وَأَقُولُ فِي القُرْآنِ مَا جَاءَتْ بِهِ آيَاتُوهُ فَهُوَ الْكَارِيمُ الْمُنْزَالُوَ وَأَقُولُ قَالَ اللَّهُ جَلَّ جَلَالُوهُ وَالْمُصْطَافَ الْهَاديِ وَلَا أَتْ أَوْوَالُوَ الحمد لله رب العالمين له الحمد الحسن والثلاء الجميل واشد أن لا إله إلا الله وحده لا شريك له يقول الحق وهو يهدي السبيل واشد أن محمد العبد هو رسوله سلالله وعليه وعلى آله وأصحابه والتابعين لهم بحسان إلى يوم الدين أما بعد The introduction that I want to speak about today is التجمول للناس Beautify yourself for the people وتركوا التجمول للله في الصلاة and leaving off beautifying yourself for Allah when you pray As we know Allah is beautiful إن الله جميل يحب الجمال Allah is beautiful and he loves beauty سبحانه وتعالى many of us we contradict ourselves We wake up and we pray Fajr and we pray Fajr in our pajamas right now we stand up and we pray our Fajr in our pajamas and when we leave the house though and we want to meet friends or we want to meet our colleagues at work we make sure we choose our clothing we sisters put their makeup they get ready they leave when the salah like in his coffee here throw the hijab on just pray don't worry you're standing in front of Allah سبحانه وتعالى why are you contradicting yourself when you stand in front of the human beings you want to look a certain way your conscious of your dressing brothers and sisters but when you stand in front of Allah you're not didn't Allah not say يا بني آدم اخوضوا زيناتكم عند كل مسجد take your beauty with you in every prayer شايقو الإسلام بمنوتيم he said in his مجموع الفتاة the 22nd volume in page 117 he said فتقضو زيناتكم لمناجاتي سبحانه you should take your beauty your adornment when you are going to meet Allah سبحانه وتعالى when you're going to talk to him you know the prayer is a dialogue and a discussion between you and Allah ولي ذلك ابن عمرن he said ابنوتيم he entered upon his غولان his slave boy نافع and he said to him after he saw him praying with his head was not covered and of course that for them was a sort of he wasn't a from their norms and their customs is that the person whose hair is حاسرا whose hair is uncovered is a form of you're not respecting that person that's the norms that they had and even in the Arab world today it's like that if you haven't got something on your head generally they see it as a rude thing so ابن عمرن he saw he nafee praying the صلاح حاسرا ابنوتيم he says that he said to him when ابن عمرن saw him he said if you left for the people what would you do? if you were going out and now you're going to meet the people when you have covered your head he said to him كنت تخرجوا هكذا would you leave it like this? نافع said no ابن عمرن he said فالله أحقوا أن يتجمل له فالله الله is أحقوا من يتجمل له الله is the one who is the most deserving to be beautified for and of course this is the حديث of the the Prophet ﷺ where he said فإن الله أحقوا من يزيّن له الله is the one who has the most rights for us to beautify ourselves for him so you're contradicting yourself and we all are contradicting ourselves when you pray the Salah in the most scuffiest clothing but when we meet a human ضعيف a human being we dress ourselves up we look a certain way we put perfume on but when we stand in front of Allah we look so unappealing I'm going to stop there إن شاء الله anything which I have said that was wrong incorrect is from me أشياء ضعان and Allah his messenger are both free from it سبحانك اللهم سلام عليكم ورحمة الله وبركاته كيف can you do a two second action right now that will give you a share of the reward of everything we're doing on this YouTube channel simple like this video and click subscribe why it will allow YouTube to recommend our videos to other users and imagine the huge amount of reward that could be waiting for you on the day of judgment if you did that with a sincere intention of spreading the Dean of Allah you'll be rewarded for every single person who benefits from one of our videos as a result of your like or subscribe that's an easy two second action that you definitely don't want to miss out on do it now click like and subscribe and don't forget to make that intention


a surprisingly downbeat forecast for the 2020 housing market from one of the largest names in real estate and it could have big implications for consumer spending. Diana Ola joins us right now with more Diana. Good morning. Good morning. Becky realtor.com one of the nation's largest home listing site says the housing market will slow down next year. The headline of the report is home sellers will remain on the sidelines and that will cause the inventory shortage to get even worse and could even hit that record low. Now low inventory will cause sales to drop just under 2% from this year according to the report and home prices will flatten nationally as millennials who account for half the buying market struggle to afford overheated prices in some major markets. However, realtor.com is predicting prices will actually go negative. The first time that's happened since the housing crash. Now those cities include Chicago, Dallas, Las Vegas, Miami, Detroit, St. Louis and San Francisco with prices weaker sellers will have little incentive to put their homes on the market. Now home construction is increasing, but it's largely on the upper end of the market, not at the entry level where demand is strongest. One of the only bright spots in the report is that mortgage rates will remain low. And in fact, they moved sharply lower yesterday on the China trade news. Okay, what's up everybody? Ricky Kruth here. Welcome back to my channel. So that was CNBC reporting that realtor.com was calling for a market downturn in 2020. They're calling for one point eight percent less transactions next year. They're also calling for their average prices to flatten out and even in major cities for prices to decline. So what in the world does this mean for real estate agents and real estate investors? Absolutely nothing. It's good to know the market trends. It's good to know where we are in the market cycles. What we need to realize is that there's always, always an opportunity. Let's keep in mind a few things here. Number one, always like and comment on every single one of my videos. Something else that we should remember is that closings continue to happen every single day, regardless of what the market does. The market can go up, down, sideways. It doesn't matter. Closings are going to continue to happen every single day. And every closing represents two opportunities, a buyer and a seller that actually went through with a transaction and closed a transaction. So this news isn't really going to affect us very much. If anything, it's going to excite us because it's going to show us that there's going to be some opportunities next year. Why? Because the mainstream of the industry, mainstream agents, mainstream people out there watching the market, they're going to view this as a negative and they're going to start to pull back. They're going to start to retract. They're going to start to slow down. They're going to start to tell theirself that business is going to slow down. So I need to start preparing for this slowdown. Whereas the zero to diamond real estate agents like myself and you that are watching, we're going to go towards the downturn. We're going to have an even stronger commitment in the industry because we know that this is where we're going to what we're going to acquire more market share. I'm going to link a video below about the shortage of business during a market downturn that I really think you need to watch. Let me tell you what happens during a market downturn. Okay, let me tell you about what happens from the point of view of the real estate agent and how we can thrive through this and also a real estate investor and how we can thrive through this. Now, as a real estate agent, it doesn't matter if you get in the business the very day that the market crashes the hardest. Okay, it doesn't matter. Closings continue to happen. What is our job? Our job is to get out there in the market, talk to as many people as possible with the best intentions and build our brand. Okay, through the market crash, a couple of things happen. This is very interesting. As the market crashes, okay, this is when your big time investors come out of the woodworks because they want to buy when it's cheap. That's even more extremely relevant in today's market than probably any time before because we just experienced the big crash just 10 years ago. Okay, so the big crash happened a decade ago and now we're still kind of people still have a sour taste in their mouth about how that affected them. Okay, so they know another crash is coming. So there's a lot of cash on the sidelines waiting on the next crash to happen so they can take advantage of cheaper prices. They're not going to let what happened to them in the last crash happened to them in this crash. So there's a lot. I repeat a lot of people buyers investors sitting there waiting for prices to come down. And if we're the agent that understands this and we're out in the market talking to people seeing what we can do to help them, then we're going to find these people that want to invest at cheaper prices. Now, where do