Today we're going to go over the work from home jobs tier list where I'm going to rank the best and the worst work from home jobs from S tier, which is the best to F tier, which is the worst. And I'm going to be covering a bunch of the most common work from home jobs. Most of these are going to be entry level, but I'll also include some mid level ones for people who already have a decent amount of work experience. And I've been studying this stuff for years and I've helped hundreds of people get these types of jobs. So I definitely know what I'm talking about and I have a lot of experience with the most common ones. So I know quite a bit about this subject and the first one on the list is going to be manual QA. And that stands for manual quality assurance. This is also referred to as a manual quality assurance analyst sometimes. But basically manual QA is kind of like what an editor is to a writer, right? So if a software developer was like a writer, manual QA would be like an editor. And basically you're just making sure that the software runs smoothly and doesn't have any glitches or bugs. And they're able to test products and find a bunch of these glitches so that your customers don't have to. And manual QA makes about $71,000 a year. Now you might be thinking you have to be a master coder in order to get into manual QA. And actually that's not true. You can actually get into this position without having a college degree or previous experience. But you do have to have the right skills and portfolio. And that's where careerist comes in and they offer online live courses to teach you the skills that you need to know. And according to coursereport.com careerist is one of the best online boot camps. Careerist graduates are already working in over a thousand different companies in 40 different states. And they're working in jobs related to the courses that careerist offers such as manual QA, sales engineering, UX design, systems engineering and data analytics. Now what actually sets careerist apart is it caters to people who have no prior tech experience, technical background or technical education. And they provide comprehensive training that equips you with the necessary skills to get a job in the tech industry. You'll attend live classes, get internships to practice your skills, receive personalized guidance from a career coach and receive support at every stage until you achieve your career goals. So thanks to careers for sponsoring this video. They are the go to solution if you're trying to get into a career like manual QA. And I do have a coupon code for 10% if you click the link down in the description as well as the pinned comment below. So overall I'm going to put manual QA into S tier. The next one on the list is going to be content marketer. So this is a new ish type of marketing position and it's gotten more popular in the last few years. And you've probably seen how billion dollar companies are starting to create content. Even billionaires are starting to create content. Just look at the all in podcast. And there's a reason for that. They're not just doing it because it's fun, although it is pretty fun. They're doing it because content marketing is one of the easiest ways to establish a brand presence, get leads, sales and make more money and content marketers specialize in this. And they also make pretty good money about $63,000 a year, even though this is still an entry level position. And typically in this position, they actually prefer younger people because younger people are going to be more familiar with what the current meta is in digital marketing. So this is another one I really like. And I think I read a survey that said like 20 to 30% of young people actually want to become YouTubers. So what better way to learn how to be a content marketer than to get paid to do it? So overall, I'm going to put this one into S tier as well. So I'm starting off with some good ones. There's going to be a lot of S tiers at the beginning, but I promise not all of them are going to be like that. The next on the list is going to be a paid media specialist. And this position might include paid search, affiliate marketing, social media advertising and more. And they make around $54,000 a year. So this is another one where you don't have to have a college degree or previous experience. They also probably prefer younger people that, you know, don't have that much experience. And it's overall a pretty good one. I'll put it into A tier. The next one on the list is going to be a front end developer. So this is a type of software developer and it's one of the easier ones to break into, but it's also one of the most saturated ones because of the fact that it's easier to break into. So basically you're going to be working on the front end of software or a website, which basically means the part that people can actually see. So I still think this one is pretty good. But realistically speaking, 10 years ago, you used to be able to get a job as a front end developer if you had a pulse and a warm body. Now you have to actually know your stuff. You really do have to teach yourself, whether that's going to college, self teaching, going to a boot camp and online course, etc. Plus in the last year or so, the labor market has changed a little bit and there has been a lot of layoffs in tech. And so for that reason, I still think this one is really good, but it takes a bit longer to get into than some of the other ones on the list. And it pays well about $84,000 a year, but there's some that you can get into and make that money faster. So I'll put this one into high A tier status. The next one on the list is going to be a mobile developer. So this is exactly what it sounds like. You're basically a software developer for mobile devices. And notice how much more money they make than front end developers. They make about $116,000 a year. Now it's probably around the same level of difficulty in order to learn the skills, but it's much easier to get a job because there's a lot less competition and saturation. So there's a lot of opportunities, for instance, if you learn react. So I'm going to go ahead and put this one in S tier. And by the way, if you disagree with any of my ratings, please let me know down in the comment section below. I like to get a conversation started because hey, even if we disagree, everybody can learn something from it. The next one on the list is going to be a DevOps engineer. And this is one of my favorite software development related careers. So DevOps engineer is kind of like software developer mixed with manual QA mixed with it. And their main job is to get things shipped fast. What that means in the technology world is getting a product from prototype to being able to actually be used by customers as fast as possible. Now this is not typically an entry level software development position. Usually you're going to have to have a bit of experience to get into this one. But of course there are exceptions. And DevOps engineers make over $120,000 a year. So this one is great. I'm going to put it into S tier. The next one on the list is a customer service representative. So this is a job that a lot of people kind of frown upon sort of look down on it. But the truth is, this is one of the easiest remote jobs that you can possibly get. And you can literally get this one in many states as a 16 year old, right? It definitely does not require a college degree or previous experience. And yes, I know it's probably not something you want to do for long, but it's a great way to get your foot in the door. And there's actually some pretty good jobs that you can naturally move into, especially if you become a customer service representative in the technology industry. And so this is not a bad first job to get, especially if you're trying to get a remote job. And with this one, you can expect to make about $39,000 a year. So overall I'm debating whether to put this in C tier or B tier. I think it'll go in low B tier. The next one on the list is going to be tech sales. I think everybody knows my feelings on tech sales. I've talked about it many times on the channel. The entry level career here would be BDR or SDR, which stands for business development representative or sales development representative. Absolutely love this career. Super easy to get into tech with this. The entry level positions pay around $70,000 a year, which is amazing. You don't need a college degree or previous experience. And technology sales in general pays $141,000. Plus there are even higher paying jobs you can get to, such as account executive. So yeah, this one is great. I will give it an easy S tier rating. Another really great one, probably the easiest job to land in the technology industry, if you're just trying to get your foot in the door, because it's easy to move around once you're in, is going to be IT help desk. Now tech sales and IT help desk are kind of tied in terms of how easy they are to get in. But the truth is tech sales, even though it's in the technology industry, is kind of more of a business career. So I would say IT help desk is the easiest technology career to get into the tech industry. And basically you're going to be answering technology, technical related questions for customers, as well as your coworkers. And you'll make around $53,000 a year. Now there's so many different positions you can move into from here. This is an entry level position, like I said, doesn't require a college degree, doesn't require previous experience. And there's many IT related jobs where you can make multiple hundreds of thousands of dollars a year. So I'll put this one into S tier as well. The next one on the list is going to be another great one. I promise I'm not going to put all of them into S tier, but this is going to be data analyst. So data analyst is one of the lower level kind of like an entry level data related position. And anything that is data related is going to be incredibly valuable. And basically you're going to be organizing and analyzing data in such a way where it can deliver actual results for a company. And data analysts make about $80,000 a year. So this is another S tier career really like this one. It is a bit harder to get into at the entry level. And there is a lot of argument about this one, whether you should get a college degree or not. But in the technology industry, if you have the skills and you can prove that you have those skills, that's all you really need. But a lot of people do get statistics or computer science degrees before they go into data analysis. But with that being said, I'm still going to put this one into S tier. The next one on the list is going to be a freelance writer. So I have kind of mixed feelings about this one. I do think there's a lot of good writing opportunities out there, but most writers don't actually take advantage of those. For instance, I think one of the best skills you could possibly learn right now is becoming a YouTube script writer.  