How many times have you set a goal or your New Year's resolution and then completely forgotten about the New Year's resolution with a goal? Today we're going to talk about how to improve your goal hitting by using this one simple goal setting technique. And so there's a thing called Pearson's Law and that's what we're going to dive into today. Pearson's Law says this, when performance is measured, performance improves. When performance is measured and reported back, the rate of improvement accelerates. So what exactly does that mean? Well, if you want to track something that you're trying to get better at, you will improve. If you track something and report it back to somebody else, the rate that you're going to improve gets way better. And so let me give you an example of how you could use it and how I've used it before even with some of my clients in the past. About four years ago, I had a client that started working with me and the first thing he wanted to work on was he wanted to lose some weight. And in no form of anything am I a weight loss coach, I'm not a fitness coach, any of those things. But I knew Pearson's Law and so we used it to our advantage. So if he were to go and just jump on the scale every single morning and look at what he weighed if he was trying to lose weight, he would lose weight just because the fact that he would be measuring it. But if he has to report back to me every single week with his exact weight, he's going to improve exponentially. So this is what we did. I had him buy a scale that was connected to his phone through Bluetooth. In every single morning, he was required to get onto that scale, have his phone read it through Bluetooth, which automatically logs his body weight, his body fat percentage, his muscle mass, his water weight, all of those things. And then just you step on it within two seconds, it's automatically logged inside your phone. And then every week when we had our calls, he would have to send me the screenshot of every single weigh in from the week before, which kept him on track, but also gave him somebody to hold him accountable to what he knew he was supposed to do. And just this simple strategy improved his weight loss exponentially. So he weighed about 230 when we first started working together and he lost about 35 pounds within the first four months of us working together, simply because he was tracking every single day so he could see if he was improving or he could see if he was getting worse. And on the days that he got worse, it then made him go to the gym and work out harder because he knew that we were going to be talking every single week to improve it. And so once again, at its simplest form, if you track it, it will improve. If you track it and have to tell somebody your results weekly, you'll improve exponentially because now you have an accountability partner. And think about this. It's super important. Quantification in your life and in your business are critical to your success. If you think about it, no company just goes, you know what, we're going to just wing it this month. We're going to wing it this year and just hopefully we do well. No, they come down, they sit down and say, okay, we're going to sit down at their sales team. We're going to figure out exactly what it is that we need to do, how much we need to have them sell this month.  They have to track all of these things and they have to figure out their P&Ls. And that's exactly how your life should be the same way that you go into planning a business and making a business plan. You should go into planning your life and acting and being as serious as you would with a business on your own personal life. And so when we're working towards a goal, we have to track everything that we have going on. And we have to make sure that we have someone to hold us accountable to it. This could be how much money you want to make. This could be how much you want to save.  These are all things that can be tracked, measured, and then recorded and brought back to somebody else to hold you accountable. Because how often, think about this, just really honestly think about this for a second. How many times have you set a goal or your New Year's resolution and then completely forgotten about the New Year's resolution or the goal? Think about that.   How do I remember what they were? Think about how often you've set goals for yourself or set New Year's resolutions. And let's just take New Year's resolutions out of it. Let's take that completely out of it because that happens once a year. How many times have you set goals for yourself? A daily goal, a weekly goal, a monthly goal, a quarterly goal, and then nothing happens. You look back after your quarterly goal three months down the road and you're in the exact same position that you were before. Financially, mentally, physically, all of those things are exactly the same. So you have to take this Pearson's Law and say, okay, I need to get really good at quantifying my entire life. I need to get really good at quantifying my entire business. Why? Because numbers can be tracked. And if you can track them, you can record them. And if you can record them, you can give them to somebody else to hold you accountable to them. I bet 95% of the reason why people don't hit their goals, myself included in the past of this as well, is simply because they don't track them. They don't put them down and then they don't develop a system to hold themselves accountable throughout the entire year to hit this goal.   You know, if you say, I want to save $6,000 this year, amazing, perfect. Let's go ahead and take that goal and figure out how to make it measurable and how you can hold yourself accountable to it. So if you want to save $6,000 this year, that would be about $500 per month, about $125 per week, right? So for some of you might say $6,000, that's nothing to me. Some of you guys might say $6,000, that's the world to me. Okay, we'll just take this example as, you know, just simple proof. I want to save $6,000 this year. That's $500 a month.  I'm going to check in with you every single month, January 1st, February 1st, March, so on, so on, so on. I'm going to set a reoccurring event inside of my schedule that's going to link up to your schedule that's going to say, I have to call you at the beginning of every single month and tell you how much money I've saved. Can I get your, can I get your, you're holding me accountable to that. Is that okay? Your friend's like, yeah, of course you can do that. Cool. Perfect. I'm going to send you out the calendar right now. Just make sure you're accepted. It's going to be for every, the first of every single month this year.  You now have to call them because they're expecting your call because they literally have to hold you accountable because it's in their calendar as well.  John's going to go, if John's a good accountability partner, John's going to go, well, isn't that less than you told me you wanted to save per month? Yes, it is. Okay. So what are you going to do about it? Well, if I was supposed to get 500 and save $500 this month and I only saved 400, that means that this next month I need to save 600. So my goal for this month to hold myself accountable and stay on track is I need to hit 600. Okay. Great.  Boom. You're back on track. Perfect. That's what you want is someone that you know that you have to report back to in a quantity that you can go and say, listen, this is the number. This is what I need you to hold me accountable to. It's so simple, but it's so overlooked by most people. Most people usually don't even think about it. Hey, if you're enjoying this video, do me a favor and hit that like button down below. It helps with the YouTube algorithm so that more people can see this message because it helps us get it out organically. So hit that like button and I appreciate you. Same goes for health for your weight loss. Let's say you're like, okay, John, I want to lose 25 pounds this year. It's January 1st. I want to lose 25 pounds this year. That's about two pounds per month. I'm going to put something in your schedule. January, February, March, first for all of those.  So here's what we're going to do.  I weigh, you know, let's make the math easy. 225 pounds. I want to get to 200. That's two pounds per month. I have to be at 223 by the next time I call you.  