we find these people that want to invest at cheaper prices? Where are they? Well, for one, they own property already. Okay, they live in houses already. So if we're doing what I teach in my zero to diamond free real estate coaching program, we're going to be circle prospecting calling expires or sell by owners, whatever you're doing, we're going to be reaching out to these property owners and we're going to say, Hey, the market's crashing. Prices are this house did just sell in your area. Didn't if there's anything you could do for you, do you want to buy, sell or hold? That's the three options for a property owner during a market downturn. They're either going to buy because prices are cheap and they want to buy right now before prices go up. Okay, I feel like when the good deals come through, they're going to have multiple offers on them during the market crash. Okay, I'm kind of predicting this. I think as the market downturns and we're going to see lower prices, I think we're going to see multiple offers during the downturn because there's going to be so many investors sitting here with cash that want to buy these good deals. So there's going to be a lot of urgency there for people wanting to buy at cheaper prices. Okay, then we have the people that want to that need to sell because they're in trouble. Okay, something went wrong. The the crash caught them somehow financially. They didn't see it coming and they're in a position where they need to sell. Okay, so now we're going to help them sell. What do they need to do? How quickly do they need to sell it? Right now because they're in trouble. So they're again, urgency. So we've got buyers that want to buy now, sellers that got to sell now. Okay, then you have people that just want to hold. They want to ride the market out, see where it's going to go. They're not in trouble. They don't really have any cash to invest or don't want to invest and they just want to kind of see where it goes. They want to be very conservative and that's fine too because when the market rebounds or if something significant happens in their life that causes them to need to buy or sell, we're going to be there for them. So even the people that want to hold that don't want to buy or sell anything while the market's down or right when you talk to them, that's okay. We're going to ask them if they have an agent they would work with, if they were to do something and then we're going to ask them if it's okay to stay in touch. The whole nine yards, the script. Okay, the Ricky Crews zero to diamond script. When the world can I do for you? Is an agent you would work with? I'd love the opportunity to stay in touch with you and work with you one day. We're going to grab their cell number and their email address. We're going to do weekly emails and I'm soon going to release a mass texting platform. We're also going to text that weekly email link. We're going to text that link as well to our clients. So that's where you're going to find the people that want to buy, sell, or hold. Alright, and I'm telling you that there's going to be so much opportunity. It's going to be unreal. If you're going to stay on the phone and continue contacting people and be that agent that's out there making contact, helping people through this hard time, then you're going to be the one that not only thrives but when the market rebounds, you're going to have acquired so much market share that your business is going to literally explode. Now let's talk about the investing side. If you're a real estate investor, what are we going to do? How are we going to prepare for this? What are we going to do to take advantage of this opportunity? So first off, your real estate portfolio should be set up for long term. Which means that everything that you have in your real estate portfolio can't really get hurt in a market downturn. There's really no risk involved. That's how my real estate portfolio is set up. For example, all my long term rentals, I have mortgages on them. The rent pays the mortgage plus. Okay, so I have cash flow there on all those properties. If the market does downturn, nothing's going to affect that. Rent may even go up during a downturn if people are getting their houses foreclosed on. So it's a very safe bet to have mortgages on those properties. The rent is paying the mortgage off and I have cash flow out of it that I can put back into the properties to further the appreciation and equity. Now if you're fixing and flipping, if you're buying properties to fix them up and flip them immediately or if you're just buying them to not even fix them up, just flip them if you're finding good deals. If you have some kind of angle, some strategy to get really good prices on property that you can turn around and sell for higher prices. Okay, this is where I don't want to over leverage myself at all. So I play it really safe and I don't borrow any money on my fix and flips. I pay all cash for my fix and flips because my fix and flips are short-term investments. So I don't want to borrow money on short-term investments because something could happen. I'm not expecting short-term that will create a situation where I may get in trouble. Okay, so that would be highly unlikely, especially where I'm right now financially, but I still want to play it super conservative. Okay, so I have partners, we go in, we buy these properties one a month or so, on average I would say, fix them up, flip them, and get them gone. As we're fixing and flipping, we try not to own too much at once because if we own too much at once and the market crashes, then we own all this property and we could get hurt if prices go down far enough. But if we only have a handful of houses, you know, five houses, something like that and the market comes down some, we can sell all those for maybe a little bit of a loss or break even or maybe just make a little bit, we can get out of those quickly and then kind of regroup and know that, okay, we're in a different market now, we need to buy at much cheaper prices than we were buying. And you see how that works, how you can't really get hurt if you play it like that. So the market has been changing for about 18 months now or maybe longer. Ever since interest rates started to creep up, then they creep down. Okay, so and I think it's a good thing. I think if the market would have just kept going up and up and up and up, we'd probably be in more trouble right now than we are. So I think that that's a good thing. I'm glad it kind of flattened out and settled out. That was a good thing. And if next year, realtor.com is correct and prices kind of decline, flatten out, we have less transactions, I say it's time to fully commit to the industry, whether you're an investor or a real estate agent, I think it's time to go full force. Right now, December, right this second, I think you should be going full force to build your database. Find those people, build those relationships. Because if things do turn around, let's think about the two different scenarios. If things turn around, you're going to have all these relationships built you can reach back out to and say, hey, if the prices are down, do you want to buy, sell or hold? Okay, or if the market increases, let's say they're wrong in the market increases, you still have all these people you can you can reach out to and say, hey, do you want to buy, sell or hold? It's really the only three options, whether the market is going really great or whether the market is going really bad. Buy, sell or hold. Anyway, I don't want any of this news or media about the market flattening out or turning down or less transactions to scare you. I wanted to excite you. That's wanted to make this video and just make it clear that this is not anything to be afraid of. This is something you should run towards. I want to give you another video right now that I think will really help you with your mindset and that's three real estate mindsets that I made about a year ago. I'm going to link it below along with the shortage of real estate business during a market crash. I'm going to link both of those videos below and also we're doing the zero diamond talk show every Tuesday, 4 p.m. Central. It's live right here on my YouTube channel. My co-host Joe Rosen and we have a lot of fun and we take your calls. So we have a really good subject every week. Sometimes we have a guest and we take your calls. I want to hear your questions. It's designed so that you guys can call in and ask questions and I can help you through some of your specific problems and questions. I'm also answering every single DM on Instagram still. So if you want to reach out to me there, feel free. And if you haven't joined my absolutely free real estate coaching program, that's at zero to diamond.com. We're making so much progress in the industry. I have thousands and thousands of messages from you guys telling me all your little successes and things that that zero to diamond has done for your business and your life. And so I'm just very humbled and happy to be a part of it. Anyway, that's it for today. Thank you guys so much for watching. I'll see you on the next video. Let's go!


