Yeah, good afternoon everybody The almost I have to say thank you for organizing this really nice symposium necessary for reproducibility of data and It's a really good platform to get to give also a good view of SLI repository Where I work since two decades already And but it is my my last year so I go in retirement led next year. It's a nice Opportunity to meet all you nice people from my clock and from the standard Developing organization and so a real pleasure for me Okay, and fascinating We did not talk to each other But it is perfect fitting of our talks together because I have a really a minor problem obviously minor problem to tell you today But it's the consequences are really severe and I will show in a few minutes, so Yeah, finally cancer cell lines Very nice introduction to cell lines and the cross-contamination situation cell lines have been so this is really a beautiful tool to work on cancer research because because they have been the backbone on cancer research for for for decades and So finally I give also a very very short introduction into the situation So this is a picture of mindful for these nice work of Nicole Surin and What you see is We can We need reproducible scientific data because it is shown you waste too much money It's nearly one billion US dollar a year. It's wasted on wrong cell lines Seline models and even these papers going out of this scenarios and they are missing and this is What we have to to act on and what you see here is Where this patient you get the tumor side biopsy You can establish a cell line from as long in this green area You use authenticated cell lines or even sub clones of this main Population you will get the valid data So the opposite is nicely explained by Amanda the cross-contamination situation invading of a foreign cell into the establishment situation, so this is cross-contamination at source and If there is a little bit proliferation Advantage of the foreign cells, so it will be overgrown. It's just a question of time So finally interchange and also mislabeling also contribute To this situation, but what is really connected and this is also endangerment for reproducible scientific data is this section I want to focus now on and the end end endangerment is done because or by chromosomal instability I will give an example of this and also by micro satellite instability So these are the main drivers of the genetic drift A lot of science scientists call this genetic drift evolution in vitro So but this is not no real evolution because it's not connected to any natural selection Processes this is really genetic drifting in vitro conditions okay, and Yeah, finally, I will go further now to to explain What loss of heterozoagosity in the first line is meaning to a cell because when we do str. Typing's When we are lucky we get the reference profile from a completely heterozygous cell and So carrying maternal and paternal chromosomes. There is a nice de pluit or heterozygous str profile and Yes, somehow there are different loads of Causing events of Copy loss loss of heterozoagosity or copy neutral. This is just meaning that the whole Maternal or paternal chromosome is lost in it to avoid the my top by to Metotic catastrophe The resting one is only doubled, but this is finally going to to convert the double sickness into a single str Profile and I will not go into deep today in In the micro satellite instability situation because micro satellite instability is caused by the deficiency of the mmr system DNA repair system and Which is normally wiping out all our replication errors during DNA replication and This is this drifting can cause Third and or multi alleles at what and this is not meaning that this is occurring in a single cell even if cancer cells are tree or tetraploid and These are sight lines all the sight lines Can carry 10 and 11 or 10 12 or 11 and 12? So these are the situations, but this is not present in a single cell We have done a lot of single cell Authentications and we know that you normally get only a deployed situation Or profile from from these cells okay, and this is always connected to the events or to the phenomena of Loss of heterozoagosity as well as micro satellite instability because there is Yeah, silent danger by wrong cultivation Techniques and this is an image of Karzai et al. So he's working at the right-hand cell bank and I Forgot the citation sorry for that and so he Experienced that if he is growing a culture to complete density Proliferation is stopped and has to start again in the next Passaging so and if you have done this too often if you have an overpassage cell line Then you will work out. So the the sight lines within the population which which are always present They get a chance to fit better the new conditions and then you have the outgrowth of a specific Single clone or a sideline of of cell and and that means if you have an outgrowth you have lost Lot of properties from the whole cell population and this is a problem and Now I will go to in an example because memories are nice examples lasting longer and So there's a pro from from the lab of Perot There's a report that the cell line thp1 Which is an acute myeloid leukemia cell line And it's characterized by chromosomal translocation and it is fusion and two different parts of a transcription factor together and this is and they is doing in Reprogramming in vivo and this is the cause for leukemia in And finally so this is one of the most important cell lines for studying Yeah, leukemia and which genes are affected by the transfusion gene and So they