I have to tell him what you weigh. You want to make it even better? Do exactly what I was saying. Get a Bluetooth scale.  And if you really want to have John hold you accountable, send him a screenshot of what you weigh every single week. And make sure that you check in. You say, John, I have to check. I have to weigh myself every single day. I want you to hold me accountable to that every single day.  You send him the screenshot every single week. He looks at it and he's like, hey, you only, you only weighed yourself five times last week. What happened? Oh yeah. And you make excuses. Excuses are like buttholes. Everybody's got them and they all stink. John, hopefully isn't going to take your excuses. He's going to go, hey, Rob, I don't care about what happened last week. You missed it twice. That wasn't your goal. You need to get it to me seven times, you know, check in seven times over this next week. How does that sound? Good. Can we agree to that? Boom. What's good about this is that if he holds you hardcore accountable to what your goals are, he probably also has goals that he wants you to hold them accountable to. So you create this accountability partnership where you guys are both trying to help each other hit your goals. So then if you come in and at the end of the month, you only lost one pound, maybe you gained a pound. Oh my gosh. He better be on you to fix that. That's for sure. And so that's how you create this accountability relationship. Do you have someone in your life that you can check in with every single week or check in with every single month? If not, you need to change your sphere of influence. You got to have at least one person that's in your corner that loves you unconditionally, that wants the best for you and has no ulterior motive to want to help you. They just want to see you thrive.  You need them. Even if it's just one, you need to have one of those people that's in your life. A couple of years ago, my girlfriend and I started sitting down with our New Year's resolutions and planning them out together and saying, hey, this is my goal for this year. I want you to hold me accountable to it.  Can you do that for me? Yes. And I need you to tell me this.  We don't get mad at each other. This doesn't turn into a fight. I'm going to come to you and say, hey, listen, I'm going to come to you with love and say, listen, this is what your goal was. You're short on your goal. We need to change this. What's your plan to change? And this is how you improve exponentially. Without weekly or monthly measurement, you can't make adjustments to your goals.  If you don't have that number, then you're not, I'm just going to be honest with you, you're not going to save $6,000 for the course of the year, most likely. But if you know, OK, I'm only at $400, and then you look at it and you say, I need to make some adjustments. What are the adjustments I need to make? OK, I need to stop eating out as much. I probably spent an extra $100 just eating out. OK, maybe I shouldn't have coffee in the mornings from Starbucks. Maybe I should make coffee because it cost me $0.25 versus $5. And you start making small incremental adjustments. Same thing with weight, same thing with working out. OK, I only worked out three times last week. I didn't hit my goal. Maybe I need to work out four times this week. OK, let me look at what I ate every single day last week. If I look back, oh my gosh, I probably shouldn't have that burgers and fries when I was out.  And you can make small incremental adjustments when you start to track everything that's going on. Because rarely do we set a goal, make a plan to hit that goal, and then just hit it our first try. There's a little adjustments that need to be had over the course of a month or a course of a year. And when you track all of these things, it makes it really easy to look back and go, ah, there's my mistake. I can point out my mistake right there. That day, that week, that action, that habit. And you can make small incremental adjustments just as if I'm in Austin, if I want to go to Houston, there might be a car accident at some point in time. My GPS is going to take me there, but if there's a car accident, I'm going to have ways. Ways is going to take me around that accident, hopefully, and get me there. Detours in your life are necessary on your road to hitting your goals. But if you're not tracking it, number one, if you don't know what the goal is, you know, actually know what the goal is and the quantity, the can't quantify it. It's going to be really hard to hit the goal. And if you don't track it, you're going to make adjustments. But when you know the goal, exactly, you're very clear on it. You track everything that you do. It's super simple to look back at how the last week went, how the last month went, and go, these are little tweaks that I need to make because we have to make detours. Things come up. That's just the way that it goes. You know, we're going to fall short some weeks. We're not going to be perfect all the time. That's part of being a human. But here's the thing, when you fall short, you can't get emotional about it. You've got to look at it as if it was a business and you go, okay, the business is not doing well in here, here, and here. What are the things that we need to improve?  I'm not going to put any emotion into it. I'm going to say, here are the things that I need to improve on. What adjustments do I need to make?   We're going to do this together. We're going to do this before the end of the year. We're going to do this over the course of the whole next year. We're going to hold each other accountable, even if you've never talked about goals before. Who's that person in your life? If you don't know who they are, identify them. If you don't have someone, find someone. These are the types of people that you need in your life. If you look around and you go, none of these people would ever hold me accountable to my goals and they'd probably make fun of me if I shared my goals with them, maybe they're not the best person for your life. And I'm just saying it, just maybe you should spend less time with them. Spend time with people who are supporting you. What I want you to do is think this, when you're thinking about these people to hold you accountable. If you look at them, are they someone, like the place that they're in in their life, do you want to be in their position at some time in the future? Ask yourself that. Whether they're a year ahead, 5 years ahead, 10 years ahead, do you want to be in their place in the future? Or if you look at them in where they want to go, not where they say they want to go, but where they're actually working at going, do you see them and go, yeah, this person's on the same track as me? That's the thing that I can say. I've picked up mentors along the way that are further ahead. But by far, the one thing that's helped me out the most is since 2006, I've had a best friend. We talk almost every single day about our goals. He's my business partner. We've built multi-million dollar businesses together and we've grown together over the past 14 years. I know that if neither one of us had met each other, we would be way further behind in our lives than us meeting each other and being able to work together. 14 years we've been working diligently, diligently, diligently. I mean, he's been on the podcast. His name is Dean. You guys have heard him before. We've literally talked, we've talked four times today alone.  They're on your side, no matter what.  If you don't, it's okay, not a big deal. But now you know who you need to search for because you need someone to not only track this stuff, you're going to track it, but you need to go back and actually tell them what it is. So identify that person, find that person and bring them into your life because once again, Pearson's Law says when performance is measured, performance improved. When performance is measured and reported back, the rate of improvement accelerates. The rate of improvement accelerates. Hey, thanks so much for watching this video. If you want to learn even more about Master Your Mind, click right here and watch this video as well. Today we're going to be talking about the six steps to hit any goal you want. Coming up.


