Hi, beautiful. Today, guess who's learning how to braid? You're doing it and I believe in you I know braiding can be a little bit stressful, but it's not gonna be like that today. I promise you, okay? Also today's video is sponsored by Netflix. Ya boi does Netflix deals It's like they have a new show called Cursed that is only streaming on Netflix I will tell you guys about that later on I'm gonna teach you how to do the regular three-strand braid then we're gonna move on to something That's a little bit more difficult, which is the French braid Honestly, it's not hard. I feel so confident that you're gonna be like Brad This was so easy like after this video once you learn the fundamentals and basics of braiding We're gonna be creating a look inspired by the courageous lead character of cursed Nimue and a little background about Nimue before we start She is a young woman with a mysterious gift who is destined to become the powerful lady of the lake And now let's get into the basics of braiding. Let's do it Hi guys, we have this Here to help me it doesn't feel right when she's done here. I love you. You will forever be my Okay, so we are doing the basic three-strand braid So to do this on yourself for the first time or like just learning how to do this Maybe you do purchase a mannequin. Honestly, you can get really cheap ones that are synthetic hair Um, I will link below for you. But also I would just separate your hair into two sections and braid it forward like that I like that. So two little pigtails on each side and that's how you can practice That's probably the easiest way to practice something like this So I sectioned your hair into a little, you know, half up moment just because it was easier for me to work with This much hair.  We're just gonna separate into three strands I've seen videos of people being like look separate into three strands like this like just separate it get on with the show Okay, you can do it. I believe in you. Cool. We have three strands now Awesome and I'll of course be using my BDSM bomb because this stuff will give you so much shine and a beautiful amount of flexible Holds to keep your style in place and it gets all these little flyaways down as you braid and it makes your braid Look really clean and perfect. I'll be using this just on my fingertips just to get the braid Going that way everything looks beautiful and perfect and shiny We got our three strands here. Make sure they're all nice and even do your best and now take a deep breath with me Let's do a little meditation real quick in through the nose Out through the mouth beautiful now. We're ready to braid every time you braid. I want you to do that for me I want you to breathe. I want you to get centered with your thoughts Okay, and have patience you may not become an amazing braider overnight It's just the reality of it all what we're gonna do obviously we have a center piece two side pieces and That is all we have all you have to know in order to braid is side over center repeat it with me side over Center what we're gonna do is put the side This side over the center. You see what I just did there We're gonna do the same thing again on the other side side over Center and you see how the center pieces change every time It's gonna be important that you keep your hand placement showing which ones are the side and which one is the center Do whatever feels comfortable to you This is how I hold it one hand holds two of the strands and the other holds one and you just put side Over center and that is how you braid you see that braid forming. Okay, cool The closer you keep your hands to the braid and see how my thumb is on the braid the tighter The braid will be side over center Side over center now your braid at first will not be as tight as mine This is definitely more of a pro version It is totally okay to have a really saggy braid the first time don't get frustrated Just work on holding the strands tighter and closer to the base of the braid So side over center side over center side over center side over You be a speed braider and you can speed braid how simple was that? I know you can do this and I know you're doing it right now and I'm so proud of you great job great work You are amazing Congratulations, you can braid now and if by chance you don't know how to braid yet and you're not doing it quite right rewind do it again And just keep practicing until you get it and then move on to the next step Which is the French braid again. These are the basics of braiding.  I've done that. I'm sure a lot of you guys came I'm sure a lot of you guys came to learn how to do a French braid I'm gonna take all this out I'm gonna section your hair into four quadrants and you should do the same to your hair And if you don't know what four quadrants looks like I will show you in a second how to do that You're gonna part down the center of your head Is that the center?  It's like is that a center? I don't know good enough for me. All right, we did that we did that good job If there's one thing you can do right today, I know for a fact it's the center part So actually maybe not even that so now you're gonna go from the apex of the head Which is the high point you can lay a comb on the head and see where the comb comes off of the head So right about here and bring it to behind the ear. Honestly, just make four Quadrants on the head. They do not even perfect like what I'm doing. Just get it done. Okay sounds good All right good, and we have our four quadrants. Voila. This is what they should look like now We will begin on one side of the head of your choice These are our practice sections and we're gonna keep it really simple at first We're just sectioning off a big piece at the top like this and making sure you have no knots in the hair Before you start or else you just want to have a panic attack while you're braiding and get so annoyed and then drop everything and just Cry make sure it's not naughty. We're gonna do the same thing We did before with the three-strand braid and following along with that side over center bit We did before and the only thing guys that we're changing about this is that we're adding a piece of hair on to each Side strand so we'll add a little something into our side over center song, which is pick up side center Okay, so what we're gonna do first is do side over center Side over center, and that's just two times to start. Okay. There you go. You got that a little tiny braid right there Now you do pick up Pick up and then that's added to the side Which then gets added what side over center Okay, so again that is pick up Add to the side and then side over center pick up Add to the side and side goes over the center. So pick up Add to the side side goes over the center and we continue and that is the pattern so pick up onto the side Side goes over center pick up side side goes over center pick up Side side goes over center and we'll just go all the way down because why not pick up side Side over center that wasn't the prettiest braid I've ever done But I was trying to explain what I was doing it. So don't come for me Okay, at this point your head should look something like this I find that this way going horizontally down your head is a lot easier to learn before you then do the French braid going back It is the same exact technique but getting your hand placement right before doing that French braid going back is a lot easier than starting off with that Kind of complicated like backwards on your head braid So I'm gonna quickly show you on the other side of the head how to do that exact braid one more time All right, we're starting off with our three-string braid. We're doing once side over center That is how you start the braid and then we are going to do what we're gonna do pick up side side over center pick up Then what do we do? We do side over Center and now we're towards the bottom of the head. So pick up side side over center pick up side side over Center pick up side Side over center. That's how you do it. You can do it So these are our two braids we just practice together and our little song went pick up side over center Pick up side over center pick up side over So keep practicing on each side of your head. You can undo your braids do them again undo them do them again it's just so much easier to practice on a horizontal like we did and You will continue to get better and better at this as time goes on Your braids may not look as tight as mine or as good That is totally fine yours can be all saggy and nasty or whatever But at least you're getting the basics of what we're trying to do and over time. You'll get better I promise you stick with it. You need to be the best braider in town, baby And I know that for a fact now. Let's move on to more difficult braids Even if you don't do this on your own head It's just fun to watch and you'll see how you can adapt these simple braids into so many different Variations and interesting looks so if you're ready to watch me transform miss Manny Quinn into miss Nimway think already Whoa, oh my god. Yeah, we colored her hair. She has a new brown color a new short haircut We are really trying to resemble Nimway's hair. She has this beautiful Dark hair at a moment on the top a nice warm brown into these blonde highlights towards her ends and her hair is really long So guess what we're gonna do? We're gonna add extensions into this look. We're gonna do it all up It's going to be very much Nimway's hair and I love Replicating character styles, especially with a look that was from so so long ago I just find it's so cool creating a natural looking style that could have been done without the modern tools We have today, so this is really exciting for me and even if you might not try this on your own hair It's just gonna be really fun to watch because we are going to completely transform miss Manny Quinn in to miss Nimway Okay in order to do this look We are going to start off with a center part now I'm not going to bring the center part too far back because that is not how Nimway has it We're going to attempt an exact replica of her look. Do I think it's maybe not going to be exact? Yeah, am I going to do my absolute best to make it look exact? Yeah, so we are going to just do a center part like that just about you know an inch back now that we have that done We're gonna then make two little triangles at the top of her head and this is what I mean by triangle It goes Doop doop see that it's a triangle Ha ha ha we are going to do the same thing on the other side and now we have you know one big triangle We're gonna now a clip of this out of place just like so we're gonna move on to the next step of nimway's iconic hair We are going to take our comb and section from the corner of that triangle All the way back in a straight line just like that and we're gonna comb this out of place And we're gonna do the same thing on the other side Bang bang and boom and now in the back We're just gonna section it out across and lift that up and that is our second section And that is like the hardest part of the whole thing in my opinion is getting the sectioning correct Um, but once you do that girl, we are smooth sailing baby. Wow So pretty let's get that middle part going again And now is the fun part We are going to take our hair and separate into three sections because guess what we're doing We're doing side over center. I'm gonna stick my hands into a little bit of btsm bomb I'm just gonna get them my fingertips that way. I can get all these little baby hairs out of the way And it will help aid me in doing her braids and making it all look perfect Because who doesn't love a perfect looking braid? We're gonna do our three sections and we're going to do Side over center side over center Side over center now. This is where it gets a little bit different and a little You know, maybe confusing for some people, but we're just lifting up hair from this side pick up side side Over center. This is what happens. We do not pick up any hair on this side.  