How's it going everyone at Caleb here with late and sports cards next break tonight is gonna be ww revolution 2022 one box break in number 15. Thank you again once all for joining I got the results for you right here. Thank you once again all sorry messed that up a little bit Eric Josh and then Coon and then Derek with the rest. Let's do it 15 here Good luck. No autos yet tonight no crazy galactic or anything like that. So maybe we can get lucky Here we go Eric Cowboys fan feel like that happens man people always are Taking their cowboys are taking the Packers regardless of where they live Steelers Steelers. Yeah, all right Alexa Bliss riddle We got big Nice look at these as well. This is gonna be an angular to 199 Eddie Guerrero.  Okay, Josh B second pack. Good luck Josh. Thanks for getting in man I don't know how I feel about Mike being a Cowboys fan Little put off by that. Oh, no, Rick. Well Gonzalez for you. We got Carmela Marcelle Barthel It's gonna be answering the giant very cool fractal not numbered. Nice, man. There you go Josh Very cool and million-dollar man Ted Diabosi Okay, here we go. Good luck Coon Coon s third pack. Let's get to it. All right. Here we go. Shelly Gigi Dolan live Morgan We've got Astro AJ Styles and ooh supernova Charlotte flair cubic very nice Cubics are tough hits. That's gonna be numbered 48 of 49 Nice Charlotte flair supernova cubic inserts awesome one there congrats Coon s Okay, the rest of the box here is gonna be for Derek a one two three four five packs Good luck man. Derek a the rest is yours here Let's get right to it right to it. All right Cameron Grimes Trying to figure out the best way to hold these with the lighting. There it is Candice LeRae Nash Carter, it's gonna be a Kofi Kingston King Woods the new day insert there Astral parallel and a Randy Orton Here we go. Grace and Waller Shayna Baszler Kofi Kingston It's gonna be a Sunburst 1099. I believe you up to 99 Odyssey Jones rookie card.  It's gonna be a groove there Bianca Belair not numbered group and dusty roads Look Derek Chad Gabel Santos Escobar there we go Drew McIntyre Otto number 299 Sunburst parallel Congrats Derek. Very nice Figured you're kind of due for an auto here. Yeah after the first two didn't have one Nice one Derek congrats. Maybe Derek was thinking the same Shinsuki Nakamura Astro and Vortex Kevin Owens One more pack. Good luck. Thank you again Derek Mansour Ronda Rousey heaven Owens. It's gonna be a Fractal. Yep, Fractal Zashili and Triple H. Hey, there you go. Thank you again everyone that got in that one WWE Revolution random pack style break number 15. We'll get that uploaded to the site. We'll move right along to number 16


I don't know where to begin with the good news or the bad news. So maybe I'll begin with the good. There is less terrorism today in Pakistan than there was last year. And there was less terrorism last year than there was the year before that. In fact, if you look at the fatalities, there was something like 7,500 in the year 2011. I'm sorry, there was 6,000 in the year 2011. And there were 7,500 in the preceding year. So there's been something like a 20% drop in the number of fatalities due to terrorism in Pakistan. The difference is also palpable. You can feel it in the cities. The barricades are fewer, they are less heavily armed, and you can get by from point to point in less time these days. It used to take me something like 30 minutes to get from my university to the center of the city last year. And now it takes me 20 minutes because the barricades are fewer. If one looks at the number of suicide bombings in Islamabad, we haven't had one since I think nearly two years now. In Lahore, there had been a number of suicide bombings, not one also I think in the last two years or so. Peshawar is not that good. There has been one in the last month or so. Of course, if one goes further away, if one looks towards Fata, the frontier province, well, there are more suicide bombings. And the fact that 6,000 people were killed in suicide attacks, bombings, and were killed by the army in the action against militants, all that is saying that things are better, but in no way can they be called good. Well, I've told you the good news. Now let me tell you the bad news. There's been a quid pro quo for this, and that has been the acceptance of the militants as being a political force. And one sees that in the last several months, there have been several major rallies in which hundreds of thousands of people have participated known as defense of Pakistan rallies. So they were held in Lahore, in Karachi, in Peshawar, in many other places in the country. At the podium were militant leaders whose names you will recognize, people like Hafiz Said of the Lashkar-e-Tayabah, renamed as Jamaat-ud-Dawah. You had on that same podium the Tehrik-e-Nsaaf, Imran Khan's party, although he did not personally appear over there. You had the famous Shia killer, Malik Ishaq, who was freed from jail after he terrorized the judges. When he was taken to court, and the charges were read out against him, he looked at one of the judges and says, I know who your children are. The judge tries to hide his face in his hands, but when he reads out the names of his children, he gives up. So Malik Ishaq today is a free man. As I go from my university towards the city, on the way is the Lal Masjid, which you have heard of as being the center of insurrection. In 2007, when they took over the children's library and they took over other government buildings and they kidnapped women who they said were prostitutes. And then they went on a rampage across the city, they burned video stores, they had massive bonfires and so forth, after which the military finally had to act. It acted after some Chinese were kidnapped from a Chinese brothel. And then, as you know, we had a major war within Islamabad. From where I was, you could see that rockets were being fired. You could see that heavy machine guns had been used. The other side, I mean not the government side, the Mullah side says that hundreds were massacred by the army. The army says only 130 or so were killed, but this is now happening within the heart of Pakistan's capital. So you might think that something big would have happened in consequence. Well, the big thing is that there was a war, there were people who were killed, but should you go buy the red moss today, you will find that it has not only been reconstructed, but it has become many times better looking than it was years ago. And moreover, you will see over there, and this I saw last week, there was a big board over there that's been there now for a few years, which says that, it says in Urdu that here is a place where peaceful Muslims were praying and the horrible animals of the government, they attacked them and destroyed peaceful Muslims, etc. Now the interesting thing is that this is happening on government land. That red mosque is government property. The Imam of that mosque is paid by the government of Pakistan. This is a mosque which was in open rebellion against the state, and yet today it exhibits a board over there saying that we fought off the state, the Pakistani state. And so the response of the state has been to not only improve that mosque but to give it land elsewhere for constructing Jamia Hafsa. What I'm trying to say is that the state has covered its shrunk before the assault of the militants. In places it has tried to accommodate them, given them a place where they can now freely speak from the podium. In other places where the militants are less amenable, they are being fought. Now what has been the cost of accommodation, of bowing before these forces that the state had earlier on been fighting against? The principal cost has come to the minorities of Pakistan who today feel especially embattled. One sees this for the Ahmadis, for the Christians, for the Hindus, but they were minorities to begin with. Now there is another minority that has been created in Pakistan. It's a big minority between 20 to 30 percent of the population and it is the Shias. Over the last several months there have been massacres of Shias carried out by the Lashkar-e-Jhangvi, which is a banned organization but which now functions in full view of the Pakistani public. Therefore what we are seeing is the state retreating and giving more and more space for militancy to take over and for radicalism to spread. The cost of this is going to be felt not just by the Shias but eventually it will be felt by everybody because today there is a war that is not yet out in the open but which could explode and that's the war between Diobandis and Barelvis, the two major Sunni sects. Why is this happening? Well in part it's the steady radicalization of Pakistani culture, a process that started at the time of General Ziaul Haq, a process that was made permanent essentially by having school books that contain hate materials. It is also a process that at that time suited the Pakistan army very well because it was from that society radical elements could be freely drawn to pursue strategic objectives first in Kashmir and secondly to achieve strategic depth in Afghanistan. Now that process although it is still intact and has not been dismantled is under some pressure. That is because those creations, those Frankensteins have turned upon their creators and so over the last several years we have had important army institutions that have been attacked and this includes the general headquarters of the Pakistan army. Three major centers of the ISI have been blown up and turned into rubble. I saw the one in Peshawar, it was actually just bricks that had been left over after a suicide bombing. We have seen the massacre of army officers as well as that of their children at the parade ground mosque and now there is a deep fear within the Pakistan army that this monster has gotten out of control. But this fear has existed for a while. Why is it that in the last year or so we have seen lessening of that fear and in fact as I said permission being granted to those people who had been earlier thought of as national security threats to now engage with the public. A good part of this reason comes again from strategic imperatives. As the Americans prepared to withdraw from Afghanistan in 2014, Pakistan now sees a new opportunity that is opening up over there and is not willing to forego that. That is one reason. I am not sure it is the most important reason but it is an important reason. A second reason is the deterioration in ties between the United States and Pakistan. As we well know and in fact public polls have shown this, the most unpopular country in Pakistan is not India as it once used to be. It is the United States. Particularly after Osama bin Laden and then after the Salala incident in which 26 Pakistani soldiers were killed by NATO forces, relations have plummeted and you could say that there is almost a hysteria against the United States in Pakistan. Now responding to that, the army leadership, General Kehanyi and earlier General Pasha has been replaced, have now realized the temperature that lies in the ranks below and not very far below even in the officer's core and so therefore they know that they cannot now secretly support drone strikes as they had earlier. Obviously they are worried, after all we see from WikiLeaks and looks pretty genuine that General Pasha was worried about the Taliban taking over Kabul. We don't know but it is quite credible that that should be the case. What is perfectly credible is that those WikiLeaks reports which were leaked showed that the army high command had asked the Americans for more drone strikes because they after all do see the Taliban Pakistan and other elements of the Taliban as being genuinely dangerous for them. Let me speak to you about the danger to the army. It is absolutely something that possesses them. Today our army officers do not dare to wear their uniforms in public. Once upon a time it used to be that they could walk into a store, into a shop, into a business and people would get up to salute them. They would come in in their uniforms. In fact if you had a problem and you knew an army officer you would say can you come with me? I have a problem over there and your problem would be resolved. Today they cannot get out of their bases, out of their homes wearing their uniforms. No longer do they go in official army cars. They have private cars with these guys' numbers. Now that has been the cost and so the army genuinely has been at war with a fraction of the militants. And yet in its state of extreme confusion it cannot take firm action where action needs to be taken. Running with the hairs, hunting with the hounds, that was the earlier philosophy. It's still there in part. The Americans have made it more difficult after Osama bin Laden to pursue that policy of strategic duplicity but there is no clarity. There is fear in the upper army leadership and there is a strong anti-American pro-Islamic sentiment in the ranks below. I'll end over here and say that things don't look good. Once again we see that strategic imperatives are taking over as the priority instead of domestic peace but this has frightful consequences for the future. If men like Malik Ishaq who are advertised, self-acclaimed Shia killers, if men like Hafiz Saeed, many others who I could name are allowed to go before the public and be presented as genuine political forces, well then one needs to fear. Thank you.