še hom občine z med enfinernosti molekularne norespečne simulati. Petr je, da smo videli ideju tem molekularne norespečne simulati, vzifelja na kuheljega, še, da smo vsak bunkot vso občini vse, in so se museli vzwišti numerikali. Tudi sem je veliko guriku, in izgledajte to v nekaj timestep, a danes ideje in prinsipelje za molekularne modeli. Načaj početimo, če je tukaj nekaj aspekt, če je molekularna dinamica? Simulacija. Zato zelo smo videli tukaj timestep, tukaj kondition, in taj držav od tempratu in priču v prvom početku. Zelo, da se pričeš, da je simulacija konditijna. In v drugim početku zelo, da se zelo, kako zelo, zelo, in na konfiguracijenem početku. Zelo, taj početek. Taj početek predaj, kako je taj početek sem simulati. tako, kako je komputacija, je to zelo stejnje, zato zelo stejnje in zelo stejnje in zelo stejnje. Tako, je to zelo stejnje. Na različenem vse možemo simulati zelo stejnje. Tako, vzelo stejnje je zelo stejnje. Zelo stejnje je zelo stejnje. Zelo stejnje je zelo stejnje. Tako, vse zelo stejnje je schovil, in zelo stejnje. Tako, jen 딱 se tem počok je skupnago, in tizje dober je, če je jazel se izgleda, zelo stejnje je zelo stejnje. je složan smelice. But when it's too small, nothing happens and it takes too long before a cure and that's not what usually we want, since we have always the problem that we don't simulate long enough. If it's too large, it causes instability and then we cannot cure immediately, inитivno, kot vsopnike je z vzključenjo zvedanje. Pa v verti, when you choose a molecular model, you have to pay attention to, how this molecular model is set and how the time step is set for this molecular model. Not all the force field for example is the same, it was the same time step. When we have an appropriate time step we can describe izgleden kompromis in simulacija tudi v izgledenih v nostrih sistem. In otroj aspekt, zelo način in atomistih, način in atomistih skupnji, kjer smo vzostajili bombe, uspežimo dve femtosekom tudi. in je div juice dveč, da tudi je zemlječna mojda, s kajim sedaj trenenčem potenšeljem sa vstupném dramatizacijem. Tležito prej dveče je kot srečo relacionira s potenšeljem in z dvečem potenšeljem, da je začalač nerečna potenšelja. Vse nekaj umel Naj nekičnijo starčče nerečno potenšče, in kar da je šaljel potenšel, da je šaljel način. Tudi je to zelo, zato, da kako najmaj glasbeni model da je šaljel potenšel, danes automistik model, so vsega izgledaj šaljel način. In da se izgleda, da se izgleda, da je šaljel način between the molecular model that you use, and the time step that you have to use with that molecular model. There are different ways that can be used to increase the time step. And one I was mentioned already is to apply constraint to your bond. There are different constrain algorithm. Example are links, parallel links, shake. And usually the time step is correlated to the fast motion, and the fast motion is correlated to low mass. So for example, it's very frequent to put constrain on the bond involving hydrogen atoms. Another way is also to use virtual side change. So also here, if we look on the fastest motion, this will involve the hydrogen, but not only the vibration of the bond, but also the angle and the torsion. In particular, for example, for the material group of the amine group. So the use of this virtual interaction side allowed us to remove this fast motion and in this way to increase the time step. How it works? So for example, here you see in gold the position of the virtual side. And so the force is calculated on the atoms of your system, then is redistributed to the virtual side, then the Newton equation is integrated, and then from the virtual side we reconstruct the position of the original atoms. There are also other approach that can be used to increase the time step. One is also to play on the mass of the atom, to make light atom more heavy. But in this way we affect the dynamics also of the system. Then we go more in detail in the simulation condition. So usually we want to simulate, we simulate one molecule, but we want, and this molecule is mimic a molecule that is together with more molecule in, for example, in a covert. So if we look at the baker of an experiment, there are very few molecules that are in contact with the border of the baker. And that is the same that we would like in our simulation, even if we simulate one molecule. And for this way, we implement a boundary condition, and most the simulation are run in boundary condition. What, how this work? So we have a simulation box that is in red, that is our simulation box. Then this simulation box is replicated in all the direction x, y and z. That means that is surround by around 26 other box that are just image of the original box. That means that when one particle is going out on this side of the box, it's just coming in on the other side. This is, so, and this is how we do to avoid to have boundary in our system. We have to define our box. We usually put our molecule inside the box. And the standard idea is that this box is cubic over a tangular. But as we saw before, we have a problem in calculating all the non-bonded interaction, particularly the solvent. So the calculation of the non-bonded interaction, if you think your box is full of water, is computational avie and make our simulations lower. So one option is to try to build the box in a way that is optimized the volume around the macromolec. Usually the macromolec has a global shape, so probably a spherical description is more suitable. So we have been developed a different shape of molecule, of box that are try, that are optimize the volume. For example, for main brain simulation, exagonal box can be a good option since we just have the 85% of the volume of a cubic corresponding cubic box. For a standard global protein, maybe one can think about truncated odecaedric box and that is approximately 77% of the cube or better, orthorobic odecaedric box. That is almost spherical, looks like a sphere. That is resemble the 71% of a cubic box. In this case, since in the edge, as you can see in the of the box, you mainly have only solvent, make everything around will make us save interaction that will save time. So we have to calculate less interaction, non bonded interaction, without losing description of our system. That is also what it is. Another condition that we can define is the thermodynamic ensemble in which we want to run. There are different thermodynamic ensemble. There is MV ensemble, so constant number of particle, constant volume and constant energy, total energy. That is an ensemble that you use mainly in the physical simulation, but not so much in bio-physical and biochemistry. Bio-physical and biochemistry experimented on and then as a consequence simulation, are mainly in perform and canonical ensemble