Good morning friends. Happy happy Friday. It's just about 6 30 and I just got to my WW workshop But I wanted to hop on really quick and let you guys know about my crazy week So as I've mentioned, there is a lot of changes happening in my life Last week was insane. Everything kind of hit me at once this week was much more relaxed because my best friend flew in Last Thursday and then left this last Tuesday.  We You guys there was so much exercise. So Friday we went and hiked Tubbs Hill, which is a About two mile hike in Coeur d'Alene, Idaho and it's it's a fairly easy hike There is some hills and things. It's a fairly long hike. It was gorgeous. It's beautiful there So we went ahead and we hiked there. I'm gonna see if I can't find some pictures And if I can I'll insert those here for you guys, but we hiked Coeur d'Alene Gorgeous had a wonderful time. We did pretty well on our eating on Friday Friday night, we had a little girls night barbecue to our house at my house And so that was really fun We kind of overindulged a little bit on Friday kind of called that the day that we're using our points day Saturday we got up and we went on a walk down By the river here where I live and it was supposed to be a two mile walk two mile hike It's easy. It was supposed to just basically be just a walk essentially so We go to do the walk and we turn apparently the wrong way and we ended up Taking over a four mile walk, which is just fine But it ended up being a lot more intense than what we had originally thought Not a big deal Sunday we just went on a walk around my house Sunday. We kind of took it easy We were a little sore and tired from friday and saturday and then monday We went to harris in Idaho and hiked a trail called mineral ridge and literally you guys it is all uphill You are climbing a mountain Legit seriously climbing a mountain. So we did that on monday It was rough my calves were really sore I actually had to stop and stretch them a couple times on the way up to the mount top of the mountain But once you got up there, it's absolutely gorgeous. So it was worth Every single solitary miserable minute getting up there But I and then and then tuesday we uh Took it easy. We didn't do any exercise other than just kind of like running around and around the house type of things But let me tell you guys It is friday and I am still sore my calves hurt. Oh I take that back tuesday. We went to the gym She drugged me to the gym on tuesday So I did a 30 minute circuit workout. So I was thinking wait a minute. I did work out on tuesday So my arms are sore from lifting weights. My legs are sore from the hike And then also doing the cardio at the gym. So It's friday, and I'm still sore. So wednesday and thursday. I didn't do any exercise. I was trying to Make my body a little less sore hoping to resolve a lot of the retention of water that I'm going to have in my muscles Before I weigh in today, but then it gets better on monday Shark week starts. So not only am I Crazy walking hiking exercising shark week starts. So I'm bloated. I have cramps TMI, but it's reality and this is real life guys So yeah, it was a week. All right. So as of today friday I'm still sore definitely not as sore as I was earlier in the week, which is a good thing And shark week is still here.  I don't know how it's going to go I feel like I worked my booty off and I should lose like 15 pounds on the scale But obviously that's not going to happen with shark week being here and the fact that I'm still a little bit sore From all the working out. So I don't know my eating was Pretty good this week. It definitely was not 100 I was extra hungry and I'm sure it's because I did so much extra activity that I really Like honestly didn't I I wasn't probably eating enough on my regular day-to-day eating So I just felt like I was a little hungry plus shark week We'll do that too. Yeah, but I ended up with over 300 fit points out of 50 this week So it was crazy. I earned myself a lot of fit points But let's see what the scale says with the water retention and being on shark week But let's see what the scale says. I'm really happy with my week. I feel good. I feel like my body is Toning up a little bit. It's actually starting to Shrink down even if I'm not seeing that on the scale from the exercise You know, I walk most of the days of the week as it is So I feel like my body is responding really well to the exercise But the scale isn't always responding so well to the exercise So we'll see what happens when I hop on so I'm going to run in go to my workshop Way in and I'll be back to share all of the results with you as well as this week's topic Hi guys, I'm out of my w w workshop And I am here to share with you the topic as well as my weigh in update. So first Let's talk about the topic now once again It is insane to me once again the topic is exactly What I needed the topic of this week was all about activity And as I mentioned before going into my workshop. I was the activity queen this last week so much activity I mean, this is the most that I've ever had on my journey so far I actually really liked it and I'm going to continue with the activity In fact, I'm thinking I may take a walk in the morning tomorrow Like I mentioned, I'm still a little bit sore But I kind of would like to go on a nice walk before I head to the grocery store tomorrow morning So loving the activity and I want to share with you some of w w's tips and tricks as far as Getting in some activity and maybe some new activity With activity what you're going to do for activity is just as important If not more important than how are you going to incorporate that activity into your life? We all lead extremely busy lives whether we work or stay at home moms We have you know some sort of schooling that we're going to we're all really busy So it's just about incorporating activity into our lives no matter how busy we are So w w's first suggestion is choose when you're going to do your activity Think about your day. What works best for you doing the activity first thing in the morning Doing the activity after work What works best for you to make sure that you're able to get the activity in as often and for the duration That you would like in the days that you want to do your activity Of course, it's recommended that you work out or have some sort of activity every day for 20 to 30 minutes But three times a week is definitely right on track of where you should be as far as activity So next ask yourself what gets in the way of you being active What is it in your day-to-day life on a consistent basis that you find yourself not doing activity? Is it