like that this isn't made of metal it's made of wood so I have to do the sword coming unsheathed noise on my own also hi this is the third time I have filmed this so if I sound tired and annoyed it's because I'm very tired and very annoyed this is the introduction song it's not very good but it's not too long okay so Empire of Man I recommended the first book in this series a couple of months ago it was a March Up Country I did a short saying hey this is good and at that time I had only read the first book now I've read the whole series and I can confidently say yeah it's still really good it's a brilliant piece of military science fiction it's about a prince who is traveling through space with his contingent of marine bodyguards and they wind up getting stranded on a backwards barbarian planet which does have intelligent life on it but they've barely invented gunpowder and they need to walk like halfway across the planet from where they land to the only spaceport there so that they can finally get home and it takes them literal months and the whole way through they're fighting they're trading they're negotiating they're gathering allies it's like you might hear the term military sci-fi and think okay it's just an endless series of battles but no not at all and while this series doesn't quite stick the landing I'll talk about that in the spoiler corner it is still really good you know it's still a very solid adventure I say adventure but that always feels weird when talking about stories like this which are pretty serious and at times very grim but it's also the best word I can think of like it it's a very good adventure has a lot of heart to it more than you might expect and I just I really enjoyed it so this series is four books long it's March Up Country March to the Sea March to the Stars and We Few and the first minor spoiler the first three books are the Marines and Prince Roger just traveling to the spaceport and then the last book is actually where they realize okay even after we get off planet we're still gonna be in trouble because of some shenanigans that have gone down and so that's them dealing with that but the vast majority of this series is them trapped on the planet it's called Marduk and the people there are called Mardukans by the way and it's mostly just them traveling to this one goal and if you think that it sounds I don't know unsatisfying to have characters just be walking and occasionally running into trouble for three books I mean if that's not your thing that's not your thing but that's also what Lord of the Rings is you know when you distill something down to its bare essence and strip all context from it yeah it doesn't sound that good and like I said this isn't just an endless series of battles because again you hear Marines with advanced technology going through you might think okay they just shoot their way through every problem but no because their technology gives them a massive massive advantage yes but number one they're snuffing out sentient life like Mardukans again they are intelligent they have civilizations they're just not nearly as advanced as humans at this time and number two they do not have the resources to just power through everything you know like sure the Mardukans are mostly resort mostly stuck to using swords and spears against them with their advanced rifles and cannons and such but they don't have infinite ammunition and also there are times where the Mardukans can just overwhelm them with numbers and the Mardukans have distinct physical advantages which I'll talk about a little bit in a minute now if you don't like military sci-fi just as a genre number one I disagree with you because I really like it I think it's a very fun genre but if you don't like it I don't think Empire of Man will change your mind because even though again there's a lot of stuff besides battles in there it is still largely focused on them you know it's focused on battles it's focused on the weapon technology and how it works it's focused on tactics it's focused on logistics like just the ways that wars work which is what people who read in the genre are going for I don't know why my camera is like tracking this thing's face for some reason but it is it's doing it and it's really bothering me I'm looking at the little screen recorder here and it's it's irksome what sets this apart from a lot of other entries in the genre though is the fact that it is much more character focused because that's always been one of the weak points of military sci-fi and I think a big part of what turns a lot of people off from it is that characters are less than two-dimensional a lot of the time like you won't even get a hair color for a lot of the main characters here like they are just people who are there to be idiots be hyper competent or to fight oftentimes they're just there to do stuff rather than to be any sort of real characters they don't have much personality they don't go through development really and so yeah if you're just looking for explosions and such then it's still a fun genre to get into but if you're looking for something a little more substantive then yeah that can be very frustrating that said Empire of Man again does not fall into that trap because this series has a pretty