Remember side over center pick up side over center Side over center and we're just gonna stop right behind her ear So as you can see here, I pulled the hair in this direction as I braided I went down and then went back up And that's going to give that kind of crown ethereal feel that Nimway also has and we are going to just continue this braid All the way back. We're going to discontinue picking up hair and now we're just doing a basic Three strand braid. All right. And now we have one side down of Nimway's braid She's looking like a strong powerful woman already and we love to see it We're going to do the other side now and we're going to do the same exact thing So I'll just speed it up and I'll show you just a little bit of it And that is the other side all Completed now what I've done here is just placed two elastics at the ends of her hair And I can just now let go of that section comb this back down This is all going to be left out And so now we get to tie these two pieces together But we're going to do something a little special today because I want my Nimway look alike to look exactly like the Nimway character So I did order some suede to wrap around her hair because back in those times that Nimway was alive They did not have elastic. So I want to stay very true to character Okay, and Nimway has the exact same kind of thing So from the front what we're going to do is not just pull them back. No, no, no, no, no We have to be extra with this We're actually just going to take the braid and twist it around once This will give the braid more of a 3d effect like sort of a crown effect like Nimway has it You see the difference here. This one's kind of popping out. This one's very flat So that's how you get that is just by twisting the braid around I'm going to do the same thing to the other side and I will bring them together in the back And I will put my elastic in this is looking so good. Yay I'm just going to double knot this and I'm gonna do a few little ties around just because I feel like that's what Nimway has in her hair. I was like analyzing as I was watching the show what she has done to her hair I mean her hair is so thick and beautiful Goals honestly and the thing I really loved about the show was I mean, I'm such a snob about like special effects and visual effects and whatever I love Good visual effects just like I'm sure a lot of you guys do and this show really has it They did not spare a dime on visual effects and the story It just comes across so beautifully because of how much work they put into their characters and into the set I mean, it's it's really good really dramatic really beautiful landscapes And that's what I really enjoy about shows like this She struggles with some of her identity But she really is trying to embrace it and it's just so beautiful to watch and see her character develop I just think she is such a strong woman and I just think her character is so cool and bad in that way Okay, I'm just gonna snip off the ends here boom and now she has this braid We're gonna take out our elastics And that is really the biggest part is doing that braid if you can get that down girl You are set you are set set set and that is nimwe's braid. Oh girl. You are looking fierce already But we're gonna make you look way fiercer. Okay. All right to finish nimwe's look off We're going to add hair extensions and I have the perfect nimwe color because she has those kinds of Light highlights at the end of her hair and we're gonna you know style it up do it up It's gonna be really fun. So let's section this off.  This stuff can be therapeutic You know, I love watching people finish off styles even if I'm not learning anything like I just love watching it So I hope you do too. I'm going to just braid up the underneath That way you doesn't show all of her short hair underneath and then I'll begin putting in the extensions We're giving miss nimwe inches honey inches and now I'll be using this tool by babe waves It is a very large crimper and we'll be giving her very soft crimped waves I have this on like 400 right now, but we're just gonna do a very light wave Okay, because nimwe has a very natural look.  So that is our goal, you know, she probably didn't have one of these back then I don't think so And we're just gonna kind of do like sporadic waves just like in random sections and not make anything look too perfect That looks so good to me. That's exactly how nimwe would have her hair. I'm just saying So good And for some natural, beautiful, tousled texture I'm gonna add in some of my salty sea salt spray for some separation This looks so good. Okay, let's keep going. Let's finish off the look Now she has some locks girl. Look at that hair. How exciting is that? I love hair Oh my god, that is some nimwe hair if I've ever seen it. I may be a little too good But you know, so we're gonna let down that piece at the top and I'm just gonna put a little pin in there So it stays down and I will show you guys in a second to finish All right, you guys, oh my god I kind of um feel like I slayed this like a little bit too hard almost This is the finished nimwe hair look this literally looks like the back of nimwe's head I'm kind of like geeking out right now Like you ever just get impressed with yourself when you try to do things I mean like look at these locks. Um, she has a couple hundred pounds of extensions in her hair and she looks so fierce This look is so damn cute. I am fully obsessed with it I love these crimp waves on her Oh my god and this long hair and the highlights and I mean honestly nimwe's kind of kind of doing a thing for me If she was on hair just for reacts, she would get a 10 out of 10 for that hair like look at her Oh Yes, honey Yes, give it to us and that is the top view you can see those braids go around like a crown and they meet in the back and oh my god Beauty you guys let me know how I did. Does this look like the character nimwe or what? I mean, did I nail it or did they not? I mean, I don't even know why I have to ask but just wondering your opinion Did I nail that as much as I think I did? All right. Thank you my love You look stunning now and get off of my set and I will see you in your show Streaming on netflix. All right, that is all for today guys and make sure you check out cursed streaming now only on Netflix linked below and let me know what you guys think about the first episode I can't wait to hear about it. I want to be able to talk about this show with somebody But nobody's seen it yet But now when I post this video you guys are all gonna be able to see it and watch it and tweet at me at bramblemyc So we can talk about the show and I want to hear your opinions What do you think about the first episode? Do you love it as much as I did? You can also follow me on tiktok and instagram at bradmondo nyc I also have a facebook page and a snapchat show under bradmondo So check them out and if you guys would like to purchase any of the x-mondo products in today's video like salty and bdsm Well, they are linked below for you and you can also shop at xmondo hair.com or go to our instagram at xmondo hair And that is all for today guys. Thank you so much for watching I hope you guys live your best extra braided life and I'll see you guys next time Bye This is club kid our dry shampoo. I like to mainly use dry shampoo for absorbing oils in the hair It really cleans the hair deodorizes it and actually softens it So it's just an amazing product to make your hair feel fresh and brand new again


Hey, Bolo buddies. Thanks for watching. All right, you guys I have a category Bolo video for you guys All right, let's get started It's an interesting one it's not one that you would expect it's probably not Something that you're gonna happen upon quite often But if you do you're gonna know to pick it up So we are gonna dig right in here and we are gonna get started on some Big money and bread and butter bolos, but some of these are such big money that you're gonna be like what is happening What am I seeing here? Okay, so my name is Courtney. My channel is based on items to Bolo for items to be on the lookout for items You can buy low and sell high So we are gonna get started here Okay, so the first item You guys these are nerds or no wait, I'm sorry. These are not nerds. These are double digits Cherry and orange kind of look like a nerd pack So I don't know if these are like generic nerds or what has anybody heard of these They're from 1986. They are unopened and They sold for two hundred and one dollars and fifty cents free shipping on auction. Hmm. Excuse me So if it were me I would put do not eat. Okay. Here we go It is it has never been opened the contents are old and should not be eaten I was gonna say I would definitely be disclosing that This is for advertising or just to look at nostalgia Not to eat. All right you guys so that one sold for 201 50 The next item are the nerds Glasses so these are sunglasses filled with nerds again from 1986 Check it out. These sold on auction for a hundred and thirty eight dollars and thirteen cents the next item is 1988 package of fleer razzles Candy gum and I believe these are all from the same eBay store so this person has probably like gotten really lucky and found some old candy at like an estate or somebody that collected it and they're selling it off and Luckily this person knew to sell these items because a lot of people just would have dismissed it and thrown in the trash Check it out. Does anybody remember these? Razzles The next item that sold are these vintage 1980s bag of 22 boxes of candy cigarettes. Do you guys Remember these I remember these from when I was a kid. They were actually really good But the whole idea of little kids smoking candy cigarettes is kind of a weird. It's kind of weird What do you guys think? Let me know in the comments. I Don't know I just used to eat the sticks. I thought they were good 105 50 on those 105 dollars and 50 cents you guys The next item is these Nick L Nip Wax bottle candy sealed. I remember these also You like There's like juice inside them, right? Am I thinking of the right thing? So crazy now these only sold for fifty one dollars, but you guys really fifty one dollars for some old vintage candy pretty good Okay, these are vintage 1988 Amur all am you are ol I have no idea box of annoyed candy balls Never heard of this one. Let me know in the comments if you guys have and tell me were they any good It says tart and fruity candy balls. These only went for twenty nine dollars, but still pretty good for some old candy Wow, that's sour candy by tops 1988 box of tops. Wow, that's sour sealed unopened again This one only had one bid and it sold for twenty four ninety five Now look at this one you guys tart and tinies by Willy Wonka 1990 and it seems like the Willy Wonka stuff does pretty well, but you can see here golden ticket sweepstakes So do you guys remember Willy Wonka in the chocolate factory? I know you guys had to have all watched that when you were a kid I wonder if that has something to do with making this more Collectible anyway, this sold for four hundred and eighty dollars with ninety bid you guys and this one just ended on January 9th The next item that sold are these bottle caps. I remember these also. I feel like they still make bottle caps today But I'm not a hundred percent sure these are by Willy Wonka also from 1988 again, I don't know if it's the Willy Wonka that's Is Willy Wonka candy still around is that's what's driving the price up? Does anybody know? This one still has 15 hours left on the auction and it's currently at three hundred and forty five dollars with ninety one bids so we're gonna go in and There are six unique bidders and what I like to do is I like to look at the bitter history and See how many feedback they have so this person has three thousand five hundred and ninety four feedback and this one has 454 so typically when people have a lot of feedback like that They're usually going to pay not always, but if this was zero feedback or ten or something lower I might question it but being that the feedback for the three hundred and forty dollars is over Three thousand I'm gonna say this is probably gonna be an item that gets paid for The next item are these rinky dinks again Willy Wonka 1986 and these sold for two hundred and seventy seven dollars So they are super flavored tiny jawbreaker jaw breakers Three fun foam balls. I don't know what that means candy with faces There you have it you guys two hundred and seventy seven dollars twenty three bids on that. Let's see The winning bitter had four thousand six hundred and ninety two feedback so Not too shabby there Another thing that you can do this just sold on January 3rd. So the feedback You may not find it yet, but we can go into their feedback and type in Well, we can look right here New raz. Oh, no, that's not it. Let's