worked Noronia Nandita no, no, they obtained Thp1 from two major bioprositories the SDR profiles who were really very very close together and But finally they found out that the discrepancies they worked out Mainly were due to the heterozygosity and finally They argued that one of the thp1 cell lines is not any more reliable modi for studying leukemia And this although Authentication has taken place so this is a real problem and now I will Try to convince you what they have done so they did from The thp1 cell line from repository a and b they did whole genome sequencing and Finally, they could work out by a new tool, which is electronic SNP choreotyping They worked out for example that chromosome six at least in part is completely Homozygous which is indicated in in a single blue color and while The cell line from repository B is heterozygous for this area and now that you are really drastic Situation is found on chromosome 13. So you see that from repository a The whole chromosome or the long arm of chromosome 13 at least is homozygous while from Repository B this part of the chromosome 13 is completely heterozygous and that means so Authentication has taken place. They are from major cell repositories all claim These cell lines are authentic and useful for our research and finally so I skipped just this in this image and When they worked out with the transchrome transcriptome NGS data they can they had Generated a heat map of this of AML related genes. So that is mean the transfusion or the fuse transcription factor is affecting a lot of other genes and look at this so The row on the right side from the repository B. These are all the target genes which are Upregulated by that by the chromosomal translocation and this is not found anymore in the cell line in THP one from Repository A Okay, and this is Yeah Finally a little bit disturbing the picture Amanda and Jamie has drawn this morning So it's not always black and white and this is completely disturbing a little bit But there are ways out because these findings has also initiated to find and to build up new tools at our repository and first of all The first step should be have a look into Salazar's because and I most respect to your work Tracing all this str profiles to single papers and this is an amazing work. I have really Much respect to do himself and here is already shown that there are the chromosome 13 and 13 here and comes on six here and That there are the differences. So the loss of heterozygosity is indicated and at a lot Yeah, a lot of other salary repositories and the sad story in this case only is That only three repositories are dealing with the right Yeah type of THP one While 12 others are not and that means that 90 percent of I guess So this is an estimation that 90 percent of all THP one cell lines out from major Repositories are not suitable to do leukemia research with regard to acute myelot leukemia Okay, and to to give further. Yeah, this is our own Study, so if you use different algorithms to work out these similarity matches between different cell lines and You work out that only tenable algorithm is giving the difference and not the this one of John Masters But okay anyway There is a we thought we have to to come in into action and what we did is finally we created Yeah, I have to also mention Laura Stainpass. She is new head of our departments in three years and One of the first things we have done then is was creating cell dive and cell dive means that we have meanwhile 160 cell lines are Yeah read out for the transcriptome by NGS techniques and So we have even of course we have our own SDR profile search, which is also public So anybody can use it and you see I just have listed here all the different THP one Cell lines including so in red these are the LOH Problems in in cell lines if they are there and For a much better control because we have the differences these are old cell lines They have been established in the 90s and genetic drift is taking place. So this is biology finally and so We we put on the transcriptome data on our hub and what you can do now I bring you really Quickly through you can give in for example, you can choose and in tighter T So so far we have have been focused on leukemia and lymphoma cell lines, but we have also Meanwhile breast cancer cell line panel sequenced and this is will be updated in this year and so so finally you can Give in the genes you are interested and you can follow up in all cell lines distributed exclusively by DSMZ you see here THP one is Completely positive for one of the leukemia genes DNMT-3B, MyS3, HoxA7 and so on so this is helping The customers that because they can't look Into the physiology of the cell and can check out If this is the right system or not and if the genes expressed I'm interested Okay, finally, I have to speed up a little bit There yeah, cell lines have been the backbone of science or cancer research of the last century this century Because we have much better knowledge about how a solid tumor for example is looking like we have so much Bicep in the cells we have a scaffold and the vessels and in all the this stuff and this This These cells that are contributing because there's cross-talking between the tumor cells in blue this is a hypoxic center, which is dying off normally and loads of immune cells and these cancer associated fibroblasts of calf cells Okay, and I was really impressed by Work from the Netherlands Kerstedal they published in cell genomics and this is really an amazing work because What they did is they isolated primary Colorectal