I mean, all of patents and all of that stuff, but it has to do with my experiences over the last six years, where we've submitted about 75 ideas into three companies. I have seen about 20 people submit IP. They're all agile teams varying from scrum sprint sizes of one week to four weeks. The people with three years experience to 15. I hold about two granted patents. I have two patents pending. I have seven defenses. So we're talking about a lot of it, but this is not about all of that. The philosophy is how many of you would want to have some sort of IP in your name, in your lifetime, about 70% raise their hands and 30% don't believe they will have one, but they can. So that smile say that because that is exactly the first reaction which I get. But that's the reason I put up these pictures. The reason being that most of us tend to think that IP is about software code and some magic moment where you will invent a light bulb. These are actual patents. I hold the one in the middle, the one there, and this is patent pending. And this one probably saved our jobs for at least 10 or 15 people for three years. So really what I'm trying to tell you is that this saved jobs and lives because somebody came up with an idea. When a VP put his leg up on a table and he said in six months he would shut us down if we didn't change the way we did things. The next one was when I was testing the product as part of one of the testing user stories which we were doing. That is actually the Sony world signal in Kormangala. If you've ever gone to Kormangala, it's that really crowded signal where you find it very difficult. It stops a lot and then there's that period between it. I used to work at Yahoo at that time. So you have the period from the Sony world signal to that army base where there's very, the phone signal keeps dropping. And as I used to keep testing it on my way to office, that is when I came up with the idea. And that's actually a granted patent on 31st December of this year. The next one is called serendipity because have you traveled to US? Several of you, right? When you get into those hotels, we see those sheeps of newspapers which are kept on the entry, does it get fatter or thinner on Sundays?  In fact, 40, 50 papers. So yeah, this one we filed about 18 months back when my team.


I'm JJ Zachary-Sin and this is the Fandal Hurry Up week three waiver wire. Let's get to it. I like Rondell Moore He's available in 65% of Yahoo leagues right now He's coming off a really big game in week two and so far throughout this entire season all two games of it Rondell Moore is leading the Arizona Cardinals and targets despite playing behind Deandre Hopkins AJ Green and Christian Kirk you got a love that Arizona is using Rondell Moore similar to how he was used in college at Purdue They're giving him those low average depth of target targets And he's using his athleticism to get that yards after the catch for that offense So I love Rondell Moore. He should definitely be added off the waiver wire this week I also like Emmanuel Sanders Sanders right now has an 18% target share in this Buffalo Bills offense Josh Allen has not been great at throwing the deep ball so far this year on throws 15 plus air yards Allen's completion percentage is 23 and a half percent Whereas last season it was 48 and a half percent Emmanuel Sanders. He has a really high average at the target He's the one who's stretching the field for this Buffalo offense But it just hasn't been there yet with that connection between him and Josh Allen once it's there Emmanuel Sanders could really blow up for fantasy purposes. So I really really like him off the waiver wire He's available in a ton of Yahoo leagues Finally, there's James White. Matt Jones is playing it a little bit conservative to start the season He's on the lower end when it comes to 15 plus air yard rate as a result He's dumping the ball off a lot to his running backs New England once again top five in the league and target share to that position James White right now. There's only two running backs with a higher target share so far this season Christian McCaffrey, Alvin Camara. James White, especially in PPR our format should be rostered. So go ahead and get it


uting for a second what you're saying but the other side of the story is what he's basically saying i think this whole open till 2016 greg there was very very little mention of irish unity it was not a hot button topic and all the rest but since brexit has become an everyday conversation the demographics in the north have changed big times the catholics or the naslas community are now in the majority shin fiends the biggest party shin and michel o'neill as a force minister uh but uh unionists are now in disarray and and there is talk that we'll have a poll for irish unity by 2030 and i think what harris is sort of saying to all those young people who think that uh rom and london and paris are closer than belfast and dais look we need to get engaged in a conversation about how we bring about unity and personal relationships are need to be embedded in a way so we all understand each other i think really what this talks to and it's a shame it's kind of attracted from what is actually quite a useful conversation that he's trying to spark here is that and and the bbc saw this a number of years ago in that they knew they were becoming too london centric uh and they they started farming out some of their news teams and uh uh production commissioning or program commissioning and production to manchester and other parts of of of britain we have a problem here it's there is a double and bubble and unfortunately it seems even politicians a lot of politicians from the west and more rural parts of the country when they go into doubling they actually they actually come under this bubble that you know the journalists and the politicians all talk to each other slightly hived mind unfortunately they really if they don't talk about decentralization but it would really help i think if if they moved some of the some of what they do in dublin beat journalism or politics out of dublin to try and get a better understanding that you don't put your foot in it like they did with that type of language for us hope here in the northwest convoluted as begara but i hope it made some sense yeah now greg i agree with you toby greg one third of the population of irland loves around that greater dublin area and it's our whole politics our whole journalism our everything that you can think of has become dublin centric if you went to our tea in the night you won't wonder how you do it took off like a bullet uh all those years ago because simply we had been ignored for so many years if they're doing a program about school meals it'll be a school somewhere near looking as they're doing a program about uh in our city you know poverty it'll be somewhere out of batalla or something they would never think of coming to donningall and talking about rural poverty or whatever you know i think most of the things this last while dublin has become so it's a wonder by the way this place doesn't go like a seesaw because dublin there's so much dublin's so so heavy these days and that other place is so light when i worked at it's when i worked at northwest radio the uh i said this is not a bad thing to say but i i'm not going to sort of not give an example i have one when i worked in northwest radio it was based in sligo and there was a satellite station in donningall uh and there was quite a good bit of program as well but you'd try and pitch stories for inclusion on the news you know what i mean and and and you you would notice that sort of the stories would be further down the bulletin than unless a story about sligo which would be further up but it would be quite frustrating i would say there probably is quite a lot of frustration amongst correspondents that feed into the national stations in actually pitching stories and getting them on the programming because there are brilliant correspondence right across the country but ultimately the editorial decision is still made in dublin and i think until such time as that changed we're going to continue to see the path of least resistance the handy stories the dublin centric stuff and greek i was a sub editor in our team many many years ago and i was actually on the desk one morning and there had been a shooting in the north somebody had been shot dead and there was a bus strike in dublin and i was the only sub editor on the desk that morning and i wrote the headlines for i think the half six news and the guy y'all jump flanning it was a lovely man by the way came in and changed it he said he put the dublin bus strike ahead of the shooting dead of somebody on earth and i said no i'm not arguing you're the boss but why did you switch it around he said pat a third of the people on irdan live in dublin the bus strike will affect them more than the shooting in belfast or yeah you can't remember where it was in fairness you know and that that was the reality and that that was that that was the the thinking behind switching my the headline i thought the shooting dead of a human being would be far more important on the bus strike but i was wrong apparently well what a way to leave it okay pat and yet was still here to this day and as i say and i think you perhaps a little more than i both accept that what the t-shirt was trying to say is important it certainly resonates with certain people of his generation to some extent is right and it's a good conversation to have unfortunately slightly overshadowed by this non-inclusive language that just makes a big yeah absolutely it could have told these words better that's for sure pat it is always a pleasure thank you so very much and you appreciate it take care of yourself pat mccart there author columnist podcaster former editor of the dairy journal the county's number one talk show the nine till noon show on highland radio the nine till noon show is brought to you by letter kenny credit union with monster loans available up to 60 000 euro for all occasions visit letter kenny cu.ie paco letter kenny are celebrating five years open this 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evenings or on the weekends let tfi local link take you there visit transport for arland.ie irish national opera presents a new production of low limpia day by the valdi a fiery opera about tangled relationships and forbidden love played out with the backdrop of the olympic games olivia award-winning conductor peter wheeling conducts the irish baroque orchestra one night only in ungreening theater letter kenny tuesday the 30th of april tickets from 29 euro see ungreening dot com funded by the arts council ryan adams is back on tour in 2024 join highland radio one our trip to dublin to see the man himself at the three arena on tuesday the 21st of may 2024 your trip includes luxury transfers bed and breakfast at the four star carton hotel blanche star your standing ticket to the show and a shopping trip to dublin city centre the following day find out more on the outlet at highland radio dot com or call us on 07 4 9 1 25 000 a lot to squeeze in the next 40 minutes including uh daniel o donnell he'll be joining us talking about an upcoming concert but first i want to say hello to an and an is going through unfortunately what an awful lot of people are uh going through across this region and i think particularly in certain areas of uh donnie gall and good morning to you thanks for your time thank you very much great how are you i'm doing okay an um unfortunately though you had to seek out an appointment with your gp uh this morning in the inner show and area what was your experience well i actually uh this was the second morning i rang i rang on friday morning um i feel like i could get an appointment to be told that their books are closed until after the seventh of may now i'm gonna have to hold your second i want to hold you and because just in case people don't get the date i