Welcome back after the break, we just looked at chapter 2, we'll continue with chapter 3, if anyone has any more questions? Any more questions? Yes, Francis, can you please give the mic to Francis? I don't know, it's not even the Bible. What's the name of Samuel's Bible? No, I don't think it's mentioned in the Bible. Yes. Like, he's got will, is okay for broken marriages. Is it God's will for broken marriages? Because like, I have heard and seen of people who are in ministry, but still they're marriage are broken up. What do you think, is it God's will for broken marriages? No way. But like, why come God, like, why come it happens? We can't attribute everything that happens to God, sometimes it's our own choice that we make, we make the wrong choice and we can't attribute it to God. So sometimes we see that this is a good person, the person is rich, my person is earning well, well qualified, I'm in ministry, the person can listen. But you know, then the person is coming and is worldly and not able to get into the ministry mode or have that heart for God. But when two persons were in ministry, like I know about one minister, she's a songwriter, she's a singer and her husband is also in ministry. They ministry in same foundation, but still they got married and then they broke up. Many people were in ministry, that's what being in ministry doesn't make us automatically angels, doesn't mean everything is going to be perfect. We are still going to be human. We are more in the forefront of the battle where we need to guard ourselves, guard our lives, our ministry, our personal lives and that is why we are studying this book. We importance for that. Yes, good questions. Thank you. Yes, Anand. When coming to the ministry, you have told about the priorities that man and woman write. I don't know, can I ask this question or not? It's okay. No problem, you ask it. People used to tell like women can't preach, don't preach in pulpit and the things. There are some priorities to man and woman that God has given. And in the same way that we see Hezra's in our ministries also, right? Hezra's. Hezra's. Hezra's. No, Hezra's not. Hezra, you mean Hezra, the scribe? Transgenders, okay. Transgenders. I saw so many big, big pastors telling like transgenders don't do ministry and preach. So they are not the persons who is in a position to preach. Can they also preach word of God being as a pastor? What do you think transgenders? Did God make transgenders? Did God create transgenders? I only know He created Adam and Eve. He did not create... He created Adam to be like Adam, Adam race. He created Eve and Eve race. He did not create... He did not create somebody to be a blend of both, right? Somebody to move to the sun. So He did not create transgenders. It's just the Bible says that it's just, you know, that is a lie of the enemy that they have believed. And that's not the truth. So can they preach? I don't think they should preach because they're basically living a lifestyle that is a lie. So what truth they can preach? One person that I know, the person who got transgender, so he got baptized and came to know that Jesus Christ. And so after that, he started a ministry also in between the transgenders to preach the word. Then it's okay because he was somebody who was living that lifestyle. But then he repented of his sins, he's baptized and then he can go back and he can minister of that. Why not? When he's living his true identity of who he is in Christ and what God who God has created him to be, then he can go and minister. Yes. He set himself right with Christ. Is it okay? Some professors comparing the lives before the transgender and after the transgender that they can't preach the word of God. No, no. Anyone who is in Christ, they are called a royal priesthood. They are ministers of God. Yes. And can women preach, you said? Can women preach and teach? Oh this. Okay. Okay. Shiv Kumar says, I think they can. Who do you think can? Shiv Kumar? Can you just qualify your statement? No, I need to make sure that's what. Shiv Kumar, who can teach? He says, I think they can. So who's the day? Can you qualify please? Yes. It's a question about life partner, ma'am. Question about life partner. Life partner. Yeah. Like I saw many pastors, they used to say like particular one person for one girl or one boy. So is this like God created only one person for one boy or God created boy and girl. It's my choice to choose someone, choose someone for me. I'm getting my question right? Yes. God created from heaven me, right? And the same time for my partner. Only one partner is there or it's my choice to choose my partner. He has a specific plan and purpose for your life. He's going to show you which career, which field you want to join, the job, all of those things. The same way he has someone for you. And yes, he will, if you are asking him, he will show you, he will guide you. Actually like that's my choice, right? Whom I'll get married. Yes. And your choice will be, if your will is aligned to God's will, then you will also align, it's a good question, it's a very important question. You will also align your will to God's will in the area of marriage as well. And you will know from the inner witness, the Holy Spirit, your conscience where God is leading you. Yes, this is the right person. And God will also show you through the inner witness, the Holy Spirit, the peace, everything goes well. It's the right person. Yeah, because I'm asking this question because there's many people die before like when they are kids, right?  So if God created a particular one person for them, it's not like that. No, it's not. Yeah, that's my question. No, no, it's not. Transcenders who accept Christ, yes, yes. Thank you, Shiv Kumar. Nina says, Nina says, within what God has instituted for the proper functioning for whom it is also the role of the husband to love Christ, to love the Christ love, in which case submitting is not a burden. The word also says submit to one another. Yes, Nina's question. Submit the word of reverence for Christ. We submit because we're submitting to Christ just like she said, yes. Thank you, Nina, John for adding that. Yes. Next, she has a mic. Then pass it on to Sean, please. Okay, quick, quick, quick, pass it on. Sean, come here, Sean, because the mic is here. You can quickly come. Ma'am, I'm just sick. I want to ask, what if...  Which people? Gay people. Homosexuals, okay?  Okay. So what do you make of that? I mean, God has strictly said in the Bible that no man is supposed to love another man or a woman or another woman, but down there becoming pastors, what does that mean? It means that how perverted they are becoming in the word of God. And what does Paul write in Romans chapter 1, he says, God says, I will give them up. I will let them go their own ways. Their choice, I will let them go to their own choice. That's why their minds are perverted. This is talking about the perversion of the mind. So the minds are perverted because they are not worshipping me. They are worshipping created things. Even though I have made myself visible through nature, the invisible attributes of God, the eternal power of God, his deities, made visible in creation.   So God does not treat us like puppets on strings. He gives us our own gift of volition and choice. So what do I make of it?  We just have to pay for them that God will open their eyes to see the truth. Yes. You have a question? We posted. So ma'am, is marriage is also predestined by God? Oh, no, no, nothing is predestined by God. The predestined to be conformed to the likeness of his image and son. We are predestined to be conformed to his glory. But God does not predestine our choices that we are going to make. He knows the choices before knows the choices that we are going to make, but he does not predestine. If I say God predestines our choices. That means we're saying is a partial God. He predestined some to go to heaven.  He's not a partial God. He knows for those two choices that we're going to make, but he does not predestine our choices. the choice is us but does he know he does know but what is the predestination he predestines us to be conformed to the image of his likeness of the Sun predestined to be conformed to his glory a predestined to be like him to be his all that after we know him as God and Savior so he does not predestined who we're going to marry but he knows who will marry what the choice is yeah he knows who we are going to marry he knows somebody who can you know son but that person can say I don't want to marry you see so that's your own choice that person's own choice that doesn't mean that God is going to run out of choices he will lead us to another person yes Rin I've heard of other pastors like God really into the womb like in a congregation like a person she married that person they both are supposed to be together and so is it I mean the process is that God told me but to the people of the believers it's something that that they disagree that you know pastor saying that we got told me you both have to get married what did we learn about when we hear Godly council everything that we said all of those you know guideposts list that we talked about accepting for the word of God we have to validate everything by going back to scripture right yeah in receiving God's guidance remember those nine things that we've eight things I think seven or eight things you know stirring within receiving council knowing within all that has to be validated with scripture so the pastor says God is telling me you both need to get married then you both go and pray and ask God if God has revealed it to the pastor he will reveal it to you as well but just don't do it because the pastor says but you need to pray and hear from God as well so sometimes a pastor can hear wrong sometimes it can be even pastors are just doing it to manipulate people to keep the people in their own you know all the manipulative techniques that some of the pastors use just keep the sheep within them to control over them to exercise their Lordship we look at it okay but if the pastor says very genuinely you pray and if it's God's will you know he will guide both of you and give you the peace yes Sean ma'am like God has everyone has God has made a set plan of everyone right now suppose if a particular person like deviates from the plan and does God's plan change apparently or is that also part of God's plan that he might deviate and then come back again like what do you think from all that we learned in this last uh August September October you answer me that question so God has a plan for you and you go away from God's plan you think God changes his plan to adjust to your plans no okay thank God yes he does not adjust his plan still fold okay does he wait for you to come back does he stir you up to come back yes when you come back does he take you back and on the line for your path and for answer purposes so you answer your question thank you yes one last question given the mic please i think the class is waking up good i think that is also just like God reveals his plan and purpose to your life he will reveal this as well yes so you just need to ask pray and wait on God and he will repeat yes any more questions yes Francis what's Francis question how what what how do you know your spouse before marriage you pray and ask God and ask you to show you God to show you yes there's some people i know that you know God has told them specifically this is where you're going to meet your spouse this is how he's going to look at like and it has happened exactly God is interested in analyzing every little thing yes Sean sorry the inner inner witness the Holy Spirit yes why not all of you very excited about marriage okay they must just be praying because you're a young person and they might just generally be praying but if you just want a confirmation you can ask it from God and you know you can ask God to give you like a word of prophecy show you specifically yes yes Sean yeah why not you can meet your life partner 50 or 60 years well if you are going to delay things things will get delayed for you yeah but delays from God are not denials but we know delays comes not because of God but because of us so yes Francis this question is God has a spouse for me and i don't want to marry her what will she do you don't have to worry because God will bring a better spouse for her so God has many spouses for her a better one who will God will bring okay so good he's worried about the spouse okay okay let's move on any more questions okay we move on to lesson three okay now kristin ministry sadly people think it's about