just type in Willy and see if anything comes up Nothing comes up yet. So they may it still may be in route to the buyer Okay, two packs of nerds you guys we got cherry and grape these are also Willy Wonka's and These only sold for forty eight dollars which sounds crazy to say that they only sold for forty eight dollars because I mean come on They're just some nerds, right? Alright, I wanted to make sure my screen share was up Okay, so forty eight dollars free shipping on that one again, I believe that is the same seller So they have just made a ton of money on old candy. The next item is this skeleton screw Sorry, excuse me Skeleton crew treats So the one thing about this I probably would have put vintage Halloween in the title just to hit the Halloween market But this is another type of candy. It only got seven bids and it sold for twenty six dollars It's funny because it's got the original 25 cent sticker on it The next item are these freckled eggs and these are also Willy Wonka's and they sold for thirty one dollars on auction And free shipping on those as well So pretty pretty cool and we're gonna go into some other types of candy here at the end of the video So stay tuned for that This is a cherry clan candy box. You guys. It's just an empty box from 1979 1981 So for forty nine dollars and ninety nine cents. So I'm not sure kind of crazy, right? The next item that sold are these red hots. They sold for fifty two dollars and seventy five cents with six bids these are from the 70s and They have the little valentine's boxes So they're just small mini rare The next item are these sweet tarts forty one years old 1980 vintage sealed bag of sweet tarts 70 mini packets halloween candy. This sold for 69 dollars and 95 cents Sweet tarts, I remember those The next item are these 1990 willa wonka willy wonka fruit flavored bumbles never heard of these candy bees Check them out. I mean who hangs on to this stuff for that long. It's just so odd, right? Um 98 dollars these went for in the buyer paid shipping The next item are the three musketeers. So these are candy bars um halloween box train witch So the halloween theme advertising on the box could have helped this sell for more this sold for a hundred and eight dollars with four bids And anytime you're selling vintage stuff, you do want to show the damage So the seller did a good job of showing the damage on the item But it looks like there's a 24 candy bars in here. So Or no, is this just the box? I think this is just this is just the box This is just the box Oh my goodness. No candy bars. Just the box guys 108 so Again, probably would have used vintage halloween as my first two words. I feel like people search that Okay, I remember these two chicklets Uh 1960s adams chicklets tiny size bubblegum pack still full vintage candy sold for 125 dollars you guys How fun is that? The next item is this clark candy bars vintage and this is just the box also So it's a candy bar box. So if you see these old boxes, they are collectible 146 dollars and 49 cents plus shipping on that one The next item are these 1970s certs. So these are candy mints you guys 12 rolls unopened Originally 10 cents. I don't know if that was 10 cents each or 10 cents for this entire box But this sold for 150 dollars plus shipping The next item are these rare hard to find vintage 1984 nerds willy wonka's candy treat size unopened box 189 dollars and 99 cents. So let's go over here to the feedback. Here's one way to check to see if something actually sold And you know, you can sometimes tell by the feedback of the buyer if you think it's going to pay But right here you guys three vintage 1984 nerds willy wonka packs sold for 250 dollars And we know that this was a good sale you guys because they left them feedback great item great seller So vintage willy wonka nerds really are a bolo And I don't see feedback for the one that we just looked at yet. So Not everybody leaves feedback. Um, so It's not full proof to decide if the item's been paid for but if the feedback is there You know that it was a good sale just one way to check. This is a runce Box it's a candy box and it says with t-shirt offer and This sold for 200 dollars plus shipping and it's from 1984. So I don't know I don't understand But I don't have to understand. I just need to know about it if I see it so I can buy it and I can sell it Here's another nerds you guys these are sweet and sour hearts So they kind of have the valentine theme. They are vintage from 1989 still sealed sold for $480 and let's go ahead and look at the bid history. The winning bidder had 454 feedback. So I'm guessing they probably paid Let's check the feedback here We'll put in Oh right here. Look at this Oh, nope. I'm gonna show you that one. I think yeah, I'm gonna show you that one here soon Let's type in nerds. Was it nerds? I think it was nerds Okay, so no feedback And no feedback for that. So no feedback but they only have 11 feedback in the last month The next item is this valentine's day conversation hearts store display. So it is just the display It looks like it has one box of candy left and this only went for $51. I would have thought that this would have went for more Oh, no, wait. I'm sorry. This is still live. This is still a live auction. It still has two days and 23 hours left So it still may get some action. There's 20 people watching this item So pretty cool vintage advertising piece The next item sold for $345. These are vintage valentine's day heart candy chocolate boxes from the 1940s to the 1980s A lot of times people will sell these individually depending on how fancy they are They can go for more But this person decided to lot them up and a lot of 20, which is pretty cool $345 is not too shabby. I'm not sure why the shipping's so high but um I don't know Maybe it's an oversized box Vintage Christmas lifesaver sweet story book all rolls in cellophane Tanger Pretty cool This is you know, it's a collectible because it's Christmas. It's vintage, but it also still has the original Lifesavers in it and this sold for 102 dollars and 50 cents plus shipping All right. Next I want to talk to you about farm girl Scavenger Noelle's channel. Noelle is a bolo finder you guys if you're not following her you need to go over and check out her youtube channel because number one she knows so much about so many things, but she also sells like things that Maybe you wouldn't pick up or expect to sell. She's just very knowledgeable But you guys know how I do category videos like this one She does this but in a different type of style. She goes live and she picks a category. So, um This was a state sale bathroom So these were items that she picked up at an estate sale and she unboxes them and shows you guys different bolo items to look for from The bathroom and I have some bathroom Category videos also so check those out but also go over and check out hers because we talk about different items but um I will try to remember to link her down below if I don't remember Please somebody remind me in the comments or just search farm girl scavenger. Noelle and you will find her So Noelle sold these tic-tacs and if I remember correctly she was at like a Place where she picked up a bunch of these wholesale and I think they were a quarter of piece I was supposed to write that down and I forgot but I think that's what I read Noelle correct me if I'm wrong down in the comments But this is tic-tac bubblegum you guys and I'm pretty sure she said these are discontinued flavors And she sold it for 119 dollars and 99 cents plus shipping. All right, let's look at the next one She sold two of these for 109 dollars and 99 cents are these tic-tac sugar-free gum 56 pieces and She has sold two of these so far two packs for 29 dollars and 99 cents plus shipping The next item is this wrigley's hubba-bubba max strawberry watermelon bubblegum and it is Wubba bubba wubba Or no hubba. I said wubba hubba bubba gum and you get five packs for 22 dollars and 99 cents and uh She sold two of these and the buyer paid shipping The next item that sold is this tic-tac gum. It's spearmint again. It's discontinued. This is a sugar-free gum And this sold for 29 99 for two packs and she has sold five packs of these you guys Or five separate listings The next item are these tic-tac mixers cherry cola gum again I do believe these are a discontinued flavor and she has sold two of these for 25 dollars and 99 cents Yeah, pretty interesting, right? People selling candy and gum for big money All right, you guys What do you think? Let me know in the comments. Have you ever sold candy for um big money or I don't know. I have another video. I think it's called vintage gum And you guys at that time I found all of these Vintage gum listings. You got to go check out that video I'll try to remember to link it down below if I forget somebody remind me and I'll try to grab it and put it In the comments, but thank you guys so much for being here. Be sure to check out some of my other bolo videos That's basically what I do here on this channel is I Look for bolo items. I sell on ebay full time So I have a bunch of what sold videos if you want to see my bread and butter videos Those are items that I sell for 35 dollars or less Or I've got my big money bolos, which are those higher dollar items that everybody hopes to find super super cheap So check out some of those. I've got featured members videos where I feature bolos of my members And let me tell you they find some big money bolos. They are awesome So check those out check out my channel If you're new here, be sure to subscribe like comment and share and thanks for watching Be sure to check out the description of the video like comment share and subscribe. Thanks for watching


What's up everybody, JCM for JaspysCasebridge.com. We're just doing the last filler here guys, only filler we had to do to sell the last seven spots in the Big Head Express with this 2023 legacy football, one box break, number 19.  There are two hits, I believe, two autos and 12 cards of pack or 12 cards of box, eight cards of pack. But like I said, at the end, what everybody's waiting for is that we'll randomize the 32 names and top seven gets to the Big Head Express. So let's roll the dice and let's do the break first, four and a five, nine times, one, two, three, four, five, six, seven, eight, nine. Aaron, done to Aaron, nine times, one, two, three, four, five, six, seven, eight, nine, nine, 49 is out of the Seahawks. Stick to what you got, alphabetize it up quickly, let's just see your teams, then I'll quickly do the break. All right, just sold two spots, Danny and Stacy got spots in the Breaker's Delights. So again, Breaker's Delight now is down to two left, straight up, and we just gotta do the filler, which is down to 17. I think definitely doing that Breaker's Delight today, guys. The box of legacy and again, like half of the checklist does have all the team logos, the other half usually just all collegiate, which again, we're gonna go to the teams that are currently on Tuesday Clifton Longus, but for the most part, the RPAs do have the logos, that's just kind of more like the base and number cards in some models as well. Dare to tear, Dalvin Cook. So tear if you dare. Out of the 65 Minnesota Vikings. So Minnesota Vikings, that's gonna go to Alamedsuit. Got a little Generations, Raiders, Randy Moss and Duonte Habs. To 25 under the lights, with Anthony Richardson. Got a blast in the past, Bob Lilly for the Cowboys, number to 249. Ray Guy auto, great iron grates. Also, I think they were stuck together. Jan Hampton for the Bears. So Bears, that's Allen, Raiders, that's Chad Coleman. I think he's gonna get a bonus auto. Those are stuck back to back. So it's kind of cool. There you go. Warren Moon, Seattle Seahawks edition, to 100 Little Mini, Matthew Stafford, to 125. Raiders, eight in a column. Futures patch autograph number to 100. All right, we got Geno Smith at 299. And Josh Downs, to 35. Obviously Jimmy G has a history of injuries. And let me look at that Josh Downs really quick. Indianapolis Colts, a little extra auto there. Very solid, guys. Now, all right, so again, top seven, get in, roll, six on the four, 10 times. One, two, three, four, five, six, seven, eight, nine, 10. 10 times. Josh Smith, Seattle's Richard V. But Nicole, you're in. Allen Douglas, Chad B, Allen Brandon, and Chad Coleman. That was the randomizer there. Coming up next is the Big Head Express number 11.