like constant number of particle, constant volume, constant temperature or at the constant number of particle, constant pressure, constant temperature, what is called MPT ensemble. So these are the two conditions where mainly experiment are run. And as a consequence, since simulation, usually are running parallel with the experiment or they have to compensate the lack of information of the experiment, we also are interested to simulate in this condition. That require that we need some approach how to couple the temperature and the pressure of our system. And so now we will see how we can calculate temperature and pressure in MD, what is the way in which we can couple the temperature and the pressure. So the temperature in the molecular dynamic simulation is calculated from the kinetic energy. So the kinetic energy is given by the mass of each particle, the velocity square of each particle over two. And then for the co-partition theorem, we know that this is equal to the number of degree of freedom of the system. And the number of degree of freedom of the system is equal to the number of particle of the system, multiply by three minus the number of cost strain of the system. Then we have kB, it is the constant, Boltzmann constant, and then the temperature divided by two. So if we know the kinetic energy, we know the degree of freedom, we know the Boltzmann constant, we can extract the temperature of that system. So the temperature of my system can be some way controlled by modifying the velocity of the particle. If I modify the velocity, I have a different temperature. So usually the algorithm that we use to control the temperature, we call it thermostat. And the role of the thermostat is to provide us to ensure that the correct average temperature of the system and that the coefficient of the temperature are of the correct size. There are several thermostats, so one of the original thermostat, that was the oldest one, developed in 1991, is the balance with coupling. Thermostat is very efficient in relaxing a system to the target temperature, so the relaxation of the temperature is going in an exponential way. But it does not reproduce the correct fluctuation of the kinetic energy and as a consequence of the temperature. So then it doesn't resemble a good canonical ensemble. In 2007, Bussim developed another algorithm, Velocity rescale temperature coupling, that is an algorithm that is based on balance in thermostat, but has an additional stochastic term. And these ensure a correct distribution of the kinetic energy. So now we have a thermostat that provides not only the correct average temperature, but also a correct fluctuation of the temperature. Then we have another thermostat that provides also a correct canonical ensemble and that is Nose-Uver temperature coupling. This is the philosophy behind this algorithm is different, thermostat is different. Here a thermal reservoir and a friction term is added to the system Hamiltonian. That is peculiarity of this thermostat is that it is relaxed in an oscillatory way, the temperature. So if we are very far away from the target temperature, that might be a problem. So it's not used in an equilibration phase. Then we go to see how we calculate the pressure in molecular dynamics simulation. The pressure is related to the volume of the system and to the interaction between the particle. So if we can see, it's calculated from a first part where we have a degree of freedom, Boltzmann constant temperature divide over the volume and this part is resemble us the kinetic energy. So actually is twice the kinetic energy and then we divide for the volume v is the volume from our box. Then we have a second term and this second term is the virial that describe the contribution due to the force between the particle. And this can be easy calculated in MD when we calculated the force in the phase where we calculated. From this we can extract that the pressure can be controlled by varying the volume of the system and scaling the position of the atoms, of the molecules. So when we couple the pressure, we speak about barostat. We have different way to couple the pressure. We can couple it in an isotropic way. So it means that we couple in the same way along x, y, and z. Or we can couple in same isotropic way. So we couple in the same way in x and y, mind in a different way in z. Or we can couple in a completely an isotropic way. There is also an option that to couple not the pressure but at the surface tension. So here again I go through some example of barostat and as also was in the case for the barostat before is I don't cover all the barostat, all the thermostat. So here also not the oldest but one of the oldest thermostat barostat is the Berenzin and that scale the coordinate and the box factor every step. And that has also the same problem that is reproducing correct the average pressure but it provide a wrong fluctuation of the value of the pressure. So an alternative, a good alternative that was coming out very recent in 2020 Habernetti and Pussy is stochastic cell rescaling. This algorithm is very similar to the Berenzin algorithm but a stochastic term is added and that allow the good reproduction of the fluctuation. Then another alternative is called Paterinello Rama and these thermostat work somehow like nozehover temporal coupling, so I had the term to the Hamiltonian. And also in this case the relaxation of the pressure is go in an oscillatory way. That my cause is that the system is very far away from from the reference pressure, strange oscillatory behavior in the box. So now we go further with more so we saw the pressure and the temperature. How they calculate and we go further with that information on how the system set in. So we saw the condition, so it's a boundary condition, how the temperature, the pressure. And now we go to see how we set the system. Molecule are always in a condensate phase and they are in an environment that is or the cellular environment or an environment that try to mimic the cellular environment to be so close possible to the cellular environment. So that is also what we would like in the simulation. So when we are simulating a membrane protein we set it in a membrane, we would like that the composition of the membrane, the lipid composition of the membrane resemble as much as possible the lipid composition of the original membrane. We will set this, the protein in solution and usually we want to have ionic strands so close, so possible to the experimental one. If it's a protein for example in seawater where we have a 0.6 ionic strands, 0.6 molar ionic strands, we want to have the same type of ionic strands. So we usually add to our macromolec to a solvent, ion, another type of molecule that are present in the experiment. Another, then we enter is another