that you don't have time? Is it that you're not getting up early enough? You're too tired when you get home from work or school And I would suggest that you take a time every day and you block it out Strictly for activity. So I walk every morning Front at 5 a.m. And I walk from 5 to 5 45. It is what I do my alarm is set for 5 a.m During the week I get up and I walk before I finish continuing it on with my day So I have a set schedule for walking and with being busy that is the only way that you're going to consistently get an activity That's why you find a lot of people have personal trainers Or they have classes because it is a set block of time that they are to be doing their activity So that they remain consistent in doing their activity by having those set blocks of time So pick a time that works best for you on a regular basis. Yes life's going to get in the way Things are going to happen where maybe that block of time doesn't work for one day or a series of days But it is best to set yourself up for success by just selecting a time During the day that's going to work best on a regular basis for you to do your activity Next up is take stock really think about What your time frame is for you and what's going to work for you during that time frame You really need to select when you're going to work out before you decide what you're going to do to work out So if you know for you the best time to work out for you is in the morning So say 6 to 7 a.m That is the time that you're taking stock in that's what you're committing to That's the time that you're going to work out Then make a note of that and plan on that so that you can continue to work out every day or the days that you've chosen At that block of time now we're going to move into what we want to do What activity is it that you want to do on a daily weekly Semi-weekly basis and lastly select an activity routine now that you've determined the time that works best for you Now it's to determine the what what are you going to do for your activity? So you are going to exercise four days a week from 6 to 7 a.m Monday through Thursday Those are your days that you've decided works best for your schedule that you can consistently do now What are you going to do? If you are someone that needs accountability You may want to join a gym and have a personal trainer that you meet with during that block of time Maybe you want to commit to going to a class For that block of time or maybe like me you just want to set your alarm So you get up and you go on a walk those four days a week from 6 to 7 a.m So do it for a couple of days evaluate it see if it works for you I think the most important thing to do with activity guys is make sure that it's something not only that you enjoy Because you're not going to stick with something that you don't like and also something that is going to work for your schedule So reevaluate it after a couple days make sure you're enjoying it And that it's you feel that it's going to continue to work on a regular basis for you for your schedule And there you have it you have a plan for some activity So definitely this week my challenge to you is set up some activity Maybe try something new or changing up your activity a little bit But really set yourself a schedule for your activity You'd be surprised by having that your mind automatically wants to get up get out and do that for me I'm just waking up by 5 a.m. Every day and I just roll out of bed and get dressed and walk That's just what I have to do. It's part of my routine. It's established into my routine now So it makes it easy. It's not even a question for me. It's just something that I automatically do So think about what activity you want to try this next week Set yourself up a plan of attack and I want to hear about it down in the comments Let me know what you think about maybe changing up your activity or setting yourself up for a schedule for activity So now let's get into this week's weigh in So as I mentioned, I have been an activity fool for the last week And I also mentioned that not only is it shark week, but I am sore my calves are incredibly sore My arms are sore. I'm sore But I had a good week. Now was my eating 100%? No I was extra hungry with all of the activity. But did I go crazy and overboard? Absolutely? Positively not. I definitely burned more calories than I consumed. I will tell you that for sure So when I stepped on the scale, I actually gained Point six and let me tell you this is the first time in my weight watcher journey That I've been legit disappointed in what I got on the scale. I definitely don't think I should have gained any weight I literally exercised so much that I don't even think it's humanly possible to have gained weight Even if I would have eaten terrible all week I still shouldn't have gained weight because I burned so many calories But because I didn't eat terrible. I burned all those calories exercising I was pretty disappointed when I stepped on the scale. I was expecting to see some sort of a loss but On the price your side on a side note I'm also having shark week and I'm also sore. So maybe that little bit of a gain I mean, it's about a half a pound. Maybe that is actually contributing That the soreness and the shark week is contributing to that because honestly, it's definitely not deserved this week. So I'm a little bit disappointed. I have to say But I'm also excited for this next week because I'm hoping that I will see the results that I was hoping for this week on the scale next week And to get myself kind of on a jump start considering I maintained last week And I gained this week to get myself kind of up starting and moving again I'm going to be doing the wendy plan again this next week. I had great success with it the last time I did it I'll go ahead and insert the vlog of the wendy plan right here for you guys But I lost 2.4 pounds the week that I did the wendy plan. I felt really in control I felt really good. I was tracking everything I didn't actually have a cheat day because I had to limit my points to remain In control and focused on the wendy plan. So I'm going to do that again This week we are going to dinner with some friends tonight So this is going to be my high point day on the wendy plan rather than tomorrow I've already tracked my dinner. I looked it up. I'm having two slices of pizza And today I can eat between 50 and 53 points So this is going to be my high day and I'm going to try the wendy plan again to maybe like jump start kick start The weight loss again, and I'm going to exercise I'm going to walk tomorrow saturday, and then I'm also going to walk monday