want to remind people it's now the 16th of april an phoned the gp for the second time and was told that the appointment's book i.e an in-person meeting with the gp is closed until the 7th of may next month yes what else were you told um so uh unfortunate for me um i i have to see the specific gp because it's um this person that i have been seeing for nearly the last year uh and i am under a consultant in dublin rang the consultant in dublin this morning to be told that i can't be seen any sooner until the gp sends a letter with updated symptoms i have severe nerve compression in my neck and the symptoms are progressing and so um try to ring on friday and no uh this couldn't send me so i thought right you know i'll try and fold and hold out but unfortunately that's not going to work so i rang this morning uh between nine and ten its emergency appointments couldn't get through even though technically it's not an emergency uh so i got through after ten and to be told that the books are still closed till after the seventh of may and um this is right well i need to speak to somebody i need i need to be seen because i can't i'm stuck between the rock and a hard place and basically was kind of bob dawson the attitude to me was tough so i well if i rang tomorrow between nine and ten um will i be able to request my gp no you'll just get whatever doctor you get and i suppose that's not good enough so unfortunately for me i can't see my gp till after the seventh of may and even then there's no guarantee that i won't be waiting another month so everybody's going to be ringing at the same time looking for an appointment so you didn't actually so so you didn't actually get an appointment for the eighth of may oh no no no we have to run back after the seventh of may and i mean i suppose to let's talk let's talk about one on an emergency is uh your bulging disc causing the nerve pain this condition is getting worse you haven't had a day free of pain for over a year and also the lack of access yeah the lack of access to a gp is actually also delaying your engagement with your consultant so i mean if i was at an emergency to go you'd go to the ed right so what's class as a gp emergency i'm sorry i'm not a doctor but this sounds like a personal emergency to you an you need this you need to be seen yes i do and but that's that's where i'm caught you know and i can present an accident emergency letter again as i've done numerous times in the last year but unfortunately there's nothing they can do for me so i end up having to go back to my gp who has prescribed different medications x y and z and i was told i was going to be in dublin in january that i was going to get a nerve the cp guided nerve block in six to eight weeks i rang on friday to find out that that is not even possible the cp room in the hospital in dublin is closed since october and they have a backlog of almost a year so so i can't get the operation i get the injection okay so just to be absolutely clear the nerve block injection you can't get that till you you see your gp you can't get the surgery uh to until you get the nerve block and in the meantime your symptoms continue to worsen uh it sounds to me like an terrible ordeal to be living in constant pain for almost a year i mean what the problem is is what can you do this is also too by the way where this has an impact beyond the actual condition someone has because this is going to get you it's going to wear you down and no one is strong enough that they could put up with this it's going to wear you down and affect though yourself and those around you i'm sure yes as much as we're trying not to at this stage i'm sure everybody around me is sick of listening to me and not only that i will ask him i was in casualty i actually turn around and i told the doctor you know what i'm about ready to jump off a bridge at this stage and he kind of looked at me and i said i'm not actually going to jump off a bridge but i says i can honestly see how some people over medicate and accidentally overdose or how some people cannot take the pain anymore and unfortunately we wonder why suicide rates are going up that's another reason because people are in pain and they're not been helped not been seen and there's only so much you can take before you can take no more also to i mean obviously you know what we're just talking to you using your first name i think you've also spoke on on social media this is a gamble too because you would hope it doesn't but could this make it more difficult for you to get an appointment after the 7th of May could i haven't mentioned any names and anybody that knows me will have an idea who i who i attend but i'm it's not just my tp it's gps and then i shown and i know that's what i'm having talked to different people that go to different practices so it seems to be right but i just i don't understand it's the first time in my life where i've ever heard of a doctor having books and being closed when has this become a thing that they have books that they close and then there's no appointments until they open their books again when did this become a thing yeah a doctor somebody that you should be able to access as you need i know there are people that run and maybe don't need to be seen as often as they are and i know there's people like me that need to be seen and can't be seen and it's just so frustrating because um you ring looking for appointments and you met with people that maybe don't understand how much pain you're in and then uh can't see anybody and you know what do you what do i do do i take extra medication do i hope that my gp won't tell me right you know you shouldn't have spoke out so bye bye you know is there any cross border any cross border options and given your location um i'm not a hundred percent sure yeah you have dream with specific yes yeah yeah and i i'm i'm wonder um like i have been seen by um i come on a lot the actual consultant but has registered doctors and doubling twice but they it's almost like gaslighting because they're telling you just keep taking the medication and see how you feel or we can't do the operation till we see how the nerve block works but then there's no sign of a nerve block happening um i i ask them can they not just do the operation and be done with it because is it just going to work or it's not and if it doesn't at least i know this is something i'm going to have to cope with for the rest of my life you're just not going to unfortunately then i'm not going to bend those rules in that regard and and listen i mean obviously our gp practices in certain areas are dealing with a massive increase in population and an obligation also too to take on extra numbers in some cases as well yeah there was talk of this big fund to pump into certain areas as it turns out it's never going to transpire to sort of maybe increase you know gps nurses in certain areas where population has increased dramatically but that's not going to happen anytime soon if at all it's certainly not going to happen for you but you asked a really good question when did this become a situation this is like you know uh when did it become a situation where appointment box close at your local gp for the best part of three weeks when did that become a normal situation um and also a caller says here by the way i was in the same position recently and was told to go to the gp door in the morning the next morning and i already died when i saw the q doing the same thing when the doors opened it was like a sale everyone rushed for the reception desk it's no wonder there's no appointments when people call um yeah but that's another cohort of people who can't get an appointment so they're turning up as like it's a fire sale for health um i don't know what to say to you an um we you know that we get a lot of calls and i think i can i sort of identify which area is and a lot of them are similar unfortunately an but i do also recognize there's an awful lot of pressure on gps but you need this i understand that i do but also you're being disadvantaged so you wonder why like i mean this you are entitled to see your gp you're entitled to get a letter you're entitled to get your nerve block job you're entitled to get your operation you're entitled for the best chance of a pain free life i don't blame you in any way shape or form um but something has to be done here because this is crazy and people yes it's absolutely crazy because you know there's people that's waiting to see gp with something very simple which turns into something more serious because they're waiting weeks and weeks and weeks and then and turn that's making a casualty more that's making the consultants more busy you know it's just like i think there's a lot happening that people don't realize because somebody that could have a test infection could turn into pneumonia which is a hospital state you know it's silly little things like that the people that that we don't realize and it's just then we can't see gp okay all right it's very frustrating it's very frustrating i know and i can sense that um and i really a genuinely by the way i'm not just saying i really hope you get a resolution soon and you at least get on the pathway uh to give yourself a chance to get in pain free because you don't really have any guarantees that you're going to go through all of this and it will work but i pray it works and i hope uh things yeah well that's all right and thanks for yeah and i'm okay if there's something else you want to add if you want to is there anything else you want to add no i was just going to say that like i've come on here and i hope that if there's anybody that's listening today that's thanks they need to see the gp now it's the time to ring because you're going to be waiting weeks okay and all right thanks thank you very much indeed right let's take a quick break watch the show live now on youtube facebook and at highland radio dot com the nine till noon show with letter kenny credit union now offering mortgages from 40 000 to 600 000 euro with no hidden fees or transaction charges letter kenny credit union 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we're all in play your part at caringcommunitygames.ie craving a taste of bliss by the water the water's edge in wrath mullen join us from 10 a.m to 12 p.m daily and sample our delicious new breakfast menu we also have a daily lunch special from 12 p.m or why not sample our dinner menu from 4 p.m the water's edge wrath mullen where tranquility and good food come together choosing to have a chicken fillet roll is easy the hard part is butter or mayo plain or spicy coleslaw or cheese coleslaw and cheese shop smart feel good with centra's meal deal get a chicken fillet roll with two fillings select soft drinks and a small packet tato all for just 750 plus dr s deposit centra live every day now you're welcome back in uh on to or back to the program of beg your pardon we're welcoming on to the program uh leone rogers who is sister of patrice mccrossen of st johnson uh leone thank you so much for joining us i do appreciate it um we're also uh in studio with kevin shields from st johnson as well a family friend of patrice thanks so much for calling up kevin very much appreciated good morning greg um leone analysis not easy but maybe you might as much as you're comfortable with uh talk to us about patrice's condition and its uh discovery and progression uh well patrice was on well since about christmas time and she just couldn't get better so come the end of january she was like right i'm probably gonna have to go to the doctor so she decided on the friday morning to ring the doctors couldn't take her uh she was just really fatigued you know they end up she couldn't get up the stairs so she was like i must have a chest infection she's only 32 years of age yeah so this is very unusual for a 32 year old she had a baby seven months beforehand and she had an easy pregnancy or delivery and also she thought it's just athlete things but um so she