an organization it's about a church it's about big church events programs mission trips writing books producing songs you know building buildings planting more churches well all of this is part of christian ministry but basically christian ministry is all about people okay christian ministry is all about people it's about ministering to people is reaching out to people it's about touching lives with God's power to rescuing them from the whole of satan bringing them into God's marvelous light you know nurturing them into christ likeness and you know seeing them be released into the full destiny plan and purpose that God has for their lives and in that process you know we include all of these things you know worship song writing books you know building buildings you know doing various events organized you know crusades and all of that is basically to build people it's not just to build our business or our ministry okay so ministry basically about people is having them encounter Jesus Christ seeing their lives transformed into the image of Jesus Christ okay so even as ministry is all about people we also know that people are the biggest challenge right uh ministering to people dealing with people working with people is not easy it's going to be very very difficult you know we need to understand how to treat people well how to lead them how to manage people how to correct them how to equip them empower them and release them into their destiny so this is something that we need to learn because people are important uh and also ministering to people is the biggest challenge because people can be difficult okay we'll just look at a few lessons that we can learn from the life of Paul you know in 1 Corinthians chapter 3 verse 9 Paul says tells the people you are God's field you are God's building so who is this you is referring to the believers the church at Corinth so he's telling them you know the the people are God's field the people are God's building um so uh you know ministry is all about serving people and it's all about building people we need to get that right okay um you know and we hear to lift people up we see here to transform them into the likeness of Jesus Christ get them to see their divine destiny their calling and their purpose another thing we can look from learn from Paul's life in 2 Corinthians chapter 3 verse 2 Paul says you are our epistle written in our hearts so where are people written you know in Paul's hearts that means he valued people he treasured them who they were his prized possession okay and it's important to value people it's important to have people in our hearts when we have people in our hearts that's when we will not look for fame and for money and for power and to misuse people you know to have to boss over them because we are looking at people as somebody who are you know who are valuable to us our price possession they're here to serve them we're here to give into their lives we're here to speak over their lives so everything that you do you will have a heart for people you will reach out to people you will invest into their lives another thing we can learn what Paul writes is in 1st Thessalonians chapter 2 verses 19 and 20 he says you know when he stands before God what is his joy his hope and his reward he says you you people who I have served who I ministered were brought to the Lord so he's saying it's not the mission trips I went the number of churches I established the number of leaders I trained the number of young people I've trained the number of churches that I've established the number of episodes that I have written no it is you you are our hope our joy our crown our reward when we stand before God it's you that we are going to be boasting about so when you write people in our hearts that's when God possess the freedom the power and the liberty to speak into their hearts see if we are here as ministers to make use of people I want to be nice to all these people I want to speak nicely to them I want to preach sermons that they want to listen to make them feel good so that they don't go up to some other churches that they give me more money they give all their tithes they give all their sun you know so that I can have a good lifestyle then we are actually misusing people we're not then God does not give us the freedom and the liberty to write into their hearts and such churches are churches where you know that we don't see the move of God we don't see the life of God we don't see people's lives being transformed but when we write people in our hearts it's also we see the Holy Spirit begins to transform work and move in a mighty way okay so when they're ministering to people we need to realize that people are in different stages of their Christian growth okay Paul is writing to the church at Corinth and he says you know there's some of them are babes some of them you know they even though they're you know they're flowing in the night in the gifts of the Spirit and all of that but still when it comes to the word of God they are still babes they're children they cannot be pet solid okay so we need to be careful we need to understand where people are we need to nurture them build them up in the ways of God look at what John chapter 16 verses 1 4 and 12 say you know Jesus also had people written in his heart and in his mind right you know one day when Jesus and his disciples were very tired he says let's take the boat and go to a quiet place and rest so they go to in the boat and they go to the other side what do they see already people have heard the news that Jesus is going to come to that side of the shore they all come running and the multitude of people are just waiting there and when Jesus sees that he says oh no no we want to get away from these people and rest and these people don't seem to be leaving us does he say that no the Bible says when he saw that he was moved with compassion and what he teaches and he teaches them and it becomes evening and the disciples come and say send them away you know others we have to get food for them Jesus says you give them food too and he said where can we go and get for so many people and then Jesus we know multiply the five robes and two fishes why did we do that because he had people written in his heart and that is why we can see the Holy Spirit moving so powerfully so look at what Jesus says you know he says in John chapter 16 these things I have spoken to you that you should not be made to stumble but these things I have told you that when the time comes you remember that I told you of them and these things I did not say to you at the beginning because I was with you I still have many things to say to you but you cannot bear them now so we see that you know Jesus was very purposeful in the sermons that he preached you know he he taught them things that they needed to know at those times some things that they did not need at that time he did not you know shared with them he knew that they will receive revelations later on the same goes with us you know when we are preaching we need to be mindful of you know our audience where they are what does they need you can ask God he will show you and then you can pray your you can preach the appropriate message okay don't preach what your audience wants to hear some some people want to hear on missions some people want to hear on evangelism some people want them you're on discipleship but as a pastor there's a leader you need to hear from the Holy Spirit you know prepare sermons topics throughout the year knowing where your congregation is knowing where they are and seeing how God wants you to lead them and guide them and they're not only for adults but also youth also with children you know where where in the children's ministry we're writing our own children's search curriculum all the topics that you're learning in bible college we're teaching our children's church we have written curriculum for various topics but you know we also sometimes we tell that and see okay where our children now you know after pandemic we realize all our children have grown and now they're gone to teen church those children were not part of the curriculum before have now joined so we shouldn't continue from where we left we need to go back and start from the very beginning so we need to see and understand and discern and do you know see where our audience is what is their needs and preach and teach likewise honor okay next one is honor everyone okay roman stwell says you know be kind affectionate give in honor give preference to one another okay matri chapter 10 jesus says word gives one of these little ones a cup of cold water in my name surely i say by no means you will lose the reward okay so don't treat people only the rich people they educated the high class well speak them nicely treat them well and don't you know overlook people who are insignificant small you know lower economic status of society not well educated but treat everyone equally and you know care genuinely for people you know people can see through when you're somebody who just pretends to hello praise the lord brother sister and you know all that people can see through you know people can see through when you're genuine and when you're just faking it so genuinely care for people okay the bible word of god teaches us that we need to honor our elders those who are in authority those who teach god's word are double honor look at what first timothy chapter five verse seven says in your books we'll have the the recent publication i have the old one so that is why you're we are on different pages okay so you know first timothy chapter five verse seven paul is writing to timothy he says all those who are rulers who elders especially those who teach the word they receive what what should i receive double honor okay and then in first the salonians five he says esteem very highly in love those who are over you in the law that means we respect everyone who are preaching teaching you know some of your pastors you go back you know they don't know anything about computers they have not gone to bible college they don't know about gifts of spirit uh speaking in tongues and all of those things you don't look down on them you know you don't treat them as they are you know they lost it and all that you still give them the honor the double honor because they are people who are teaching and preaching god's word okay show no partiality paul is writing to young timothy in first timothy chapter five he says you know show no partiality and he says you know when uh to show pa you know don't show partiality he says you know who are your witnesses look at that verse who are your witnesses says the lord jesus and the elect angels are your witnesses so even if people are not watching even if i'm not there to come to episcenal timothy know this as christ and the angels who are watching over you so do not uh you know uh be partial and also in uh james chapter two um you know um it says that in the last verse in verse nine if you show partiality you commit sin sin is not only lying and cheating and murdering and using bad words and adultery sin is also showing partiality okay so don't commit that sin don't show partiality um treat everyone equally even when it comes to discipline if you have to discipline somebody in your church who is well educated or very rich you might think oh if i discipline this person he might not come to church you might leave my church i will lose all the money um you know and all of that you know don't bother just discipline um in in a godly way in the right way in a loving way okay be grateful say thank you okay thank you does not make you feel small uh thank anyone and everyone whether it's maids people are the gatekeeper you know driver whoever you know people who are serving just thank them um because if it's not for them things would not go smoothly and you know it says in god's word that each one of us even though we are individual members we have different functions in the previous price and each one is used up for the edification for the building up of the church okay if somebody shares things with you in confidence please maintain confident confidentiality don't go and you know uh share it with your wife with your children some things you need to just keep it personal don't share it with the church staff or the elders in the church uh don't also use it as an example you know sometimes we give an example uh and we explain the scenario because you have to explain it to give that example and people in the church can identify they're always thinking who's this person who's this person who's this person then