They cannot find anybody that can properly research and write scripts because YouTube script writing is completely different than any other type of writing. So a lot of people fail when it comes to writing. It does make about $52,000 a year for the people who succeed, but a lot of people fail. But if you specialize, you go into the right thing. For instance, becoming a YouTube script writer or even better specializing in a particular niche, this one can be good. But overall, I have to give this one a C tier rating. But if you're able to specialize and you do the right things, this one can be a lot higher. The next one on the list is going to be a financial analyst. So this is one where you do have to have a degree to get into it, typically an accounting or a finance degree. And basically you're going to be analyzing financial data and financial analysts make about $87,000 a year. So the fact that you do have to get a college degree to get into this one is going to make me rank it a little lower. But with that being said, if you have an accounting or a finance degree, this one can be really good. I'll give this one an A tier rating. The next one on the list is going to be a legal assistant. And basically, you're going to be providing crucial administrative support to legal professionals. Now, in this position, you make about $50,000 a year and you don't have to have a college degree or previous experience, although some legal firms might prefer if you do. So overall, this is a pretty good one. I'll give it a C tier rating. Next one is going to be a wealth manager. Now, this is obviously not an entry level career. Nobody's going to let you manage their wealth if you don't have any experience. Unless you're Sam Bank from freed, of course, you also need to have degrees and certifications. But for that, you make about $170,000 a year. So pretty good, but it does have a pretty big barrier to entry. I'll give this one a B tier rating. Next one is going to be a travel agent. You're going to be helping people to plan and book vacation experiences and travel agents make about $53,000 a year. So overall, this one's pretty good. I kind of want to put it in high C tier or low B tier. I guess I'll give it a B tier rating. The next one on the list is going to be a virtual assistant. So this one is not as good for people from first world English speaking countries like the United States, but it's actually really good for people outside of that. And the reason for that is because very simply people from outside the United States are just as smart as people in the US and they can kind of do these same types of tasks. But there are certain types of tasks where it's just better to have a virtual assistant from the US and VAs from the US make about $44,000 a year. So overall, not a huge fan of this one. Of course, there are certain exceptions like executive VAs can be pretty good. But typically this is a position that does require some experience and you're not getting paid all that well. So I'm going to put this one into C tier. The next one is going to be digital marketer. This is another one of my favorite careers. You do not have to have a college degree or any previous experience, but you do have to have the skills and build a portfolio. And digital marketers make about $64,000 a year. So there's also a bunch of different types of digital marketers. There's SEO, which is search engine optimization. There's PPC, which is pay per click. So definitely look into those. But this is the one where, in my opinion, I see the most people going into it and actually enjoying their job. Plus, there's a lot of upward mobility. There's many marketing positions where you can make it into the hundreds of thousands of dollars a year range. And it's a great job to start out at if you want to start a business down the line. So I'm going to put this one into S tier as well. The next one on the list is going to be a data entry specialist. And I have to put something into F tier. So I think data entry specialist is probably going to be the one. I'm not even going to go over the stats very low. It's also mind numbing work. It can be really hard on your hands like you can develop carpal tunnel at a young age. You're also typically doing jobs that anybody else in the world can also do. And so why in the world would a company pay you like five times as much? But the one good thing about this is it can usually be done remotely. But that's not enough. I'm still going to have to put this one into F tier. The next one is going to be transcriptionist. So transcriptionist is also pretty bad. I will say it's pretty easy to land these types of jobs just to get started off. Typically you're going to start working either as a freelancer or just part time, but it is easy to land these jobs and start making money. So that and the fact that you can do it remotely are kind of the two saving graces. But with that being said, just about everything else is pretty bad kind of like data entry. It's extremely mind numbing work for instance. And a lot of transcriptionists will actually report that they will actually start typing things that people say if they're ever around their computer. So that's how autopilot they have to go in order to do this job. So some types of transcription are better than others. For instance, legal transcription is going to be a little better, but this is definitely not something you want to do forever. Maybe do it for an entry level job just to get a bit of experience. But overall, I'm going to put this one into D tier. Alright, so I got to speed up so I can't go over the salary or anything like that. This video is running too long project manager is next really like project manager kind of like entrepreneurship with training wheels. It's not an entry level job. Typically you do have to have some experience before you go into this role, but you do get to develop your leadership and management skills and you get paid pretty well. So I'll put this one into a tier next one on the list is going to be operations manager. This is where you manage the operations of an organization, which is incredibly important, especially when your business starts to get bigger. It's definitely not an entry level role. You're going to have to have some experience, but I really like this one as well. It pays really well. You can do it remotely sometimes. So I'll put this one into a tier as well. Next, I'm going to talk about cloud related jobs in general. So basically jobs where you're working when Azure or AWS, which is Amazon web systems, these are extremely hot right now. So I mean, these are some of the easiest jobs to land and also some of the easiest jobs to make really good money with. So I'll put this into S tier next is going to be appointment setter. So if you're somebody who wants to start a business down the line, especially kind of a cash flowing online business such as an agency or a consulting firm or selling courses or selling coaching, anything like that. Appointment setter is probably one of the best careers you can possibly start out in. So if that's what you want to do, appointment setter is a great way to go. I'll put it into S tier. Another really good one to go into if you want to go into one of those online businesses is high ticket sales. So basically you would be what's known as a closer. The appointment setter is the one who sets up the appointments. A lot of the time they'll do that through DM. Sometimes they'll do cold outreach, calling people. And then sometimes they'll do really quick appointments just to make sure that the person is qualified. And then they pass them on to the closer, which is the high ticket sales person. This is another really good one probably not for everyone because it is kind of a high pressure position, but you can make even more money than you can as an appointment setter. So this one is also going to go into S tier. The next one is going to be a customer success manager. And this is a position that's getting more and more common. So usually you're going to be working in either a high ticket business or a B2B business. That's a business that sells products or services to other businesses. And you're going to be working very closely with your clients to make sure that that product or service is serving them correctly. So you're almost like their personalized consultant for that product or service. So really good position. I really like this one. I think I'm going to put it into a tier. And then the next one I'm going to talk about is content strategist. So this one is going to be great for you if you're somebody who eventually wants to start a YouTube channel or some kind of online brand. And it's exactly what it sounds like. You're basically coming up with strategies to make your content do really well or make the content of a company do really well. So I do really like this one. Right now it's kind of the wild, wild West. There really isn't an infrastructure to become a content strategist. So you kind of have to get creative in order to get a job in this role. So for instance, you'd probably be cold emailing different businesses as well as content creators out there. And that's how you'd be able to get your first job. So the best way to become a content strategist in my opinion is to just start making content yourself. Other people might see your content think it's really good. And then they might want to hire you. So because of the fact that it's a bit hard to get into and there's not really an infrastructure here, I'm going to put this one into a tier, but it definitely could be an S tier career in the future. Now if you like this job, check out my best remote job tier list where I go over a bunch of other careers. And you can check that out by clicking right here.


