And it was her lifelong dream to do this race. And after that accident, I promised myself, some point in life, I don't know when.  How are we doing today? Everybody, hope you're doing well. All right, another day, three miles today. I already ran 5K, I think I was 820 a mile, approximately, so the day after Amsterdam ran 1115 a mile in Amsterdam and through the canals. It was actually really, I was in a lot of pain, but it was really fun to just go explore Amsterdam in those early morning hours. And then yesterday was 10 minutes a mile for three miles. And today was 820 a mile. And so yes, the legs are coming around. It's very exciting. And yes, it connects to the big announcement today. Oh my goodness. Okay, question of the day, hit pause.  Hit pause, guess down below what is my next marathon? Now, no cheating, don't read, well, okay, yeah. What is my next marathon? Just go down below in the comments. That's the question of the day. That is the big announcement today. And, but you have to listen to today's vlog so it all makes sense because it's a big long story. It's an important story to my life. And I'm excited to share it with all of you. But if you don't listen to the vlog, you'll be shocked and confused and not understand. So anyway, just wanna put that, put that out there. Oh my goodness. All right, we're heading out everyone to the gym and then to a massage. Just maintenance, maintenance, maintenance. The legs are coming around already, which is exciting, but a lot of you, it's just like those little things every day, including the foot log. So I've noticed recently that quite a few folks are buying this, it is available down below from Amazon. I use this for, I use this most especially to help get rid of my plantar fasciitis in 2018. And there's a lot of different recovery tools out there that are similar to this. I like this particular recovery tool because of the exact diameter of the foot log, but it's these little, I'm not gonna like points, these pokey points that basically help relieve little, little tiny pressure points on the bottom of my feet. And I use this every single day. But in addition to massaging, I'm gonna show you, I also just stand on it, like you see on the screen right now. And that I think, and based on the research I've done is actually more important than actually massaging your plantar fasciitis issue, like right on the heel. You know, it's very tempting to massage right on the heel. And I have learned based on my research that that is not the best strategy.  And how I do it, slowly, gently, work into it, ease into it, is standing right on top of it for 30 seconds on, 15 seconds off, 30 seconds on, and do that three, four, five times. But you have to build up to it slowly. So anyway, thank you for ordering the foot log and messaging me about how it is working or not working for you, definitely let me know. But so far it seems that quite a few folks are using this right now. Okay, let's head out.  And before I forget, so far no one has guessed my exact time for the Amsterdam Marathon. Someone was two seconds off, very close, and I've made it through about two thirds of the comments. There was about 2,000 comments, so so far I have not found an exact time, oh, hold on, I have not found an exact time. But if I do, I of course will let you know. So I am still digging through all the comments on the guesses, and the winner, if somebody gets it right, is gonna get a new free pair of brand new running shoes, but no luck so far, so anyway. All right, let's go get this body right. We'll see you in a little bit. Good job with your spaceship. Oh, a jet. All right, we're heading to the shooty-o, everyone. The cold shooty-o, time to pull out the heater now. Where's that space heater at? It's time, everyone, the heater is on. We are heating up the shooty-o here, trying to stay warm. I'm just reflecting on life, oh my goodness. I was actually just glancing back at the Cleveland Marathon map. I think I'm gonna keep that forever as a reminder. A reminder that life has a lot of twists and turns that we often cannot anticipate. A lot of challenges, highs and lows. The curve ball, right? I love that picture, that analogy, like life throws us curve balls. And I'm only 34, but even since college, I've learned a lot about how life throws us curve balls. And it's really how we react to the curve ball in our swing, which will determine the outcome of our success.  Cleveland was one of them with the injury. And it was hard to accept in that moment. But because of that injury, a lot of doors were open, including, yes, the Amsterdam Marathon. There's my bib number, gonna keep this forever. I'll write my time and date and location, of course, on the back here. That's what I do with all my bib numbers. So I'll put 223, 37. I think I ended up about 26th place, which is pretty neat. But anyway, so, but because of Cleveland, I ended up in Vienna to film Kipchoge. I ended up in Amsterdam to run my, to race my first marathon.  I think that determines a lot of our success. So what I'm about to share with you is that it's hard to talk about. It's, you know, it's just hard. It's a hard thing and like, oh, we're dealt a difficult deck of cards sometimes. And so I feel like my mom has been dealt at times a tough deck of cards and she's a runner. She's been running her entire life. And it's, I just, it just makes me a little upset at times when things don't go her way, including this dream that she had to race this one particular race here on this earth. And so the situation is that I'm now signed up for my next marathon. And it happened in a way that I can really explain, but it happened and I'm beyond grateful. And it happened because my mom, I told a story here on the channel one year ago and a vlog fan heard this story now, really a vlog friend. And I'm, she's gonna remain anonymous, but she knows who she is. And she, you know, did some work on her end and made this happen so that I could register for this race. So if you didn't hear this story a year ago, basically it's a tough story and I'm not gonna go into too many details, but my mom, she's a lifelong runner and she really taught me how to love well, how to be patient with others and how to persevere through a lot of, I'll just, I won't use that choice of words, but through a lot of challenging things on this earth, okay? And one of those challenging things was that she registered for this dream race that I'm now registered for 10 years ago.  And so don't drink and drive, all right? Out there, you know that, you know that. And it shattered her ankle, messed up her knee real bad, ended up in a wheelchair about two months before this race. And it was her lifelong dream to do this race. And after that accident, I promised myself some point in life, I don't know when, but at some point I was, I was, I would have been about 22 or three at the time. So maybe it was over 10 years ago now, but I said at some point I'm gonna run this race for my mom, all right? So that's the backstory to what I'm about to tell you in nine days from now, I will be racing the New York City Marathon, which don't try this at home. That's why I put that in the title of the vlog. Don't try this at home. You don't do this. You don't go race two marathons, even you usually race two marathons a year, okay? But because this vlog fan and friend really pulled through and made it happen so that I could get into the New York City Marathon, which I found out about six weeks ago. I didn't tell all of you because I wanted to focus just on Amsterdam and obviously Amsterdam is a flat, fast course. New York City is not a flat, fast course, but I'm going out there not to try and save my legs, obviously, not to try and run as fast as possible. I'm going out to the New York City Marathon to race it for my mom, okay? Love you, mom. If you're watching, you'll watch this at some point. And so that is what's happening. That is the big announcement. It's a little insane. I realize it's just a little crazy, but I'm doing it for my mom and I'm so excited.  So she grew up in New York just so you know, that's why she loves all of her family is still in New York. And I was basically raised in New York during the summer times. We would go out to New York all the time. So anyway, we're gonna do a meetup run in New York City. I will post the details very soon on Strava and I love you. And that's the story. That's the big announcement. I know it's a little crazy. And bottom line, I'm not afraid to go out and try to live life and live life for others. And I hope my mom can experience the New York City Marathon through me running in nine days from now. And okay, I guess I'll just mention like, this is a once in a lifetime opportunity. People apply their entire life and never get into the New York City Marathon. I think I've heard it's like a half a million people apply every year now. It's a lot. And I believe it's like 55,000 they accept. At least that's what I heard last. Maybe it's over 60 now, but it's a lot of runners every year, but it's like people never get into this race. So I am not passing up this opportunity. I know it's not ideal timing, having just done Amsterdam, but I'm taking care of my body, getting massages, eating well, going to the pool, doing all the little stuff.  All right, so I love you all. And onward and upward.  Okay, it's crazy. I know it's a little crazy. Again, don't try this at home. I don't recommend this, don't recommend this, but I'm gonna do it and it's gonna be amazing. Ah, so that is that. I already asked the question of the day. Did anybody guess New York City? I'll be curious to read the comments down below. And all right, onward and upward. I already said that. We're gonna toss it back too. Yes, the Amsterdam Marathon race vlog on the right. And we'll go on the left. We'll go maybe back to a Cleveland Marathon vlog when I was training for the Cleveland Marathon since we talked about both of those races in tonight's vlog. I love you all. There you go. All right, seek beauty, work hard, and love each other. See you tomorrow.


