Hi everyone, Joe for jaspyscasebrakes.com coming out to you with 2019-2020 Panini Crown Royale hoops two box random team break number two You get two random teams each The deuces are wild in this break big. Thanks everybody right here for getting in let's W up like sir mix a lot And there's all the teams right here. Let's roll it and randomize it Six and a two eight times for names and teams one one two three four five six Seven and eighth and final time after eight. We got Dennis down to Charles Six and a two eight times for the teams one two three four five six Seven and eighth and final time after eight. Okay, see down to the Memphis Grizzlies All right, so let's actually make this a little bit bigger here Dennis with the Thunder Chad with the Timberwolves Dylan with the Nets Matthew with the Nuggets Tana with the Wizards Patrick with the Raptors Dennis with the Jazz in the Suns Neil with the Sixers David with the Magic Gretchen with the Hornets Dylan with the Pistons Neil with the Clippers Tana with the Golden State Warriors Charles with the Pacers Gretchen with the Trailblazers Tana with the Bulls Dennis Bucks Cavs Spurs Patrick with the Heat Matthew with the Knicks David with the Rockets Christopher with the Hawks and the Palikins Tana you got my Lakers Gretchen with the Kings and the Celtics Chad with the Mavs and Charles with the Memphis Grizzlies All right, so let's get all this on one screen sort by column B by team We're gonna pause the video really quick and see if there's any trades for the trade window when we come back We'll have the break itself stick around All right, welcome back folks. There were no deals done There you have it two box random team two That list remains the same here are our two crown Royale boxes We've got another two box break in the store right now if you want to run this back tonight I do enjoy the crown Royale a lot of fun here I think there's gonna be a lot of upsets in the tournament this yearly. Oh, there's a kaboom card in there Let's slide the two base cards out there on the bottom See what that kabooms all about There's a rookie crown Royale die cut Hachimura and Kaboom Charles Barkley Yeah, he's fired up he's like yeah Kaboom Charles Barkley Sixers edition going to kneel Stab myself my knife There you go pretty nice. We got knights of the round table Still so pelicans colors there, but this goes to the Lakers.  All right Next box remember folks. This is only break to break threes in the store. So we've only just Just scratched the surface of this case So there's there's a lot of ways to go To chase the monsters out of here So keep getting into it. Crown Royale is usually pretty solid the Tadzee Hachimura Durant Lebron James and Clay Thompson To 99 and Lebron James will go to the Lakers Of course, that'll be for Tana. All right and behind Kyle Guy Or here's Kyle Guy.  That'll be for Gretchen and just South of Sacramento going to Golden State. There's Jordan pool Tana with the Warriors Warriors come out to play There you go, and it's Orlando Magix and Nicola Vucevic 9 out of 10 nice low numbered autograph for David Ross and the Orlando Magic Vucevic having a really nice here this year. There you go, and there you have it boys and girls I'm Joe for jaspyscasebreaks.com. Thanks for knocking out Random Team 2 There's more in the store. Check it out on jaspyscasebreaks.com and I'll break that out with you next time. Bye. Bye


going on, everyone. Sam here with late in sports cards, ripping one box of 22 Panini Mosaic Choice Baseball for Jeff S. So good luck here, Jeff. Here you go, buddy. Neil Cruz and Angel Zerpa on your, uh, the variations. Yep, choice variations. We've got a Johan Durant, Silver Rookie. Mosaic is Tim Anderson. We got a redemption. We got a redemption, Jeff. We've got a Peyton Henry, uh, is for the Marlins, not numbered red auto. Congrats on that, rookie. We saved the redemption for last. We've got Whit Merrifield, uh, and Josiah Gray on your exclusives. Come on, baby, let's see something big. See a big name out of this. Rookie Autographs Choice Fusion. Our number 44, Wander Franca. Go, Jeff.  That's awesome. That is so sick. Congrats on that. That is going to be so cool when it comes in, man. Big time hit for everybody. Nice, Wander. Choice Fusion. There it is. Congrats, buddy. That will get that magged up. That'll do it for your break. Congrats again. Jeff, thanks as always for the support, man. Get it right out to you, buddy.


So good morning, this week we are discussing protein ligand and protein-protein interactions. Today we have a fourth lecture. So in the last lecture we discussed various techniques that mostly detected the small molecule ligand in this protein-protein interactions. Some of the technique that we discussed like STDENMR, saturation transfer difference, water loxie looking at the line width and transfer anyway, how these techniques can probe the ligand side and once we find the epitopes we can even create a docked model or complex model where because we know from where the ligand like what are the sites on ligand that interacts with the protein. So those can be used as a restraint to create a complex model. Now let us go into little more detail and today we will be discussing how you can look at the protein side. So the other partner which is a bigger molecule protein how we can look at this and that is what last time we summarized a ligand based NMR methods for screening experiment to determine in a qualitative manner STDNMR. We can map the ligand like a ligand epitope mapping and then we can use even if we do multiple concentration like what we discussed is called STD amplification factor we can do that and we can find it out the dissociation constant in a quantitative manner. Then we also discussed saturation transfer double difference for whole cell binding. So we ended up here. Now today we were going to look at the other side that is protein based method. So we can classify this ligand protein interaction into two ligand based method again it relies on knowledge of property of ligand small molecule typically then we can look at the chemical shift perturbation the relaxation that we already discussed and change in their resonance position and also how they are diffusing so diffusion coefficient upon binding the small molecule will diffuse differently than whenever it is free. So all those we had already finished it so all these are done. Now protein based method basically we are now looking at a bigger molecule protein right. So we need to have a knowledge of protein how what is the structure of this what is the function of this protein. So structural functional aspects of protein should be known when we are using this for a protein based method. Now actually the deterministic signal that comes from protein is chemical shift change change in the chemical shift so we can do SAR by NMR monitoring how chemical shift is changing and typically for doing this 2D heteronuclear single quantum coherence is used 2D heteronuclear single quantum coherence is used right where each peak gives an idea of one of the correlation like if you are putting it 15n and proton correlation so that means each peak which is here is reporting about one amino acid and the changes that happens in these amino acids essentially can be captured when we do protein based NMR methods. Right so what we can look at a protein detected experiments so you need to first stably like a label this with a stable isotope and here our ligand can be unlabeled. So protein has to be labeled because now we are looking at the protein and since protein molecules are big so to get a resolution to understand more in details because now we want to go and go to get residue specific information. So we need to enhance the resolution by isotopically label these proteins however our ligand can be unlabeled. So what we are going to monitor here is a chemical shift. Now chemical shift of a protein actually may change upon binding or intensity may change upon binding so one of these parameter we are going to monitor. So the change in the chemical shift change that the chemical environment of that particular nuclei changing and that basically can report about what is the on rate of binding, what is the off rate of binding, what is the KX exchange chemical exchange phenomena and using that actually one can get the KD of the binding right ligand protein binding. Then we can even find it out the binding site on the structure where from where the chemical shift is changing what are the residue that are engaged in this interactions that is going to tell us about binding site mapping we are going to discuss this in detail. Then the other parameter that we can monitor so if you remember in the last class I just on the basis of one day I showed that here is one parameter called chemical shift another parameter called intensity and third parameter is called say lambda line width these three are easily determined like we can determine these parameters easily. So chemical shift tells about the chemical environment, intensity tells about how many like a what is the population that is being participated in these. So intensity also can tell you about binding site mapping on the structure and using this of course one can get it K on, K off, K exchange and KD. The third parameter is line width right so line width depends upon T2 the transfer relaxation rate and if the molecule becomes bigger and bigger your line width changes and that also tells about this changes so for a unleganted you have a sharp line once it binds you go to a broad line so that generally the it may go depending upon how binding is happening you go to go and get the broad line and that also tells about the binding phenomena. Then we will be also discussing some of these other techniques like a CPMG techniques that essentially is used for determining the lowly populated state in the protein ligand interaction or even protein conformational change and ZZ exchange we are going to discuss both of these. So essentially depending upon what we want to probe so CPMG actually probes the exchange between two states and if they are slowly exchanging then ZZ exchange can be probed. So briefly we are going to discuss these two techniques in coming slide. So let us start from the beginning so as we said if we are looking at the protein we need to have some structure function information of a protein and just to recapitulate whatever we have done recap of the steps involving protein structure determination by NMR so essentially what we are doing we are going from primary sequence to three dimensional structure and what are the steps involving doing this first you have to prepare the sample. Now our sample has to be isotropically labeled so you feed your bacteria with ammonium chloride and carbon 13 glucose and or like you can be singly labelled only ammonium chloride and or ammonium sulphate or for doubly labelled your glucose also has to be 13 C labelled. So once we prepare the sample we can go and collect the data multidimensional data. So multidimensional data will be collected now since this is our said data coming in the background also have a data so we collected data. Next step to identify what is this peak what is this peak what is this peak so all these peaks identification is called resonance assignment so you have to analyze this data. Now we have a name for this these peaks it can be some L, M, N whatever amino acids so and for structure determination you have to generate restraints so distance