tuesday wednesday and thursday So I'll get in an extra day Hopefully by then my muscles aren't as sore and I can just really boost the wendy plan with the activity So that's my plan if you're interested in the wendy plan I have an entire vlog that explains it and also there's a whole portion on my facebook group That talks about the wendy plan as well So i'm going to be putting my facebook group right here on the screen for you guys So if you're not part of it join my facebook group check out the wendy plan It's definitely used to break a plateau or to jump start your weight loss because essentially what it does is it shakes up your weekly Points in your daily points divides them out over the week So you're eating a different amount of points every day Some days are high points some days are low points, but it really just kind of shakes things up So i'm going to try that again this week. I'm going to see if it makes any difference Um in the scale next week and continue on with my activity per ww suggestion and again I'm really liking my activity. So I'm hoping that this helps with a loss on the scale next week. So Cross your fingers for me So thank you guys for watching. Thank you for all your support. Don't worry. I'm not discouraged I was just a little frustrated with the little bit of a gain on the scale, but I'm moving on. I'm actually over it I'm ready. I'm ready for a new week. So if you're new to my channel I would love it if you would take a minute and subscribe Hit the little bell so you're notified each and every time that I upload a new video Thumbs up this one comment down below I want to hear what's your plans for activity and I also want to hear how your week went Were you on track? Did you gain? Did you lose? Were you happy with what you saw on the scale? And was it deserved what you saw on the scale? Thank you guys again so much for watching Thank you for all of your support Let's kick this next week's butt and let's have a great loss on the scale. See you guys later. Bye


Thank you. I don't know. Is the mic working? All right. Cool. So, unfortunately, there's a problem with one video, but hey, that's life. We're just going to move on and get to it. So that shouldn't be the next slide. What's going on? Okay. That's a different slide, but fair enough. I'll go back to it in just a second. So first, I'm going to quickly say who I am. So my name's Midge. I'm a motion designer and 3D animator. I love education as well, so I do a lot of teaching, too. And I'm mostly interested in free and open-source software, IT stuff, pipeline stuff, a huge hardware nerd as well. I love to build everything myself. And I love watching movies and TV, so a lot of my inspiration comes from movies and TV generally, and got me into sort of being a motion designer. So what do I do? I make a bunch of weird art, basically. I love that motion design has become this thing where it's like, if it doesn't really fit in any category, you can just call yourself a motion designer, and it's like, looks weird, it looks abstract, it looks kind of techie, awesome. So yeah, I make all kinds of stuff. This is, I like to abuse all the tools in Blender as well. For example, this is all just one scene with different camera angles and a bit of lighting changed.  And it's one of the images I did when it got first sort of put into Blender. And my way of working is I really like sort of stream of consciousness working.  And a lot of that is where my work comes from. And this is a really good example. This is done with a hair system. It looks really big and huge, but really it's just a hair system, and you can abuse all these tools to make really interesting looking things. Fun fact, this was rendered before the hair patch, and I took a really, really long time, and then it got patched, and all of a sudden it rendered super fast. So, but yeah, let's see. Oh, gotta go back first. For some reason, the slides are in the wrong order and everything. I blame Apple. All right, so the thing I'm going to talk about today is days. Now, my birthday is about two weeks, and my brother's birthday is about a week before me, and about six months ago, we were talking to each other and we were like, hey, we've never really done a birthday party together. Wouldn't that be cool? And we were like, okay, we can definitely do something, and he does a lot of work in a local venue, and we managed to book the venue, and then we started thinking, well, what if we actually turn it into a party, and then, you know, we started thinking even more. I was like, well, you know, I'd love to, I've been moving more into the VJ scene and doing more, a lot of work with musicians, and like, maybe it's a good way to showcase some of my work. So, days turned into how I ended up talking to myself into creating 60 VJ loops. Yeah, because I started talking to people, and now we're going to have to go through all those slides again to get at the right one. Come on. You can do it. He really doesn't like it. All right, so I went from zero to 60 in four months, and I'm really glad there's going to be some render calculations and stuff in there as well, and I'm really glad I did all of this for the presentation after the work was done. Because if I would have seen a lot of these numbers, I would have gone, ooh, I'm not getting anywhere near that, because this is just like a giant pain in the butt, because it's all personal work. It's not paid, so I have to do other work in between as well to get it all done. And my reasoning here is like, I wanted 60, and I wanted them to be out 20 seconds each, so if you loop them three times, you get a minute. And that way, they're all at 2K resolution, so I can move around a little bit, and obviously I went for the cinematic 24 frames a second, mainly because I have to render less frames. And even if you calculate it out, it's 28,800 frames. And yeah, like I said, if I would have known this in advance, wouldn't have even started. But I'm really happy to announce that I've managed to finish it, not even before the party, but actually, like now, they're already done. So I'm going to give you a sneak preview of all of them. So that's the first video, I don't know. We need to do some juggling. And there's sound as well. No sound. No. Just pause it. This all worked at home on a Linux machine, all right? Look at all that professional gear failing. Am I the annoying guy with the videos? No sound. Oh, come on. I can try something. Ah, there we are.  Music was done by my brother, so I just wanted to give him a shout out as well. So, yeah. So how does a project like this get started, right?  