common point called as O, whereas other points are going to the different part of the network. So, this is a star network. Conversion from delta 2 star, node 1, this is 2, this is 3, this is a delta network and this one is a star network 1, 2 and 3. Resistance between delta resistance between 1 and 2, it is R1, 2, this is R2, 3 and this is R3, 1. This is R1, R2 and R3. These two electrical circuits are said to be equivalent when the voltage resistance between the two terminals is equivalent. Now, let us find out the condition. First, terminals between 1 and 2 for star network, it is R1 plus R2. It is R1 plus R2. And for delta network, it is R1, 2. R1, 2 is coming in parallel with the series combination of R3, 1 and R2, 3. So, I will write it as R1, 2 parallel R2, 3 plus R3, 1. I will write this equation as R1 plus R2 is equals to R1, 2, R2, 3 plus R1, 2, R3, 1 divided by R1, 2 plus R2, 3 plus R3, 1. That is this equation number 1. Similarly, between terminals 2 and 3, between terminals 2 and 3, it is R2 plus R3, 2 and 3, R2 plus R3. Coming to a delta side, between 2 and 3, this R2, 3 will come in parallel with this. These two are R3, 1 plus R2, 3. I will write it as directly R2, 3 parallel R3, 1 plus R1, 2. I will rewrite this equation as R2 plus R3 is equals to R2, 3, R3, 1 plus R2, 3, R1, 2. Let this be a equation number 2. Now, coming between terminals 1 and 3, for star, it will be R3 plus R1. And for delta, it is R3, 1 parallel R1, 2 plus R2, 3. I will rewrite this equation R3 plus R1 is equals to R1, 2, R3, 1 plus R2, 3, R3, 1. Let this be a equation number 3. Now, I will subtract equation 2 from equation 1. What I will get?  Let this be a equation number 4. If I add equation number 3 and 4, we got R3, R3 will get cancelled. Then R2, 3, R3, 1 will get cancelled. It will become 2 times R1 is equals to 2 times R1, 2, R3, 1 upon R1, 2 plus R2, 3 plus R3, 1. 2, 2 will get cancelled. So, R1 will be equals to R1, 2, R3, 1 upon R1, 2, R2, 3, R3, 1. For converting delta, R1, 2, R2, 3, R3, 1 to get this R1, R2, and R3. It is very simple. As we derived one formula for R1, R1 is equal to product of these two resistances divided by summation of this. Similarly, how will I write R2? I will write R2 is equals to R2 is coming over here. This is 2, 1, and 3. R2 is connecting to known junction number 2. To this junction, which delta resistances are connecting, this is R1, 2, and R2, 3. So, to find out R2, it will be product of these two delta resistances, which are connecting to same junction 2. So, it will be R1, 2, R2, 3 plus summation of delta resistances. Similarly, what about R3? R3, you can write, R3 is going to junction number 3, and same junction number 3, which delta resistances are connected, this R2, 3, and R3, 1. So, R3, I can write it as R2, 3, R3, 1 divided by summation of R delta. So, it is very easy, no need to remember the formula. Let us solve one example. Two deltas are shown whose values are 10, 10, 10, 10, 10, 10. This 1, 2, 3. It is required to find out the resistance between 1 and 2. So, what I will do? First of all, I will solve these parallel networks. So, by solving these parallel networks, I will get this as 1, 2, and 3. 10 and 10, it will be a 5, 10 and 10, 5, and this will be a 5. Now, resistance R1, 2 is equals to, it will be 5 ohm, and this 5 ohm, it will come in parallel with the series resistance of these two. So, it will be 5 plus 5. So, it will be 5 into 10 divided by 5 plus 10, it is a 3.66 ohms. So, this is how we can convert, we can get the value of resistances in this. Let us convert this delta network into a star equivalent network. So, this is having value 9, 9 and 9. I want this R1, R2, and R3, 1, 2, and 3. So, R1 will be equals to this R1, which is coming over here. It will be 9 into 9 divided by 9 plus 9 plus 9. 81 divided by 27, it is a 3 ohm. Same is the case for R2, which is coming over here. R2 will be equals to 9 into 9. R2 is coming over. So, it will be a product of 9 into 9 divided by 9 plus 9 plus 9. 27 comes out to be 3 ohm. R3, which is supposed to be coming over here, it will be again 9 into 9 divided by 9 plus 9 plus 9. It is a 27 again, 3 ohm. So, this is the exercise assignment. It is a delta network having values 3, 3, and 3 ohm. It is required to convert into a corresponding star network. To find out here, this is Rx, Ry, and Rz. Rx will be equals to 3 into 3 divided by 3 plus 3 plus 3. So, Rx will be equals to 3 into 3, that is 9 divided by 3 plus 3 plus 9. It is a 9. It is equals to 1. Ry, again it is same. To find out Ry, it will be 3 into 3 divided by 3 plus 3 plus 3. So, it is again 1 ohm. Rz also, 3 into 3 divided by 3 plus 3 plus 3 is equals to 1 ohm. Academic textbook, electrical technology, BL Theraja. Thank you.