cells Using CRISPR-Cas9 technology to set up the most found mutations in these cells So this is complete knockout of P53 and Specific cells and they so you see the different mutations they put Single or twice or triple into the cells and and they are marked by by colors here And they put also this is a retroviral Induced barcode for identification of the cells so and then they let these cells grow and They formed nice organoids and then finally they did whole genome sequencing in multiple time points during evolution evolution is meaning to grow from single cells to this Organoids and Finally, they what they did then is using the VGS data They have been able to work out copy number variation off of these all the single cells a huge work and single single nucleotide variants Identification so real mutations in the genome Frameshift stops and and so on and finally tracing and All these developments in one dendrogram and this is looking like this and the fascinating point is coming now because these markers chromosome for for colorectal colorectal cancer loss of chromosome 18 and also later on chromosome Part of course home for both of these are lost then they give rise to the most aggressive Colorectal cancer you can get so really aggressive CRC's and So this was a really convincing work They they did because they really mimic Tumor evolution in vitro. So this is a very new step and this is also Yeah motivating us at the SMZ to also look for a new Can some models, but first of all I have to to mention the main players so finally Jesse Burnham and also taught go up from from the Broad Institute and find financial is supported with very well. So this is 2015 and finally and they formed a community of Yeah, high-level labs. I should say and they Worked in the human cancer model model initiative and And these models are explicitly Distributed by ATC by no other by resources But so finally William Sellers CEO of Novartis before and he is now the scientific director of the Cancer cell line factory within the Broad Institute in the right hand of him Monit Singh They have the aim to converse any tumour type into a cell lines for read or Organized model and they are doing this by Genomic profiling of donated cancer cancer tissue and this not after 50 passages This is done after passage two three or four and so they do then screening a dozen dozens of cell culture condition combinations huge work and So finally to to grow the cells and in case of successful cultivation, they do a whole transcriptome sequencing again and Then they do a comparison of the original tumour with the clinical data of the patient and They go then for the next neighbor and this is enabling a very very close model to the original tumour and finally There's a selection of the best cultural condition based on tumour Genomics Finally, they are really successful. I never expected that they as Work out so a tremendous amount of Cell lines from rare and really difficult Tumors and they so over 700 models they got so far and Look at this 47 so nearly half of all these 700 models are of 3d and organoid Morphology and so this is showing us that this will be part of the future also at DSM Z so we have a nice cooperation and I will show that we have due to this collaboration last year. We have been able to complete accession five new cell lines and you see this is New for eight. Yes, it's a long name, but we clearly want to Indicate this is CCLF models so one of the new ones and Yeah, you see this is growing at its face or little organoids are always present starting from from finally single cells but as a biorepository we are dealing with Cell lines which should be infinite because otherwise it's so much work to get them accessioned into cell bank It's really expensive to do so and finally if they are on a primary state They will die off after reaching a flick limitation and therefore I have seen the need to establish a New version of the so-called trap protocol. This is measuring telomerase activity in in in in cells in or any mass vertebrate cells I have to say and Because there is a problem the primary cells There is an end replication problem at the telomeres and every in every cell division They lose this single strand part and this up to 70 80 90 divisions is needed and then the telomere is Gone for example, and then the whole chromosome gets to be in stable and this is Leading the cells into a crisis and then they finally die off okay, and so and we would discuss this with CCLF already and they promised out that they have been really Very successful in to induce telomerase activity by the culture conditions. I Have to be honest. I did not believe this in front of the project and so I finally with my beautiful technician sick family and We adopted an original protocol from the 90s into a fluorescent version of the trap essay by doing a lot of new things and We have been able to create a very very sensitive Essay, which is this is the analyzes in the capillary capillary electrophoresis and you can see nicely every the telomere length so this is the telomere repeats GGG ATT and We can finally work out How active is cell line or the telomeres activity isn't Within a cell line, okay, and Finally So these are controls positive control negative control. This is a cell line, which is there's another way to to prolong The telomeres by homologous homologous Recombination, but this is control to another cell line, which is not and this is heat and inactivation And you see from two of the five CCLF cell lines, which are all positive for telomerase