qualifying child of more than one person. So even if a child meets the conditions to be the qualifying child of more than one person, only one person can claim the child as a qualifying child for all of the following tax benefits unless the special rule for children of divorce or separate parents described earlier applies. So the general rule is again, you can't have multiple people getting all these benefits for the same person with the same social security number that will cause problems. Number one, child tax credit and credit for other dependents that's line 19 and additional child tax credit line 28. Two, head of household filing status. Three, credit for child and dependent care expenses. Four, exclusion for dependent care benefits. And five, the earned income credit. No other person can take any of the five tax benefits just listed based on the qualifying child. If you and any other person can claim the child as a qualifying child, the following rules apply. For purposes of these rules, the term parent means biological or adopted parent of an individual. It doesn't include a step parent or foster parent unless that person has adopted the individual. So if only one of the person's is the child's parent, the child is treated as a qualifying child of the parent. If the parent file a joint return together and can claim the child as a qualifying child, the child is treated as the qualifying child of the parents. If the parents don't file a joint return together but both parents claim the child as a qualifying child, the IRS will treat the child as the qualifying child of the parent with whom the child lived for the longer period of time in 2022. So if nothing else, that's usually gonna be the distinguishing factor where the child lived for more of the time frame. So if you end up in a weird fight like that, if it comes down to what it comes down to, that's usually the breaker. So if the child lived with each parent for the same amount of time, the IRS will treat the child as the qualifying child of the parent who had the higher adjusted gross income, the AGI for 2022. If no parent can claim the child as a qualifying child, the child as treated as the qualifying child of the person who had the highest AGI for 2022. If a parent can claim the child as a qualifying child but no parent does claim the child, the child is treated as the qualifying child of the person who had the highest AGI for 2022, but only if that person's AGI is higher than the highest AGI of any parent of the child who can claim the child. Example, your child, Jay, meets the conditions to be a qualifying child for both you and your parent. Jay doesn't meet the conditions to be a qualifying child of any other person, including Jay's other parent. So then under the rules, Rules, rules, rules. Just described, you can claim Jay as a qualifying child for all of the five tax benefits just listed for which you otherwise qualify. Your parent can't claim any of those five tax benefits based on Jay. However, if your parent's AGI is higher than yours and you do not claim Jay as a qualifying child, Jay is qualifying child of your parent. And you can see how that situation could be beneficial because you might want that to be the case because if you claim it, the parent claims it and they have more income from a total income tax perspective with everybody involved, it might be a more beneficial situation. So for more details and examples, you can see publication 501. So if you've got some, there could be some strange family arrangements and whatnot, they can twist the tax code into kind of knots. So if you have some of those, you can see more examples on that publication. So if you will be claiming the child as a qualifying child, go to step two, otherwise stop, you can't claim any benefits based on this child. So we got the social security numbers. So you must enter each dependent social security number, that's the SSN, be sure the name and social security number entered, agree with the dependent social security card. Otherwise, at the time we process your return, we may reduce or disallow any tax benefits, such as the child tax credit, which is a big one, of course, based on that dependent. If the name or social security number on the dependent social security card isn't correct, or you need to get an SSN, social security number for your dependent, contact the social security administration. You could see social security number earlier for more information there.  The required social security number is one that is valid for employment and that is issued by the SSA Social Security Administration. So I'm not gonna go into that more detail here because we've seen a little bit of that in the past. If your dependent child was born or died in 2022 and you do not have a social security number for the child, enter died in column two of the dependent section and include a copy of the child's birth certificate. If you or your spouse is filing jointly, didn't have a social security number or I-10 issue on or before the due date of your 2022 return, including extensions, you can't claim the child tax credit or the credit for other dependents on your original or amended 2022 return. If you apply for an I-10 on or before the due date of your 2022 return, including extensions and the IRS issued you an I-10 as a result of the application, the IRS will consider your I-10 as issued on or before the due date of your return.


Hey guys welcome back to Come Again TV the only place on YouTube we're all geek culture collides. I'm Shannon and today we're going to be taking a look at the Ghostbusters Answer the Call 2016 Gillian Holtzman action figures so stay tuned. Hey guys welcome back to Come Again TV the only place on YouTube we're all geek culture collides if you're new to the channel don't forget to hit that subscribe button so you don't miss out on future videos. As I said today we're taking a look at the Ghostbusters Answer the Call 2016 Gillian Holtzman action figure. This is a figure I've been waiting quite a while to get. Everyone I saw on eBay and Amazon had it for price well over 50 bucks which is just absolutely ridiculous. I managed to find this one. One night I was tossing and turning in bed couldn't sleep I decided to browse eBay and I thought I'd check to see if they actually had it. The first listing on there was this one here $21 plus shipping and handling ended up coming out to about 30 bucks very very awesome. You got the green slime packaging with the safety stripes there at the bottom. You have the back of the package here Abby Yates, Aaron Gilbert, Gillian Holtzman, Patty Toland. Abby came with the arms of Rowan, Aaron Gilbert came with the legs of Rowan, Patty Toland came with the head and Gillian Holtzman came with the body. As you can imagine how frustrating it is having all of them except for the one that has the torso. So I've had all these little parts lying around for the better part of two years now but I managed to find it and now we're gonna cut into it and take a look at Gillian. All right so as you can see it's pretty much the same basic base body except she has no lower sleeves on her arms. Everything else is almost identical to the other ones aside from the head. Very cool figure, doesn't really look too much like Gillian Holtzman in the movie. Not a super good likeness of her but it's still good nonetheless. I kind of wish they would have gave her her little hand pistols like she had in the movie that come out from the bottom of her pack. That would have been very nice but they would have had to do a completely new mold of the pack for that. So I can understand that. Let's go ahead and take off the proton pack. You want to be very careful taking off the proton packs here. You got to put the arms backwards gently slide it off because it is a very soft rubbery plastic that could easily tear. And yeah she's got the same sculpt or the same articulation as the other figures. Her head is on a ball joint go up and down side to side all the way around. The head bob arms go up to about there. All right arms go up to about there. However if you turn them and go up all the way down about that far she has no shoulder or no bicep swivel. She does have an elbow swivel. She also has an elbow joint seems like a maybe a soft ratchet joint. However I would be careful because I can see as I move it the sides are wanting to pop out a little bit. She has a wrist swivel right there. Wastes a swivel.  They can raise up that far in the front which isn't really all that far at all. And about that much in back she has a thigh swivel. Ooh that's tight. Knee joint it's really tight. And an ankle joint ankle rocker. It doesn't go side to side but it does go front to back. As you can see her glasses are the yellow transparent or goggles whatever you want to call them. And her name tag only shows the last four letters of her name. Man because the collar flap is blocking the rest of it. There you have Ghostbusters trademark copyright 2016. 2016 Mattel. Very nice paint application. They could have maybe done her hair a little bit better made her look a little bit more Jillian-ish. And the proton pack is the exact same proton pack the other Answer the Call Ghostbusters have. And you can attach it, attach the proton wand to the pack just like that. There's peg right there and a hole right there. You just attach it. Not bad. Not bad at all. Now we're going to go and put the proton pack back on her. See if I can do it without breaking anything or tearing anything. Very gentle. Then we just put proton wand in her hand like that. Let's go. And there you have it guys from the 2016 Answer the Call Ghostbusters toy line by Mattel Jillian Holtzman action figure. I'm Shannon from Come Again TV the only place on YouTube where I'll geek culture collides. If you enjoyed this video make sure you hit the subscribe button so you don't miss out on future videos. And we'll see you next time my friends. Take care. This is Come Again TV