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How to use retargeting to make more sales, hey it's a fact 98% of the people that come to your website, your landing page, your sales page are not going to buy. That's not good. Now here's another fact, probably 90% of those people are going to buy at some point. So how do you sell to them? I mean if you're not selling those people and they're leaving your website, you're leaving huge, huge sums of money on the table. How do you fix it with retargeting? What's retargeting? I'm going to show you what retargeting is and how you can use it to make more money today and we're starting right now. Hey, I'm JR Fisher. Welcome to my channel. If you're brand new, we want you part of the family. Here's how you can become part of the family. Go down there and click that subscribe button and if you want to be a real VIP, smash that notified bell. Yeah, smash that bell and turn on all notifications and guess what?  I've been selling online since 2009 and I've sold millions of dollars, I guess so now, of actual physical products and digital products and on this channel, I teach you the tricks and tips that I figured out along the way and the things not to do so that you can get good results. Also, as a bonus at the end of this video, I'm going to give you access to a $97 course, but it's not going to cost you anything. It's going to be absolutely free and I'll tell you how to get that at the end of this video. All right, so let's get right on it. What is this retargeting thing? Okay, you want more sales? Of course you do. Everybody wants more sales. Everybody wants more sales, but the question is how do you get them? I mean, I know we have our advertising in place and we're running ads and we're getting people, but what if you could get your conversions up? In other words, what if you could get a higher number of those people that you already paid for to buy your product? That's what we're talking about when we talk retargeting. Now the question is, are your advertisements really reaching an actionable audience? Now, here's the thing. The major difference in just advertising and retargeting is retargeting, we actually are going after an actionable audience. And what's an actionable audience? Well, these are people that may have already interacted with you. Maybe they went to your website. Maybe they went to your sales page. Maybe they opted in for something you had. So these people had some type of connection with you and that's the people we want to retarget. Now, while it would be a great scenario to be able to run an ad and have people buy your product and that's the end of the story, it's not that simple. It's not that direct of a path. Sometimes people need more information. They need to be pushed a little bit. They need some bonuses maybe. They need to be reminded maybe. There's a lot of reasons for running retargeting campaigns. Okay, now here's a visual representation of how consumers interact with a brand before, during, and after they make a purchase. Now, take a look at all those touch point examples in the awareness and consideration stages. It shows that the buying process isn't necessarily a straight line. A consumer may stumble upon your website one day, browse it a bit and then just leave. And maybe a month or two later, they read one of your blog posts and they subscribe to your email list and boom, they're back again. And then, you know what, maybe they could call your business. They could talk to one of your sales people. They could talk to an operator and then they have a little bit more information. Maybe they go back to your website again. It's not a straight line. As a marketer, it's our responsibility to understand that process and understand that it's not a straight line. If we do that, we're going to be a whole lot more successful and we'll make more sales. Now, what I'd like to do is actually explain everything you need to know about retargeting so you can use this really cool tool. Now, make sure that your retargeting strategies actually have a goal, OK? Some people don't even have a goal for it. First, I want to cover all the basics. Now, retargeting ads are for people who have specifically been to your website or had some contact with your company. They already know something about you, OK? They are maybe lead generation type of ads or maybe they were download PDFs or something, but they had some contact with your business. Or maybe they just visited your web page and didn't do any action at all. The ads for these type of people are not as simple as banner ads that you would just use to target anybody. There are seven different types of retargeting methods and I want to go over each one with you. Now, with so many different ways to approach this marketing tactic, it's important to have a clear goal in mind. If you've ever done this before, I don't recommend you trying every single one of these techniques. Just pick one that you really like and just go with that one. All right, here's some examples of ones you can do. You can target people who have Google searches, right, that are relevant to your actual product. That's one way you can do a retargeting campaign. You can also target people who've already consumed some of your content on your website. So those are maybe pre-customers or they could be regular customers right now too. It could also be people who visited your website and didn't make a purchase, but it could also be people who are on your email list, which could be buyers and non-buyers. Now, knowing who you're gonna retarget, well, that makes it all the easier, but you've gotta have a really good understanding of where these customers are coming from. That's so, so important. If you have that, then you can set up your retargeting campaign and you'll get results. The goal of a retargeting campaign in general is really just to create more awareness and then eventually get more conversions. As you saw earlier, people aren't always ready to buy, you know, the first time they land on your site. Sometimes they need a little nudging. Sometimes they need to gather more information. Sometimes they need a little bit more time. There's many reasons why they don't buy the first time around. Here's something interesting that you probably don't even understand, but 92% of the people that come to your website, they aren't looking to make a purchase. They were just doing some research. People will often do a lot of research before they actually buy a product. And if you go into it thinking they were buyers in the beginning, you're missing the boat because what they really need is probably a little bit more engagement and a little bit more education about your product to push them over the edge to make them buyers. Now, what your goals are could be varied. You know, it could be driving customers to certain products. It could be getting them to subscribe, getting them to sign up for different services. But you got to decide that upfront because we got to know what those goals are. Just make those goals really clearly defined in the beginning and you'll be successful. By doing that, we get everybody on the same page and your odds of success are so much better. Pixel-based targeting is one of the most common ways to retarget people who've been to your site. Now, on the screen right now, here's an example of how DoHOP implements this particular method. Once somebody visits your website, a browser cookie is stored to retain that information. This is how you're able to find people who have visited your website. They're kind of like marked. Now you need to get a retargeting platform. The cookie will notify the platform and provide that consumer with your ads based on what pages they viewed on your website. Now, pixel-based targeting is great because of timing. What happens is once they visited your website, that pixel is put on them and they will almost immediately start seeing your ads as soon as they leave your website, which keeps your company in the front of their mind. But keep in mind, if you're getting no traffic at all right now, you can't use pixel-based retargeting because nobody's coming to your website. Now, list-based retargeting focuses on people who are already on your list. Now, it's not as common as pixel-based retargeting. However, it works really well. You can even focus on your existing customers. Those are great customers to sell another product to. Once somebody's bought from you, they're a whole lot more likely to buy again. You can segment these people into different groups and run different ads based on what segment of the audience they're in. Don't forget to update your ads. One mistake I see companies do all the time is they run the same ads over and over and over. They're not gonna convert 100% of the time. You have to change up your ads. You have to change up your images. You have to change up your copy. It's a constant thing. It's not something you do once, figure out a great ad and run that for years. It's just not gonna work that way.   They don't think it's a coincidence anymore once they've visited your site that all of a sudden one of your ads pop up, okay? So we have to be a little bit more creative than we used to be because they're a whole lot more educated. So what you wanna do is switch it up. If one of your ads is not converting with this group after a week or two, switch it up. Do a different ad. Come out with a different creative. Come out with a different text, a different offer. Something that's fresher because if you keep running the same thing over and over, they're gonna get ad fatigued. They can actually go in and turn off your ads to where they won't see them at all anymore. Now, here's an example right here. Take a look at this retargeting from Fresh Desk. It's simple and has a clear goal. Look at the CTA, the call to action button there. You see that? They are trying to entice the customer to download something they offer. Now, this could be targeted towards people who visited a specific landing page on their website or some other area. This ad is corresponding to a browsing cookie for that particular page. But what happens after someone sees this ad for a few weeks without converting? Will it eventually work if you just keep showing it to them? I mean, after a while, will you just beat them down? Probably not.  