Marsha Joyner and we are navigating the journey. Navigating the journey is dedicated to exploring the options and choices for end of life care and to assist people to talk about their wishes. It's time to transform our culture so that we shift from not talking about dying to talking about it. It's time to share the way we want to live at the end of our lives and to communicate about the kind of care we want and don't want for ourselves. We believe that the place for this to begin is not in the intensive care unit. Together we can explore the various paths to life ending. Together we can make this difficult conversation easier. Together we can make sure that our own wishes and those of our loved ones are expressed and respected. If you're ready to join us, we ask you. Navigate the journey. As you know, we are supporting legislation to have death with dignity or medical aid in dying made legal in Hawaii. The Senate bill is being heard at the Capitol as we speak. Therefore I have invited Walter Peters, of course, a dear friend, who has experienced the real turmoil of the end of life of a loved one when that person had no options or choices. Today's guest, Walter Peters. Walter? Hi, Marcia. Thank you so much for coming and thank you for being willing to talk about something so sacred, so close to you. Sure, you're welcome. So tell us a little bit about you first. Oh gosh, well. Okay, a big bit then. The Reader's Digest version. So I was born in Santa Monica, raised in Southern California, Hawaii. I mean, Beach Boy, right? Surfing, hiking and all that stuff. And I bought the publicity when they said LA is the place. I was like, yeah, it's the place, the mountains, the ocean, the forest, above it. But then when I was in the Navy, I came here and I was like, they lied. This is the place. So I moved here in 1991. Still the best decision I've ever made in my life. I just love living here. I own two companies. One is a small remodel and repair business. I fix people's houses and the others are coaching practice. So I consult with people about their mindset in the areas of sales or maybe executive management, things like that. Yeah. Okay. And I love to cook. So that's my thing.  I first six people that say, yes, that's my dinner party. Very good. Very good. You have special recipes you like? I make them up.  Come up with something new. Very good. That's great. Okay. I'll be there. Now, I've known Walter for what, four or five years now? Well, let's say it was 2012 we met. Walter and I sat next to each other at a dear friend's husband's funeral. Right. And that's how we met. That's right. And so here we are again talking about the end of life. Walter's sister, is this correct? Your sister was terminally ill and she had all kind of stuff. So I want you to tell us all about your sister.  She was born when I was 12 and I was in a war with my mom and my mom was winning, but I was still fighting. Right. And my sister was born and I was just fascinated with this infant and my mom goes, oh, this is your job now. Right. And so at the age of 12, now I'm dad. Right. But I was not my own. I had support and all that, but it just transformed me. It really changed who I was being in life. And so she was there for me throughout my life. I was there for her throughout her life.  But for whatever reason they didn't operate immediately.  It turned out to be the part that controlled her left leg. So she went from being this vivacious, brilliant, active young woman to a wheelchair bound patient for the rest of her life. And so she was that was when she was 42. Oh okay. So then she she seemed like she was recovering. And then in Christmas of 2015 she she sneezed. She had a pain in her shoulder and she hadn't gone in to have it looked at yet but she was going to. She sneezed and snapped her collarbone because there was a tumor there. So they went through the whole treatment protocol again radiation chemo all the things that they do for bone cancer. And again you know she seemed to be recovering. She was getting better. I still have on my phone a voicemail that she left for me in July. And she was singing to me she goes I have no pain I have no pain this is your sister and I have no pain. And that was in July well in August she and her husband were getting in the car and she her left hip collapsed. And she just you know she heard a snap and whatnot. So instead of going to the show they were going to go to they went to the ER instead. And they did all their scans of what it was. And they said well you have a tumor in your hip. And it's so deep and for us to go in and operate on it is not necessarily safe because there's so many complications that could occur. And we can't do any of the treatments that we've been doing on you because we've depleted your body's immune system so much that there's barely anything left. So for us to do chemo and radiation would be probably lethal. The operation we have no way of saying to you that it's going to be safe or that you'll survive it or that it will even work. So you know you need to go home and and just be ready to pass. And so she did and that was August and you know we were in communication I talked to her in September and I told her I was getting things together to be able to go up there and be with her and she said well hurry up and get here. And that's that was a shocking thing to hear her say because I knew that she was telling me there's not much time left. So you know I unfortunately have a great community of whom you know. And and so people pulled together and I got on a plane and I was there for the last month of her life. And you know the hardest part of it for me was just watching her deal with what she was going through because you know it was very painful to have a really deep break inside the bone. So they had all these different medications one the primary medication was for the pain but then she had three other medications to deal with the side effects of the medication for the pain and they all had other side effects.  And she's you know in discomfort and she's of course grumpy because she's been in a wheelchair for two years already right. And yeah she's like irritated by the smallest things right. And and tremendous discomfort in her digestive tract and her breathing was bad and her throat would get congested and it was just hard to watch her struggle. You said she was a 42? She was 42 when they found the tumor in her skull. Right. She was 44 when she passed two years later. She had that torment for for two years. Yeah but it wasn't always that bad right. I mean but whenever the the treatment was going on it was it was it was just horrible you know. I mean and so yeah you know there I was and I just was about how do I be of service here. How do I support her to get to whatever it is that's next for her.  In fact she and her husband shared with me that before I got up there in October she had gone to see one of her oncologists and during her visit he turns and he looks at he goes you know I can't believe you're still alive. Now a doctor doesn't generally say that to you right. But that's was he was like how have you lived this long. And it was that was the thing she was like I don't know doc. But I think it had to do with the fact that she was very active very energetic. She was that she danced you know she was a professional dancer for a while she she was a public speaker. She was a very high energy type of person and she always always really committed to organic foods and all those types of things. So I think she had a good body going into this. She had done such a good job taking care of her body that it was in pretty good condition when these other things happened and it just it was just going to it was going to last a while. Well you know when you mentioned what the doctor said I've talked to so many patients since we've been doing the show and they tell me that the doctors have no idea what to say. Right. Just they don't know. Yeah. Now one thing that one thing that complicated her situation where she and her husband live is 40 miles east of Ogden Utah in a valley in the Wasatch Mountains. So her hospice team is responsible for an area of about 250 square miles. Right. So they have just one it's one team. It's a physician a nurse practitioner and three others who I don't remember their titles but they would all come and visit periodically. Right. Somebody would be at her house at least two or three times a week. Right. But they would say well we'll be there at one o'clock but the truth of the matter is wherever they were before if it took them a little longer there's nothing they can do they got to do they got to serve that client and then they're on their way so this time they wouldn't get to her place till five or six thirty. But that wasn't the issue. The issue was that because the team was so spread out and only certain people on her team were empowered to actually make treatment decisions for her that it would take there was a delay between when a decision was made and when it got implemented and and towards the very end you know they they they modified her medication like three times in two weeks while I was there and but there wasn't any way for someone to be there to monitor the impact of the new medical the new medication protocol they put her on like to see how's that working yeah right because she was at home it wasn't she wasn't right facility so that that complicated things and it was it was it was hard for her it was really hard so but but she was given regular sedation and pain meds they had pain medication you know I mean she was uh when I got there she was undelotted and then they went to an oral morphine tablet and then they went to a liquid morphine you know with a dropper dropper yeah but all of those are opiates right and so all of them have the effect of clogging up the digestive tract which meant she couldn't eat well and and it was she had a really hard time keeping food down when she did eat um so yeah it was it was so you were there for until I was there a month a month yeah the last month of the life the last month of her life and so you you got to visit and talk and oh yeah spend and then of course family and and our husband's family is huge and he's from Utah no he's they're actually both from southern california but his his previous generation was from utah so his his father and his uncles and and grandfather them they're from a place called greenville which is in south of Utah and and that's and but they but where and that and danny were living was north and east of salt lake yeah well we are going to take a break sure and when we come back I want you to if you don't mind telling us about those last few sure okay yeah hello ha my name is josh green I serve a senator from the big island on the conus side and I'm also an emergency room physician my program here on think tech is called health care in hawaii I'll have guests that should be interesting to you twice a month we'll talk about issues that range from mental health care to drug addiction to our health care system and any challenges that we face here in hawaii we hope you'll join us again