What's going on everyone? Sam here, late in sports cards, ripping one box, a 2022 Canadian Peckable WWE hobby for Sean D. So good luck here Sean man, I am so excited to see what's gonna be in this. This is gonna be absolutely wild. I love this product. Anything impeccable is always so much fun. Yeah, I would say they're pretty popular Ryan, especially now too. Like they started, the community started making more. So like, yeah, I've had Prism come out, Select come out, all kinds of stuff like that. Nice. Looks like we got a bar card in this one. We'll take that for sure. Empty. We'll open that potential bar card last. Yes. No. Yes. Mansour, 21 out of 35 on the gold base card. Again, just so you guys know, that is part of the design down there. It looks like the corner is a little nicked, but literally that's the design of impeccable this year. Very strange, but whatever. That's Sami Zayn, 49 out of 99. Fake design. Now that is a, trust me, that's a real design. We had to deal with this all throughout basketball right there. Empeccable WW, we haven't met 500 a box, 500 a box. Commander Aziz, 32 out of 99. Nice one there, Commander Aziz. We've got for SmackDown, Elegance, Patch Auto 25 out of 25. We've got Gunther. Nice Gunther there, which is actually cool.  We've got Superstar, Warn slash use material. Nice one there, we'll take a Gunther. Next one's really sick, we've got another Elegance, Patch Auto. We've got Drew McIntyre, 2 out of 99. There you go, congrats Sean, that's really cool. I love Drew McIntyre, very nice one there. We've got a Don Morocco, illustrious ink, 31 out of 99. Nice one there, 31 out of 99. He has a weapon, yeah he does, he's got like a dagger basically. Very cool Don Morocco. Next up we've got out of NXT, that is going to be Lash Legend. 23 out of 25, this next one's so sick. This next one's so sick. Lash Legend on the Superstar autograph. Again, nice low number there, 23 out of 25. Nice way to end it, we've got 54 out of 99 on the Stainless Steel. Seth freaking Rollins, boom! There you go Sean, congrats buddy. That is so awesome, so freaking cool. Again, that is 54 out of 99 on the Seth freaking Rollins. We will take that for sure, we'll get that in a mag for you after the break is over Sean. Stainless Stars. Alright, let's do your bar card now, this one's pretty heavy, so let's see a big name. That's going to be Mandy Rose, one Troy ounce of Fine Silver, 27 out of 35. Very nice one there to end it for, at least you got the bar card, only one per case, so very nice. Love pulling a bar card. There you go Sean, that'll do it for your break Sean, thanks again for jumping in. Appreciate it, love ripping the WWE stuff. We'll get those right out to you my man.


Welcome to MOOC course on Introduction to Proteogenomics. Today we are going to talk about next generation sequencing, sequencing by synthesis. In this session Dr. Arthi Desai will give you a glimpse of base space sequence hub which is Illumina cloud computing environment to analyze the sequencing dataset. She will mainly focus on how to search in public data, import data in personal dashboard and how to create your own project. Dr. Arthi will also explain you about the sharing of data among users and how this hub is playing a big role in collaborative platforms. Finally, she will also show you how to launch an analysis in base space with the help of multiple software already available there. So let us welcome Dr. Arthi for today's lecture. So now we can actually move on to... Clicking on the link will take you to the base space login page. Login or create an account by clicking register now. This will give you instant access. The first time you log in to base space you may need to read and accept the user agreement. After logging in you will be taken to the Tuma normal base space project page. A project is a container for various analysis files. These include samples which are fast queues that result from runs and output files from running analysis. These are stored under app sessions. In contrast a run in base space corresponds to data from sequencing a single flow cell on an instrument. The project overview page contains a brief description of the dataset. You can navigate around using the quick launch buttons either using the buttons on the left tab using the names or the direct links. These all link to the same place. The collaborators button indicates who the run has been shared with. If you are the owner of a run or project you can change the name and transfer runs or projects to collaborators. The share button allows you to share your projects. Note that we cannot share this project since we are not the project owner. You can also access the base space blog from this page. The blog provides useful updates and information on base space. There are three samples for this dataset. HCC1187C is the cancer sample from the breast ductal carcinoma cell line. HCC1187BL is the matching lymphoblastoid cell line from the same individual representing a normal sample. HCC1187Somatic represents the subtracted data. The normal and tumor samples are compared and matching sequences removed leaving only the tumor normal differences. Inside the samples hyperlinks you will find FASTQ files. These will usually be full FASTQ files but in this case empty FASTQ files were uploaded as placeholders. You will also find information about the samples including the read length, the total number of reads and whether this was a single read or paired end run. Inside the app sessions there is a folder for each analysis performed. Here they are named Illumina's Uploader as they were uploaded directly by Illumina. The naming convention should be more informative in the future but here the sample names correspond to the app sessions so you can use these sample names as a guide. Clicking on the hyperlinks will also show you the folder name. Inside the cancer and bloodline folders you will find BAM files, VCF and Genome VCF files and there will usually be a summary report for the analysis. Inside the somatic folder are VCF files from subtracted data. These show the variance between the tumor normal data. There is also the somatic summary report. You can open the somatic summary report within BaseBase or download it directly to your computer.  Go to public data in here search for true site cancer. Go to public data here true site cancer it is there on your screen okay true site TRU SIGHT cancer on the second project that is the MiniSeq true site cancer. So do you have this MiniSeq true site cancer project you all saw that okay click on that when you click on that it will expand and you will see two options there one is run and there is project what I want us to do is to import the project so click on import. So when you click on import did you guys see import or shall I go back yeah everybody with me so far okay. So when you click on import you will get this pop up that says you know I want to share a project with you accept it so once you accept it you should see it in your notifications that you now have this project MiniSeq true site cancer. So what I want you to do is click on that you know click on this notification that says MiniSeq true site cancer so click just click on that once you do go to the samples tab there is a tab here that says samples go to the samples tab take one of the samples this guy the second last one which is HD 701 rep 1 so these are this is a reference sample from Horizon it is a company that provides reference RNA DNA samples so that you can check the quality of the data quality of the panels that you have developed and so on and so forth. So it is essentially QC data right but it is very easy to look at which is why I wanted to show this to you so you select this yeah so do copy and do you guys have any projects no okay so sorry my bad I missed a step let us exit this cancel let us cancel this okay go back go back to projects go back to projects okay let us exit that for a moment go back to projects is everybody in projects yeah so just create a project you know name it whatever you guys want to name it just going to call this IITB demo very creative so you can name the project whichever way you want so it will create a project do you now have a project yes okay now let us go back to the dashboard let us go back to the dashboard now again click on the mini-seq true site cancer project go to samples last sorry okay I will go step by step so you created a project no so okay do you see this projects yeah if you click on that there is a like a file icon which says new project okay so click on that and give a name to the project everybody has created the projects yeah okay so let us go back to the dashboard into the true site cancer project go to samples okay and go to this HD701 rep 1 that is the sample that we want to copy so if you scroll to your right on the top there is something that you see copy to that menu option that says copy to so you can copy this now you should have a project the one that you just created and copy there okay the reason we are doing this is I wanted to show you how data sharing works on base space right like I said this is not a very very critical function in the application the only thing we wanted to show you is how you can share data across different users because this is meant to be a collaborative platform if there are any public data sets that you want to analyze this is how you would import those data into your own workspace so that you can run your own analysis okay that really was the crux and what I what else I wanted to show you was how to launch an app okay so let's say now I am just for the sake of time going to copy this one particular file onto the project that I have just created let me go back to projects let's go back to projects in this whatever project that you have just created if you are able to copy the file copy that particular sample it should be there okay I think I have copied it twice so you see two times but if you have been able to create the project select the sample and copy it to the project that you created you should be able to see a sample in your project okay so what I wanted to show you now is launching an analysis once you have your data into base space how do you launch an analysis and I just said that there are multiple apps or applications that are available on base space so this particular data was generated for a panel known as a true site cancer okay this is a panel that is available from Illumina that is used yes so this data has been generated for a panel known as true site cancer that's available from Illumina it is a panel that is designed to detect germline mutations okay so what we are going to do now is once I select the sample that I want to run the analysis for I am going to click on this launch app everybody or at least most of you with me so far yeah you have a project but those of you who can see this select the file and click on launch app when you click on launch app the options that are available there are multiple apps that are available okay depending again on the analysis that you want to do okay because this is a cancer panel that you know we have done enrichment on I can choose an enrichment app okay now what this will do is you know it will run what is known as variant calling okay it will call mutations it will call insertion small indels okay and then I can select the enrichment app so what I did was I selected a file from my project I clicked on launch app from the launch app I selected the enrichment app because the data that I am using for the workshop today is from a cancer panel true site cancer and we want to run an app that will give me variant calls you know mutations and small insertion deletions okay and these are again third party apps see the whole idea behind base space is to make these apps which are traditionally you know developed as command line tools available to the end users because if they are in the command line form unless you know if