They probably won't buy, okay? So that's why you need to break it up and do something different. There are major differences between this ad and the first one above. Rather than getting the customer to download something, they are trying to encourage them to sign up for a free trial of their software. Different offer. Offering them something different can potentially increase the chances of getting a conversion. What you're gonna need to do is you're gonna need to A-B split test this.  They could do a lot of things. I mean, they could change the color of their ad. They could move the call to action button. They could change what the button says. But the point is, they need to do something different or it's gonna get stale. The next thing is, by doing this, A-B testing ensures that your funnel is gonna be optimized at each stage. Now, the next thing you can do is create custom landing pages so that depending on which ad they clicked on, they're gonna hit a different landing page that's gonna be different in offer and color and message, whatever it is, but it's gonna be a completely different landing page. Now, here's an example of an ad I saw on Facebook. Now, it's obvious that this particular ad is focused on men because it says men on it. Okay, so they're obviously going after men, but if you go to the Lulu Lemon homepage, it's primarily directed towards women, which is entirely different. Now, sending customers here would be ineffective for that Facebook campaign. Lulu Lemon recognizes this so they don't do it. Okay, it would make sense. Instead, Facebook ads go to a customized landing page designed specifically for men. Here's what happened when I decided to click on the retargeting ad. Okay, so this page speaks to me more than the homepage. It's also directly related to the Facebook ad. They are promoting discounted men's clothing. Now, apply this concept to your retargeting strategy also. If you're pitching a specific product or service, make sure the landing page is like the ad. You see, the point is if you just send them to your webpage and they have to search your website to find that product, you're gonna lose a lot of sales. You don't wanna do that. What we're trying to do here is we're trying to focus on our existing customers. Your retargeting campaigns do not have to draw in new buyers. We can actually retarget people who already bought and just increase our customer value by doing that.  And when I say segment it, we wanna look at people who did buy and people who didn't buy because the ads we're gonna send to people who did buy versus the ads we're gonna send to people who didn't buy are gonna be different. Now, the benefit of retargeting your current and old customers is that they already have this level of familiarity with your company. They know you, they know your brand, and they're actually a little bit better to sell because they're easier to sell. They already have confidence in you. The more they know about your company, the more likely they're gonna be to actually buy the product. You can also do upsells and downsells and cross-sells with these people because they could be a different type of product from what they already bought, but it could just be related. Here's a great example of what I saw from American Express. Now, my wife is already a member of American Express and has one of their credit cards, but they are trying to pitch a new card to me. Rather than getting a new customer, it's easy for them to retarget me by offering me a card I don't even have. Now, of course, if this doesn't work and I don't get their card, I could potentially see an entirely different ad in a month or two from now. Now, the audience and target market for these ads should really be the type of people that are ready to act right now. That's why retargeting campaigns work so well. What you're doing is you're actually focusing all your advertising dollars on people who are already familiar with you and your brand and maybe your products. Now, if you're using the pixel-based retargeting, what you're doing there is you're targeting people who have visited your site but not necessarily converted yet. Now, you would think increasing the traffic to your site would increase your conversions, but it may not be the case. It may be that people need to get more information to buy your product. So just sending more people to your site constantly may just drive up the number of people that visited your site, not necessarily get your buyers. But if you go after those people who have visited your site and they already have some knowledge of your company, that could actually drive up your sales a whole lot better. Make sure you have a clear goal for your campaign. You know, you're gonna do several ads and you could do an ad that just doesn't convert. But don't give up on those customers. You know, after a week or a month or whatever, if they haven't bought, switch it up and do a different type of retargeting ad, then maybe that one will work for you. The problem is most people will try one thing and they just quit and you need to try several things because you don't know what's gonna work, but one of them will work if you try enough. Now, I gotta tell you this. When they click on your ad 100%, make sure they don't go to your own page. I know I did this. I was spending thousands of dollars years ago and I was sending everybody to my homepage and they would get there and they had nothing to do. So when you're running an ad, you wanna run an ad for a specific product and they need to go to a specific page that just offers that product. Not a wide variety of all your products and I know the thinking is, well, if I have a lot of products, then the better chance they're gonna buy, nothing can be further from the truth. You need to advertise a single item and it needs to go to that page with that single item on it to sell it. Now, if you follow these steps and you use retargeting correctly, you will increase your conversion. It's been proven, many companies do it, it does work. If it's not working for you, you need to go back, watch this video and make some changes that I talked about throughout the entire video. Okay, so you've got a game plan. You can do this. You can use retargeting. If you liked this video, give me a thumbs up, right? Don't forget to subscribe, that button down there and turn on notifications. Don't forget to click all notifications. That way you become a VIP member of the Fisher family and I can notify you every single time I do a new video. Now, I've got a course. It's an e-commerce course. It's $97, but you don't want to pay anything for it. You can get it for free. There's a link right below in the description. Click that link. No credit card required and you can start learning today. Thanks so much for watching this video and I'll see you in the next one. Hey, thanks for watching my video. Don't forget to subscribe to my channel and click that little bell right here so you can be notified every time I do a new video. Also, click on one of those videos there. Keep watching on my channel.


Welcome traders to another Tick-Mill Chart It with me, Patrick Munnally. I want to draw your attention today to a developing opportunity in the Kiwi Dollar, potential 10-shoulders pattern developing here versus the cycle highs at 67.90 area. We've seen the decline here, potential neckline developing, trading back up into the monthly GWAP, got a rejection from there yesterday from the monthly pivot and now trading below the weekly pivot, consolidating overnight. So what I'm looking for is some follow-through here through the 65.70 level during the London session, initially targeting obviously the neckline at 65 then on to the equality objective at 63.78 and if we can get through there then the head and shoulders objective is actually back down at 62. As always traders, plan the trade, trade the plan but most importantly manage your risk. Until next time, thanks very much.


First of all, let's start with the documentation. As usual, please follow the link in this slide to get full access to all of the documentation both by Microsoft, as usual docs.microsoft.com slash Azure slash Netflix Duo and so on, and also the documentation by ST in terms of Wiki. So Wiki.ST.com slash Netflix Duo, et cetera. On the XQ Badura toss that we are using since this early morning, inside this package, we find also the source code and the example for the Netflix Duo. So not only the operating system, the file X, the USB X, but also the example for the network Duo for the TCPIP. Let's go inside more detailed. The Netflix Duo is IPv6 already certified. This is because of the naming Duo, IPv6 plus IPv4. It's supporting a lot of LFC, both for IPv4 and IPv6. All of them are certified by XIA, by EXANVL, so Automated Network Validation Library. This is for the compliance to all of the RFC listed here. The source code is provided again in C code. The list of the API is BSD compatible. So for the ones that are already familiar with the Unix system, the Linux system, you have again also here the API in BSD format. So you have the socket usage and bind list and accept. So the usual API. Chart about the security. When we talk about Internet of Things, it's always a matter of security. The security is a big part inside this library. You have security across all of the TCPIP layers. From the IP with the IPsec going up to the TCP and UDP with the TLS and the DTLS, and also the legacy version of the TLS 1.0 that is the SSL 3.0. TLS is supported up to version 1.3, the latest release in 2018. This is a plus in terms of security, respect to the 1.2, the current standard of the FACTO. It decreases the number of message exchanged during the end shake from 4 to 2. This means that using the TLS 1.3, it's decreasing the potential vulnerabilities. Let's say, if you half the number of message exchanged during a connection, you are also helping the number of potential vulnerabilities, the exposure to the potential vulnerabilities. Obviously, with the new standard, TLS 1.3, more security