thanks for supporting think tech hello my name is crystal let me tell you my talk show I'm all about health it's healthy to talk about sex it's healthy to talk about things that people don't talk about it's healthy to discuss things that you think are unhealthy because you need to talk about it so I welcome you to watch quok talk and engage in some provocative discussions on things that do relate to healthy issues and have a well balanced attitude in life join me I'm bill sharp your host for asian review a weekly um show right here on think tech hawaii that's devoted to substantive analytical discussion about contemporary events in asia by asia we mean anything from hawaii west of pakistan and from the russian fiery south to austria and new zealand hi we're back and this is my dear friend walter peters and walter has been telling us about the horrors that his sister went through living in utah which is basically a Mormon town state and without the choices and options that we here in hawaii are are working hard to see that our people do not have to go through what his sister went through which is why i asked walter to talk about it because from for so many of us it's just this piece of paper that says this is a bill and then somehow we get to the end and say oops this is what this was about right so walter again tell me now your sister was 45 44 years old 44 yeah and she had she had terminal cancer she actually had three cancers three different cancers so here's what it is the breast cancer even though they successfully treated it it actually is never gone and yeah i know it's still in the body yeah right you know she had that she had bone cancer which was very active and aggressive and the third one i'm trying to remember oh yes it was on her lungs and what it was is she'd had so many um x-ray workouts over the period of time she was in intensive treatment that the x-rays had actually caused lesions on her lungs which had made it really hard for her to breathe and to to sleep well and it had it had metastasized the bone cancer somehow had affected the lungs to where she now had a lung cancer going on so yeah so she had three three cancers and any one of them would have taken her out eventually it's just hard to to imagine yeah what what she went through so now you you stayed with her for a month right now tell us about this last part of the month of the of hers being she had hospice she had hospice yeah and but it's not like what we know here where they they're there every day because we have so many yeah actually you know it's a funny thing the the Mormon community right they have so many different service organizations within their community structure that she had people coming by the house these are not you know medical professionals they're just folks who cared right they'd bring food and they'd do a massage and they'd help her whatever it was they cleaned the house you know I mean without them it would have been a lot worse because her husband you know wouldn't been able to go work right right and that's one of the things one of the benefits of me being there for him was he could go work and I could help take care of her so yeah it was a community process for sure now even though she had all these drugs was it a peaceful passing was it no no I mean it was she was tremendously uncomfortable part of it is that you know bone cancer it's it it it just hurts being there yeah right and plus she'd had a break in her bone you know in august and here we are in um early october and nothing's been done about the break in the bone right so she can't there's no position she could get into where she had zero pain without medication so she was constantly on some medication and you know we had all these different uh apparatus you know a chair that moves and whatnot to try to hold her in a position that's the least painful and all that but the bottom line is that she was always have always medicated and the medication had impacts on her besides the one that was desired and she was just suffering I mean I I cannot count the number of times that she said to me how long is it gonna take me to die because the doctors thought that she'd already be dead right um she had been told at least three or four times that you've got three to six months to live right yeah that was three years ago oh my god so it's just like how long is this gonna take how long do I have to go through this how long do I have to do this suffering thing so yeah it just got to the point where it didn't make any sense to to prolong it if there was any way to make it happen sooner that was the thing to do yeah well you know I think I need to make it clear to our audience that in this bill in the bill that's before the legislature now it talks about the fact that the patient not the doctor not the family the patient like your sister right can say the cancer has eaten me up yeah I am not taking my life Mike the cancer is taking my life yeah it was already like there was no questions about whether she was gonna live or not the only difference is how long is it gonna take yeah and why should she have to suffer like that right why should her husband have to suffer why should you have to suffer like that yeah I mean that's just inhumane to make a person do that right so now once she's in this did they do terminal sedation that's when they well there is I don't know that there's an option there's an option for that and you know we have a mutual friend that's a retired nurse and of course I was on the phone with her a lot you know about what could we do and what not and she was making recommendations but again the hospice situation right the time delay between having an idea and a decision and a request and having to actually get implemented that was unworkable um so we it just turned out that um that that might have been an option and that's not how things turned out we actually got into communication with an organization that's a national organization that um that assists people at the end of life and um and they explain to us their process and what they do and uh and but they have all these protocols that they have to go through to protect themselves oh yes because there's going to be somebody disgruntled about it yes right so they have to they they have to go through a whole bunch of uh steps to make sure that they remain anonymous and and uh fortunately we didn't have to go through all that um but yeah so it just but but they showed us how we could support her so that we could support her to get complete good because uh just to explain to our audience that as the law stands now in Hawaii if a doctor uh prescribes something so so that the patient can in fact right end the suffering uh if there's a disgruntled person the doctor might be charged with manslaughter right so there's all the bill protects the doctor right and you know to keep it's just hard to believe that somebody would be charged with manslaughter when their intention is to assist this person to end the suffering and in the bill the doctor does not touch the patient right the patient has to ingest it themselves they have to do this right um so i'm back to your sister so so tell me did she finally did you how did how was the end well did i'm gonna walk i'm gonna walk discreetly okay and um she had assistance yes and her end was peaceful oh good and um her husband and i were there with her and um and the only thing i can say is that everybody was relieved that it was finally over you know there wasn't anybody going oh too bad no it was everybody was like thank you okay now what do we do we take care of what there is to take care of but um but yeah it was it was challenging um to just be in that space just be there with her as she's going through so much struggle and so much discomfort and and you can just see the agony that she's going through i mean i don't i'm not even gonna get into the things that i needed to do for her to be able to breathe it was just not i never want to see a thing like that again in my life but that's what it took right but that was just so that she could get air not any you know comforts just to be able to breathe i had to go through all sorts of things just to be able to clear her airway was it was hard so so to be able to you know to know that she was uh find me a piece was just the biggest relief yeah oh yeah i asked walter to come so that we could hear first hand this sort of thing what it does not only to the patient but to the family because we want this bill to pass so that the people what's her name annette so that that there aren't a lot of other annettes yeah we have to have it passed so that people well you know here's the thing this is what's so had there been legislation like what you're talking about in place there and then her suffering could have been diminished tremendously and her actual end process could have been much more comfortable for her and you and us and us absolutely could have been but uh you know that's just that's not the case yeah you know and you've got people who um basically what what the law currently says is that doesn't belong to you we will tell you when you can go you will tell you when it's okay versus that's yours do with it what you will what amazes me is that if you're in the hospital at ten thousand dollars a day they can turn off the ventilator which seems terribly cruel yes or you can decide not to have food and water that that's just all of that seems cruel yeah and or they can do terminal sedation which is they give you some kind of a drug right and then they keep drugging you till you right well you're unconscious and you go right which again seems if all of that is legal then why is it not legal to allow a person to choose give them the doctor just writes the prescription he doesn't touch so if your sister had that and and she said it would give her comfort to know right that if i want this if this becomes too hard i have something right and in the bill you have a right of refusal right if you order it and then you say i think i changed my mind you don't have to take it right and it just seems so humane and listening about your sister i just hope and pray that we enough people will write about annette in the name of annette and say we've got to pass this we can't have another annette yeah well the reality is that there's so many there are so many and you know the way things occur right now it's just not my experience i mean i'm not an expert but it's not my experience that the patient is the highest priority i just know how it looks to me when i see the system and what she went through you know it just it doesn't doesn't show up to me like that her well-being comfort and peace were the highest priority in the system it didn't show up to me like that and you are correct yeah and g walter it's been a pleasure spending this time with you and i can't help but cry thank you thank you so much i hope we have an impact