Thank you, kids, for sharing. That was so great. Parents, you can pick them up in the banquet hall after service. We're going to be in there. And the rest of the kids are in the other building. But the choir kids will be in the banquet hall. Please, we have a special treat now. We have the play coming. And we're also going to be having a little video presentation to kind of give us a glimpse of what the play is going to be about. So we direct your attention to the screens. Thank you. God bless you guys. Welcome to Cooking Up Christmas, where love is the main ingredient. This year, the annual choir Christmas party is stirring up something new. Instead of the party being all about them, the kids have collected favorite recipes from their church members and are cooking up some tasty treats to share with the community. As they collect recipes, cook them together, and wrap together, working as a team, they'll learn all about the real recipe of Christmas. This is sure to be. I want to thank you all for your participation in the collection of the recipes. Looks like we're going to have some really great ones. Can't believe she's sending the recipe. She tries to dish it up for us at every family holiday. The bird dog said something. I just tried to disappear in the pie popsicle. Did you know that popsicle? Looks like we have lots of other recipes not to worry. Hey, everybody, I want you to meet my new friend Bobby Gray. He just moved here. And he's never been in a choir before. Come on, everyone, let's say hi to Bobby. Hola. Welcome, Bobby. I sure am glad to have you here. We're going to be cooking up some fun songs and some good-tasting goodies this Christmas. Yes, I can't wait, but awesome pie. Is this really welcome all new people to your choir? Now, we're going to a long choir. Today, we have two special guests with us. And they've cooked up a fabulous recipe for our annual indescribable, you won't want to miss it, choir Christmas party. I believe you all know Miss Betty Rocker. Say hi, everyone. Hi. And now, for our extra special guest, please welcome Mr. Emerald Lasagna. You know that he has his own cooking show on TV. He's famous. He's famous all right. I want to show every chance I get. He's inspired me to be a chef. Maybe one day, I might have my own cooking show. Rachel, I didn't know you cooked. What's a specialty? Possum pie. Oh, no, Christmas cookies are my specialty. Hi, kids.  Excuse me, Mr. Lasagna, there'll be no kicking of any kind in my kitchen. Yes, of course, Mrs. Rocker. Yes, ma'am. I also hear that Mrs. Rocker has been in charge of your kitchen for 22 years now. Excuse me, Mr. Lasagna, that's 25 years. Boy, she does take her job seriously, huh? Oh, yes, she does. Don't even try to sneak into her kitchen. It's not going to go off as crack, because it's reserved for the church and the city. She'll have you on travel to her congregation. OK, everybody. Here's the deal. Instead of having our normal choir Christmas party. Oh, normal? We don't do anything normal around here now, do we? We do everything big and always with a song. Yes, sir. But always a photo traditional Christmas choir party. It's a good thing. As I was saying, we would not just be making these recipes for ourselves by sharing them with the neighbors in our community. This choir Christmas party is all about the main ingredient of Christmas. That's right. This year's choir Christmas party is all about cooking up Christmas. Guys, ready to decorate some cookies and wrap some presents? Yes, ma'am. Looks like there's going to be some Michael Madge right here. Mrs. Goodhouse Team Awards is front and center. There'll be no fooling around in her kitchen. Mr. Badie and Aileen Humding isn't here to butter up with some of her what was that? A real pot pie? That's absolutely awesome, possum pie. Not Armadillo, Martha. Ben, hey, everybody. Are you ready to cake this Christmas party up a notch? Now, you just remember, Mr. Lasagna. There'll be no kicking in my kitchen. Part of that we're not doing our normal traditional Christmas choir party, but I think this is such a fun idea, cooking together, working together, and reaching out to our neighbors. If we really try, we could probably get it all done in 30 minutes or less. While reaching out is one thing. But somehow, this year, it seems as if you're making Christmas about everybody but us. Get it? Christmas? Everybody but us? Oh, I don't know, whatever. I still can't believe I'm in a church kitchen. I mean, Ms. Badie's rocker's kitchen. I was out of the morning, eating a food for strangers. I always thought Christmas about me making a noise calling stuff and eating a lot of food for me. Actually, I do the true origin of Christmas. I'm fine, you can learn all about it in the first two chapters of Luke. Luke, who's who? Is that like Vader's son? No, silly. Luke is the book found in the Bible, and that's why I find the whole meaning of Christmas. The Bible, don't think you've read that book before. How much we miss you something? Hey, if you want, I can tell you the story. Sure. Hey, you guys, come on over here. Guys, can I tell a story well or kicking, cooking up Christmas? I'm gonna tell that angel, Gabriel, to find a sweet young girl named Mary and tell that she was gonna have a baby. Now, Mary was just a little bit confused because she wasn't even married to Joseph yet, but Gabriel told her not to worry one little bit. The Holy Spirit had it all figured out because Jesus was God's son. Then Gabriel just slipped right into Joseph's dreams and told him all about the miracle, told him all about God's plans. Now, right close to the time when Mary was supposed to have a baby, somebody in the Roman government got the bright idea to count everybody. Well, that meant that Mary and Joseph had to make a road trip to Bethlehem, and when they got there, there were so many people ahead of them that the only place for them to stay was in a stable, and it was there, in that lowly humble place that baby Jesus was born. Now, if that wasn't exciting enough, this is where our miracle recipe really gets spiced up.  The angels were singing and everything, and the shepherds were very scared, but they rushed right off and found Mary, Joseph, and baby Jesus, just like the angels said they would. Jesus, the only son of God, had come to earth.  Gives me chills every time I hear it or tell it. Well, guy, you certainly had a different spin on it, and Luke, it's not quite as colorful. I don't think your version would come up on Google. Yes, but just as I'm using this recipe to make this soup, God had a recipe in his heart because he promised his savior years and years before, and he did just that. He sent his only son, Jesus, into this world, and he is the main ingredient of Christmas. This amazes me to think of how God said Jesus was born in a lowly, humble major, sleeping on hay under all those twinkling stars and all those animals and all those angels and the heaven singing so bright always amazes me. I never heard a story like this, but it sounds like a good done on camping time, too. I wish I'd been there. I've heard this story all my life, but I just don't understand why God said, why God had sent his one and only son to be born in a major in the middle of a stinky stable. I mean, wouldn't you think that God's son would have a king's suite at one of Bethlehem's finest hotels? I mean, just imagine. A starry fireworks, a catered buffet, and the heavenly house would be in charge of the music. Now that would be a grand entrance I would think he deserved, not a bed in a manger. Yes, you would think that, but that is exactly the whole point because God knew about everyday living, and when he sent Jesus into this world, he wanted us to know that Jesus lived life just like us. The difference was that his everyday living didn't cause him to sin because he is the son of God. Always amazes me. This makes me want to love and serve him more. It makes me ask myself, what gift can I give him? What can I offer him now? Hey, Spina, I guess we gotta wrap this up and get these presents to the community. Thank you, Bobby, for coming out to help out the church choir on a Saturday morning, even though you just moved here. Well, at first I wasn't sure about this kicking up and cooking up Christmas stuff, but after hearing that Luke story, I know how to put this, but I wanna believe like you guys do. That's great, Bobby. That's great. Yeah, awesome, awesome. I know what you're saying, Bobby. I've never missed a choir Christmas party. All vacation Bible school, they'll come to think of it. I have snuck some goldfish crackers out of the church nursery more than once, but even with all the parties, foods and presents, even I have truly forgotten the true meaning of Christmas. The Bible says that all you have to do is confess your sins to God and believe in your heart truly that Jesus is your savior because God gave his great gift of love for all of you. And that is why he has been made a greeting of Christmas. Wow, so Christmas is about us after all. Just not in the way I thought it was, huh? Yeah. Hey, everybody, Luke's who's here. Aunt Erlene, and she's brought her absolutely awesome, awesome pie and it looks like there's enough for everyone. Yeah. Hey Aunt Erlene, these cookies sure do smell good. Which come on and have some with us. We'll save for that pie for someone really special, like, I don't know, Pastor David. This is just great. Hasn't it been so much fun? We've been cooking up Christmas and I learned all about the main ingredient, Jesus. And now I'm gonna share with the neighbors in our community. Let's do this. Yeah.