activity That with that they differ a little bit in the condition, but we get the nicest free size only using nine thousand cells in this essay which will be Published during this year in biotechnics that we can go down to to almost 50 cells or maybe less and To get clear signals of telomerase activity Finally, I come to my to to the end So we are happy that we have the first new cancer cell line models in our collection and Yeah, this is a short summary at the end So we need new qualities for increased reliability of scientific data I have shown overgrown overpassaged old cell lines. They can have a problem and and So finally SCR 70 as a reference technique is indispensable There's an endangerment by ex vivo in vitro LOH and MSI There is a need for evaluation I have to convince almost a little bit to work together on this topic to get the most Reliable as they have reference profiles we which should show maximum of heterozygosity and I See the need of e-snip carry-out typing for very exclusive models to follow up copy number variations and This address also to dear young all the new models are free of FPS and They have an improved biological Relevance because of the the the neighborhood they're very close together and so basic research on on tumour cell development should be better and Also drug development should be meet better targets doing this with a new new tumour models. So finally We are going to at the SMZ to going to extension Our cell dive data to to further tumour entities Next will be colorectal cancer Then we have pros promising media matey mediated reprogramming and organoid differentiation technology Implemented by by Laura stand pass and Expanding we are going to expand the CCLF collection of difficult and rare tumour models Okay, this is my former team my my PhDs. So at the end of my Yeah, working time My my technicians and I have to mention of course Laura stand pass with a lot of new input in our department Sonja Abert Abert Responsible for the CCLF collection and Claudia Pomarenke And she is our be bio bioinformatic expert and caught up off which is Yeah, doing all the risk assessments we need for for new cell lines to look for viruses other infections Okay, thank you so much for listening and Let's see Hi, I'm a bit of an off-the-wall question Where do you see automation in these new sorts of models use use of automation to actually Standardize and get the reproducibility going Yeah, not different not not easy to answer finally I see Automatically on all high put through techniques, which means sequencing and all this stuff so there's but Finally, they're still Good manual work Affordable and necessary for from from our technicians because Cultivation protocols even for these a little bit difficult to culture. This is indicated in our catalog that the CCLF models are not easy but we give clear guidelines how to handle them and This will be work. You never I'm pretty sure this can can never be automated Thank you for your presentation. I'm very grateful. You mentioned one word Karyotyping I'm a geneticist by training so I Have the feeling very few people do that still today though It's one of the oldest methods in cell culture to realize what's going on. You know, I remember a paper for 1948 or something Telling us whoever uses immortalized cells that Cells are changing and you can see it on the career type Now we have these modern methods with next-generation sequencing and other people think oh that gives is a solution But I don't agree They what you get in a NGS is an average You get the dominant whatever in your population is heterogeneic. You get a sequence.  He was a really nice person from Scotland and he did all these Karyotyping in our lab in at a very high level including the sky Staining of this different karyotypes Finally the end NGS technology has developed meanwhile so far that we can really nicely and more precisely than ever cover the reads to the density of Yeah, estimate template which is and this is the basics for e-karyotyping or snip karyotyping for the estimation of copy number Variations in in cell lines. So this is really and finally There is no other way because all the cytogenesis are gone So there's nobody who is Really has a good education in this technology anymore I'm sad about this. So I'm a big fan of karyotyping. Yes


Hey everyone welcome to figure fantasy we are here now with leap between scenes and we'll start with easy lineup okay so we have here our lineup so it's important that bring a healer at least and Lafayette will be the middle to be able to help with damage but this is going to be pretty much be an easy stage for you guys and we're done okay guys so see you in the next one okay the next would be hard stage and see my lineup so for this one you'll be needing all five figures and this is roughly your formation you could actually swap Zayun with this guy one in the middle forgot his name Sanada sorry we could swap Zayun and Sanada you could also do that and more or less you will have same result with no problems okay there you go for three star clears off to the next and for our last stage we have torment and let's see my lineup okay guys so this is the last lineup this is a bit tricky you just have to do a bit of a repeat in some of your tries here because you might not be able to get both conditions in the first try especially for the last one for island figure survive the deal don't most damage with Lafayette is pretty much very not very easy but attainable so the last one the four out of figure survive sometimes your enemies get the best of you so that's it there you go so again if you can't do it in the first try just two more tries for the stage thank you guys for staying as far take care stay safe this is the warden and I'm out of here