Hello, the subject of this video is Swiss Orthology, a tool and a website which combines Swiss Institute of Bioinformatics Orthology resources. It aims to provide orthologs inferred by both resources and to guide you in choosing the most appropriate for your analysis. Imagine the following scenarios. Your molecular biologists studying a particular gene in the laboratory. You mainly do wet lab experiments, but you want to know more about your protein and its evolutionary history. Where do you start? Or you're a bioinformatics PhD student working in a lab that just sequenced a rare species. You've been tasked with the annotation and functional prediction of its proteins. Where do you begin? When faced with these scenarios, generally the following thoughts might go through your mind. What's the difference between an orthologue and a paralogue again? Will I have to download, learn and run some software? How painful will that be? What's the fastest way I can get orthologs between my species of interest? And can I understand the basics of what's going on in the black box of this software? Enough to know if it's appropriate for my analysis, its limitations and enough of the method to describe it. One way to help solve these problems is Swiss Orthology. Swiss Orthology is a recent SIB funded initiative aimed at serving the orthology community, both users and method developers. OMA, which stands for Orthologist Matrix, and OrthoDB are two leading resources which provide pre-computed gene orthology and standalone software for custom orthology analysis. Swiss Orthology is a joint collaboration between both groups, but Swiss Orthology is more than just OMA and OrthoDB. It supports services such as BUSCA and the Quest for Orthologs benchmark service, as well as community activities such as the yearly philosyve meeting for the Swiss phylogenetic community. But before going more into what Swiss Orthology offers, it's necessary to answer that nagging little question. What exactly is orthology? We must first start from the beginning. Evolution is one of the fundamental principles of biology. A major concept in evolution is that of homology or the relationship between genes related by a common ancestry. From this general homologous relationship, pairs of genes might be classified into any various subgroups of homologs, including orthologue, paralogue, zanologue, or homeologue, among others. There are two main types of homologs we will discuss, orthologs and paralogs. In comparative genomics and phylogenetics, the fundamental concept of orthology relates corresponding genes in different species. Orthologs are pairs of genes which have evolved from the same gene in the last common ancestor. Thus, orthologs started diverging due to aspeciation events. In contrast, paralogs are pairs of genes which started diverging from a duplication event. All of this can be visualized on a gene tree. In this example, imagine there was a common ancestral gene A. A duplication event produces paralogous genes A and B. Later, aspeciation event occurs, which causes the A and B genes to further start diverging into species 1 and species 2. Thus, these definitions are based solely on the evolutionary event which gave rise to the pair of genes in question, either aspeciation event, orthologs, or a duplication event, paralogs. Sometimes it is useful to deal with groups of genes which are derived from a single gene in an ancestral species. This is what we refer to as hierarchical orthologous groups or hogs. Hogs are clusters of orthologs and paralogs defined at a given taxonomic level of interest. Here in this example gene tree we have three hogs nested and defined at particular taxonomic levels. What advantage do hogs have? Well, they relate genes from multiple species and also give a finer level of granularity depending on your specific taxonomic level of interest. A hog is another way of looking at a gene family. For more information on hogs, see our YouTube video, What Are Hogs? Orthologs are useful to a variety of applications in genetics, genomics, cell and molecular biology, and of course evolutionary biology. Among the many applications include prediction of gene function, verification of function conservation, phylogenetics and phylogenomics, finding the best model systems to study a gene of interest, phylogenetic profiling and elucidating a gene family's evolutionary history in terms of gene birth, death and duplication, among others. A wide range of methods have been developed to infer orthologs, including OMA and orthodepine. There is a lot more on orthology. If you're interested here are some starting resources. The links are provided in the description. There are many types of homologs and many methods to infer them. One thing that might be difficult to know when starting your analysis is what type of orthologue would be the most appropriate type for your study. There are many orthology databases out there. With a focus on OMA and orthodepine, we aim to provide guidance to help prospective users determine which platform is suited to their needs. For this, we provide the Swiss Orthology Guide. Here you can interactively find information to help you choose between different Swiss orthology tools. For some applications, either OMA or orthodv is better suited. For others, both orthology resources are fine. In the flow chart, you can interactively decide which resource to use based on the type of species, type of analysis or format of data. Let's take one of the examples from before. You're the molecular biologist wanting to study the evolutionary history of your gene by making a phylogenetic tree. Navigate to Swissorthology.ch. You can then follow the flow chart by choosing Guide in the upper right-hand corner, type of analysis, then build a gene tree. We can see that both OMA and orthodb have functions in their browser to help get the orthologs of a gene family and to build a tree. Links to the respective website and more information are provided. The main service that Swissorthology provides is actually a tool to find and download the orthologs of your favorite gene from both OMA and orthodb at the same time. This is made possible by storing the data in RDF format and querying both SIV databases using Sparkle in the back end. This way, with one simple search, one can get the hogs from either OMA, orthodb, or take the intersection of orthologs or the union. Back to the scenario where you're trying to make a phylogenetic tree of your favorite gene. In order to get the orthologs between the two species, predicted by both OMA and orthodb, first search the Unipro KB identifier, which will take you to a table where the rows are different taxonomic levels, and the columns show which resource detects and or computes orthologs at that level. In this example, we can see that both OMA and orthodb computed hogs at the embryo phyto level. We can click on this row to be taken to the set of genes inferred to be orthologs to our gene of interest. Each row is a protein from the species at the embryo phyto level or below. We can see whether the proteins were predicted to be orthologs by OMA or orthodb resource. One can then download the sequences in faster format from one or the other resource both or the intersection. In this example, some genes are predicted in either OMA or orthodb simply due to differences in genomes which each database uses. For example, orthodb inferred orthologs using data vulgaris while OMA does not. More information about which species are used in each resource can be found in the Swiss orthology guide. In summary, Swiss orthology is an SIB funded resource which supports the maintenance and development of both OMA and orthodb to leading methods for inferring orthologs. It provides orthology guidance to know the difference and to help choose the best resource for your particular analysis. It also allows users to query both resources simultaneously and download the orthologs. As Swiss orthology is still in development, we want to hear from you. Any feedback, comments or suggestions are welcome at info at Swissorthology.ch. Many thanks to the Swiss orthology team and to the Swiss Institute of Bioinformatics.


I'm feeling so good here. We go. Oh my god. I'm still spinning bro. Oh Snap. Oh bro. Let's go Lincoln bio Where's the button I can't why is it not?


Hi everyone, Joe for JaspiesCaseBrace.com coming at you with 2018, Panini certified football. Four box, random team break number one, popping from a fresh case right here. All card ship, all teams are in, big thanks to this group for knocking this out straight up. No fillers here, appreciate it. All 32 are in, let's go, let's roll it, randomized names and teams, one and a five. Six times for names and teams, one and sixth and final time, down to Gale, one and a five, six times for the teams, good luck, one, two, three and sixth and final time. After six, we got the Eagles down to the Colts, Bennett with the Eagles, Patrick with the Packers, Logan with the Titans, Kevin with the Steelers, Mack with the Chiefs, Gale with the Saints, Blake with the Patriots, Charles with the Ravens, Ryan with the Broncos, Mike with the Rams, Bennett with the Bears, Gale with the Cardinals, Kevin with the Giants and Dolphins, Patrick with the Raiders, Charles with the Seahawks, Chris with the Texans, Kevin with the Falcons, Chris with the Bengals, Kevin with the Cowboys, Patrick with the Niners, Simon with the Lions, Michael with the Bills, Aaron with the Panthers, Michael with the Buccaneers, Kevin with the Jets Bennett with the Jags Scott with the Vikings Patrick with Washington Karen with the Chargers Michael with the Browns and Gail with the Indianapolis Colts and remember trade at your own risk remember this is the 2018 draft class 2018 NFL draft let's Google that really quick that takes me to the Wikipedia page this is your Saquon Barkley quarterback Josh Allen rookie year so on and so forth mink of Fitzpatrick Lamar Jackson Nick Chubb all the Darius Leonard etc etc etc all right so let's flip over here well I'll flip back to the team list in a second see which boxes we're gonna do here so there's three columns of four and we're gonna use we're gonna select one die I'm gonna select select that one right there and we'll go one two three four five six on the dice roll and it's one so we're gonna do the far left side and we'll save these for next time right so those are the boxes let's flip back to the team list really quick when we come back we're gonna see if there's any trades and then we'll have the four box break stick around BRB welcome back no deals were done so the list you saw on the previous screen remains the same all right so let's see what we got 2018 certified football for autographs or memorabilia cards per box on average I mean yeah Rex you're listing some all-time greats right there you're saying it's crazy how no pitchers ought to be we're able to become Nolan Ryan Bob Gibson Tom Siever Bob Feller I mean those are those are also all-time greats I know what you're trying to say though I mean just in general yeah you're seeing seeing lower pitch counts people aren't going going too deep but into games but I mean a little a little unfair to wonder why there's not gonna be another Nolan Ryan or some other Hall of Famer Hall of Famers are rare it's hard that's Mike Gasicki need more penny sleeves