Hi everyone, Joe for jaspyscasewrakes.com. Happy Saturday. We're coming at you with our last Filler box to knock out that 2012-2013 flawless. That's gonna be in a separate video. That should be pretty exciting We're gonna do the break itself first. We're making sweat it out a little bit We're gonna do the break itself first then we're gonna give away that flawless spot in a completely different dice roll at the towards the end of the break Big thanks and good luck to all of these wonderful people here There's all the teams in right there for the blaster break. Let's roll it Let's randomize it one and three four times for each list one two three and four So after four times we got Jay down to John four times for the teams one two three and four After four times we got the calves down to the Timberwolves To save my voice for the flawless break I'm not gonna read off all these names and teams but just for a filler break, but let's highlight some teams right here Brian Grizzlies Demetrios nicks for Demetri Brian. You got my Lakers Right some LeBron's could be pretty nice in there. Did I miss the I miss the pelicans? Blind oh no, Brian Frank has the pelicans in a blaster box. We'll see We'll see. All right. No trade windows in these filler breaks, too. Let's print and rip I did not were the autographs Yeah, we've been lucky enough to pull a number of the Herbert rookie cards I pulled a hollow one as well, but I think the autographs have been Turn on my camera have been a little elusive the angles here really quick Chris what's going on? Wow, he pulled the RPA and an auto out of 35. That's pretty awesome I hope he snapped pictures of that for for the social media team That was just that was just marinating in that case wasn't it? That's as good as it's gonna get Alright There's the final printout right here hot off the presses. Thanks everyone. I will We got more panini contenders Or Donner's optic football on the site We've got hybrid versions as well you know, maybe maybe we'll find some gold vinyls of the top rookies So we can knock out some of those tonight if you want to another case tonight We maybe have a few more cases after that so as soon as those fill you can post post another one I cut myself right here Wounded how did I do that? We got Wendell Carter, Jr. Piece of his jersey goes to Marco and the Bulls. Oh nice John Morant right there and a purple wave JJ Reddick That's that's Pelicans going to Brian Frank and Memphis. That's also Brian Frank with the genre That's not too bad. I have a little filler break Tybalt and Demarda Rosen right there All right, let's flip back to this list right here. We've got Jay down to Dimitri and everybody in between Let's get everybody into this new list right here This is the moment you've been waiting for the name on top gets a $3,500 flawless spot after five One happy person 29 unhappy people this sells out the flawless break Which will be in a separate video so one and a four five times good luck one two three Four and a one fifth and final time after five Good luck everybody Congrats to sorry to Diane right in that seconds Congrats to Jay goins. Congrats to you a $3,500 spot for a fraction of the price. Thanks for getting into this filler. We really appreciate it I'm Joe. I'll see you next time for that flawless break. Bye. Bye


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Hello, my name is Andrew Ocheski, and I'm a PhD candidate in the Griffin and Tret-Yukova labs. I wanted to talk to you all about some of the COVID-19 research we've been doing in the Griffin lab using our galaxy workflows, in a talk I call, a galaxy-based evaluation of optimal peptide targets for MS-based detection of SARS-CoV-2 in clinical. The COVID-19 pandemic has been one of the most significant and destructive global events in recent emery, with presently hundreds of millions of cases and several million patient deaths. A key component of controlling the pandemic is rapid screening for and diagnosis of the disease in patients. The two primary diagnostic tests are PCR amplification of viral RNA using patient samples and serological tests to determine the presence of antibodies. However, the multiple steps in QTPCR amplification come with the potential for introducing false positives. In addition, detecting antibodies for SARS-CoV-2 only verifies cases ex post facto. We believe that targeted mass spectrometry assays to detect specific SARS-CoV-2 peptides would aid in the global fight against COVID-19 due to the relatively robust nature of peptides and the speed at which such an assay could be performed. Targeted mass spectrometry assay for viral peptides would be non-invasive, fast, and highly accurate and would therefore be a valuable addition to the current testing paradigm. However, not all peptides are suitable targets for analysis. We hypothesized that we could use bioinformatics workflows in Galaxy to mitigate the need for testing peptide targets and determine the optimal SARS-CoV-2 target peptides a priori based on publicly available datasets, which constitutes the work I'll be discussing here today. This was performed using key workflows in Galaxy. In this first workflow, we use proteomics search engines to interrogate open source mass spectrometry datasets for the presence of SARS-CoV-2 peptides in order to create a panel that we could then use to search clinical datasets for specific high quality viral