large character cast and it is full of all kinds of really distinct memorable characters like the main one to talk about is Prince Roger himself he's the protagonist of the series he's pretty young at the beginning he's only like 22 or 23 and he's also not the heir to the throne like his mother is the Empress but his he has two older siblings and his brother has children so they are all in the line of succession ahead of him so he doesn't seem that important and people also just don't like him and it's hard to see what it's hard to not see why you know because one they go into some details regarding like political maneuvering and his father later on in the first book but also he's just kind of a sour-puss and he's kind of annoying to deal with to the point where even his bodyguards hate him but over the course of the story he becomes like a proper leader and you realize like okay yeah I would be okay with this guy being a prince and being in charge of something now part of that is realizing that he wasn't that bad to begin with like realizing okay yeah he had a lot of useful stuff to say and do all along and people just didn't give him the chance like you realize okay he's he's pretty smart he knows how to fight pretty well he is an amazing sharpshooter because before the events of the books he actually went big game hunting on a bunch of planets and was hunting down very dangerous aliens all the time so you know he knows what he's doing and he is much better suited to this long-ass trek through the wilderness than people gave him credit for but also he does just get better over the course of the story you know he learns and grows and just becomes more conscious and conscious conscious might be a better word for it that was hard to say of the fact that people's lives are riding on him and his responsibility is to them just as they have a responsibility to him so again you really get the feeling that yeah this guy is good leadership material however they do occasionally go overboard with it and make it so that Roger is like not at all a Mary Sue because he does screw up plenty but he's just a little too good at some things like they mentioned he's a Kenjutsu expert at one point and it doesn't even make sense for him to be an expert because it's mentioned that he skipped a lot of his classes and kind of tried to blow it off but whatever and they mentioned that okay at one point in the books they start picking up Marduk and weaponry because they're trying to conserve ammunition so the humans start using you know spears and swords and such and one of the weapons they pick up is a really big knife now Marduk ins are substantially larger than humans they also have four arms like on average they are over three meters tall maybe three and a half on average and so Roger picks up what to a Marduk in is just a particularly big knife but to a human it's the size of a sword that's why I actually have been holding this thing this whole time and Roger is able to use it as a katana and okay again he's gone through some training and stuff I believe that but he is able to fight these battle hardened Marduk ins who have been training and fighting as warriors for years and he's able to fight them one-on-one and come out on top almost every time like he is just a little too good at this you know what I'm saying and again consider how big something would have to be for this to just be a knife to them and you realize how much of a disadvantage a person would be at in terms of like reach and strength and size and it becomes even more unbelievable and the only other character I want to talk about in any depth is the leader of the Marines whose name is Captain Armand Ponner and he's great too you know he doesn't have as much depth to him as Roger I don't think but he is still very fun to watch because he's very very good at what he does he's not just a brilliant military commander but he's a great negotiator and I feel like in another life he would have been an amazing politician or king or something you really get the feeling that nobody else could have led these guys through all these difficult dangerous situations than Armand Ponner and so watching him do his thing is great but he's also not perfect he does occasionally screw up and there is a human underneath that you know he does feel fear and anxiety and stuff he just tries not to let it show and throughout all of this you really get the sense that he is just undyingly loyal to the Empire and by extension to the Imperial family including Roger himself so even though he really doesn't like Roger at the beginning of this series he will do anything to keep him alive because that's the job he signed up for and other than that I don't want to go into a lot of detail about the character cast but just know there are literally dozens of them you know good guys bad guys they have big roles and small roles there are humans mardukens there's a couple of other alien species we run into and that there's dozens of them and they are all very distinct and a little bit memorable that's why I'm saying like this is much more character driven than most military sci-fi and I'm gonna remember these characters for a while like I'm gonna remember Kord who is a marduken he's an honor-bound warrior