here Kevin Benedict with the Dolphins but yeah I think the I think the era of guys throwing 130 pitches and getting complete games I mean that's been that's been fading away for probably the last 20 years I guess maybe even more so now but there's a 101 out of 399 Anthony Miller for the Bears and it'll be for Bennett and Chicago Pablo Lamar did I did we see a Lamar good I Brett just breezing by rookie Lamar Jackson that goes to Charles and Baltimore thanks Brett is Antonio Calloway Jay you can look up our videos and look for look for flawless that'll give you an idea of what that's all about kind of hard to explain in the middle of a break here there's Andy Dalton certified gamers 49 out of 50 so for Chris and Cincinnati Duke Dawson to 999 out of 499 work done Chris Carter to 999 Josh Adams to 99 second-year homes DJ more rookie roll call 15 out of 35 nice nice Kevin congrats Cameron break to 499 box one what break are you guaranteed to hit something there's no break well unless it's a random hit break but most random team breaks you're not guaranteed anything if you want to get be guaranteed something you should check out our Instagram stream at Jasper's breaks on Instagram live you can buy a personal box of something and everything inside there would be yours all the hits included you be guaranteed something then but most group breaks you're not guaranteed anything unless it specifically says you're guaranteed something but those kind of those stat style of break is a little rare it's Colton Miller to 499 for the Raiders and we've got Aaron Rodgers and clutch performers Russell Wilson 61 out of 199 where does uh where does Russ end up or does he say we see out that'll go to Charles Luke fall to 999 Phil Rivers to 225 Ranny Moss 999 Aaron Rodgers we've got new generation Neum Heinz 173 out of 399 Colts scale Colts I think he should be doing a March Madness promo of some kind I don't know if it'll be a bracket promo but I'm sure there'll be some kind of promo or maybe not I don't know you know you're gonna have to do Rex check the website every day every minute Jasper's case breaks comm never miss a moment just check the website every single day every single hour every single minute 24 seven all the time there's Jared McKinnon for the Niners mirror signatures 19 out of 50 that'll be for San Francisco that's gonna be for Patrick Quentin Nelson to 999 and a redemption there's Jimmy G are these expired 2018 probably yeah at this point they probably are but we'll still send it to the team that this goes to freshman fabric mirror signatures we got Hayden Hurst Hayden Hurst was a Raven back in 2018 right is he still Raven this expired in 2020 they may it may still yeah he was a Raven it may still have that I don't know well you can check with them Charles all right next box got rocky blear we got out of 999 Dylan Contreras and an Alvin Camara clutch performers relic 197 out of 399 that'll be for Gail and the Saints got Randy Moss to 499 Cortland Sutton new generation relic for Denver I'm gonna go to Ryan refer hands off the glass KG Warren Sapt 99 Carlos Hyde Bradley Chubb to 50 JJ Watt that's to 225 Mark Andrews great iron signatures 35 out of 75 for the Raven Charles maybe we can find his teammate somewhere Dylan Contreras again for the Chargers Denzel Ward to 999 and Neum Heinz two-color patch Nato to 16 out of 349 the Colts looking for a new quarterback all right final box all right clutch performers Devante Adams to 399 where does he end up free agent right chase Kenan 499 Richie James to 999 got Josh Allen relic new generation relic Buffalo Michael with the bills Carson Wentz to 225 I should get a new what's a neuro link I don't think I want that doesn't sound like something I want there's Richie James to 499 and that's Isaiah Wynn to 50 their car Sam Hubbard certified potential autograph 29 out of 75 for Cincinnati Chris with the Bengals to Terrell Suggs to 499 got Jalen Samuels freshman fabric two-color patch Nato 85 out of 349 that's for Kevin and Pittsburgh Elon Elon Musk wants to put chips in people's heads and link them to a mainframe sounds like the Matrix to me doesn't nothing I'll pass on it sounds cool though if you're into that sort of thing all right that's that ladies and gentlemen I'm Joe for jasper case breaks calm that was certified one we've got another four box break in the source it's a pretty quick break only a 20 minute break still not seeing the big names yet from this 2018 draft class you know I'm talking about so maybe the next four box so we can run that back and maybe find some more of that guy or Josh Allen or something like that there you go gang I'm Joe for jaspercasebreakstock.com 2018 Panini certified football in the books I'll see you next time for the next football break


Studies suggest that the stromalin antigen, SA, protein is essential for cohesin's proper functioning and loading onto chromosomes. It has been shown that SA proteins remain bound to chromatin when IED21, a core component of cohesin, is absent from cells. This suggests that SA proteins may be responsible for cohesin loading onto chromatin independently of IED21. Furthermore, SA proteins interact with INA and R-loops, suggesting that they may be involved in regulating gene expression through INA-mediated mechanisms. Given that SA proteins are highly expressed in many cancers, it is likely that they play a critical role in cancer development and progression. This article was authored by Hailey Porter, Young Lee, Maria Victoria Nagemba, and others.


This one was a shocker. Oh my goodness got it at a garage sale for a dollar I think it was sitting in my money pile also. Hey fellow buddies. Thanks for watching Amazing, right? All right, let's get started Hey fellow buddies. Thanks for watching. All right in this video. I'm gonna talk to you about my big money Bolo's I'm gonna tell you where I got it what I paid for it and what it sold what it sold for on eBay These items I picked up pretty cheap at thrift stores the state sales garage sales you name it all source anywhere YouTube auctions I get mystery boxes from thrift stores. So we're gonna go over and Check out some screen sharing of some big money Bolo's If you're new to the channel, let me know how you found it and If you're not new let me know how you found it Tell me how you found the channel and if you have not subscribed I would love for you to subscribe. All right. This one is just kind of like Awesomeness I got this at an estate sale. It was an estate clean out and I have been getting stuff there all summer long These are by June Taylor. They're mix and match templates I thought about parting these out and then I just decided I'm just gonna put them all together to save some time and They're quilting templates. I sold these for a best offer of $46 plus shipping These sold really quickly actually and I got them Again, I was buying in bulk. So my cost of goods was really low These are scratch and sniff stickers and I picked these up at an anonymous place You're gonna see this in a video, but I want to save it for the video But my cost of goods was next to nothing. Okay, I sold these for $64.99 plus shipping. These are the scratch and sniff Hotdog stickers six of them here you guys $64.99 a buyer was all in for $74.72 for these scratch and sniff stickers So stay tuned for a video that's gonna tell you where I got those and Feel free to guess in the comments. All right, I'm wondering if on this one. I undersold it and I Had it up for auction and somebody When I do auctions a lot of times I will put or best offer Somebody sent me an offer of $80 and I went ahead and took it. They sent me lower offers first But when I do an offer on an auction It has to be higher than what my asking price is my starting bid Like I'm not gonna entertain a $50 offer on an $80 start price. Okay? But if somebody offers me a hundred I might it just depends this is a great way to feel out the market But it's also very frustrating because sometimes you're like should I take it? Should I not take it? But anyway, I got these at that same estate sale So my cost of goods was very low and somebody came in and offered me $80 and I went ahead and took it I did my research, but you know, I couldn't find any complete sets So it was kind of hard to determine whether or not I was in the right price range or not Should I have let the auction ride? Maybe let me know what you guys would have done in the comments buyer was all in for 9918 and they did leave a they either messaged me or left it in the feedback that they were super excited to Find a complete set. So I think that's what made this so rare. They are brass house numbers. You guys check them out So let me know if you think I undersold it The next item are these Starbucks Christmas family barista bears a lot of four I got these out of a thrift store mystery box and I took a best offer of $38 plus shipping on those The next item is this Elsa Williams jeweled pillow cruel embroidery kit. This was still I'm sorry. Item is new old stock sealed but seal is broken. So if you look back here I marked this pre-owned because of that just in case something fell out. I don't think anything did but to me It's new old stock, but man, it's just not perfect. You know what I'm saying So I went ahead and put this up for auction sort of for $89 and 90 99 cents plus shipping. This also came from that same estate clean out These are Sigma jigs. They are Fisher price Singing plush and I tell you guys all the time pick these up if you see them they sell I got these at the Goodwill Benz. They were all sitting together I do have footage in one of my Goodwill Benz videos and I Sold these for $60 plus shipping. So my cost of goods. It's a dollar 89 a pound I'm guessing maybe three or four bucks for these and I decided to sell them together because there were a lot listed Individually and I figured that would kind of give me more control of the market as a unique different item That would stand out if I sold them as a lot. So that's why I did that These are vintage fontini depots Italy wise men from the nativity This is what they look like on the bottom and this is what they say and you can see they are hollow I ended up selling these for a best offer of $37 plus shipping I believe I got these at a garage sale, but I don't remember what I paid for them This is an alphabet sampler kit. This also came from that same estate I ended up selling this for $46 and 50 cents plus shipping any time you guys can find these sealed kits They do range from big money to bread and butter It really just depends but my cost of goods. I bought them in bulk from the estate buyout. So I Bought every single one they had because even if it's bread and butter I was still gonna make money, but I have actually found that some of them are doing really well a lot of them were samplers This is the San Francisco music box snow globe. I'll be home for Christmas I got this out of a thrift store mystery box and I sold this for $62 plus shipping This here I'll tell you what I thought this was gonna fly out of my store because it was so different and unique It did have a little bit of issues, but