went she got the nurse when the doctor couldn't take her and she went in and the nurse had says there she didn't like the color of her so she would rather go to the emergency department and we think it's a blessing she got the nurse that day because by the time she got to the hospital her hemoglobin was down to four so they put her in the major emergency and this was half four and they had her a bed by two that night patrice didn't settle stalls she there was abnormal abnormalities in her blood so they came in the next morning diagnosed her with leukemia hematology came in and that was her they were like we're getting a bed ready for you and doubling or in galway they were sending her they said you're going to be going for four months we've been in contact with them and she couldn't leave till she got her blood she had to get three units of blood before she left later can i so it was the sunday by the time then she went down to galway and that was her now this is just earth shattering news i mean how could any individual or a family prepare for that yeah like you're sort of going on it doesn't hurt you you're running on adrenaline and all like just for even the weeks after like for the first and and presumably part of the transfer to galway is to to examine more the condition yeah how severe it is what exactly it might be how might be treated so that's test after test after test how was that for patrice the first week really really difficult all the tests and the bone marrow biopsies and all like to see what kind of leukemia she had so this is then it was aml and that's the acute myeloid leukemia so it's on the blood and bone marrow but with myeloid it's really aggressive and it gets the cells when they're underdeveloped and it just attacks it takes over her whole body so then it was to see what kind of treatment they were going to go on with us like she went down there and she was 90 cells covering her body so they had to go on aggressive treatment so without an intervention at this point she probably wouldn't be with us now is is that i think if she didn't go to any on the friday whether hemoglobin at four all her abs was so low that she might have been there till the morning so it really it really from from when she how rapidly did it progress to the you know from where she felt you know there's something not right here to actually go into the ed how long a period of time was that well this was before christmas she felt unwell but i think then when the leukemia whenever the cells had changed or whatever that she was um this is it was a few weeks like she didn't see the symptoms but now that she knows about it she did have symptoms that she didn't see because it's not really what how we associate cancer is it really it's just it's just normally really really weak really really quickly yeah and as you say between a recent pregnancy and stresses and tribulations you put it down to other things yeah like she had a bad dose but she couldn't fight it off because her cells was she had no fighting cells like she's new to your clinic all the time with her condition so what is the the treatment pathway now what treatment she in what what treatment she is in her second cycle now at the minute her first cycle they went on give her an intense chemotherapy for like the first three days she would get three rounds chemo and then she go 20 days with two rounds a day it's really intense and then it's all the infections that come with it you know so by the end of that cycle they do her biopsies her bone marrow biopsies twice to see how it's going and they want to be able to get the cells onto remission but at the end of the first cycle after the aggressive treatment they didn't get her cells onto remission so that determined then that she needs the bone marrow now the bone marrow transplant it is like other things that we know medicine you require a match don't you yeah and those around her the likes of yourself aren't a match we're not about to go through that process as well genetic test that was done with just the blood drawn but um none of us was a match we were hopeful that maybe it's out of four of us like the one of us would be so it's fine near a match now but we have to see she's probably on now at the minute getting another biopsy the bone marrow to see how the second cycle has went how the cells is going but like it's the infections and all the come with it like you know she's been lying she's not too bad there at the weekend but the weekend before like they were treating her under ICU guide so in palliative care and okay you know it's it's a really tough road for her yeah and they hope now in the next eight to ten weeks that they would get a donor finder donor so she's a battler but she has a battle on her hands but there's no doubt she's a strong woman she's very strong um in terms of seeking out a a suitable donor I don't know how that works unfortunately sorry um is there like a panel or people have been in contact that they want to get on the register you can't go on the register until you're a blood donor so the way that they want people to check online to see if they're eligible to be a blood donor and when they go to the clinic the clinic sorts them to get on the register is there any indicators that someone might be a match for her nothing geographical relation or nothing like that there it's just like it just so happens that another person would go on the register and you go on to a worldwide database okay so they will see from there just they go through that like you're sitting there waiting that hoping just the right person here's something like this the right person is a blood donor it becomes one the right person registers as a as a yeah to try and find a match yeah okay because this is the only thing like the last time when they see the sales couldn't go into remission as she's been told like she could go through these treatments but it's going to come back they can't save her so this is her chance to save her life yeah and Leonie how how is her young family and and all coping I mean her children are obviously they're under the care of the family and Gary and what have you but they're so they're so young and her being apart from the children probably is almost as difficult as everything else she's going through not easy at all not easy for any of them um like Gary like to be two places at once so just trying to balance it all with all of us um the winds are like a three year old says one day the hairdresser was uncutting their hair and he's like do you want to stay and look after me because my mum is away they just he doesn't really understand but the bigger ones he knows his mum is going but the bigger ones then like her seven year old I took her down at the weekend for we better one do one and she lies up with Patrice when she's hooked up and she's like you know mommy why does this have to happen to your family and Patrice is like there's no answers he can't how do you answer them that you can't you can't it's absolutely impossible because you don't you don't you you want the time with them but then you have to yeah Kevin also friends answer Leona you by the way you've done amazing it's very very difficult for you too and you've done absolutely fantastic in just helping us understand Kevin I mean who couldn't be moved by this story I think particularly as horrible what Patrice is going through but then when you think about the children I think that gets everyone on a different level as well on top of what we're already feeling this is obviously it's the family that's affected it's the friends and the community too this is just a big shock for everybody yeah it was a big shock now when I came out at the starting all and being the family of Patrice and Gary is and the Duddy family and Rogers family everybody was pulling together so I was down in Galway at a rally and I met Wally Rogers Patrice's brother and had a talk with him down there about Patrice so when I come back to Dunny Gawlyn and the Monday after the rally I had to think about it and I spoke to my wife Vanilla about it and then on Tuesday I called Declan Boyle of Sonny McSweeney's McCafferty Group and said to Declan what I was thinking maybe running a fundraiser to help the family because I knew there was going to be a tough road for him and expensive road accommodations and stuff and all so Declan told me yeah definitely go ahead and whatever back in our need he would be there and so he set up a meeting with Phillip the manager and Mark Emma and Shannon so we agreed with Wally it was okay with a family to help this fundraiser so I started off to do something I thought was going to be small and the first person I phoned was Donna Kelly about it and Donna right away took whole play tickets valued at a thousand euros and I'll give you four spud at a thousand euros full VIP and so it started the ball rolling like and since then everybody has been fantastic towards the fundraiser like and you know I went outside then like to talk to different people outside myself and Sonny's desk so I spoke to David Fisher from Oakfield and Niall McGonigal from Iain Motorsports about a bit of advice on it and they all give me great advice so I've been working very closely with Declan and his team and to say we have got serious serious donations and this auction is taking place on Friday the 19th at 7 p.m in Sonny McSwine's in Lederkan Eats in aid of Patrice Macrossen and I suppose Kevin you know you can you feel as a community your friends or whatever you can feel helpless in this situation you know we wish we we you wish you could take it all away but you can't but what can you do is you can try and eliminate other worries like the pressures in life so that Patrice and Gary and the family can focus on getting her better and that's what this is all about isn't it really is to support them yes definitely support for the family because as you said Greg it's very hard in the family what they're going through so they don't need that extra pressure of you know funding to keep them down and go away and up and doubling and all so what to support not just to fund them but knowing that the support of the people has behind them is just unreal what we're getting like and listen some of the lots are hitting on stuff that's hugely popular up here of course you mentioned the VIP Coldplay tickets worth over a grand rally seats from the top 10 rally cars in Ireland huge our own Jason Quigley world title contender a framed boxing gloves of his that would be a great thing to have that and so much more besides I think you've been really pleased with the generosity of of those that you've approached that you haven't you haven't left you wanting everybody I've approached has been unreal like you know as I said that the first S was done a Kelly Denver full boxing club gloves they're off the wall give it to me in the frame Callum divine a phone Callum no bothers sir that was his answer whatever you need let me know so they have all given seats even when I was in bed last night I got a message from Matt Edwards who's from Wales who's given a seat out as well and you know it's unreal like you know but not alone just a rally and dealer sports like soccer Gaelic from Brian McCormick and Michael Murphy like and there's too much to mention but there is a massive list of brilliant prizes brilliant lots for this auction and it is in McSwain McSwine's letter Kenny in aid of Patrice McCross and and I suppose you know the only one we're going through difficult times we look at little things to sort of help us get us through the reaction from the the local community but even more wider than that backing Patrice and her battle and the family as a whole as well we can't believe it like we just can't believe the generosity and the support from everyone like they told Patrice and there that she says I'm leaving you some my hands to get me back up the road and they said you need loads of prayers too and everybody's been rallying about praying