who winds up leading or excuse me not leading but guiding the humans pretty early on in the story but also in spite of him coming from a literal Stone Age tribe he's one of the most intelligent people in the series and he adjusts remarkably quickly to his new circumstances like I remember Portenya who is the armor that the Marines bring with them and how he's very foul-mouthed and the audiobook narrator gives him a mildly racist accent let's not let's not get into that and how he's just remarkably good at his job and how he knows not just about armor stuff and stripping down their technologically advanced weaponry and such but he also knows about carpentry and other things that come in handy over the course of the journey and I remember how he's just a really crazy gambler who is constantly trying to meet new people that he can teach new games to so that he can beat them and win money from them like that's just a thing he does I remember that there's a lot of these you could I could go on all day about them like even the villains got some weirdly poignant moments and in spite of all of these people all having personality none of them are safe you know because there's a lot of stuff where people have said excuse me it feels like oh no one is safe anyone could die at any moment in the past few years that has just become a popular thing but in this series it really does feel that way because again there's a lot of characters who we all grow to know and at least like a little bit over the course of the story but on top of that a lot of them die along the way you know it's not just faceless grunts who get killed during these many many dangerous battles it is named characters it's important characters who you grew to like and sometimes they go out in a brilliant blaze of glory and they like hold the line and prevent the enemy from coming in and allow their friends to retreat before they finally bite it and they get this big heroic last stand other times they just die very unceremoniously off-screen and the other characters just come across their corpses and they're like oh yeah they they just died I haven't talked much about the battles but suffice to say they're all pretty great like there's only one or two big ones per book with a couple of smaller ones thrown in throughout but they're all great because none there's not a single one where the humans just blast their way through with pure brute force like they have to think their way out in just about every situation because again they're not just trying to blast through this one group they realize they have a very long journey ahead of them and as time goes on they run lower and lower on resources like food and ammunition and they take more and more casualties so there's just less of them to deal with it and it's just it becomes more and more desperate so the fights continue getting more and more tense even though in some ways the characters are just more prepared because they're more familiar with the planet and everything and none of these are just battles for the sake of having battles the final goal is always in sight and it's always clear they're there to get to the spaceport and get off the planet and then after that they have a different thing now I've been saying this book's praises for a while or saying this book's praises is it saying or singing the praise whatever not important I've been singing this book's praises for a while now what are some of the issues I have with it well number one is that there's just way too much exposition here like some of it is important to the story but a lot of it isn't and it goes on for a very long time like I thought I was going to be in for a rough ride when early on in the first book one character shoots another and it spends what must have been like half a page just describing how their gun works and I was like is this whole series gonna be like this and yes but it's not as bad as I feared it also takes a while for the story to really get going because I think it's about 20% through the first book before the actual story begins like before the Marines and Roger crashed on the planet Marduk and like again that's the setup that's just the inciting incident for the main plot the main journey so again it could have been condensed a bit I feel like David Weber maybe got away from his editor a bit and the only other issue I have is again the ending which I'll go into a spoiler corner but suffice to say it's just it feels unfinished you know it feels almost like there's supposed to be another book but there aren't any but so it just it just ends there and so it just doesn't quite work I didn't think but that's all you know other than the ending kind of falling flat and a few issues throughout I thoroughly thoroughly enjoyed the Empire of Man series if you are at all a fan of military science fiction I would definitely recommend checking this one out if you don't like military science fiction I don't know if this will change your mind but you know the journey of Prince Roger is still very satisfying so maybe it will and if you're kind of on the fence about military sci-fi then I would definitely recommend giving this a go you know at least try it out and if you're just not into it by the end of the first book then