it's just a handmade item I got this at a thrift store for three bucks and it took a couple years to sell if I remember correctly Maybe it was only a year But it is a one-of-a-kind primitive grungy pumpkin head jack-o-lantern doll. It's a folk art item Paid $3 for it. I ended up taking a best offer of $55 plus shipping. I really thought it was gonna go for more than that I thought it was super special. I mean Look at the little outfit it has on. Oh my goodness. It's just incredible It's incredible, but you know, it was listed for so long and maybe I didn't have the best keywords I don't know but 55 bucks. I'm still very happy with that but thought it was gonna go for more This is a 1992 puff-a-lump Fisher price yellow duck got this at a garage sale for 25 cents I went to a garage sale over the summer and all of their plush was 25 cents I left there with so much plush. I have a whole video on it It says something like plush. I got for a quarter 25 cents or something like that You should definitely go watch it because oh my goodness the plush I got for a quarter Unbelievable this guy sold for $42 and 25 cents plus shipping. He did have a little defect there on his foot and the buyer paid shipping This is a Hasbro for real friends cuddles my giggly monkey interactive pet from 2012 I got this at a garage sale for $2 and I sold it for a best offer of $36 plus shipping I did put a video in my description and that is over on my reseller testing Bolo products If you guys want to see some of the things I'm listing and how they work you can check out that channel It's linked in the description Calico critters these are I believe these are retired what they are retired I believe their vintage also But these are the puppy dogs and I got these at an estate sale actually in a big bag of a bunch of calico critters And I paid one price for all of it I don't remember what it was but my cost of goods is probably like a quarter for these because there were so many in there And the price was just excellent. I sold these for $42 and 25 cents plus shipping These vintage brass dough and buck deer reindeer. I got these at a thrift store for $1 I sold these for $35 and 75 cents plus shipping This here is a kelly toy big stuffed animal monkey jumbo huge plush This was a viewer reached out to me and they are local to me and they said that they had a bunch of plush and They needed it gone by the next day. Could I come pick it up? That they didn't want just anyone coming they trusted me and they just needed it gone and they would give it to me So um, it kind of put me on the spot because you guys know how much inventory I have and I'm like, oh my goodness I do not need more stuff, but I'm like, how do I pass up on amazing plush? I love selling plush So I went and picked it all up And you're gonna see I think you've seen some of those unboxings On some of my reseller vlogs already. There's gonna be some more coming But this is one of the items I got. How cute is this monkey? So this monkey sold for $38 best offer and the buyer paid shipping. So this was free to me from a viewer Thank you so much. I appreciate you Really really kind so it helped me and it helped her and it was just a win-win The next item is this vintage stuffed santa claus figure christmas decor hand-painted face folk art. Look at this It is incredible I got this out of a thrift store mystery box and the funny thing is I had this in my garage sale And I pulled it from my garage sale and I listed it on mercari mercari only that's what I was doing that day I was like, I'll just put this on mercari real quick somehow it got deleted from mercari And I was like, I need to put this on ebay because I have since then realized that items that are folk art Santas do really really good whether they're wood or plush or whatever be on the lookout, okay? And I put this on ebay and it sold super quick for $55 the buyer was all in for 66 93 on that All right. I did a video on these. These are the pixel chicks house. It is an electronic game. These are incredible um I think these either came out of a mystery box or I got them at a garage sale I don't even remember they've been sitting in my garage forever I sold this one for $59.99 on auction and the buyer paid shipping definitely be on the lookout These here came from a thrift store. They were sitting outside in the free boxes. So This thrift store that I go to if the items don't sell Because it's such a small store they put the stuff outside and it's up for grabs for anybody that comes early They can just take whatever they want now If I take something from those boxes, I give them extra money when I check out Just because I feel like I'm a reseller. I'm not taking this for personal use.  So, um, That's what I did So I don't remember how much extra I gave them But I ended up taking a best offer of $53.80 for this and the buyer paid shipping And they are the vintage japan monkey mug set here. No evil see no evil speak no evil The next item is this an of green gables pop up dollhouse Book it is sealed new old stock. This came from an estate sale Cost of goods was probably a buck or two sold it for $46 plus shipping. This also sold very quickly This one was a shocker. Oh my goodness got it at a garage sale for a dollar I think it was sitting in my money pile also It's a bill to bear white elephant plush. It's root off the red nose reindeer It's I think one of the misfits, but it's only nine inches big you guys. Oh my goodness. Look Here's the bill to bear tag. This is what you're looking for I sold this for $77.50 plus shipping and this sold super fast This next item is a vintage brown american tortoise Taurus tortoise. Did I spell that wrong? Oh my gosh. No tortoise stir. Taurus stir Taurus stir, okay, there we go. It's a travel bag. This came from that estate sale There were some suitcases sitting there and I'm like, what are you doing with the suitcases? She's like you can buy them. I'm like, I think I paid a buck each for him So I sold this for uh, $40 best offer and the buyer paid shipping Here's another advent calendar. This one did not have the mouse no mouse with condition issues Soda for $93 plus shipping uh, the buyer was all in for $112.32 on that and I got that at the Goodwill Benz This one is a hand carved wooden nativities This came out of a thrift store mystery box It did have like the little building with it But I ended up donating the building because it was just too much to ship And I parted this out and sold these pieces all together for a best offer of $85 plus shipping All right, you guys What did you think about those? Bolos which one was your favorite are these items that you would have picked up or would you walk would have Or would you have walked right past them? Let me know down in the comments Thank you for being here be sure to like comment subscribe And if you would share this video out that would be incredible. Um, thanks for watching


Tuminelli mr.


There's a saying that a picture is worth a thousand words now since this video is running by at 24 pictures per second That means you've just seen a novel Although since it's been the same picture the novels are pretty boring one But I digress let's talk about the mathematical equivalent of a picture the graph of a line Now remember that any point in the plane can be specified by its Coordinance x and y a graph consists of a set of coordinates For example, I might try to sketch the graph of the following set of coordinates 1 3 1 5 and 2 4 So this first set of coordinates 1 3 Specifies a point that has x coordinate 1 and y coordinate 3 So we'll plot that point, but we'll also label its coordinates a good habit to get into Next I'll sketch the point with x coordinate 1 y coordinate 5 and Finally the point with x coordinate 2 y coordinate 4 Now it's important to understand that because our graph is given as these three points only Then our graph consists of these three points only Sometimes we have too many points to list them conveniently and so instead we represent the graph by writing the Relation between the x and y values For example, I might say something like this Suppose the y values of a point on a graph are three times the x values First of all we want to express this as an equation and then find three points on the graph Since the y values are three times the x values Then whatever the x value is the y value will be three times that amount and we can express this as Y equals 3x Now to find three points on the graph what I want to do is I want to find three sets of x and y values Now I have this relationship y equals 3x since this is going to be true for any x value We can choose any x value that we want So let's choose How about x equals zero as an easy value to work with if x equals zero Then our formula tells us that y is equal to Zero and so the point zero zero is on the graph Well, let's pick another value for x. How about Let's choose x equals one If x equals one our formula tells us that y is equal to Three so the point one three is also on the graph And if I choose x equal to two our formula tells us that y is equal to six And so we know the point two six is on the graph Now while we know these three points are on the graph We don't know if there are any others on the graph and we could find more by choosing different values of x And if I were endlessly patient I could fill in the space between these points with many many many more points But for reasons that won't be clear until you take calculus We can connect the dots to form a continuous graph Mathematicians say that the equation y equals three x corresponds to the curve shown And it's important to recognize two things First mathematicians are not very good at coming up with new and exciting names for things This is a curve and there's no real need to call it anything else and when a mathematician talks about curves They include things like this straight line So for example, let's draw the curve corresponding to the equation y equals five So again the equation describes a relationship between the x and y values on the curve As before we can pick a value for x and find the value for y So I'll pick x equals three because I like the number three then our formula tells us that y equals five So three five is a point on the graph I'll pick another value for x if x equals ten then our formula tells us that y equals Five so ten five is a point on the graph and if y equals well Why not let's use this horrible mess then our formula tells us that y equals Five and so I have a point on the graph after plotting these points I can connect the dots and carefully label what I've done Which gives me the graph of the equation y equals five Now let's pause a moment and think about this process if we had y equals any other number We'd still have the same value of y regardless of the x value And so we'd still get a graph that looks very much like this and this leads to the following theorem The graph of y equals C where C is any constant is a horizontal line Let's try another one. How about let's try and graph x equals three So as before we'll try and find pairs of x and y values So if x equals two then our formula