features are added, more elective curve, and not only because it also stops all the protocol, like the CBC, like the RC4, like the Shawan, like the MD5, and so on. All of the protocol that are no more considered valid and strong to the potential attacks. I want to highlight also the DRFC 52.8, so the support of the certificate in PGAI format, both in an ashy way and in a binary way. Going on about the certification, like we did in the previous session about the FileX, also here you will have a lot of certification by Tuve and UL for the electronic device, for the medical device, for the automotive industry, for the railway industry, and also for sure about the UL, about the home appliances, so all of the electricity, gas, and so on devices. All of them used at home. Not only designed for security, but also designed for small sites because it's based on Peconet architecture, so written for embedded system. It's scalable, it's written in C code, it's highly modular because its functionality is included in a separated C file, and it means that it's more easy to update and employing hardware resources of the MCU. It's highly scalable because a linker is absolutely not adding any feature that is not required by you, but your code. Like FileX and USB-CX, also the Netflix Duo is supporting the Trey6, so you can perform some post-mortem analysis to try to understand what is succeeding or what is succeeding when you stop the debugger. Being part of the Microsoft Suite, it supports also the FileX. Since you, for example, can embed some server inside your device, some web server, for example, you need a file system to search for the required resource, for the requested resource, and give back to the client that it's asking. So you need a file system, and the FileX is fully supported. In the demo we will see later, FileX is not included to offer, to give you a more easy to understand, a more smaller demo. We will use a file system that is directly written in flash. So the HTML file, the resource file, the JPEG, PNG, and so on, are converted into binary format, so into another file, and written inside the flash of the microcontroller. But this is not meaning that the Netflix Duo is not supporting FileX. This is only a way to simplify the demo. Chart about the list of the protocol supported. This slide is not fully including all of the protocol that are offered by Netflix Duo TCP IP stack. For the full list, I kindly ask you to follow the link in the top right corner to have a full list of all of the supported protocol. Let's quickly scroll the table. For example, let's start from the beginning, out of P. Out of P is a way to automatically assign an IP address to the device if the device is not able to get an IP address from the network. For example, because the DHCP server is not replying. The device, the stack is able to automatically assign itself an IP address in the range of the <IP_ADDRESS> with 16-bin network. And this IP address is only used as a recovery mechanism. Because when the DHCP server will come back to life, let's say the out of P will stop and the real IP address will be collected by the server and use it. DHCP server client is supported. You can have also a column about the sides needed, both in terms of flash usage and RAM usage here. ICMP and EGMP are supported. So control message protocol versus group message protocol. So unicast versus multicast and so reachability, I mean the ping versus the synchronization. It's supporting RARP and RARP. It means IP to Mac and Mac to IP. So the physical, the conversion between the physical address and the IP address. NAT network address is a transition is supported. This allow your device, for example, to modify the IP address if you want to implement a kind of a router, let's say. SNTP is supported. SNTP is a simplified version of the NTP being based on the UDP. It's used, for example, by Philex. We saw before, for example, to set up the date when a file is going to be created. So you need a timing and the SNTP is here to solve this question. In the right part of this table, there are protocol, let's say, at an higher level. So at level four of the TCP IP stack, so above the TCP and or the UDP. For example, the SNMP is the network management protocol. Simplified, simply simple network management protocol. It's built on top of UDP and it allows to simplify the configuration, the management of your device inside the network. This is the purpose of the SNMP. There is also the PPP that is not listed here, but the PPP is fully support and it is a protocol layer one, let's say, of the TCP IP stack. So behind the TCP and it can be used to set up a point-to-point connection between two devices, between two nodes. The rest of the protocol on the right side are all of them based on TCP and also being based on the TCP, they are also based on TLS. HTTP 1.0 up to 1.1 and the secure version, meaning HTTPS, and supporting all of the method. We will see later in the demo one of the method used by the web server. Email protocols are supported, both for sending, so the SNTP, and for receiving the POP3 as a client, obviously. Both the transfer protocol about the file are available. So the full version, let's say, based on the TCP and the trivial version based on the UDP, the TFTP. Again, both of the methods are implemented. So you can use both the GET and the PUT. So if you want to download a file, you use the GET. If you want to upload a file, you can invoke the PUT. MQTT is a nice protocol. I think you are familiar also with the MQTT and there is a demo also for the H7 family, also about the MQTT. You can implement this demo inside your nuclear platform, nuclear board, you can, for example, attach on top of the nuclear also, annex nuclear with some sensor and try to build a packet, register to contact a broker, for example, Mosquito, create a topic, subscribe to that topic and then use also other device to subscribe to the same topic and receive the messages sent by your nuclear. This is a mechanism that is quite similar to the WhatsApp groups you have on your smartphone. When you enter a group, you are subscribing to a topic MQTT, let's say. When you receive a message, you are receiving a message because someone else made a post to that topic, to that chat group. Next slide, the sign-in for speed. It means that we are able using this library to reach an almost wire throughput. What does it mean? Since this library is supporting an implementing zero-copy API, it means that the buffer are not replicated, are not copied from a layer to another one. So when a layer receive a message, it's not needed to create another buffer, copy the PDU and pass this message, this smaller buffer to the upper layer. Only a buffer is needed, all of the layer are used in the same buffer, so zero-copy is implemented and this way you are able to reach very, very, very high throughput. This is because we write almost wide throughput. The sign-in for speed also because it's supported the hardware acceleration by hardware, obviously by STM32, about the CRC, so the C-Click Redunda check. Multiple interfaces are supporting. It's possible, for example, to have one public address for the usual operation and another private address for operation like debugging reports, statistic and so on. So multiple IP address and also more security because you are not fully opening all of the feature to the rest of the world. About multiple interface, also multi-homing is possible, is implemented, is supported. This way it's possible to, let's say, freeze your application, so build your application, build your source code, fine-tune your application and in the end, decide which physical channel you can use, you want to use. Also, static routing is supported. Even if using static router is not so useful because it's not, let's say, it's not adaptive, it's not adaptive routing. So if it's not fault tolerant, so if a device in the middle of the network crashes, all of the remaining parts of the network is lost and is no more reachable, let's say. One final slide before moving to the demo, to the show. This slide show you the flow, let's say, from the initialization to the application up and running. There is a fundamental step in the middle and if you are familiar with the Linux environment, you know that when you create a network, the last step is to get an IP address. And in this case, your application register a callback and when the callback fire, it means that your application is able to go on and turn on the remaining part of the system. For example, about the web server. Before the web server is turned on, you need an IP address, basically. And the IP address cannot be self-generated. You need a guy in the network external to the nuclear shield, let's say, external to your device that give you an address. This way, your application, your nuclear is waiting for the DHP server to give you the address. When the address is received, the callback fires and this way your application can go on and opening the web server, opening the port 80, and the web server socket will stay open to accept the incoming connection. So you will have a usual thread, for example, but you will have also two timers. This morning, Manuel discussed, showed you some component, some extra component inside the Azure AirTos library. Those are, for example, the software timers. This task, this library needs two more timer other than the thread itself. One slow timer and one fast timer. Why the fast timer? Okay, the fast timer is the engine, let's say, of the library, so not a big deal. The slow timer is usually fundamental for, for example, for the reliable connection, for the TCP-IP connection. If you have a reliable connection, your system, your library must be aware that something wrong can happen or must be aware if something wrong happened. This way, he needs to be advised by the timer that is taking care about the timeout, think about the TCP. And if something bad happened, say to the application that something bad happened and the application or the library itself is able to, for example, close the TCP socket, the reliable socket. Even if the acknowledge are lost or even if on the other side something bad happened.