Hello, so in the previous lecture we took a look at the importance of targeted proteomic approaches in the field of proteomics, the different steps that are required to design a successful targeted proteomics experiment and the principle and basics of selected reaction monitoring approaches. In the lecture I had specified that we would use the software skyline to analyze a data set. So today's session would focus on the use of open source software named skyline to analyze the data set from a selected reaction monitoring experiment that was done in our lab. This software was developed by Mr. Brendan MacLean from the lab of Dr. Mike MacCos at the University of Washington. The software skyline has revolutionized the field of targeted proteomics. It is a very simple to use software and there are many tutorials which are available on the website. In order to use skyline you are requested to register and download the software from the following link. So using a data set from an experiment that was performed in our lab we would quickly walk you through the different tabs that are present in the software skyline and how to look and analyze the data that was generated. So before we look at skyline and the different options available I would like to briefly discuss the experimental design. So the protein of interest for this particular experiment was apolipoprotein E which was found to be significantly dysregulated in one of the projects. So for this experiment we had ordered heavy peptides from a vendor and the heavy peptides are nothing but the same peptides which one wishes to monitor in their sample of interest but they differ from the endogenous peptide that is the peptide that is present in the sample in the presence of a heavy labeled isotope at the C terminal end. So it is clear that these peptides would contain the same amino acids and hence their chromatographic behavior will be exactly similar to the peptide of interest that is the endogenous peptide or commonly referred to as the light peptide in this case. So the heavy peptide helped us first get an idea of the retention time of our peptide. So once we knew that the peptide of interest would elute at a particular time these heavy peptides were spiked in our sample of interest and we monitored three peptides for the protein apolipoprotein E. So let us now look at the software and the different options that are available. The first and the foremost thing that we need to do is go to the default settings and say no so that it removes any previous setting which was there in the software. Once this is done the particular document is fresh and blank. So this is where you paste the targets. So in this particular experiment we had chosen three peptides of interest and these were the sequences for the peptides. So I go to I just copy these three sequences I go to edit say insert and peptides. I paste these three sequences here. So what you see is each of these peptide sequence pasted and I say insert. So to your left you see three peptide sequences for the protein apolipoprotein E. Next look at the settings tab. In the settings tab you to go to peptide settings start with the digestion when you specify these specific parameters the enzyme use the maximum mixed cleavages specify if there is a background proteome. So in case if you have the proteome of your organism you can always integrate that with skyline and all the process which I showed about pasting the peptide sequence can be avoided. So once a proteome has been chosen you can simply type the accession number of your protein of interest here and skyline would generate all the different peptides that result from in silico digestion of the protein. Once this is done we move to the prediction tab where if you have a prediction a retention time predictor you can use this calculator to predict the retention time of your peptide of interest. In this particular experiment we did not spike in anything so we have left these values default. Then we move to the filter tab wherein you specify the length of the peptide that is to be monitored. So it could be from 8 amino acids to 25 amino acids though the best peptides usually range from 8 to around 16 or 17 amino acids in length. Then skyline also allows you to choose any peptides which you would want to exclude due to their amino acid composition. Then we move to the library tab. So the use of library is that it would help you in data analysis by comparing it with the library spectra. So assuming that you have performed a lot of shotgun experiments after analysis of the shotgun data the files can be used to create a library of all the spectra that were detected in the shotgun approach. So once you integrate the library spectra into skyline and then you generate targeted data you can always compare the library spectra match and then skyline provides a score to the match between the spectra in a targeted experiment to the to that seen in a shotgun experiment. So you might not be able to see any library here because you have just installed the software but in my case I see a human LFQ library because I had used it in one of my experiments. So we next move to the modifications tab wherein all the specific modifications which might occur during sample preparation can be specified and this particular tab is of importance here. So it specifies the isotope label type. So we have used a heavy peptide as I have mentioned previously and the modification for that was this. So I have selected this particular modification however if there is any modification other than what I have specified you could always go to the edit list tab and add the modification. All the details for this software have been very well explained in the tutorial section available on the skyline website. Then the next tab contains all the quantification related values in this case our idea was not to quantify but to just look at the correct peptide in our sample. So we left all the values as default. Once the parameters have been changed you click ok. So you can see that the peptides now have a blue amino acid here, amino acid labeled in blue. This indicates that the heavy label was present at this site. We again go to the settings tab and we go to the transition settings. So this is where we generate the different transitions for the peptides and the different charge state of the of the peptides. So we left all the values default here for the prediction tab. Then in the filter tab skyline allows you to specify the precursor charges and the ion charges and the ion types. So in a typical experiment you would want to monitor precursor charges of 2, 3, 4 and ion charges of 1, 2, 3 and different y ions, b ions and some other ions which you wish to monitor. For our experiment however we had kept everything very simple, we monitored only a plus 2 precursor charge and then a plus 1 ion charge and only y ions do not complicate the matters. Here the special ions you can also specify the special ions moving to the library tab. Since we do not have a library incorporated we could just deselect this option. In case you have a library which has been incorporated selecting this would let you choose the peptides and the fragments. So if you select this it would give you, you could always specify how many product ions you need to monitor. Once that is over we go to the different instrument parameters, we left the parameters as default here and the full scan. So this also is a, also is default and we clicked ok. So I would just like to put light on this particular aspect. If the idea is to go for a DIA approach, Skyline helps you use this option of DIA. If however if you wish to do a PRM method you could go to targeted and choose centroided as the product mass analyzer. So since this method was a SRM method we say none and we click ok. So now we have successfully made the settings at the peptide level and the transition level. So if you look at the left side of the, of your page you see that there is a drop down menu, drop down here when I click on this plus symbol it gives me two charge states here or two mass to charge ratios here. One is 865.9258, the other is 870.9299. So the one which is of a higher mass to charge is for the heavy labeled peptide which we had selected and it is written in. So you see that there is heavy in the brackets and similarly for the other two peptides. On the right hand side of the page, the right bottom side you see that one so you see that there are a total of 22 transitions coming from 3 peptides and 6 precursors. So this is the basic information that you see after creating your file. So once this file is generated you need to save the file, let us say save the file as, now once these file has been saved we can export this as a transition list. So I specify the instrument type that is TSQ the alt is here and then I say use start and end RTS and say okay, so default collision energy yes and then this I say transition list. So now Skyline has actually exported the entire list if you can see this. As you can see here Skyline has exported each of these peptides and its corresponding transitions. So the precursor is the Q1 value, the product is the Q3 value, the collision energies, the start and the end times for the experiment. So this was the transition list, however if you export this method as an isolation list then the resulting file is that for a PRM. As you can see there is no mention of the sequence information, all that the method says is the mass to charge which is the Q1 value, the charge state of the peptide, the parent ion, the collision energy that is to be applied. So for a PRM experiment all the data will be captured that is all the fragments which get generated after fragmentation of the parent ion will get captured. So assuming that the experiment has been performed, I now quickly import the data which was generated from this experiment, the results I say okay and these are the same files which were run in triplicates I say okay. So Skyline has now imported all the files and if you see this, let us go to a different view for this I go to tide. So as you can see each of these samples you see the intensity for the peptides in each of these samples. So for the peptide 1 you see that the total ratio is 0.005 which means that the intensity of this particular endogenous peptide as compared to the heavy peptide is the ratio of 0.005. So the peak, so currently I have selected the information for the heavy peptide and when I click on the sequence per se what we see is this is the synthetic peptide that is the heavy peptide and this somewhere here is the endogenous peptide. So if I zoom in, I will get to see the endogenous peptide peaks as well. So this is the endogenous peptide and this is the heavy peptide. So the next step in the data analysis involves looking at the transitions which have been detected and deleting the ones which have not been detected. So in this particular case I have not been able to see the transition for E6, so I just remove that. Here I do not see the transition for Y6, I remove that. So this is the very basic information that can be provided to you about the use of Skyline. So this was a very basic session on Skyline and the different capabilities that the software has. I am sure that you are now interested to explore this software even further and get a much better and a comprehensive idea of the power of targeted proteomics. In addition to Skyline there are many other vendor specific softwares which can do the same job. We request you to take a look at the different software options that are available, try out a few test data sets and get a better understanding of targeted proteomics experiment. I am sure that now you are interested in getting a much better understanding of targeted proteomics approaches and how they are likely to help you better your research. We wish you all the very best in exploring this exciting new field of proteomics. Thank you.