and all like Patrice didn't want to accept anything she was like no some other creditor needs it and now is the roads getting tougher we see like we don't know what's going to be six months or a year from now like we just yeah okay this is a reminder to the importance of donations in terms of blood donations which of course Patrice has had to rely on get yourself on the register too for donating organs or in this case bone marrow bone marrow get on how do you find out more information about that on their website and at the blood donation clinics we have one on download tonight and there's two in Balboffay the next two nights as well for people that do want to go to make an appointment or anything but at this stage her her whatever makes up her bone marrow has been tested against an international database at this point is it everybody that goes on the register it's not just Ireland it goes on a worldwide one so you could save somebody in Spain or whatever in the situation listen um go ahead please Kevin yeah just one more thing Greg um Sonny McSwine's and McCaffrey's has set up a GoFundMe page for people who can't attend the fundraising event on Friday night and anybody that donates on the GoFundMe page they've been with a chance of one on one of five prizes as well okay just to help people who can't make it on the night brilliant stuff okay listen fair play yes go ahead Leonie do you want to say something okay well done you did brilliant Leonie Rogers sister of Patrice McCrossen of St. Johnston Kevin Shields also from St. Johnston and a family friend of Patrice McCrossen if you can get along to that event on Friday night and where can people find that website do you find it on Sonny McSwine's social brilliant okay right we'll link that of course in our socials thank you so much both of you The Ninetyl Noon Show is brought to you by Letter Kenny Credit Union digital loans now available apply online or via our app today and get your loan transferred directly to your current account the Donegal branch of the Irish Kidney Association are hosting a service of remembrance and thanksgiving on Sunday 21st of April at 3 p.m in St. Mary's Church John Ardour to remember Argan donors and donor families Argan donor awareness week runs from 20th of April to 27th of April for more information visit aka.ie ready to feast your ears on this sizzle of success Arlan's most awarded specially selected stake range from Aldi 100 percent Irish board be a quality assured strip loin for two for just $9.99 or how about dry aged black Angus ribeye the best ribeye in Ireland two for just $13.99 sounds good taste even better go all Aldi while stocks last FBD doesn't stand for fighting lad dentists ferociously beating drums or feline business directors FBD stands for support we support business owners in Ireland with tailor-made services from personally assigned advisers visit your local branch to talk to your FBD insurance team FBD insurance support it's what we do underwritten by FBD insurance plc FBD 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Ballet Buffet okay students at St Union's College Letter Kenny are currently running a production of the outsider is adapted from SC Hinton's classic novel it's on the 24th and 25th of April in on Green on Theatre Letter Kenny Tommy McIntyre is an English teacher in St Union's College Letter Kenny is going to frame this for us before we chat to some of those that are in studio with us Tommy so talk to me about the this show okay so the show was taking place on the 24th of April at 8 p.m. and on Green in Theatre and it's based on SC Hinton's novel The Outsiders written in the 1960s by Susan Hinton okay she was 16 years of age when she wrote this novel and it's selling half a million copies every year so we decided we had a drama club there and we decided then to actually you know why not do our own adaptation of the the novel okay and has this it's been done before you had uh did you have a script to work off or was it adapted or what's the story pod migrants no we uh we adapted the novel uh ourselves for the stage yes so there's there are a few small changes towards the end but they don't actually uh infringe on the and the story itself talks also a little bit about the story of the outsiders okay so it's set in 1960s Tulsa Oklahoma it's about a group of uh young men like these men standing here and ranging from 14 years of age up to 20 and they're called the greasers they're a close knit bunch again like these guys here and they have rivals called the socials and they're the rivals in social class so the greasers come from the less well off area and the socials come from kind of the better area and so yeah it's about their adventures together yeah and um great group of people to work with presumably oh absolutely fantastic yeah but there's a lot of work a lot of work involved in getting this right yeah oh yeah definitely a commitment absolutely a lot of commitment and uh Rachel uh Rachel McShane and Dianne Elis and Fiona McDade have been absolutely fantastic Rachel directs us along with Dianne Elis and Fiona does a lot of the the staging and the props yeah and um was there recent awards one yes they took place uh that the Ballard Theatre uh drama competition took place down in valuable february and uh we were fortunate enough uh to win four awards there including um Matthew uh as best actor there so yeah all right brilliant stuff and was that a performance of this or something else it was a 15 minute uh section of it yeah brilliant so and so you managed to win over the judges in 15 minutes people are going to get a real treat then with the full show uh in a brilliant setting on the 24th and 25th of april and on green on tickets obviously there's going to be huge demand from from parents and schools uh others within the school but tickets available currently are they are tickets available now they are they are flying out the door quite quickly now so they always are yeah i know so so yeah so we all go well yeah we are last minute dot com in this canteen region so if you want to get along to this on the 24th and 25th of april and on green and i suggest you contact the box office immediately right so what are we going to hear now um tommy you're going to hear the opening uh two or three minutes uh of the first act first scene where uh pony boy has just come out of the cinema he's just watched uh paul newman film and he's about to have his first encounter with the the saucers done done done right okay lads whenever he's already take it away and move this over here slightly there do you want to move scooch in that way a bit all right go ahead right curtain on i've always liked paul newman he looks so tough you need to be tough what i'm from even walking down around the east side our territory you really can't loan it greasers can't walk alone too much or they get jumped or someone drive by and shout greaser but i just don't like going to movies of anyone some people talk too much and i kind of want to get into the movie and live the actors it makes me i don't know kind of uncomfortable having people there like someone looking with my shoulder when i'm reading the book well that would not happen in my house anyways soda my second older brother would not crack a book if his life depend on it and darryl who ever calls darry well he just works too hard to be into them or me for that matter greaser i i really used to be walking alone darryl would get mad real mad if he knew i'm supposed to be a smart one he says making good grades and having a high IQ well i guess he's just trying to be the grown-up in the house ever since mama dad died in a car crash her gang the greasers well we steal things and drive old slipped up cars and even have a gang fighting on again hey grease well you do not hear all alone grease ball leave me alone why grease we just want to talk shoot the breeze with you and maybe just maybe give you a nice haircut what are you crazy hey grease how'd you like that haircut to start just below the chin darry soda i'll shut him up for Pete's sake darryl darryl shut it grease hold it someone's coming see you're on grease ball that haircut's gonna have to wait you okay pony i'm good typical socius i'll talk damn socius this this this is our territory come on to a bit are you sure you're okay pony boy i'm good johnny really just got scared as all you all right pony boy okay darry honest quit shaking me sorry they don't hurt you too bad did they you got caught a bit pony boy you're bleeding like a stuck pig they pull a blade on you yeah nah they give us a slip but dirty yeah the kids okay he suffered than he looks i thought you were still in jail darryl nah good luck for good behavior nice bruise you got there pony thanks why are you out here on your lunchtime anyway kid i just didn't think shit ain't brilliant lads well done give yourselves a round of applause unfortunately there's no one else here to clap okay that is just a sample and for those of you listening already there's many different voices in there as well so we've got one two three four five six seven of those taken to the stage on the 24th and 25th of April in non-green on theater and it is their production of the outsiders tickets are available now but are going out the door quick so if you want to go and watch that you need to get on to the box office thank you everyone really appreciate it well done teachers great to have you in that was something different something very enjoyable all right so we'll be back with you on the ninth loon show again tomorrow morning from nine


That was good Friday and popping. I am so excited, what have you planned for先生?emitism of迎D ICH answered that I am very excited. But what we are waiting to see will be even better in countries with Nov Hear. From you, you said. When are you taking down this heart, Where are you leading us? We can name a rafu Ya ni kwanzi asaim baka Monday I don't know what to do I don't know what to say But if you have blood kindly hello to me On Facebook I go by the names Karen Kairetu And on Instagram I'm called Karen Kang And on Twitter ni Karen Kang One one Anyway guys we are interviewing The biggest artist in Kenya right now Of course Because there are lyrics They are good and vibe me sexy So come kawahida hip-hop Thursday To naleta hip-hop artists And I have the one and only Rahim Guys if you thought Sidriati, muslim and hip-hop couldn't blend Now you know this is the man Insha Allah First of all what does insha Allah mean? Insha Allah means Kata patana Mipangwa maulana So jotu jikata patana So majaliwa means insha Allah All right, yeza Mazi wunakam say miyang Wunakabado steel wujapitishang Wumyakaflani ya wuze So I want to find out from you How old are you and when did you start doing goma?  Feels good you know It's pressure All right So wuna representum ta gani Kusapa wa itu fei fo We are all over Kenya First of all just so you know We are all over Kenya And personally I'm representing Dargoreti Kona And we have another representative I mean kaa Yo forget that I mean we had another representative Over here from Dargo But where do you come from? I come from Kawanguari 46 46? Dargo is massive Massive So how is it Being an artist for Kawanguari Making it in Nairobi Yeah it's challenging It's a bit challenging Ki aje?  Maybe you can Tambulisha him maybe Complete accident murderers Accident murderer I love that song But anyway What's your latest track The one that you just released You told me it's a love song Yeah it's a love song We did it Why romance La biluwa sayapa matzaluwa nachkwa shoka My friend My fans My fans shulana ni pressure Ni otole love song So we decided to write a love song We did it to Ketlin Yeah in Tata How was it recording Yeah With females It was nice It was nice It feels nice I always feel like you Male rappers Miki leto A female rapper You always feel threatened At some sorts As long as you're working together And the content is nice Look at the scenario Your calligraph and What's her name?


