When you see the narcissist after the breakup, when you see the narcissist after you've left them or they left you, it might be a few months after the discard phase or even a few years. You're out somewhere and you see them. When this happens, there are a few ways that they might respond and this will depend on the type of narcissist you are dealing with and also, if you've managed to heal since you were with them. When you see the narcissist after the breakup and you're still going through it, it's only been a few weeks or few months. You still feel attached to them. They are going to take advantage of the situation. It is not going to affect them emotionally. It's not going to hurt them to see you again because narcissists don't experience emotional attachment. They are unable to experience feelings of closeness and affection and that is why they have difficulty sustaining meaningful relationships. That is why it was so easy for them to leave you as though you never meant anything to them because they were never attached to you. They are self-absorbed and lack empathy. They cannot share your experience. They cannot understand you. So it was only natural that you are going to grow apart, but they're not just going to let you move on gracefully. Many narcissists will stalk and harass you.  It regulates their emotions and boosts their self-esteem. It makes them feel as though they are worth something. In their minds, if you are interested in them, that means they must be something special.  They just act it in different to control you. And that is why they will desperately try to feed off any attention or admiration you give to them. Especially if they see you after the breakup. Many narcissists will dress up just for you. And do their hair just the way you like it. And then show up where they know you're going to be. It's all just to get your attention. They have this insatiable need for attention. But one person's attention is never going to be enough for them. When you see the narcissist after the breakup, they may show interest in you. They may act like they want to get back with you. But it's all an act. They cannot experience emotional attachment. And they do not value loyalty or commitment. Which is why they were able to leave you at the drop of a hat. They were able to leave you as though you never meant anything to them. Because they never had any interest in you as a person. They have no interest in anyone as a person. They don't even see people as people. We are all just objects to the narcissist. Things that they can use for their own benefit or gain. If they believe that you may have a useful purpose to them. They will take advantage of that. But if you're still in the same condition as when they left you. They're just going to string you along. They will try to make you believe that they're interested in you. But really it's just to feed off your attention. To make themselves feel like they're something desirable or attractive. Because people are just objects they use to elevate themselves. How the narcissist will react to you after the breakup. Depends on how the relationship ended. If there were other people involved. The narcissist is going to be less likely to contact you. Although the narcissist may value their sources of supply. Nothing is more important to them than their image and reputation. Everything they do is determined by how it will look to other people. In many situations towards the end of the relationship. The narcissist will have enforced flying monkeys. And started a smear campaign against you. So even if they wanted to come back to you. It would be very difficult for them to do that.  They've locked themselves into an undesirable situation. Because while you may have healed and put yourself in a better position. And they may see you as more desirable and attractive now. If they were to come back to you. It would look bad on them. Because while you were away. They were playing the victim. And telling everyone that you did all of these horrible things to them. They made you out to be a monster. So if they were to come back to you now. That would mean that everything they said was a lie.   The fact that you've moved on and done better without them. Already makes them look bad. But if they were to try to come back to you.  And they know this. So they'll stay ghost. You might see them out somewhere. But they'll avoid eye contact. They won't even talk to you. Because they have to trick themselves into believing their own lies. To defend their image of reputation. And deep down they know that you don't really want them. They know that no one would really want them. If they were to know everything they did with you. And that is why they hold on to their false sense of pride so strongly. That is why no matter what is going on in their lives. No matter where they're at.  They have to act like they're doing better without you. Because it's either that. Or they let you see the real broken mess that's inside of them. And that's just something that no narcissist is going to do. They cannot be vulnerable with anyone. They cannot experience an emotional attachment. And that is why it was so easy for them to leave you. They acted so cold towards you. Because in their minds that's a sign of strength. That means that they're strong. When really. They're only acting that way because they feel weak. They put up a wall to protect themselves. When it is that wall. When it is that wall. When it is that wall. That prevents them from discovering who they really are. It is that wall. That prevents them from ever maturing emotionally. From ever establishing a deep emotional connection. Love and intimacy. It is the reason. Why they will remain stuck in that condition. For the rest of their lives. When you see the narcissist after the breakup. They might string you along. And make you believe that they want to get back with you. But deep down they know that someone like them.   And that is why they always felt so uncomfortable around you. They targeted you because of your qualities and abilities. Because they believed you were something great. But it was those qualities and abilities. That later made them feel inferior to you. It made them reflect on their feelings of worthlessness. And insignificance. And in their minds. You were responsible for those feelings. You made them feel that way. Which is why they spend the rest of the relationship. Competing with you. They become very bitter and resentful towards you. They are very insecure. And feel uncomfortable around people. Or they perceive to be superior to them. They are more comfortable around people. Who are on their level. At least then there is no threat to their false sense of worth. But that is also what keeps them stuck in a position. Where there is no growth with development. In a position where there is no happiness or satisfaction. Because they end up trapping themselves around other people. Who share the same thoughts, feelings and ideas. Other people who are bitter and resentful. Which again is only holding them back in life. Everything these people attach to. Is only holding them back. While everything they walked away from. Everything they felt uncomfortable around. Was the only hopeful opportunity that they had. But due to their pride and arrogance. They throw away opportunities. They have an exaggerated sense of their own abilities and importance. Which is really just a coping and defence mechanism. But how they really feel about themselves. They live in a fantasy world where all they can do is pretend. But when they do find something that's real. They act like they're above it. Or they act like they don't see it. When secretly. That's all they've ever wanted. That's what they've been waiting for all along. The problem is. That realness belongs to you. And that is why they are so mad. That is why they become so bitter and resentful. So competitive. That is why. They will only ever feel comfortable. Around people who are just like them. Birds of a feather flock together. They know where they belong. And anything they extract from you. They will only take it back to where they belong. They're not going to try to build anything with you. Because they already know it's a hopeless situation. They know they're always going to feel uncomfortable around you. Because they're insecure. And they know that they're always going to be comparing themselves to you. To coexist with you. They would have to let go of their pride and arrogance. They would have to forget about what people might think about the situation. They would have to value connection over their image and reputation. They would have to choose you over their false self. And that's just something no narcissist is going to do. Thank you for watching. I hope this video resonates with you. Please like, comment, share and subscribe. Click the bell icon to receive notifications for my future videos. Check out the new website. www.narksviver.co.uk If you would like to donate. My PayPal link is in the video description. Coaching inquiries. You can email me at coaching at narksviver.co.uk Thank you for watching and I'll talk to you soon.