Gun smoke brought to you by L and M filters. This is it. L and M is best, stands out from all the rest. A round-dog city and in the territory on west, there's just one way to handle the killers and the spoilers and that's with a U.S. Marshal and the smell of gun smoke. William Conrad, the France-Grogg story of the violence that moved west with young America and the story of a man who moved with him. I'm that man, Matt Dillon, United States Marshal. The first man they look for and the last they want to meet. It's a chancey job and it makes a man watchful and a little lonely. It was a 90-mile ride from Dodge to where Chester and I were taken cover behind a small knoll just beyond the Smoky Hill River. In front of us, hidden behind a horse, he'd ridden to death covering those miles was a stranger called James Nation.  It was a rough ride and we'd caught up with him the hard way. My horse was dead from a rifle bullet and Chester was standing on three legs crippled with a slug in the shoulder. Something Mr. Dillon, he's in as bad a spot as we are. Now watch him Chester, he's moving. Now wait a minute, don't shoot. He's got his hands up, he's giving up mighty easy. Oh maybe. Let's go meet him. Better not try nothing. He'll die if he does. Come on Nation. My hand's down. Put him down. I come here to shoot it out. I don't like killing. He killed a man in Dodge. Self-defense Marshal. Not according to the witnesses. It was all friends of his, they tell you that. I heard him say it. A man looks guilty when he runs Nation. You don't believe me. It's not up to me. You'll get a trial. I'm still alive but a long way from Dodge. My god that's right Mr. Dillon. How are we going to get back anyway? Now we haven't crossed the Overland Express route yet. We'll walk on till we find it and then wait for a stage. We can get to Fort Donner, we can borrow some horses from the army. Maybe the stage won't pick us up. Now that sure won't pick us up here. Marshal. What? You don't owe me no favors Marshal. I'd like to ask something of you. Yeah. I've never been arrested before.  Kind of ashamed of it. That's not my worry. I know. It's only a little thing I'm asking. Won't make no difference to you. Would you, when we get on that stage and all, would you not say nothing about your taking me in? Don't let him know I'm a prisoner. You've got a lot of guts to ask for any kind of a favor. I'd be powerful, grateful to you Marshal. It doesn't matter any. But you make one bad move and you'll go to Dodge in a sack. I won't give you no trouble. I swear I won't. You'll get a fair trial. Well if it is a fair trial, I'm going back to California. I don't know why I ever left. Justin.  He stood there long enough. I'll go with Nation get his guns. All right. I'll gun you anyway Nation. That was a mighty good horse you shot. I'm sorry. All right. Come on. Let's get going. They got a long way ahead. Miracle tip. Much more flavor. Ellen M best. Ellen M is best. Stands out from all the rest. Ellen M's got everything. Everything. Everything. Best flavor. Ellen M stands out for flavor. The miracle tip draws easy. Let you enjoy all the taste. Best filter. Ellen M stands out for effective filtration. No filter compares with Ellen M's pure white miracle tip for quality or effectiveness. Best tobaccos. Highest quality tobaccos. Low nicotine tobaccos. L and M tobaccos. Light and mild. Every way. Ellen M is best. Stands out from all the rest. How easy they draw.  Ellen M is sweeping the country. It's America's best filter tip cigarette. That stage is headed right for us. We have to keep walking. I never heard of a stage driver who'd leave the road 10 feet for anybody. I guess you're right, Marshal. You sure must be mighty proud of that coach. Look how he's gone and painted it bright red. What if he won't stop for us? Road agents don't carry their saddles. The driver will notice that. If he's awake. Driving six horses ought to keep him awake. Yeah, but not sober. Where'd they come? Not bandits. Where's your horse? Remember, Marshal, what you said. Oh, they lost him. I think the one in the middle ain't even armed. Pick them up. Okay, Wells. It's your treasure box. 60 miles to Donner. You're crowding us some. So that'll be 10 cents a mile. Six dollars, huh? Six dollars a piece. Second advantage of a man doesn't seem to bother you very much, does it? 17, 17, 18. For a couple more in here. A shot to be made to walk. There's room for two if we jam up, but we sure can't handle three. No. You can see that for yourself, Mr. Yeah, you're right. Chester. Oh, no.  I have to climb. I don't want nation on top. So you got up there. Yes, sir. Come on. They aren't charging less if I got a ride on top like a dog on carpet bag. Company guarantees a ride, Mr. Comfort. Your problem. Get on it.  The bell is up long enough. All right. Get in, nation. Son, just we now probably fall over here and bust open on your side and say, but it's dulling. Oh, I'm simmer. This old goat here calls himself fly. My name is fly.  Well, at least he's sober.  They only wake up long enough to pull on that jug of poison they got.  Uh, what business are you in, Dylan? Questions, questions, questions. Zimmer, you got the manager of a pool. Nobody's talking to you, old man. And I don't have to listen to your problem.   You can't scare anybody who's lived as long as I have. 85, Dylan. Would you believe it? No, sir. I sure wouldn't. I knowed Mary Wether Lewis, Dylan. Met him in St. Louis when he and that Clark fella came back from the West Ocean. West Ocean? Listen to him. Why don't you leave him these, Zimmer? Oh, don't pay him no mind, Dylan. He's been nervous about something the whole trip. What are you talking about? You got ants all over you, Zimmer. I can see him from here.  Ain't gonna talk to you no more. And Dylan? Yeah. We'll be at Cherokee Station in about an hour. Spend the night there. Grub's terrible old.  One pilgrim claimed to store belly.  Didn't even know what the old woman there in the cookin' told him. Real polite like she said, well, then you just help yourself to the mustard, my friend. Cherokee Station was a long, low mud hut with a flat roof, thatched and linsotted. The one room served as an eating place in the daytime and a bunkhouse at night. It was hot and stuffy inside.  I hate to think of spending the night in that place. Well, if I look a drowned in that air, I'll bet that stuck dinner ain't washed since the day he was baptized. Ain't it awful? Don't worry about it. We'll sleep outside. Good. But half the night you're gonna stand guard over Nation, Chester. Well, I ain't gonna run away, Marshal. You run once. All right. I'll tell you something. Then maybe you'll trust me a little. What? Want to know who Zimmer is? I mean, who he really is? John. Name's Art Carp. How do you know? I've seen it under his picture. It was tacked on a pole out in California. I think the reward was five hundred dollars, but I ain't certain about that. But you're sure it was his picture? Of course I'm sure. Everybody was talking about him for a time out there. He's real clever, that carp. Now, how's he clever? Well, I heard that one time he rode in a stage as a passenger, and then some fellas on horses, they stopped the stage in a canyon somewhere. And this carp pulled a gun on the people inside the stage and held them there. And when the boys outside got the treasure box unloaded, he got out, climbed on a horse they had for him.  And that's pretty clever, ain't it? Yeah. Yeah, that's real clever. Seems to me you went through a lot of trouble. They could have helped it up without it. Passengers sometimes give bandits more fight than the driver and messenger, Chester. They feel safe because they're inside the stage.  I think you're telling the truth, Nation. But I'm not going to arrest him. Not yet. Oh, there's that poor, miserable old woman. Oh, yeah. But she can do something for me. You keep an eye on Nation, Chester. I'll be back. Yes. Evening, ma'am. Evening. I wanted to ask you to do a favor for me, ma'am. Yep. The messenger Wells, he's inside there drinking some of your husband's whiskey. I'd like to talk to him alone and without the others knowing it. You want me to fetch him out? Well, I'd be grateful to you. Yes, ma'am. I'll do it for you. Thank you. Okay. What's the trouble, mister? Come outside, will you? Okay. Say something about whiskey for me, Hank. This'll do. Tell me something, Wells. How long you been riding shotgun? Two years? Not that it's any business of yours. What'd you do before that? Oh, I mean, that's the last question I answered. And I'm just trying to find out if you can handle yourself. You keep nosing around. You'll find out. Look, Wells, I think you're a good man and I sure hope I'm right. Who in Thunder are you, anyway? You know, my name is Dullin, Matt Dullin. I'm a U.S. Marshal, Wells. A U.S. Marshal? Prove it. Now, I'm traveling light. I got nothing on me to prove it. You talk right out, don't you? All right. What do you want? How much are you carrying in your treasure box? Say, you really do move in, don't you? Are you either trust me or you don't? $50,000, Marshal. Good. Now, what's the best place for a hold-up between here and Fort Downer? What? It'll be Willow Crick, I guess. What is this? You think we're going to be held up? I don't know, but tomorrow you make the driver go through Willow Crick like he was on fire. Chester will be on the roof to help you and I'll sort of organize the passengers inside. How do you know all this, Marshal? Well, I'll tell you when we get to Fort Downer. And, by the way, don't say anything about this or me to anybody. All right. I guess you're the boy's sister, Marshal. Thanks, Wells. Good night. Good night, Marshal. I sure hope I ain't made a mistake about you. Oh, Wells. Yeah?  I've heard of that stuff freezing solid on a cold night. Ellen M. I've got Ellen M. I've got Ellen M. And Ellen M. got everything. Best filter. Notice the color of the miracle tip. It's white, pure white to give you the purest and best filter. Best flavor.  And that's what every smoker wants. Best tobaccos. Highest quality tobaccos. Low nicotine tobaccos. L and M tobaccos. Light and mild. Today, by Ellen M. It's sweeping the country because it's America's best filter tip cigarette. Yes, today, why don't you get Ellen M. Because Ellen M's got everything. This is it. Ellen M. filters. Ellen M's got everything. It's the best. Next morning, Wells told me it was 10 miles to Willa Creek and another 12 miles to the next change of horses at a small swing station run by a lone stock tender. We went through Willa Creek flying. Had no trouble at all. But trouble was waiting for us in the swing station. It was a small one room adobe hut with a usual corral at the rear. But as we pulled up, I noticed there wasn't a horse in sight and out of the two windows facing us was pouring thin streams of white smoke. Everybody jumped down and ran up to the half-open door where two arrows were embedded in the frame. It's Comanches. Look at them arrows. Stand back there. Let me take a look inside. Let's all take a look, Simon. Look at that. Three men killed. This man's still alive. That's the stock tender, Dylan. Wait a minute. I thought he was alone here, Wells. He's always alone here. I'm wondering who these other two men are. Never mind them. They're dead, ain't they? Yeah. They're dead. Maybe he can tell us what happened, Dylan. The stage is here. As Indians, I never even heard them. Who were the other men? The white men. I'll even be, Dylan. The man's dying.   