they will be trying and the person who's listening to it they will feel very very not broken and disappointed so avoid using examples avoid using examples of your wife your children uh in your messages because you know they will feel that you're letting them down if you want to use some examples get that permission before you you see example and i've heard pastors say i've asked my wife i can i've asked my children if i can use this example they give me the permission so i'm saying it so that means people are saying okay when i go and share with this pastor he's not going to share my secrets out so he's an reliable person to share things with okay correct people lovingly you know first Corinthians chapter 16 verse 14 says let all that you do do in be done in love okay uh you know uh correct people with a good heart with a good intention with a good attitude uh two things pastor says when he corrects people is first he warns them so he calls them he hears from them he tries to let them see what they're doing wrong uh he tries to explain things to them uh he shows them how they can what the steps they need to take to correct it so just the warning stage and then he gives them time you know to to make those corrections to heed the corrections that he has given them okay then if they are not going to you know uh heed that warning then he is going to bring in the then the second approach you know then he brings in the correction okay the correction also he says there's a cause and there's an effect some corrections are because the person basically doesn't have the skill not able to manage that time uh is not able to uh because they have lack of training in that area then pastor is coming in stepping in and he's bringing the corrective measures okay you take some time off get this training you know have this time management plan all of these things he can help but at some times when people you know um are lazy they are unwilling to work hard there is rebellion there is pride uh they have conflict with people you know bad attitudes they have selfish agendas they think they're very superstitious you know very high-minded uh at those times pastor's correction is very very simple okay because you can help people with time management you can help them if they don't let them lack the skills and all that but these attitude problems uh is is is going to be difficult because this is going to actually affect the ministry is going to affect uh people in the ministry it's setting a bad example to other people it's going to cause a lot of first drive division and all of that so to safeguard everything the best thing he does is send these people away or release them from their responsibility because basically you can't change an attitude of a person you can help them in their skills their training time management but not with their attitudes and because this cause their cause of rebellion their cause of conflict with people bad attitudes high-mindedness selfish agendas super spirituality is going to affect other people okay in those cases what pastor does is he releases that people or he sends them away he he takes away the responsibility okay but he says when he corrects people he does you know the right motive the right mind so that you know he has his conscience is not picking against god uh you know he's not doing anything that god does not want him to do okay um but this is all about attitudes of people but when you have a personal it's not talking about personal uh you know relationship attitudes uh you know personal issues that you have with people then you have to you know talk out your personal conflicts with the person try to resolve it if the person is not willing you can't change that person's will right you just let them go you just pray for them but you do what is right okay uh you always correct in private okay don't even when you correct in private don't make it known to others hey i send this person away because this person this is this you know uh this person is not right he's not a good leader he did this so i do move in from leadership position uh you can just say you know this person is no longer going to be a leader this other person is going to be the leader don't give the reasons just keep the reasons just to save god the person just to show that person hey we love you what you're doing is wrong but we still love you we don't want to put you down uh so the person will feel you know uh the freedom to still come back to church still be part of fellowship uh still serve christ but if you correct in public you know what's going to happen it's going to hurt that person that person can even leave church uh leave even the family of god have nothing to do with christ and become a rebel and you know things have worked out in the negative okay uh and when you applaud people applaud them in public uh just don't take names just say uh you know want to thank everybody everyone who did you know work hard for this crusade for this event for this program thank you everyone let's give them a big clap okay so just applaud everyone in um public okay some simple things that we can keep in mind um you know um have a personal strategy for handling difficult situations most of the time pastor says when he is um uh when he wants to correct somebody if it's his minor issue and little things he just sends an email okay uh if it's something little more uh which can be spoken of he does it over the phone but when it's a major issue difficult issue some things that he needs to really speak get an understanding because has a meeting with the person calls a person for a meeting meets hears that person he keeps on listening listening listening while he's listening to the person he's also listening to what god is telling him and then he communicates a decision to the person why not communicate you know decisions that you make um through email important decisions through email and phone because sometimes when they're writing people with their emotions can read your email with their emotions okay and then that can become you know can become very tragic so if you want to just correct somebody just very simple you can just say you know i think you could do this in this way this would be nice and just give you a suggestion fine some important things important to call the person and speak to them i've also learned from my mistakes sometimes i've just sent email and the person has read it uh i sent it in a group but the person has taken it very personally has read it with their own emotions the email i sent or my whatsapp message and it has happened uh it has a negative uh impact so better to talk personally face to face so that the person is able to see your reaction your heart attitude what you're trying to say your tone of voice and are able to understand you okay don't be a boss over god's people god's word clearly tells us that you know um in first peter chapter five versus one and four peter says shepherd the flock of god serving as overseers not with compulsion but willingly not for dishonest gain but eagerly and not being long over those interested to you okay so don't lord over the people of god be examples be a shepherd by setting an example okay many times uh you know shepherds have misused people uh you know they have abused people uh to serve their own purposes and it's very sad to hear what you know pastors do you know some pastors they will walk in front they will have one man holding their bible or their briefcase coming behind them uh you know they're going up to the pulpit or to the podium they have the man carrying their bible or briefcase i'm sure you have your two hands you can carry your bible or your briefcase once i was very shocked to see in one church in our own city um uh you know man a man of god uh his son wanted to go to the restroom and so there was another man following him he's a grown-up boy not even a girl was a grown-up boy but like a bodyguard you know man's running behind him so very sad thing to see so this is basically misusing uh people they're not to misuse people they're not to lord over their lives we're here to set an godly example uh and to build them up in christ likeness and serve people in christ likeness that's what christ is right he did not come to be served but to serve all of you with me okay last point do not control do not manipulate people uh sometimes you know um you know when people go to other bible studies they go to other crusades other meetings held by other pastors the pastor comes to know he he just sends out threats from the pulpit you know um and he um he starts saying how you know how he's so spiritual how he's so anointed he uses different testimonies and different things just show how anointed and how powerful he is compared to the other men of god in the city sometimes he can use a pulpit even to say you know in that church they're speaking wrong doctrines teaching wrong doctrines so don't go there um you know um but as as a as a minister of god we need to give people freedom you know if they want to go to another bible study group and they're learning from there let them go if they want to go to another prayer group and they're learning how to pray and they're being built up let them go you know if they're going to another church where they're having a an event where they've organized on some specific topic on the bible or something related to life and ministry let them go don't stop them let them learn from other ministers and men and women of um um of god uh don't be worried whether they'll come back to your church now if they belong to your church you know if they are your family they will come back home right even if your children or your spouse if you have a fight with your spouse you go away to office and uh in the night do you go and stay away somewhere else no you come back to your family even though you don't talk to each other don't look at each other you come back home you might have shouted at your children but your children don't tell the school teacher i do i love you more than my mommy i'm not going to go back to school i'm not going to go back home i want to stay here in the school no we don't do that so you know um people you know you feed them you train them you shepherd them you know they will always come back where they belong they will always come back where their family is so don't threaten people don't manipulate them don't control them yes anand as a spiritual father you protect all of your sheep from wrong teaching by just telling them from the word of god what is right what is wrong don't tell them that man of god that church that pastor this son we don't put down anyone if you hear anyone in the city who's teaching something wrong don't specifically tell them you know in that church you know this is all the wrong teaching people are very curious they'll go there and listen you know so you know then finally they will you know it's so deceptive they can easily get attached there so what you need to do is you need to be very smart you teach those wrong doctrines from the word of god teach them what is the truth so that when they are faced with the wrong doctrines you they know what they have been taught and they will make a choice themselves okay yes shan's question and then we'll stop because you're already running out of time okay can we take your question next week shan okay we'll take your question thank you everyone for joining class i'll see you next week thank you


The web of 2006 was a roller coaster to work with. jQuery was made to even out the lumpy platform the web offered and made cross-browser development easier. It did such a great job that it was not only widely adopted by developers, but even browser vendors were convinced by the usefulness of these APIs and they started shipping some of them natively. Let's look at an example. Arguably the most well-known feature of jQuery was the ability to find elements with a CSS selector. Here we are looking for a div that has the username class on it. This functionality proved to be so useful that browsers brought it to the platform under the name of query selector and query selector all. You can use query selector in all browsers, including into the Explorer 8. And here's a little fun fact, because so many developers are used to jQuery's dollar notation, it is even supported by DevTools. Neat. As you can see, jQuery had a lot of influence over the shape of the web. And we're gonna talk some more about that in the future episodes. So if you're interested, you should really subscribe to this channel. But in the meantime, why not watch some older Supercharged with this button?