Live from San Juan, Puerto Rico, it's theCUBE. Covering Blockchain Unbound. Brought to you by Blockchain Industries. Hello everyone, welcome back to our exclusive coverage in Puerto Rico for Blockchain Unbound. This is the industry conference from people around the world, from Silicon Valley, New York, and around the globe. Coming to Puerto Rico, talk about Blockchain, decentralized internet, cryptocurrency, and really the future of society and global economic value creation. Of course, our continuing coverage is focusing on us here. If it's 2018, our next guest is Mark Jeffery, CEO and co-founder of a company called Guardian Circle. Welcome. Thank you, thanks for having me. So you guys are doing something really interesting. So we, first of all, we like to geek out as Fred say we're alpha geeks, but we love IoT, cloud computing. You're doing something really interesting right now with Blockchain and this new decentralized internet around something of a critical infrastructure nature. Take a minute to talk about Guardian Circle's products, the coin, token that you're doing, and what it all means. So, Guardian is the token. The company's called Guardian Circle. Together they comprise global, decentralized, emergency response. So, six billion people on earth have no 911. There's just, there's no magic number you can call, right? So hold that in your mind for a second. The other one billion of us, we do have 911, but it's not very good. It hasn't been really updated since the 60s. If you call 911, if you're lucky enough to not get a busy signal, they have no idea where you are. Your location information is not transmitted, which Uber can find you more easily than 911. Which is just insane, but that is the way it is. So never mind, so throw all that out. So 911 is broken. If you have it, it's broken and most people don't have it. So throw the whole thing out the window, let's start over, what would we build today? The way the world should work is whenever you're in trouble, no matter where you are on the globe, all you should have to do is press a button. That button sends an alert up to the cloud, the cloud looks down and sees what people and resources are already nearby. And then activates, coordinates, pushes all that, helps you as quickly as possible. So 10 people in three minutes. That's what we're, that's our- So there's a couple things going on. So to me, when you say, what should we start from scratch? But in my little operating system design, network solutions hat on, all kind of rolled into one is a stable, fault tolerant, resilient, robust, always on network. Yes. Database that is fully interoperable and updated in real time. Yes. Of every number, every location, every person's capability to understand the discovery and resolution of a number. Yeah. So that sounds like the internet. That's- That sounds like the internet. Well, that's a little bit probably further than we're going right now, but yes. But the idea would be- Ultimately, you're correct. That would be the ultimate. So no legacy baggage, 1960s telco. We're talking about in mobile, in Africa, for instance, there's more mobile penetration than anything else. That's what they got. So every country has their own sovereign kind of architecture. Yes. Are you guys looking at it from a global perspective or a regional? Global. So we think that, I mean, this is, this thing should be mobile native, location aware, and the alert should go out to multiple parties. And the phone number is your identifier in this system, but it's effectively an IP based system, really. So you're right. We have to balance that against privacy, so you get to decide who is on your alert grid, right? So you have to emphatically say, yes, my friends, family, neighbors, and these subscription services, and if available, these official services. So blockchain can solve the immutability privacy issue. Yes. The decentralized nature of network effect is a dynamic that people look for in good deals or good architecture. That's in place. People have a social graph, interest graphs, connections. So the analog world is going digital. I mean, the old days was, is there a doctor in the house, but you were limited by how far you can yell? Right. So here you're saying, literally, if you connect properly, the users in charge of their data, they can dictate what they want to connect to or does that kind of how it works? Is it peer to peer? Yeah, it's sort of peer to peer. I mean, a lot of people miss hearing me a little bit and think that when you press that button, the alert goes out to everybody that's nearby, right? So total strangers that may or may not be trustworthy are suddenly coming. That's not what I'm saying. That is not what we're doing because we don't want to accidentally summon Jack the Ripper. Like that's, you don't want to make a bad situation worse, right? Yeah. So you explicitly invite people into your protection grid. We call them guardians since Guardian Circle. That would be your Guardian Circle. And you can have an unlimited number of them. So six, 6,000, you know, however many friends you have. Then we will also feature paid subscription services where you'll be able to subscribe to like your local EMT collective or your local license and bonded armed security. Or if you're in a remote corner of the world, you could subscribe to the guy with the truck who can run you down the mountain, right? When you're having medical problems. So it's going to vary depending on where you are in the world, right? We're also working with the Women's Safety X Prize. We're a partner. We're the back end of that prize, which is an IoT device contest to make a panic button device, right? So when you push the panic button, what happens? Goes into Guardian Circle. So how does a token economics fit into this? So I'm getting why it's tokenizable. How does it work mechanically? Do I buy tokens for safety? Is it like, I mean, take us through some of the use cases. Yeah, sure. So there's five different ways in which we use a token. The first one is obviously to create the emergency, to buy emergency response subscriptions. Now we're going to allow you or provide a way for you to, as a consumer, just swipe your credit card in the app. And in the background, you'll be purchasing Guardian tokens, right? And it'll re-up every month if you don't have enough in. It'll be that sort of thing. So you might not even really be conscious of the fact that you're using cryptocurrency. If you are, there's a wallet that will allow you to just use the cryptocurrency manually the way you do any right now, right? And so there's that. Okay, so continue. Yep. The second thing we're going to do, we think that giving will be a big behavior in our universe. So you're going to be able to send Guardian directly to a beneficiary in the developing world. And what's cool about that is it doesn't go through a government's bank or an organization. So remember Red Cross and Haiti can't happen here. And we're going to go even further than that. Down the road, you're going to be able to track every dollar that you donated as easily as a FedEx package. So you are creating a direct relationship between people who might want to help people and then a direct access for resources for the user. Correct. And so that's the primary, kind of a two major flywheels going on. Just like people sponsor a child, safety is one of the biggest problems in the world. In fact, some people say, it's got to Greg Hahn who says it's the number one problem in the world that all other problems flow from the fact that people in the developing world aren't safe. Why don't they have water? Because they're not safe. Why don't they have education? Because they're not safe. Lawlessness has to be solved first. Trust is a huge part of this too. So how do I set this up? Where are you guys in the system? Is there a product up and running? How do people get involved with your project? Take a minute to share that. Sure. So we have apps released today and they're distributed worldwide