you're probably not gonna be into the whole series but anyways that's about all so spoiler corner now okay the only thing I really want to talk about in this spoiler corner is the ending and in order to do that I need to give you some context so once they get near the end of the third book that is when the Marines are starting to approach the spaceport where they can get home and that's when they run into a couple of humans who have been staying on the planet Marduk and are just nearby the spaceport and that's where they learn that not only have they been presumed dead ever since their ship got shot down which they assumed for a while because they've been on the planet for like eight months at this stage not only that but while they were gone there was a coup or officially it was an attempted coup where some people tried to overthrow the Empress but they managed to hold them off and they managed to save the Empress and now she's totally not being held prisoner guys she's just away from public and you can't see her and these other people are totally not running things in her absence and you know so officially the coup failed but in reality it succeeded they take the Emperor prison Empress prisoner and they kill Rogers older siblings and his nieces and nephews so now he is the heir to the throne but everybody also thinks that he's dead and at the end of the third book while they're hijacking a ship so that they can get home Captain Ponner is killed and again it happens rather unceremoniously kind of out of nowhere it's not a heroic last stand or anything he just gets very unlucky and it's actually the saddest death in the entire series even though the series is full of like I said earlier a lot of very sad poignant deaths but Roger realizes okay I need to do a counter coup so that I can overthrow the people who are holding my mother prisoner and hopefully free her and then I guess I get to be Emperor one day and the fourth book is mostly just them doing that so like they do a lot of preparation they gather up allies they gather up weapons they scope out the political landscape and figure out how exactly they're gonna do it and then at the end there's like a big battle where they go into the palace they fight off some of the bad guys and they capture or kill some of them but the main one who was leading the Empire in while his mother was all drugged up and held prisoner he manages to escape and he goes back to his home system where he has a base of support and he actually has the support of a big chunk of the military as well and so you realize okay that there's gonna be a much larger conflict which arises from this like the Empire is just in a state of civil war but they do manage to rescue the Empress like I said but because of everything she's gone through she's just not mentally fit to be in charge anymore so she abdicates and leaves Roger in charge and then Roger is the Emperor he inherits a civil war and that's it that's where the series ends and it's like I said it feels unfinished because Roger like I guess what they were trying to go for was to show how this whole story was just the journey he took to becoming the type of person who was worthy of being Emperor and then it ends on kind of a note of yeah and then he ruled for many more decades and everything was great but I don't know again it just feels unfinished because there's like a whole war going on and everything I don't have anything else to add there really it's just unsatisfying and I would have felt weird if I didn't mention it but yeah that's about all I have to say again check out the Empire of Man series if it sounds at all intriguing subscribe to this channel and like the video and comment on it if you haven't already and also go subscribe to my second channel the other James I don't really talk about it but it's in the description of all my videos so go check that out that's where I rant about random bullshit that isn't really related to books and I think that's about all so I'll see you later good bye hey guess what well I mean you can probably already guess what you don't need to actually guess whatever all these names here these are my patrons these are the guys that send me money once a month over on patreon if you want to get access to stuff like early videos and occasional exclusive content then go ahead and go over there and help me out my $10 and up patrons are aposavillain and Olivia Rayan brother Santotys Buffy Valentine Carolina Clay Chibs Ahoy Dan Anceliovic Dark King Dio Echo Flax James M Carcat Kitsune Lexi DeLorm Liza Rudikova Lord Tiebreaker microphone missed boy Mitsimona peep the toad Robi reviews sad martigan psych xs sillier the vixen stone stairs Tesla shark they victus vimex soul and Wesley how could we ever forget Wesley thank you all so much you're all the best I couldn't do this without you if you want to get your name up here go over to patreon if not subscribe to the channel like the video comment on it you know all the stuff that we are supposed to say at the end here I love you I don't actually love you I don't even know you but you know thanks goodbye


My name is Chris of FilmsByChris.com and this is a test video for tutorials later this week where I'm going to show you how to do overlays in blender.