restraints angular restraints and all those you have to generate which we have already discussed and then you calculate the structure three dimensional structure using these restraints angular restraints the bond vector restraints and the distance restraints. And finally we incorporate all these restraints for a structure calculation we determine the structure and finally we validate this structure what is the quality of the structure. So once we have a structure we are all set to understand how this protein is interacting with another protein or another ligand and that we can now we are going to use this information which we have captured here during the structure determination of the protein. So just to summarize suppose I have a protein which we are working in our lab say SKP1 S phase kinase protein some protein which is involving ubiquitination so it is one of the component in E3 ligates so do not go in technical details so first you have to purify this protein right. So you have to be getting the exact molecular weight so you can do either SDS phase you should have a single band of these proteins then you can validate using Maldi techniques so you are getting the exact molecular weight this is m by 2 so you are you are sure that my protein is absolutely pure and this protein sample that we are preparing is at least n15 level. We also determine the secondary structure using one of the low-resolution structural technique like circular dichroism so one can see here it is showing helical characteristic and you can see here lots of helices are there so now this corroborates that the structure which should be captured is showing helical. Then we recorded the HSQC spectrum using various sets of 3D experiments that you have already done one can assign each of these peaks so you each peaks has a name right so this is say V25 this is K113 this is say A52 so now we have now once you do titration experiment or the binding experiment the any perturbation you see here can be captured and that perturbation is essentially used to understand protein ligand or protein protein interactions that is how we are going to do now. So suppose this my favorite protein SKP1 is binding to its partner called SKP2 and binding site is here so what do we expect right from theoretical knowledge so this blue is SKP1 and this the orange color is SKP2 and binding site here is shown indeed so suppose we are studying this protein right we are doing the titration of experiment. So we assume we are saying that there will be some change in the chemical shift so to start with what we are doing we are taking the labeled SKP1 we are monitoring on SKP1 and then we are taking unlabeled SKP2 we are titrating it and seeing what is changing so suppose change is happening so I have taken here two residue here in this plot and here also two residue if you see one residue is very less changing something is happening that we are going to discuss little more detail so intensity is changing but here you can see it is a chemical shift is changing in this case also this guy is not changing A6 and say some name like A6 it can be from other protein so some amino acid is not changing and here one amino acid is changing very nicely if you look at if we are titrating it adding more unlabeled SKP2 peaks changes so now change in the peak at least is telling that some interaction is happening right some interaction is happening and now that is what we are going to monitor so chemical shift is absorbed for the various species and those are the powerful probe to understand the binding interaction. So we have taken the labeled one part not unlabeled another part not we are titrating it adding and we are maintaining some structure material for each titration we are monitoring what is happening in each peak and a peak wise manner we are going to assign and understand the point of our interaction on the protein side great. So as we saw there are there are certain phenomena happening peak is shifting or peak can appear appear at a different position or it can disappear so now appearance or disappearance of this individual peak that means change in the intensity or line width can vary and that needs to be quantified for finding it out actual binding effect and that depends upon several property right several property of these interactions so let us take it some of the parameter which will help us and understanding the protein-protein-protein ligand interaction so suppose we have a free protein which we define as a P it has concentration of P now or a characteristic P and a free ligand which is L and a complex which is forming PL so for a free protein there is a chemical shift right which is omega P and when you remember what we are looking at only protein ligand is unlabeled so when protein forms a complex which is PL now its chemical shift is changing from omega P to omega PL right so the change in the chemical shift that we are monitoring is delta omega that is happening because of this interactions so we can say omega P minus omega L so to make it more understandable here is my omega P and it has shifted and made it something like omega PL so this difference that we are seeing omega P minus omega PL is delta omega so change in the chemical shift of this protein which is labeled is delta P okay so upon this interaction we are getting this parameter now the appearance of different species of protein in the NMR actually varies it also depends upon what is the concentration we are choosing of various species protein to ligand test stoichiometry it depends upon what is the rate with which they are associating what is the rate which we are they are dissociating so Kd is one of the important and what is the exchange between them so how these two like two protein bound and free form is exchanging between them so K exchange in next slide I am going to or next couple of slide I am going to discuss in little more detail so let us define this K exchange parameter K exchange parameter is K on into ratio of ligand and plus K off so one can determine subpopulation of free protein one can get it K off divided by K on into ligand concentration plus K off and bound conformation can be P like a population of PL K on ligand concentration divided by K on L plus K off so if we know the ligand concentration if we know the rate and population we can find it out what is the free population what is the bound population so by getting these chemical shift and ligand concentration we can also get the population which is bound form which is in free form and various thermodynamic parameter so you can see now we are now going little more detail in a quantitative manner of binding in terms of thermodynamics in terms of kinetics. So as I have mentioned one of the parameter that we were discussing is Kx so chemical exchange this is called so two or more state right during the time of recording of NMR how they are changing that is called exchange phenomena suppose this is my protein cartoon we have made and this protein is changing its conformation right here it is a change in the conformation. So the rate with which the exchange between one conformation to another conformation when we are recording the NMR spectrum that is called chemical exchange. So typically on the NMR time scale this K exchange may vary it can be fast it can be slow or it can be intermediate so depending upon what is the magnitude of Kx. So chemical exchange typically can be slow if the two states are slowly exchanging with each other what is the slow with respect to NMR time scale again I am going to explain you what is the NMR time scale because we are recording the spectrum so when they are exchanging slowly so we can probably get the two peaks one coming from state A say state A and one coming from state B if they are exchanging very slowly the another phenomena can be intermediate time scale it is neither fast nor slow so like you see fan if fan is rotating very slow very very slow you can see all three wings right if it is with some speed they are like they are with some speed they are spinning where your eye cannot resolve it but you see and averaged right average state of these that we can call is intermediate states so where the contribution from A and B are so merged that we are unable to distinguish and then there is a fast exchange with respect to NMR time scale so then you see an average state like if fan is spinning very fast you see an average value of these three wings or average phenomena of these three wings so that is a fast exchange so at the NMR time scale the exchange between the two states can be slow fast or intermediate and that based on the magnitude of Kx right so what is the NMR time scale and that that we are talking and exchange rates so when we are probing and say protein ligand interaction using a standard either one-dimensional proton NMR or 2D NMR spectrum that is what we are going to do so for labeled protein we typically decode this HSQC but you can use also 1D NMR that we had discussed earlier right so what is the lifetime of say bound state and free state how these say bound state or P state and PL state how they are exchanging and so the lifetime of bound state and T free state is tau and then it depends how accurately we can determine the resonance frequency of this P state and PL state respectively so that is given by the lifetime of these states and that is say delta omega so the change in like a difference in the resonance frequency of omega P minus omega PL is our delta omega and that is correlated with this Planck's constant with a lifetime of these two states how what is the lifetime of bound state or free state so delta omega is the difference in the resonance frequency for these two peaks so K exchange that is per second will be given by the lifetime of this one by tau and that will be respect to this delta omega that is in radiant per second so if the lifetime of two states is very short very short then the difference in the resonance frequency cannot be measured right so that is that is they will collapse to a measured signal if the lifetime of these two states is very short so like they are so short they are exchanging very fast so you cannot measure it at the NMR time scale it will be unmeasurable you get a collapsed state but if they are slowly exchanging the lifetime is quite long you can measure it right so that is what happens in slow intermediate and fast exchange so when an exchange rate is much slower than the observed difference in the resonance frequency the exchange rate is is said to be slow on the NMR time scale right on the NMR time scale so here is a protein and here is elegant right so they are slowly exchanging so kx one can have which is 1 per second to 10 per second you can see in slow exchange depending upon what is the kdk of rate and all those we are seeing two peaks clearly two peaks one peak coming from the free protein one peak coming from the protein in complex with elegant two peaks we are getting so our k exchange is much slower than the delta omega change in the difference in the chemical shift of these two and even in 2d you can measure so we are getting clearly two peak one coming from the free protein one coming from the protein complexes which is here okay. So now this is called this slow exchange where kx is much less than delta omega the difference in the chemical shift of these two states. The another one which is called say intermediate time scale regime where our kx exchange will be roughly equal to the change