on iOS, Android and Alexa. We also have an open API that lets anyone plug any alert device into our grid. Obviously we have to, we want to know who you are first. But basically everyone's welcome. And so, and then our token sales site is at guardium.co. G, guard, I, U, M. Guardium. Guardium. And then guardian circle. Correct. Guardium with the M at the end of the token. What's the plans? How much have you raised? What's the story? Yeah. So we're selling $10 million worth of tokens which represents 30% overall, 33% overall. And we have 100 million tokens in the, that's it, we'll ever be distributed. It's on the Neo blockchain. So we are, we are, we're sort of different from a lot of other folks. Yeah, yeah. We're one of the very first Western, we're not the first. Neo has a good reputation of high performance. Yes. Is that one of the considerations you had for them? Yeah, without a doubt. I mean, we deal on emergencies. So our, you know, our tolerance for things like crypto-kitties swapping the network is very low. So yeah, so we liked what Neo had to say in a lot of ways because of that. I interviewed the crypto-kitties at Polycon. Interesting story. Pokemon moment for the, for the, for the internet's there. Well, congratulations, Mark. What's next for you guys? We'll get through the sale. How's the team makeup look? What's going on with the company? Yeah, get through. I mean, definitely get through the sale is the biggest thing right now. We're a small team of like about five people plus some contractors. The next big thing that we have on our agenda is we're going out to India in four weeks to actually test the XPRIZE IoT panic button devices on the streets of Mumbai. So it's gonna be, you know, so Guardian Circle plus the vice. A lot of dense environment, a lot of people there. Yeah. So let's talk about you. What is your background and that got you here? Is there wasn't an issue with scratching? Why this time? Obviously the wave attracts a lot of alpha entrepreneurs. This is a disruptive time, but why Mark, Jeffries, why now? Why Guardian Circle? What's the passion behind it? So, well, I started life as an engineer and I won't worry with all my adventures up until this moment, but in 2013 I became very interested in Bitcoin. Wrote a book called Bitcoin Explained Simply.  You're an author, speaker, same thing. Distinguished influencer. So that's how that side began. In 2014, basically my girlfriend at the time had a stroke. She's fine, but at the time she was all alone and she was on the floor of her garage and I took her to hospital, brought her back and afterwards I realized she was alone for about half an hour. If this had been a real stroke, this could have been very serious. She could have died, she could have been paralyzed and she was drowning in health. There were about seven people who were either driving by or nearby while this was going on, within a thousand yards. And she had no way to get to them. Yeah, yeah. And, and I also- A personal example of what you're doing. And I also realized the other component was all the help, I didn't know six, you know, there's five of the other six people. I just, they're her friends, they're not mine. But during her emergency, all of us need to be sharing location and in communication with each other immediately. And the importance of that just cannot be overstated in emergencies. Seconds count. And so putting us in instant communication so that we can coordinate a response was the second half of the problem. I initially did not intend to build an app. I went looking for this app. And what I discovered was there were a ton of panic button apps, but all of them neglected solving the second half of the problem, which is organizing the response. And getting people on in the same- Mobilizing resources. Yeah, getting everyone into a war room without requiring them to know each other ahead of time. That was the big thing. No one had thought of that. So- It's like, it's like rolling up services when you need it. Instantly, it's like a- Yeah, it's hot-hoc services. Compile everything in time assembly. Real-time assembly. Yeah, it's exactly great. It's actually a really good way to put it. No, but this is also pretty important. So it's a great personal example. Thanks for sharing that personal story, but there was avalanches with your skier. It's people who go rock climbing. There's all kinds of use cases where a mountain biker is missing and all kinds of- Yeah, remote locations are really- I'm scuba diving. Where are people? Where are they? Where do they last? So a lot of these things are location-based, and no one knows what the situation is. So the alerting is only one step to the value chain. It is. But I think- Sorry, you have a question. No, no, I was going to ask you- It is. How do you go from there? Well, I think there are a lot of, I think safety check-ins. I think there's other things that we can do. But the one thing that, the one lesson that I've seen again and again and again and again is that the companies that fail invariably, oh, the companies that don't focus always fail. So you got to pick one thing and be the best in the world at that one thing. And the emergency situation is our one thing. And that's big enough. Well, I think you had a great opportunity and we'll squint through the, is the evolution of this market grows. It's kind of a moving train, but the value proposition is legit. I was talking to Fred Kruger, your friend and colleague in the business. It's a marketplace of these days. So it's money and marketplaces. In your case, it's safety marketplace. I could envision a day with your services where I publish and subscribe to services that in the catalog. Yes. Hey, I know my risks. Everyone knows what they do in vanity or risk factors, whether you're jumping out of an airplane or double black diamond skier. I would love to go to Lake Tahoe or a mountain or a place like this and saying, I'm going to take some chances. Here's what I'm going to subscribe to. Why? You're going to have to subscribe to some extra services while you're down. Yeah, I would use Guardium. Like it could be a market. I'm just bracing them, thinking out loud. But I mean, that's the kind of web services framework you could bring. It's exactly right. Is that the way you guys are thinking about it? I do, I do. I'm so focused on the sort of food and shelter stage of our life right now. Yeah, get an ICO done. Yeah, we've got tons of all those ideas written down, but we're not just, we're not quite there yet. But when we get there, great ideas. Absolutely. Well, the use cases are changing because people's expectations are changing and now technology can meet these cases. So, I'm seeing a lot of social entrepreneurship being done that are coming in through the funding vehicles that never would have got funded on venture capital funding. Ever. Totally correct. Whether it's battered women applications, human trafficking, safety apps, stuff that can make money. Not be like a zillion billion dollar business, but really change society and makeup. You've hit the nail on the head. There are a lot of blockchain companies or ICO companies. This stuff, the venture guys would never fund it because their model doesn't allow for it. All of these things have to be Facebook potentially or if they just have no tolerance for it. And the philanthropy world is not incentive on economics and also when the project loses its grant or funding, the stack just gets thrown away. So this allows for sustainability for mission based investing and developing. So I see societal entrepreneurship categorically going to boom from this wave across the board. The world will become a better place. We'll have better companies. Mark, Jeffrey, Guardian, Circle, co-founder and CEO. This is theCUBE's exclusive coverage here on the ground in Puerto Rico for Blockchain Unbound. A lot of great stuff here. A lot of great startups, investors. Of course theCUBE 2018 will be covering all the shows. I'm John Furrier, thanks for watching.