And, well, you know, getting back to those 28,800 frames, you need to get it rendered. So I've got two workstations, Zeus and Rinsler. You can guess what my favorite movie is, most likely. And between them, I've got the power of five GPUs, and for some reason that doesn't feel like showing up, but there you go. So there's one workstation with three GPUs and one workstation with two GPUs, and everything, all those 28,000 frames, were all rendered by me on my machines over the last six months. And again, this is another one of those slides. It's really interesting when you do it afterwards because one loop is 480 frames. If I render them at one minute a frame, it takes about eight hours to render one loop. Now, the average was not one minute. I can tell you that right now. It was more like three, and I think towards the end it might have even started peaking towards four minutes. So the total render time, and this is just on one workstation, the main workstation of three GPUs, would be 60 full days of actual rendering, like 24-hour days. Now, I use the second machine as all a little bit, so it probably got cut down to about 45 to 50 days even. And I don't know how I managed to juggle this.  A lot of people might say, well, why didn't you use a render farm? Well, I tried, but the way I work, I don't know, there's something about it that render farms really don't like. And I tried render street one, for example, I was really excited about it, and then when you're used to rendering on a whole bunch of GPUs and then you have to fit your frame into half an hour on a CPU, it becomes near impossible. It's absolutely crazy. And even with the high power build of the last few months, it still came out cheaper to just render it myself. So it's been a very hot summer, not just outside, but it's been a lot hotter even in my office, so very interesting. But I'm really happy that I got it done. So how do you keep, how do you stay inspired through something like this? Because doing other stuff the whole time, I'm trying to push myself, trying to get better at it, and at the same time, not trying to get completely depressed and want to lock myself up and go completely nuts. So after I made a few of them, I started thinking about my methodology and I should really put some time into it to figure out a way to keep myself going. And the first thing I thought about was like, what's my target audience? Well, it's drunk people, right? It's going to be all projected behind the DJs and all over the place and I'm still figuring that out a little bit, but most people are going to be doing this and they might stare at it and other people are going to be doing this and staring at it. So it really releases you. It gives you some kind of freedom because you're like, as long as it's weird, people will probably be into it, which is really nice. And another thing that I found after just doing a few of them is I had to let my ideas go. I couldn't go in with a preconceived notion of what I wanted to make because I would just get disappointed if I didn't get there in time because you do feel the pressure. I can act really cool about it now, but I felt the pressure the last couple of months. So I found that letting my ideas just letting go of them and not trying to go in with, I'm going to make this or I'm going to make this was really, really important to me and really important just in general to stay motivated. And the way I started working around that is just by picking out tools in Blender that I hadn't used much and just going, all right, now I'm going to make a loop with this tool. I'm going to try and figure out how to loop it and just hope it works. And yeah, I did a lot of really fun stuff. So this is where the second video comes in. So this image is actually from a contest that was done recently on Remington Graphics. It's a YouTube channel. Grant Wilk there hosts it. He's a really cool guy. And the point of the challenge was to create something from a sphere without modeling or sculpting. When I read that, I was like, yes, I hate modeling and sculpting and not because it's not useful. It's super useful, but I've never been like a really good modeler. So modeling for me is too slow to get my idea out.  And it's just not me, I'm afraid. So I do everything through modifiers. So the contrarian that I am, I was like, all right, sphere challenge, I'm going to make a cube. That was like the first thing that came to me. And this just gives you an idea of the modifier stack that I use. And you see me sort of run through it. And this is my favorite way of working because I can generate ideas like that. I can change one parameter and all of a sudden I get a completely different result. You know, especially if you can combine it with stuff like particles and change seeds and start messing with things and really just iterate really, really quickly. And then you see the end result there. To get to that kind of workflow, I now know which modifiers are very prone to crashing and which ones aren't. So there's some of them that are really slow, some of them that are really fast. And it's been interesting to see some of the development happening lately on them. So what's the other part of that is you have to dare to fail. This is just a handful of stuff that didn't make it in the video and won't ever see the light of day because I didn't like them or they were rendering too slow or there was something about them that I just wasn't into. And this is just a very small selection. So I didn't create 60 loops. I probably created almost 120 at this point. But that comes back to what I was saying earlier about sort of stream of consciousness. I want to be able to work as quickly and as focused as possible. So if I'm working on something and I do this all the time, if I have something that's like it takes an hour maybe or a half an hour, I'm working in Blender. I'm not liking what I'm seeing. File, new, crash. Just throw it out, slash, remember and keep going, keep going. I don't have a very creative background. So mine is like creativity through brute force most of the time. And it does work. And some of them, maybe I'll revisit them at some point, but I think it's a really important part of being an artist. It's just daring to mess it up. There's nothing wrong with it. You don't have to show this to anybody. It's really important that you keep going for yourself and you become more critical of your own work. So there was going to be another interesting video. I don't know if it's this one. All right. So first, another thing that obviously pops up is you have to loop everything. And you have to get pretty creative at