peptides. Through the use of in vitro cell culture datasets, clinical datasets, and other bioinformatics experiments, we were able to construct a panel of 639 viral peptides for use in downstream analysis. The 639 panel of peptides correspond to proteins throughout the SARS-CoV-2 proteome. Mathing peptides to the viral proteome yields matches to almost every protein in SARS-CoV-2 with several proteins having high sequence coverage. These tend to correspond to structural proteins such as the viral spike protein, the membrane protein, and the nucleocapsid protein. In the second workflow of our analysis, we leveraged our 639 peptide panel against clinical datasets using the PEP query search engine to determine which of these peptides are reliably found in actual patient samples. For this, we used seven sets of clinical data from COVID-19 patients harvested from the upper respiratory tract and deep within the lungs. The PEP query search engine matches spectra to a list of peptides of interest. In our case, our SARS-CoV-2 panel, and then determines whether the spectra might be a better match to peptides in a designated reference proteome. For our reference proteome, we used a combined FASTA file containing the human proteome, common mass spectrometry contaminants, and eight other coronaviruses to ensure specificity of these peptides for SARS-CoV-2. The results of the PEP query search were then filtered to retain the best matches to SARS-CoV-2 and fed into the open source analysis program's UNIPEPT and the NCBI BLASP to verify their specificity for SARS-CoV-2. After running the second workflow, we see that our initial set of 639 SARS-CoV-2 peptides is winnowed down to 87 peptides with a high specificity for the virus. Of these, most of the peptides belong to the structural proteins, again including the spike protein, the nucleocapsid protein, and membrane proteins which make up the viral particle itself, while replication proteins translated from the large ORF1 are largely lost. Second workflow also generates MS2 spectra that can be examined using the Laura Keats tool in the multi-omic visualization platform so that we could examine the quality of the spectra of peptides that pass through PEP query. Our rules for selecting good spectra are based on getting the highest quality matches possible. The product ions must be at least three times as intense as the noise signal, and there must be at least three consecutive ions in the B or Y ion series, or ions corresponding to the fragmentation of the peptide backbone. By way of example, there are two MS2 spectra belonging to PSMs observed in a COVID-19 patient dataset which passed PEP query validation. When we look at the spectrum on the left, not only are the product ions in and amongst the noise, but each series contains only a single product ion. We can therefore discard this spectrum, and the PSM is being real. By contrast, the spectrum on the right has a fairly complete consecutive series where the product ions are well above the noise. This PSM was therefore retained for consideration as a target. Ultimately, we determined that four peptides scored highly in PEP query of best manual evaluation and were present in the majority of clinical datasets. These peptides are presented here, where you can see the quality of their MS2 spectra. These peptides corresponded to mapping to the nucleic acid protein, which serves to package the viral RNA within the capsid. This highly abundant protein is also the gene most commonly amplified during QRT-PCR diagnosis of COVID-19, making MS detection of SARS-CoV-2 using these peptides as targets a complementary methodology to pre-existing assays. Our target selected, we wanted to ensure that these peptides would make suitably specific targets for SARS-CoV-2. In running them through UNIPEP, a metaproteomics application designed for taxonomic and functional analyses, we see that they all have some alignment to various strains of SARS-CoV, with each showing alignment to SARS-CoV-2, illustrated here by the increased font size. To see how specific these alignments were, we also used BLAST-T to align the peptide against the viruses. As you can see, these peptides show perfect alignment to the nucleic acid of SARS-CoV-2, more so than any other coronavirus, with the first peptide in the series being wholly unique to SARS-CoV-2. In addition, none of these peptides showed any alignment with the human host proteome. One issue we noted in our analysis was the difference between those samples collected from the upper respiratory tract and those collected from within the deeper lung. In looking at the unique peptides that ask PEP query validation in this group, we see that relatively few SARS-CoV-2 peptides are shared between these two samples, with few of our designated target peptides detected within the deep lung samples. Unlike the peptides detected in the upper respiratory tract, the PEP query validated viral peptides in the deep lung samples correspond to proteins throughout the viral proteome. Interestingly, during our manual annotation, we found that those peptides that pass manual validation have notably lower PEP query P values. In looking at those peptides which pass PEP query validation with a much lower P value threshold, we see that in upper respiratory tract samples, most of those peptides are retained, while the lion's share of those peptides which pass PEP