you are if you are unless you are good at some level of scripting it is hard for you to use them and majority of the apps that are developed for engines data analysis are command line apps because they are developed by advanced bioinformaticians okay so there is an analysis name you know which is the name that you want a unique name that you want to give to your analysis you know that is again your call where do you want to save your results so you by default it will pick up the same project from which you have picked up the input data files if you want to save it somewhere else you can do so select samples so you can go to the project select any sample that you want to run select okay if there are BAM files BAM files are nothing but aligned data files okay that are generated by aligners so you can select that reference genome that you want to use for your analysis because if you remember from the video I showed you once you generate the read data in order for you to do analysis you have to map it back to the reference genome right and in this case we are dealing with human genome data not unknown species so we will have a reference genome against which we will map the data files and you can also choose the targeted panel so that was you so in this case as I told you this is a true site cancer data right so what I will do is I will choose this true site cancer version 1 region files so essentially this will define for the application what are the regions in which it should look for variant calls right so it will significantly shorten the time it takes for analysis otherwise the app may end up scanning the entire genome which may result in two things one is extremely long analysis time and second some of these because we have repetitive regions in the genome homologous regions in the genome you know it you may end up with random alignments which will give you false information right so the targeted the target files essentially help you in streamlining your analysis okay and now let us hope that it works check that I have actually done everything that I am supposed to okay I think there are more things if you continue scrolling down you will see that there are certain other options for example whether this is for germline or for somatic I told you earlier the true site cancer panel is meant for germline okay germline variant detection so that is what I will use we will we are going to really leave all the other parameters as default right there are many many options because most of these algorithms come with multiple options that you can use to tweak the way the analysis is performed and the kind of results that are reported we recommend for our new users basic users to use a default analysis as you become more comfortable with the data that you are handling you can play around with the parameter options and I am going to have done everything yep so I am going to launch the application so when I do this what the application should do is take the input files that I have given and start running the analysis this analysis will take a little bit of time you will get a small pop up accept it okay now you should have one more notification saying that you know you have accepted data from one of my colleagues let us look at yeah let us look at the first analysis so just click on it click on the first analysis you will see four analysis in the you know once you put in that address so this is essentially going to be the output of the analysis that we just started okay so this is the output of the analysis that we just started okay and what this is showing you is some metrics of the data that we have analyzed so you can see that for this particular sample more than 98% reads aligned back to the reference genome okay again which means majority of the data is usable more than 98% of the data read data that was generated for this particular sample is mapping back to the reference genome which means it is usable so it gives you information like read level enrichment base level enrichment target level enrichment so these are all really quality metrics that you want to use to make sure of two things one is that your read data is of high quality and B you have very very specific sequence data that has been generated in so if you see here the target level enrichment is close to 100% 99.77% which means that you have the sequences that you have are from your target region majority of the sequences that you have are from your target region if this number is low which means that you have off target sequence data in your file okay so there may be something wrong with the way the data was generated you know there may be something wrong in the way the library was prepared and so on and so forth so you can use this to make sure that your workflow was sort of you know error free so to speak what it will also give you is the number of SNBs structural variations okay sorry SNB is a single nucleotide variations or one base changes that were identified in this particular sample it will also give you the number of indels that is insertions and deletions that were called in this particular sample it gives you coverage summary and also the depth of coverage in the targeted region so what you see here on the horizontal axis is the depth of sequencing coverage so I think Mukesh talked about the x coverage right so when you are doing next generation sequencing you are actually sequencing just two minutes you are sequencing every base multiple times okay and then depending on the application you are running your depth of sequencing can be as low as 30x so as Mukesh mentioned for whole genome sequencing we are really looking at 30x sequencing for job for somatic mutations you may want to sequence as deep as 5000x 10000x depending on the frequency of the mutation right if it is a rare variant and you know in cancer samples especially if it is a heterogeneous tissue sample you may have to sequence deeply liquid biopsy is another example where you have to sequence deeply because you are really trying to identify those cancer DNA cell free or you know CTCs in your blood which will have you know DNA from your normal cells right so the depth of sequencing in this case is very high and the median fragment length and so on and so forth so you are really getting a lot of metrics you can also actually somewhere that is not shown here but see the specific mutations that are called so if you download some of this data you will be able to actually see the specific variations that are called you know we have looked at NGS data generated on the Illumina platform we have seen how we can share data amongst you know our collaborators run some analysis and looked at what the output you know may look like depending on the application that you have run since you all now have base space accounts there are many public data sets that are available right so if you go to the public data section on base space you will see that there are thousands of data sets that are available so you know you can look at those at your free time and you know reach out to us if you have any questions hope you guys found this session useful thank you I hope today's session by Dr. Aarti Desai was really informative where you learnt how base space can be used in sequencing analysis data sharing and how data output looks like she also showed that this platform is a collection of multiple applications and demonstrated you a single application that is variant calling she gave a detailed information of parameters launching of the app and how to run it I suggest you to play with other applications available there and you will find them really interesting as Dr. Desai also told you that you will learn more when you will play with the parameters of each application with new data set I'd like to mention you there are large amount of data set available which is publicly accessible on various portals such as the cancer genome atlas or TCGA and there are various ways one could download those data set and by using these tools try to understand and analyze the data so more and more you are playing with these tools and you are familiar with the software features you can then make use of large amount of public accessible data set from various large genome sequencing projects in the next supplementary lecture we will learn about iron torrent a bench top next generation sequencing technology by Dr. Atima Agarwal thank you


Hi, beautiful. I know so many of you suffer from hair loss, either from having COVID or maybe it's just hereditary. Hair loss can happen for so many reasons. But don't worry, it's not the end of the world and I am here to help you. But you must take some precautions in order to mitigate the problem before it gets worse. So today we're going shopping at my local drug store to find some products that will help you with your hair loss problem. So let's go. Okay, we have made it to Walgreens. Let's see what we have in the aisles. Also, I get really embarrassed recording in public. So if I look awkward, that's because I feel awkward and I hate every moment of it. Okay, so first I'm gonna look at brushes and we're gonna get probably a good little detangling brush check. We're gonna get a bore bristle brush. Okay, yeah, that's good. This is good. Perfect, we have a scalp brush thing. I don't know what these are called actually. A shampoo brush, I guess? Dead-free scrunchies.  I actually like the Batiste Dry Shampoo. I'm not gonna lie, it's kind of good. I'm just gonna get the original one. I think that's the one I like. I haven't used it in a bit, so put that in the bag. Let's get some cleansing shampoo that is very gentle for the hair. This one actually says it's for hair growth, so I'm gonna put that in the bag. Oh, they have a little salicylic acid shampoo. That is great for dandruff and it can be great for some hair loss issues. What else we got here? Okay, we got a silica bonnet. Cool, let's put that in. And a few other things. Oh, they have monoxidil here.  