Everybody forced here rip in 2022 certified football hobby 4 box number 10 random team style You can find these results at latent sports cards comm. Thanks for joining everybody and good luck here. We go Sup Rick So you guys 16 to go on the mixer just about halfway full Super close to halfway.  It's breaking about an hour or so a little over an hour Again, it's got eminence 2018 prism. You got 21 select 2019 contenders 2022 Nt collegiate first off the line and hobby. I'm a collegiate first off the line and hobby as well Starting off with a drew lock Seahawks 13 out of 50 dark horse is Giovante Gold team Kyler for the Cardinals numbered 31 of 75 Phillies for sure Rick. I think I would I would argue I Would argue that every single fan of The of that isn't an AL fan So if you're not a Yankees fan or actress fan ever moves on you're going for the Phillies I would bet I would bet we got David Bell RPA for the Browns 150 to 199 Taylor we got Johan Dotson rookie commanders 29 of 275 Got Raiders Derek Carr numbered 147 of 149 Gold team Matt Ryan Colts Red Sox and twins fan. I'm a Red Sox fan. I Don't hate the ashows like people do but I preferred them not to win the world series Ricky Jersey Packers Christian Watson 288 of 399 D hop there God Packers Ricky Otto quay Walker numbered 72 of 99 Certified stars Russ Wilson. Yeah, excuse me Trevor Lawrence Jags numbered 293 of 349 299 290 290 of 349. What the heck am I reading Kirk Cousins gold team?  Oh, oh Right behind the Brady. I mean Bailey Tyler Algear 97 to 275 rookie Falcons Did you just say the Warriors have 89 points at halftime? Oh My god, dude bangles Joe burrow 154 of 199 that team's pretty good, huh? Mac Jones certified stars 89 points halftime We're also not playing any defense to be fair though if allowed 71 rookie Jersey commanders Johan Dotson to 14 of 399 That's more points than the C hot than the then the Broncos are gonna score all season the Vonte Wyatt Packers rookie Otto number 97 of 149 graffiti breeze hall Jets certified stars cup 16 to go in the mixer guys is get it filled up Does sound like an all-star game Raiders Josh Jacobs had a big day today three of 75 The re rushing touchdowns a lot of yards to Alec Pierce rookie Colts 164 of 325 Kyler Gold team Guy rookie patch not rookie but patch card Daniel Jones 23 to 25 Giants nice to color there Carson strong rookie graffiti Eagles 68 of 199. I don't hate the Astros At first I did but I mean I blame Rob Banford for not holding them accountable Kenny Pickett on the rookie Jersey Steelers 166 of 199 Gold team Derek Henry Derek Henry numbered 158 of one of 199 158 of 199 A little bit of foil missing there all I know is go Phillies George Pickens graffiti Steelers rookie Carson strong Eagles rookie patch Otto 63 of 149 Got a Roger McCrary rookie 129 to 399 for the Titans Eli Mitchell Niners numbered 312 of 349 got gold team Debo Niners 5 out of 99 miners offense looks really good today, huh? Their new weapon. I'm just kidding guys. I know it's like one day of practice Malik Willis 108 of 249 rookie Titans patch card How to they wasn't a main culprit though. It's like Bregman and Springer and some others I say Kirk Cousins gold team Otto 4 out of 10. There you go Vikings Caffrey seal of approval who is 11 on the Seahawks Miles Garrett for the Browns 2 out of 75 God graffiti James and Williams Lions rookie Malik Willis rookie Titans. There we go 195 at 325 Yeah, I think reports have come out since that says Altuve wasn't really involved with it CD lamb, but a lot of the guy. I don't know. It's very weird like not the players weren't punished It's weird that Springer just left and like nobody cares Like nobody cares. Oh, yeah, Springer a good guy. He's on the blue jays Adam Thielen 12 out of 149 for the Vikings AJ Brown seal of approval Eagles numbered 68 of 149 that's impossible to read. There you go Eagles. Try to read that number Ravens rookie Jersey Kyle Hamilton 154 249 88. Yeah with the with the logo right there. I couldn't really tell I Know what it's out of it. Yeah, I know what it's out of her. All right mixer is at 15 to go We are halfway full half way there. Here's a Lincoln chat for you. Yeah, I agree with that Mike Billy Joe Dupree Cowboys Otto number 22 out of 25 Yeah, they'd be silly to at least not give him like Every opportunity to win that job and spring training like if he looks even decent they should just play him Like if he's hitting over 200 and playing fine defense, they should just play him Lions Jared Goff 256 of 275 Rashad white rookie bucks 105 of 199 dark horses etn 80 zappy on the rookie jersey 180 of 249. I'm having so much trouble reading the numbers on these cards I think it's all like the foil on them Bo Melton rookie nine to ten for the seahawks Nice bow bow nose easy e on the rpa dolphins 206 of 249 erikizu kanma Nice easy e4 color rpa commanders ricky jahan dots and graffiti And James cook rookie for the bills 287 Of 325 there you go bills and that's gonna do it guys not gonna recap running run a little slim on time here for the next break A little behind actually so thanks again everybody pretty short break though more football coming up


Hi, welcome back to the fourth class of metals in biology. I hope you guys are studying all of you from the books available and some of the references that we have given during our lecture. One of the book that we are following is Principles of Bioinorganic Chemistry by Lippard and Berg any other bioinorganic chemistry book another exciting one would be the one by Professor Keim. So, please study from any book if possible try to study from these two books. In any case we were in the last class we are trying to discuss metal regulation and gene expression. Now, there are consequences as we are mentioning that the iron metal iron or metal iron concentration at very high level or at a very low level will have its implication in our health. So, for example, there is iron regulatory proteins which controls both ferritin which is a iron storage protein as well as transferrin which we were discussing in the last class translation. So, iron regulatory proteins IRPs in short they control both the ferritin and transferrin translation that is quite interesting right. Well by this way they can control the delivery and storage of the given metal iron such as iron as I was saying for iron regulatory protein. Regulation of toxic metal ions are essentially very important body ensures that no toxic metal ion even if it is internalized stage in the body for long. So, there will be a process by which toxic metal ion will be excreted or will get rid of those such as such toxic metal ion such as mercury will be thrown out of the body. So, that sort of regulation is also present. Well zinc finger protein overall controls the transcripts and process as I was mentioning IRPs controls the ferritin and transferrin translation we will see also in the subs in this class that calcium 2 plus which is a secondary messenger and found at the centriole of at the synapse the will also be important in controlling different metal ion concentration. Today we are going to first discuss the regulation of iron levels in cell. Regulation also has to do with the storage as I mentioned the storage protein for iron is ferritin the protein name is ferritin. It can store a huge number of iron centers while iron storage protein has 24 subunits and each of them are having 175 amino acid approximately. It has cubic symmetry apopheritin means minus the iron center of the ferritin where no iron center is bound apopheritin can house 1000 iron atoms in the central core. A ferroxidase center usually loads the iron into the apopheritin. So, there is a protein ferroxidase which is involved in loading the iron into the apopheritin so that ferritin can store the iron center. So, this is something like a relay mechanism. So, ferroxidase catches the iron and then it delivers to the ferritin. So, the transferrin the uptake protein that we have discussed separately ferroxidase is the transfer protein. Ferritin keeps and releases to ferroxidase and ferroxidase is loads it into the ferritin. Now, metalloregulation as we were talking in bacteria it occurs at the transcription level. So, DNA to mRNA formation at transcription level in bacteria these metalloregulations are occurring. In mammals the synthesis of apopheritin and of the transferrin receptors are regulated at the level of translation remind you not at the level of transcription. During mRNA to protein formation that is the translation step in mammals the synthesis of apopheritin and of the transferrin receptors are regulated. Now, as we know these iron ferritin subunits are going to have a quite complex structure all these exact structure although some crystal structure are known, but it is you know nailing down the 1000 iron loaded irons a ferritin center is not very easy. Of course, apopheritin structure