What's going on y'all Chris from Jasper's case breaks calm back again with another filler 2021 prism baseball sell-up pack random team number three the new word the five across that out. It's three one two three nine spots for 2016 optic baseball given away as well that We'll have a three now and then four right after this That will unlock the optic baseball then we can do another break after that But here are two dice are one to 30 from Logan down to Carl and our teams d-backs down to Nats Rolling two dice for both spots Four in three is seven so seven times. Good luck everyone got one two three four five six and last one seven I slogan down to Michael And then for our team seven times good luck two three four five Six last one seven right here Marlins down to white socks. I'm just gonna get right to ripping two right Here we are Here's our one pack Prism baseball. All right. Good luck everyone Jared tonic rookie nice one elimination Joe Adele Green hazes, was Arda and a champion stitch to each brand.  Those are our pack. Let's do our randomizer for the eight spots Our nine spots. So let's get back to our randomizer. Let's grab our spots here We're showing the one time for the optic let's roll our dice three and a six is nine so nine times Good luck everyone One two three four five six seven Eight and our last one nine. Good luck when the nine So 10 to 30 David down to Nicholas. Thank you y'all appreciate you can this break out. Unfortunately, you will not be in our break for in our next Optic Diego Diego Carl Steven David Carl Logan Diego and Michael So those are our nine congrats y'all. Thank you very much once again for getting that filled out that filler That's nine spots going into our optic right after this. We're just gonna do right hop right back into opinion prism number four And I'll get our optic break done. Thanks very much once again y'all be right back for it Chris from jashby's case breaks calm


Live from New York, it's theCUBE. Covering AWS Global Summit 2019. Brought to you by Amazon Web Services. Welcome back, here to New York City. You're watching theCUBE, the worldwide leader in enterprise tech coverage. I'm Stu Miniman, my co-host for today is Corey Gwynne, and happy to welcome to the program a first time guest on the program, Shes Parthovi, who is a senior leader of global business development with healthcare life sciences genomics group at AWS. Thanks so much for joining us. My pleasure, thanks for having me. All right, so, you know, we love digging into some of the verticals here in New York City, of course, we spent a lot of time on the financial services piece. We actually had another one of our teams out at the AWS Imagine show going on yesterday in Seattle with a lot of the education pieces. So healthcare, life sciences, and genomics. A little bit of tech involved in those groups, a lot of change going on in that world, so give us a thumbnail if you would as to what's happening in your world. So, well, just from a scope point of view, healthcare includes life, pay your provider, life sciences is far more biotech, it's both a medical device and then genomics. And what we're seeing in those spaces, let's start with healthcare, it's such a broad thing, we'll just sort of go back and forth. In healthcare itself, what we're sort of seeing that customers ask us to focus on and to help them do, sort of falls into three categories. First is a lot of customers ask us to help them personalize the consumer health journey. You and I, all of us are sort of accustomed to the frictionless experiences we have elsewhere and in healthcare there's a lot more friction and so we're getting a lot of inquiries and requests for us to help them transform that experience, make it frictionless. So an example of that would be if you're familiar with ZocDoc, I think I started out of here in New York actually, when you want to book an appointment at ZocDoc, you can, normally if you go online, I have to put in your information for insurance, you have to type it all in, it's full of friction, you have to put all the fields in. They use one of our AI services called image recognition and you simply hold up your cards to the camera and you're able to pull your insurance information determine eligibility and book the right appointment for you. So that's an example of removing friction for the consumer of the health consumer of the patient as they're trying to go through that health journey. So that's sort of category one, frictionless experiences using AWS to support it with AI services. So in category two, we're getting a lot of interest for us to help health systems predict patient health events. So when you think of value based care, the way you actually are able to change the cost quality curve is by predicting events not just dealing with matter of the fact. And so using AI ML services on top of data to predict and forecast events is a big part and one example would be with CERNR where they moved their healthy intent platform which is a longitudinal patient record platform onto AWS about 223 million individuals that are on that platform. And we did a study with them where we consumed about 210,000 individual patient data and created a machine learning model and this is published where you can predict congestive heart failure 15 months in advance of it actually occurring. So when you look at that prediction and forecasting, that's sort of one of the powers that have been springed to that sort of category number two is predicting health events. And then the last one I'd be remiss in leaving out is