How to introduce RPA into your law firm? Law firms have bought into the idea, an RPA is presently being implemented to simplify work processes in the legal business. Any large law firm that requires extensive documentation, as well as data authentication, will find RPA useful. Law firms need to stay in the loop of corporate board meetings, company incorporation, secretarial issues, investors work and without a doubt, these processes require a lot of documentation. Here are six use cases of RPA in the legal industry. Assess financial risk of a law firm. According to McKinsey's Global Institute, it is possible to partially or fully automate 56% of financial planning and evaluation processes, as well as 20% of risk management workflow. Risk reconciliation performed by robots is a notable example. It requires no human intervention. RPA is flexible as it is capable of retrieving and sorting historical case cost details into the firm's database. Not only that, but it can also manage the credit ratings of clients sourced from the transaction database, as well as accounts payable from the accounting department of the same firm. Build clients risk profile. RPA can quickly generate the risk profile of the client. It works with existing e-discovery, document management, law practice management software, or imported public databases. For instance, it can fill the risk assessment forms. Recoverable information may incorporate the client's product and services, business operation location, and the length of time of the relationship between the two parties, the client and the firm. After collecting the data, RPA can establish a micro database having legally held all documents and collect all pieces of information in a single organized file. It then runs the reports, verifies for completeness, and subsequently sort it. The output is a cohesive client profile. Guarantee legal compliance. A verified client can go rogue. Updating compliance checklists before or after a case secures the law firm against fines and sanctions. RPA can carry out due diligence verifications at intervals and flag suspicious transactions with 100% accuracy and 24-7th vigilance. The software robots are capable of filling the suspicious activity report, then ping it after completion. By crawling the records of the law firm, RPA can input data needed in the transaction, as well as the subject details. Organize data between the client and the fee earner. Data management is a big challenge for large law firms. We are talking about terabytes of data here. Law professionals and practitioners need to go through these databases to build a strong case. You need to store, retrieve, and manipulate the information as efficiently as possible. RPA primarily organizes the client's requirement and business to guarantee consistency across diverse formats and frameworks. RPA is capable of moving the right documents from the document management database of a law firm to an extra net that can be accessed anytime by a client. Reduce fee overruns. Handling fee overrun is a big concern for law firms. It goes without saying that fee earners need to be extra careful while calculating their fees. The practitioner, or the firm, runs the risk of seriously hurting their image each time they take a new project. A fee earner rarely factors in the time required to finalize on a case or the resources utilized in the court. Fee overruns cause disagreement, and also open up length expense disputes and related matters. Track and audit running expenses accurately. It is important to note that a claimant would receive fewer funds and fixed recoverable costs. This is usually common in case a judge perceives that the claimed charges are disproportionate to the basis of the case. Complaints will start coming once the margin between the recoverable and the eventual charges of the client begins to widen. It is now the norm for corporate clients to employ task-based bill estimation having experienced frustration with the block billing. It is recommended that law firms buy into the ongoing technological revolution by leveraging RPA. Don't be discouraged by the input needed to implement, instead approach the implementation from an individual-centered perspective.


Okay well thank you everybody for joining us. This is an event on sustainable capacity development. It has a particular focus for the Sahil region and one of the things I wanted to talk about is the successes and the challenges that we have in sustainable development and capacity building in the Sahil region and my background is in isotope hydrology. I managed the water resources program at the International Atomic Energy Agency and what we do is we use isotopes to look at the movement of water through the hydrological system and we use these techniques to build a picture of the water resources in different areas and to understand how we can use water sustainably particularly as climate change starts to bite and we see movement and changes in the way in which water is distributed. When we use isotope hydrology one of the things that we want to do is we want to transfer this technology to different countries to allow them to adopt the technology and better manage their water resources but there are a number of challenges in doing this not just in the Sahil but in all areas around the world. Sometimes technology serves a good purpose and sometimes it can be difficult to adapt depending on what the circumstances are. What I'm joined here today by three people who also work in capacity development in sustainable water resource but in different areas in different aspects. So I have to my immediate right I have Stefan Julenbroek who is the director of hydrosphere water and cryosphere at the WMO. Next to him I have Christoph Henrich who is a project management officer in the technical cooperation department at the International Topic Energy Agency and next to Christoph is Elizabeth Karadine who is currently climate adviser to the UN office of the special envoy for the Horn of Africa where she works on peace and security management issues but she has previously been in the Sahil region and many of the issues between the Sahil and the Horn of Africa are very very similar. So what I'm going to do is I'm going to start with Henrich. The reason is that at the International Topic Energy Agency whilst we spend a lot of time developing scientific technology and innovations to help us manage water resources we also need to transfer these technologies to member states and that happens via the technical cooperation department at the agency and they they work with the department that I'm in the Department of Nuclear Sciences and Applications to help bring these skills to two countries that need to adopt them. So I'm going to hand over to Christoph to say a few words about how the technical operation program works and how this helps to build capacity development. Thank you thank you Jody very glad to be here so if it comes to capacity development in this area and actually overall when we look at how technical cooperation in the agency works I would say that one of the unique features of our assistance to member states is that we are really and that is not only looking into water areas but also in the other areas that we're really looking into building up the capacity in the country in order for them to sustainably operate these technologies going forward. So what we're usually avoiding to do is to outsource any expertise, expert assignments, publishing of reports but we rather really want to focus on building up these expertise in the country so that optimally the country once it has received the capacity picks up this knowledge and applies it sustainably in its own operations. Now of course this is a challenging work because all of us working in the development sector and looking into sustainability issues of development operations know that that this work is not easy in order to achieve actual sustainable impact and for example when we when we want to when we look into isotope hydrology and want to disseminate this knowledge into our member states we first have to actually work on improving the knowledge that these techniques exist because even among expertise among experts it is not often well known what these technologies and technologies can bring to the table in order to actually help countries to understand their resources better and once you have this knowledge that you can generate by using these technologies sustainably hopefully in your own operations it has to be ensured that this information is actually being picked up being picked up by the governments either in shaping the national policies or by other development actors that are designing water projects in that area. So events like this one here can of course help to sort of disseminate this knowledge among the international community but also governments have to increasingly be made aware of this this intersectional and interconnected work which is very challenging to do so and thirdly we we have to we have to further increase technology exchange and capacity building and utilizing their particular regional expertise so in regions that we work that share similar problems share similar capacity issues but also the same development issues or similar development issues to ensure that these countries start collaborating and actually maybe by the facilitation and support of international organizations but not or be laterally help each other and increase their capacity so that once this information that is being produced by these laboratories for example that also have to be able to ensure that they are actually working sustainable and actually that their work is being meaningful and being picked up so that they are actually being contracted later on. So I think one important statistics I wanted to say as well is that we had looked into this isotopodology linked to policymaking and in in other regions where for example significant low amount like not even 20 percent have stated of the counterparts that we have asked have stated that they