some point because after you've done use the same technique like a few times in a few loops. You want to start doing new stuff. You want to start looping new tools. And you have to really start thinking about how stuff works and how to loop stuff. And yeah, it's a kind of a brain melter sometimes, but some of the stuff that I've been able to loop is geometry. Very simple. It's a displaced plane and the way I approach that is I displace it. I then mirror it and then I can array it because it's got a perfect seamless shape, which is really nice. Another thing is a displacement modifier. If anyone here has ever seen my YouTube videos, I don't know what I would do without a displacement modifier. It's like the base of all my work. And you can set a modifier to use a custom object as a coordinate. So you just make a curve, have that follow the curve, and then when it loops around back to the beginning, you get perfect movement, which is really interesting. Next one is dynamic paint, which is fun too because it's like displaced with a little extra edge with some fade in it. You can do all kinds of things. So there's a plane under it that's being displaced and then it's being all pushed into a vertex group and then that vertex group is driving another displaced modifier. But it gives you really interesting looking organic feeling things and the trick to that is just having a lead in. So it's basically the same trick as the displaced, but you move your rendered portion over a little bit and have that lead in perfectly. And then I discovered this last one fairly late, but I have a feeling it's not going to load. They're not coming up, are they? So there's three more that unfortunately don't load. One of them is oceans. Ocean modifier is very loopable as well and the waves, which is a lot of fun. And then I've already forgotten what the middle one was. Wow. Oh yeah, cloth simulations. You can actually loop cloth simulations by exporting two separate point caches with a different seed and then mixing them together, which is really interesting. And the last one is particles. When I figure that one out, I was super happy. And basically it's a complete and utter hack. I'll use the particle instance modifier to get geometry and then I'll export that geometry as an alembic and then bring in the alembic twice and offset one to the other and have it sort of work together. If you're interested in this kind of stuff, I recently did a YouTube video on all these techniques so you can hear me babble about them for like 45 minutes or something. But I go through all of them and set them up from start to finish. And then there was going to be like a really interesting video where I actually had a time lapse of me making a loop. So just imagine me, like a blender screen and things going really fast. But basically what I was going to explain for this is that I never leave blender. I do everything from start to finish. So I start with all the modifiers and stuff and then I jump between shading, lighting, compositing and modeling because of those modifiers all the time and going back and forth, back and forth, back and forth. And because I start compositing really early, I keep my sort of end frame in mind as I'm doing stuff. And especially when I started using the Blender Cloud add-on to bring in HDRIs so I don't even have to leave blender. Yeah, it's awesome. I really love it. I don't have to leave blender anymore. So I've got this whole little toolbox that allows me to just create, create, create, create, create. And to me, that was like a really, really awesome thing.  Oh, there we go. Hopefully we'll illustrate this. And this is a very simple example, but being able to go back and forth, you can figure out where you're going to fix just in shading and rendering and what you can fix by doing compositing. So I just have a couple of compositing layers. They're not going to show, are they? Oh, there we go. So I abuse the mist pass all the time. Yeah, what is it? This one is sunbeams, I think. That should really be able to go inward. So any developers listening, please. There we go. More glow, more crazy stuff. And then I glitch it out and use the filter filter. I love the name of that one. You can really highlight your edges and do weird stuff with it. I know it's probably meant for other things, but I really don't care. Coming from a background of using a lot of proprietary software, it was really refreshing to see that Blender approaches the problem in a different way. It gives you all the tools, doesn't tell you what they do, which at the beginning is kind of weird, but something that the person that was speaking before me also said, not Eddie, but the other guy at Brent, I think it was his name, is I noticed when I'm teaching as well, students approach it very differently than like these streamlined tools that you're not supposed to use for anything else. So I really like that. And I just keep building on and then I put a scan line effect there. And the reason I'm able to do this is because I can just go back from forth to the pipeline without ever leaving it. And that to me is just absolutely crazy. I also come from kind of a VFX background, so I don't even render EXRs anymore. I just render my final composited frames to PNG sequences. Yeah, I know. But it works for me, so I don't mind.  So this is the final frame. So what I really wanted to say to everybody is just mess around and have fun. Like just break everything and then see what happens when you try to put it back together again. That's how a lot of my stuff kind of sort of ends up being. I let the tool guide me a little bit and then I take over when I feel like I actually have a concept that I can take out of it. Also, you know, use whatever works. I've almost completely converted to open source tools, which is really crazy. It's also very interesting when you talk to like an accountant and they're like, so you must have a lot of software you can like, you know, put in money-wise and then get money back from. I'm like, no, I actually don't have any software costs right now.  You're going to have to give everything to the tax man. I'm like, all right, that's what I get for going open source, I guess. So yeah, it's unfortunate we couldn't see the time lapse video, but yeah, I want to thank everybody. I've got a website. I've got Instagram, Twitter, all that stuff. There's a bunch of YouTube tutorials as well that I do. So if you're interested in this kind of work, give them a look or just say hi. It's all good. I've been trying to stream as well, which has been a very interesting experience. But yeah, that's it for me.