what's up everyone take down here welcome back to another video today i'm going to be sharing with you some possible future wwe superstar releases let's get right into this so we see from time to time wwe releasing their superstars and the main reason they make any releases from their contracts is due to that superstar not doing so well in the ring and they have no direction for that superstar by releasing that superstar they make room for having call-ups or having new talent come to the wwe so i have a small list of possible wwe releases in the future that i can honestly see happen now not everybody on this list i believe should be released i believe they some of them should get an actual push because they are great in the ring i just see wwe never pushing them and never giving them a chance ever again up first on this list is heath slater he was originally part of the nexus group where he was a three-time wwe tag team champion he was part of the three-man band he was part of the social outcast along with other groups but none of them really took off until he started to team up with rino which i absolutely was a fan of and they ended up winning the smackdown tag team championships for the first time after rino left the company slater went back into single competition which he started to really become a jobber in my opinion he ended up winning the 24 seven championship but hasn't done much since next up is ec3 he returned to nxt in 2018 and in 2019 he made his debut on the main roster while he was in nxt he never won a championship he had great matches and great rivalries he just didn't have any championship success whenever he made his in-ring debut on the main roster he just didn't have that much potential in my opinion they weren't really pushing him and they've seen him not do too much in the ring they had kind of corny cutscenes and skits for him but nothing more than that he ended up winning the 24 seven championship four times but is currently out on injury due to a concussion so we don't know when he's going to return but unless wwe is going to push him whenever he returns i could see him getting released from the company or asking for his release due to the wwe not pushing him anymore next up is going to be no way jose which in my opinion is currently a jobber with the company he debuted in nxt in 2015 and made it to the main roster in 2018 i believe the only reason he made it to the main roster and stayed with the company as long as he did is due to him having a cool gimmick that people absolutely love which is the high fiesta dancing gimmick i think it is pretty cool it is an interesting gimmick except he hasn't had no success with the company he's never had a title hasn't had that great of matches in my opinion and i just don't see him going anywhere with the company and from time and time again we see this kind of gimmick getting released from the company and this kind of gimmick always seems to disappear down the line next is going to be titus o'neill which i think wwe should push him because he is a bigger guy and i feel like he could have the success if he was pushed in the right direction he was a wwe tag team champion a while back but hasn't done too much since he ended up winning the wwe 24-7 championship but other than that has not had the success in the wwe that i believe he should have i'm a huge fan of his i think he's great in the ring and he could have great matches and great rivalries but wwe just doesn't seem to be pushing him next up is going to be epico and primo they started wrestling from 2011 to 2013 then they started wrestling under the name of the loze matadors from 2013 to 2015 then the shining stars from 2016 to 2017 whenever they made the return to the company and then the colognes from 2017 to the present the only time they won the tag team championship in the company was in 2012 other than that they haven't really been a dominant for since and they've been really going downhill i feel like because wwe is not having them get pushed or having them even on tv anymore that i feel like they're going to be leaving the company very soon if not i think they already asked for their release next up is going to be apollo cruz he made his debut in nxt from 2014 to 2016 after that in 2016 he made his way to the main roster while he was in nxt he did have great matches and had great potential he just never won a championship and in 2016 when he came up to the main roster he's had great matches he's been aligned with the right people in my opinion he just hasn't had the success he's never won a championship and i just don't see wwe pushing him much further than this and the last name that i have on this list is shorty g aka chad gable he was in nxt from 2015 to 2016 and then made his way to the main roster he is a one-time nxt tag team champion and a one-time smackdown tag team champion both times with jason jordan and he was a raw tag team champion with robert rude when he was with jason jordan i could see the team having success in the wwe but after they split and now that jason jordan's in-ring career is in debate right now i just don't see them ever being a tag team once again since then chad gable was put in different tag teams with shelton benchman and then eventually robert rude but right now is in solo competition in the ring he is currently signed with smackdown where he is having odd matches against big guys i just don't see him getting pushed too much because he is a cruiserweight the name shorty g to me just does not make sense they should have left it as chad gable that was way more appealing to most people and i just don't see him having success in the wwe unless he was getting pushed into the cruiserweight division i feel if he was part of 205 live he would definitely start to take off so this is my list of future wwe releases comment down below any that you feel like i have missed now once again this doesn't mean that anybody on this list where people that i feel do not have the in-ring skills i'm a huge fan of a lot of these guys in the ring i just see that wwe is not pushing them and i could see wwe releasing them very soon so i hope you guys enjoyed this video please take care peace


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Get out of the braking. Yeah, as Alex said, we've loaded the brakes up and we haven't got the steel yet. Good. Monday? Yeah. Well, it's not good yet, but it's Monday morning, we're back here. Yeah, it's 9-11 today. We're back here, so as you had the update last time, I stripped all that down, Dad finished the back of the extension part and I've just moved it now.  I know. But this time, what's happening is, this part is getting, there's a steel going in and then there's going to be a pipe going up like around the back. But from the end, it looks like it's a 90 to all that. This just seems to be... I don't know if you can see. That wood's doing nothing. I don't know if the one behind it is, but that one, I ain't doing much. You could probably just, if that wasn't attached there, that'd just fall out. Oh my God. It's filming? Yeah. There you go. It's just punching into the wall and then God knows what's gone on there. It's like shrunk and it's moved. I'd leave that in there, in case there's brickwork drops. I don't know. It's floating at the moment, but if it does drop, that's not there at all. It does decide to drop. At least that'll stop it going all the way. Does that answer our question with the lint wall in the garage, though? Yeah, we should be okay. We've got to go up high in that anyway. We'll just chop the top off this little, leave this in. Because the garage doors attach these bits of 3B2.  This is nothing. This is just a stud. And that downspot goes down for gully in there. It's like a half-ass garage job, isn't it? It's like a corrugated concrete roof. So we'll do about the air, that.  Because it needs redoing anyway, doesn't it? I don't know where that's supposed to go now. Don't take any more off that. That's staining. I thought we were building over it? Yeah, over the top of it. I thought we were building the pillar? No, no pillar. We're punching just the wall that they've done. We're doing that with our steel. Right. But higher up. So what we need to do is get our scaffold set up, get this brickwork knocked off. At least we know we can take that down, take this out, and just take the top off this one mantle, and we can go above with our, because we need to build this up, this up and get our steel on top. We've got a small steel with a lip on it, for a nine-inch wall. So that single we're going to build is a double, solid wall, nine-inch. We're going more towards the lintelite of the house. So we are staying now. So what we'll do is we'll get the scaffold set up and start taking this brickwork down, chuck it on that big pillar. Since this house has been altered so many times, and badly, then we've got the laser out, and we're just using as pure as the data. That's two inches of steel, and it's levelled into the house, so the porch keeps it safe as a house. So we've won our garage doors with a minimum of the same height as that lintel. We spin it to the back. Our lintel is lower at the back, because it's the small door that was made to measure for the whole. The camera works at 100. So this lintel is 1560 above the laser. I'm pretty sure this goes at least two courses higher. They are on. Be as review-filled for me, please. So the back lintel is 1560 above the laser. The laser doesn't matter where it is, it's just a data line to measure. So this lintel is 1600. So that's only 40mm higher than that in there. It's a bit weird. But anyway, I think what we'll go for is that height, the amazing one higher. So the lintel's a simple height match. That gives a six, a seven foot two-garage door for a seven foot quite a decent sized door. So we've got all the lintels there. But we've got a steel to go in there. And the steel is where the roof, the steel might need chopping off on the end of the lintel. You know what I mean? Because it's got the upside of the steel. It's all hard to work out, but we just have to put it in and if it needs to be cut, we'll have to cut it off in situ when we come back and piece up the pikes. Because all we can do is put the pikes up completely off. And not when any cuts on, all the cuts are set right back so that when the roof's done we can just come on and piece it all up and set the knocking stuff off. It's got a weird pillar that sticks out. So this under here isn't part of the garage. That's the part of the garage door. So we'll have to build it round otherwise it'll get strange. Again, there's no drawings or anything, so it's on us. I can't believe that.  