difference in the chemical shift of P and L. So here suppose this is for P and this is for L so you can see now lines are getting broader and exchange rate between this so here line is going down here lines are coming up here also you see the lines are getting broader and lines shifting so depending upon what is the even in the intermediate regime what is the exchange between these two you see peaks is slowly shifting and disappearing that is the intermediate time scale. So as we were giving analogy of fan if it is speed spins so that we cannot differentiate between these two states our eye cannot resolve it that is what is the intermediate state k exchange. So here the difference in the frequency will be equal to kx kx of the of two states. Now coming to the first exchange this is the regime where our k exchange the exchange between the these two states omega p and omega pL is very fast happening very fast happening so k exchange is much faster than delta omega of these two so these two states are closer but peaks gradually shift to a new position and we have a average value that we see so this is fast exchange happening very fast. So these two concepts or these few concepts of slow exchange intermediate exchange and fast exchange are measured in terms of what is the difference in the resonance frequency of these two states at the NMR time scale with respect to NMR time scale and that is basically used in the NMR. So here one can see now in the fast exchange regime our peak is moving from one position to another position great. So now coming back to how we can use this so suppose in fast exchange regime the chemical shift in the either dimension either of two dimensions proton and nitrogen can shift either proton can shift from one position to another position nitrogen can shift to one position to another position so in that case one has to take the change in the chemical shift combination. Now how do you normalize because now proton chemical shift varies from 0 to 10 and this can vary about 30 percent in protein like 100 to 130 so you have a normalization factor that comes from the gyromagnetic ratio you can measure it delta 8 combined will be change in the chemical shift frequency of proton square plus 0.1 which is coming from the ratio of gyromagnetic gyromagnetic ratio 0.1 multiplied with the change in the resonance frequency of nitrogen just square it take under root that is a combination you can see fast exchange since proton and here proton and nitrogen both axes are moving so in those case you can find it out how much chemical shift is changing great. So we find it out or you just show you one example of non-covalent binding of two proteins so here in my lab one of my PhD student titrated N15 level sumo with its binding partner called E2 and he found some of the peaks were shifting little bit some of the peaks were disappearing so he quantified it and looking at these values since peaks were disappearing more so we are knowing that this is happening at the intermediate time scale it is not a fast time scale to substantiate that he did also ITC experiment that I explained you in the previous class this is a thermodynamic parameter experiment that measures the various thermodynamic parameter and one can see here when we titrate sumo with its binding partner called E2 we are getting a typical KD of 2.5 micro molar and looking at this peak disappearance few of the peaks you can see here either shifting or disappearance we are knowing that this is happening at intermediate time scale. So these two techniques are corroborating very well KD at a lower micro molar range and peak disappearance happening in the NMR one can say that the exchange between the bound form and a complex form of N15 level sumo was at intermediate time scale. So he found it out not only the position where it interacts the order of magnitude of the interaction also one can find it out. Now another example I am showing you here is another protein which interacts with one of the metal ion so we titrated with metal ion and what you are seeing here few of the peaks just zoom it here like here we have A119 you can see nicely the peaks are shifting here 120 peaks are shifting here. So these peaks were shifting and they are telling that these are the residue that are involved in the interactions. So you can map those interacting site on this structure now you know where this metal is binding. So not only you got the thermodynamic parameter by fitting it which I am going to explain in the next class how you fit this change in the chemical shift to get an idea of exact binding constant but also we got the location where actually it binds you can map those on the structure and find it out exactly the binding site that is what NMR offers you not only the position of the binding but also it also offers the thermodynamic parameter here qualitatively I told you what will be the order of magnitude next class I am going to explain in a more quantitative manner how we can use these to find it out what is happening. So there are like that I will stop it here and in the next class we are going to discuss to advance technique which is used for understanding the protein-protein interaction is called CPMG and ZZ exchange that captures essentially the intermediate scale exchange like a micro molar exchange and a slow exchange that we are going to discuss it and then looking at some of the quantitative aspects of interactions these interactions how we can determine the parameter. So hope to see you in the next class thank you very much for your attention thank you.


Hey everyone, welcome back to alchemy star. So we have here the marionette Leonore and we're going to be summoning for her in her banner Hopefully we could get her early if we do we are going to try to summon Here in black magma, which is giles or giles. So without further ado Hopefully we can get her in 110 pull Wishful thinking. So anyway, let's do this 110 pull. See what we have here So it's oh, it's It's an orange Shall I get her did I predict the future or not? So we have a yellow here So this is good at least if it's not her then still have more Currency to get her so but I'm really banking on getting her here. It's sharonah It's okay. I'm happy with that. I'm happy with that. I'm happy with Sharuna have her now at breakthrough two or three not sure but happy to get her Who is the yellow? Aurorian that we got Not gonna say that this is a good, you know good session my jinx me later. So Might as well keep my mouth shut Keep my mouth shut who is this this is Barton already have a copy so Barton so There we go Sharona in the bag and let's move on to the next Sharona Sharona So let's do another 10 is there a an orange here in these pools. No No, no, this is a violet Skipping skipping skipping skipping. I'm sure I have them already okay moving on to This is my 30th pool. So this is the 20th actually for the second set because It's yellow it is yellow One yellow Who is this? So I'm counting 20 because we're gonna have to reach the pity again because we got A six-star a while ago. So we're gonna have to Reset the pity to 20 pulls we need to reach 50 in order to increase the percentage Zoya next is quickly quickly Quick quick quick, please be quick about it Tessa Who is this? Hopefully it's somebody that I can Know have copies of Beverly ready. So skip the rest Next next next next Okay, so we're proceeding with Lumamber ready, so let's do this Lumamber Okay, so this is the 30th pool Still yellow hopefully two yellows Two two not only one only only one So hopefully we could get alien or Through this new Noah Okay skipping ready have no at the max breakthrough Okay, this is 40 40 For the 40 it's still yellow Only one yellow After the first pulls seems to be going downhill from there So this is 40 next is 50 then single pulls from then on So don't tell me this is oh Regina. Okay Never mind Regina We have This is 50 50 pulls I think my math is right Hopefully it's right Okay, so this is orange ready. Hopefully this is ready you know So that I'm actually two yellows I'm actually planning to save my Lumamber for The support banner support recruitment banner Hopefully I could get I could you know Get her now and stop and move on because if if I proceed to two to giles I'm actually thinking about it, but this is Lenore You who? Yes To yellow coming in So let's check if we need to to Break through her as soon as possible. Let's check out her breakthroughs This is going to be Roy here. I've copies of Roy ready. So and the next one is Narok sell I Think that's good. I think I need Narok sell So so far So good If we have ten thousand left. No, no, no, I have seven thousand left. So that's two pulls never mind I won't pull I'm sir. I'm reserving that for the support banner, which I want to get No view there Oops sorry Let's check her kit out So that you guys if you haven't pulled for her yet, you will have a preview on how her breakthrough is Going to look like Green green there you go. Okay. So breakthrough for her First is HP Second is defense Third is active skill enhancement each every time an active skill cost damage lennors max HP is boosted by 3% Which is nice additional survivability for her Okay, so Probably get copies of her in the future, but for now, I think I'm good. I'm happy with my pulse I got Sharona and her the rest of these I'm I'm expecting a you know a support banner coming in so I'd like to pull on that because I lack a lot of Florine no view is there Both of them. I really need in my roster. So Again guys, that is it for the recruitment session. I'm I'm actually itching to pull for this guy Ford for for giles, but it's it's it's only going to be 20 pulls so don't want to waste it I Gonna be using it for the targeted recruitment support banner. So technically I have four so I just need to four 40 pulls 40 plus pulls So I need extra more just to get pity and hopefully I could get either no view or a Florine from that matter. So anyway guys, thank you very much for staying this far. So hopefully you have better luck in Your pulls than I did. So stay safe. Take care. This is the warden and I'm out of here


a game you saw on prime video the Yankees come from behind their 29th come from behind victory beat the Royals 11-5 at the stadium and Jack after the Reigns came a the floodgates with the Yankees offense opened right Chris you look at this game and before the Reigns did come the Yankees played one of their worst half innings of the season a fifth inning in which the Royals were able to score five runs couple of defensive miscues Cole ends up giving a couple of big run scoring hits in there including a three run home into Perez but then fast forward to the eighth inning the Yankees have one of their best innings of the season half innings with a little help from Kansas City because an error helped fill the Yankees and of course the biggest hit in that inning because he's had the biggest hits for the Yankees all season Aaron judge has a grand slam homer it was his second home run of the night and there really shouldn't be any debate any longer about who the American League MVP is he was playing at Yankee Stadium tonight and he was continuing to do otherworldly things