Who were they, fella? What were they doing here? Road agents. Tell me up. They're gonna hide here and hold up the stock. Pardon the stage. What else? Did they say anything else? Put my head down. Turn him loose, Carp. There are too many of us here. Take it easy, Dylan. This fool's nervous.  He means it, Dylan. All right. What are you gonna do now, Carp? How'd you know my name? I know my name. I know my name. How'd you know my name? I know more than your name. Oh, don't matter. I want you men to walk out that door one by one. As you pass by me, I'm gonna take your guns. Get started now. What do you think, Dylan? We better do it, Wells. All right, men. Let's do what he says. That's better. You first, Dylan. Sure. Get outside with Dylan. Marshall?  What? Pretend to join him. Do you trust me that much? I have to. All right. You men line up there. Fly, stand in front of me. I want to tell you something. Say it fast. You know why I wasn't armed? No. Because of him. Dylan, I'm under arrest. What? Dylan's a Marshall. Bringing me in for killing a man in Dodge. I've got a better chance together than you have a lot of you. I'm warning you, National. You do that, and I'll run you down if it takes a year. You won't even know where to look, Marshall. You will be standing around here till tomorrow while we're riding off on them stage horses. How about it, Carp? That's your name? I heard the Marshall say it. That's my name. Okay, Nation, grab a gun there and get over here. Sure will. Now don't you try nothing, fly. I'll shoot you as quick as Carp would. Carp.  I want your gun, Carp. Crazy? No. He ain't crazy. Don't turn around, Carp.  What is this? Drop that gun. Go on, drop it. He means it, Carp. Shoot him and I'll put a slug in your spine, Carp. Might be worth it. Make up your mind. You're awful willing to die, Marshall. What about you? How do you think I'd die? No.  I'm never gonna drop that gun, Marshall.  I'm not a murderer, Carp.  You did fine, Nation. I'll remember it. Now give me the gun. You still gonna take me in? Sure. Well, that don't seem fair. You know I have to. Why could a road off with Carp? I'll be at your trial, Nation. I promise you. I'll tell him what happened here. I'm standing here with a gun. You ain't even armed. No, it's like you told Carp, Nation. You're not that kind. Here's a gun, Marshall.  Do you? Well, then I've got an idea how you might get over that. What? Go over there and pick up another gun. What for? You're gonna be the first prisoner I ever trusted to wear a gun, Nation. And besides, you'll need it to guard Carp till we get to Fort Donner. William Conrad. You know, Father's Day is June 19th. And if the old man's anything like me, he'd sure welcome a carton or two of L&M's. They're mild and plenty quick on the draw. And L&M's pure white miracle tip, well, no filter stacks up with it for quality or effectiveness. Believe me, L&M's got everything. Flavor, taste, mildness. The best possible filter. Give them to Dad, Father's Day. Put a smile in your smoking. Next time you buy cigarettes, stop. Remember, only Chesterfield is made the modern way, with Accu-Ray. This amazing quality detective electronically checks and controls the making of your Chesterfield, giving a uniformity and smoking quality never possible before. For the first time, perfect smoke column from end to end. From the first puff to the last puff, Chesterfield smokes smoother. Chesterfield smokes cooler. Chesterfield is best for you. Next time you buy cigarettes, stop. Remember, Chesterfield is made the modern way, with Accu-Ray. Put a smile in your smoking. Just give them a try. Light up a Chesterfield. They satisfy. You'll also enjoy Chesterfield's great radio shows. 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We review a selection of methods for performing enhanced sampling in molecular dynamic simulations. We consider methods based on collective variable biasing and on tempering, and offer both historical and contemporary perspectives. In collective variable biasing, we first discuss methods stemming from thermodynamic integration that use mean-force biasing, including the adaptive biasing force algorithm and temperature acceleration. We then turn to methods that use bias potentials, including umbrella sampling and metodynamics. We next consider parallel tempering and replica exchange methods. We conclude with a brief presentation of some combination methods. This article was authored by Cameron Abrams and Giovanni Bussi.


I personally was in a dangerous situation. Obviously I have reported from war zones in places. I went to Iraq, I went to Afghanistan, things like that. It's not like the reporting that I did, I was ever threatened with personal danger. I did have a couple of instances where sources of mine were put on trial or were charged with criminal acts for providing me with information that happened here in the United States. I had to testify in those trials. In one case I testified on behalf of my source. In the second case, the case never went to trial. It was ultimately dropped, but my source was unable to work for five years or so. It's not dangerous, but it was very disturbing. It was a case of the U.S. government at the time trying to thwart the flow of free information. It sent a chilling effect. That's the closest I could say come to danger.


Bismillah ar-Rahman ar-Raheem In the name of Allah, the All Merciful, the Ever Merciful And they have a lasting curse beyond the Satan and his followers Allah's blessings and peace be upon Muhammad the Prophet And upon his pure family Our respected viewers, before getting started with this episode Allow me to start with one of the sayings of the Master of Martyrs Imam al-Hussain, when he says Allah the Almighty has initiated the concept of commanding the good And forbidding the evil as a compulsory duty for us As he knows that if these two duties are well performed And established in all other divine duties Whether difficult or easy, shall be well established as well And this commandment and forbidden shall invite people to Islam And secure the rights of the wronged people As well as our opposition against tyrants and transgressors Our respected viewers, peace be upon you And welcome to Imam Hussain A series of episodes in which we talk about the different stages Of Imam Hussain's life Starting from his miraculous birthday Then talking about some of the stories and events Happened to him during his life And then mentioning some of the prophetic quotes Said in his right Until we end up with his unmatchable martyrdom My name is Hassan Hadi and I'm honored to be hosting this program Our respected viewers, in the last episode We spoke about why Imam Hussain had to escape Medina Heading towards Mecca Due to the political pressures the Imam was put under And we also spoke about Imam Hussain's Paying pilgrimage to his grandfather The Prophet of Islam However in today's episode we will continue talking about The same topic yet more elaborately Our respected viewers, after paying pilgrimage To his grandfather's grave Imam Hussain next visited the grave of his beloved mother The mistress of the woman of all the worlds Fatima al-Zahra al-Batool alaihi salam He stood before her, her gaze Reflecting upon her oppression Tears streaming down his blessed face Imam Hussain alaihi salam had witnessed her oppression Namely his mother At the hands of the tyrants, hypocrites And disbelievers of Medina He had washed, namely Imam Hussain As her inheritance was stolen Her husband's right Was usurped Her house was attacked Her body crushed And her unborn child murdered Imam Hussain alaihi salam had washed As she went out with her husband Ali ibn Abi Talib house To house trying to gather support From a nation that had turned its back Upon his prophet And the chosen family of Allah the Almighty As Fatima al-Zahra alaihi salam Had suffered For what normal human being could bear To see his mother go through such oppression While her station was even more exalted Than that and her suffering even greater And even in death She had none that awaited her blessed son Hussain alaihi salam And the horrors and his family Would face later on in their life Our respected viewers Thus the Persian Muhammad al-Mujahidi Composed the following verses poetry About the Imam alaihi salam As he stood at the grave of his mother He says the following It was a horrible dark night With dead silence surrounding it Al-Hussain ibn Ali was there With tears flowing down from his eyes His mother's sad sound of wailing Filled up the whole place It came loudly from her grave And wandered in all directions Finally our respected viewers Imam al-Hussain alaihi salam Visited the sacred grave of his brother Imam al-Hussain alaihi salam To pay his final respects Before returning home There he composed his final will And testament to his brother Muhammad ibn al-Halafiya In it he wrote Namely Imam al-Hussain My up-spring against Yazid In it Imam al-Hussain alaihi salam Wrote the following The uprising against the Yazid Is neither for the sake of Creating fitna and corruption in the land Nor for amusement Or to satisfy my own ego Rather it is for the sake Of reforming the affairs Of my grandfather's nation After handing the will to his brother He left Madinah With his family filled with sorrow As well as a firm resolve As he left He recited the verse From the Holy Quran Then he left it out of fear While he kept on the look out He said my lord Saved me from the unjust people Peace be upon you O master of martyrs When you were born And when you were martyred And when you will be resurrected Our respected viewers This is the end of today's episode Let's pray that Allah the Almighty Will bring the experience Of the master of our time To interpret the message of his grandfather When he says I only desire to spread good values And to prevent evil Thank you very much indeed For joining us Wa salamu alaikum warahmatullahi wabarakatuh


It's okay. Hello everyone and welcome to this week's Spitesize Talk. I'm very happy to do today with us is Jonathan from Sekera and he is going to talk about the NFCore pipeline differential abundance and off to you. Thank you for the introduction. Yep, so I'm going to just spend a quick few minutes talking about differential abundance which is maintained by myself and by Oscar Wacker. So before I kick off I just wanted to clarify some terms. By abundance here we're talking about the the magnitude of matrix values. I'm aware that abundance is a bit overloaded in some communities but here it's just a term we wanted to use to be more generic than expression. When I talk about features I'm talking about the individual variables and measurements of the other matrix which is in the expression space I'm talking about genes or transcripts and by observation we're talking about the individual samples or experimental units and by covariates we're talking about a variable that possibly predicts the outcome under study but it's not of the primary interest. So for example batch is one that comes up quite often in expression data and just kind of reinforce that if you're used to dealing with expression data in the R world you might use this sort of structure where you have a central assays concept where the matrices are stored you have features which is where the metadata associated with genes and transcripts are stored and you have samples which is where the the observations are stored and in kind of the core world that's a sort of a sample sheet basically these observations are metadata there. Another important concept for differential analysis is the concept of contrasts so in the top part of this slide we have a sample sheet I've highlighted the condition column here and in order to to define a contrast we might use that