are known which is which is having a 3-fold channel and also 4-fold channel overall there are many subunit as we have discussed and it can load the iron center in the ferritin. Now, let us look at briefly the ferroxidase centers which is known the crystal structure is known. Let us look at the apopheroxidase where you see over here this is the apopheroxidase structure these the site A and B this is taken from this book A and B can load or can house 2 different iron center in this site. This is the iron loaded form on the top as you can see the glutamate is bridging between these 2 iron center this is ferroxidase remind you ferroxidase loads iron center into the ferritin which is a storage protein. Now, this is capable of binding iron center this ferroxidase you see one histidine one glutamate another bridged glutamate water molecule another glutamate over there these are hydrogen bonded with tyrosine and even this water molecule as you may know will also be hydrogen bonded overall it is a huge network essentially it tells you that in ferroxidase 2 iron center can be incorporated and how their structure is going to look like this gives you a clear idea. Of course, ferroxidase is not ferritin ferritin is the storage protein ferroxidase is the one which is helping in transporting in a way of course, transporter is essentially the transferrin, but overall from transferrin ferroxidase is helping in loading the ferritin. Now, ferritin structure at a very simplified iron binding site is not really a good site what we have we are we can see for example, is the one where we have these you know synthesis attempt this is a science paper in 1993 which clearly shows that how perhaps multiple iron center can form a nice structure a mixed valent poly iron oxo cluster you know as this can be a model of ferritin core and this is somewhat perhaps the ferritin would look like of course, this is a model core model structure which shows that how perhaps ferritin might will look like in the in the in the ferritin ok. So, a lot of iron loaded sites are seen in this see these are mixed valent poly iron oxo clusters. Well in the metallo regulation then we have learned that in bacteria a single protein that is for iron uptake regulator in abbreviation called FAR FUR a single protein that is FUR FAR controls the transcriptions of genes involved in psi dero 4 biosynthesis ok. As we have mentioned that in bacteria the control material regulation happens at the transcription level and that is at that level the psi dero 4 which we are discussing in the last class controls its biosynthesis is controlled ok. Psi dero 4 biosynthesis is controlled therefore, iron uptake is also controlled at the transcription level. The protein that is involved in this process in bacteria is FUR for iron uptake regulator. FAR is a dimer with subunits something like 17 kilo Dalton at high iron levels the FAR protein has bound metal and interacts specifically with DNA refreshing the transcription of course. So, FUR protein the FAR protein has very high levels of iron. This FAR iron protein has metal bound in them and they will interact with the DNA specifically and therefore, these you know transcription can be controlled or replaced. So, overall what is happening is this FAR protein in FUR FAR protein in bacteria it is loaded with metal it prevents the DNA to RNA formation unless it is necessary. So, the high metal ion concentration of FAR will control the transcription or will repress the transcription. In bacteria that is how metallo regulation is happening and that is how the psi dero 4 biosynthesis is getting controlled. If psi dero 4 biosynthesis is getting controlled let us say then iron uptake will not be uncontrolled will also have control on the iron uptake. In mammals expression of ferritin and the transferring ferritin is the storage protein transferrin is the transferring protein which is which is involved in transporting iron as well as ferroxidase. So, expression of ferritin and the transferrin receptor is regulated at the translation level. So, overall as you can see there is lot of checks and balance which controls the metal ion transport when the metal ion has to be transported inside the cell that gets controlled by other protein either at the transcription level or at the translation level. In mammals it is at the translational level where it gets controlled at bacteria the biosynthesis of different psi dero 4 which is responsible for transporting a metal center let us say iron their control is happening at the transcription level. Now, we will discuss little more on these topic and we will come back soon. So, we were discussing the metallo regulation metal it is important to control to be controlled also metal plays a vital role in folding of biopolymers. Let us now try to see the metal folding of biopolymers the role of metal in folding the biopolymers. So, biopolymers such as protein as you know metal can bind with protein at different sites they are polypeptide proteins right. Now, this peptide has amino acids. So, the amino acid residue can bind with the metal ion and therefore, the structure of the protein can be modulated by a given metal ion. Let us say you have a very high coordination number containing metal ion. It will fold the metal protein differently compared to the low coordination one. Let us say you have a metal ion which can coordinate with the different side chain in an tetrahedral fashion or in an octahedral fashion or in a square planar fashion depending on that we can pretty much say that metal ions can fold biopolymers differently. Different metals can fold biopolymers differently depending on the metal ions its charge size as well as the coordination number the biopolymers can be folded differently. So, it is essentially what we see that if these metal ions are not the right one or excess of metal ions are present or less of metal ions are present the folding of biopolymers will change and therefore, the change of this folding pattern can also lead to the agglomeration or precipitation of the biopolymer and therefore, some of the disease such as you know Alzheimer's disease and Parkinson's disease can be directly be associated with the adventurous chemistry simple chemistry coordination chemistry that this metal ion can play. Let us look at the few principles for metal folding in case of the biopolymers. Well metal ions simply can act as template to organize the 3D structure of biomolecules that is what we were discussing now right. It can act as template for the 3D structure of biomolecules. Now depending on the necessity metal ion can lose water molecule when binding to proteins. Let us say you have a hexa aqua metal ions of course, no metal ions are completely free it has aqua molecule. If need be only 3 of them or 4 of the water molecule can be chopped off or can be dissociated from the metal center and the protein can come out. Selectively any given number of water molecule can be lost from the metal side and then the binding of the protein can therefore, be affected by these loss depending on the proteins we can have different orientation with respect to the metal ions. Metal ions can retain many water molecule can lose many water molecule and also can retain many water molecule when binding to nucleic acid and therefore, these are the processes which will also affect the hydrogen bonding right. Metal ions binding can also facilitate interaction between biopolymers. Let us say two biopolymers are there they are not interacting with each other at all a metal ion in between can act as a communicator or some other side reaction other you know unwanted things can also happen. So, two biopolymers multiple biopolymers can be interlinked through the metal center. It is a center of attraction for the biopolymers. Biopolymers feels attracted towards the metal ion because metal ion can bind with them right in a coordination atmosphere. Metal ions with large ionic radii utilize high coordination number for its function right. Metal ions with low size or low ionic radii will usually have low coordination number for its function. As you can see metal can interact with the polymer biopolymer. Therefore, it has also the ability to induce protein misfolding which may be relevant as we mentioned for different human diseases. So, overall we will also try to discuss let us say at least one thing for sure the calcium binding proteins and their EF hand domains in a moment. And also we will see if we would like to discuss the metal mediated protein misfolding and the different disease states. Overall this metal binding is crucial in case of conformational changes that can happen in the protein metal binding and this can lead to the biological function. Well we will be definitely discussing this in detail. Let me tell you that protein folding is quite important to understand. We must see how do peptides