thank you so much guys for staying with us it's been so much fun i mean like a hundred challenges and you chose to stay to stay with us we appreciate you so much i mean uh let me shout out a few people just because we're wrapping up because of time i have jammy moira uh he's like rock two or two five four loving the show loving your show you guys watching from magana town kiambu shout out to kiambu county yeah i'm really enjoying the show so much thumbs up that's major and then i have um i have elvis by the way he says are my favorite oh no i have that is daniel cobb 46 he says are play monsters and men please buy power slide yeah and i have someone saying in my favorite band i'll never stop bleeding power slide yeah shout out to power slide i'm telling you yeah that's cool i super cool i i know i know like it's a rock thing so it's cool it's cool and then i have edga our room rooty and i say my room rooty too and i shout out to edga now come here edgan will i say he's always tuned i'll be shout out to you i'll have john matthews he's saying flare raining blood all right i see i see i have pole moho in the rango and i say we're watching from jambini play for me my limoengu by samuakwiri all right okay i'm bannard ndumu and i say my watching show niki wavashio kabati well shout out to everybody who's tuned and we appreciate you guys so so so much this is to s and gressy who are chilling out with the ladies thank you for showing up yep all right i have i have someone regarding our topic of discussion today we're talking about dealing with stalkers i have a voice of mine elvis greth and i say my um rock tour at what for channel rock tour ignore is how to deal with a stalker don't give them an audience because if you do you know just like i said ignore ignore ignore don't don't give them any attention and this goes to all the girls by the way you have to be responsible and men too don't take money just from anyone don't take money you always have to pay everything has a price by the way so don't take if you're not ready to do something for someone don't take their money or just don't take the privileges they're giving you if you're not ready to give something in return before you take someone's whatever they're offering ask them what they want in return because people always want something so ask them you need to attack our ones out before i take your money don't just take money from people for no reason people work hard for their money i saw someone saying that and i actually agree with that a hundred percent people work hard for their money so someone will not just give you their money for you to play with them if you're not ready to be someone don't tolerate them like if you're not feeling someone don't waste their time just be like oh i'm not feeling you so don't you know just don't avoid first yeah right so yeah something happened just yesterday um i don't know whether this is suitable for the program but um i'm going to see my friend she had this shirt this very nice shirt i had to wear for the people on tv yeah so i went to get it back and um as i'm making my way back home uh some guy on in our car stops then he goes oh i'm free food then i i think that he's maybe lost way he's looking for directions or something so i just wanted to be nice and then um i go i go to the to the window then he greets me then he's like um then i tell him i'm going to see my friend and then he goes like i saw him in gear 20 no don't get to strangers cars if you don't know someone don't get into their cars no no no no that is no people creepy things are happening by the way people are people are being kidnapped other people are getting killed like it's not funny so don't do that but shout out to everybody who is tuned by the way we appreciate each and every one of you if you didn't get to play your track or just to shout you out we'll do that next week we're really strange for time so uh yeah but where are you playing where can people find you where are you playing yeah today today i'm not playing today i'm going to go today i'm getting digs that's cool so shout out the show runs every single saturday from five p.m all the way to seven p.m we appreciate you the rerun is on tuesday at 8 30 p.m if you happen to miss that and on wednesday we're playing you all the you know marshy music that's on wednesday yeah we have yeah edm you know all that cool music that is uh five p.m on wednesday and on sunday tomorrow we have uh gospel rock so tune in from five p.m now we have to go we'll be leaving you with first like song or banana republic do enjoy it and we'll see you next week bye


Hello and welcome to Alabama Extension Sustainable Agriculture Research and Education Program at Auburn University. My name is Ayanava Majumdar, doctorate from Auburn University. I'm extension entomologist and also coordinate the beginning farmer program here at Auburn University. Today I'm going to talk very quickly about the three levels of pest management that forms the basis of the sustainable production system. So let's start out by talking about the first step to IPM or Integrate Pest Management which is insect monitoring. You need to know what insect you have whether it's a beneficial of a pest before you make any decision to manage them. So the thing that we use in the IPM program very effectively are sticky wing traps and here is a one of the sticky wing trap bottoms that shows the moths and this in this case this is a squash wine border which is a very difficult moth to track but you can capture them easily in these traps and know the numbers if you see this many moths in the trap you know there's trouble coming. I also keep some other things handy such as a yard stick so I know how many if I'm looking for caterpillars I know how many I'm counting in a certain distance and then I also carry a scouting flag. These tall ones are very good at marking a spot for example if you found a aphids at a spot put this down so you know you can come back in two three days and count again to see if the aphid numbers have gone up so these are some of the basic tools and don't forget to have a good magnifying magnifying glass with you so that you know if you're working with small insects also use your cell phone you have a camera on your phone use that and take pictures and send it to extension. So if you have determined what insects you have and if you have say for example leaf-footed bugs in tomatoes one of the first step you can do as a sustainable IPM practice is trap cropping and here I have some examples of trap crops this is a NK 300 sorghum head which is extremely attractive to leaf-footed bugs, sting bugs and then this is the peridovic type sunflower there's also other varieties of sunflower that are very attractive leaf-footed bugs and the trick is to let the insects come to these trap crops and they spare your main crop which is tomato that you want to sell or eat. Here's another example of a trap crop which is New England Hubbard this is a Hubbard squash that is extremely attractive to cucumber beetles squash bugs which are our major insect pests in the squash production system and this is an example of a trap crop but you can actually harvest or sell the trap crop and it's very easy to get these through online with seeds from the seed companies so this is your first level of pest management the next level is pest exclusion system and there's a lot of research going on in pest exclusion system thanks to our various funding agencies but just to show some examples there's two types of pest exclusion system the temporary exclusion system and the permanent system let's talk about pest exclusion system especially the temporary pest exclusion system which is very suitable for home garden and small farms so here I have different models of pest exclusion material you can buy online these days it's really convenient and it's worth the shot behind me this is a kind of a greenhouse type mini tunnel that you can see that has clear plastic on the sides and it has plot on the front or the end walls if you want to call it and this actually comes with a neat zipper where you can open the structure for ventilation so the zipper really helps to cool down your plants when it's too hot you can also access this your plants say for example you have to weed weed control you can open it you can hand weed or you can do a pressure application in here as needed but it is pretty warm in here whatever you're using these structures don't forget to close them up because they keep insects away and that's the big benefit you have on top of the rain and the wind protection you're getting make sure you also have your irrigation system set up before you do something this elaborate let's look at this next system which is basically a fleece grow tunnel and it has two parts the fabric and then the hoops you can buy these as kits online or you can buy the fabric separately so in my hand I have the super light insect value and you can get these hoops you can make them yourself or buy them online and you put them up over the over your crop and you can lay the fabric on the top try not to put the fabric directly over the crop because insects will get through the crop and they can still sting the crop so to keep the insects away from the crop make sure the hoops are raised up and then seal the seal the front and the back so that insects don't get in let's look at the last structure we have which is a plastic low tunnel or caterpillar tunnel whatever you want to call it and this one has again the hoops you can buy this as a kit and it has this plastic shearing which keeps the insects out it also raises the temperature inside it's similar to the other greenhouse structure we saw and you get a lot of benefit almost 10 to 15 degrees difference in temperature so this is really good for your early season start early spring when the temperatures are fluctuating and these all this fabric they will help to keep the temperature warmer and let the plants grow faster you also have to watch the amount of irrigation you need so you don't irrigate because this will exclude plastic will exclude rain so you have to make sure you you are putting the irrigation system and watch for weeds that pop up but these are very useful for growing crops and for your IPM solutions if you are a high tunnel producer you may want to in to have a permanent system of exclusion and for that you can use shade cloths like these black shade cloths or different colors to keep the insects out physically from the crop so pest exclusion system is really an amazing way of doing it but you have to learn to do it don't forget the beneficial insects there are a number of beneficial insect that are sold by the companies they're in your field make sure you're protecting the natural enemies by reducing your sprays and and you can buy these online very effectively from different vendors so in my hand is one of my favorite which is the lace wing but you can buy lady beetles assassin bugs lot of different choices if you are done with these IPM systems the next is use of bio insecticides or whatever insecticides you prefer but be please be careful with using any insecticide do not overuse resistance is an issue and you don't want other insects to flare up so this is really insecticide is really your last choice and if you're organic certified organic check the Omri label Omri stands for organic materials research institute that label must be there on the product and label is the law so please read the label the fine print on the back to see which crops it's effective for with all this information is available in our slide charts and I have some examples here here's an organic IPM slide chart which actually lays out the three levels of pest management we just talked about for example the sustainable system the first practice level one level two which is the pest exclusion systems and level three which is use of bio insecticides so all of this is laid out in the in the organic slide chart which we update periodically and this is available free of charge to all producers in Alabama and everywhere this is the urban farm IPM toolkit also suitable for home gardeners basically there's a home garden insect control guide and this tool condenses that guide into a really nice handy system with pictures that you can use we also have different publications like brochures for the beginning farmers we have high tunnel handbook crop production handbook for beginning farmers and then don't forget the vegetable handbook the southeast vegetable handbook remember to check the year and get a fresh copy every year from your regional extension agent or the county extension office and finally don't forget the farming basics phone app don't forget the farming basics phone app that farming basics phone app has your communication tool so you can call the agent find your agent call the agent it also has insect and pest database in here and it also allows you to contact us through the feedback so make sure you're using the farming basics phone app and for any other information please feel free to call me my phone number is<PHONE_NUMBER> and my email is bugdoctoratoburn.edu thank you very much for your watching this video