Hello and welcome. So glad to have you here. As I take a deep breath and introduce for our speaker today, our guest is Sunny White, co-founder and executive director of Mindfulness First. She's here for today's episode of The Nonprofit Show to talk to you, to talk to me, to talk to all of us about prioritizing mental health at nonprofits. And you have such a critical mission and I'm excited. I'm super excited for us to jump into that conversation. Julia Patrick is taking a mindful moment off today, which we are able to grace each other with that time. So she serves as the CEO, American Nonprofit Academy. I'm so very honored to sit alongside with Julia and serve day in, day out as the nonprofit nerd, CEO of the Raven Group as the co-host. We also wanna give a shout out of gratitude to our friends that allow us these opportunities like the one we're gonna have here today with Sunny. But I wanna give a shout out to our friends at Bloomerang, American Nonprofit Academy, your part-time controller, nonprofit thought leader, fundraising academy at National University, staffing boutique as well as nonprofit nerd. These companies are here to pour into you, to pour into your mission to help you do more good around your community. So do us a favor, do them a favor and check them out. And again, without them, we wouldn't be able to produce so many episodes that we've produced since March of 2020. And you can find all of them on Roku, YouTube, Vimeo, Fire TV and for those of you that are podcast listeners, go ahead and queue us up there too, wherever you stream. Your podcast is where you can find the nonprofit show. So today I am just elated to introduce and have what, who I consider a dear friend, a previous client, a current client, probably a former client, but Sunny White, co-founder, executive director at Mindfulness First. Welcome, Sunny. Thank you, Jarrett. I'm just, I'm thrilled to be here. I've been looking forward to it for a while. So thank you. I am so excited that you said yes. You know, it had to be the right timing. And speaking of timing, we have a treat for our viewers and listeners. So Sunny, I'm gonna ask if you would kick us off for a mindfulness moment and then we'll jump into, you know, mindfulness first, the organization, your mission, but get us started and like, let's get us centered. Let's do it. Thank you. Okay, so I'm gonna invite everyone to just sit comfortably on your chair, feet symmetrical on the ground. If you'd like to close your eyes, you may. I'm gonna lead you, it'll be very brief. We're gonna lead you through three deep breaths. So if you want to close your eyes, go ahead or just focus ahead and let's breathe in all the way to our belly, breathe in and then out. Relaxing your shoulders, softening your shoulders and breathing in again. All the way to the belly, fill it up.  And one more deep breath. In we go, all the way. And then as you breathe out, just release any tension that you might be feeling in your arms, your hands, your legs. And then we'll open up our eyes or refix our gaze onto the computer. Hello. Hello, Sunny, thank you so much. For those of you that might have joined us during our moment, again, Sunny White joins us today, co-founder, executive director at Mindfulness First. Sunny, again, so glad to have you here, but tell those watching and listening a little bit about Mindfulness First. We have our episode about mindfulness, but a little bit about yourself and what we just experienced. Yeah, what we just experienced was we sent biological messages to our nervous system, to our brain, that everything was safe. And so our body was able to relax and sort of any stress that we brought to the room just now, hopefully we sort of released that and gave ourselves a bit more presence. And at Mindfulness First, we teach trauma-informed, mindfulness-based, social-emotional learning to kids in schools and to adults in businesses, but I want you to imagine starting a lesson with that mindful moment. That's what we do. We help teachers to learn how to give kids tools to help them relax and be in the smart part of their brain and be able to be present in their classes. And we can do the same for adults too. And the reason we started Mindfulness First was because in 2009, I experienced a stress-induced breakdown and that led me to understanding trauma, mindfulness, and social-emotional learning. And I was kind of like, wait a minute, why didn't you tell me this? You guys knew this? This was an existing fact. And I didn't understand it until I was 35. And so I thought, well, maybe we should be teaching this to children. So that's how we began. What a wonderful mission and purpose. Really understanding mindfulness is where we're gonna start. I love hearing a little bit about your story. I'm sorry that that was the impetus for starting it, but love how you're sharing this with so many and serving that mission. So let's talk about really understanding the journey to understanding mindfulness in the nonprofit sector. Can you talk to us about that, Sunny? Yeah, I have to say, when I first was sick and I'll keep it sort of surface trauma-informed here, I won't talk too much about being unwell, but when I was unwell, I know a lot of other people were sort of leaving corporate America feeling sick. This was around 2009, 2010. I wasn't the only one leaving corporate America