query validation in the deep lung samples are then lost. In keeping with this observation, only a single peptide that will pass PEP query validation in the deep lung samples also passes manual evaluation, suggesting that these samples, which are inherently trying on the patient to harvest, make poor samples for mass picrometry-based detection of SARS-CoV-2. Include, four peptides from the nucleocapsid were determined to be particularly suited to targeted proteomics. They had high confidence matches in the PEP query workflow, were observed to have high quality spectra, and were observed in most of the clinical data sets that we analyzed. A manuscript detailing these discoveries has been written up and published in the Journal of Clinical Proteomics. For future directions in this work, we are working to determine the optimal sample preparation conditions for LC-MS analysis of SARS-CoV-2 patient data. In addition, we are working to investigate whether different strains of the virus can be differentiated effectively using LC-MS assays. We are working on this in collaboration with Waldemarie Carvalho and the Fleury Group and the Cervasta Lab in IIT, Bombay. I'd also like to talk a bit about a metaproteomics analysis we performed on COVID-19 positive samples to determine the degree of co-infection with SARS-CoV-2. Over the course of the pandemic, many patient samples have been collected and submitted to shotgun proteomics analysis as a part of the research efforts to combat COVID-19. While most of those approaches detect the infection by targeting SARS-CoV-2 proteins associated with the host response, we believe that the MS-based proteomic data sets can also be further utilized to identify and understudied what potentially important co-infection status of the infected individual. Bacterial co-infections during respiratory-related viral outbreaks have been shown to significantly contribute to fatalities, as demonstrated in both the 1918 and 2009 pandemics. Moreover, recent research reports have also shown that many fatalities attributed to COVID-19 viral infections could be due instead to patients' pre-disposition to co-infections. Diagnosing and managing co-infections can be complex, as it is possible that opportunistic co-infecting organisms are present in patients prior to viral infection or are acquired during hospitalization. For example, chronic bacterial infections associated with chronic obstructive pulmonary disease can be a risk factor for patients with severe COVID-19 infection. Additionally, some COVID-19 patients with severe presentation are subject to prolonged mechanical ventilation, augmenting their chances of developing nosocomial infections. Furthermore, the use of antibiotics to treat bacterial infections is especially high among COVID-19 patients under intensive care. The global overuse of antibiotics can lengthen the growing roster of antibiotic-resistant pathogens and present problems in pairing infective organisms with appropriate antibiotics in an effective and timely fashion when using culture-based testing for co-infecting microbes. Early diagnosis of multi-organism co-infection is also necessary to avoid complications during hospitalization. The work that we used in our proteomics analysis is based on two steps. Together, they're able to identify co-infecting microorganisms present during COVID-19 infection. In the first step, publicly available mass spectrometry datasets from COVID-19 positive and COVID-19 negative patients were analyzed in Galaxy using Compil 2.0 against a comprehensive database. The resulting peptides identified were then analyzed using the taxonomic search engine Unipep to identify species present in the samples. Those species with two or more unique peptides were used to create a clinically significant multiple-organism fast database, which the data were then searched against in Galaxy. Peptides matching microorganisms were validated computationally via PEP query and manually annotated and validated using the lower-key platform. Using these workflows, we were able to detect characteristics peptides from several microorganisms in COVID-19 patients. In COVID-19 positive patients streptococcus pneumoniae was detected, a bacteria known to cause pneumonia and other respiratory tract infections. Drugingly, we were also able to detect lactobacillus ramnosis, a probiotic, in addition to an unclassified pseudomonas strain. By contrast, COVID-19 patients also showed some characteristic microorganisms, namely pseudomonas monteliii, which is responsible for meningoencephalitis and actinobacteria, your signei, known for causing bacteriaemia. That included this study, meta-proteomic analyses of COVID-19 patients demonstrate co-infection with other pathogens. A letter detailing this workflow and our results was published in the Journal of Proteomic Research. We're continuing to develop and modify our list of co-infecting organisms by continued analysis of more samples of patients in India and Brazil. This information can be found at the URL at the bottom of this slide. I'd like to conclude by acknowledging all of those who work on this, on these studies in the Griffin lab, namely Dr. Pratik Jaktab, Subina Mehta and Dindui Anwen. I'd also like to thank our collaborators in the galaxy EU and then the Wollong lab at Scripps Institute. Thank you for your attention and I hope you enjoy the rest of the conference.