are folding and proteins are folding. Now imagine that we have a situation where only one bed is there and one person is sleeping. Then therefore, the person can orient himself or herself the way he or she wants this is a very relaxed situation. But on the same bed if 10 people has to sleep now they will feel like disturbed and therefore, the orientation of their sleeping mode will be completely different. So, in a crowded atmosphere versus a non-crowded atmosphere the protein will fold differently. But more importantly when we have a metal ions incorporated into the crowded atmosphere of the protein which is usually the case in a crowded atmosphere versus a non-crowded atmosphere these metal ions will also play key role in folding the protein. So, inside the crowded environment of the cell this metal ion can play a very crucial role in folding the protein. This folded protein can give rise to the new conformation to the peptides and protein. As I mentioned metal ions can mediate protein folding which can have physiological and pathological consequences. Let us look at one interesting example of the calcium binding with a protein or which is known as calmodulin or calcium binding protein. So, this is the calcium binding protein, this is the apostate of cam or calcium modulating protein and this is the hollow cam. This calmodulin which is calcium modulating protein can consist of usually consists of 149 amino acid monomer and it is expressed in all eukaryotic cell. And overall if you look at overall up to 1 percent of total protein mass binds 4 calcium ions which is quite exciting 1 percent of all protein mass that we have all the protein that we have 1 percent of that binds with 4 calcium ion. The binding constant is quite good and the dissociation constant is of course, of course, not that high right. So, this overall calcium binding tendency to the cam or calcium modulating protein is going to give rise to the very order conformation. Let us say without calcium it was completely disorganized with calcium binding as you can see it gets organized in a particular fashion right. So, calcium to plus on the apostate of calcium modulating protein can gives rise to a open hollow cam structure. This is a closed structure this is not useful for lot of function, but in presence of calcium we have a very highly structured situation which is essentially can do quite exciting chemistry and the biological function. Now, we can try to look at these domain separately and try to see what is there in this organized structure. Where is the calcium? Calcium 2 calcium are here 2 calcium are there. Now, these are EF hand domain and these are having many crystal structure and you know first crystal structure was obtained with parvalbumin. Now, this helix loop again helix this structure is quite interesting and quite unique for the calcium binding and the calcium this cam protein right. This helix loop and the helix structure is quite interesting and we can if we can zoom into that we find that there are 9 residue into it and EF hand domains almost always occurs in pairs. Let us look at some of the more closer structure of it. So, this is helix E right and this is helix F that was in the yellow and the blue fragment and as you have seen the calcium is binding over here. So, the helix loop helix structure this helix loop helix structure are quite essentially a very organized structure that is controlled by the calcium binding. This EF hand domain almost always occurs in pairs. As you can clearly see the calcium is bound in this loop you can see in the right hand motif this is the helix E, this is helix F and the calcium binding happening over there. If you zoom on to this site more carefully the calcium binding with the protein backbone can be seen very explicitly. The primary coordination sphere of calcium modulating protein cam is looking like this it has 7 coordinated calcium site. So, this calcium is 7 coordinated we have aspartate, we have main chain and we have another aspartate, we have aspartate this is protein main chain, this is glutamate, this is water molecule, water aspartate, main chain aspartate, glutamate and aspartate. Overall this gives rise to a situation where the primary coordination sphere of calcium is, calcium is 7 coordinated and this is the crystal structure very clearly showing in how in calmodulin we have a clear calcium binding. Well there are many factors that influence calcium coordination to EF hand domain. One of them is certainly the cooperativity and another interesting issue is the cellular magnesium that also controls the calcium coordination to EF hand domain. After binding with calcium this cam protein now is ready to bind with different cellular targets and can promote exciting chemistry. Today we will stop here and in the next class we will show how calcium bound protein that is cam calcium modulating protein when it is bound with calcium can affect a series of events and that is why perhaps it is called as a messenger protein. It sends the message to do a particular activity or it activates a particular protein by binding with calcium in the cam right. Of course, KD values of EF hand domains of calcium and vary usually from 10 to the power minus 9 to 10 to the power minus 4 molar and there are many clear correlation between calcium affinity and variation in EF hand domains let us not discuss too much into that, but overall what we have seen that the calcium in particular in the last part of this class can bind with the protein and these are found a lot in our body. Overall 1 percent of the protein mass binds with 4 per 4 calcium center that is quite phenomenal and how it is bound or how it is binding is quite exciting as you have seen the EF hand domain motif where calcium is really bound tightly in the helix loop helix fashion and the coordination environment is quite crystal clear and has 7 coordination with the calcium. The effect of this calcium binding to cam and how it triggers a series of events will be discussed in that next class. Please keep studying, we will come back soon. Thank you very much.


Greenline comes on now, you can see it, takes her away and we go to the first turn and it's all about Madsson on the inside, Madsson Janowski there alongside him, Madsson makes half a mistake, Janowski now pounces, brilliant move from the Polish rider. And Janowski from Gate 4 had made a good one, he was in the perfect position to take full advantage, you can see there, Madsson on the outside, he knows at that point that they're all coming through, luckily for him he manages just to get the bike. Five, Greenline is on, where'd they go? Inside his work well, Woopendon's there though, look at that from Smalsnake hugging that inside, he now comes off the corner, Woopendon's there, Woopendon's there right alongside him, terrific effort from Ty Woopendon. You can see this again, this is a superb move from Woopendon. Yeah, Woopendon puts himself in a great position here to make a charge down the back straight, looks like Smalsnake's done enough, he's getting a little bit of a wheel spin on the freshly water track on the inside. Number 10, Greenline is on, tapes are up and away they go, Vasilek's made a really good start on the inside, hugs that inside. We've got Mikkel Mikkelsen right out wide but here comes Max Frick between the riders here, getting the better of Robert Lambert as well. Great response from Martin Vasilek off the inside, makes a great jump, hits the first turn in front, body language there, he's right on the front of the seat so when he hits that rut. Keeney looking for that Greenline, now it's on, tapes are up and away we go, Uli's there, Uli's made a really good start. Martin Vasilek though, that run to the inside, helps him out, Uli round the outside, speed personified, brilliant stuff, fires himself into the front. Whooping for joy in the pits, we're down, Uli. He's just having fun and he's the first race winner from Gate 3, doesn't make the start, that goes down to Martin Vasilek but he just keeps himself... Also, here we go then, Greenline's on now, tapes are up, Jarvis on the way they go, Dudek's made a good start from the inside. Hugs that first turn, there's Barca Smasher coming through in the second place and Freddie Lingwin also getting the better of Robert Lambert down the back straight on the opening lane. Yeah, from the inside, Patrick Dudek, been electric from the start all night long, not necessarily known for his starting but he's hardly put a foot wrong all night long and Smasilek just settled into the race in second place. I honestly don't think, A, he had the speed to take the chase to Dudek or necessarily he wanted to, I don't think he wanted to take the risk. I think he's very, very happy with 18 points but that man there looking back on the opposition, it's a relief for him. Back to form, back to his best and a ground free win.