Dear students, in this module we are going to look at the side chain modeling as a very important step in the homology modeling pipeline. We have already looked at the template recognition and the alignment followed by the correction of the alignment and then backbone generation and loop modeling. So now you need to see the side chain modeling aspect of homology modeling. Remember we have already generated the backbone of the protein structure and placed those side chains that had the same amino acid for the target and the template. Plus for the gaps we have inserted the loops as and when PDB was guiding us. So now we are left with just one thing that is to place the side chains for those amino acids that are not the same between the target and the template. So everything else is done. So let's see how this can be performed. So as I just mentioned that the conserved residues that is the residues that work same between the template and the target have already been copied. So you now have a structure with the backbone with some side chains added while some side chains are missing. So the ones that are already added are because they had the same amino acid with the template. So this is your target structure at the moment. So now we are going to look at how we can insert these side chains. So the method is very simple. We insert these side chains by simply copying the torsion angles that is the alpha carbon and beta carbon angles to the target. So rotomers they tend to conserve these angles. So I will just show you an example how these rotomers conserve these angles in homologous proteins. So because of that there are certain preferences of amino acids and libraries have been built to show you which flanking residue that is the neighbor neighboring residue can be used to estimate the side chain position. So let's see. So here I have a backbone that is shown in blue. This is your backbone and this is one amino acid position. This is your alpha carbon and as you expect that at the alpha carbon position you have the R group attached or the side chain. So in this example you can see that this is tyrosine. So if you have tyrosine in the sequence then only two torsion angles are acceptable. So for tyrosine you only have two possibilities for the side chain. So the first one is in this conformation and the second one is in this conformation. So once again if you have tyrosine in the backbone of a protein structure that you are predicting and you want to attach the side chain then tyrosine only prefers two positions for the side chain. So this is the first one and this is the second one. So by looking at the preference of tyrosine you can set the torsion angle that is the angle between the backbone, carbon alphas and nitrogens. So these two angles are preferred and therefore you can attach the rotamer side chain like the preference. So now the next step is the optimization of this model. One would argue that we have properly constructed the backbone and then inserted the side chains as well as the loops and the turns followed by the insertion of the rotamers. So why do we need to optimize the model further? So the answer to that question is that once you insert the rotamers then the rotamers can create static hindrance or electrostatic interactions between them can cause the structure to become unstable. So therefore once you insert the rotamers and side chains you may want to evaluate your model for optimization. So as I just mentioned so the updated side chains can affect the backbone. So once the backbone gets affected for instance here if you have interaction between two rotamers then this needs to be optimized by shifting one rotamer to the other conformation and removing this one. So this is what is the optimization part. So you have to perform this entire process on the full backbone and that can only be done in a simulation where you consider the entire backbone along with all the rotamers and loops. The technology or the area that deals with such optimizations is called the molecular dynamics. So molecular dynamics or simply the MD simulations can help you to optimize the predicted protein structure. So in the MD simulations you place the protein in a force field that is you trap the protein in a region which is part of the simulation such that all the energy and the steric properties of the protein can be studied in isolation. So the model therefore is placed in a force field that I will just show you and all the molecules within the structure are followed in time to see how better they can suit to the predicted structure. So the large anomalies like bumps will be removed but sadly some errors will still exist. The golden principle in MD simulations is to minimize the overall energy of the protein. So if you minimize the energy of the protein it will mean that the rotamers have all settled into positions that are convenient and comfortable for them and therefore they are not creating instability to the protein structure which is a very good thing. Okay so here is your protein so this example is for Crambin so this is a cabbage protein and here in the box you can see we have trapped this protein which actually acts as an isolation chamber for this protein and we compute the possible conformations of the entire backbone as well as all the rotamers. So by doing that we come at a conformation of the protein that has the minimum energy. So the minimum energy protein is the protein that you will use as the result of your prediction paradigm. So in conclusion we have now optimized the protein structure as well and now this protein structure is your final prediction. So this will have some errors which will obviously be there because it is only a prediction but that you have taken care of the major sources of errors and minimize them. The next step is to validate this structure and that will conclude the homology modeling pipeline for you. Thank you. Thank you. Bye. Bye. Bye. Bye. Bye. Bye. Bye.