This is stock number 9J378B. We are here at Summit Automotive in Fond du Lac, Wisconsin. You're new and used car headquarters. Today we are checking out this super clean 2011 Buick Lucerne. This vehicle has the 3.9 liter V6 motor which pumps out 227 horsepower. It has been fully safe and inspected by our service shop has a fresh oil and filter change. All the fluids have been checked and topped off and this car is 100% ready to go. Red crystal tint coat is the color. We shoot all of our videos in 1080p, 60 frames per second. So if you have HD capabilities on your computer tablet or smartphone device, turn them on right now because it's like you're right here looking at the car with me. And definitely your best way to check out the vehicle quality and condition before seeing it in person. If you want to check out all the photos of this car in the upper right hand part of the screen, link right to our website, click the end check us out. Looks like it has brand new brakes on the front and it has Edvanta HPZ tires on here. 235, 55 R17 is the size and these tires look like they are brand new as well. So brand new brakes and tires on the front. Driver side fenders in excellent shape. Front bumpers in excellent shape as well. No dense things or cracks on that. The hood is in really nice condition. I didn't see any major dents or dings on it. Passengers side fender is in great shape too. And the passenger side rim is very, very clean. As you go down this side of the car, you can see just how clean the body is, how reflective and mirror-like that paint is. We take these HD videos so if you are far away or even if you're close by and just cannot make the trip down but you're still interested in purchasing the car, you can see the car, hear the car, and have confidence in the vehicle that you're looking at before you even get here so that you can make a smart buying decision from the comfort of your own home. And that's why we offer the Summit Auto Fastpass option in the upper right hand part of your screen. Another link to our website where you can apply for financing, get pre-approved, see a payment quote, even appraise a trade-off from the comfort of your own home. Looks like the back brakes are brand new as well and the back tires have about half the tread on them. As we come around to the back of the vehicle, the rear bumper is in pretty nice condition. It does have the chrome-tipped exhaust. The trunk lid is in nice shape as well and we'll take a look inside there in just a second. It has the backup parking sensors but you can see the bumper is in real nice condition. And as you go down the driver's side just as clean as that passenger's side and for full disclosure this back rim is in nice shape as well. I didn't see any dents or dings on the doors. Inside the CXL package gives you the tan leather interior. There are no rips or tears on these seats. You do get a power driver seat and a power passenger seat. This one has auto headlamps, your information center, power windows, power locks, power mirrors. These front seats are heated and you do get a memory driver seat. We'll hop inside here and then we'll have to take a look at the trunk in just a second. You can see that this car only has 61,906 miles. There we go, I got the trunk open. Instrument cluster is very nice and clean. You get the heated leather wrap steering wheel. That's in excellent shape. Bluetooth and cruise controls or I'm sorry Bluetooth and information center controls on the right. Cruise controls and heated steering wheel buttons on your left. It does come with the CD player and FMN series 6M radio capabilities.  It has the nice wood grain trim, the leather wrap dash, passenger seat is in excellent shape as well. No rips or tears on that. It does have the 40, 20, 40 split bench seating and the headliner is in really nice shape as well. Home link buttons, map lights and on-star capabilities in that mirror. We'll take a look at the trunk back seats and then we'll check out under the hood. Lots of room in the trunk back here and I believe it's got underneath there is going to be your spare tire. Lots of room back here. We'll take a quick look at the back seats and then check out under the hood. Back seats are just as clean as the front seats. No rips or tears back here.  Factory floor mats, carpeting is nice and clean and then you do get the child safety locks on the back doors. We'll take a quick look under the hood. I would personally like to thank you for checking out the video today and hopefully from this HD video we'll be able to tell just how clean this car is all the way around. Inside out under the hood we have the 3.9 liter v6 motor, 227 horsepower engine bay is very clean, runs very smooth. This car has been fully safety and inspected by our service shop, has a fresh oil and filter change. All the fluids have been checked and topped off. The car has been gone through mechanically 100 percent, has brand new brakes all the way around, brand new front tires and is 100 percent ready to go. To see more pictures of this car or one of our other 450 new and used cars, trucks, SUVs, minivans, Wranglers, you name it we got it go to our website www.summitauto.com. Full pictures and descriptions of every single vehicle from two locations all at summitauto.com. If you'd like to check out more HD videos you can go to youtube.com slash summit auto remember to like, subscribe and share on this video and all the videos that you see there. In fact in a second you will see links to subscribe to our youtube channel on your left. A link to more used inventories like this one on your right. If you've not been to our website on the bottom link to this vehicle on our website click those check us out and we really look forward to helping you with this super clean 2011 Buick Lucerne CXL. Thanks again.


Hello everyone and good morning thank you Ravi for this kind introduction and today I would like to talk a little bit about this unique protein which is called the musin and I will show you how we are trying to develop a specific and selective fluorescent probe for musin. So for the next 15 minutes I'm going to start with the basics. So first of all what is musin? Well musin it's one of the biggest and more complex protein within our body. Think that more than 5,000 amino acids compose a single monomer of musin and to most of these amino acids are bonded sugars which are called glycans and these glycans can be up to 80 percent of the total mass of the protein and because of the presence of the glycans musin can retain a huge amount of water and because of its physicochemical properties musin can assemble into this polymeric glycol conjugates which forms a three-dimensional network around which mucus is organized and you have to know that basically we have mucus all over the wet surfaces of our body starting from the eyes going to the airways and up to the gastrointestinal tract and these three-dimensional metrics formed by mucus it's extremely important for us because it is the first barrier of the organism against the penetration of you see viruses bacteria but also drugs and unfortunately we know really well musin because of the so-called mucus-related disorders like cystic fibrosis and COPD where musin it's overproduced and it generates a viscous mucus but in addition to these mucus-related disorders in the last years we are facing an increased interest on musin and we are gaining more and more scientific evidence about the implications of musin in different kind of tumors and for example it has been proposed that secreted musins could be a diagnostic and prognostic biomarker in pancreatic cancer which you can see it's the third most deadly type of cancer worldwide and today one of the most interesting I would say methods to detect biomarkers at serum level consist indeed of fluorometric detection mediated by fluorescent probes and these techniques are really interesting because they are simple and relatively cheap and most importantly because they are extremely sensitive and we got inspired by this work where the the authors developed a fluorescence probe to detect lipase at serum level and with this probe they were able to discriminate between healthy people and people having increased serum level of lipase and so the idea our idea it's kind of similar because we are trying to develop a fluorescent probe to be used to detect musin at serum level and potentially to use this fluorescent probe to to screen between healthy people and people at risk of pancreatic cancer and today among the most interesting fluorescent probes we have definitely the the polymethane dyes such as quareins, croconins and cyanines. Structurally these dyes are formed by these poly conjugated bonds flunked by two two moieties, an electron donor and an electron acceptor and sorry and they are really interesting because they are quite photo stable in in solutions and they have really high absorption and emission properties and their photo physical properties can be easily tuned by changing the length of this of these poly conjugated bonds or changing the the functional groups and for our purpose we focused on squareins because in the last year squareins have been widely studied and they found many applications spanning from solar cells to light emitting diode and even they have been proposed for biological applications. So as I was saying previously squareins as well the other polymethane dyes are characterized by really high absorption coefficients and the high quantum yields and high fluorescence intensities but this happens only when they are dissolved in organic solvents in fact the main drawback of squareins as well the other polymethane dyes is that as soon as they get in contact with water they form insoluble aggregates and the fluorescence it's completely quenched. But an interesting phenomenon happening with squareins is that when they get in contact with specific proteins they can increase their fluorescence and we call this phenomenon a turn-on of fluorescence and the idea behind this project it's trying to exploit this phenomenon to develop a fluorescent probe and detect musing using indeed the turn-on of fluorescence. So we started by investigating these four squareins you see all of them have a negative charge at pH 7.4 because of the carboxylic group on the lateral moieties and they belong to two different classes one being the indolinine-based and the second the benzindolinine and the other differences between them consists of the length of the lateral chain you see here we have two carbon atoms while he ate carbon atoms. So we started by studying how these molecules interact with the musing by fluorescence spectroscopy and here we got really good results because for all of the four squareins we got an increase of the fluorescence. You see with the squareins with the short alkyl chain we got a mild increase of fluorescence up to fourfold while the squaring with the longer alkyl chain we got a higher increase of fluorescence up to 