that you probably have heard a lot of discussion on physician and clinician burnout. So the frustrations of whether nurses or doctors and most of the time that part of that is not having the right information at the right time to take care of the right patient. Data liquidity and interoperability is a huge challenge and a lot of our customers are asking us to help solve those problems with them. You know, at HIMS this year, we announced together with Change Healthcare. Change Healthcare said they want to provide free and probably to the country and AWS with the platform supporting that. So those are sort of three categories. Personalizing the consumer health journey, predicting patient health events and promoting interoperability as sort of the signals that we're seeing in areas that we're actively supporting our customers in sort of elevating the human condition. It's very easy to look at the regulation around things like healthcare and say, oh, that gets in the way and it's onerous and we're not going to deal with it or it should be faster. I don't think anyone actively wants that. We like the fact that our hospitals are safe, that the healthcare is regulated in some of the ways that it is at least. But an artifact of that means that more than many other areas of what AWS does is your subject regulatory speed of feature enhancement as opposed to being able to do it as fast as technology allows. Relatively easy example of this was a few years back, in order to get AWS to sign a BAA for HIPAA certification, you had to run dedicated tenancy instances and well, that changed about a year and a half, two years ago or even longer, depending it all starts to run together after a time. But once people learn something, they don't tend to go back and validate whether it's still true. How do you, I guess, find that communicating to your customers about things that were not possible yesterday now are? Yeah, when you look at HIPAA eligibility, so as you know, AWS has about over a hundred HIPAA eligible services, which means that these are services that, so compliance, let's just start there. Compliance, remember, is an outcome, not a feature. So compliance is a combination of people, process, and platform. And we bring the platform that's HIPAA eligible and our customers bring the people in process, if you will, to use that platform, which then becomes complying with regulatory requirements. And so you're absolutely right, that there's the diffusion of sort of understanding of the eligibility of platform and then the work that the customers have to do in order, as a shared responsibility to do it, that diffusion is sometimes slower and in fact there's sometimes misinformation. So we obviously work with our customers in that shared responsibility model so that they can meet their requirements as they come to the cloud and we can bring platforms that are eligible for HIPAA so they can actually carry out the workloads they need to. So it's that model, you know the way I think of it is this, when you think of compliance, is that if I were to build for you a deadbolt for your door, and I can tell you that this complies both sorts of things, but you put the key under the mat, we might not be complying with security and regulatory requirements for our house. So it's a shared responsibility, I'll make the platform be eligible and compliant and so that collective does take time and it does take people understanding that there is a platform that's eligible and then they have to also in their response to work through the people in process portion to make the totality of it comply with the requirements for health care and regulatory requirements. Some of the interesting conversations I've had in the last few years in health care and the industry is collaborations that are going on and how do we share data while still maintaining all of the regulations that are involved? Where does AWS get involved there? Sure, that's a fact, there is data sharing part of that data liquidity story that we talked about earlier in terms of interoperability. I'll give an example of where AWS actually actively working in that space. You may be familiar with a service we launched last November at DreamVent called Amazon Compian Medical and Compian Medical what it does is it looks at a medical note and can extract key information. So if you think back to in high school when you used to read a book and highlight in yellow there were key concepts that you wanted to remember for an exam, Amazon Compian Medical same thing it actually can lift key elements and goes from a text blob to discrete data that has relationship ontology and that allows data sharing where you need to. But then there's one other piece so that's when you're allowed to disclose. There's one other piece, sometimes you and I want to work on something but we want to actually redact the patient information that allows data sharing as well. So Amazon Compian Medical also allows you to redact think of when a news channel shows that federally protected document that's blacked out Amazon Compian Medical can also remove patient identifying information so if you and I want to collaborate on a research project you have a set of data that you want to anonymize, de-identify. I have data information I want to de-identify to put it together I can use Amazon Compian Medical redact all the patient information, make it de-identified you can do the same and now we can combine the three of us that information to build models to look at research and to do data sharing. So whether you have full authority to share patient information and use the ontologic portion of it or whether you want to do de-identify matter Amazon Compian Medical helps you do that.  