This is stock number 11074. We are here at Summit Automotive in Fond du Lac, Wisconsin. You're new and used heavy-duty truck and RAM headquarters. Today we are checking out this super clean 2018 RAM 2500 Crew cab short box SLT. This truck has the 6.7 liter Cummins diesel. It has been fully safety and inspected by our service shop. It has a fresh oil and filter change. All the fluids have been checked and topped off and this truck is 100% ready to go. Blue streak metallic is the color we shoot all of our videos in 1080p 60 frames per second. So if you have HD capabilities on your computer tablet or smartphone device turn them on right now because it is your best way to Check out the quality and condition of the truck before seeing it in person. And if you'd like to check out all the photos on this truck in the upper right hand part of your screen as a link to our website Click that and check us out there. This one comes with some XD series 20 inch painted and polished aluminum rims. It has Goodyear Wrangler Dura-Track tires on here. These are 35 by 12 and a half R20 LT's and they have just about all the tread left. I'd say probably 90% or more. You can still see the little wear knobs on there a little bit So I would say probably 95% or more tread left on those tires. Framon underbody is in really nice clean condition and it does have a suspension lift kit on it. Front bumper, I will say that this does have aftermarket iron cross front and rear bumpers and That is why the parking sensor lights are on because these obviously don't have the parking sensors. So when we get inside you will see that. LED fog lamps headlight lenses are nice and clear and it does come with a smitty built X2O winch on here. Not really sure the exact specs on that winch, but it's in pretty nice shape. It is a metal winch or metal cable I should say. Hood is in nice shape. No major dents or dings on that. Passenger side front fender is in nice shape as well and the passenger side rim is in excellent condition too. I wouldn't doubt that these rims and tires were both brand new when they were put on this truck with how nice the rims look and with how new the tires are. They were probably put on at the same time. As you go down this side of the truck take note of how clean the body is, how reflective and mirror like that paint is. We take these HD videos, so if you are far away or even if you're close by and you just cannot make the trip down, but you're still interested in purchasing the truck, you can see the truck, hear the truck, and have confidence in the vehicle that you're looking at before you even get here. So then when you do get here, there's absolutely no surprises. And that's why we offer the Summit Auto Fastpass option in the upper right hand part of your screen. A link right to our website where you can apply for financing, get pre-approved, see a payment quote, even appraise a trade, all from the comfort of your own home. So when you do get here, there's absolutely no surprises. The frame and underbody is extremely clean here and it does have all the remaining factory exhaust, so it hasn't been altered in any way like that. It has the nice chrome step bars and as we come around to the back of the vehicle rear bumpers in really nice shape. It does have a full towing package which includes a receiver hitch, four pin and seven pin wiring, and the tailgate is in really nice condition as well. It has a very nice spray and bed liner and you do get a gooseneck hitch back here. The only thing I saw is one tiny little ding on that tailgate there, but other than that, very, very nice as you go down the driver's side just as clean as that pass on your side. No dents or dings in the box and for full disclosure, this back rim is very, very nice as well. Down the rest of the side of the truck looks really good. Didn't see any dents or dings in the doors. It does have the heated power mirrors with the built-in directional signals. Inside the SLT package gives you the gray cloth interior. You do get the bucket seats on this one. No rips or tears on those seats, power driver's seat with lumbar, factory floor mats throughout, power windows, power locks, power mirrors, and these mirrors do power fold in. I was like showing both sides that you know all the features they're working on the truck. Auto headlamps and as we hop inside the vehicle here, turn the ignition on so that you can see the miles and everything. This one has 18,969 miles and like I said it says service park sensors because it doesn't have them in there anymore because of the bumpers. Digital speedometer, outside temp and compass display. You get these seven inch LCD display up there. Looks really good, nice and clean. 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And then you can check out your backup cameras here so you can either do the regular backup camera or you can do the cargo cam which is pretty nice and those are pretty seamless to switch swap from and you can turn them off as well. Down here you have your dual climate controls, the more tactile buttons, the volume and tune controls, turned out four wheel drive, factory brake controller, stability control to a halt. That's your factory exhaust brake and if you did have those sensors in the bumpers you'd be able to turn them off and on but they're permanently off of course. 115 volt, 150 watt plug-in, passenger side format and seat are in really nice condition as well. No rips or tears on there and the headliner is in really nice shape too. Those come with side curtain airbags, map lights, power slide rear window and your home link buttons for your garage door security systems and lighting systems. Assist and SOS buttons in that rear view We'll take a quick look at the back seats and then we'll start it up and take a look under the hood. Back seats are just as clean as the front seats, no rips, no tears back here. It does have the latch child safety system for any child car seats you may want to put back here. Power slide in rear window.   You also get storage underneath the seats, underneath the floor mat here you got all weather or I'm sorry removable containers for easy cleaning you can put ice and drinks in there it's like you have too many coolers wherever you go and the backs of the doors look pretty good you also get the child safety locks bottoms of the doors look really good as well and just a really really nice truck all the way around we'll start it up see other than the park sensors there's no check engine lights or anything and we'll take a quick look under the hood we do have the controller for that winch as well that's inside the truck in the center console and I would personally like to thank you for checking out the video today and hopefully from this hd video you'll have been able to tell this top clean this truck is all the way around the inside up under the hood you get the 6.7 common diesel engine base very clean runs very smooth once again this truck has been fully safely hand inspected by all the service shop has a fresh oil and filter change all the fluids have intact and topped off and this truck is 100 percent ready to go I would highly recommend it from a quality and condition standpoint and to see more pictures of this truck or one of our other 450 new and used cars trucks scvs minivans wranglers half tons two cotertons one tons you name it we got it go to our website right there www.summitauto.com full pictures and descriptions of every single vehicle from two locations all at summit auto.com and if you'd like to check out more hd videos you can go to youtube.com slash summit auto remember to like subscribe and share on this video and all the videos that you see there in fact in a second you can see link to subscribe to our youtube channel on your left links more ram 2500 truck videos like this one on your right you've not been to our website on the bottom link to this vehicle on our website click those check us out and we're super excited to be offering this super clean 2018 ram 2500 true cab short box slt and blue streak pearl thanks again for checking out the video


Hi, everyone. This lesson is on the power role for finding derivatives. So if you think back to what you've learned so far about finding derivatives, you've learned two limit definitions that we refer to as the first and second limit definition of a derivative. What we have here is the first of some shortcuts or alternatives to finding derivatives, alternatives to using those limit definitions, and this is the first of them. So what the power role states is that if f is a function such that f of x is equal to x to the n, where n is an element of the rational numbers, remember q is our mathematical symbol for the rational numbers, and remember what a rational number is, is any number that can be expressed as a fraction. Then f prime of x, the derivative, is equal to n, which was the exponent, times x now raised to the n minus 1 power. So let me give you a really quick little example. So if we had a Quintic equation, 2x to the fifth minus 6x squared plus 6x minus 12, the derivative you would have y prime is equal to, so you do 2 times 5, you do the coefficient times the exponent, that's 10, x, and you subtract 1 away from the exponent, so now it's 4 minus, now you do 6 times 2, that's 12, x, take 1 away from that exponent, so that's just to the first. If you like to write in the one exponent, please do feel free, you might find it helpful for a little while. Plus, now remember the exponent on this linear term, the x is simply 1, so you have 6 times 1, so that's simply 6. When you take 1 away from the exponent, of course, you have now x to the 0, which we know is 1, so it's really just 6, okay? And then the constant term minus 12, that really just goes away, and we're going to talk about y in a second. So what we have here is a really quick way now to get the derivative. Now think about how you would have had to do this before. This was just an equation that you were asked to find the derivative of, so we probably would have had to use that first limit definition of the derivative, the one with the h in it, and remember how that numerator goes, f of x plus h, so imagine having to figure out, you know, if you substitute x plus h in there and have to do that to the fifth power, you go ahead and do that if you really feel like it. But that's why these rules, and as I said, this power rule is just the first of a few that you're going to learn shortly. They really are shortcuts if you want to think of them that way. So let's talk a little bit more about why