And in business news there's need for stakeholders to harness the value of facility management as it has potential to transform the nation's economy. This was the position of president of the International Facility Management Association IFMA, Shegun Adebayo, at the third addition of the advocacy they held in Lagos. Details in this report. Facility management is most often limited to the built industry but it goes further beyond that. It ensures functionality, safety and efficiency of the built environment by integrating people, place, process and technology. Like every other year the Institute of Facility Management Association IFMA put together this advocacy platform to engage in strategic conversations and discussions on matters of utmost importance to the collective interest of the profession. President of IFMA, Nigeria chapter Shegun Adebayo and his vice say the program aids a commitment to ensure conducive work environment and provide value through integration of people and technology in a place. Adebayo also spoke on the need to carry facility managers along when projects have been executed. And the advocacy of today is to create a platform for us to have conversations. Conversations especially around how we can actually ensure sustainability in built environment. So we talk about building problems. Of course, one of the key things we have been trying to also advocate is that whenever you are starting a project, be it building or what have you, because facility managers are more like professional undertakers. Carry along facility managers with you as your sustainability consultant. The solution that can come from IFMA on which we have been doing is the awareness and advocacy that we are doing and also to provide our advices to various government agencies and also to have a kind of a partnership with them in formulating their policies that can be of a great help to both the public and also the practitioners. The keynote speaker Fembe Akitunde who hinged his speech on the economy of facility management and the need to harness their value and impact in Nigeria says a good FM will result in better productivity and sustainable growth and development. FM has greater value in different ways. FM is a support business, is a support profession. What it does is that it starts by creating enabling environments for people to work, live and play. When you live in a place that is very conducive, safe and secured, your mind is at rest, you are more productive. Facility management follows you to the place where you work and it continues a good job by making where you work conducive for high productivity. Akitunde believes that when organizations are successful, it will create economic values explaining that facility management sits at the center of it all. Hello, hope you enjoyed the news. Please do subscribe to our YouTube channel and don't forget to hit the notification button so you get notified about fresh news updates.


Thank you my good friend dear friends all over the world and as we said we have a real full house today Mr. Geraldo Ruiz Esparza secretary of communications and transport of Mexico Mr. Jose Antonio Meade Curibrena minister of foreign affairs Mexico Mr. Gabriel Contreras Saldivar president federal institute of telecommunication of Mexico this is the newest member of our family I would like to give you a very warm welcome and thanks for the very strong collaboration with our team I would like to really especially mention that Dr. Geraldo Rojo Garza Alpare president national institute of statistics and geography Mexico my good friend Ignacio Peralta your excellency Makan Tanzania excellency professor Makan minister of telecommunication of Tanzania my good friend Dr. Sam Pitroda advisor to the prime minister of India Brahima Sanu director of BDT excellency's distinguished guests ladies and gentlemen it's again a real pleasure for me to be here in Mexico in this opening of the 11th world telecommunication and ICT indicator symposium I want to thank his excellency Geraldo Ruiz Esparza secretary of communication and transport Mexico for inviting us to this great country which is well known for his hospitality last year at the same time I was here for another symposium and we're another full house and of course we will remember our pretty potential conference in Guadalajara in 2010 which has re-elected me as secretary general I'm very grateful for that and my good friend Brahima Sanu was elected director of BDT and we very much appreciate the hospitality we think there is a inspiration there is a good luck that brings up that we can get here in Mexico and therefore it's always a pleasure to come back here excellencies as we meet today we should be celebrating the incredible progress that governments and industry have made over the past decade or so in 2003 when the WISIS process began where we were with just 1.4 billion mobile cellular subscriptions worldwide and there were only 780 million people using the internet today there are now almost as many cellular mobile subscription as there are people on the planet 6.8 people 6.8 billion people have mobile subscription and there are some 2.75 billion individuals using the internet through there are still this leaves us still with two-thirds of the world population still excluded from the extraordinary benefits brought by the online world but looking at the trends I'm personally confident that over the next decade or so we will see every household village school and hospital fully connected to the internet and that and the internet will be affordable this resonates with the targets that we have set in the broadband commission for digital development namely target one making broadband policy universal target two making broadband affordable target three connecting homes to broadband target for getting people online this is not just a pipe dream but a reality we need to achieve some of you might have already received and read the manifesto that the broadband commission released last week signed by 48 members of the commission last month I should say in September in New York or we are in December now just a month ago in 12 on 20 of September we released the manifesto and we presented this to UN Secretary-General Mr. Ban Ki-moon along before the president of the General Assembly in New York we are very pleased that some members of the broadband commission are here namely Dr. Sam Petroda who is one of our very prominent members of the commission and we are very proud of his participation here today at this conference some we you came a long way we all