Most people would thank me for that or I can opt out of ride sharing and only take taxis for example but we are all sooner or later going to be customers of the healthcare industry and as a result this is something that affects all of us whether we want to acknowledge that or not I mean some of us are still young enough to believe that we have this immortality streak going on so far so good but as it becomes clear that this is the sort of thing where the ultimate customer is all of us as you take a look at that does that inform how AWS is approaching this entire sector? Absolutely in fact I'd like to think that AWS brought a physician to lead this sector because they understood that in addition to our customer obsession that we see through the customer to the individual and that we want to elevate the human condition we want to obsess over our customer's success so that we can affect positive action on the lives of individuals everywhere. To me that is certainly the reason I joined AWS and so that's certainly the practice of healthcare life science and genomics at AWS has been around for about six years AWS you know that's about double that and so actually it's a mature practice and our understanding of our customers definitely includes that core flame that it's about people and each of us come with a special story and in fact you know the people that work in the AWS healthcare life science team there are people that have been at the bedside there are people that have been at the bench that either worked in the farm industry healthcare population health they all are there because of that thing you just said certainly I'm there because of that and the entire practice of AWS healthcare life sciences is keenly aware of looking through the customers to the individual. All right how about genomics, you know definitely an area where compute and storage are critically important and we've seen dramatic change you know in the last five to 10 years anything specific you could share on that? Genomics is an area where you need incredible compute and storage and in our case for example Illumina which is one of our customers runs about 85% of all gene sequencing on the planet is an AWS customer and stores all that data on AWS so when you look at genomics the real power of genomics is the fact that enables precision diagnostics and so when you look at for example one of our customers Grail Grail that uses genomic fragments in the blood that may be coming from cancer and actually sequences that fragment and then on AWS we'll use the power of the compute to do machine learning on that genomic sequence fragment to determine if you might have one of those 10 to 12 cancers that they're currently screening for and so when you talk about precision health it really can't be done without precision diagnostics which depends on genomics which Grail is an example of that it runs on AWS because we bring compute and storage essentially infinite power to do that I'll give you one more example the first whole genome sequence took 14 years and how many billions of dollars Children's Hospital Philadelphia now does a thousand whole genome sequences in two hours and 20 minutes on AWS they spike up 20,000 CPU cores to do that in Desi and then move it back down genomics the field that literally can't be in my humble opinion can't be done outside the cloud it's just the mechanics of needing the storage and compute power is one that is born in the cloud and AWS has those examples that I shared with you yeah it's absolutely a fantastic and emerging space and it's interesting to watch that despite the fact that there is a regulatory burden that I don't think anyone's going to dispute that and I guess the gravity of what it does I'm not left with the sense that feature enhancement and development and velocity of releases is slower somehow in healthcare than it is across the entire rest of the stack is that an accurate assessment or is there a bit of a drag effect on that? And do you mean in the healthcare sort of customers or on AWS? I would speak on AWS aside specifically customers are going to be customers love them we do but AWS you know we obviously innovation is our DNA we release gosh you think back 2011 we released 80 product services and features then jumped 2015 where it was like 722 jumped to 2018 where it was 1957 features that's like a 25 fold our pace of innovation is not going to slow down it's going to continue it's in our DNA we in fact hire people that are just not satisfied with the status quo and want to innovate and change things just you know innovation is the beginning not the end of the story so no I don't anticipate any slowdown in fact when you add machine learning models and machine learning services that we're putting in I only see an even faster hockey stick of the services that we're going to bring out and I invite you to come to re-invent where we're going to announce them all and you'll be able to be there and see that. All right well on that note thank you so much for giving us the update on healthcare life sciences and genomics absolutely want to see the continued you know growth and innovation in that field. My pleasure thank you for having me on the show. All right for Corey Quinn I'm Stu Miniman the cubes coverage never stops either we of course will be at AWS re-invent this fall as well as many other shows so as always thanks for watching the queue.