this 12, when we took the derivative of that, that constant term, why did that come out as 0? Now there's two different ways you can think about this, and we're going to use that 12 as our example. So that constant term was a negative 12, and a constant term we know has obviously no x attached to it. You could think of it as x to the zero power, and x to the zero power we know is 1, so really you just have minus 12. Well, apply the power rule to that. If you do negative 12 times zero, obviously that's zero. You have x to the negative one, which would be zero over x to the first. Well, zero over that's just going to be zero, assuming of course x is not zero itself. So that's really one reason why when you take the derivative of a constant term, it just comes out zero. Now let's look at it from a geometric point of view. So let me draw a set of axes, and I'm going to think of that negative 12 as an equation, y equals negative 12. So these are my axes. So let me go ahead and graph that if I think of it as y equals negative 12. Well, we know the graph of y equals negative 12 is simply a horizontal line down here at negative 12. So let me put it right about here. Well, it's a horizontal line, right? What is the slope of any horizontal line? Zero. All right, so really what you have here are two different interpretations to explain why the derivative of a constant term is zero. You can take it from an algebraic point of view, which utilizing the power rule, you can also take it from a geometric point of view in terms of thinking of the derivative as the slope of a line tangent to a curve. Well, if this is a horizontal line, the slope anywhere is going to be zero. All right, so you really do have two different explanations, if you will, as to why the derivative of any constant term is always going to be zero. So let's take a look at some more practice problems, and we'll just practice using the power rule. All right, so the first one is g of x equals cube root of x. Now one thing you might want to do, think of this as written with an exponent, an exponential form. Because if you think about how the power rule goes, you're multiplying that exponent times the coefficient. So if you can think of things in terms of having an exponent, it's much easier. So the coefficient is obviously just understood to be one, so when you find your derivative, it's simply going to be one-third x, and now you have to subtract one from the exponent, so that now gives us an exponent of two-thirds. Now let's try the next one. So we have four-fifths times two is eight-fifths, t, subtract one from your exponent, and your exponent is just one. Again, if you find it helpful to write the one exponent for a little while, while you're still learning this and it's still new to you, feel free to do that. Now the next one, we'll rewrite that also in exponential form. So that would be two x to the one-half. So our derivative, we have two times a half, which is just one. Again, if you find it helpful to write the one there, please do feel free. You have x, take away one from the exponent, and that gives you a negative one-half. You're free to leave it like that, or if you find it preferable, you can rewrite it in terms of a positive exponent. Either one is fine. So let's try a few more. So we're going to rewrite this one also. So that would give us one over two. This would be x to the two-thirds. All right, but think about how the power rule goes. It needs to look like a coefficient times x to the n. All right, so we might have to rewrite this a little bit better. So let's rewrite it as the coefficient one-half, but then x to the negative two-thirds. Because your expressions need to look a certain way in order to apply the power rule to them. You need coefficient x exponent. All right, so that's really what you need to make sure everything looks like in order to correctly apply the power rule. So this derivative, we have one-half times two-thirds. That's going to be a negative one-third x. Now we need to subtract one away from our exponent. So that's minus three-thirds. So our new exponent is a minus five-thirds. Again, it's perfectly acceptable to just leave your answer like that. Now in the next one, we might want to rewrite the first term. We could rewrite that as a coefficient of one-half and x to the fourth. Now as you get better at doing these, you very well might find you don't feel it necessary to rewrite some of these. You can just do that in your head and that's perfectly fine. But starting out, it might help you visually to just rewrite them. So this derivative, we have negative one-half times four. So that's negative two. x take away one from the exponents. So that's cubed. Plus, now we have three times three is nine x and that becomes squared. And then the derivative of the last term simply is two. Let's think about this for a second really quick. Think of it geometrically. If I asked you to graph the equation y equals negative 2x, we all know that's a line through the origin with a slope of negative two. Hence the derivative is negative two because the derivative is slope of the line tangent to the curve. And if we had an equation y equals negative 2x, the slope is always negative two. So we can also think of something like that from a very graphical point of view. Let's look at a couple of other examples. Now this might be a problem in which you would have used the second limit definition of the derivative because we have an equation x to the fourth minus two and we are asked to find the slope, the derivative, at specified x values. So this would have been a good candidate for the second limit definition of the derivative. But let's try using the power rule. We will want to go ahead and find your derivative first. So let's do that. And this is a pretty easy one. We would just have f prime would be 4x cubed. And that's really it because remember the constant two will have a derivative of zero. So all we're really doing now is evaluating this derivative at all these different x's. That's it. So the first one would be f prime of negative one that we want. So that gives us a negative four when we substitute negative one in place of our x. The next one is f prime of zero. So that's just going to be zero. Now think about for a second what that's going to imply. And we're going to look at the graph in a minute. If that has a derivative, a slope of zero, that means it's a horizontal tangent line. All right, so we'll see. Check that out in a couple minutes on the graph. And then the last one would be f prime of two. So we have to cube the two. So that's eight times four is 32. So think about what that's going to mean. That's a pretty steep slope. So that's telling us that x equals two on the graph of this original function because we are talking the graph of the original function x to the fourth minus two. That's going to be pretty steep there. So let's go check that out on the graph and see if it actually looks that way. So I have entered in y one x to the fourth minus two and I admit I already played with the graph a little bit to get a nice one for us. So these you can really check it very easily by using your calculators built-in functions. If you do second trace and that number six dy dx, let's do the first one negative one. Remember we got negative four for our derivative and there it is. Now notice again visually what's happening there at x equals negative one. Notice how the curve is decreasing. Remember you always look at curves from the left to the right and at this point at x equals negative one. That curve is decreasing right there. Hence a negative slope. So let's try x equals zero. Remember that's the one where we got a derivative of zero and there it is. And lo and behold it is a minimum point on this graph where we have a horizontal tangent line. So let's try that x equals two. Remember that was the one that had a really steep slope to it. And there it is and notice how steep it is there. It's definitely increasing. Again looking at the graph from the left to the right. It is increasing and it is pretty steep there. Now you'll notice you got a decimal 32.mini zero's eight. That's because when your calculator does its derivatives it's not quite using the power rule. It's using some other numerical approximations of the derivative. So you can almost think of it that your answer is more accurate. So that's one thing to keep in mind. So that's why the calculator's looking a little different than what we got. So let's go back and look at one final type of problem. So now we want to find an equation of the tangent line to the function at the given point. We looked at problems like this before when we did the limit definitions. But remember we'd have to go through the second limit definition in order to obtain the slope, the derivative, at that specified point. And then we can use point slope form of a line to actually write the equation. So now it's just a little bit faster because we have the power rule to use in order to get that slope. So if we apply the power rule to this we would have y prime equals, that would just be 2x minus three for our derivative. Now our slope is going to come from doing y prime at one. So if we substitute one in for our x we end up with a negative one. So remember that gives us our slope. So now we can substitute into point slope form of a line. And remember how that goes. It's y minus y one equals our slope m times the quantity x minus x one. So here we would have y minus zero equals negative one times the quantity x minus one. Y minus zero of course is y. You could easily distribute that negative one if you wish. So we have negative x plus one and that is the equation of the tangent to the curve at that specified point. So let's try one more of that type. Feel free if you'd like to pause this and try it on your own first. We'll begin by finding our derivative because that's how we'll get our slope. So in this case our derivative is 3x squared minus 4x substitute in the x value of two. So we'd have three times four minus four times two. So that gives us a slope of four. So that's our slope that we want to use. Once again we can substitute into point slope form of a line. So upon substituting we would have y minus one equals four times the quantity x minus two. You can distribute that out and put it in slope intercept form if you wish. You get four x minus seven if you do that. But remember according to the way in which the AP exam is graded it is perfectly acceptable to leave it in point slope form and stop there. So the option is yours.