Okay, welcome guys to quantum healing experience I'm David Wong and The I'm the Chi master I'm the founder of company called Chi life and We focus on harnessing Chi energy What is Chi energy? Chi energy is life force. So it's within us It surrounds us and it permits the entire universe just like Star Wars And it's real so Over the last few years. I've been developing technology that Harnesses this energy so that you and everybody in the world can benefit from it and can Transform themselves with it because Chi energy is For four thousands of years have been throughout many different cultures and many Cultures have written about it they've studied it and have used it for many things such as for healing for Manifesting for spiritual practices and even for superhuman abilities like for fighting like martial arts for telling the future for telepathy for clairvoyance for astral travel all sorts of different things have been documented and Recently science has discovered that it is actually real and you can actually measure Chi energy You can measure the effects it has on human beings. You can measure effect. It has on actually inanimate objects as well like even it can use Chi to affect electronics to affect computers to affect software and to affect What is not even? What people would think has no consciousness Chi energy can actually affect that as well Pretty interesting, right? You guys. How you guys saw seeing the movie the matrix You guys welcome. Hey lady homestead. Hey Raphael. Hey, hey Tony. Hey, Joseph and Hey CC CC hey Gloria welcome You wish it this worked you wish that what worked Chi Chi energy works Well, you don't need to wish because it already works and You can actually try it out and see if you get sensitive to this Chi energy I created a program that is completely free that you can try out to see if you are Sensitive to the force the sensitive to Chi energy and we found that about 70 to 80 percent of them of people who try this they can feel it right away and The other 20 percent they won't feel it right away and the reason is because just some people are more sensitive to vibration to energy and to To Frequencies in general, you know, some people just have no sense of that It's the same where some people have no sense of music people have no sense of taste and in In design or or art, right? It's something that some people have and some people don't have so what we what I did is I created a System that you can test yourself with to see if you are Sensitive to the force to see if you are Jedi material Okay, so who wants to know if you are Jedi material? CC says it absolutely works. I promise I have been playing the energy signatures in my home for six weeks now Yeah, so you see he she She tried my Chi energy AI. That's what I'm talking about. So Chi energy AI is the technology that I've developed combines quantum entanglement and AI, okay? So it's basically it's a software and has its own intelligence that can basically send Chi energy Through your phone. So your phone becomes connected with this energy source and then now your phone can Broadcast or emit this energy source graph. I also says I've seen the matrix you want to know, okay? So if you've seen the matrix There's this scene that really boggled my mind what the first time I saw it when Neo was rescued and He was in the spaceship or whatever he called the the ship and Then the sentinels, you know those robots were after him and he was blind at that time And then he stepped out the ship and then those sentinels were coming towards him and they were completely defenseless their ship was Had an EMP blast. So it was not working.  How could he actually affect? These robots outside of the matrix and then later on I the idea was that Everything was connected the real world and also the matrix is connected together So Neo had power inside the matrix you can do Kung-Fu you can do all sorts of stuff You can fly but also but when he came back to the real world He also had power because he was that the matrix was connected to the real world. So that's what I'm saying, okay So that concept bring that concept into what I'm doing here You think that Technology is disconnected from humans, right? we think that the matrix or the internet or Cell phones are disconnected from humans and our consciousness, but The reality it is directly connected to our consciousness That's why our consciousness can actually affect the results of computer programs and our consciousness can affect the The results of randomized number generators This has been proven thousands and thousands of times with people who are even trained in any kind of psychic training Herbility, they're able to affect the results of a random number of generators. So if they flip the coin For heads and tails people can actually affect the results with just their mind. Okay, this is proven thousands tens of thousands of times now If our minds can affect a random number generator from a software Would it work the other way around could software actually affect our consciousness? Good question, right? Well, the answer is actually yes And what we've done is we actually use software with certain programming to actually affect consciousness Pretty neat, right? So if mine can affect software software can affect the mind where we are all connected and everything in Creation or everything in the universe is connected whether it is living or not living whether it is biological brain or a Computer brain. Actually, they are still all connected pretty interesting, right? So that's why people You know all that future is says in future. We're all gonna be cyborgs because our brains are actually very suitable to adding You know cyborg implants and we already have cyborg implants now, you know Elon Musk, he has a company called Neuralink and what it does is it basically has implants a computer chip into your brain and then it uses a robot to Wire up all these electrodes in your brain in the right place down to like the smallest measurements and He can use that technology to basically make people who are Paralyzed to walk again. Why because now this computer chip is controlling the stimulus of the brain Special different parts of the brain. So our whole body is actually wired to become To interface with technology pretty Pretty efficiently. Okay, so what I've created is not like Neuralink Obviously, we're not gonna I haven't I don't have the money or technology to create something like Elon Musk But it's better because you don't need to put electrodes and put things into your brain and put a computer chip in your brain to Change the behavior or the change How well your body works? We actually use just frequencies because frequencies can go through the body can go through the Skull, I mean the bones that legumes joints and everything and you can use frequencies to program or basically Send data or send instructions directly into your nervous system directly into yourselves to tell that to do whatever you want so for example, I busted my shin on On Monday. All right, I was training kickboxing and I kicked the guy who was like 250 pounds I'm 150 pounds. So he's obviously a lot stronger and a lot bigger than me and his bones are a lot thicker than me mine So I kicked them right where my shin pad is a gap You know, you haven't wear shin pads supposed to protect your leg, right? but I kicked them right where my shin pad has a gap so I busted my leg and I couldn't walk So I kept fighting another four rounds after that. I you know, I I I took the pain and they kept fighting for four rounds. Okay But after that that night I couldn't walk. I had to use a cane because it's so painful. I was a It wasn't much swelling, but there was just a lot of pain throughout my entire shin and it spread to my ankles and and so on So what I do I use Chi energy AI and I use Chi coils Okay, and I use my red light therapy and then I went to sleep the next day I can walk without the clean cane So I can I can walk slowly still a little bit pain in my ankles and I couldn't move like when I Move my ankle and stretch this. It was still pain, but I was walking slowly the next day so that was Tuesday and then Wednesday, which is yesterday I put it on I I used the Chi energy AI Chi coils and I also used it used the Red light infrared therapy. I put my foot on here for about a couple hours and then that day I was 80% Okay, so I was walking normally. I was doing some stretches. There's still some Pain when I do some stretches in certain angles, but I was back to 80% now today I was back to training Okay, and no more pain a full range of motion and it was back training. I didn't even expect I was gonna be This good by today. This is Thursday. Just just four days later Okay, and no pain killers and no physiotherapy. I had obviously no surgery or anything like that I had no massage no acupuncture. None of that. Okay, all I did was just use energy Chi energy AI Chi coils and the red light therapy machine, which is all available on my store so basically in four days I healed a Severe injury from martial arts and I think a normally a person would probably take three weeks to heal this Normally, okay by themselves and they will be on pain meds for the whole duration of it probably okay, so I Was able to heal myself completely in four days So that was part quite a miraculous and sometimes I surprised myself with how well the technology works. Anyway Back to what I was saying. We've got some some Comments here. Can I develop my eyesight to be able to see clearly at night like a cat? What the red light therapy used for? Yeah, well, we have frequencies that kind of Help your eyes that you know support your eyes health and we have some Frequencies that can help you do that so you can you can use those frequencies with the coil and it can help you with your with your eyesight Programs with TV and movies and bad frequencies of radio. Yes The radio frequency on the radios are bad. Keep us in fear and anger Okay Oops. Why is it doing this? She closed good frequency. That's right Sissy says I watched my brother return to the gym