are regularly using isotopodology for policymaking so there's lots of work to be done not only on the national but also on the regional and international level and I believe capacity building has to continue and continue and continue. Thank you very much Christoph. I think some of the points that you make about policy are really important that we can develop a lot of technology we can emphasize science and its importance but we need this taken up at policy level at government level and perhaps then I can hand it over to Elizabeth because Elizabeth you work with policy management peace and security aspects of water what would your be your perspectives on capacity development how it impacts the adoption of these types of innovative science and technologies at policy level. Thanks very much for inviting me to this discussion and as you said I'm working more on the political end but what we find in our work which is focusing on the horn at these days but have also applied in the Sahel is that when we're talking about very difficult issues of transboundary whether that's transboundary between states or between communities or other administrative boundaries or customary boundaries with communities or governments these are very contentious issues usually these are systems in the Sahel for example that are under various kinds of stress including climate change but also rapid social changes not to mention conflict and insecurity so what we find very much in our work in the more on the peace negotiation or side is that the technical entry points are the ones that are the most effective so when we want to to start having difficult conversations we find the technical counterparts whether they're in government or communities who can start to discuss what evidence do we have about the resources do we know you know anything about the extent of either groundwater or surface water resources do we know much about the use the answer is usually no but you usually do find very good technical people in all these contexts but with limited technological capacities like like you're saying when we start from that entry point you can build a lot of trust between the various parties at whichever level and start to really move towards the more difficult discussions of how you would allocate resources or manage across borders it's an approach we use in I can give two kinds of examples one from the Mocti region in Mali bordering Burkina Faso the example has been 29 different territorial entities trying to manage you know scarce water wetland resources their idea was to come together and create a plan sustainable development plan over 10 years so that they could have a starting point for investment and negotiation with each other what was decided quite early on was to also do a process of strategic environmental assessment alongside that to build the baseline gather all the evidence at a very in UN key of protocol standard was very important to them which at the same time as having that plan adopted by the state level then they also were able to have the SEA and the plan adopted at the same time which really gave them capacities and governance strengthened governance to implement and get additional assistance other examples that in less detail you know negotiations about transboundary water in the horn we really have to start them from a technical standpoint with technical members of those parties really as a trust building exercise and we always need more you know not necessarily human resources but data technological resources techniques that can help us to try to overcome assumptions you know clarify what the evidence is maybe bust some myths that can arise and move us towards progress on the thorny issues thank you very much Elizabeth you mentioned there at the end you mentioned data and this is something that we also deal with at the at the international Tommy energy agency the importance of data and data and information has been highlighted as one of the key accelerators for achieving SDG 6 which is well off target at the moment and capacity development will feed into trying to improve the achievement of that goal but Stefan if we might come to you the WMO of course has huge amounts of data it looks a lot at data and data management but also the WMO I guess is one of the UN organizations that really deals with transboundary issues because weather is not a national issue whether is regional continental scale so from WMO's perspective where do you see capacity development issues from from a weather perspective a climate perspective the actions that you can take thanks Jody a good question but I don't think the the main challenge is also different than what Christophe already said you know there's a huge tremendous need for more capacity building very much needs to capacity at the ground in the operational positions to really make a difference I think that is true for isotopes is true for hydrometrological data and and you name it yeah it's it's probably all the same at WMO what we are doing I would like to report one example which I think you want to have the main focus on this a hell today and what we do in the Volta Basin which is a basin it's a transboundary river basin which is shared between Ghana as most downstream country but then Mali Burkina Faso bit of Iori coast to go and be now a little bit I did I said he's here already but so it's going really a transboundary sharing of hydro of meteorological data is challenging but often possible but sharing of hydrological data is often very challenging and in many parts of the world however as you know the UN secretary general about half a year ago on world meteorological day he said well in five years from now we should have all people around the world covered by an early warning system to national hazards and then this is ambition in five years from now the wet wet 2027 we should all have all people of the world all covered now and then WMO was was tasked with the other secretary general of WMO of course and of course and here's some people helping that and then he was tasked with developing an action plan how do we how do we get that how do we get the half of the planet under early warning system because what our data shows that only half of the members of WMO have a kind of an early warning system that is up and running when it comes to floods and droughts and storms so very water related for this water based on one of our projects is it's I have to mention the adaptation fund who invests eight million for this project in this region is to cover this in a flood integrated flood and drought early warning system and we do not use isotope data yet so that's something I wanted to mention but overall it has been developed it's kind of an integrated model where you have static data and time series and you kind of combine that with the latest databases so to to issue warnings but it's not only the technological part it's also what do you do with the warning how do you inform local people how do you how do you alert them and kind of provide guidance for actions on the ground so it's really the end-to-end system from the observation down to the people who then have to act what we're doing this a lot of data is kind of integrated the data that is available but what is not done to the best of my knowledge in these type of systems is the use of isotopes data so I'm coming to your question was a long introduction is sorry for this but that's quite alright you can bring in isotopes anytime anytime and particularly I believe in the in the drought assessment drought forecasting understanding you know from which groundwater bodies that the low flows are fed getting understanding through the age of the system of kind of how vulnerable are the system how what is memory effect and what is the kind of the the amount of resources that is still there to make better assessments of future development of that droughts isotopes would be there but I do the best of my knowledge they're not used so so even if I I I applaud for that for all the capacity building you're doing but but it's still kind of a not not mainstream not operational in many many regions of the world including in the Sahel so that's a challenge that I'm fully prepared to take on we at the the IAEA are very committed to nuclear sciences as you know and I just think it's important to highlight how powerful nuclear sciences are in terms of water sustainability because nuclear science really we're just talking about sciences that occur at the atomic scale and in isotope hydrology what we are doing is that we're saying that instead of just having a water molecule we have an isotopic water molecule and you can have different types of water molecules depending on the isotope composition and we can interrogate the differences in those isotope compositions to understand the history of that water molecule how old is it where is it come from what was its source region and I think a lot of the issues that you're talking about in terms of early warning one of the things or one of the areas that we can contribute with isotope hydrology is understanding where the molecule has come from how long has it's been sitting in the system and what do we think is going to happen and be the response to these types of or to the types of events you're talking about floods and droughts what impact do they have on the hydrological cycle but one of the one of the pieces of information that we need for that is monitoring data right we need to understand how the system is changing what are the hydrological alterations so in the work that we do in collaboration with the technical cooperation department we for example in this region we we run a large project called the the regional Africa 7021 project and as part of that project we are supporting capacity development by investing in students we are funding MSc students we are funding PhD students to learn about isotope hydrology techniques to learn about the science how it helps with water management and hopefully those people who have that strong technical background will