problem when we set the system that we need the macromolec or the molecule that we are interested in. So we need to have original coordinate. This can come from an experimental structure that can be an x-ray structure and an MR structure, cryo-M structure. Or it can also be a structure that is built using for example, homodulin modeling, docking approach, also so some other geometrical criteria, for example, that are usually used, for example, to build DNA or name molecule. For solvent, we also need a starting structure for the solvent. So usually for the solvent we need to build a solvent box, relax, equilibrate. And then we can use this solvent box to solvate our macromolec. These, there is, there are database where we can find that this is one of the database, the protein data bank, where we can download PDB structure. So structure of file with the position of all the atoms of our macromolec. And that we can use as a starting point, for example, and then is what we will do in the tutorial later. There are, can be several type of issue. So not always when we have a deposit structure in the protein data bank, all the position of all the atoms are solved. Some atom might be mixing or some atoms might be just model. So that means that behind experimental structure there is already a simulation going on, but we don't know. So one has always to check the experimental structure and usually to understand which are the really the raw data that come from the experiment and which are the part that has been model to make better the refinement, for example. We sometimes happens that a very key flexible loop maybe is missing. So we have to think about how we want implemented. We can use software like Modeler to model it. And then we have also to think about to relax. Also another issue with experimental structure may be the position of the hydrogen. Hydrogen are not always available experimentally. And all they might be available, but they are specific of the condition of that experiment. And that might not be exactly the condition that you have in your simulation. So that has always to think about. And for example, a PK shift can occur that this might be frequent in the pocket of a box. So it might be the protonation states of the race in that pocket might be affected by the present or not of the ligand. It could be that different atomics states occur and automerism is very difficult to detect with almost in experimental technique. The only technique that provide us some information that automerism is NMR. Then in X-ray structure in mainly in X-ray structure we might have the position of other things like water for example, but it's very hard to know if those are water or armenizium ion because it's almost very difficult to distinguish or sometimes the criteria used to distinguish them is just based on the fact if one can form or not hydrogen bond. But the question are what relevant are those water? Are just you there to the crystallization procedure or are key water that play a role in the function of the molecule? Also in experimental structure to get the structure we might have other extra molecule co-factor, ligand, sulfatane or they can be performed in a special condition that has to promote to see the experimental structure but not necessary will correspond to your condition. So when you take a structure you have to think all of this and also when you interpret your results you have to have an overview of this. And then we have our structure. We are happy with our structure so it means that we have the position of the atom in a file. And probably when we want to start the simulation our system will also have a temperature. So if we start like this it means that we start at temperature equal zero. This is one option. Or the other option is to assign random in the velocity such a way that the distribution of this velocity reproduce the desire temperature. One way for example is that the velocity are taken from the random Maxwell distribution with a kinetic energy corresponding to the design temperature since we know that there is a relation between the kinetic energy and the temperature. So now we have seen in this second part the time step. We have seen the condition, simulation condition with boundary condition and the temperature pressure. Then we have seen how we set our starting structure, the ion, the problem of the selection of the starting structure. Now in this last part of my presentation I want just to speak about molecular simulation connected specific with Gromax that is the software that we will use in this summer school. As I told you, so when we start from our structure that it can be model or take from a database so we have already a priori choose how we want to describe the degree of freedom. For example, here we already decided to describe it in an atomistic way. Then we have downloaded our structure. Probably is a good procedure to visualize it to check if we miss something, if that is looks fine according to what we know. We probably have had already all the missing atom. Then we have to choose the force field to describe it. Since we are atomistic, we will choose one of the force field. We also have to define solvent in the ion so the environment that will be. Then we have in case we have some strange component that are not already present in the force field. We have probably to generate a mean implemented missing parameter and this should be in line with force field definition. And then we have to define in which condition, which temperature pressure we want to run, how long we want to run. And then we have this our starting simulation, starting structure that we can start to simulate. How this is described in term of Gromax so we can see which are the topology file that we have when we use Gromax. We have a specific, we have file that describe the structure, file that describe the simulation parameter and file that describe the topology of the system. So usually when you get the structure from the database for a PDB database, you can run a tools that provide you if this structure is one of the standard macromolecule, it will provide you a topology file. You have to option to choose which protonation states to have some control on the tatoominic states, to the protonation states, to the terminic and other small things. And this, so in this way you have generated your topology file. Then you have this, you have still your structure, but your structure probably is not in solution and you want to get to your structure as a structure in solution. So you will probably have to put your structure in a box. You have to solvate it, you have to add the ion. So to put the correct environment in your box, all the molecule that are required to describe the environment should be in the box. And this we will enter up in a