HBCU Dutchess Radio, welcome back. We are continuing our coverage of the COVID-19 pandemic and response. I'm your host, Jared Carter, and we are next up with our next distinguished HBCU president. He is the distinguished president of Morehouse College. Dr. David Thomas joins us again. First, thank you so much for making time for us, brother president. I would be remiss without asking how are things on the campus in Atlanta at large with the numbers seem to be, if it's even possible, moving beyond the realm of scary at this point. On campus, things are, and by the way, thank you for having me on the program. On campus, things are well. We've evacuated all of our students and only essential faculty and staff are present each day. And we started our online program. We've moved everything online and that's going very well. So in the context of where we are today, we think Morehouse is in pretty good shape. Good shape, and that's the thing. Morehouse has been at the forefront of a lot of decisions on cancellations and moving things online and providing safeguards for faculty and staff. But one of the unique things about you is that you made a call to not cancel but reschedule commencement for December. So what were some of the things that led you on the path to do something a little bit different from what a lot of HBC has done which is to say, we do have a date for this into the future. What was it, or what were the conversations like with your stakeholders to make that call? Yeah, great question. It's very simple. And, you know, my original thought was that we would simply push this year's commencement to next year and have a double commencement. But I thought about leadership at Morehouse and our student government and class presidents really provide leadership here. So I basically called the head of our student government, the head of our senior class or the president and talked to them about it. And they talked about the need for this class of 2020 to have their moment. And that then led us to look at when could we do that? And that's how we came out with on the weekend of December 11th through the 13th, we will have the graduation for the class of 2020 because they thought it was really important for that class to have its moment. And it's an important class for Morehouse because they've actually had five presidents in four years because they came in during a presidential transition and then we had several interims and ultimately I arrived. So it's a class that's really been with Morehouse through kind of ups and downs and they really stuck with us. So that's how we arrived at the fact that we need to give them their moment. It makes a lot of sense. And for Morehouse in particular, because of what you talked about with the transitions and with your arrival, you guys wanna on a really strong run of fundraising and partnership building and positive coverage in the media. And even a pandemic doesn't change what Morehouse means to all of us, even those of us who didn't attend, that doesn't change. But are there ways that you and your team and the brothers who are graduates and the students continue to engage your donors, your foundations, your partners when you don't have the benefit of saying, hey, come to campus and participate in this or be with us at Crown Forum and all these other things when you don't have that typical Morehouse infrastructure, you don't have the travel that you can do. Are there things that you think that you can keep doing to keep that mystique going that help raise a lot of money in a short period and help galvanize a lot of support? Yeah, another great question. Well, first of all, let's start with the fact that we are not operating from the assumption that there will cease to be a time when 2,000 plus young men are here on this hill receiving their undergraduate education. That said, we're also, this is also a moment where we're looking at, well, are there new opportunities for us to expand what Morehouse can do? I mean, in a matter of a week, we moved our entire offering, academic offering online. Or there's some ways we can capture that and expand our reach beyond the 64 acre campus of Morehouse College. But we're planning that there will continue to be 2,000 young men on this hill in the future once we get through this coronavirus moment. And the other experience that we've had is this has been a moment to really galvanize the Morehouse community. So for example, within less than a week, we raised over $200,000 to support Morehouse students. To help them evacuate the campus and place themselves in living situations where they could successfully engage Morehouse online. And we're gonna use this as a platform to increase support for Morehouse College. A lot is being made of the stimulus package that was recently signed by the president with a, you know, for coronavirus support. And about 1.5 billion of that is scheduled or targeted for historically black institutions. But there's been recent analysis that suggests that even this amount of money, you know, shared between our sector may not be sufficient to offset some of the losses that we'll have with enrollment drop in and costs associated with trying to get your campus disinfected and things resituated to just to get back to normal operation. Are you concerned about some of those issues? Are you concerned about what enrollment patterns may look like? Are you concerned about staffing and revenue building? And if some of those things will have to change even when we're back online, hopefully by this fall, is there gonna be a new way that we think about higher educations? There gonna be a way, a new way that we think about Morehouse operationally.  To not have graduated from Morehouse, that's high praise from you, brother. Okay, because at the end of many of your, you know, the way you phrased it at the end of many of your statements, the answer is yes, yes, yes. And like a good interviewer, the next unstated question is, and so. Right, right. Because, yeah. You know, people ask me what I'm most concerned about, and the reality is, you know, in the very short term, Morehouse has responded, and I should say the Morehouse community has responded extraordinarily well to the immediate crisis. We evacuated the campus without incident. Our faculty, alumni have all stepped up. All of our students we know are in safe places. Our online, we went online last week, and we're able to look at student attendance and, you know, classes are happening. If you ask me what I'm really worried about, it's about the fall. The country will go into recession, I believe. And I'm a, you know, 30 plus years as a business professor and my PhD is in business. I think we will go into recession during the last recession in 2008. Morehouse lost more than 25% of its enrollment by the time we were three years post-2008. And, you know, that's because given our distinctive mission, the families we serve, they're more vulnerable to these kinds of moments in our economy than the majority population. So I am worried about how the coming recession is gonna affect our student population. And, you know, I think we've done some things in anticipation of that, that will help us, help our students and our families. But I think for historically black colleges, like Morehouse, you know, this is a very critical period in terms of how the economy is gonna impact us. Good news, we've been working with legislators in Washington. There is a carve out in the stimulus bill for historically black colleges. At Morehouse, I've made it very clear to my folks, we don't only want the HBCU money, we want, you know, any money in that bill that we qualify for. And I think, you know, the good news there is as I've read the bill, I think it can be helpful to colleges like Morehouse. The final thing I would ask you, and this is more on a personal note, you would think with all the coverage and all the good you've been able to do and all the money you've raised and all the energy you've generated from inside the Morehouse community, you're still less than three years into this thing. I'm very much less than two years, I'm actually, I've been three years, I'm actually two years and three months minus three days. January one, right, that's it. January one. 18, I believe, January one, 18. That's right, January one, 18. And the things that you've had to deal with, I mean, just the presidential transition, rebuilding and building new trust and let's bring some of our old folks back together and let's put this thing back together in the way that people were used to have seen it. And now you got a pandemic. Do you look at your successes and failures and say, there are some lessons I can take from those things and apply them even in a moment like this that no one has ever seen before. And we're still in the midst of it. Do you think that there are things that you've learned at Morehouse that you've done right, that you've done wrong and you'll say, even with this big hill to climb yet, I think that even in my brief time, we'll be able to make some positive things happen still, even with something that we don't know what the end will look like. Oh yeah, yeah, yeah. Yeah, yeah, there's no question. One, the world needs Morehouse.  Right, you know, you think about where the world is today. The world needs Morehouse. The values of Morehouse, leadership, service, being transformational in challenging the status quo. And also the Morehouse idea, which is the expectation of blackmail excellence, that it's not an exception that blackmail or excellence it's an expectation. And part of the reason why people are drawn to Morehouse is most people have never been at a place where that's an expectation. And the world continues to need that. And men who come out of Morehouse and moments like this, you know, I think make that even more resonant. You know, the other things I've learned is the strength of the Morehouse community. I mean, if there are very few schools that within the course of seven days raised more than a quarter of a million dollars to support students who had to evacuate their campuses. And, you know, I'm talking about schools much bigger than Morehouse and much richer. And the reason we were able to do that, right, is because of, you know, when we galvanized the Morehouse community to support Morehouse, they defied gravity. And so one of the things that I'm thinking about is, you know, how do I grab that energy and use it to help us move to the future of Morehouse, because this is a moment that's shown that it's there. And, you know, the other lesson I've learned is our students are amazing. I've worked with our student leadership to help us make some of our hardest choices. You know, for example, about graduation and making sure that we're successful going online they've been amazing. So, you know, it's been a crisis, but for me it's been a very reinforcing and reassuring moment for all the reasons that I came to Morehouse to begin with two years ago.