condition column and say that we want to compare the samples that have control in that column and the samples that have treatment in that column and to extend this very slightly we have this concept of blocking in the sample in the contrast variables we use here which is used to incorporate the sort of batch covariates into the differential expression modeling and we'll get to that a bit more than more later. So the objective of this workflow was really to act as a unified point of access for differential analysis of matrices with diverse data types of which are I think is is is the most widely used but we we also have expression arrays and there's a proteomics pathway through there as well but basically we want to share common components in these sorts of analyses across data types so when we have matrix filtering we have graphical representations like volcano plots and these can all be shared across different different data types and then we have some some high quality reporting at the end that we want to use to make all this all this pretty. So and so yes we do have this kind of similar approach across data types you have a process that gets data you have some exploratory analysis going on you have to filter the matrices in some way often removing zero all zero rows and we want to compare compare groups and produce a report a report at the end and that's common across all across all these data types. So the current status of the workflow looks something something like this if I could get you to concentrate just on this on this middle row here we have four key inputs to the workflow we have feature annotations we have the abundance values we have the observation annotations so the sample sheet I just mentioned and we have the contrast definitions and these all four get combined we we validate them to make sure these things are consistent among each other we filter the matrices to to do this sort of zero filtering and so on that I mentioned and then there's a multiple different or currently two different differential analysis methods used depending on the input data type and then we proceed to optional gene set enrichments and exploratory analysis differential analysis and the the final kind of reporting steps. I also want to highlight the this top left corner of the tube map here this is just the different input methods that we have that can be used to generate these these these primary inputs basically so for example we have an input method using the get you a module which uses a geo identifier to pull a matrix from from here and we have a pathway by which you can specify aphor matrix array intensities and they again get converted into a matrix which can be used in the downstream analysis so these are just kind of different entry points into the into the workflow to generate these common inputs the generic usage looks like much like a lot of other and of core workflows we have an input sample sheet here we have a matrix which is the dependence values and the gtf file so the the gene annotations and then the contrast definitions that I mentioned before obviously we have a lot of other parameters but these are kind of the key the key inputs that you that you have and then this will will work in a minimal sense um to dwell a little bit more on the r and a seek case um r is the end of corny seek module outputs um it's it's matrices from using the tx import our package or tx i meta input uh package um and that is useful for accounting for the biases in length of transcripts across samples so it's very important to adjust for cases where the differential um isoform usage across treatment groups can lead to differences difference in length so if treatment group a um uses primarily very short isoform and treatment group b uses primarily very long isoform that can introduce statistical biases in terms of counts which we need to adjust for um and the best way of doing that right now is to take the raw counts to come out of of um the entity workflow this um salmon merge gene council txv and also pass into the differential abundance workflow the transcript length matrix and that allows that modeling to occur um we only added that fairly recently into the only seek workflow so as a second best for older versions of only seek you can just use the the length scaled counts that come out of that workflow um there is now a um a proteomics pathway through the through the uh through the workflow uh built by uh by oscar um and that allows you to take data produced by max font um use the protease package to convert that table into the abundance matrix and normalize that matrix and then pass that matrix into all the downstream um differential analysis and reporting that I've I've just quickly mentioned um before any matrix gets to go further through the workflow we have to do this disvalidation so just to emphasize that a bit more we have to check that the feature um annotations that we provided are compatible with the rows of the matrix that the um sample annotations are compatible with the columns of the matrix and that the um the variables that we've used in defining our contrast are actually present in the sample sheet for example so all that thing all that happens before before anything else goes goes through the workflow um then kind of the first real sort of analysis step is to do some do some filtering um the default is just to remove all rows with all zeros um but there are more more slightly more advanced options available so you can specify a different threshold rather than zero you can specify a number of samples that must pass that threshold or you can specify a proportion of samples that must pass that threshold and that's often quite useful in RNA-seq data where you want to if you have a hundred samples for example and and you want to to say you know that your smallest treatment group size is is is 10 you can say that um you want at least 10 samples to pass the threshold because that would be a quick amount amount to one of your treatment groups passing with passing threshold you could do things along those lines um for differential analysis we have d-seq which is currently used for RNA-seq and other undefined input types and we have lemma which is used for the affimetrix array data and and the proteomics data but both these modules have a consistent interface they accept contrast in the same manner um and they model covariates in the same manner and we might anticipate that as other data modalities get added to the workflow in the future that we had other specialist differential modules um just a note on batch handling um it's bad form that went in the sort of analysis to do an actual batch correction prior to differential analysis instead we model batches of covariate as part of the differential analysis using models that look a bit like this in in dc current or in lemma um but it would be nice to have an actual batch correction in place to to help with exploratory analysis so for example when you're doing pca to remove the effects of that batch in in doing that pc we don't currently have that but we but we do plan on on doing that some point in the future um downstream of the differential stuff we have g-centered enrichment analysis um we've had the g-sea tool in here for a while that's just a wrapper around the brode the brode's g-sea tool um and that's not based on thresholding of of genes it's based on um um sorry that's not based on thresholds it's just based on ranked ranked gene lists um in the data so that that's that's that's uh good way to go um but also uh osca has also incorporated this g profile to method into the pipeline recently it's in an unreleased it's unreleased so it's not in a release version of the workflow right now um but that is based on thresholds so it takes um um gene sets and compares them against the background um one of the reporting outputs of the workflow is is a html report derived from an r markdown file um important that we importantly we also provide the um the r markdown file itself with all the parameters resolved um at the end of the workflow and that allows someone to take that markdown file and the results files that are bundled with it and and customize it to do their own type of analysis or to tweak the plots or someone which can be quite a quite a powerful thing to be able to to do um because we also we can't anticipate all the things that people might want to do in their reporting um another thing option that we have is the shiny ngs package i built that in about 2016 many years ago um and that is a an r package which builds shiny applications based on standard inputs so you give it a set of matrices and some p-value tables and um gene sets and analysis if you have them in the right format um and that will automatically build um an own uh shiny data mining tool um and that allows you to to modify thresholds used to select gene lists and and and produce plots from subsets of the of the genes and and so on which is which is quite nice and the workflow if you if you configure it correctly it will actually push an application directly to to um shinyapps.io which is their kind of platform as a service offering by the RStudio people that will actually so you can have the workflow produce those things automatically the result analysis which is which is really um can be quite nice i should say that i haven't shiny ngs is is an independent product and it's quite powerful i haven't wired every feature of it into the workflow yet so the the things you get from the NFCore differential abundance workflow not all of that connects into shiny ngs yet but but it is um to a large extent there and you can do some quite powerful data mining with it um and you get you get plots like this so this is a volcano plot um uh which if if i was showing you it live uh you'd be able to mouse over these points and find out what genes these are and you can adjust these thresholds to um to move these boundaries around in the plot for example to to show different sets of sets of genes and you can change the color palettes and point sizes and do all that that cool stuff as well and that's powered by plot plotly um and you can export those plots um from this point of interface so to do um we do need to optimize the workflow for larger sample numbers it's currently doesn't the current doesn't perform very well once you get above tens of samples um we need to fix that so that there's adaptive reporting so we don't try and um use some of the plots we have right now for very large sample numbers um we need to cut down the report size somehow um i think probably some of the html can be reduced by rounding some some some of the values i'd like to add some sparse matrix handling uh we have some more gene set methods that people have asked us to add um i want to modify the way that we use gca or at least i have an option to do so so that we use the ranks derived from parametric fold changes from dc and so on um and i want to improve the the shiny ngs in integration of gene sets which is not currently currently wired in um i would like to add the batch correction as i as i mentioned um and uh so the engine of cig is also working on an alternative pathway through this workflow using log ratio analysis um and hopefully that'll be available sometime later this year uh just to give credits i built the initial workflow structure and some of the differential modules osca has since done a lot of work on adding a number of improvements especially in the reporting stages and that proteomics stuff i mentioned as adine was a former colleague of mine at helix and he built the digio query functionality and also it's always