three days after rupturing attendant on his leg He utilized a gold g-coil and frequency What can cure sacroiliac dysfunction? Can frequency cure it? So people will always ask me can my frequencies or can the cheekbones cure this cure that? I cannot make any claims that they can cure anything. Okay legally. I can't do that, right? But there are a lot of reports that frequencies and Also electromagnetic fields have a lot of benefits for various different things you can research it yourself You can just research just go PEMF And search for What do you call it? There's a like a database of all the scientific research and you can Search PEM PEMF and I forgot the website Come to my mind later, but you can even use chat GBT and ask like PEMF scientific Scientific research and it's gonna give you a whole bunch of stuff, right? Like for example, somebody somebody type in sec sacro Back this function. I'm gonna type it in chat GBT right now. It's function Is there PEMF therapy Okay, so I just asked chat GBT sacroiliac dysfunction is a condition that involves pain and discomfort of the sacroiliac joint which connects the spine to the pelvis PEMF therapy is a non-invasive treatment that involves the use of low-frequency electromagnetic waves to stimulate healing and reduce pain here are some of the PEMF therapy research for sacroiliac dysfunction Okay, so in 2013 a systematic review of meta meta analysis of PEMF therapy for knee osteoarthritis Found that PEMF therapy significantly reduce pain and improve physical condition compared to placebo Okay, so you can just ask chat GBT these things about PEMF therapy and you can find all the supporting Evidence that it works. This is not even me talking. This is just as I'm just reading it from chat GBT They'll be looking for answers about PEMF PEMF therapy. Okay What is the red light therapy So what I use is I use this panel, okay, you can get this from a website. So it has a Setting and on it you can actually set the frequency of The lights now there's two types of lights on here. There's infrared light, which is Invisible but see right now it's on It's supposed to be invisible as you can see a little tiny red dots You can see a little bit of glow like little pink glow, right? So this is the infrared and And it has a very very faint pink glow if you can see and Then it also has a red light, but I don't use red light because it's too bright. So I do is I turn on the infrared by the way, you can turn on infrared red light or or at the same time and You can select the frequency. So I selected 840 800 548 500 what I put in here 584 okay, 584 you can go from 0 to 5,000 and that's one frequency is good for Going deeper into the tissue. Okay, so there's different frequencies basically the higher the frequency the deeper it goes So 584 is is good for your skin good for a little bit deeper than your skin and your tissues That's why my my skin is so beautiful right because I use this every day actually I put it on my Desk and I'm working. I just put it like this working on a computer I can still open my eyes because the lights are not blinding and I put it on one side of my face I do 30 minutes and I do put the other side of my face and do 30 minutes and it's actually quite easy to use And then when I used it on my foot I just I just put this down on the ground or on on the chair and then put my foot on top of here on top of here and And afterwards actually the pain Went down dramatically. So I use this all day today this morning and yesterday together with the qi quality energy I and so on and you can feel the heat by the way So you can see a little bit of pink light coming in from it. This is the infrared and I can feel the heat so that heat is our light waves that are Below your visible range and it goes deep into your body. Okay, so that's what infrared is since somebody asked what it is and It's actually quite amazing what this thing can do. So so that thing That light panel I just showed you they make these beds that use the exact same LEDs except they're a lot bigger that one has about like 30 or 50 lights So they get make these LED beds and they have let's say like a few hundred lights Those are $80,000, but this one is only $500 so you get in the same type of lights and same intensity of Lights for a fraction of what it will cost if you get a red light bed So all the stuff like I really researched a lot like other than that Chico's that we make ourselves we we source these red light panels and we've tested many of them and we found the best one Hey, um Hey Carrie he's older than 18 and he's very Yeah, so I'm not 18 years old. Do you guys think I'm 18? So if you look at my videos actually Like last year I look younger now than I did last year I actually Checked it out. I look younger Today than I did last year or two years ago. It's pretty amazing. So and it's because of all the stuff. I've been using And what is I'm gonna say so I'm actually 42 I'm not 18. I'm not 28. I'm not 38. I am 42 Yeah Yeah, David is reverse aging Yes, so everybody if you if you look at people's social media accounts and for people who Use cheap oil she energy I and all that our stuff Look at their social media accounts See when they bought the cheap oils or started using that our product and then look like go back Like six months go back a year and see how they look they completely different. They look completely different They look younger. It's crazy. Okay, so we have documented evidence To show that all our technology actually would helps you to age backwards pretty crazy. I love this suit. Thank you Yeah, it's pretty cool Yeah It's pretty I really like it because it it's a white and then it reflects my my lights pretty cool, right? So he's got this shiny silver Pattern on there and when you put the light it lights under it reflects the color. So it looks like I'm glowing Pretty neat, right? Do you ship to the USA? Yeah, we totally do. We have a lot of customers. Yes. Hey, would you say you ship globally? So if you anywhere in the world, we will ship to you so When people start using the cheap cause They have to kind of understand how the technology works because there's just so much stuff We've developed over the last five years So it could be overwhelming about like how all these frequencies work How all these different curls work how the software where our satellite therapy works. There's just so many different Methods of getting frequency therapy or getting Chi energy so Maybe I'll give you some rundown of kind of the different Types of Chi energy or the different types of frequencies there are so first let's start with this basic thing Okay, it's the sound frequencies a sound frequencies You can play with headphones you can play it from your phone You can play it from your computer you can play it from a speaker you listen to them Okay, and then your body and your mind will respond to these frequencies that you listen to and we have eight of these that are Free and you can download them go to use the chi calm All you have to do is listen to them. That's all you need to do nothing else And you can meditate with them you if you want you can listen to it while you work out Doesn't matter as long as you listen to them. It's going to affect you. All right. What kind of effect do you get? Well, some of them will help you calm some of them will help you sleep So I'm doing help you feel more energy some of them will help you focus or help you to meditate or attract Good luck into your life. So that's the sound frequencies. That's a basic right then the next level is Something called cheek oils. So what happens is when you use cheek oils? Okay, now turns the sounds Into magnetic energy. So here's a set of cheek oils I'm not going to explain too much how how they work and everything but basically the sounds go through This power supply Connects to the coil now the coils we emit energy because the sound are now converted into Magnetic energy okay magnetic and what do magnets do magnets are invisible and their energy goes through everything, right? So that's how the coils work. So the sounds became magnetic energy So when you get the coils, there are no sound coming from the coils Okay, because they're turned into magnetic energy. So that's the first. That's the second type of coil if we can't avoid the coil What else can we do to heal with sound? Well, yeah, we have many different frequencies You can't afford the coil by the coil you can do financing so you can get it for like 50 bucks a month or something You know our lowest model the coil start of three hundred ninety nine dollars So you can get it for something like 60 or 70 bucks a month stuff like that But if you still don't want to do that, you just want to do the frequencies We have lots of frequencies you can use as well My co-worker his voice just irks me can't deal with frequency of his voice. What can you do? That's a tough one Maybe you can put some headphones in and listen to some of our frequencies. So you don't have to listen to his voice If you can't afford Cheat coils you can use your frequencies and frequencies, you know You have some free ones and then you can get some other ones for like anywhere from twenty dollars to a hundred dollars or even more So that would be less cost in the coils But like I said the coils are not expensive if you finance the coils. Okay, so the third level is something called Chee energy AI and with that it uses software or uses AI to Send energy through your phone and there's no sound. There's no lights. There's no video to watch. There's no mp3 There's no music or anything. It's completely silent. You can't see anything. Okay. All of this is it Is this just pure energy? Okay, all I can say is this pure energy you turn it you can turn it on and off when you turn it on Basically, you can feel the energy coming out of your phone as if as if you just drank a coffee But you didn't drink a coffee Okay, or as if you just had an energy drink, but you didn't have an energy drink you can feel it actually energizing you and It's pretty bizarre and a lot of people use this and they're totally mind-blowing. So how is this working? It's just I just turned it on and then I'm feeling this energy in my body without Taking anything without exercising without doing any breathing without meditating. It's just happening right there Okay, so that's the third level and that's called Quantum we call it kind of quantum entanglement call it quantum energy and So that's another level energy and if you and that's really affordable. So that one is free to use And you can start with a paid paid version of it for only $15 a month. So If you don't have that much Money to buy some of our equipment then the Chi energy AI is a really really good Way to start experiencing Chi energy for you or even your whole family. So Jamie I would suggest Going to Chi energy AI and check it that out You can get a free account that you can test everything out We have one energy signature and you can use it for eight hours at a time So that's totally free as much as you want and if you just want to use that forever and don't buy anything That's fine, too. Okay, but eventually you can say oh, I'm gonna I want to send it to my home You can send it to an address as well and I want to try some other frequencies Not just the one frequency then you can pay $15 a month That's it and you can use as much as you want. Okay, so it's very very affordable and the reason I made it is because I want Many many people mill a million people to experience this Chi energy. So go check it out I Wish you have a mini of those products a Mini yeah, we do have a Chi call mini We have a Chico mini and if you don't know what it is Is this one here? Is the small one? Actually, it's not this. This is not small. Okay. This is the mini but people make I've seen stuff that have are even smaller but But this is actually pretty big compared to Stuff out there. So this is really big and it's really powerful Okay, so this is the mini but don't get deceived by the word mini because it's a very very powerful. Okay Quantum entanglement is in the store quantum entanglement is on Chi energy dot AI I'll probably have to put up a link on the store so it can check link to there directly Gene energy I If you go to use the chi calm, it'll have everything. Okay. Yes, you can see the link to the Chi energy demo How much is the smaller one the smaller one is three hundred ninety nine dollars? It comes in the whole kit whole sets and I'll show you so that's the Chico mini comes in the whole sets And it has all this in here and it comes with the Seven of the basic frequencies for free and if you subscribe for Ten dollars a month, and you get ten thousand right frequencies that you can use with it Okay, how do you get a hold of me? If you need to get a hold of me, you can just send me a message on Instagram If you have a question about product our customers