My social anxiety is peaking right now and I don't wanna do it anymore. So there are people staring at me and I need to go. I'll see you when I get home. We're back, let's go through the bag. So I think I got some good stuff and I think I'm really gonna help you out. It's been a while since I've shopped for anything a hair related in a drug store but I was kind of impressed at the selection. There's actually some really good products in there and some things I wanna show you to help with hair loss. We picked up a detangling brush. This baby is great, guys. They're so underrated, these detangling brushes. Oh my God, I remember their time where there weren't detangling brushes like this. They weren't so well known. I love the tangleteaser ones. I just feel like the bristles are really great. I feel like it's high quality and it's light and it's nice. It feels good in my hand, it's a good vibe. This really helps prevent excess hair loss because the bristles are nice and bendable and it's not gonna pull the hair out of your scalp. A lot of people will really roughly pull on their hair and it will come out. If you pull your hair really hard with a brush, especially when it's wet, it's fragile as sh** and very elastic. If you pull it when it's wet, it will come out of your scalp or it will break off really close to your scalp and you do not want that to happen. So we are gonna help that situation by starting at the ends of our hair and brushing up. And if there is really, really tangled parts of your hair, please do not try to get it out while your hair is wet. Allow your hair to air dry a bit and then work on the tangles. Or work on those tangles when your hair is 100% dry because your hair will not be as elastic and can take a lot more tugging. Usually hair that is thinning is very, very fragile and very fine. So you wanna make sure you're not putting extra tension on it and this is really gonna help the situation. Another one in our brush category is this Boar Bristle Brush. These bristles help distribute oils in your hair, allowing your hair to really soak in that sea bum which is so good for your hair and so good for hair growth. You wanna let those natural oils out. If you also wear your hair in point tails a lot, this helps prevent extra breakage by not pulling on your hair too much. These bristles are also really soft and really bendable. So you're not gonna put extra tension on your hair. You're gonna get that oil moving throughout and it's kinda just brushing the surface of your hair where a lot of brushes go really deep and you end up pulling on more hair than you actually need to. This is great for people like me who have a very, very fragile hairline and if you put any kind of, even like a detangling brush on it when it's dry, oh my God, it will pull, it will hurt and I will lose hair. I will have 10 hair strands on my brush. I would use something that made out of borer or a synthetic and borer mix, something like that to not put too much tension on my hairline. I love these scalp brushes for shampooing but I use them for so many different things. If you've never seen one of these before, this is what they look like.  Oh God, it gives me chills, I love it. This is great for when you're shampooing. It helps really clean up that scalp. If you have a clean scalp, it really helps with hair growth. That doesn't mean you should wash it every day. You should wash your scalp at some point because sometimes you can get buildup on your scalp, preventing your hair from growing. When you're shampooing your hair, sometimes your hair can snag on your nails if you have a sharp nail or a little bit of a hang nail situation going on. Also, your nails can be a bit too abrasive for your scalp. Your scalp is pretty sensitive and can't really take a lot of that aggressive movement. These soft bristles help you so that you're not putting any abrasions in your scalp or being too rough on it. You don't want to put little cuts in your scalp but it's just not good for it. You want to take care of that scalp. If you love your scalp, your scalp will love you back and produce more hair and it'll be a great relationship you guys have. This also stimulates your scalp's capillaries. This will also help with blood circulation and helps nourish your hair follicles with oxygen and nutrients. There was one point where I was using this twice a day and I was just going like this throughout the day. First of all, it feels so good and you can do it at any time while you're working at the office. Who cares if people judge you, whatever. It was helping my hair grow so much. Whoa, girl. I really need to do it again. I don't even know why I stopped but yes, these things are great. Let's get into hair products. Dry shampoo, Batiste. Ooh, this one is a cheap one but a good one. Like I said before, you should be washing your hair. You know, I recommend at least once a week. Two times a week is probably like the best of all if you're suffering from hair loss.  I think that is important but if you wanna prolong the life in between washes we all know a dry shampoo is great for that. Just use it like once or twice between washes though. Do not use it too much. You don't wanna overdo it because what you can do is put too much product in your scalp. If you put way too much powder and product in your scalp and don't wash your hair for like two weeks your hair follicle is not gonna be able to grow out of that. Let's keep those impurities to a minimum and keep it nice and clean for as long as possible without putting an overload of dry shampoo. But dry shampoo is still great and still can help strengthen your hair fiber by letting that hair sit in those natural oils for a little bit longer in between washes. I also found this product called Thicker Fuller Hair. It seems to have really great ingredients and a good idea behind it. I've never actually used it. It has a lot of really great oils in it that are great for your scalp and a cleansing shampoo can be a great option for people with fine or thinning hair because it's not gonna overly strip your hair of its natural nutrients but it is going to get it clean. Switching to something like a cleansing shampoo can be great for people with hair loss so you're not taking those nutrients out that are so good for your hair and for promoting hair growth. This also prevents your hair from getting too dry. If you have very, very dry hair try something like this or try something like a hydrating shampoo because very dry hair can often split and become broken and brittle and we don't want that. This is one that I actually was like should I pick this up or should I not? I find this very interesting though and I feel like a lot of people don't realize that dandruff, psoriasis and eczema on this scalp can actually prevent your hair from growing properly in severe cases. So if you have any kind of dry scalp consider something like salicylic acid shampoo. You can get skin buildup on your scalp. This unclogs your hair follicle and prevents oil buildup. So this is great thing to use once in a while just to make sure your scalp is free of all impurities and really clean. That way you can grow lustrous, shiny, thick, healthy hair. Let's talk about some accessories, shall we? This one is kind of an obvious one. I'll go through this quick. This is a microfiber towel. If you are seriously thinning and a lot of hair is coming out when you dry it on a towel please use a microfiber towel. So good to just be really, really gentle on your hair and these will prevent any excess hair loss due to rough towels and going like this. Don't go like this with a towel either. If you have hair loss just blot the hair. That will be great for you. I have a love-hate relationship with these things. I don't know what that says. Scoon, scuncy spirals, scrunchy, I don't know. I think they're absolutely hideous but if you're going to the gym or like something casual, sure, why not? I know that these really are nice on the hair especially the ones that are not matte like the shiny ones. The glossier material does not pull on your hair at all. These are just great for people with fine thinning hair because they don't pull. They're not gonna add any more stress on your hair than needed and it's just a great little item so that your hair stays intact and doesn't get pulled on at all throughout the day even if you're wearing it in a ponytail because ponytails can be really stressful for the hair. Let's talk about a satin bonnet. I see a lot of people getting satin pillowcases or silk pillowcases and like, I don't like the way they look so for me like a satin bonnet is just so much better. Also, when you're sleeping, if you turn side to side and toss around all the time you know that you wake up with matted hair underneath your head or on top and it gets really wild and people often comb it and brush it really, really hard in the morning. It's not good. Let's prevent that because what can be happening is your hair can literally be snapping while you're sleeping, okay? People with thinning hair often don't have strong hair so if you want that hair to stay intact let's make sure we are protecting our hair while we sleep. That way it's not all tangled in the morning and we have to rip it out with a brush. Not a good idea. This also just helps your style last longer because you have it all in this bonnet and it's staying nice and fresh all through the night. This soft material makes sure your hair doesn't get too tangled while you're sleeping. They're just a great useful tool and super easy to use and a super easy way to make sure your hair is not being pulled out. We have a little medication moment. Ooh, ah. This is a minoxidil treatment, 2%. Minoxidil can be really effective for a lot of people but not everybody, okay? It does not work on everybody. I am the person who doesn't really do anything on but this can really affect your hair growth in a great way. It basically shortens the resting phase of your hair and speeds up the process of growing new follicles. You do have to use this every single day and you have to put a red on your scalp. It's a little annoying but it can be really effective and this has been studied heavily and there's been clinical trials. We do know it's actually effective on a lot of people so if you're suffering from severe hair loss or a thinning hairline, this can be really great for you but beware that if you stop using it the effects will go away. So you must routinely use this forever if you wanna maintain the results. And lastly, we have a multivitamin. However, however, first of all I wanna say this is one that you need to consult with your doctor about and get tests for. I just picked up a multivitamin for an example of something you could take but you could be definitely deficient in vitamins or you could have hormonal problems going on.  I know a lot of people out there, maybe you do not have a proper diet or they're getting the nutrients they need in order to help their hair grow properly and then you are deficient in some vitamin, it really won't be a good combination. So let's get the vitamins we need and these are easy to find at the drugstore obviously and it's a good thing to have for growing your hair. I would say that was a successful mission to the drugstore and we found a lot of great items. I hope that helps you and helps prevent more hair loss and helps you recover some of the already lost hair. I know how much hair means to so many people, especially me, so please do yourself a favor and adopt a healthy hair care routine with all the necessary items to prevent excess hair loss and regrow new hair. I hope I helped. Make sure you guys check out my hair care or my hair color line. It is called X-Mondo. You can go to X-MondoHair.com or click the link right below to check out our products. And that is all for today guys. Thank you so much for watching. Don't forget to live your extra life and I'll see you next time. Bye.