necessary to thank the also municipal community members for all their bug fixes pr reviews and and for implementing all the cool standards um i should acknowledge my former employer helix who who funded me during the first phases of development of this workflow um and that was also as an employee he was as he was doing that work uh i'm currently employed by sequera who are a great company with great providing great community support and we should credit osca's employer cubic at the university of tubingen um okay that's me at the end thank you very much thank you so much that's really interesting i love the shiny up really um so uh everyone from the audience is now able to unmute themselves and uh to ask any questions maybe i start with the one that is already in the chat so it is asking uh if sva is used for batch correction for example so that's one i think you could do yes that's um yes so to add that batch correction for use in exploratory analysis yes that could be sva it could be combat any of the sort of commodity batch correction tools yeah thank you um i also have a question so how many ways do these comparisons go is it just like a paired comparison like with two sets or can you have more than two sets you can have any a number of so it's only pair-wise comparisons but you can have as many pair-wise comparisons as you want um you know so as long as you put them in the contrasts file you can you can compare in a number of different ways people have asked for time series analysis that's not currently possible in the workflow um so with the kind of um likelihood ratio tests and so on and we haven't come currently implemented anything like that okay thank you are there any more questions from the audience uh victor is asking uh if he if it doesn't support dose responses could you could you maybe expand a bit on that victor oh well if you have a dose response asked to the school testing for instance so if you have 10 different doses seem like a continuous career effectively exactly yeah yeah no it's just it's just discrete contrast right now yeah thank you um are there any more questions from the audience


Everybody forced here rip in 2022 select the baseball hobby six box number 13 random team style There are the results of the random and you can always find them at late in sports cards comm Thanks everybody for joining good luck Sold out for the night so the next group breaks that are available. Everybody is on Tuesday night Tuesday night no breaks tomorrow The shop is closed tomorrow Silver diamond level rookie twins Joe Ryan Chas McCormick rookie jersey card Houston and Fuego silver for the Giants Brandon Crawford tri-color rookie white socks Gavin sheets light blue rookie D-backs Drew Ellis to 1 49 Rave Spencer Strider rookie Jersey Otto out of 99 nice Strider He is certainly in rookie of the year contention Zebra Concourse Red Sox rookie Connor Siebold Ego Red Sox Silver premier level Freddy Peralta Brewers Concourse Red Brewers Josh Hader to 1 99 Philly's to 99 rookie Jersey Otto Matt Veerling Frontmill Reyes Silver Concourse D-backs Mad Bum Premier level white Braves Ronald Acuna junior to 50 nice Acuna Tri-color concourse for Cleveland frontmill Reyes Jazz Chisholm and Fuego Seattle rookie Jersey card Matt Brash to 250 and Mullins insert Rangers rookie Jersey to 250 Glenn Otto Silver select stars insert Astros Alex Bregman Tri-color for the Braves Eddie Matthews. That's Milwaukee Braves Red concourse for the Marlon Sandi Alcantara to 1 99 Rookie Jersey Otto for the Nats Riley Adams to 1 99 Got Atlanta Jersey Otto Tucker Davidson to 2 99 Shane Bieber Concourse Silver for the White Sox Louise Robert Concourse Silver Angel Shohei Otani Light blue Nathan Ovalde Red Sox to 1 49 premier level Tri-color premier level Corbin Burns Brewers O'Neal Cruz Jersey card for the Pirates rookie not numbered Premier level Silver Mets Jeff McNeil And a white premier level Nats rookie Josiah Gray to 1 to 50. Sorry excuse me to 50 Cleveland Eli Morgan rookie Jersey to 250 silver numbers insert Freddy Freeman for the Braves Tri-color Sonny Gray Reds Um Zebra is usually the case hit parallel. We had lime green Chris Bassett A's to 99 It's usually a zebra parallel. I don't know if it's actually a case hit, but it's about one a case Nice lightning strikes red Sox Xander Bogart short print inserts Nice one red Sox exited the game earlier today. I hope he's all right. Silver premier level d-back Zach Gallin What's up sin Victor? So we pulled a zebra Connor Siebold earlier. So I think that that would count as the case hit Blue jays Jose Barrios to 99 I was watching this Invicta break last night Dude, he was pulling so many twin s-scrooges Diamond level silver Trevor Rogers Miami Silver concourse rookie for the Reds tj Fridl Red premier level for the Jays Tiosca Hernandez to 199 Yeah, but for two boxes, that's pretty good for a blaster and a an omega Uh, Tri-color rookie Seth beer for the d-backs Hold one of those golden you twos last night too Did he really? Marcus Strowman cracked ice patch to 15 Cubs Marcus Strowman Game worn on that one So I imagine that's a Mets patch Redemption going out to the rays wonder franco rookie Jersey Otto. Hello.  Ah, yeah I imagine most of the time with the with the megas. It's mem then Usually it's probably like what 75% of the time you get mem 25% of the time you get an auto or something and that's not even Accounting for points so To 250 jersey card angels rice alley glaciers Moonshot silver giants chris Bryant Tri-color to ask her. Hernandez blue jays Red premier level jordamond gummary yankees to 199. Oh, see that's insane The know-how will hobby and no huddle. Yeah It's a 99 conor c-bold rookie jersey auto red socks And flago Kyle Lewis. I think you might have to stop ripping prisms in victim. Maybe stick with like blasters or something Silver concourse mad bump for the d-backs Yeah, getting a member of the blasters not a killer or the getting the mem and the mega not a killer because it's you know, it's mega Uh lime green kiki Hernandez to 199 red socks Yeah, silver diamond level miami trevor rogers I think next paycheck. I'm gonna buy a few, uh, pokemon booster boxes and rip them Tri-color jonathan india reds Looking at some like brilliant stars and actual radiance Haven't written much of that stuff Juan yipez the 250 rookie jersey cardinals Yeah, go is tough too, man Silver premier level sabrador Perez royals It reminds me of them every single like not like main set but like subset So far and sword and shield has been tough It's high died. Uh d-backs zack gallant to 20 It was like a champion. It was a champion's path. I think is what it was called It's a 99 blue jays patch auto alehundro kirk nice hit blue jays Again should be starting the american league All-star game as the catcher there Or the all-star game with the american league catch not the american league all-star game something Champions path and there was another one. There was um, I can picture the box in my mind. I just can't think of the name Yeah, those are tough, man silver concourse john means orials Lime green jaren durand rookie for the red sox out of 99 premiere level duran's been doing well lately Should be the leadoff hitter going forward for the red sox. I would imagine try color red sunny gray O'Neill cruising fuego jonathan india reds base auto to 299 Yep, that's what uh champions path is pretty sure josh low rookie jersey rays Caleb would know but I don't have time to go chat with Caleb right now He's he's on the other side of the wall right here, but I can't I don't have time brand of belt lime green the 99 giants White sox jersey auto for Gavin sheets the rookie to 299 There's like a scratch From the rookie card logo down to his shoulder. I'm gonna send an empty box and wrapper with that one for the white sox Maybe panini will replace that one for you white scratch, but still a very noticeable scratch The silver premiere level blue jays Nate Pearson back to ice Yankees Aaron judged at 25 nice judge Oh, okay. Yeah. Yeah, you're right demon. You probably know more about than I do. Anyway, uh, silver d-bags delton varshow concourse Yeah, so I was thinking of that other one. That's what I was thinking of I was thinking of the non gx. You're right. Reese can air rookie jersey padres the 250 silver numbers Nolan aronato cardinals Tri-color a's frankie montos Dude, I've seen a bunch of uh What is it darkness of blaze at like my the closest publics to me recently? It's kind of weird The one on maguire road is that a bunch of like to just lose darkness of blaze. I'm like, it's kind of strange You can find pokemon cards about any place people sell anything nowadays. I feel like sports cards kind of but like Pokemon is much easier to get I feel like with the blister packs and stuff Find them all over I mean, they've always carried them a little bit to some extent of like wall greens and cbs and all that but I've seen them at like circle k's and stuff Yeah, dude, they have them like everywhere silver concourse ryan sandberg cubs. There was one like I went to this I was stopped at this gas station one time kind of in the middle of like not a whole lot diamond silver level josh bell nationals And uh, yeah, they have like I think they had etvs in there something They're like tins or something. That was weird tricolor sedric mullins orials So carlo stanton Exactly demon you can find it everywhere concourse white frankie montos to 50 a's You just can't find like sealed boosters and stuff everywhere Oh, we have back-to-back autos here. I think we do Connor seabold red sox silver sparks To 149 and a sparks read debt mers to 299 to 199 for the angels Look at that two autos in the pack Kyle lewis and fuego I would imagine that means we get an extra hit right if they're back-to-back stuff like that probably an error to kupita marcano cracked ice patch auto to 25 pirates With the padres patch on the pirates card. We've got a hazy sanchez to 149 marlins red concourse brewers tyrone taylor to 199 Yeah, it's definitely more common now for sure. It's not as pricey as it used to be some of those sets Logan Webb jersey card to 199 giants and fuego silver for the braves ozzy albies So i'm gonna buy some ripsome Shry color mike trout angels ashore adence brilliant stars. I might take a crack at um got vedal bruhon rookie jersey rays at uh What is the other the one before those two? silver premier level hezoo sanchez marlins Uh fusion strike that's a fusion strike. I might try fusion strike again. I don't know Some of the hits are really cool now, but I had some tough luck with fusion strike and um evolving skies evolving skies Destroyed me dude. I got nothing good out of evolving skies. They're like I reflect three boosters some etbs I would buy blister packs here and there whenever i'm with the target The best thing I got was like a secret rear garbodor dude. It was so bad. It was like bad bad Like it would never happen again kind of bad It was it was brutal dude brutal Anyway, enough about that uh relics vedal bruhon logan web Uh riz canair josh low vanya pez ricelli glacia smarka stroman elie morgan oniel cruz glenn auto matt brash and chas mccormick All of the autos now we had to kupita marcano Redetmers connor siebold gabin sheets jonathan india alehundro kirk connor siebold joziah gray tucker davidson Riley adams matt veerling spencer strider And a wander franco rookie jersey auto hollow redemption going out to the rays big congrats to tampa bay That's gonna do it another slide coming up