says x equals three but that's impossible because if x equals two x can't be three and What this says is we can't pick values for x So we should Pick values for y instead So if y equals one then formula says x is equal to three And so we know that the point three one is on the graph If y equals five then x equals Three so three five is a point on the graph and if y equals eight our formula says that x equals three So three eight is another point on the graph And if we connect the dots we'll get our continuous curve and again if x is equal to any other number we're going to get a very similar graph and So this leads to the result the graph of x equal to C where C is any constant is a vertical line And let's pause a moment and see what we've gotten if y equals C is a horizontal line and x equals C Is a vertical line then what happens when we have an equation that involves both x and y like 5x plus 3y equals 15 as before we'll find some points But before we proceed we'll throw down a useful definition and a theorem The standard equation of a line is ax plus b y equals C Where a b and C are real numbers Well, actually I'm putting Descartes before the course Because we should actually point out that the graph of ax plus by equals C is a straight line So let's take a look at the graph of 3x plus 5y equals 15 So our definition says that we have the standard equation for a line and The theorem says that when we graph this we will actually get a straight line So from geometry we know that we only need two points to graph a line So let's find two points that satisfy this equation Since we get to pick our x or y values We want to pick the hardest possible value so that people will marvel at our awesomeness Or we might actually want to pick the easiest values that we can work with So we like zero if x equals zero Then substituting that into our formula gives us an equation that we can solve for y and we find Y equals five so zero five is a point on the graph While we could pick a different value for x and solve for y We might find it a little bit easier to let y equals zero So we'll let y equals zero and substitute that value into our equation and then solve for x And if we do that we find that three zero is also a point on the graph And because we know that this graph will produce a straight line We'll draw the straight line running between these two points


We're live here at Binway Park Bell Time Club Dante Luna. I'm here with Liza Rios the wife of the lyrical legend big pun Dead in the middle of little Italy little that we know that we riddled to middlemen who didn't do diddly Absolutely welcome the Bostonizer. Thank you. Thank you for having me. I appreciate it. Oh Absolutely, we have Kongo versus Jeff versus Mr. Briggs No, they're not Josh Briggs rather and Liza why are you here? You win big pun with big wrestling fans. Yeah And if I can recall correctly you guys were even at WrestleMania 15 But did a great performance it was facing everybody the kids Josh Briggs on the attack big Kongo Man, these guys are heavyweights. Look at that. I got my buddy on the big one. Yeah To contrast that I'll take mr. Kongo Can you talk about this environment here at Fenway, how have you been enjoying yourself this weekend? Oh, man, it's been awesome coming into Boston. Yeah, I'm saying we was at Lace yesterday Now we're here at Fenway Park first time being out here and it's a great vibe I'm safe loving the action Absolutely Absolutely, and I hear the New York accent from the Bronx. Yes, I can't get away from it You already know it. Oh, it's not taking out the Bronx, but can't take the bronze out of it. Absolutely Looks like your pick. Mr. Briggs here is getting dominated right now. Oh, man Kongo with the headbutt. I don't want to lose Briggs. Come on You got to put him in the figure four and break that ankle like punk was saying absolutely Haha Could you tell us some more stories about? Good children because we know star and Chris they both have EPs out. Oh, yeah, definitely I mean, um, Chris Rivers, he just dropped the album called the DeLorean So that's for now. It's definitely it's one of his best projects out So he should be touring around soon that star real just performed the first day for the first time the other night We're on the relief of the DeLorean. So she's dropping the EP soon She's out here, you know representing You know herself and on the family and selling some amazing shades out there. So yeah, I'm very proud of them They are they definitely have a bright future. Absolutely. It's definitely honor to have you here with us This action in the ring is incredible right now your pick the man who you have the money on Pressing the offense Pressing the offense is making you look well To the face Off the ropes, oh boot to the face right now Congo needs the kind of eat that shoot absolutely ate all size 15 of that boot Oh He hasn't lost yet This is not good The big choke slam for the cow one three Ladies gentlemen, Liza Rios the betting woman. You are the one a million dollars right now You know how to pick them.  Josh Briggs with the victory Absolutely, he definitely all went and thank you for joining us big time bell time club Dante Luna finlay park donny wonderful Liza Rios


So how long has been Collabora involved in the LibreOffice project? Well, that's a really good question. Of course, Collabora has been around for eight years now doing great things on LibreOffice, but we've founded LibreOffice together, as you know, at Arlo. So I think we've been involved for a long time, and many of my team came from SUSE, and were spun out and helped form Collabora to help sustain and drive LibreOffice. Can I have a productivity, I should mention? Yeah. Which are Collabora main objectives for sponsoring the LibreOffice conference? Yeah, so great question. I mean, I think really, I often go just to meet the people and understand what's going on. You know, of course, to celebrate another year of achievement, I think is always good. And so many friends and colleagues at the conference, and there's some great content there, I think, too. And, you know, inspiring technical talks, you know, exchanging ideas and sharing information is really pretty cool. And the hope is, of course, live streaming, and then we can reach even more people when we can actually, you know, gather in one place. So yeah, and we just love to, you know, show off the value of the, you know, supported versions of LibreOffice, particularly, you know, Collabora, CollaboraOffice and, you know, the value then for people wanting to migrate with actual real support from, you know, people caring about them deeply and, you know, answering their queries and questions and helping sort of smooth that transition and de-risk it for people. So we love to get that message out, but we're, you know, we can get those products out and benefit everyone, the community as we contribute back.  According to analysts, the Office with Market Segment will grow at a yearly rate of 5% during the next five years to reach a global value of $30 billion, which, by the way, is the value that this market used to have around 2009, 2010. Where do you see LibreOffice in this scenario? Well, yeah, I mean, we need to continue to grow. It's good. I mean, Collabora is growing at a small multiple of 5%, so apparently we're gaining market share according to those projections, which is exciting. And, you know, we have a great product already. So I mean, I position LibreOffice really when I think about it in five years, not only as a PC product that you can use, but as a fantastic technology and a foundation for building productivity, document applications, anything, you know, generating PDFs, doing invoicing, calculations like there's so much richness in that technology underpinning. And I think that's why I'm most excited about seeing that grow and spread into even more places. Since the launch of the LibreOffice project, the end of desktop productivity has been predicted several times and actually even before, but the applications are still alive and it looks like growing again. Which is your opinion on the future of this market segment? Yeah, so I think that's the exciting thing about LibreOffice is really its maturity and its completeness.  And it is easy to write a word processor. It's just not easy to write one that can interoperate and handle that huge corpus of, you know, the whole industry's technical decisions. And it's quite interesting to see the ping-pong there, you know, one that implements a feature, another app then interoperates with it. And just this sort of ladder effect of growing functionality is then reflected in file formats and just the depth of complexity of coping with all of that. And I think LibreOffice as a technology base just has the most wonderful, mature and complete interoperability story there of any product, you know. So I think that's really, you know, that's really just a killer feature and it's never going to go away. And, you know, of course, obviously on top of that we built a collaborative line, you know, reclaim your digital sovereignty over documents and workloads and so on on your own hardware and network under your control. And, you know, we see lots of examples of that being really useful. So, you know, being able to control your documents, you know, whether it's the diagrams or the technical specs of a new car you can then show to your sales team. But without them being able to copy and send it on, you know, with their name carefully watermarked on the server overall of it. So we see that, you know, continuing to grow and reusing the technology there. But against that, I think, you know, users still will always want a PC desktop product. You know, I'm excited to see more PCs being sold, more laptops out there. Keyboards are awesome. You know, we should then use Fact Client's PC apps as well. During the last 18 months with the pandemic, Open Source Software and LibreOffice also have helped people working from home and this has increased the global number of users. Do you see this as a trend also for the future when the pandemic will be hopefully over or it was just a kind of specific? Yeah, who can say. So, I mean, I've worked at home exclusively for 25 years punctuated with conference travels to meet my friends like at the LibreOffice conference, which is exciting but around the world telling people about the goodness of LibreOffice and other software beforehand. So, you know, it's easy to work from home with a free software stack and do it from ground up. We've run an entirely distributed company, nearly entirely. There are a few locations in Cambridge and Montreal, but, you know, 150 people are working using Linux-based and of course, Collaborate, LibreOffice-based on top of that. I mean, that runs our whole business, you know, from finance, accounting, HR and all of the shared functions to the engineers are doing stuff. So, yeah, it all works. It works from home. And I think there's actually a real strength in not having clusters of people that communicate more at a higher frequency in the office. So, actually, one of the best ways to do this is to have