Thank you very much Thank you all very much and Welcome to the White House Somebody once stopped Louis Armstrong on the street in New York City and asked him how to get to Carnegie Hall Practice he said practice practice practice Well, that's how each of you got to the Olympics and I must tell you we have hundreds of groups here each year But I can't think of any that I'm prouder of than you Years of practice and years of dedication Years of dreaming and working for your dreams have made each one of you metal or no metal an American champion And an American success story America cheered when Bonnie Blair Won her gold and bronze medals setting a world record in the 500 meters When she was asked how she did it Bonnie said I think I just got it on guts One thing I noticed on TV when Bonnie won that Blair bunch Bonnie's family as well as her friends from the Champaign Illinois Police Department cheering her on I Have a hunch that love and support of both family and community helped Bonnie find those guts While we're on gold Brian Boytano Is there anyone who soared the way you did in your gold medal-winning long program? Eric flame flew as he captured one silver in the 1500 meters and just missed three bronzes and Jill Watson and Peter opacard You were a thrill to watch as you captured your bronze medal And like Bonnie each of you has had that backing of family and community over the years And that backing has given you the strength to compete with grace and sportsmanship Debbie Thomas Sometimes when I look at everything you've done and are doing Olympic skater pre-med student. I wonder if your name should be Debbie or superwoman I know that you draw a lot of your strength from the Strength and love and example of your mother Yes, you were disappointed with your bronze But the grace with which you accepted your disappointment Won you a gold medal in the heart of every American talking about gold medals from America's heart There's one that goes to Dan Jansen for his courage and one that goes to his family, too their devotion to each other Captured the heart and earned the admiration of our entire land Dan you receive the u.s. Olympic committee's Olympic spirit award and everyone here and around the country Applauds the committee's choice You dedicated that honor to your sister her memory and your entire family And now you're back in competition in number one in world cup points Yes, your family is very precious in your life And if recently a cause of great sadness It is also a source of great strength and that strength is an inspiration to the entire world Now I won't forget the demonstration events freestyle skiing Gold in the aerials Melanie Palinick and my book anyone who even tries freestyle aerial skiing deserves a medal And Silvers in men's and women's ballet Lane Spina and Jan Boer Finally in disabled skiing skiing. We had a sweep in the women's modified giant slalom Diana Golden Kathy Gentile Silver that was gold for Diana Golden. I had left out one of the goals didn't I? Kathy Gentile silver Martha Hill bronze while Greg Monino won a silver in the men's Your performance has inspired us all There's been a lot Now there's been a lot of talk too much talk in my opinion about the number of medals that weren't won the heart of the Olympics is The sport and the competition It's the exuberance of the hockey team it's Bonnie Warner breaking new ground in American women's luge It's the pride of Mary in Indiana at the performance of Wayne and Natalie seabold in pairs skating And yes, it's the spirit of Pam Fletcher who returned to the slopes two hours After breaking her leg in a practice run to cheer for her teammates It's striving with the support and love of family and community to be the best you possibly can be Because you love your sport and win lose or draw you love the competition and for that all of you get gold medals There's one thing you can remember above all Just being here in those uniforms and being where you were Put you a world apart From just literally millions of other people and every one of you has a right to be proud Thank you all being here and God bless you. I think winning medals is one thing, but I Think the thing that all and will most remember about Calgary is The outstanding support that not only all of our teammates gave us But that we got from our own country It filled me personally with so much patriotism and I think speaking on behalf of all the athletes that We're very appreciative to be a part of this country and we really respected a lot Bonnie Blair would like to make a presentation to make President Reagan a part of our team Mr. President as president of the United States Olympic Committee I'd like to thank you and I want to tell you the president of the United States that I think that we had 165 heroes representing the United States up in Calgary Men and women who won their regionals their nationals their trials and since then I've gone on to win world championships and and World Cups at each One of them. There's a hundred and sixty-five stories of personal struggles personal sacrifices not always sacrifice of their time but economic sacrifice and the Congress of the United States and you Mr. President have done something to really help the economic sacrifice And that's the passage of the coin act which is going to bring new resources to help support these athletes In their Olympic challenges and on behalf of the United States Olympic Committee and this team and the summer team going to Seoul I want to thank the Congress and the president for the coin act. Thank you very much


What an idyllic finish today Leanne. Fantastic here at Tickey Island. But before we talk about how we got here and what we did today I want to just revisit yesterday because that was awesome. Oh you're obviously talking about 90 mile beach. What a fantastic day we had. Awesome. So today we started at Mangal Nui school. And super to the show there with a bit of an interruption didn't he? Hey this is Garth Russell from Newcastle where I live and I know him well. I am the ABC Radio. In the middle of it kids loved it. I don't know whether he stopped the show I think he kept it rolling but he was including the ABC guy. I thought that was quite funny and it made it even funnier because we had no idea what the ABC guy was saying. Bring one side in. And we're raising a lot of money for second disabled children. So the next stop was Kai or primary school and the principal we gave away some bikes there. What else did we give away? Oh we gave the principal a good soap and we put him up the fish pot ladder and he got wet didn't he? I did just say to the guys it's the first time for many years that I wet my pants at school. So it was like work was over for the day wasn't it as we headed to Russell. This was a whole surprise coming to Tickey Island. A nice surprise though yeah. Oh great surprise.


Yeah, so I was gonna say it's kind of like a divide that's happening right. Yeah, you and Kenzo Yeah So, um, and Maya so knows like me Kenzo, you know the new girl wasn't in Miami and Wawa So, you know, yeah, yeah, oh and then it's like us for and then it's like Yeah, so what happened like how did it start splitting?  I don't know if y'all see you've seen that But yeah, we did that and we was cool like I don't know what happened But then somebody dissed me and ready to be comment under this song follow the bitch and then yeah I'm like what that's weird comments, too.        She's just trying to get some clown.  You don't be mentioning nobody dead. Yeah, I don't do that I don't do that.  Hey guys? It's Taylor. Make sure you follow us for more exclusives at WW dot talk of the town show calm like comment and subscribe


If I take a hundred of my patients that are having insomnia and I ask them what is the time you most often see on the clock they always say around 3 a.m. 255 301 303 so what is it with the 3 a.m. wake up that so many people are having within the field of traditional Chinese medicine there's a very specific cause that I thought I would share today. Hey guys I'm Dr. Alex Hein author of the health book master the day and doctor of acupuncture and traditional Chinese medicine so before we jump into this video there are two very important links below the video. The first is for a free guide I've put together for daily rituals that can potentially help you ideas to your life with traditional Chinese medicine and the second is if you'd like to learn about seeing me and my patient practice in Los Angeles or virtually via telemedicine you contact my private practice right below this video. Within the field of traditional Chinese medicine there's something called the organ clock. Now basically within the organ clock there is a two hour block dedicated to each organ function throughout the day so let's say stomach small intestine spleen kidney gallbladder etc but when it comes to 3 a.m. this is the transition from the liver time to the lung time. Now this may not seem to make any more sense thus far but within this field the liver is one of the organs that bears the brunt of a certain kind of stress and one of the ways we know that is that when our patients come in and we feel the radial artery for pulse diagnosis very often they will have either a tight pulse a wiry pulse or a fine wiry pulse and this tension that's manifesting in the pulse is a mirror of the tension in the nervous system the mirror of people living in a state of constant sympathetic dominance where stress hormones are running their life. Now I realize this is no surprise to many of us in 2023 that we feel stress more often and we feel tension more often and we feel more pressure more often but it shows up in the pulse as well and these tight pulses are often pulses of just nervous system tension in my opinion and chronic stress hormones. So we utilize acupuncture herbal formulas moxa physical therapies all kinds of interventions to help relieve the tension in this pulse but the tension is really a 3d manifestation into the pulse of this sort of pace of life right and this sort of quality that sometimes we're consciously aware of it because we feel it sometimes the body feels it the body's keeping the score but our mind is not aware that we are actually feeling that bad nonetheless this tension is sometimes called liver cheese stagnation so liver cheese very interesting you know back in the day typically it was women who are called hypochondriacs where complaining of a stuffy sensation in their chest especially in the ribs now this area of the hypochondrial below the rib side became synonymous with this idea of a woman being basically a hypochondriac making up these fake sort of illnesses but what's interesting is that within traditional Chinese medicine fullness in the ribs, distension, pressure in the ribs is a real sensation it is not it is not imagined is a real subjective sensation of diagnostic of liver cheese stagnation which is basically kind of like an emotional pressure and emotional stagnation a stress response in the body that can be physical it can be emotional it can be nervous system but we utilize formulas to treat this now the 3 a.m. time is one of the most common times people wake up when they have gallbladder, liver and triple warmer disorders within TCM gallbladder, liver most common issue that I see is upper GI reflux people are often waking up at 3 a.m. the other being the sanjiao the sanjiao the triple warmer complicated it's translate because it doesn't translate to a physical organ is more functional some people say it's a lymphatic system some people say it's the hollow spaces of the body whether it's the bowels you know some people get gas pains in the in the chest that's coming from the intestines that sort of thing but these three organs are most commonly the ones that we treat to relieve the 3 a.m. wake-up call now there are a number of ways you can treat this cheese stagnation if the cheese stagnation is due to stress in my opinion the best way to relieve this is not through meditation it's through physical exercise because physical exercise not only will help regulate the systemic stress response it increases blood flow increases the movement of lymph in your body and has so many other auxiliary benefits that will out perform meditation for the modern person and will result in people feeling more calm if it's not overdone now if this kind of cheese stagnation is due to digestion faulty digestion like I said upper GI reflux is one of the main causes I see of people who have this 3 a.m. wake-up they have a chronic upper GI issue if that is the case and this is not you know obviously environmental noise can wake people up but if their body wakes them up at 3 a.m. it is often an issue with the liver gallbladder and the triple warmer so the secondary cause if it's digestive is eating healthier meals not overeating especially later at night eating earlier in the night and for some people to blood sugar issue so eating a healthier meal that's protein and fat and is more Mediterranean in nature will benefit them as opposed to what they're currently eating so cheese stagnation due to stress more exercise cheese stagnation due to general GI upset is really staying away from alcohol eating earlier eating a smaller healthier meal so that is you know something that is eminently irrelevant I see lots of people waking up at 3 it's very easy to treat with compounded formulas from TCM and most often I just utilize my treatments as opposed to making them extensively changed our life so that is why the 3 a.m. it's not some weird demon or poltergeist it is there's something physiologically going on there and it's something that is eminently treatable with TCM so that's all I have for you today guys check out the links below the video and I'll see you soon