structural file that can be in a growth format, in a PDB format, but they contain the coordinate of all the atoms of your system. Then you will proceed to have to define a file that is called MDP file, Simulation Parameter File, where we have all the setting of our simulation, how long we will simulate, which condition pressure and temperature, for example, boundary condition, all the setting of our simulation. And now we are almost ready. So we have our structure file, we have our simulation parameter file, we have a file that describe the topology of our system. Then we need the tools that put all this information together and you create a new file that contain all the input information. Then this file will be processed by tools called MD Run, and it will provide a series of output, a trajectory where according to the criteria that we have saved all different time point of our system, a file that contains all energy information and a log file. So this is how we perform a simulation, how we have to set the simulation. Usually when we start from the beginning, we have different step in this simulation. Usually when we start from a molecule from the database, we have a first step where we energy minimize, so we relax the molecule in the potential that we have used to describe the model. So we have a first phase of energy minimization. Then we have also a second phase where we will relax the position of the solvent and lion around the macromolecule. Then we will clearly break the whole system and we will move to a data production. And the data production will provide us an ensemble of conformation, from which we can extract a different type of property that can be thermodynamic property, structural property, kinetic project, dynamic property, mechanical property. So we can extract different type of property. We can see that in each of these steps, before going to the data production, we use a different type of NDP parameter. When you finish your simulation, and then you are performing analysis, one other thing that always I say, and then I think it's very important, is to visualize your trajectory. There are different visualization tools. Here I list some of them, but it's very important that you visualize your trajectory because by looking what happens to the atom, you might discover things different at what you thought you want to analyze. It might also help you to set and calibrate better your analysis. With this, so after visualization of our molecule, I thank you for your attention. And I see you in the Q&A section where I hope to get a lot of questions. Any curiosity, anything that you didn't understand completely, please ask. In the future, after this summer school, if you still have question on particular gromax, please go to the forum and ask or look, maybe someone else ask already what you have, you are interested in, and there is already the answer. Thank you very much.


If there was if we had to figure out I'm glad we got to do it above the battle Are you clicking buttons again? How's it going everybody? Dato do I hear back with some more Dragon Ball fighters ranked matches and this time we are finishing off Broly week with the OG say in trio you got Dragon Ball Super Broly base Goku and base Vegeta This was actually a highly requested team for the week I think mostly because one it's just a cool theme team, but two you can combo Broly's level one into spirit bomb You already know we're gonna be trying that today's overhead. No, he lands behind didn't even go for a lot I was just I was slow on the draw on that one. I kind of want to switch out if I'm being honest I'm gonna switch out to Vegeta. Oh He did two ki blasts. I'm a Super Dash. I held it down They block that we don't want to see that overhead can't get anything off of it So it's not the worst Broly you want to talk to this man. I Figured you'd have a few choice words. Haha. I figured there'd be a few for choice words I'm gonna go sliding just because we're so high that I don't think we'll be able to give me this man Yeah, I wanted to see if that work. Oh Just in general Broly and base Goku are a really good pair. I don't know how much of that I'm gonna be able to show off I cuz my base Goku isn't the hottest, but They they do make a great duo Which is nice because there's a lot of base Goku players now switch. It was obvious, but he didn't punish with the 2h Dragon Rush. Oh, that was poorly spaced No, let me get that counter Broly Where was the man base Goku? All right, we'll live this we'll live this and I think at this point We should probably be looking to switch again Just keep that assist alive. I could have come with that That let's go big hit on this one. All right.  I just didn't get to come out Yeah, we need go home Am I gonna live to regret this? I hope not I'm not gonna I'm not regretting anything Let me calm off this. I regret I regret so much We can still reason. What are you doing clicking buttons base Goku? Ha ha ha he really clicked super-dash Face Goku didn't want to see that Well, that was an expensive way to kill somebody but you know when you when you gotta spend it. Oh It's all come together now boys. I'm gonna go for the solo No, and then yeah, I could have gone with that But I was so happy that it actually worked that I have I lost the plot Ha ha ha fate spirit bomb into the medium shoulder. Oh Some of these some of these tactics you don't learn at base Goku school, dude All right, I'm gonna do the whole thing One two three. I needed to go sideways From the top of the Thunderdome. Oh, come on. What are you doing? Oh? No, no, don't bring me back up.  Ah, I forgot all right Well, I'm glad we tried it just for fun then If there was if we had to figure out I'm glad we got to do it above the battle Are you clicking buttons again?  Broly doesn't like seeing people click buttons. Just just jump back and hold block Jump back and hold block. That's oh, I was talking trash.  