feeling very unwell from pressure, stress, all those big, we all know, I don't need to tell you, we all know how we feel. And wanting a change of actually physically needing a change for their own health and wellbeing. And so when we opened Mindfulness First, there really wasn't a lot of this work being done. It was, we found a couple of other organizations like ours who were teaching in schools and a couple of university organizations that were teaching mindfulness and mental health wellness as well. But it was really the early stages. And I have to say, people thought we were a bit weird back then. They were like, what are you doing? But there were people who really believed in us. And people like the Boltz School District in South Phoenix, where we've been for seven years and really made a significant impact on that district. And local corporations like the Whiteman family who owned the Caterpillar Company locally, they believed in us from the beginning. They saw it. They knew that people were doing the same thing and they saw it. They knew that people needed good mental health wellness. And I think as we've seen over the last, while we opened in 2013 and over the last nine years, we've definitely seen that people understand this all a little bit better now. And there's a lot more of us and that's good. That's really good. Well, and definitely going through this global pandemic and we refer to it as pandemic's plural because there's a lot going on in our world. And I think also for the nonprofit community, Sunny, and you and I talk about this often, but it's like, how much can we do with so very little? And we do so much, we day in and day out, pour our heart into everything and often deplete ourselves. So I really think understanding mindfulness in the sector is extremely important and how you're providing the services. Now, you talk a little bit about, and I love this image for those of you that might be looking, it's kind of like what I would refer to as a moped, but it looks like the world is just flashing by them. So the lesson of slow down to speed up, how is that a mindfulness practice and how might that show up for us? This is literally one of my favorite phrases to do with mindfulness first, so to do with our work. So I can definitely speak to this and I can tell a little story a few years ago. So my very close friend, Kimberley, who is the co-founder of mindfulness first and I, it was probably maybe 2018, I think it was the summer of 2018 or 2017. We were burnt to a crisp, we've been doing this work and you know, everyone out there knows, you all know, it's hard work working in a nonprofit, especially when you're building it from the ground up. And we reached the beginning of the summer, I think 2018 and we just said, we need to slow down to actually make progress. We were moving too quickly.  We just took a break. We said, that's it, let's take a break. We need to rest and restore. Our bodies need to feel better because we've been pushing, pushing, pushing and there's been a lot of pushback because people didn't quite understand what we were doing yet. And when we came back in the fall that year, we were so replenished and so healthy and so ready to go. And that's when things really started to change for us. And we've held onto that notion of slowing down to speed up and we try to catch ourselves. When we start to snowball again, when we start to do too much, put too much on our plates, we remind ourselves that actually slowing down helps us to do better and do more. You know, that makes me think a back of the day, I used to do half marathons and there's a Jeff Galloway method and it's all about running in intervals and then walking in intervals. And so that slowing down actually helped me to maintain the steadiness of the timing and that's what really just connected with me. And I think when we look at the scarcity mindset for nonprofits and we talk about, well, how much can you do with so very little? It's like, hey, maybe it's time we take a pause and we do replenish and restore ourselves. I think that's so, so very important. And I love that you live this. I feel like in corporate America, sabbaticals were a thing. And I don't know that I hear that very often. No, I hear too much people getting sick. That's what I hear more than anything. And I think people want to change, don't they? And I think embracing the knowledge of mindfulness and mindfulness is really very biologically grounded. It's learning to understand yourself as a human. And if we can look at our nonprofit as its own little nervous system, as its own brain and body that needs to be kept well and regulated as a group, right? If we look at ourselves as a group that needs to regulate together, then we can make these decisions as a group to slow down when we need to and it'll be for the better of everyone, especially the people we serve, right? Especially those we serve. So there's three approaches that you're gonna talk about here. What are these three? I'm gonna ask you to say them. What are these three and how do they show up in mindfulness? Yeah, so these are three important ideas that we have used over the last nine years to help build mindfulness first as an organization. Now, sociocracy is where we have a flat organization. So it's non hierarchical. And what that means is everybody's really, really accountable. So they're accountable to each other. They're accountable for their own work and it really puts people in their own power and it helps people to get to feel ownership over their work and for us, it was a way of