What's up everybody and welcome back here to the channel. I'm evil rabbit We are here on a set of Corsa in content manager We can take a look at my wheel settings and get some sliding and some random lobbies But before we get into that something I'm very excited to talk to you guys about I've partnered up with Teespring to bring you guys the ability to pick up some evil rabbit merch yourself So if you guys want to know where to get it the link will be in the description box below But also you can go to my youtube page and click on the evil rabbit merch But it'll take you right to the merch store We got the evil rabbit logo on t-shirts tanks hoodies and a black logo on the white tea and See you on the track little slogan on the back Super excited to have this available for you guys and have it up and available for you guys purchase if you want to Support more merch is gonna be coming to this store big Thank you, you know to the guys at Teespring for being able to hook me up with this merch store So that you guys can pick up some evil rabbit merch of your own if you would like to rep the logo So like I said more merch will be coming here very soon Definitely would appreciate the support from you guys if you guys want to rock the logo And if you do purchase yourself some of the evil rabbit swag Make sure you guys tag me on Instagram at evil rabbit 07 that way I can see you guys with it So once again, you guys can get it in the link in the description box below Or you guys can click on a little evil rabbit merch on my youtube channel itself It'll take you right there, and you guys can do you know pick some up yourself that does ship globally So if you guys are from the UK or anything like that you guys can still get it So super excited that uh Teespring is now going to be handling merch and stuff like that for me So definitely a big thing for me and you know a big thing for you guys We guys want to pick up some merch yourself, so let's get back to wheel settings So a lot of you guys been asking what my will settings are for my fan attack. It's very actually simple I do have my wheel setting gain at 60% I have zero filter percenting minimum force feedback at zero I have curb effect road effect slip effect ABS effects all of its turned off and I do have you know central boost gain at 10 and the Central boost gain zero and the range at 10. Sorry. I apologize for that Because the reason I have all this stuff turned off is because my fan attack is Trying to counteract it so as for my fan attack settings You guys can probably see it on my S1 right there I'm gonna try to go behind this so you guys can see it for sensitivity. We have it on authentic force feedback We have 100% SHO we have it 100 ABS we have it 45. That's just personal preference for the vibration of my pedal DRL, which is a drift setting. We have it negative one to give the wheel a little bit more Friction when it's rotating back and forth. I found it works best for me and Then for I believe that was a fo r right. Oh, no go back to a hundred. We're at a hundred SPR we're at a hundred DPR right a hundred breaking force. We're at 50 I used to have it at 51% FE I at a hundred and those are my wheel settings in my actual fan attack so These are the settings that I'm running. We're gonna go try and get into a random lobby here I was looking at this need for speed 2005 track Because it looks pretty fun and it's got the acd, you know cars in there Which I do have a lot of fun driving these acd's so we're gonna see if we can maybe grab some tans of some Randoms here, so make sure you guys follow me on Instagram and Twitter all which found the description box below as well as the link to the Merch site super excited to have that Merch site now available Running 100% like I said more merch will be coming so you guys want to pick some up check it out in the link description So we're gonna join in and we're gonna see if we can't get into a lobby We got to get the Wheel cam and everything turned on there we go will cam pedal cam all turned on ready to go. I Thought I almost unplugged my microphone there for a second gonna move my seat forward Advantage of seat sliders Alright, so we got some people on the track. We're going to adjust our tires. We're gonna drop these tires down to 22 PSI and we're just gonna send it Turn the lights on make sure we're at 100% boost So this is a pretty fun track. I'm not actually sure where I got it downloaded from If you find a lobby, you can click the little search button and download it through there I believe you can find a link through there It's probably one of the first tracks ever went on to with these ACD cars And I was able to grab some serious doors with these guys So we're gonna try and see if we can maybe grab doors here today. It's a fun little track I mean guys were playing need for speed. I believe this is actually One of the tracks that was in the game You want to go let's do this. Let's do some tandems Falcon tandems, I don't think he wants to I don't think so go wants to go He's not really So going and I'm not sure there's anybody else on the track right now down to the third there we go Just a little wall grace little booty bump we good If you guys didn't check out the tandem video with fernan 820 and I at OSW make sure you check that out last video that was uploaded before this one Definitely was grabbing some serious doors with fernan having a lot of fun definitely getting way more comfortable in my chasing and It shows in that video and it goes just to the attest of see time and practice doing all this with a bum finger is well a little bit of a bummer and I'm super excited that this will be coming off soon. I see somebody ripping out of the pits We can be able to grab some tandems Oh, we got two cars behind us. All right now.  Oh Tandems with randoms I kind of almost biffed that I'm gonna pop off sideways here. Let these guys go let these guys go up Kind of cuts a track a little bit So it's definitely fun hopping into random lobbies and trying to you know tandem with some randoms and stuff like that But you can don't always find some of the smoothest drivers So I've been noticing that my Tandems have been getting better following some very solid drivers I do have my discord channel almost fully set up So I will be doing the discord here very soon. Oh Those guys just died We'll be doing the discord here very soon so that I can start running some public lobbies and public sessions With the fact that I can run servers to get some more tandems with some other people and have some fun And you know be able to talk to you guys in this session with my discord server Fern and I were using it testing it out me. Everything was good. Oh my god. That dude just surged my door Double initiation a little bit. I Don't know what this dude's at Apparently he wants to follow me. So we're just gonna run maybe run one more run In the lead position and maybe try and slot back into chase Little bit too greasy on that wall. Well, now I know a lot of you guys. Oh my car went up first What? Okay, that dude doesn't have to lay into me. Oh my god So I know a lot of you guys are saying oh man driving a third person Well most of the time I spend driving in first person Sometimes I spend driving a third person, but I bring the you know First person I bring the third person view to you guys to make it more entertaining for you guys to watch and for you to see more Rather than just being in cockpit with real head motion and that will change soon once oculus becomes a thing Then definitely we'll be getting full first person oculus content Trying to back that in there apparently So like I said, I'm not entirely sure where I got this track down on from I know a lot of you guys are probably gonna ask for a description Our link in the description. I don't know where this track came from so I can't really tell you So I do apologize for that But if you do find the server on content manager should be able to click the little You know magnifying glass next to it. It should be able to bring you up to where it is So we're gonna try and wait for this e46. I think E92 Canadian e92. I Don't these cars can I I think he's just Canadian Here we go So doing tandems with Randoms that are not always super fluent is also a very good training tool I found because it teaches me to basically tandem with anybody because you know competing in the Boshota series Which you know round one didn't qualify unfortunately Kind of best my qualifying runs if you guys watched it alive So round two won't be until next month beginning of the year But tandeming with randoms in some cars is definitely very good for me to learn to how to tandem behind other people that are not maybe as fluent and I need to be able to tandem with people that oh Maybe aren't as fluent because not everybody that's competing is always super fluent all the time and You know, you definitely need to practice with you know, very good drivers. Luckily. I have a good team You know backing me and you know always willing to help me get some tandems you know the usual suspect guys and they're very fluid very solid drivers and Fernan as you saw in the later video before this one He's definitely a very solid driver as well So we get a couple good drivers that I drive with as well as I like going into finding randoms because you know I've already found a couple of you guys You know that are subscribed to the channel in some lobbies So it was definitely fun to get doors with you guys and definitely going to be doing more Lobbies with you guys once I get my discord and everything fully running so now Gonna see if maybe we can find a different lobby right now So unfortunately, I'm not really finding any good servers right now But I did want to just bring you guys my wheel settings So as I know a lot you've been asking and talk to you guys about the merch store So make sure you follow me on all social media and as always I like thank you guys for watching. I'm evil rabbit I'll see you guys on the track


Hello friends, I am Dr. Rajneesh Sardana. I am Head of Cardiology at the Manipal Heart Institute. Today we are going to talk about chest pain when to worry. See, we get a lot of patients with chest pain in the young people, in the middle ages, in the elderly. We all recognize that the chest pain in the elderly is usually worrisome, but that does not mean a young person chest pain can be ignored. So, I would like to highlight what is the character of a cardiac chest pain. See, when we say chest pain, cardiac, we call it an angina. Angina is what? It's not a pain. We keep calling it a pain, but it's not a pain. The usual feeling in the heaviness, tightness or squeeze feeling in the chest where you feel everything is constricting and something is stuck. It may go to the arm, may not go to the arm. Usually it will last 3 to 4 minutes and then ease up. The same pain when it becomes more severe, it becomes a much more difficult person associated with breathlessness, perspiration, that can indicate a harder thing. So, I will again say, when we call a chest pain, it's never a pain. It's more of a feeling of squeezing, tightness, heaviness, pressure like feeling which will last few minutes. Usually it will come on exertion and get relieved on rest. There are patients who got chest pain and they said that when I walk, I feel better. Almost certainly if the pain is getting relieved by walking on exertion, it is non-cardiac. Pain which is pinprick, particular spot where it just pricks and goes, it's never a cardiac. So, these are markers which you can probably not get along, but there are others. Again, then we also look at it in the perspective of the risk factor. So, if anybody is a smoker, anybody who is a diabetic, the symptoms can be much lesser and they can be a subtle symptom of some pressure heaviness. The last thing I'd like to say is that a lot of patients when they start feeling that there is something stuck, like a gas feeling because they ate something. Let me tell you that the commonest mistake people make is when they keep calling it gas, gas stuck and they start burping and they feel better and they say, okay, it's something I ate. Why would a person who's never had acidity suddenly one day have gas? So remember, if there's anything that you feel is stuck, pressure is not allowing you to move to do certain things out of the blue.  So, cardiac pain is not a pain, it is more about the sensation of feeling tightness. Thank you so much.