I'm expecting a super-dash. Oh, no, I wasted spark for that. Oh my goodness Big drop. Oh base Vegeta with the absolute clutch save. Oh, no, I got hit. I can block this. I Can block all this come on. Oh so fast and then the six frame sweater weather that oh that was too fast Trunks actually has a lot of tools in sparking Behind the back word. Oh, I'm gonna lose the whole game I'm gonna lose the whole game off that one. Oh No, um, if I lose this just remember me for the man. I was not for the man. I became Get opened up anybody else sweating or is it just me? Oh No, I Don't I wonder when that mic went up. Oh No, dude, I Couldn't have seen it coming. It was beyond me to see something that fast dude Armor, baby We got armor for days Loop it. We keep the loops a little fresh here. We keep them fresh All right, number 15. That's goku down Broly just decided he was gonna show up. Oh, come on Hey, do you want to jump? Look maybe maybe too much of a read on that one Is this free? No, that's not free There's still so many things I haven't been in situations where I've gotten the chance to try on that finish off that Beam assist, I mean I even beam assist just the beam out of the assist is all you really need to get going double tag I like that option get that beam assist back out here. It's gonna be a goku be goku extravaganza All right, do I have an extra jump? Let me test it. All right. We do have an extra jump Let me charge the spear bomb All right All right, we got the beam assist down And uh, we're in relatively good health So i'm feeling rather good The only reason i'm doing this is because base vegeta base vegeta is the only one not necessary to the plan Ace vegeta can you know, he can do whatever he wants, but he doesn't have to be here Oh Oh, wow base vegeta. Okay. We can take out trunks. Oh that might have been the okay. Okay. He rolled out He rolled out. He rolled out and base vegeta should live this as well All right, looks like we are going to be just fine here. Maybe even a potential switch Okay Please hit Yes, nice Okay, so we have vegeta back out now Is that what we want? I think so Okay I'll respect your turn. That was a bad reflect. Please don't judge Okay, it should be perfect. Oh got you back in the corner. All right. We don't need this character out though Oh, I don't know if I want this character out either. Okay. I need I need a little more space Again, I did it the wrong way. It's so complicated I don't know where broly ends up. I really should have practiced this That's fine. I think we're gonna go with the old classic mix up Is he going to grab or keep last? Ah, well the mix up It's not the most potent mix up Okay, hold on. Hold on. Hold on. Hold on. Are we down? Take care probably into buttons It would have worked All right base vegeta. Thank the w that's a that's a big win for somebody I was talking a lot of trash of in the middle of that game, but You know, it's all coming together broly did it exceptionally well that match I could have gotten an early uh Kill or knockout as well. Uh, but unfortunately for broly. I decided to go Damn, I wanted the don't go there button was was nice, but it just didn't work out We spark early. I'm a spark Ah, too. I shouldn't have used the jab there 2l 2l is a much better button in that case Broly no I were good. We're good. We're good. We're good. Switch. I need an emergency tag He should live this Scaling beyond our side Oh, oh, no We're gonna lose broly. Oh, you can't tag unless you get some beam assist Oh, no I tried not to go into super dash, but in the reality was that move still too ageable We're we're getting momentum the momentum is definitely not on our side early spark We didn't get the momentum to turn around. So now we're just we're really dumbfounded now All right flash there Backdash Again I shouldn't have done this Okay, I'm glad we finally got a hit if nothing else. He didn't jump after that. Get me over the beam We actually lived because of that Let's go. No my turn. Oh, he He should have killed me there, but he just didn't go for it My turn. Ah, I thought he wouldn't mash. I thought he would I thought he would try to get a little fancy He'd get a desperate for the kill trying over it. Ah, damn. Okay. Hold on. I can still make this come back with just the base poo Oh, maybe not. Oh, give me that. Oh, give me that It is a scramble kind of game He's it has been the 24 seven scramble though. I can't say I blame him We're cooking. Why is base Goku doing the most right now? Okay, I'm gonna do the two bars This gives hard knockdown as well Nice I think he thought I would fake it. All right. That's why that was my main reason for not faking it Back it up. Don't put me back here. Hey, nice back dash. Oh, I thought that was kind of You're gonna tell me that wasn't pretty even don't mash. He did dp That was even that was even smarter than mashing. I do that. Oh Dp no dp. Oh, I try to I try to go button for button with him unless he drops it. It's pretty much over Ha ha Oh, give me that really no punish Oh, I'm dead. I over committed. I didn't have enough health to commit like that. Why did I I was so afraid I think I I was so focused on his dp And I was trying to bait it out with every button But at that help you really have to consider vanish and I just I wasn't thinking of it two one two one Let me readjust in the seat here. We need a that that was a momentum changer Oh again, I try to get him in that air to air I'm trying to win those battles This is a very bad situation to be in for a broly Let me out. Let me out. Let me out All right, Vegeta. You trying to take this away. I got that extra jump. Yes, siri Go in there, Vegeta do work Imagine if I got hit there I can't block that for the life of me. I wanted to keep him in the corner, but oh that uppercut assist just decking me Just blow blow Vegeta away. Oh, all right. I guess I understand Really? I'm I'm not getting these continuations like I want. He's just reflecting me off Uh spear pump stride is looking less and less like I was looking for the reflect. I don't know if he even went for it there Okay to Okay Get broly back in there. Get broly back in we need two bars for spirit bomb I don't know if we're gonna get that or not, but I want broly in there at least. Oh, I panicked Oh, no get the base crew Get the base crew assist in there Doesn't have to be all bad Okay Okay, uh, I think we have to switch out of Vegeta to continue this I'm trying to look out for those reflects. I just I just keep clicking buttons.  Get off me zone zone zone Zone can't believe I misplaced those buttons. No, dude. It's getting so why is this game feels so intense? Oh poor Vegeta Poor Vegeta He did his best out there, man Look at the blue health he was putting on gohan if we just got to follow through it's up to the rest of us now Come on, dude Okay, that's that's perfect. That's perfect. Show me gohan Show it to me. Come here gohan Yep, I can never that's not how that move works. Oh falling down into the beam Surprise that works, but I'll take it. All right, bro.   What is happening? It's only two two. Is it? I feel it. Well, it's not two two yet, but That's assuming I actually win this game Then we can make it two two time, dude.   