A big bush. So I think we've got a bit of a plan now. So we'll get this scaffold up. I think we're going to work to this lintel. Level through with that. So we don't have to touch the timber lintels inside. We can just go straight over the top of them. Which is good. Keeps everything secure. Makes it easy for us less demolishing to do. So yeah, Alex is going to start bringing this scaffold up. I'm going to get my boots on before it drops on my foot. And we're going to get this knocked down. Go back to town, that's where we get up. Nice, straight, flat, four planks. I wasn't expecting those. You see that? The reason why that's not falling. There is now each. That'll be sat on that timber lintel on the inside. So we can't leave it over that. That's why it's not falling over. There's some of the bush tree that was growing over the whole place.  This way you shouldn't have stuff growing around your house. Get that off there. Get this moved. At least chop it off. So we can get in here with our steel and our brick work. Which is going up to the... That one. That's our height. So that's top of our brick work. Down to basically mirroring that. Which looks a bit ropey at the moment. Like I said, we can't finish it until we get a roof to pitch it to. The roof is sitting on top of that. And then top two courses for the flashing. So the roof comes off flashing up to the window. All the way across. Yeah.  Not all of it, just so we can get this wall down. Obviously no flashing there. Down onto the roof. Luckily we don't have to mess around with that roof. It's corrugated concrete. But it could be asbestos. So luckily we don't have to touch it. And it's all very damp as well. So there'll be no dust coming off that today. So once Alex gets back, we'll get some tools up and start knocking this down. Make some sense of this so we can get ourselves organised. Put it back together again. I'll have to see if we can get the steel at one point. Let's get this thing knocked down. Go on, see if I can pick it up on camera. I think so. You can certainly hear it. Just rock hard more, doesn't it? So they're not popping off in one brick. It takes a bit of smacking, but... Now you're in a bit. Start hitting it down the wall rather than across the wall. I'll do it in a sec. Yeah. Just stop the wall vibrating as much. That's vibrating right down to the original garage wall. Yeah. Yeah. It's now down there. This is what we're up against. People say the stuff we found always seems to be really bad. That's the lintel, the timber lintel, where the main seating is. Downspout through it. Put it through it. And then that downspout goes down to the pulley down there. So this is coming out. This is coming out. And we're going to put a hole in a bit further up for our steel to go in. And this is coming out. Probably up to about here. Maybe try and find a bend to put on it to throw it out onto the roof so it still drains away. But this as well.  Unbelievable. And then you've got the difference in the pipes there. So you can get your hands under there. Something's a bit quick. You just can't leave it over the tube. And this one here, detached too. It's not moving. Maybe it's mechanically fixed to this one, but it's good. Break time. And I'm not liking the look of that. It is forecast a bit of rain today. It just doesn't cool down yet. So, yeah. So you have to break. Thank you. Right, Alex is up there at the moment. I'll show you from down here. That gutter had a piece. There you go. You've got that. I've glued off a Chinese takeaway and a bin bag on the end of it. And then it comes through both limples. Like that. And then through that stud. And then you've got this two-bottom lintels at different heights, different sizes. You've got bits of 2D2 underneath. A few bits of crackles. You can put them together. And then Alex will show you. Do you think that's held together with just one big nail? Yeah, it's held together with one nail. And there it is. And show me the corner pillar that's come down. Oh yeah. It just came off. It was already loose. It's all shaking. It's very, very weak. Probably lying more to that. Yeah.  Probably get the reciprocal on this nail because I can't get proper leverage to get it out. Whatever. The thing that does the cutting. So I'll get you set up while I do that. And then Dad's going to go pick up the steel, I think. Beautiful craftsmanship. This needs taking back as well. Sure will do. No? No. Oh no, it's just coming out a bit, isn't it? Yeah. Yeah, it should be okay. There you go. Oh, we've got a newspaper. There's your hole. So we're just going to do the same for our steel? No, we've got to come hide and that hole needs breaking up. Oh yeah, but we're doing the same thing, aren't we? Yeah. That timbalinkle. See it's stood? Yeah. It's still a piece. We need to cut the timbalinkle down there so we can take it out and we can break that hole up and form a new hole above it. Okay. Could you grab me the wood blade then please? Obviously just a quick one. This should be common sense, but if you're changing the blades on anything, if you've got a safety switch, just put it on.  I don't know what you're talking about. So safety on, or if there's no safety, just take the battery out or unplug it. Don't bash my arm properly. It is. It's snapped in. So yeah, safety first. The timbalade on because this sits onto the wall at this end. We might have to put a prop under this end, Al, and cut that end off as well. Yeah? Yeah. It's kind of attached to it. That could be left cut back to the stud there. So it's sat on the stud and then we can break up that hole. Break up that hole and make a new hole above it for the steel. And then this end, we need to break on top of this pillar. So we need to chop that back off the pillar as well. So we're going to have to go in. There's an acro inside. Put an acro under that for now. It could probably sit on the door frame. There's a door frame at that end. So we'll see. I'll go around the back another look. So get that end off first and then... I'll cut it up to that. Yeah, anywhere near that. Anyway, even just where the hole is, you can get that piece out. I'll cut your back a bit just so you've got room to work. Mm-hmm. Check there's no electrical wires or anything there first. Yeah. Or if you get the door mechanics. I think it's part of the carriage here. Right, it will come from underneath and come across a bit towards the middle.  It's probably nails underneath it. Hmm? It's probably nails into the stun underneath. Yeah. And this isn't helping either. No. Hit it up. Huh? Hit it up. From where? If you get the ball up there. Ah, it's a nail that I was hitting. Right, I'll leave it. That can stay where it is. Okay. No need to take that out. So we just need to get the two bits of brick out now. Got another blade. Pardon? I need the other blade now. I need to disconnect the wire that's there out the way and take those two bits of brick out and then we're going to be able to mine in the lintel but we should be sitting our steel on top of the porch lintel. Depends if they put one in or not and how far they've gone in with it. That's it, just spin that. The door frame's holding the lintel up. There's no need to prop anything. Well, you have it. There's the steel-sized hole. There's a lintel we've got to try and cut back there. No, don't cut your back. Just go around it. Oh, there you go. Yeah, cut that then. We're going to take them out as well. Oh, are they coming out too? Yeah, you've got to get some neat brick work up there, aren't you? You've got to pretend that hole wasn't there. Yeah. So we've got a few of the abstocks in the back we can do that with. Yeah. The tradesmen. And we're building up the lintel out. I don't know that side of it. There's the steel which I'm going to go and let it do its way in a minute. Yeah. And the lintel's been cut back on both sides and now that Dad's passing me scotch I can get all this polished off so we can get that built back up. Is it sitting on this or? It's going above that. Are we just laying like one course of building the pillar up one more course and two? Maybe even three. To get it above that steel. To get it above that timber. Yeah. Oh, yeah, yeah. No rebuilding there. There you go. That's the bricks loaded up for now. Somewhere on the pallet, but as you can tell I don't really want to take that stack much higher. But that'll give us going for a bit. I was going to put the mix in but we forgot to take the fed back before Dad came. So I'll get that mixed in while he's putting the profile up. I think when Dad comes back with that steel we'll have a quick lunch and then we'll throw this throw everything we've got at this pike. So catch you then. 22. He's loaded the bricks up and we haven't got the steel yet. But when the bricks up the steel was in the steel stockest. But there it is at the end of the drive. That needs to go over here. So that pillar's built up. This pillar's built up. That was where the original tin warm went in. So we're putting