e've got a couple fillers for each break, and then some full spots, I hope we can do it. I hope we don't leave money on the table after getting this far. I, we knew it was gonna be a challenge. That's why it wasn't just a one night thing. We made sure that it was a three night situation. So hopefully that third night is exactly what we need. Nice, Ed looking ahead to Friday. Love to see it snags the boys, the cowboys and flaws collegiate one, two, three, four. Pre-orders have been pretty brisk for that, ladies and gentlemen. And Ed had the cowboys in absolute as well. Nice, some good pre-order in there, ladies. Yeah, even if, even if you don't think a break, I mean, I still believe we can knock out a couple filler breaks here and there. I definitely think we can do it. If not, if you don't believe that, don't buy spots there. At least pre-order, lock in your team. I know these breaks are on Friday, but if you see your team, get your team. Now a lot of times, random teams, you know, I suppose I personally don't like this strategy, but yes, you can wait until it gets closer for random team breaks. But for pick your team breaks, there's only one of your team.  I don't know how many times, you know, people have waited too long. You know, I'm trying to help you out. I'm trying to help you put yourself into a better position to get better hits, the best hits.  I was gonna say, I don't know. I've heard a lot of times people going, man, I wish I would've got that team. I had the money, I wasn't, you know, I'll just wait until the next day and I thought I was still gonna be there. My team was gone and in that particular break, the monster hit popped for the team that I wanted, you know, doubling down on the sad times there. I don't want you to have sad times, ladies and gentlemen. I want you to have good times. And part of that is putting yourself in the best position to succeed and the best position to succeed is just getting your teams straight up. That's right, help me, help you. That's all I'm trying to do. Just trying to help the people, you know? Just trying to help the people enjoy their break experience better. Putting you, trying to put you in winning positions. Look at those winning positions. What's going on in the sports world? All right, so we don't have football until Thursday. Tomorrow's Wednesday, so not for a couple of days. What's happening? Basketball on Wednesday? So that's a ESPN night, right? NBA TV. Oh, it's an NBA TV night. Trailblazers, Lakers. See how that works out. So a lot of hoops. And then we've got, yeah. How about that USA game earlier today? That was pretty wild. And Jilo, I missed, I forgot to respond to Jilo's comment, but he was saying how nuts that game was, especially towards the end, right? Christian Polixx scored that wild goal which he heard himself in the 38th minute just before the end of the first half. And then it was just furious defending. A lot of back and forth, it was pretty crazy. England won comfortably. Netherlands won comfortably. Senegal won as well. So the group stages are wrapping up. Australia, Denmark, Tunisia, France, Poland, Argentina. That's gonna be a big game. Saudi Arabia, Mexico. I'll have to make some plays there. See how my picks did today. I did get one right here. What's the path for L3 is just winning in? And they're having a hard time scoring goals. Mexico win, Poland wins, and then they're in. They have a hard time scoring goals. Like if Christian Polixx says he's gonna play on Saturday, I think, whenever that knockout round is, but like Mexico, the US have seemed to have a little hard time getting goals. If not more, I think it's maybe even like four nil and like a draw scenario between Poland and Argentina. And again, Mexico has had a hard time scoring goals. They haven't scored a goal this tournament. I wanna say it even goes back to the last time they're in the World Cup. I think maybe since this match, dating back to 2018, so they haven't scored a goal in like their last handful of matches in a World Cup competition. That's not good. You gotta get some goals on there. They have to get Chicharito out there. I'm not sure why he's not. I know why he's not there, but. Yeah, that's good for the hobby. Let's get some more Prism World Cup. We're gonna start to see, it's still pretty early, but we're gonna start to see some separation on some of these World Cup stats right here, right? By the time all these games are over, by the time all the third games are played, we're gonna see what teams are moving on, right? France is gonna move on. That's good for Kylian Mbappe, Golden Boot, right? Is Ecuador moving on? I forget. Portugal, Lionel Messi, Gopko, if Gopko wins Goppo, if he gets, Netherlands are moving on. You know, he's gonna get some more opportunities to get into Golden Boot situations. Brazil's Rashalison, Bukai Osaka, that'd be good. No say. Because it's gonna be interesting now, because now it's round three, so. There has to be some that have zero two, but maybe it's only Mexico. Tunisia has zero goals, but they don't have a shot. So let's just say Mexico and Tunisia. Can you believe Uruguay has zero goals? Isn't that kind of crazy? Let's just say it's three, then who would win that? It'd be that by the goal differentials they gave up, right? It'd be by this. Just to win whoever wins. It'd be this goal differential first. Yeah, sometimes. So like Tunisia gave up like so many already, right? Correct, so no, they're minus one. They're minus one, okay, that's not bad. But like Costa Rica, for example, they're on a minus six goal differential, but they have three points, right? Yeah, but they would move on though. So if they, if, what's their next match? Oh, I guess it's goal difference, it's not how many goals they score. No, correct, it's different, it's GD, not G. That makes sense then. So like for Costa Rica, they play Germany, right? So if they happen to, like if, for example, if they get tied with Japan or Spain or whatever after their goal differential, like let's say they draw and Japan draws, so then they're both on four points, Japan moves on because their goal differential is zero. Yeah. No, what I'm saying for our giveaway though, for the worst, for the least amount of goals scored, would it go to people that have zero and then it'd be going to goal differential after that? It's goal differential first, are you mean tie breakers? Yeah, that's what I'm saying, because like right, our one of our giveaways was the least amount of goals scored in the World Cup. Oh, for our giveaways? Yeah, yeah. So like, what if there's three teams that have zero goals? Then it would go goal differential. No, then we ran, we just randomized. That's what I figured. That's what I figured. Right, so if your team scores the least amount of goals during the group stage, you can win a case, a $1100 case of a leaf process. Hey, Mexico's still in it. So like zero goals, so it's still, so it's Mexico, Tunisia, Uruguay. And if it stays like that, or if they only score one goal each, yeah, then it'll be a randomizer. Yeah, if there's a tie, it'll be randomized between the people in the tie. So for the purpose of the promo, listen, there you go, Daniel Smith, you're still in on a case. Daniel, you're still in on a case. Carl, with Uruguay, you're still in on a case. Lowest number of goals scored in the tournament. Dannosaurus, what's going on? Nice. Well, listen, Dan, if you're lucky enough to get some hits from us, and if you wanna pick them up instead, definitely let us know. You can hit that contact button and we can put you on a local pickup list. So I don't know how far down the freeway you are, but if you're close enough, we can arrange weekly pickups or whenever you got time, kinda pickups. So we can definitely arrange that. Or we can still ship them to you, shipping's free. Hopefully we'll have stuff to give you, fingers crossed. That's the idea. That is the idea. Well, gang, it looks like last order I got was 20 minutes ago and that was for a pre-order. Last order I got for a break that we can do tonight. I think was many minutes ago. You're in the Long Beach area. Yeah, there you go, yeah. So if fingers crossed, knock on wood, but if we get like a one-of-one or a super fracter or something crazy like that, and yeah, if you're like, hey, can I come by tomorrow or this weekend to pick it up, absolutely. The only days we are closed are Sundays in terms of pickups, but Monday through Friday we're open and then for, you can see right here on your screen, on for the remainder of the year, so pretty much for the month of December, we'll be open to 8 p.m. on Fridays and Saturdays. Monday through Saturday we're usually 11 to six, but for Fridays and Saturdays, and close on Sundays, but live stream still on Sunday though. We still live stream on Sunday, just the shop is closed. But Fridays and Saturdays we'll be open a couple extra hours. So which might work for like let's, if you want to pick up a big hit after work or something like that, you don't have to get here by six, there's a couple extra hours. All right, but thank you. Thanks for discovering us. Well, Liza, I think that's it for me. We're about a little under 10 minutes away from the top of the hour. We're always down to go later if breaks are filling up, but it looks like it slowed down a little bit. So I think we might wrap it up a little bit on the early side. No harm, no foul. Then we can live to break another day. All right, well, Dan, good luck with the break. Appreciate you getting in. I don't know, Rex may still be listening in the background as he's working. Rex, Edgar, Logan maybe, anyone who's still up and awake. Thanks for hanging out with us. Yeah, appreciate it, Dan. I'm sure we gotta do like a hobby a day. Keeps the doctor away, I think, right? A hobby case a day. Once we get that down to maybe closer to single digits, we'll probably create some sort of filler for it to help get it across the finish line, but we'll make it work. All right, so everyone, thank you for hanging out with us, for breaking with us. We had a really solid day today. I think we're gonna have another solid day tomorrow. Hopefully we finish off this break credit promo tomorrow as well, tomorrow's the deadline. So hopefully we can get these couple fillers, the full spots. Hopefully we can get it all done. All right, see ya, Rex. Have a good one, man. Have a good rest of your night. And that's it for me. I'll be back three o'clock, Pacific, six o'clock Eastern, and we'll do this craziness all over again. Thanks, everybody. Have a great night.