All right friends, so here is a question on rigid body motion. So let's see what it is about Now two identical particles moving towards each other with velocity to V and V respectively We need to find velocity of center of mass. Okay, so this is a system of two masses. Okay So there are two masses I'll draw a straight line first Okay, so there are two masses. Let's say this is one mass Okay, there is this another mass right both are identical the masses are small m Okay, one is moving with to V and other is moving with V and it is written that they are moving towards each other So this must be the scenario Okay, now velocity of center of mass we need to find out so center of mass of what center of mass of these two masses Okay, so we know that the formula for velocity of center of mass is M1 V1 Plus M2 V2 Divide by M1 plus M2 Okay, now many a time students think that this is a scalar formula. Okay So that is not true. It is a vector equation. Okay, you need to take care of the directions also Okay, but then if vectors are aligned in one straight line, you can treat them like scalars But again, you need to make sure that vector in one direction is positive vector in other direction is negative Okay, so I can say that this direction is Positive and that direction automatically becomes then negative. Okay, so we see M will be equal to M into 2 V Okay, plus M into or minus V this time. Okay divided by M plus M Okay, so you will have V by 2 as the answer Okay, that is how you have to solve this particular question. Thank you


Should you cut out your existing friends because you no longer drink and they do and they're corrupting your mind? Well, when you quit drinking it is very possible that you will lose some friends. You may proactively choose to cut them out of their life or to just move on or you may just find that you just naturally gravitate somewhere else. So yes, you will lose some of your existing friends possibly. You don't have to. You don't need to. Because when you are alcohol-free and you're feeling great in that choice and you're powerfully drinking water and soda water and you're feeling great and you're awesome, it doesn't matter what social group you're in at all. You can be a chameleon. You can like float into a group of people who are drinking heavily and you can just powerfully have a great time while not drinking. You can float into another group of people who are not drinking heavily, who are drinking only on occasion and you can still remain alcohol-free and love it and enjoy it.  I'm James Swanig from the Alcohol-Freedom Formula. There are links down below to help you get started on your quitting drinking journey. When I quit drinking in 2010, did I lose some of my friends? Not initially. Did they challenge me on not drinking? Yes. I had one friend, one of who I told him that I was about 20, 21 days not drinking and he said, Do you want to drink from the bar? I said, Yeah. He said, What do you want? I said, a soda water. He goes to the bar. He comes back five minutes later. He gives it to me and then I look at him and he's got this little smirk on his face and just before I sipped it, I was like, did you put vodka in this? And he went, Oh yeah, I did. I was like, well, you don't want to know what I said to him then, but I wasn't happy. And so you will find people in your life who will do this. They will literally not understand or comprehend that you are not drinking and they may try to like sneak alcohol into your drink like my friend did and you might be a little bit irritated by that or a lot irritated like I was. I get it. Now, I didn't make a conscious choice to cut that friend or cut the friends that I had. I just continued on my alcohol-free journey and what happened was is that I started naturally attracting a different type of friend into my life. I just started to gravitate towards people who didn't need alcohol to socialize. I started getting into health. I started getting into a little bit of meditation. Some years later, I got more into that, but at the time I just kind of dibbled and dabbled in it and I did start going to the gym. I ran a half marathon. None of these things are excessive. None of these things are like addictive, like physical behaviors. Like just because I give up one thing, I'm now going to go and be addicted to like this health. It was just a natural kind of beautiful flow to starting to be attracted to healthier choices and attract people who made healthier choices in their own life. So I didn't consciously say I am no longer hanging out with this group of friends and that's it and that's done. No. No, I didn't do that. I just kept hanging out with them, but then started spending more time or increasing amounts of time with this new group of people that I hadn't been exposed to previously. And I didn't even have to go and seek them out. They just found me because when your energy is health and presence and you're into growth-minded things, you tend to just attract people who are into health and growth-minded things. It's just one of the laws of the universe, right? The law of attraction. So should you cut out some of your friends? Well, the word should is a story, right? So should we? Yeah, maybe. Maybe some of them don't serve you. Maybe some of them are draining you.  And if that's the case, that's okay too. I'm 43 years old as I'm recording this and I can tell you that the friends I have today are different from the friends I had just five years ago. People that I may have hung out with for a year or two, five years ago, some of those people I haven't seen in that amount of time, not because there was a falling out, not because anything dramatic happened, but just people just go their separate ways. And that's life. And that's okay.  Maybe or maybe not. That's a choice you have to make. Is it okay if you deliberately cut them out? Yes, that's okay. Is it okay if you don't cut them out? Yes, that's okay. There's no judgment around this. There's no right or wrong. But if someone is draining you and not serving you and you consciously want to go, not they're cut, I want to move on, then do it because don't worry. There's a whole new beautiful social group here waiting for you. Gone are the days where if you were ostracized from one group that that meant social isolation. No, now we have Facebook and meetups and Instagram and a way of connecting to people. I don't mean like connecting to people online. I mean using those online platforms to connect to people where you can go and meet them in person. So to be clear, I'm not saying cut your friends and go and join social groups online. I'm saying use those social groups online to connect with people who you can meet with, meet in person. Now just now I'm filming this right now. The camera person who's filming this right now, Ryan, is a health conscious traveling person. Show us. Turn the camera around. There you go. Good on you, Ryan. Okay, come back. Ryan is a positive, healthy guy who's out traveling the world in Bali doing cool, epic stuff. There's a reason why we attracted each other into our lives, why he's filming me now doing this, why he's into this. Ryan did my 30 day no alcohol challenge program back in I think 2015, right? Flip the camera around. That's correct. 2015 you did it? Yeah. So you found me online and why did you do that? Why did you find that group? Flip it around again. You know, it just kind of found me through one of those social circles. There you go. Spin it around. So people are just attracted to people who are on the same frequency. I had a program called the 30 day no alcohol challenge which helps people quit alcohol and Ryan somehow found that program and did the program and then quit alcohol. Yep. So when you do that, you just naturally start to find new groups of people.  Maybe. Is that scary? Yeah, I get it. But will you be attracted to and find and naturally be magnetized toward the new group of people who bring you up and energize you and push you and hold you accountable and you get to learn new cool, epic stuff. Yes. So yes, you will lose some friends. Maybe either proactively you'll make the cut or maybe it'll just naturally happen and that's okay because you are on a path to growth. You're on a path of health. You're on a path of presence.    Thanks for listening to the alcohol free lifestyle podcast. I want to load you up with some free stuff right now. So if you want to go to JamesSwanick.com slash guide, I will send you my quit alcohol guide which has helped six figure entrepreneurs and top professionals produce or quit drinking. You can also text the word quit guide to the number 44222 if you're in the US, of course, it doesn't really work anywhere outside of the US. But if you're in the US on your mobile phone and you'd like that guide, text the word quit guide to the number 44222 or you can go to JamesSwanick.com slash guide. If you'd like to schedule a free 15 minute call with one of my top coaches, just an exploratory call to see if or how we can help you, then you can go to JamesSwanick.com slash schedule or you can text the word project 90 to the number 44222 if you're listening in the US on a mobile phone. That's JamesSwanick.com slash schedule or you can text the word project 90. That's one word project 90 to the number 44222. Feel free to send me a direct message over on my Instagram account, which is at JamesSwanick. You can also watch video episodes of this podcast and a series of other educational videos on my YouTube channel, which is JamesSwanick one, or you can direct message me on Facebook at JamesSwanick official. And finally, a request, would you please now write a short review of the podcast inside of the Apple podcast app on your phone or on iTunes on your desktop computer? Would you please give the show five stars and write a quick one or two sentence review? This will help the show get in front of even more listeners potentially transforming someone's life. You can rate and review the show inside of your Apple podcast app on your phone or over on iTunes on your desktop. Thank you so much and I'll catch you next time.