45-fold and yeah this was quite a good result but the initial joy was quickly dampened because then we repeated the experiment with using instead of musing we used albumin and this time we got even higher increases of fluorescence and for our purpose such a result it's not good at all because as you can imagine at serum level the most abundant protein is albumin and consequently when detected musing in serum it could induce a really strong background signal so again our purpose is to develop a fluorescent probe which responds to musing but not to albumin. So we next try to understand if what is the mechanism behind this phenomenon so we had a look at the structural activity relationship and here we found an interesting behavior for example if you have we have a look at the turnon we see that the squareins with the short alkyl chain can induce a quite mild increase of fluorescence while the other with the longer alkyl chain have really strong increases of fluorescence and we also investigated the kinetics of the interaction by monitoring the time requested to reach an equilibrium and a sort of a plateau and here again we see that the more complex is the squaring and the longer is the time requested to to reach the stability we also calculated the dissociation constant which gives us information about the affinity between the the squareins and and musing and again we see a kind of relationship with the structure of the squaring because the the more complex is the squaring the lower is the affinity for musing and we then put together this experimental data and computed a correlation matrix with some molecular descriptors and here we got quite an interesting result because we found that lipophilicity could could modulate the interaction with musing because for example these molecular descriptors the molecular weight the total surface area the partition and the distribution coefficient all of them are related to the lipophilicity of the of the molecule and basically all of them are strictly correlated and they give us the same information which in other words is that the higher is the lipophilicity of this of the squaring the higher will be the turn on a fluorescence but at the same time the higher the lipophilicity the lower will be the affinity for musing and the longer will be the interaction time and this actually could make sense because when we study the interaction by absorption spectroscopy we see that increasing the concentration of the of musing actually increases the band corresponding to the monomeric form of of of the squaring while at the same time the increase of the protein concentration decreases the band corresponding to the H- aggregates of the squaring so we speculate that the mechanism behind the interaction with the protein could be a kind of desegregation and solubilization on the lipophilic spots of musing because you have to know that in addition to these really hydrophilic domains which are heavily glycosylated musing has also naked portions of the peptide core which are called cysteine rich domains and usually this falls into more hydrophobic domains and so we speculate that the aggregate of squaring could attach and interact on on these hydrophobic spots so according to to these observations then we wondered if we could increase the selectivity for musing by increasing the lipophilicity of the squaring so what we've done was to synthesize new squarings and the first thing we've done was to get rid of the carboxylic group on the lateral moieties because they are quite hydrophilic and then we increase the length of of the lateral chain up to four carbon atoms and we obtain this new squaring which we called it C4 squaring C4 then we recorded the interaction again with the two proteins and this time we see that the signal we record with musing and with albumin it's quite overlapped so this means that on one hand we reduce the selectivity for albumin and on the other hand we increase the the selectivity for musing so it looks like we really modulated the selectivity for musing by modifying the lipophilicity of the squaring so in the next step we wanted to increase even more the lipophilicity of this structure so we introduced two bromide atoms on on the lateral on the indolinine groups and we obtained this bromide C4 squaring and for the first time we were able to get higher responses to musing with respect to albumin and this is really a great result but still we we have a response to albumin and for our purpose it's it's not suitable because again we are we want to have a dye which responds to musing but it's not sensitive to albumin so because of this we wanted to increase even more the the lipophilicity of the bromide C4 and this time we introduced the benzoindolinine moiety instead of the indolinine and unfortunately this new structure this new squaring was so lipophilic that it was completely insoluble in water and it's actually is quite insoluble even in organic solvent and the signal recorded was not reliable so in this case we we had a solubility solubility problem so what to conclude what i've just shown you is just is that squarines could potentially be used as fluorescent probes to detect musing because they can increase their fluorescence in the presence of of musing and among the structures we studied we found that the bromide C4 is the best their former because it was more selective for musing with respect to albumin but we are we plan to to modulate the structure of bromide C4 to increase its response to musing and we also plan to test positively charged squarines because we speculate that cationic squarines could interact with the negatively charged glycans present on musing by electrostatic interaction but we still have to do all of this and let's see and finger crossed so i would like to thank my supervisor professor Sina Vicentine and Nadia Barbero and Carlotta Pontremoli from the Moff lab at the department of chemistry here at University of Turin and last but not least for sure i would like to thank you for your attention and i'll be glad to take your questions thank you thank you cosmon i appreciate you finishing well in time so we have time for questions full five minutes okay let me see if there are any hands hi rally i actually have a question yeah sure so yeah luka and then um hankona after that okay look yeah yeah first very nice presentation thank you um i just had a couple of so i saw that actually you do see a shift in the emission when you when you move it to the in some yeah some cases it's more it's more uh it's stronger like uh when you have this 45 time announcement you also have a shift uh in the in the emission peak so first question is could you use this as a as a way uh beyond the intensity and the other question if is if this protein has a structure that was resolved ever so that you could maybe uh identify better which are the side the position where the dive bind to the protein yes thank you okay thank you luka really interesting questions i will start with the first one uh yeah this could be uh one of the solution but i don't think uh it would be reliable because we observe this kind of shift not only with musin but also sorry also with albumin and even with other kind of proteins because we are testing now for example we tested the trypsin the fibronogen and the different other kind of proteins because uh of course at serum level we the most abundant protein is albumin but it's not the only protein so again we we will have the same problem because the shift will be observed even with the other problem with the other proteins and regarding the structure of musin uh today we don't have the complete structure uh we have some portions for example we have uh resolved the some hydrophobic domain some for example the foam filler brand which usually is at the bottom of the protein but we don't have the complete structure especially because musin as i as i was saying it's highly glycosylated and um we don't know exactly uh how the sugar chains are attached to musin so yeah i hope i i answered your questions okay now ancona you had your question yeah i think my question already got partially answered because i was going to ask whether you have tested with other proteins that would be present in serum and it seems like this probe is also interacting with other proteins yeah uh actually yeah we um actually published that that work and we saw that for example with fibronogen and trypsin we had really high uh signals and uh but usually the most uh reactive protein i would say remain albumin so yeah thank you thank you yeah i don't see any other hands so kosmin i get a chance to ask a question so um is musin a membrane bound protein actually does it have a transmembrane part yeah there are two kinds of musins the secreted and the transmembrane musins the for example the transmembrane musin are attached to the cellular membrane with the an intracellular domain and usually this kind of musin constitutes the first layer of mucus which is actually it's more hydrated but at the same time there are different other kind of musins which are the secreted one which constitutes the upper layer of the mucus with the the reality dimensional network created by by musins yeah is it so then and i assume that you're trying to detect the soluble musins right the one that actually gets secreted out isn't that correct uh well we are using commercial musins in this case we used porcine gastric musin which is extracted from the stomachs of pigs and um yeah the the most part of it is definitely secreted musins but uh we cannot be sure that uh among the the secreted musins are are present even transmembrane musin because of you know of the extraction process and maybe part of the transmembrane musin is also present but it's mostly it's mostly secreted musin right so yeah so then that's so that's what my confusion is actually so how does increasing i thought i saw in your data that the lipophilicity anti correlated with affinity right initially right which makes sense based on what we just discussed but then later on you increase the lipophilicity and you got stronger binding i didn't make sense of that if you why why does that happen which is this this slide you're referring maybe this one yeah this one this one tells me that lipophilicity is oppositely correlated to affinity for example right yeah right and now if you go to when you went to your square ends you try to increase the lipophilicity of the square ends and you got a better yeah because yeah because even even though the the response to musin it's higher with the the lipophilicity of the square end the this is not related to the affinity we saw and also because we are not sure if we could talk about affinity in this case because we are looking at the interaction between an aggregate and a protein and not an interaction between the single molecule and the protein so i'm not sure if we can talk about affinity oh okay okay so so you're just increasing the ability to form the clusters the aggregates so that you can actually interact with them okay exactly yeah yeah okay makes sense okay thank you cost man i think that answers my question for all the all the questions yeah sure okay great so i think that brings us to the end of the day i think i will hand it over to luka at this point to make any final announcements