So I told myself I was going to avoid this topic I've seen so much of YouTube and the internet talking about it, but It is something that potentially could affect us here at Nintendo Prime down the line So we're going to just briefly go over it PewDiePie you guys Probably all know who he is. He's the most popular youtuber on YouTube by a pretty wide margin His videos on several indie games have helped indie games blow up. He's crazy He's really popular with younger people, but I've even enjoyed some of his content over the years even enjoyed some of it just this year and I even was kind of sort of on his side when the Wall Street Journal was coming after him Because I felt like they took everything out of context that doesn't mean that he's perfect and this week he did something that is hard to forgive and He used the n-word During a live stream. I'm not even gonna show the video clip or anything. It's out there if you want to look at PewDiePie says the n-word you're gonna find clips of it both edited and unedited and That's pretty crappy of PewDiePie to do that it definitely and the way that he did it kind of suggests that it's just part of his regular vocabulary and He doesn't either understand or care about the context that word Hurts people but It's not so much about the usage of that word the One of the main people behind Firewatch put up some tweets about how he is issuing a DMCA takedown of all of PewDiePie's Firewatch videos, which are about 18 months old now and That's what we're gonna talk about today is a developer coming after a youtuber to DMCA takedown videos that feature their game just because They are trying to be PC basically They're trying to be a social justice warrior, which I totally respect the stance of what being against what PewDiePie did But here's a problem and this was brought up by the youtuber lawful masses with Leonard French He's a he's a real-life lawyer and he examined this and it turns out that Firewatch the makers behind it put themselves in a pretty sticky situation by issuing a DMCA takedown And it's only obviously an issue if PewDiePie decides to fight it and because on their website They specifically say in the FAQ section that anyone is allowed to live stream their game Anyone is allowed to profit off of live streaming their games. They specifically grant global permission for anybody to Livestream their game and they do not put in in in that global permission any Restrictions on how you do it so they're not saying you have to be a good person They're not saying you you know that there's nothing to say you can't be racist. You can't be this you can't there's no restrictions on How people are able to live stream based on what they have specifically on their website So because on their website it literally says you anyone can live stream and make money off it without restrictions That means that they kind of forfeited their rights to DMC takedown Anyone DMCA takedown like they literally cannot do it to anyone because they already gave everyone permission now The exception of course is if when they gave You know certain streamers free copies of games if they included An agreement with that free copy like if you get a free copy of this game It's kind of like an NDA when Nintendo you know makes you sign an NDA when you get review copies of games Which I've had to deal with back in the day back in my Zeldin former days So if you get safe say they sent me a copy of the game in Exchange for that copy of the game. I might have to agree to certain terms of certain terms might be that while I stream the game I can't say anything negative about the developers. Those are the kind of things. I don't like I don't like Agreements that make you force you to stay positive about a game You might not be having a positive experience with so I would never accept anything like that, but you have You have have sometimes they try to do that other times they will try to you know Hey, we'll give you a free copy of the game, but just don't be racist don't say anything sexual Don't dress in a certain way they can include any legal stipulations They want in exchange for that game because they are giving you something and in return you're agreeing To their terms of use for that something now obviously, you know, they cannot see avoid all that just say We don't want a free copy of your game, but wait till your game comes out. We'll buy it ourselves And then they don't have any of those restrictions but the person who's trying to take down the the PewDiePie fire watch videos Essentially on Twitter said every single person who live streams is technically Breaking copyright law which is completely 100% factual Most developers just leave it alone because they do make money off of those streams It gets people interested in the games and then they go out and buy them But as we found out what people like digital revolver who like went after Jim Sterling Because of his negative viewpoints on their games and made this whole giant pub up Jim Sterling won that lawsuit They he was free to use Their gameplay clips as much as he wanted as long as you know, he did something original with it which is original thing was his commentary and That is that that's where the streamers actually usually stand in fair use because of the Commentary value, but they still that being said People can make it illegal to stream your game And usually when they make it illegal, they're just talking about monetization wise as an example Nintendo could literally DMC a takedown every video I have that has any Nintendo published content in it, but Instead of doing that they just demonetize the videos Because they recognize there's still a value in those videos being up and getting eyeballs Unfortunately, they de-incentivize people from putting those videos up by taking away the money from them But whatever that's that's a conversation for a different day So you end up in this lawsuit here that they're apparently Could happen because they're gonna file a DMCA takedowns. I'm assuming through YouTube's automated process and the videos will get taken down and if PewDiePie wants to fight it unless he cites a separate agreement That dealt with his behavior and it's gonna be really hard because this behavior didn't happen while he was streaming their game It's Likely that if PewDiePie wants to fight it, he's going to win and that's what this lawyer pointed out when he talked about it He's like look it doesn't matter if you condemn the behavior the DMCA takedown Is basically they forfeited the rights to do that and It's it's going to be interesting if this does go to court because if For this does go to court and if you know despite all the evidence out there and lawyers out there can just say look You know, it's really easy to make the case for PewDiePie and not You know be able to fight back It's one of those things that even if it's easy to do that that doesn't guarantee That's what the result will be now. I'm not a lawyer my my fiance is mom's a lawyer She deals more with employment, but it's it's one of those things where they can They could win that let's just put it that way the the people behind Firewatch could win And if they do win and it's not like I care, you know if PewDiePie loses revenue and has things demonetized, but if they if they DMCA claim his videos PewDiePie fights the suit takes him to court and The people behind Firewatch win despite the fact that they clearly have evidence of forfeiting the rights to even do a DMCA takedown They've even forfeit the rights to demonetize videos if I'm being honest they If they win it sets a precedent a bad precedent as we saw with you know, the folks that went after Jim Sterling that well, if that's the case any game pay clip of any game if the person doesn't like you they could just DMCA claim your video and get rid of it and That's a problem. That's going to affect critique. That's going to affect let's players That's going to affect live streamers It's going to affect everyone and it's already a struggle on YouTube as is to be a person that covers video games because so many of the Games already get demonetized But at least you can have the stuff available to watch and if you're a big enough YouTube Or you can get sponsors to come in that it helped offset the fact that you lost all your money on that video from YouTube ads and It's just a crazy situation to me that if the thing is I like Firewatch I think it's a really really great game but if they take out PewDiePie's videos and PewDiePie chooses to fight it and PewDiePie somehow loses it sets a bad precedent for anyone covering video games on YouTube including us Well, I don't think Nintendo is going to go as far as the issue DMCA's that doesn't mean some of the indie games I show all my stuff. I you know, I just have footage of snipper clips Which again Nintendo published that but let's go with one that didn't double dragon for I just had footage of that in a Recent one if they didn't like what I had to say about their game If I decided to talk about it if they they didn't like how I presented their game And I know there's some people there saying oh, you're you were terrible snipper go through a terrible sonic mania We don't like how you play the game. So we don't like how you present the game They could come after me and just literally take out my videos and on YouTube What sucks about DMCA takedowns is if you get like enough of those DMCA takedowns Like it's like it's like two or three of them for different types of videos Like if they do a blanket DMCA take on takes out five videos that just counts as one strike But if you get like that multiple times over different videos those DMCA takedowns literally can make YouTube lock and Delete your YouTube channel So that's why this is a ultra serious thing to talk about Thankfully, I you know based on what the lawyer is saying PewDiePie has some good ground to stand out if he does fight it if he does go to court I don't know if he's going to go to court. I don't know if he should fight it I don't even know if he makes money off those videos anymore Or if you really made money off him at all because you know with YouTube's new policies out there He probably swore in those videos and they probably got demonetized anyways But it'll be interesting. He obviously has the clout and the financial backing to fight it if he would like to You know, I know H2H reproduction is just one a big lawsuit as well. That's of benefit to youtubers I would like to see as much as I'm I really hate what PewDiePie did the disgusting Nature in which he just dropped an M bomb in the middle of a live stream And even some of his other antics. I've really really I'm just totally against I Hope he does fight it because if he does and if he wins, that's just going to help us YouTubers as a community come together For more rights and how we make content because we need those rights man I mean, I'm waiting for for someone to take Nintendo To court and argue about fair use with their games So then we don't have to necessarily go through their creators program. But anyways folks Let me know what you think about this in the comments below again. I'm not here defending PewDiePie I I think what he did was disgusting But I do think that this DMCA takedown is a completely social justice related thing It's not actually related to the streams the streams are allowed to you for 18 months And I believe they forfeited their rights to DMCA takedown and even demonetized videos Based on their own FAQ on their own official website. So, uh, yeah, I don't know That's just my two cents. You guys let me know what you think Whoops In the comments below. I am the Thunder of Agents from Nintendo Prime If you like this video, you know what to do. And if you dislike the video, it's okay. Hit that dislike button Subscribe for more and as always folks, I will catch you in the next one