I've titled my remarks resurrecting our buried prosperity. Resurrecting our buried prosperity. As of 1940, Nigeria was already globally a leading producer of tin, columbite and coal. Potential coal reserves are today estimated to be as high as 3 billion tons. Much of our coal is high-grade, clean and bituminous with a low sulfur and ash content. But even farther back in 1913, gold in commercial quantities had already been found in parts of northern Nigeria and what is now Oshun state. Bitumen was discovered as far back as 1900, mostly in the southwest zone, the bitumen belt covering what are now Oundo, Ogun Lagos and Odo states. And today we have deposits in excess of 42 billion tons. This is the second largest bitumen deposit in the world, and almost twice are all reserves. How about iron ore? We have the 12th largest iron ore deposit in the world, more than 3 billion tons. Besides, the Nigerian Geological Survey Agency confirms that Nigeria has over 44 mineral deposits in commercial quantities in over 500 locations across the 36 states and the federal capital territory of Nigeria. And these minerals range from industrial and energy minerals such as iron ore and coal and gemstones including sapphires, rubies and emeralds. So buried in the ground in various parts of Nigeria are riches that could ensure that no Nigerian is unemployed, underfed or neglected. And this is not mere speculation because before the discovery of oil, Nigeria developed its infrastructure, its roads, its wells, its dams. It provided free education in the western region, for example. From the proceeds of minerals, agriculture and taxes. But with the discovery of oil, these incredible riches have remained buried in the ground for decades. In many parts of the country, you find illegal miners with poor equipment, essentially scraping the surface of these deposits. Some better equipped operators are able to do more, but they largely just exploit cheap labour and pay next to nothing in taxes or royalties and of course cause environmental damage that is never remedied. So it is clear that unless we get the management of our solid mineral resources right, we would continue to live in the terrible paradox of suffering in the midst of plenty. The problems are numerous, but they are solvable. The principle issue is that of the challenges of implementing the regulatory framework. While the Ministry of Mines and Steel has the legislative mandate to regulate mining in the country, many states and local governments have embarked on the imposition of their own rules and regulations on miners in their states, including issuance of registration, permits, community development agreements and memoranda of understanding on miners or with miners. Some state governments in a bid to shore up their revenues impose illegal fees, taxes and levies on foreign and locally licensed and local licensed mining companies and operators. Enforcement of these taxes often leads to the frequent arrests and harassment of licensed miners and their workers and of course closures of mining sites and this happens frequently. Regrettably, such actions by state governments constitute a major disincentive to prospective investors in this sector and invariably jeopardize the efforts of government to actively deepen the mining industry in Nigeria. There's no way this will ever benefit the majority of our people. Then there are of course the huge security concerns that always develop in the struggle for control of mineral resources in poorly regulated or unregulated or ungoverned spaces. Almost invariably, these situations lead to criminal activities and particularly a proliferation of weapons and armed groups and this is true historically and is even more evident now. On the African continent we have examples of how resource extraction can trigger an increase in criminality. In Nigeria, armed banditry, hiring and equipping of armed groups by illegal but powerful mining concerns in many of our resource rich areas is clearly fueled by the chaotic regulatory environment. So it's for these reasons that this strategic engagement with the leadership of the 774 local governments is critical. It is apt and timely especially as a federal government through the Ministry of Mines and Steel seeks to fully implement its robust and wide-ranging remit in policy and legislation. This engagement will certainly help in explaining the regulatory framework to the governments at the community level where most of the mining activities take place. It will enable the Ministry and other stakeholders to get direct feedback from the local governments and those who have to deal with these issues on a daily basis. But where are we today? In 2016 the Ministry of Mines and Steel Development in collaboration with industry stakeholders formulated the roadmap for the growth and development of the Nigerian mining sector. The roadmap addresses some of the tensions between the federal government and sub-national entities in relation to mining. The roadmap which identifies partnership with mining communities as one of the quote critical levels of success states that success in Nigerian mining requires partnership across multiple communities, stakeholders and institutions end of quote and also stresses that the role of state governments as both co-investors and sector champions is critical. It further asserts that a sustainable growth model for the sector can be found only through government innovations and partner actions. For example, community engagement mechanisms, shared investments and co-creation opportunities. Section 19 of the Nigerian Mines and Minerals Act establishes the Minerals, Resource and Environmental Management Committee, MIREMCO. In each state this committee is composed of representatives of the federal, state and local governments and is designed to serve as a platform for fostering synergy in mining operations across all tiers of government. Consequently the Act accords communities the status of co-investors in the development of mineral assets. So the Minerals, Resource and Environmental Management Committees are important in bringing order, fairness, inclusion and equity to mining and its value chain. Mr. President has also mandated the Ministry of Mines and Steel Development to prioritize the orderly and efficient development of seven strategic minerals in the mining roadmap. Namely coal, bitumen, limestone, iron ore, barites, gold and lead and zinc to support local industries and for export of processed mineral products. The Ministry was further mandated to strengthen synergy with the state governments and relevant security agencies for the enhanced security and safety of mining areas. The Ministry is also expected to foster collaboration with the private sector in growing the mining industry in order to create employment opportunities. Recently also as part of our COVID efforts to preserve existing jobs and create new opportunities, 6 billion Naira was approved for the mining sector under the current economic sustainability plan. And this is for the development of mineral processing clusters across six geopolitical zones of the country to promote large scale investment in mineral exploitation. It's gratifying to note that the Ministry has recorded considerable progress in the establishment of these mining processing clusters and on security in mining areas. As part of our broad efforts to re-engineer the national security architecture to more efficiently confront our challenges, the President has recently approved a raft of measures aimed at ensuring the security of mining concerns of mining sites. These measures will be driven by the Office of the National Security Advisor in collaboration with the Ministry of Mines and Steel Development and we believe that this will be hugely significant in creating a safe and enabling environment for the growth of the sector. A few things emerge from the regulatory and policy framework that I've just shared and which of course you've heard quite a bit about in the earlier statements that have been made. And I will also make some suggestions. The first is and this is number one is that mining is constitutionally under the federal government of Nigeria. The second is that mining revenues go to the Federation account and is shared between the federal government, states and local governments. States where resources come from get a 13% revision in addition to their share. The third is that the federal government cannot effectively regulate mining without actively and deliberately working with states, local governments and mineral producing communities. Number four is that the federal government, states and local governments have to jointly develop working arrangements to ensure that mining is attractive to investors, profitable for states and local governments. Not just from proceeds of mining but also from the personal income tax that will be paid to the states by mine workers and the staff of mining companies. The fifth is that the communities where mines are located must also have some direct benefits aside from jobs for their residents. We must find a formula for compensating the communities for environmental degradation and erosion. Number six is that there must also be a framework for ensuring that environmental degradation is remedied. Number seven is that we must discourage the export of raw material, raw minerals beg your pardon. Refining, processing or beneficiation is the only real way to maximize our mineral wealth and create good paying jobs and opportunities for our people. The seventh is that this engagement today should lead to the production of or the fine tuning of a clear working document that spells out with clarity the roles and responsibilities of each party, that is the federal government, the states and local governments and the mining communities. Even where these roles and duties have already been clearly defined in existing legislation or regulations, this is an opportunity to simplify the language, explain the relationships, take inputs on implementation concerns and get everyone's buy-in. We must commend the Ministry of Mines and Steel Development, the Honourable Minister and the Honourable Minister of State Mines and Steel Development for this initiative. I think the Ministry has been very fortunate in having able, dynamic and farsighted ministers since we came into office in 2015. Well done indeed. I can only encourage you to continue to approach your role as the regulator and administrator of the nation's mines and steel sector with open mindedness and flexibility, especially in your collaboration with the states and local governments. I must also urge all officers of the Ministry to rededicate themselves to the realisation of the set target of a 6% contribution of the mining sector to the nation's GDP by 2025. Finally, let me say to all participants that this engagement is one that could be a game changer for mining in Nigeria and it could mean a new era of huge earnings and prosperity for our people from our mining resources. But this will only happen if we adopt a collaborative and cooperative approach. If we work together, we will reap far more than we could ever gain by working alone. And so it's now my special privilege and pleasure to declare open the mid-in addition of this strategic engagement on sustainable mining by the Federal Ministry of Mines and Steel Development with Nigeria's local government chairman and other critical mining stakeholders. I wish you all fruitful engagements. God bless the Federal Republic of Nigeria. Thank you.