Let's go. I'm blocking. I'm not I'm not gonna repeat the same mistake twice I gotta play a little bit safer. All right, two two And we're heading into the final game if there was ever a time to pull off that spirit bomb It would be right now It would be now And I think because of that, I'm gonna let the g to do the talking. Wow. He still beat me in the air to air By the way No matter what I do. I cannot beat this man air to air. It just does not happen. All right, hold on I feel like looking up The calm I know it's not difficult, but Okay Getting opened up by a little bit of stagger pressure. I see I could kill him right here. So I'm going to I'm sorry, but when the opportunity presents itself. I gotta take it. I got I gotta prioritize the win overall But we are going back out into Vegeta almost immediately. Oh Finally No super dash I'm gonna make super dash look real good as an option right now Oh, no, why did I switch out to goku? He pressed the button. We're back in the green Hold on. Hold on. Hold on one two three. Let's go Let's go. Don't get caught Oh, I told you not to get caught pushing buttons fall I told you not to get caught pushing buttons, bro Is that dead? That's not that but it's crazy how he jumps after this He never jumps dear. He never does this man does not jump after knockdown is It is giving us those lags more and more frequently as unfortunately, that is All right. Well, this one feels a little obvious. I gotta get trunks back out here Oh right back down into two age zone Welcome back, man Okay, hold on hold on Did we just make out like a bandit dude? I feel like we just got out like kings That was pretty tricky though. I did a key a key charging can be seen as disrespectful But I feel like if that's the strap, bro, go for it did not do the right super No, what are you doing here vegeta? Oh okay Oh, no, dude, please Just allow somebody else to come in vegeta All right.  That'd be pretty sick Oh, he's going level seven He called it. No, he got he's got the level seven damn it Invincible If I can't spirit bob you I've got a love got a level one super you dude and it'll be invincible No, no, bro. We do it. I can't believe I can't believe it registered as a super dash Oh, that's so disappointing. Don't lose don't lose me. Oh, no I'm really working on a sweet loss right now.  No Oh my god, okay Okay, the base a a team is risky Something about this team makes me a little extra cocky, bro I don't know why I wanted to hit the spirit bomb that bad But regardless, hopefully you all enjoyed sorry. We couldn't hit the spirit bomb today, but I might try again I might try again in the future Let me know what your favorite dragon ball super broly team is down below in the comments And while you're down there make sure to the like button and subscribe if you enjoy this channel and the videos We make here also make sure to check out the other videos on your screen right now if any of them catcher I I have been dr. Doia and I will see you in the next video


What determines the buying power of the currency? How does it stabilize and what's required to stabilize? So if I would buy some bitcoins, who can manipulate the value of that? Everyone The buying power of Bitcoin is determined in exactly the same way that the buying power of the euro the British sterling the Japanese yen Or the US dollar is determined through market forces of supply and demand in international liquid markets that operate around the clock One of the fundamental differences is that Bitcoin trading never ceases has been going continuously for seven years The network never stops every ten minutes bitcoins hard beats and Transactions are processed the exchanges never closed there is no closing price for Bitcoin It is a rolling average and in that trading a Market capitalization of approximately 12 billion dollars is now traded internationally What is 12 billion dollars for a global currency? It's a guppy swimming in shark infested waters and Every trader every whale goes in there and just kicks that price around So right now the experience of living on Bitcoin, which I have been doing full-time for more than three years Is a roller coaster? It's an absolute roller coaster I've seen shifts of 20 or 30 percent in a day and yet if you look at the long-term trend volume goes up transactions go up and Volatility keeps dropping and the beauty of it is I can't sell that to an American. I can't sell that to a Brit. I Don't need to sell it to an Argentinian. I don't need to sell it to a Brazilian I don't need to sell it to a Venezuelan. I Went to a conference and an Argentinian told me I'm not worried about volatility our currency has volatility like this Bitcoin has volatility like this I Rather be going in that direction and you don't need to tell them why Their government threw people out of airplanes not more than 35 years ago for disagreeing They already know why the separation of state and money is a good idea and so Volatility is relative


one of the things that's been really useful on the mountain is having a private toilet every company does differently but for our company we had a private toilet for the whole team which is eight team members so i want to give you a walkthrough and show you how this works so this is the the toilet tent it's usually closed at night and then if you want to use it you just end it all the way up and welcome to the toilet so this is uh this is the toilet seat you wouldn't expect that on the mountain but in every camp we would have the exact same toilet i have to do open the toilet seat this is uh you know this button right here you just push it all the way go ahead in and out like so just to kind of block it and keep it kind of tidy and then you don't want anything splashing out yeah so yeah so when you're using the toilet you want to make sure that it's opened all the way and then this is the flushing system all you have to do just pump up and down and then you would dispense some water so you flush boom boom if it's clean it's good and then they have their scrubby scrubby do thing usually want to lift the lid before you do that obviously clean and then once you clean it you just close this and then boom shakalaka and for the men in here if you needed to you can pick up on the sides right so you can get both up and that's good manners for the rest of us may i ask that you point something else out too toilet paper oh sure go ahead uh they have toilet paper for us already that's not a problem i think the first few days we didn't really but we bring ours anyways so that's one thing there are actually throughout the hike at many camps there are toilets they're not the best but public toilets that everyone can use one thing that alex was telling me earlier is that if you don't have a company doesn't have a private toilet like this you cannot use the the camp kosovo camp which is one of the camps that requires a special permit for the company to have in order for the team to spend the night there so by way of having this we were able to spend the night at kosovo camp so that's the toilet situation and we're gonna leave right now close it all the way and then obviously good manners if you want to make sure that no one is there knock knock is there anyone there no i don't need to use it that's it