go on into the policy framework the government framework and help to bring the science into the mainstream but let me just ask you a question with a slightly different focus when we support those students they need to go out into the field and they need to collect a physical sample with the WMO as you're saying with with improving meteorological records a lot of the focus of the WMO has been on automation of information how do you think that intersects with capacity development because from our perspective say for example with isotope hydrology a lot of it is based on physically doing something with a student with them collecting a sample with them learning techniques and then building up this capacity and sending it through the system but what happens what are the consequences for example of automating these systems so there's no longer a person who physically takes a sample putting you on the spot there yeah no thank you very very good question i'm sure it depends on what type of isotope so are we talking about if it's a stabilizer tropes of the water molecule that that's easy like that taking a sample is relatively easy you need to protect the sample from evaporation as you're very well known but it's not it's it's not so difficult if we talk about gases that that's much more complicated if we talk about tritium it's also at least you need more volume and you need very advanced techniques to actually analyze it i the best of my knowledge only a year and a few a couple of others laboratories actually can do the analysis so it depends on the isotopes i the i have the feeling it's so rare the data and so the optimization of taking the sample is not is not the is not the obstacle i think it's the knowledge it's the last the lack of investment into these data sets anyhow if we get people interested in the data if we see if we if they understand the usability of it if they understand how much that can help them to to actually predict you know so much water is still somewhere in the catchment because we have um water of i don't know preseason or or or kind of paleo groundwater whatever so so really get getting a better understanding of the physical system and appreciating that then then we also find somebody to take the sample i believe so i don't think that the optimization of sampling is i would think it's not the biggest obstacle but it's more kind of mainstreaming that into our activity so it's kind of maybe i have a good ally i know the ia here is somebody who has some isotope hydrology background but also working at wmo maybe together we can make a difference and kind of get isotope data collection more into the into the operational monitoring systems yeah that's certainly one of our goals um with um it's interesting that the the different perspectives that we have on what are important what is doable what is not doable and this i i think comes into the issues of of capacity development what we can and we can't do and it's interesting to hear the different types of perspectives um if i come back to elizabeth now though um i know you have some background in water quality and this is this is a little bit um uh different to what we we've done previously we have increasingly um a strong focus on water quality because this is uh an issue that we see that has a very strong trans boundary focus in it because there are a lot of different um industries that are impacting on water quality as they come through how how is that tape being taken up at government level because i i think that water quality is increasingly becoming a very core issue uh for many governments worldwide and the the impact that deteriorating water quality has on the amount of water that is available to do specific types of tasks for example drinking water water for agriculture for growing crops for running industry what is there a perspective that you have on that yeah i think uh there's increasing focus on that at at the same time as you start to see in some of the countries of both the regions i've been discussing um more emphasis on environmental justice and climate justice and attention on you know environmental conditions um and where i have worked on it a bit more intensely is actually in the horn in the rift valley lakes around the development of agriculture and particularly flower farms um was my perspective working with the dutch government uh around the lakes of ethiopia and and kenya and to some extent uganda and i mean that's been a long term problem but we start to see a lot more um in interest of those three governments to address the problem uh and at the same time of the businesses involved uh whether you know private sector so to to join up on the planning around uh you know the use of the water but also addressing quality issues invasive species issues there's a whole whole range uh and voluntarily adopting some standards um you know as an industry uh to to try to invest uh private investment into addressing the problem of water quality knowing that the the business uh horizon is limited if if that's not addressed and i think the the pressure i mean you see new regulations and laws coming in around the the climate policies that these countries are adopting kenya and uganda for example have climate bills now where communities can you know hold their governments accountable um for issues that are affecting them related to climate change and sometimes environmental factors and you see those courses uh court cases being being uh you know brought forward and those are usually around forests or around water uh and you know the the whole sustainability issue often young people very passionate about this too so governments are responding uh to the change in emphasis thank you for that so let's just come back to young people kristoff um the agency has um a number of programs actions to support young people do you want to maybe elaborate on those i mean i know that i could as well but i think from that's one of the focuses of the technical cooperation is bringing that capacity and particularly on young people bringing them into the sciences how do can you maybe get provide some statements about how that works from technical corporations perspective so um as you have been uh already sort of pointing out before uh a lot of our capacity building activities and trainings that we offer are really hands on so we try to enable young scientists researchers to go into the field to go to a hosting organization and to actually stay with them for a couple of months even to really uptake uh and intensify the knowledge that can be transferred to them this is sort of what we call a fellowship and uh is our longest term individual training and we we also do of course national uh shorter term training courses where we provide the expertise from outside we do a lot of regional exchanges regional training courses that are maybe not as long but often also hands on and uh which enable the already mentioned by myself um um regional cooperation that is uh very very important as well um for example in uh the covid pandemic we were as i guess all development organizations that are doing capacity building um uh had a number of issues to actually implement our program um that as we were used to so back in those days we we we transferred a lot to virtual trainings and uh in in my in my projects actually we did a number of virtual training courses on isotopodology in this case for the europe and central asia region which haven't been existing before uh and because of that external factor covid uh we are continuing to do these courses as an introductory course uh to uh basically uh introduce the topic to some researchers it's not meant to be um replacing the actual hands on it's not even uh it's not meant to and it's not possible to replace the hands on long term uh in-person training but it can be a nice complementary feature uh um if you um uh to the existing capacity building suite or tools that we may have so as i'm listening to what we're talking about and i think it's just really coming out with what you're talking about now with the virtual training um one of the the the points of this particular session was to talk about capacity development in the Sahil and the particular challenges that it faces and we've talked about actually a wide range of issues um had a bit of one of those circular types of conversations that move from one topic to another but which of these issues are particular to the Sahil what is it every region has that's particular that specific issue those um those problems that are inherent to to that region what is it about the Sahil do you think that is particularly challenging and based on what you've just said now one of the things is obviously the ability to develop to deliver virtual training courses because there's a there's obviously that connectivity issue but what are the other issues that are perhaps particular to the Sahil that collectively i mean we have the IAEA we have the WMO we have UNEP represented here where are the areas that we maybe need to focus i don't it's Stefan whether you maybe want to comment on that with particular focus on isotope hydrology you mean or i think maybe yeah i think capacity in general yeah i i have to admit that i'm not the expert for that region but but i believe it's it's a combination of uh you know really least developed countries like Mali and Niger and some others and others are maybe a bit further on the development scale but also still lower middle income so i know what comes with it that in general there's always a huge lack of capacity building but also they even in my home country you know you have a lack of capacity when it comes to understanding the importance of isotope hydrology so if even some basic parameters for for closing a water balance are not already collected no then then more advanced nuclear techniques are of course and not the highest priority in many countries because people also underestimate and don't even know what you can learn with taking regular samples you know you to some extent you big word now cover up for