know the very strong contribution you're making in this industry and your presence here gives us a real real boost the manifesto explicitly states that broadband infrastructure applications and services have become critical to driving growth delivering social services improving environmental management and transforming people's lives the vital role of broadband needs to be acknowledged at the core of any of post 2015 sustainable development framework to ensure that all countries developed and developing a life are empowered to participate in the digital global digital economy I think all of us here gather in Mexico this week recognize the importance of measuring the information society before measuring we cannot we cannot track progress or identify gaps which requires more attention and we cannot identify new goals in underscoring the importance of the work and taken by the WTI s let me borrow the wise words of James Harrison James Harrington who said measurement is the first step that leads to control and eventually to improvement if you can't measure something you can't understand it if you can't understand it you can't control it if you can't control it you can't improve it I want to say that we can measure I cities we understand the technology and we can control it so we can improve it and that's why we're here today this thing is colleagues I'm gratified to see that this symposium has grown big and successful for the first time it has brought together ministers CEOs the director general of both telecommunications and national statistics entities and high-level regulatory authorities as well as high level United Nations officials this is a great milestone ICU relies on the cooperation of member states industry and experts in ICT measurement to ensure the production of comparable adequate and reliable ICT statistics our data I choose statistics for 200 economies and over 100 indicators comes together as a result of strong commitment from our member states administrations I say this guided by the lessons we have learned since 1889 when I choose started to collect and process telecommunication statistics I would like to congratulate Mr. Brian was unknown director of BDT our development sector and his team who have worked hard to transform what used to be a small technical meeting into such a great annual symposium I want to assure him that of my support as he continues to innovate for development as you may know he also recently launched three initiatives that have a far-reaching impact to the post 2015 sustainable development agenda and one of those is again chaired by some betrothed the M M help empowering initiative which comes at the right time for ITU doing just the right thing with our members and taking the leadership role and this is really have to be commanded excellency ladies and gentlemen this brings me to a very important issue the revolutionary potential for ICTs was not properly foreseen in the Millennium Development Goals as we must not and we must not let the opportunity slip away this time around as we are thinking of the post 2015 development agenda and sustainable development ICTs must be given much greater prominence in the UN's post 2015 development agenda as catalysts for broad social and economic development and that will means setting new goals and new targets and measuring these with new indicators I have personally been addressing this within the United Nation family just last September I was in New York in strongly advocating the need to use ICT as a sustainable development goal it's a tool for all the other sectors to move forward but he said it's an industry in itself that therefore he has a dual role that needs to be very much highlighted so let me ask this community to achieve to actively engage with this issue here in Mexico and to send a strong message to the world and to those who are directly involved in setting the post 2015 development agenda it is essential that everyone recognizes that ICTs will continue to play an even greater role in the post 2015 agenda then they have over the past 15 years and we need to ensure that they are specifically included referenced and measured as we move forward the WTIS this year is particularly important coming as it does as we are preparing for the final reviews of the MDGs the million and development goals and the world summit on information society which is plus 10 and as we look at the development process for post 2015 it's good for the developing countries in setting the targets for post 2015 million and development goals and it's good for the developed countries for setting the sustainable development it's good for all of us for the environmental sustainability we are here at the tipping point that is in gentlemen in our global society a tipping point where the pace of technological innovations has the potential to change the world for good and for the better our discussion here at the WTIS are an important part of that change and I'm very grateful to the governor of Mexico and the federal Institute of telecommunications for accepting to share the Mexican experience and they will tomorrow take us on a tour to showcase some of their successful ICT projects the Institute is newly formed and I personally followed the very thorough process that's the government grant went through in selecting the seven people top people in this in the very transparent process that has been set there and putting for the first time a real non-political known but national nationalistic with different backgrounds that are complementary as a commission and I can only see success coming in this and you can be assured of my full support and the full support of ITU in accompanying you not assisting we're not assisting anyone we are accompanying you in what you want to do best we're bringing the additional flavor of international experience of the good and bad experiences that are happening everywhere we are after all in the information society no one should make a mistake that was made elsewhere for lack of information in this information society and no one should reinvent something that was invented already for lack of information we are here to cross-factor lies to exchange best practices lessons learned good and bad so that we move fast so we all win that's the beauty of our industry we're all winners in it ladies gentlemen let me again for a express my gratitude to the government of Mexico for inviting us here I look forward to interacting exchanging views and debating with many experts in the room for the next two days I thank you very much