our steel in level with the lintel. Alex is a bit worried because he thought we wouldn't get done. He thought it was double face but it's not. It's face with the lock on the back. So it's probably about eight. Two, four, one, two, three, five, six, seven, eight, nine, ten. There's 12 block on that pike but we're coming higher up with our block. So there's probably about ten block at the most in it and whatever brick it is. I could say it's a bit higher in the front because it's a 200 by 100 RSJ with an eight mil plate welder on the bottom for the front, for the face with it. So let's get that up here and get them on top of it. Right, half two. Not doing too bad. There we go. The steel's on. The pillar's raising up. That brickwork's done. Which is awkward but we managed it. It's slightly out of level but that's up. There's nothing to do about that. It's already on massive beds and at that end it's sat on the house lintel. So the only way to get that down is to chop the end of the lintel off which means that that brick should be sat on. 30 mil of lintel. So we just kept it slightly out. It's not a lot out. So yeah, it's set up basically. I'm just trying to get some life back into this mix with some wet sand. So it's done a quick mix. So when you do a quick mix, plasticiser, what the plasticiser is designed to do is trap air. So it's like fairy liquid where you do that in water. It traps bubbles. And when the sand's dead like that, it's been rained on. All the air's been washed out of it so it takes twice as long to let you mix. Come back to life. All this seems to do is just separate and then it's a bit crap really. You see it's all separating. Our mix isn't usually like that. And it's because the sand is wet. Hasn't been caused properly. Another tip. Whenever you're getting some bag of sand and you've got a pallet handy, put it on a pallet. Otherwise it just sits in its own water and the water just soaks up to the bottom. It's on a pallet. The water can run through it and it won't soak water off the floor either. Another little tip there makes a massive, massive difference. Now I'm jointed up and ready to get down. I'm going to go and make myself a brew while that mix, that looks bad now.   And then another two courses and we get ready for the first course of block which Alex can send up when we get. It's built. Just need to get this last bit jointed. I'll go grab the air. Bit of pointing to do there. I'll switch the camera off now and then Alex can do a little outro when we've tidied up. There you go, that should do it. That's tidied up this part and a brick brick done with a pretty hefty steel, but we got it in. Not bad. That's a very weird day. It went our way. I think, like I said before, I think it was this job's way of apologising for breaking us the other day. Yeah, that was a nice, pretty straight forward and all things considered. Not easy, but as you'd hope it would go. Yeah. There you go. So thank you very much for watching. If you enjoyed, be sure to give us a thumbs up and if you're new to the channel, you want to see more, be sure to hit subscribe and tap on that bell to be notified. Any additional support you want to give, you can give us a super thanks which isn't necessary, but appreciated. All support is greatly appreciated and helpful for our channel. So thank you. This has been Piking with Stephen Alex. Then we'll see you in the next one.


Thank you very much, and thank you everyone for being here. The goal of this is really to capture some thoughts as we go forward with the day. So the overview, so obviously GWAS and clinical sequencing are changing how we practice and we'll practice medicine and research, I think that's clear. But what's the levels of evidence that it's required as we move down the road? So we can use traditional QTL to gene and animal models from QTL to gene using GWAS, from GWAS to gene variants, and obviously testing variants of uncertain significance. So we have a lot of expertise around this, and I just want to walk you through one example that's happened in our particular case. So we had a patient that we were actually studying from the Chronic Kidney Disease Consortium. And this individual patient had a mutation in Shroom 3, and this particular variant was a variant of unknown significance. It's highly conserved down through a whole bunch of different beasties. And so the question I want you to think as we go through this is what data would you require to say this variant causes Chronic Kidney Disease to put in a medical record, okay? So that's really the threshold I want you to think about, not to write a paper, but to put in a medical record. There's not going to be, in most of these cases, randomized case control studies to get these variants out there. So that's what I want you to think about as I go through this talk. So Shroom 3. So what do we know about Shroom 3? Well, it's a very commonly discovered GWAS hit. It's one of the most reproducible. Its function is unknown. There's over 11 GWASs that have been hit. And it's been associated with glomerular filtration in Albionaria, and it's associated with all populations. So it seems like a reasonable candidate. However, the evidence in the biology at the time when we got involved in this was it was known nothing about what it was in the kidney. There was basically no information, and in fact the homozygous null mouse was lethal. We didn't have the ability to go walk into a mouse and ask a simple question around that. So this is where we found ourselves. Now the reason we got involved in this is really because of rat data we'd been doing. We'd done QTL mapping over many, many years. In fact, this was a project that I had started with Lander when I was a postdoc with him a long, long time ago. And so Shroom 3 happens to sit in a QTL on chromosome 14. So there's evidence around that. And we went in and took a look at this from the faun hooded rat, which was the disease model, and the brown Norway rat, which is the control. And this is the characteristics. There are glomerular hypertension, protonaria, focal glomerular sclerosis, and potocyte effacement, really characteristic of what you'd find in a clinical environment.  And all of the red variants are variations for which you would say are potentially dysfunctional. So there's multiple variants in this gene that you would predict will cause this protein to break. Okay? So the interesting part about that was how would you attack that? Well, what we did is we then used zebrafish. And we went in and then modeled this. And so what you have to understand is that for renal function the green dye should stay. So when the kidney is working normally, you just keep the green dye. And when you have a dysfunctional potocyte, what you will happen is you'll lose the green dye. Okay? So that's the main element. So if you put a morpholino in for Shroom 3, so you knock out the zebrafish, you actually see that it goes away. If you now put in a normal rat variant, you will now see that you can actually recover that phenotype completely. So you take out the zebrafish and you can replace it with the rat. You now then can show that if you now take these three different regions and we then took these different regions and substituted them into the zebrafish genome for these genes. And what we were able to do then was narrow which part of the rat actually was functional. And what we found using the zebrafish as an assay was that these potential variants were now causal. So we started with rescuing and being able to narrow it down. And we actually got it down to the absolute variant that was causing this disease, if you will, in the rat. So with this information, it was GWAS-nominated. There's QTL data in the rat. Interesting about this is this one QTL in the original mapping. This gene actually is, there's two genes within that same QTL. So in the paper there's the nuances around that. But the point is that it's functionally we know it's causing it. And gene editing in the zebrafish we use to have the causal mutation. So how many people in this room would be willing to write in the medical record now, obviously some of you aren't MDs, but you can pretend you are, would put in the medical record that this is now enough evidence to say that this gene causes renal failure. Okay? One. All right? We'll move to the next. So how about more zebrafish data? Can you take this to more function? Because really I just told you that this variant has a broad effect, right? So what we'd like to be able to do is take this into a deeper depth. And so what we did then is we used the zebrafish, and now what I want to talk about is being able to go in and look at podocyte effacement. That's one of the key characteristics. So zebrafish, although it's a pro nephron, does have glomerular filtration membrane and does have podocytes, which you can see listed on the slide here. So we then went in and did a series of studies that I just showed you before looking at the green fluorescence protein labeled and seeing what was happening. Sorry, this is dextran. And so here we went in. We did the same study. We're able to show that we can cause skipping. We're able to go in and show that there's shroom 3. We can knock it out, and we can show that it has function. So then what we did is we did a really interesting strategy. That's just to tell you how this works. Hypothesis that it regulates the filtration barrier. So this is the strategy. So we then went in and we now used a Gal 4 mutation. So we got this variant from Ian Drummond, and we're now able to go in and take a podocyte specific and be able to say, okay, we're attaching it to podosin, and now we're only going to express and be able to knock this out specifically. So now we're showing, if you express this gene and it's broken, it should cause podocyte effacement. And sure enough, it