In materials and instruments, we need different reagents like lysis buffer, proteinase K enzyme, sodium duty cell sulphate, ethanol and isopropanol. In instruments, we need pipettes, pipertips, vortex, centrifuge machine and deep freezers to store the DNA. Now in the basic steps of DNA extraction, there are six core steps involved. The first step is the cell lysis. Second step is the digestion. Third is the phase separation. Fourth step is the DNA precipitation. Fifth step is the washing by the ethanol and then air drying and the storage of the DNA. First of all, we will take the sample to extract the DNA and the sample that we have is the human blood sample. We will take 200 microlitre of, we will take 200 microlitre of human blood and will add in each tube. After the addition of 200 microlitre blood sample, we will add 1000 microlitre of lysis buffer. So we will pick the pipette which is 100 to 1000 microlitre and will set on 1000 microlitre. Now we will add the 1000 microlitre lysis buffer in each sample. After the addition of 1000 microlitre lysis buffer, we will vortex the sample for 5 minutes. This is vortex machine and we will now vortex the sample to homogenize the mixture. After the vortex, we will centrifuge the sample at 13,000 rpm for 15 minutes at 4 degree Celsius. We will keep the samples in centrifuge machine. It should be remembered that while keeping the samples in centrifuge machine, centrifuge machine should be balanced from each side. Centrifugation will be done at 13,000 rpm for 15 minutes at 4 degree Celsius. So we will set the centrifuge machine at 13,000 rpm at 4 degree Celsius for 15 minutes and start. After the first washing, we will discard the supernatant and at the end of the tube, we have a pallet which contains our DNA. Pallet is actually a collection of cells where the DNA have and after these steps, we will go for the cell lysis through the digestion buffers. This was our first washing. We will discard the supernatant preventing the pallet. Then microlitre of lysis buffer and we will again do washing until the pallet become colorless. For the second washing, we will again add the 1000 microlitre lysis buffer. After the addition of 1000 microlitre lysis buffer, we will again move to centrifuge machine and we will centrifuge the samples for 15 minutes at 13,000 rpm at 4 degree Celsius. After the second washing, the results will be like this. You can see the pallet has become somewhat colorless but we will again do the third washing until the pallet will become totally colorless. We will discard the supernatant preventing the pallet. Again, we will add 1000 microlitre lysis buffer for third washing. Third washing, we will centrifuge the sample on the same conditions as previous like 13,000 rpm speed at 4 degree Celsius for 15 minutes. After third washing, the results will be like this. The pallet will become colorless. We will discard the supernatant preventing the pallet. After the three steps of washing, when the pallet will become colorless, we will add 20 microlitre of Proteinase K. The basic function of the Proteinase K enzyme is to digest the proteins. As we know, the DNA is associated with histone proteins for its packaging inside the nucleus of the cell. So the Proteinase K will digest the proteins which will lead to the specific extraction of DNA. We will took that pipette within a range of 20 microlitre in order to add the Proteinase K in the solution. After the addition of Proteinase K, we will add the 80 microlitre of sodium rhodosyl sulfate which is commonly known as SDS. The basic function of SDS is also to denature the proteins. Basically, SDS is a detergent that imparts negative charge to all proteins. And we know that DNA also contains the negative charge. So when all the proteins get the negative charge, they will get repelled from the DNA and will be separated from the DNA. So SDS is also used to digest the proteins. We will take the pipette within a range of 10 to 100 microlitre in order to add the sodium rhodosyl sulfate. We will set the pipette at 80 microlitre. After the addition of 80 microlitre SDS, we will add 250 microlitre of buffer A1 which will be used to stabilize the DNA at the required pH. We will take pipette of range 100 to 1000 microlitre and will set on 250 microlitre in order to add the buffer A1 in the solution. After the vortex, we will incubate the samples at 58 degree Celsius for overnight digestion in water bath. The basic purpose of overnight digestion is to obtain the DNA purified from the proteins. So the proteins will get denatured and digested overnight with the help of SDS, Proteinase K and the buffer A1. We will keep the sample for overnight digestion in the water bath at 58 degree Celsius. Water level should be enough that our sample should be dissolved from the bottom.