modeling mindfulness. It was a way of modeling wellbeing because all of us had brought trauma from working in the corporate world. We all bought that trauma with us and so we needed to make a change. And also we're a predominantly female organization which isn't on purpose, it just is. And we want to be a safe place for women to work. We want every woman's voice to be heard, every person's voice to be heard. So sociocracy helps with that. And then also we have the worker self-directed nonprofit model, so that means that we don't necessarily lean on the board to set the strategy. We are the people who are working on the ground. We are the ones who say, hey, you know what we noticed this needs doing? Well, that needs doing. And we help to set the strategy in a much stronger way than the board does. And that makes things happen so much more efficiently. And again, it models mindfulness. It puts everybody in the place where they want to be. And then being trauma-informed, I think, well, so that's been a huge part of who we are. But I have to say at this moment in time, it's something we should all be doing because we've just come out of a global pandemic. We have collective trauma. And if we look around, if we look at the people around us, the people we work with, we look at our children, we look at what's happening in schools, the new behaviors, the fear, the stress, it's all a result of pandemic trauma. And so we as an organization, we try to work with our own policies and our structures to make sure that we're trauma-informed from the inside out, so that we can provide trauma-informed services. So for those, again, watching and listening, mindfulness first is providing these services to a low-income population. Is that correct? Like in schools? Mm-hmm, it's true. Yes, at the moment we have, so when, as the pandemic started to come to a close, at least it seemed to, the government really realized that we had trauma. We had trauma for kids because kids' brains don't stop developing until we're 25, 30. And so if you can imagine the developing brain that developed during a time when we were isolated, there was scarcity of food. We didn't see our friends, we were frightened for our loved ones, we didn't know what the illness would do. The breathing, a lot of losses. The breathing, yes, so much. And our children's brains developed in that time. That's why we're seeing these staggering statistics about depression, anxiety, and difficulties for kids, and also for adults, by the way, too. Well, you bring up a great point, Sunny, that there's so many statistics when it comes to the support of this work, there's a lot going on globally impacting so many. So these three approaches, it's very enlightening to me and how these lessons are performed. Now where I want us to go next is how do we make this a mainstay, right? How do we continue to prioritize the wellbeing in a nonprofit? But I also heard you say it's really like for ourselves, prioritizing ourselves, which again, this image off to the right, for those of you watching, looking, self-care isn't selfish, which is such a great, yeah, words there are words. So how do we prioritize this for us? Well, I mentioned just a moment ago, the structures and policies, that is a great place to start. So if we can start with, I'm gonna quote Dr. Nadine Burke Harris here. Dr. Nadine Burke Harris is the surgeon general in California. She is probably my favorite mentor to look to for trauma-informed understanding. And she said that this moment in the global pandemic is the perfect time for us to implement trauma-informed structures everywhere. And she says, strike while the iron is hot. And so that's my biggest piece of advice for anyone who wants to have mental health be prioritized within your organization, is start by educating yourself in trauma. And if you're here in Arizona, you can go to the Arizona ACES Coalition. They do offer great, they're a great nonprofit, offering superb trainings in trauma. And then work together to create policies where everybody is cared for first within the organization because it's like the whole oxygen mask first, right? You put it on yourself first before you help anybody else. There'll be nothing left for us to help our community with if we haven't taken care of ourselves. So I recommend looking at your policies, getting some trauma-informed education or trauma responsive as we're calling it these days. Look at how you're caring for the people who work for you. How are we caring for each other first? That is so important. We've talked so much over the last four, coming up on four years here for the nonprofit show, Sunny. So Julie and I, we've heard from so many guests that have talked about the quiet quitting or the silent quitting. We talk about the burnout. We talk about the workforce, not being there. And when you bring this up about making sure everyone in your organization is well, that really hits home, I think, on so many layers. Where do you think we can start? Cause I feel like there's so much to do. And I know we started today's episode in conversation with that mindfulness moment. And I'm just sitting back here thinking, imagine if every single meeting was started that way, right? Like, so where are some tools maybe that you can give us to tap into? There, you know, we use, there is a, we went onto YouTube actually and found someone who was offering mindful minutes that take just 60 seconds. So if no one in your organization knows mindfulness yet, you can click and play on YouTube.   