causes podocyte effacement, and we can show also in the protein area. So now we've been able to say, okay, it's not just this particular variation, but we can take it all the way down to showing that there's podocyte effacement, which is actually what's thought around this. So now we've added more information. So now that we can show that in zebrafish that there's podocyte effacement, how many of you now would say that this is what causes the gene in humans? Okay, could cause. Okay, we're right in the medical record. Let me say that. Stay with the same question. How many, besides Terry, anybody else would add that we got one more person, okay? A few more people, okay? All right. But this patient already has kidney disease. Putting it in their medical record is not such a big deal. It's how you're going to interpret it for the future. Keep that in mind. That is our, that is a position. Okay, so keep that in mind. How about if we just test the patient's variant? Wouldn't that be a really good idea? So that's, guess what? We did the exact same strategy and we now took this patient's exact variant. And we showed what I showed you before. And now what we did is we went through and we actually went in and we took a common variant from a human and showed that it rescued. So we rescued the phenotype and we made the exact single base permutation from this patient and showed that it caused the disease in the zebrafish, okay? So, same argument. Would you write this in the medical record? Okay, so we've been through this exercise. Now let me tell you the interesting part about this. I presented this at the American Society of Nephrology in November, okay? And I asked this exact question. So of 500 clinical scientists in the room, how many of them do you think agreed that they would write this in the medical record for this patient with this data? Zero. Zero. In fact, what I got were four people that stood up that told me that a zebrafish is not a human, a rat is not a human, and there's now data that's also come out that I'll show you next that a mouse is not a human. And so it doesn't matter how many times you do this without a randomized case control study, we are not going to write this in the medical record, okay? So the reason I'm telling you this story is that this is a group of people who were trying to figure out how to do this. Now there's a lot of education and the point of this is really to illustrate what people think. This is a paper that just came out. I don't think showing that a mouse would either convince people. So the point that I wanna make, the simple point in setting the frame of this meeting is, I think that in this room, we think about sequence first, ask questions later is gonna drive a lot of basic research. I think basic research at the speed of the clinic is gonna be absolutely essential. But how do we define proof? Now obviously there's risk benefit, the sicker the individual, the more we're willing to do this. But as we walk into this meeting, I just want you to think about that, at least from one perspective, if we're going to try to affect care, the data upon which we're trying to drive needs to demonstrate enough evidence, whatever that is. And so that was the only point that I wanted to make. There's a lot of people that were involved in this. My former laboratory up at the Medical College of Wisconsin, Flo, actually this was her thesis work. She's now a postdoc with George Church, a large number of people that I've worked with across the team, and then also the chronic kidney disease. So I'm happy to take questions. I'm not sure there's a lot of questions around this, but I'm really more about setting the stage of where we're going for the rest of the two days. Thank you. Thank you, Howard. So Rex first, Mark next, Nancy next, less next. You'll be happy there are no, any questions, right? So I think this is a really great way to start off this meeting because it really does highlight what it is that we're trying to think about. In the minds of those 500 people in the audience, is how do we ever get over this idea that you need some kind of a randomized control study in order to do that? Because that's going to be a barrier that we're never going to achieve. So, thoughts? I have to, I have to be completely honest. So I was shocked. Okay, I didn't expect, I didn't expect a huge number of people to actually stand up and agree with us. But I was shocked that nobody was willing to raise their hand that they would write it in their automatic record. As Nancy said, hey, this is a patient. We already know there's a mutation that's there. You've gone through all of this elaborate biology. I expected some agreement around that. So I honestly don't know. And that's why I think this meeting is incredibly important. Now, I'm a PhD. So I have a different mindset on this. And I think that's why this is really important to have the physicians in the room and the basic scientists around the room. Because if we can't convince the physicians here that I'm going to argue are more willing to think about it, then I think we can't take it out to the general population. But that's just an opinion. So my question was related to what I consider to be a huge pile of missing data that as a clinician, I would need to understand to be able to interpret the functional data. And that is, what's the allele frequency? Has this ever been seen in any of the exome collections? Is there any evidence of segregation data? You know, are there other individuals that have been reported with different mutations in Shroom 3? Those are the types of things. And it may be a null set. But I think, you know, with absent any of that other data, the functional data while convincing would certainly not be enough for me to be definitive about causation. I might suggest, you know, that we could put something in there to say, well, there's a variant in this that's of uncertain significance. It needs additional study. But if you think about the implications of making that assignment, since presumably this is now something that you're proposing as an auzomal dominant, now you're thinking about cascade screening and all the other impacts of this. And so, those are the types of things that I need to be able to contextualize it. I can't make a decision based solely on functional data. So, short answer, Howard, and then we're going to stop. So, for the sake of clarity, which I didn't get into this. So, chronic kidney disease is a polygenic, multifactorial disease. And so, in this particular case, there is evidence in the population of Shroom 3. That was the GWAS data. So, the challenge on this is a little bit harder than what we're talking about for the Mendelian disease and that it is, in fact, only one of the elements. It is no way the only element. And it will be entirely possible, and I'll even predict, that in the cohort, there are going to be people that have a mutation in this gene that do not get to the disease because there's complementary. So, the complexity of this is a little bit more because it's a common, complex disease, common in the sense of there's more than 200,000 people that have this. But even with that information. So, I think those are fair points, and thank you. I think that at least stimulates, I hope, the conversation over the next few days. I think we could probably sit here and talk about that presentation for the rest of the day. But we won't. So, Marty Westerfield will give the basic science perspective on the need for integration. And if you want to know anything more about him, read it in the biography.


I'm the author of Malignance of Lava, Narcissism Revisited. Most victims of abuse commit a serious error. They attempt to present to their children a balanced picture of their relationship and of the abusive ex-pulse. In a vain attempt to avoid the notorious and controversial parental alienation syndrome, these victims do not besmirch the abusive parent, and on the contrary, they encourage the semblance of a normal, functional liaison. This is absolutely the wrong approach. It is not only counterproductive, it sometimes proves outright dangerous. Children have a right to know the overall state of affairs between their parents. They have a right not to be cheated, and not to be deluded into thinking that everything is basically okay, or that the separation is reversible. Both parents are under a moral obligation to tell their offspring the truth. Their relationship is over for good, and there is a guilty party. Younger kids tend to believe that they are somehow responsible or guilty for the breakdown of the marriage. They must be disabused of this notion. Both parents would do best to explain to them, in straightforward terms, what led to the dissolution of the bond. If spousal abuse is wholly or partly to blame, it should be brought out to the open, and discussed honestly with the children. In such conversations, it is best not to allocate blame. But this does not mean that wrong behaviors should be condoned or whitewashed. The victimized parent should tell the child that abusive conduct is wrong, and must be avoided. The child should be taught how to identify warning signs of impending abuse, sexual, verbal, psychological and physical. Moreover, a responsible parent should teach the child how to resist inappropriate and hurtful actions. The child should be brought up to insist on being respected by the other parent, on having him or her observe the child's boundaries and accept the child's needs and emotions, choices and preferences. The child should learn, in other words, to say no, and to walk away from potentially compromising situations with the abusive parent. The child should be brought up not to feel guilty for protecting himself or herself and for demanding his or her rights. Remember, an abusive parent is dangerous to the child.