for the lack of monitoring for the last decades you know because you you get a some insights about the passage you're not redeveloping time series but but you at least to get an understanding of systems behavior about interacting of for instance different depths or shallow versus deep of groundwater systems etc which is already very insightful for water management so so the the development scale is one of the things then is of course a language issue but that can be overcome i i believe with with the trainers that you can engage and and some some parts of there's a hell there's also just very serious security issues which um draw the attention to other things than than studying isotope hydrology because just you know human security is is already compromised in many parts as we know so that that does make effects of capacity building efforts even more more difficult i i mean i i think i completely agree with that and and water is um a main factor an important factor in regional security and this is what you really focus on right elizabeth um do you if we could maybe pass the microphone back to elizabeth i i how how can we better leverage technical developments technical capacity to help mitigate these types of issues well i mean like i was giving some examples at the beginning about how technical engagement and assistance can start a conversation towards something more tricky but i think in addition i really agree with what stefan's saying about the context of the sale is a low development base like it's important to remember i work in the horn now they're very much miles apart you know in terms of the the baseline of development having said and the insecurity and the political instability that comes with that which we see in the region is challenging because then your ability for technical expertise to to be you know incorporated into policy or for that policy to be implemented is quite limited under these transitional you know unstable situations we see in the sahel however i mean what's particular to the sahel you are asking is my experience is that there are excellent technical institutions and universities in bachina fasso in mali in senegal and you know depending how you in yuzhe that are ready to you know absorb such training and you know capacity development that you're talking about and i think there's there's many reasons in my work on peace and security where we look to the sahel because regional approaches are very they're very conducive for regional approaches to problems which other regions are not the context for cooperation you know is quite historical it's it's all countries want to work together as a regional block for the reasons we've just mentioned that that they're you know fighting for resources and attention a lot of the time very good at integrating across different sectors so you know what whether it's water management but also the question you were asking about how that intersects with industry and agriculture very you know there are mechanisms for doing that in that region and we look to it really it's for arranging the governance around those things we look to that region from the horn to see how we can model those kinds of governance or institutional solutions but you know there is a challenge i know from some of my former colleagues in the universities to now advance scientifically with the instability and insecurity that's really i mean you can't underestimate in the the five sahel countries is is really increasing so i'm not sure that there's a solution to that problem that's technological but i think the kind of cooperation at the UN or bilaterally you know partners could give can help very much with making progress under very difficult circumstances yeah i i see where you're coming from i think one of the one of the benefits or a non scientific benefits of the technical development that we do particularly with the regional projects and these new type of projects where we invest in memesy and phd students is building that sense of community amongst the people very early on early on as students getting them in contact with researchers students doing similar things in other countries and building a sense of community working towards common goals and hopefully the the expectation is that those people would flow through down into government and move up through government and maintain those links and that sense of community that's not just a local community but a regional community which i do think that you get within the sahel they have this sense of community within the sahel which i think is a very powerful a very strong component of their identity and and that's something that we can leverage to help build capacity there and one of the things i think we at the agency can do is in the context of a regional project we can look at investing in capacity in regional centers regional areas rather than in in each country and in areas like the sahel which as you are saying are very low development index we don't necessarily want to be working towards investment in every country we we need to be able to spread that capacity and i think that these types of projects that we we are currently working on where we bringing these people together in a regional context we are running regional training courses are really good at doing that stephen if i come back to you adaptation strategies adaptation in the sahel the horn of africa these are important um these are important developments that we need to do to need to bring to those areas in terms of a meteorological perspective or from the wmo's perspective do you have any insights into what are the core adaptation strategies that might be relevant for the sahel that something that we could look at from the ia ea's perspective about incorporating into the the the work that we do thank you am i allowed one more word on the the regional capacity building and i'm not forgetting the capacity excuse me the climate change adaptation question you know in one of my previous jobs we we also did regional training and bringing people together from from trans boundary river basins and yes you you discuss ideological modeling isotope techniques or whatever it sometimes it doesn't matter it matters in terms of what you want to achieve but but one thing which we shouldn't underestimate in these regional approaches and i fully subscribe to what you said is you you bring people together and it's not efficient to to have a little lap somewhere and then people go away they get promoted and then instruments are not maintained and and all these issues so to to concentrate this in regional hubs bring the people there together because they also build up these social bonds you know we we did this in mekong from in for instance with all the lower mekong countries as well as china and then after two years of a postdoctoral training on climate change adaptation it was so normal for them to from the chinese to write emails to the below mekong countries because they they collaborated with them they co-published papers with them and you know they really it's kind of these drinking beard conferences and all that which which really forms a bond and then makes it so much easier to to you know to do your best to exchange of course we have to perform in our in our policy settings but but still you break the eyes and therefore these regional approaches are are very useful in any environmental sciences when your climate change adaptation question when you think about water we are most of us like here as water experts today and if you look at climate change often there's a kind of a a bit of a bad story about water water floods and droughts they become more frequent and more severe and people drawn and infrastructures washed away and it's all kind of bad and it gets worse for climate change that is true but we have been saying that for how many years now and and were we able to really change the dialogue on that not not really huh so i wonder if if we kind of enter into that was a bit more positive spin from water is a problem of climate change and water is part of the solution to climate change and earlier this week the water and climate leaders which we a group that we facilitated at WMO and my group they published a statement for the COP and it's kind of really it's a and they call that a change of the narrative on water and climate change or an imperative on that and and it's really a much more positive side so if you invest in water it really can make a great interest a great impact on climate change adaptation you can avoid tremendous cost i spoke about earlier warning earlier today and the global what is it called the commission on global adaptation or what forgot the exact name that the guys in in order to them they made a ranking and and and kind of one of the best adaptation investments you can do is early warning systems they said so they they have these multipliers every dollar you put in pays back with the power with ten times so i'm not so sure about these multipliers of how true they are or sound but it definitely shows that investments in early warning can really pay back to society big scale or investments in water management to make society more resilient you know you create jobs through nature-based solutions you can help capturing carbon in the soil through certain water management with combined irrigation rainwater harvesting and whatever so there's a number of techniques which which are part of the solution so it's not only this um this picture climate change and water is a problem is the big problem it's it's kind of what are the water management techniques that we have available to be part of the solution and then in climate change adaptation it's basically water management it's to large extent water management yeah i i think i agree with that um i think there's a there's a lot of people who say that the climate crisis is really a water crisis having enough water of the right quality in the right area in order to do the things that we need to do to grow the crops we need to grow to have water to drink so