It has some really great mindful moments that you could use. This is the easiest place to start. Oh, that is a good tip. Now I know a little bit about mindfulness first and I'd love for you to talk to us Sunny about your trainers because these trainers come from all walks of life, even like Fortune 500 companies. So when I think of mindfulness and the mission, you know, that you're delivering, you're delivering this mission, you know, to title one schools, low income, vulnerable, you know, populations, but your trainers, many of them have lived very, you know, well to do corporate America lives, which just, it's really interesting to me. So mindfulness finds its way to so many different people. Would you talk about your trainers and the level of expertise? Yes, yeah, that's so interesting that you notice that it is amazing. Our group of people who, we have 25 people who go out and train for us, but we also have members on our board who volunteer to do our training because they're experts too. And what you're saying makes me think of Tony Williams on our board who is from General Motors and very, very, and before that, he was with American Express. He always teaches mindfulness wherever he works. And what a wonderful thing to bring to such enormous organizations that need that kind of help. And yes, we have all kinds of people. We have K-12 teachers, we have social workers, we have yoga instructors, we have all kinds of people from all kinds of walks of life, yeah. It's so important. Tony is exactly the one that I was thinking of because I can only imagine working in such a very large organization like that and still being able to center myself to prioritize well-being, not just for a nonprofit, right, but everyone. I just think this is such a very important topic. What you are doing, Sunny, as co-founder, Executive Director of Mindfulness First is so very important. I love hearing that there's statistics around this, there's science around that, there's so much legislation now happening because like you said, this was a collective trauma for our entire globe. So looking at that, I would be remiss though if I didn't ask you while you're in the hot seat, Sunny, completely out of left field for you, but I know you're ready for this. What is the future hold for Mindfulness First? Thank you for that question. Yeah, I'm actually so excited about the future of Mindfulness First because we've been doing this for nine years and we're not the only ones doing it, thank goodness. There's lots of us out there across America doing this work. As we've done it, done the work, we've partnered with people like the Arizona State School for the Deaf and the Blind and other organizations. And what we've discovered is there are some big gaps in the mental health education field, the mental health wellness field. And so our future is really full of filling those gaps. That's what we want to do next. And we're going to be revamping all of our services so that we can serve all people, so that all people see themselves in our services. And we will be starting with very special programming for the low vision, hard of hearing in the deaf, blind community, which is a very low incidence community. And so I'm immensely proud of our team members who have brought that to fruition. And I look forward to serving everybody with trauma-informed mindfulness and social-emotional learning in the future. Well, so very important, there's 1.8 million registered nonprofits in the US, all of them with very critical missions and Mindfulness First definitely has a critical mission as well. For those of you this might have piqued your interest, I encourage you to check out Mindfulness First, their web address is mindfulnessfirst.org. Sunny White is there, also active on LinkedIn, so many good resources that your website provides and the organization provides. So thank you. And again, for all of you watching and listening, if you were here in the very beginning, we started and opened in a way that we've never done here at the nonprofit show, but having that mindfulness moment, three deep breaths, really getting into the present and kind of shaking off, if you will, kind of like everything else that might've happened before. So Sunny, thank you for being here and for all that you do. Julia is enjoying a mindfulness day, I hope, which is wonderful. And I'm so glad to be here alongside for the journey. Hey, I also wanna say thank you to our presenting sponsors. They allow us to have these conversations, which are so very intentional and needed, the one that we had here with Sunny White. So thank you. And also to our presenting sponsors, shout out of gratitude, to Bloomerang American Nonprofit Academy, your part-time controller, nonprofit thought leader, fundraising academy at National University, staffing boutique and the non-profit nerd. These companies are here elevating these conversations around the globe. And today's conversation, Sunny really hits home for me for many reasons, but especially as we talk about the nonprofit sector, the burnout, the scarcity, the compassion fatigue, right? There's so much going on. So again, thank you. And for those of you watching and listening, I hope that you'll share this with your board and your team. And yeah, we can all learn and dive deeper into our practice. So I think this is a perfect mantra, although we say it every day, it really rings true in home today more than probably ever, but we encourage you, we invite you, we remind you to please stay well, so you can do well. Thank you so much, Sunny. Thank you. Thank you, Jared. This is really fun.