Frog kicks sit on a bench and pull your knees into your chest and extend them again You may hold the bench to stabilize yourself, but prevent pulling with the arms reps 15 to 20 Please note. You can do this hanging from a chin up bar Hey, thanks for watching my Bruce Lee training and workout video There's a lot of videos in this series So make sure you check those out too if there's your first time here Make sure you click the subscribe button and get the latest workout martial arts fitness and self-defense and fighting tips All right, so we see you in the next video


Hello everybody. Andrea Mayesca here with dental tutoring. We are going to be talking about something a little bit different today So not tutoring not students necessarily, but talking about how to ask for a raise Yes, I said it so you might be wondering how am I one to talk about asking for a raise? How am I supposed to help you guys? Well, I'm making a video on it actually because I used to work in an office for about 12 years and Let's just say they didn't just offer up a raise to you You had to ask for it and a lot of us and it's normal Don't feel comfortable asking for money. You know a lot of us don't feel comfortable asking for more money A lot of us don't feel comfortable asking for a raise But if you don't ask for it, you might not get it even if you've been working there for 10 years It's not unheard of for people not to get a raise for 10 years And I think that's crazy and I don't want anybody to go through life and go through work Doing that because believe me there are other people in your office Asking for a raise and getting it. Okay, that shouldn't be the way It just should be offered to you every year every two years based on your performance, of course But if you're doing well, it makes sense to get a raise, right? Right because we all know the cost of dental goes up every year the fee guy goes up every year So why not reflect that and pay your employees accordingly, but we are not here to knock the dental profession So let me tell you guys how you can ask for a raise plain and simple And these are real-world tips for you that I have applied that I have told my colleagues to apply and it works They always get what they want. Maybe not right away, but they will okay So if you're in the category where you haven't gotten a raise for several years And you know you're being underpaid. This is actually the easiest thing to do and the easiest way to get a raise Even if it's now and you just had your performance evaluation or your performance Review or let's say you're working in an office where you don't even have that Now is the time to ask for one So whether you feel more comfortable going to the office manager or going to your boss So probably the dentist yourself that's up to you, but you need to go to somebody So probably your boss probably the dentist and say to them. Hey, you know what? I would love to talk to you About my performance here I think it's something that we should talk about because I would like to be the best employee that I can be Say something along those lines, you know Say it so that you want to improve yourself and you want to talk about your work So that you can improve and do whatever you can to make the office better Don't march in there and say listen. I need a raise I haven't had one for five years if you don't give me a raise I'm out of here, you know, you may be thinking that but don't be saying that right because that's not gonna help So go in there acting as if you owe them something and that you wanted you the best that you possibly can't Now if they stop you and nip that in the bud and pretty much say you you know what? I don't have time or sure. Let's book an appointment in two months or sure Get back to me at the end of the day because we're kind of busy right now Like you know what I mean? If they try to sweep it under the rug Depending on when you go to them if you're going to them in the middle of a patient not a good idea I should have mentioned this at first go to them at the end of the day or at the beginning of the day Before they're seeing patients or even before you do that say to them Um, do you have a moment to talk because I need to talk to you about something? They don't know what it's about. So if they have a moment, they will say yes If they if they just honestly don't have them have a moment then say to them, okay, no problem Um, can I talk to you at the end of the day? Is that okay? Okay, great. Perfect. Thank you So then just talk to them and you know ask about getting and talking about your performance But don't talk about yourself talk about them first start off and say something like, okay How do you feel I'm doing? You know, I just I honestly want to know Whether you've been there for six months two years ten years. It doesn't matter Say, how do you feel that I'm doing and you feel I couldn't prove with anything? even tell a little white lie and say I've been taking Continuing education courses on the weekends. I am having such a good time doing that and one of them was you know Forcing us to look at ourselves and how we can do to improve. I don't know say whatever you want And that's why I'm talking to you about it. Okay, so you're not talking about a raise, but you're talking about your performance So this is when they will likely say, oh, well, you're doing a great job. Thank you for always being on time What whatever even if they don't say all good things Take it back and say, oh, okay. You know what I I apologize that I was late that one time Who knows right say, oh, you know what I apologize that I was late that one time this happened, but that's no excuse It won't happen again. Um, but I appreciate you telling me and being honest You know just talk to them talk to them as much as you can right And then say well, you know what I haven't had a raise for about five years two months, whatever I would like to talk to you about that. Um, do you have time to talk about it now? You know just You'll know more how the conversation is flowing and how the conversation is going if they seem open Keep on talking if he's kind of he or she is kind of like, oh, you know, I got it Sorry, I think I have a patient. I just I got to go hold. I can't hold If they're if they seem distracted if they don't seem interested It's okay. You guys it probably took them off guard that you are being assertive Okay, and that's fine say. Oh, you know what? I don't want to take up too much of your time Um, can we meet again after work and talk about this or can we meet again on thursday? And talk about this don't leave that room or that area without a time a place to talk about it again Okay, make sure to get that half an hour appointment or whatever But if now is your time Simply say to them. I haven't had a raise in five years. I would love one. I love working here Um, how can I go about that? That's all you got to do And then just look at them. Don't say things like oh, you know, I kind of want to raise It's been about five years if that's okay with you. Can I have a raise?  It's been six months. I don't know. Hey, it's okay Say I would love a raise. It's been two years. It's been five years. It's been 10 years What are your thoughts? You know, let's talk about it, you know If they say well, you know, I I would like you to improve this and this and this Or if they say well, it's not really in the budget right now I'm sorry. We're not giving anybody a raise at the moment That's the worst thing that a dental office can say because there's always room in the budget. Okay Seriously, they make enough money to give you a raise Especially if it's been like 10 years or even five years even two years you guys you should be getting a raise every year But if that's what they say This is what you say back. Say, okay, I do understand that even if it makes no sense Say, okay, I do understand that. Um, let's talk about it in three months In the meantime, is there anything that I can do to improve to get that raise from you? If they're still standoffish and say well, you know what there won't be room in the budget. They're just They're just isn't room. I'm sorry then Turn the tables works every time Say to them. Oh, okay. Well, you know what? Okay. I do understand it's not in the budget I do understand having to budget completely Um, then in that case, I would like a day off During the week or I would like additional benefits Come up with something that works for you There are a lot of you out there that would honestly just love a day off But there are some of you out there that are saying I can't afford a day off Then don't ask for a day off But ask for something else The reason why I am asking you to do that is because I'm hoping they will say Oh, okay. Well, I can't give you a day off or well, I can't give you benefits or I can't negotiate better benefits. Well, they have to do something for you, right? Then you can say, okay Well, is it is it easier for you to give me a raise then because it's either I would love a raise Or I would like benefits now if you don't have them Ask for benefits. Ask for additional sick days. Ask for a day off Ask for something They have to pick one. They don't have a choice So if they do say to you, oh, well, we can't afford to give you sick days or we can't afford to give you benefits Or are you taking a day off isn't an option then say to them Okay, well, what's the best option giving me a raise or picking one of those options Because I would like something you just said that I'm an amazing Employee I've been here for five years. I love what I do You're awesome. You know put some humor into it What's you know, what can we do? They have to pick something it works every time It does It really does you guys it works every time So what I want all of you guys to do is to try that If you have any questions comment below when I can help you Feel free to share your experiences too so we can help each other and I will tell you guys How to go back if you didn't get what you want How to go back and get what you want I'm willing to put money on it, you know Like this is how confident I am and that if you do what I say you will get a raise Or at least something that you want Um a day off benefits extra sick days able to come in two hours later to take your kid to daycare Whatever You will get what you want, but I'm hoping it's going to be more money So comment below and let me know your thoughts and even share your success stories of if you have asked for a raise in the past Because I did do a video on this about a year ago I think another one about six months ago, but this is just more of an updated version So if you guys need help if you can have questions Let me know and good luck because I know for me it's worked every time and I've been doing this for uh Yeah, about 14 years the colleagues that I've helped it um works for them every time So one of my more recent colleagues she didn't get a raise for 18 years Yeah 18 years, but that's because she didn't ask for one. Well, she did call in sick a lot. She was awful a lot too so fair enough for her 18 years to you guys and she got a raise finally After I helped her out So I know I could help you out too. So let me know what you guys need and I'm happy to help I'll see you guys in the next one