everyone working from home. You know, it works well. And, yeah, but in that context, of course, there's also home PCs and, you know, people wanting an office suite. And so, you know, it's really thrilling to see LibreOffice being useful to people there and, you know, all of our hard work being used and appreciated by more people. So, I think that will continue. People will work from home. They love it. It saves a fortune in commuting fees and loads of time in hassle. And you can be more productive in many ways. So, I think everyone's going to work at home for some portion of their life, unless it's a very manual task. Which are the three best characteristics of LibreOffice that make the software stand out against the competition? Of course, if you have more than three, it's okay. But just to summarize something that can stay in the mind of users or prospective users. Yeah, sure. Sure. So, I mean, I think one of our huge strengths is our community. It's an open source project. It has multiple vendors and a huge diversity of contributors from all around the globe. And that means that when I look through my career, you know, I've been in many different companies. But my customers have had a continuity of support and services through some quite amazing industry upheavals, you know, through some micro systems arriving and disappearing, turning into Oracle. Now, IBM, you know, and Novell, they've had a great deal of attachment, micro-focus, you know, being spun out to Calabra. And those people still have the same excellent support and services that they've grown to expect and appreciate around the code base. So, if you made a bet on it, it's not there at the whim of any one company or what choice you make now. I think that makes it a very safe choice.  And the software just keeps improving as we see. So, I think the open source list is probably my top. One of the things with Calabra's mission is to drive open source. So, I had to try my second thing there in terms of the power to drive your own destiny around that. You can choose your upgrade cycle. You can choose your UX, you know, you can tweak it. I mean, we have customers who we've worked with closely. Quite difficult to deal with probably because they're extremely directive attention to detail. You know, we want your mobile phone version to have buttons that look exactly like this and do this and we're not happy with this particular interaction and we can fix all that. There's nothing that can't be fixed. I think that's really powerful and you can't get that elsewhere. Really, it's amazingly powerful. And then I think thirdly, probably just this technology base, the LibreOffice technology to build products on that. And the joy of being able to bundle and own this and put it into your own product is really incredibly powerful. So, lots of people need an office piece and you know, you can buy it in from somewhere else or you can use someone else's web service or you can try and reuse a Microsoft Office product in some strange way but you're going to end up with lots of strange deployment problems with API changes that you're not able to keep with and that are forced onto you by the exigencies of that and of course in terms of licensing it's complicated. So, I think having that base and collaboration online, we've got some like 50 plus million Docker image downloads everywhere, they're driving that collaborative document and getting it embedded everywhere from CERN, I guess to probe the mysteries of the universe to these business users sort of file sync and share needs, Moodle, it's there for people with their homework assignments for their students and you know, I think open source really is the payload control of your destiny and collaboration with others around your documents. I think LibreOffice delivers that and collaborators thrilled to be part of that story and to produce products based on the technology and to sponsor the conference. So, I encourage you to come and see what we're up to. Okay, thank you, Mike.


all right so what's up you guys Sedona um i want to talk to you guys today about just kind of like where i'm at and what i'm feeling so i had a video prepared and ready for you guys um it was already scheduled and stuff and then i woke up this morning and like i don't want to post that because that's not how i'm feeling today uh so if you're ready for a little bit of real um and i think a little bit of motivation as well uh this video is for you all right let's hope you guys sit down welcome back to my channel so i'm on my way to work um and you know honestly i woke up and i didn't necessarily want to go to work i i realized that um i i had like another sleepless night uh i haven't been sleeping well um my gi stem has been like all kinds of weird um and you know for those of you that don't follow me on instagram um i posted on instagram that about two weeks ago i lost my dog that you know has been with me for 14 years since she was a baby and it was unexpected it was pretty tough um because i had no idea that that was going to happen um especially when it did uh but you know that's either here or there like we we still had i still had to like go on with life so like i still had to like have my daughter's birthday party still celebrated my 14 years of marriage um all while you know in this process of mourning and then i still had to go to work and um take care of people that seemingly didn't want our help at the at the time just because they were enabriated or intoxicated um and take care of people that were in some of the happiest moments of their lives because they were having babies um and you you don't want to bring you like your feelings or your sadness or your frustrations to these moments because they just compound them or they they sour them um and so i wanted to talk about healthcare because i realized like you know what i'm not okay um i've been kind of going through the motions for the past two weeks but i'm i'm not okay and there are days when i am okay but today's one of those days when i'm not okay and i had to come to the realization that it's okay to be not okay um and to be able to acknowledge that and understand that where i'm at at that moment in time is actually pretty important so if you're a PA student a pre-PA or a PAC and you've realized there are moments when you are not okay or you're recognizing that there are moments or times or in a moment you're not okay that's okay it's okay to understand that um in my sleeplessness last night i listened to a message uh and it was talking about mindset uh and how mindset affects not only your mission but your ministry and you know my mission is i feel to be like the best PA in whatever specialty i'm in at that moment um and my ministry is to do that in a way that i'm able to show and exude christ uh without having to be blatant about it just being nice and being christlike and so for me um i think that the the moments when i'm not okay can affect that mission um where i may not necessarily want to be at work or do work or whatever the case may be in that ministry where i'm just down like i'm not being christlike and i allow frustrations to kind of just overtake me and so i wanted to talk to you guys about um your mission your ministry and your mindset you know and your moments of not being okay recognize it and and work at um talking to people or doing things that will allow you to to get into the right mindset because your mindset is very important in affecting not only your mission but your ministry okay so i just want to talk to you guys about that and let that out um you know pray for me if y'all are you know if you guys pray uh as i continue to like navigate this time that i'm dealing with and just you know continue on with life thank you guys so much for watching if you have any questions for me leave them in the comment section below don't forget to like and subscribe and follow me on instagram at it on the pa i will talk to you guys next time bye now listen bye


So, just to feed off the last few comments, there is no shortage of work in the world today. Indeed, there are more people working today than ever in human history. What many people, unfortunately, in the middle of this country want is to have work which requires them not to move and have no competition from people working very hard in China and other places. What they want is for the work to be provided to them on a silver platter at their convenience where they are. If you go to North-West Arkansas, there's plenty of work in North-West Arkansas. There's plenty of work for welders in California. There's plenty of work in lots of places, not in Southeast Ohio. So get in your car and drive to where there is work. They used to be what Americans did. But it is this claim that you should resent the rich and you deserve and you're entitled and your need justify that we provide you with stuff. It is the entitled mentality of much of Middle America, unfortunately, which is driving them to sit on their butts and to wait for the work to show up for them on their doorstep instead of doing what Americans always did, which is get up and create the work or go to where the work was. And this is, I think, what has happened in this country since the war on poverty and even really since before that. We have generated, because of the welfare state, because of massive distribution of wealth and because of the expectation that the government will meet every need and that it is morally obligated to meet every need of the people. We have created a mentality that is today driven more by envy of success than by ambition for success, more driven by resentment than by love of work and ambition. And as a consequence, we're getting the kind of politics that we're getting and we're getting the kind of mentality, I think, that is a real problematic, a static mentality that never existed before in America. Let me just, what concerns me at the end of the day is individual freedom, is individual liberty, is the ability of the individual to live his life as he sees fit, as he sees fit. Without a coercive government, a government that tells him what he can or cannot do, what he needs a license to practice to open a nail salon, they have to pay $20,000 to open a nail salon, or to get a license from the government to shampoo hair in California. You want to help the poor, you want to help ambitious people everywhere? The solution is to get the government out of our lives. And to really change our moral code, instead of waiting around for our needs to be fulfilled by others, instead of morally expecting, demanding that our needs be fulfilled by others, we need to return to that individualism that was, I think, made up kind of the American character which was to take care of ourselves, to have personal responsibility over one's own life, personal responsibility in the deepest sense, to make your own life your own moral responsibility, to live your life to flourish as an individual human being, no matter what your background is, through your own effort, your own energy, your own skill, your own ambition, to live as a human being, to live as an individual.