This landmark study, a manipulating antioxidant intake in asthma, found that just a few extra servings of fruits and veggies a day can powerfully reduce asthma exacerbation rates. If it's the antioxidant doing it, why can't we instead just take some antioxidant pills? Because they don't seem to work. Studies using antioxidant supplements on respiratory or allergic diseases have mostly shown no beneficial effects. This discrepancy between studies relating to fruit and vegetable intake, compared with those using antioxidant supplements, may indicate the importance of the whole food rather than individual components. For example, in the Harvard Nurses Health study, women who got the most vitamin E from diet appeared to be at half the risk for asthma, which may help explain why nut consumption is associated with significantly lower rates of wheezing, but vitamin E supplements did not appear to help. Men who eat a lot of apples appear to have superior lung function, same with kids who eat fresh fruit every day, as measured by FEV1, which is basically how much air you can forcibly blow out in one second. The more fruit, salad, and green vegetables kids ate, the greater their lung function appeared. Why no data points for more than once daily consumption of salad and veggies? Because so few kids made the cut. They were cautious about concluding which nutrient might be responsible. Yes, there's vitamin C in all three, but there's lots of other antioxidants, for example, so-called vitamin P. Polyphenol phytonutrients found in grapes, and flax seeds, and beans, berries, broccoli, and apples, citrus, herbs, tea, soy. Turns out they can directly bind allergenic proteins and render them hypoallergenic to slip under our body's radar. And if that first line of defense fails, they can inhibit the activation of the allergic response and prevent the ensuing inflammation, and so may not only work for prevention, but for treatment as well. Mostly available evidence is weak, though, in terms of using supplements containing isolated phytonutrients to treat allergic diseases. You could just give people fruits and vegetables to eat, but then you can't do a double-blind study to see if they work better than placebo. So researchers decided to try to use pills containing plant food extracts. It's kind of a middle ground, better than isolated plant chemicals, but not as complete as whole foods, but you can stick them in a capsule for experimental purposes so you can pair them to sugar pill placebo capsules. The first trial involved giving people extracts of apple skins. I've talked about the big problem they have in Japan with seed or allergy, so apple extract pills were given every day for a few months, starting right before pollen season started. The results were pretty disappointing, maybe a little less sneezing, but didn't seem to help their stuffy noses or itchy eyes. What about a tomato extract? Randomized double-blind placebo-controlled 8-week trial for perennial allergic rhinitis. This time not for seasonal pollen, but for year-round allergies to things like dust mites. There's lots of drugs out there, but you may have to take them every day year-round, so how about some tomato pills instead? Significant improvement of total nasal symptoms scores, combined sneezing, running nose, and nasal obstruction were all observed after oral administration of tomato extract for 8 weeks with no apparent adverse effects. Would whole tomatoes work even better? If only researchers would design an experiment directly comparing phytonutrient supplements to actual fruits and vegetables, head-to-head against asthma, but such a study had never been done until now. Same amazing study that compared the seven fruit and vegetable a day diet to three fruits and vegetables, then commenced a parallel, randomized controlled supplementation trial with capsules of tomato extract, which boasts the power of five tomatoes and one little pill. And the study subjects were given three pills a day. So who did better? The group that ate seven servings of actual fruits and vegetables a day, or the group that ate three servings a day, but also took 15 supposed serving equivalents in pill form. The pills didn't help at all. Improvements in lung function and asthma control were evident only after increased fruit and vegetable intake, which suggests that the whole food interventions are most effective. Most of the supplements in increased fruit and vegetable intake were effective methods for increasing carotenoid concentrations in the bloodstream, but who cares? The clinical improvements, the getting better from disease, was evident only as a result of an increased produce intake, not pill intake. The results provide further evidence that whole food approaches should be used to achieve maximum efficacy of antioxidant interventions. And if this is what just a few more plants can do, what might a whole diet composed of plants accomplish? I'll cover that next.


Narcissists are boring, they are uninteresting and exciting, they are dull, tedious and repetitious, they are lacking in variety and interest. When you first meet the narcissist, you could be fooled into believing that you are around a fun and interesting person. They are very good at providing you with short bursts of amusement and enjoyment. But it never lasts long, they just tell you what you want to hear or show you what you want to see, but it's never what they're really about. Narcissists are not capable of engaging in fun activities for long periods of time, they can only create the illusion that they are fun and interesting. Their idea of fun is usually to take you to a movie, something that will distract you from their dull personalities. Because they already know that if you were to sit down with them with no distractions, you would quickly realize how dull and boring they really are. There would be nothing to talk about, you would have nothing in common with them, you would realize that they're not really about anything, they have no passions, interests or ambitions. There's nothing that makes them tick, there's nothing that drives them, there's no heart or soul and actually the reason why they targeted you is because they don't have any of that. They targeted you because they realized that you have everything that they are missing and they were hoping that you were provided to them, they were hoping that you would make their lives more fun and exciting. They thought that you would bring the pie to them, they thought that you would give them something to live for, they noticed that you had that energy, that spark, that sense of liveliness and excitement about you and they wanted to be a part of that. They wanted some of that in their lives. The problem is that when you get involved with a narcissist, although it may seem great at first, you soon realize that nothing can make them happy, nothing can make them have a good time, they always see things as being not good enough or beneath them, they always have to see something being wrong, they're always dissatisfied with something, they're never happy or content with anything, they always feel as though something is not as good as it should be and this has an effect on you because now you're around a downer, no matter how fun or exciting the activity may be. It's like you've taken a depressant or tranquilizing drug, you can't enjoy anything anymore because when someone is around you, constantly expressing their dissatisfaction for something, it affects your personal experience, you no longer get to perceive the world in the way that you normally would. You no longer get to enjoy activities that would otherwise be satisfying or fulfilling to you and you may find that you begin to lose your happiness, you begin to lose your energy, that spark which you once had, it begins to fizzle out until you feel dissatisfied, you feel like you can't enjoy anything, you may feel like when you're alone, your energy begins to regenerate, that spark begins to come back, but you never have enough time to come back to your old self because the narcissist is constantly around you, misery loves company, they are suffering but they are confident to know that you are also unhappy and that is why they constantly need to be around you because the thought of you being happy or having a good time without them is just too painful for them to deal with, in their minds they believe that if they have to be miserable then so should you, you shouldn't get to be happy, if they have to suffer that's how they see it, but as long as you are around a narcissist you will never find true happiness, you can try to dig deep within their souls but all you will find is more emptiness and it's just covered by things that appear fun or exciting, narcissists will often give the illusion of fun when you first meet them, they may act as though they are really interested in you and as though they have a lot going on in their lives, they will dress well and may even have a nice car, this all gives you the illusion that you are dealing with a fun and exciting person but if you just sat down with them for a few minutes and tried to find any real emotional purpose you would realise that there's just nothing there because these kinds of people only focus on the illusion, they focus on their image or reputation, it's designed to make you think that there's something there but it's just an empty shell, it's just nice packaging and once you've unwrapped a gift you're disappointed, it's not what you thought it would be because you were intentionally given a mistaken impression, they made you believe that they were these fun and exciting people just to lure you in, just to get your attention when they already knew that there was nothing fun about them, that's why they go through all the effort of creating the illusion just to make you believe that they're everything you could want them to be but when it all comes down to it, you realise it was just deception and now all you're left with is some crappy gift and some useless packaging. Thank you for watching, I hope this video resonates with you, please like, comment, share and subscribe, click the bell icon to receive notifications for my future videos, if you would like to donate my paypal link it's in the video description, coaching and queries you can email me at thanks for having coaching at duma.com, thank you for watching and I'll talk to you soon