Look at a May 40 here hanging out at Kudji Beach Monday afternoon about 5 p.m. January 16th and thinking about family, friends and careers. And before my father got married he told his two wives that his career would always come first meaning his Christian missionary, he wasn't just a career, it was a mission. So I like that analogy, think of a hot stove with four burners. You can't really run off four burners at four bore. So one burner represents family, one burner represents career, one burner represents friends, one burner represents health. Most of us can only keep really two burners going for bore. Usually we choose family and work. So my father put his career first and it's a little bit like that song, we'll get together soon son, right? Cat and the Cradle, Cat Stevens, we'll be together soon. The boy who remembers his father never has time for him and then when the boy grows up and the father now wants to spend time with him, the boy tells him, oh dad, we'll get together soon. Sure dad, we'll get together soon. So because my father put his career first, I kind of thought that was normal and so one of my happiest memories growing up were outside of home and I kind of thought it would be awesome to become an adult because then I could just hang out with my friends 24-7 without any interference from my parents. And when I get older, I'll just be friends because then it'll be friendship based on common things that we have in common, not just accidents. So I was kind of looking forward to just growing up and just spending time with friends rather than family. And then the older I get, the more important I realize family is. But I think I unconsciously took on my father's career first, mission first attitude. And so I'm kind of a bash. Look, what about the Revolver News interview with Donald, hearing it now on Rumble 2X? Make sure to skip past the election chatter. Okay, when does it get good, the Rumble interview with Donald Trump? At what point in the interview does it get good? So I think for some people, like my father, putting mission first, I mean putting first that thing that gets you the most love in life, right? We all tend to prefer to do things that we're really good at and not spend so much time with things we're not good at. And so some people just not so good with intimacy, connection, you know, horizontal relationships. And so when my father had so many fans available to him, perhaps, you know, the messier world of family was discouraging. So he lost the election by 31,000 votes in 2020. Yeah, I heard if somewhere between 30 and 50,000 votes got the other way, he would have won. And if he'd only appeared to have handled COVID confidently, let's say he did or didn't handle it confidently, if he'd just appeared to have handled it confidently, if he didn't have those rambling news conferences every day about COVID, he would have won. He was headed for re-election, but he really messed up with regard to how he came across in dealing with the COVID crisis. That's what cost him the election. So yeah, I'm sitting here at the park, you know, looking at a lot of happy families and just kind of thinking, I unconsciously took on from my father this idea of, oh, just be with friends. One burner represents YouTube, one burner represents Jewish conversion, one burner represents air supply. One burner represents the Dallas Cowboys who play tomorrow. So I think for some great man like my father who was regarded as a great man by thousands and thousands of people, right, how could family compete with the adulation that he got from having followers? You know, strongly committed fans. I think some people who produce content on YouTube or the great men in public life, they're so used to adulation that horizontal, familiar relationships or relationships with people who knew them before they were great, just not nearly as satisfying. And I think that's kind of what I wanted to build when I was a kid.  Then I won't have to mess around with those messy, awkward relationships.  I'm just going to make myself into a great man who receives excessive amounts of adulation. And now I'm older and wiser, right. I've walked about 10 miles on shabbas and as I was walking along much of it, it's like, okay, let me meditate on the principle with regard to step one acceptance and so let me accept my awkward self. Trump speaks for 46 minutes. Not sure it's exciting. Trump seems like an Alex Jones show investor. Yeah, he does because I think Alex Donald Trump, you know, turns to wherever he gets the most attention, the most love. I think, you know, we all prefer to do that, which we're good at. We all tend to grow towards attention and love. And if Donald Trump is getting more of it on the Alex Jones show or the Nick Fuentes show, then I think that's where he's going to turn. Particularly if you have no core sense of self, you're going to turn. Look, the Crow wants you to leave their turf. They want you to leave or feed them. Hey guys, I'm trying to run a high quality live stream here. But I'm reading this book, The Power Broker on Robert Moses, right. It's a book by Robert Caro. Here we go, The Power Broker, Robert Moses at the Fall of New York. So between about 1930 and 1960, Robert Moses was perhaps the most powerful man in New York City. He was a fox commissioner, a builder, a planner, a powerful figure behind the scenes. And he completely fell out with his brother. And his sister, who adored him, he completely fell out with her too. And when she wanted to get together with him, he told her, oh, I'll be at the airport. You know, you can drive several hours to see me if you like. And I can spare a few minutes for you. So his brother was like starving. His brother was in dire straits. But Robert Moses put the word out that nobody should hire his brother as the engineer. Because I think that awkward familiarity with family, people knowing you when you weren't a great man, must have worn you down. Impressive stream output, wonder what your show would be like if you stayed in Australia. Not enough tent people, mate. So, yeah, reading about Robert Moses, right? The power broker, right? So he's so loved being a power broker. He's so loved being able to make and break people. He's so loved being the most powerful man in New York City. He didn't really want to have anything to do with people who knew him when he wasn't so powerful. Or when people could have a, you know, familial pull on him. And it just reminds me kind of my, you know, fantasies when I was youth.   And I wouldn't have to bother with the messy, awkward world of human relationships that I could just have followers and I could just get my adulation from them. Oh man, beware of the anti-Semitic crows. I just had the worst experience with my iPhone. Okay, so I was trying to activate text-to-speech. So I went to my iPhone and I went to accessibility and then never go on your iPhone and turn on voiceover at the top. My iPhone just went out of control and just started issuing all these commands like all the familiar ways that I'd once controlled my iPhone. You know, like Robert Moses wanted to control everything and everyone around him. I thought I had control of my iPhone, but I made the huge mistake of putting my voice over on, completely lost control of my iPhone. It just starts, you know, issuing all these orders to me. And then as I'm trying to get out of this thing, I accidentally triggered the emergency activation where if you hit the right side button, I think five times, I was trying to break out. I think I hit this right side button five times. Okay, countdown to send emergency notification. It took me about 15 minutes to get out of voiceover. So whatever you do, never activate this voiceover function under accessibility on your phone. I just wanted to activate text-to-speech, but that was an absolute nightmare. Oh, ethnicity isn't always so good. So Australia soccer leagues used to be organized on ethnic lines.  So in Ontario has a public park with porta-parties, a few dozen large tents, L.A. Portland style of supporting permacams. Seems like you activated self-destruct. Yeah, I essentially activated self-destruct on my iPhone. It was a nightmare.  I'll never get that time back. Oh, anyway, so Australia's soccer leagues used to be organized primarily around ethnic lines. So it was even like the Jewish club, Greek, Italian, all these different Anglo clubs, and they would get into fights, particularly when Yugoslavia had civil war. The Australian soccer clubs, right? The best Australian athletes tend to be Croatian. The Australian soccer team used to be Australian Croatians. So when Yugoslavia descended into civil war, we'd have these pitch battles on Australia's soccer turf. So it turned out to be much better when they shifted Australia's soccer league away from having ethnic ties. So I tend to think, you know, strong in-group identity, good thing, strong ethnic identity, good thing, like organizing around what you have in common, such as ethnic identity or religious identity, that's a great thing. But it really wreaked havoc with Australia's soccer system. So now they've just got, you know, I think two clubs in Melbourne, two clubs in Sydney, completely cut ties with the ethnic element. So I know that there's, like, a soccer club in England which is, you know, reputed to be Jewish and is regarded, you know, spoken about as Jewish. But Australia's soccer league, organized by the Loewe family, Frank Loewe, I think he organized a $20 million push to bring the World Cup to Australia in 2022, but instead, Kuwait beat them out. I guess Australia didn't provide the necessary bribes. We were too Anglo-Saxon about it. But anyway, Frank Loewe was the shopping centre magnate. Hollywood's teeth are brighter than the white paint. It's the apricot sky, too, filming when he returns. So anyway, this orthodox Jewish guy, a Holocaust survivor, was a major force for removing the ethnic element of Australia's soccer clubs, turning them into more professional clubs like you have elsewhere in the world. But when Australia has ethnic soccer clubs, there's just, you know, war on the pitch. So sometimes increasing your in-group identity is not such a great thing. Usually it's a good thing.  But sometimes in certain areas, right, like perhaps in the workspace and sometimes in sports and sometimes in other activities, it really helps to de-emphasise your in-group identity, certainly help Australia's soccer league have to completely remove the ethnic element, make it more professional, and thank God there was a Loewe family in Frank Loewe who was able to assist them and fund them towards removing the ethnic element. Bye-bye.