so understanding how that system is changing and maybe as you say maybe we need to think about that in a more in a more positive sense rather than a negative sense um in the sahil i know the the conversation usually is around having too little water all the time and maybe i can bring up groundwater specifically because groundwater is in the sahil is something that we've been working a lot with looking specifically at where the groundwater is and what its age is to evaluate how sustainable the groundwater supplies are um and we um we the lot of the data that we generate there is very useful um to help people understand or different u n agencies understand where um where water is likely to be sustainable and can support development but also um in the sahil where we have moving people moving from one area to the other where we have unstable human settlements um what the impact on the groundwater supplies is going to be Stefan if i i just want to come back to you straight away because one of the adaptation strategies for climate is groundwater right people become more dependent on groundwater um and we use that to support diminishing surface water reserves do you think i know this isn't really a capacity development issue but it's a it's an issue that affects future um future um generations shall we say because do you think that groundwater is a sustainable adaptation strategy one of the things that we work with with isotope hydrology looking at groundwater is how long it's been there and a lot of groundwater systems that are being abstracted now are fossil groundwater systems and a lot of people are concerned about whether we if we are removing all of that groundwater and it doesn't get replaced how does that affect future generations and those future generations may be impacted in ways that are not anticipated right at the moment and that will perfect capacity development in future generations do you have a perspective on the push to use groundwater yeah no no thank you and groundwater but it's the year of groundwater 2022 huh by the way there is a you and water groundwater summit in early december exactly where jody and i i think we're in the same session but that's uh that that was my pr moment um it's certainly part of the solution and understanding the groundwater what type of groundwater is it how is it recharged how sustain what is the abstain no sustainable abstraction rate that we can do in a sustainable way um that is that is absolutely critical to understand so therefore in many regions groundwater can be part of the solution if you look at um previously i worked for a couple of years for imi international water management institute as director of water food and ecosystems and uh that we did some of these global analysis and uh for africa um it's around five percent that it varies regionally a bit what the irrigation source is groundwater it there might be some regions where it's more but overall average on africa it's largely surface water and in many parts of africa you do have groundwater resources that can be utilized if people know where it is um it could be uh just alluvial filling in in riparian rivers it could also be deeper groundwater and and it but really understanding the resource its characteristics it's what is the substantial abstraction rate could really help us and and really ensure um more food security in africa because i also as you said groundwater what do you do with it and and for and from a food security point of view it is irrigation is is a key thing that needs to be further developed as as a water as a climate adaptation technique so therefore groundwater is a huge role to play it's often underestimated yes often the groundwater is not exactly there where we need it and and um there's there's challenges but but it's not that use of surface water without challenges so so it's kind of a better understanding that these interactions between surface water and groundwater better understanding of the the resources and its characteristics and space and time help us to to more sustainable um use them but also groundwater is not the silver bullet as as you already implied in your question it it depends very much on local circumstances all right thank you for that stiffen i i think i'm gonna start to wrap it up now um if i if i take one thing away though and i think that made an impression on me is is the idea of the sense of community um capacity development is about building skills and we don't want those skills to be just in the moment skills we want those skills to last and bring about meaningful change and i think building those relationships and those connections that last a lifetime is a really important way of doing that and i think that science from my perspective and i fully acknowledge that i'm completely biased but i think science is a good way of doing that bringing that community sense of community together um i think that many of the issues that we've talked about now are not necessarily unique to the sahil um some of them are but some of them are general the issues to do with capacity development that we could focus on and i think if we talk about sense of community um also the greater working together and cooperation between our development agencies as well so if i might say Steph and the the work that you're doing we do definitely want to bring more isotope hydrology into it and work with you better on it Elizabeth would love to discuss more the the complexities to do with peace and security and the role that water plays on that and i know that christoph and i will have many discussions going into the future about how we can continue to build capacity development within the programs at the international atomic energy agency so i'd like to say thank you for the three of you for joining me and having this conversation which covered many different components of capacity development both here and now as well at the end they're looking towards the future and i look forward to working with you in the future on water projects thank you thank you thank you very much


Welcome to our video abstract presenting our Micropolitic Integrated Platform and Disposable Chips for Synthetic Biology. I would like to take this opportunity to introduce our work and show you the platform and how it can be used very practically. We've developed this Micropolitic Integrated Platform specifically for use in the field of Synthetic Biology. Synthetic Biology describes methods and technology to re-engineer biological units such as cells and microorganisms. To do so, bioengineers are using bio parts or biobricks which are encoding fragments of DNA and combining them together to re-engineer or reprogram cells or organisms. The design of new genetic systems involved the synthesis and assembly of genome segments. Biologists and bioengineers have devised a number of ways in which to assemble these large fragments of DNA. Ligation is one of them and in this article we have used the specific ligation protocol developed at GENCO Bioworks. Most ligation processes are using specific overhangs commonly known as sticky hangs and consisting of two to four nucleotides. Ligation allows one of the most efficient assembly methods which is pulling assembly. Compared to serial parallel or hierarchical assembly, pulling assembly has the advantage of joining multiple segments in one single reaction. Eventually the assembled DNA is meant to be transformed into competent cells and it is therefore crucial that the assembly is free of unwanted or unbound oligos to avoid losing efficiency and yield. We have chosen to focus our efforts on the automation of a double purification protocol. Current platforms used for the assembly or purification of genome fragments or liquid handling robots, they can be very expensive and high on maintenance. On the other hand, microplastic platforms offer advantages such as the mean miniaturization, high throughput and the possibility of integrating many actuators. Additionally, our system is very cost effective as it is based on a decoupling principle. The free layer is isolated in the disposable chip made of two elements, a block of PMMA which hosts the microplastic channels and valve and pump seats, and an elastomeric deformable layer which seals the channels and unloads the opening and closing of the valve via compressed air. The architecture of the chip consists of four independent channels, 250 microns in width and almost 4 cm in length. Each of these independent channels has inputs and output wells about 4 mm in diameter and a pitch of 4.5 mm much like a 96-well plate. Each channel has its own pump and reaction chamber and a back valve to isolate the reaction chamber. And now let's turn to the platform. The platform is made out of a PMMA block with threaded ports that accommodate connectors. The chip is fed of compressed air controlled by electro valves to close and shut the valves within the chip. We can add as many micro components as I want as long as they fit onto the platform without increasing the cost of the disposable chip. Here we have a pèretier and a robotic arm with a magnet. The chip can be placed onto the platform and clamped down. And silicone always ensures good connection between the chip and the platform. The chip can then be loaded using a pipette or robotic arm. We have performed the parallel purification of GFP, RFP and Plasmid by upon three assembled parts. Our biological validation study has shown that the relative percentage of cell number to exhibit the correct strain after purification on chip and assembly off chip is higher with the washing volume on chip. It has also shown that in on chip and off chip studies the percentage of relative cell number to exhibit the correct strain was comparable or up to 20% higher on chip in a negative control where the pre-assembled part was replaced with water. And the product of the purification was placed in a ligation with a Plasmid backbone. We failed to grow any cells or colonies at all. Thereby proving there is no cross contamination between the wells of the chips.