My name is Jeff Hemingway. I'm the sole quality specialist here in South Dakota and today we're actually going to look at our our sole quality and water movement model Demonstration here is is is really set up for small groups classrooms Farm shows that kind of display and we'll just take it apart and actually look at the model and see what the components actually are Okay, as we're doing this the part of the model with hydrologic connectivity We're actually going to fill each one of these jars up with different soil components I've actually got a conventionally tilled soil a no-till soil. I've got soil. That's very high in clay and The basically a sand and depending on what you actually want to demonstrate You can use these components to actually look at those individually or in layers such that we can actually look at At water movement to look at confining layers, etc So basically what we're going to do right now is we're going to fill these jars up with Those components and then we're going to go back to looking at water movement through those those individual samples So as we fill these these jars up you need to keep in mind that We want the water to move through the soil and not preferentially around the outside So make sure that as you fill these these things up We do have the samples in there firmly and don't end up with voids around the outside of the jars So that water moment is through the soil matrix as we fill those up Okay, now that we actually have each one of these jars filled up With some different soil components. Let's talk about what we're actually trying to demonstrate here First of all is as we start looking across there There's all kinds of combinations you can actually do but these are just a few that you can actually do and we'll just talk about that the first one here is we just went through and we put that coarse sand in and we'll actually Look at water movement fairly rapidly through that that that sand and we'll talk some more about that But really that the whole issue there is that we have large pores. We really don't have any any aggregates we're talking about it's it's Very clean sands, so we're gonna actually have water move through there fairly rapidly The next one we actually have is we have a no-till soil And that's what this one is and it's it's has really good aggregate stability It's a loam something that we'd be looking at from an agricultural production standpoint is a very good soil And really what we're trying to demonstrate there is some really good aggregate stability and water movement through that anyway The next one here is conventionally tilled and then the comparison is going to be just this is that that as we start looking at This conventionally tilled soil water is not going to move through it as readily because it doesn't have that aggregate stability And you're going to actually see some differences between water movement and ponding of water itself So that's really what we're talking about there The next two demonstrations here that I actually have it's that we're really looking at not only water movement But looking at that water movement through and and talking about confining layers and different layering associated with soils This one is is our conventionally tilled soil again right here same thing water is moving through that But then we're going to hit a clay layer and we'll we'll look at that again a little bit here But you can actually see that that difference of Color in this particular case because that that that high That soil high and clay actually has this more gray color you can see that layered in there water is going to move down Through that it's basically going to stop the same thing is actually going to happen if we have a sand also We start talking about porosity and moving from a a fairly fine poor space to a large poor space Water needs to move from a gravitational water standpoint Because it's held by tension and when you move into that large poor space It's actually going to go from field capacity to gravitational water and then it'll become super saturated Before it actually moves into that next layer that is so radically different as far as poor space So we can demonstrate that with this one also We could use a straw layer in there In other words buried residue would do the same kind of thing So let's start looking at these things as we start adding water Okay, so if we actually look at the sand itself, it's very easy to see water movement down through this It's a good demonstration. It's really rapid movement because of the the coarse Sand material actually then see it start to move through that soil profile fairly rapid fairly apparent Water movement, we'll just keep adding water here and you'll see it move right straight through that profile and of course as we have water move down through that it'll come fairly soon and we can actually start to see it come right out of the bottom water moving all the way down through fairly rapid really coarse Then we'll let that drain out Let's go on to the the next sample we actually have it and this is a no-till sample That has really good aggregate stability in other words the higher amounts for gaining matter when we're looking at something That's hydrophobic to water. We're gonna end up with water moving down through that soil really fairly rapidly good permeability Frankly high permeability associated with those soils now from a conventionally tilled standpoint because we've oxidized or mineralized that are gaining matter You're gonna actually see poor aggregate stability and water movement associated with that is also going to be slower So we can actually demonstrate that by looking at both of these samples together, and we'll just start adding water and See what happens? Okay, as I look at my no-till sample. I've got water movement down through that that profile and again It's coming out the bottom fairly readily Let's look at our conventionally tilled sample here Actually, we're ponding water on the soil surface because we don't have that that soil structure and a few drops have come through but We really don't have any water movement Because it's sealed off that aggregate stability has broken down and we're ponding water In other words our infiltration rates are basically gone to zero where we can continue to still infiltrate water Through that no-till soil that has that really good aggregate stability and you can continue to see that actually occur over time In the last two samples that we actually have are really looking at differences in Soil textures we know that we can actually end up because of differences in parent material We can end up with differences in layering in other words. We can have Either soils that are radically different in texture From one horizon the next and like say in this particular case. We actually have layered a Layer of soil that actually is really high in clay In our conventionally tilled sample. This is our conventionally tilled sample, but here we have a layer of clay What's going to happen with this sample is that because of that clay has a very fine texture It has a high shrink swell capacity. It's actually going to Act as a confining layer. It's going to stop water movement down through that soil profile The same thing is actually going to happen with our next sample too Our other sample that we actually have set up here is has our conventionally tilled soil over top of a sand And what happens and again in this this the sand that we actually have as water moves down through this this Profile that we've set up we go from a fairly fine Texture that is a loam And then we move to something that's really core something that has really large pore spaces and because of those differences We're going to actually limit water movement and in fact when we start talking about that It's it's that water movement goes through that soil profile and because it's it's it's Moving gravitational water down through that profile It has to get to a condition of being super saturated before it's actually going to move into that larger pore space And we can demonstrate that With that sample so it's kind of a cool thing. We can do that with Residues also So let's look at these samples So again here, we're doing this on our clay layer and again you can see that Through our funnel system. We're moving water into the soil profile in a my left here doing the same thing With our sand Now at some point you're actually seeing preferential flow But you can also see around here on the on the sides here You can actually see where that clay layer actually is we've stopped water movement down through the soil profile And that's actually part of what's occurring Over here. We had some of the similar with what's going on with our sand again, we're moving Moisture water down through that profile. We're hitting that sand layer And right now we're perching water. You can actually see that is actually we've got pond and water on the sole surface Just keep adding water on our our clay layered sample over here should end up being doing the same kind of thing It's starting to pond water. You're fairly rapidly and like I say the cool thing is what we're looking at here Is you're actually looking at you can see the the wetting front that we actually have moves down through And because of the coarse texture We're actually have a water being ponded on the surface Kind of a neat demonstration This is actually the third component of this model on hydrologic connectivity and permeability other two components were capillary activity and aggregate stability Within this model Functionally, we're actually looking at at some really good demonstrations and Whether using this in a classroom or at a farm show. I think this model will actually be a very productive And actually a really good demonstration When we're start talking about soils soil differences And conditions that you can actually manage and change


Rachel Colissi, 7 Facts About The Woman Who Captured Sia's Heart Sia Colissi has had the spotlight on him ever since his rise to captaining the spring box. After the World Cup win, the attention has become global. The attention is also turning to the force behind Colissi, the strong woman who has been his rock for many years, his wife, Rachel. While Rachel has been in the media for being a no-nonsense wife, she is also a sweet mother and supportive partner. Briefly.co.za gathered a few interesting facts about the First Lady of Rugby. 1. Childhood. Rachel Colissi was born Rachel Smith in Graham's Town on June 2, 1990. She has two sisters and one brother. 2. Early life. 3. After completing high school, her family moved from Graham's Town to Cape Town where she studied events management. She worked as a marketing executive before resigning. 3. Religious affiliation. Rachel identifies as a Christian. She works with various charities and donates what she can, whenever she can. 4. How she met Colissi She met Sia through a mutual friend in Stellenbosch. She said that she always knew he was destined for something big, and her prophecy came to be. 5. Colissi marriage and children. She married Colissi in 2016 and resigned from her job to become a full-time mother to her children, Nicholas and Kezia. The couple took Sia's younger siblings Lyoma and Lafilo into their home after their mother passed on. 6. Controversies. Mrs. Colissi is no stranger to controversy and has seen herself fighting trolls online over her husband. She is known to be hot-headed and quick to stop women from flirting with her famous hubby. She has also had to fight racists who have had issues with their interracial marriage. 7. Social butterfly. Despite the controversies online, Rachel enjoys using social media. She particularly likes Instagram, where she shares adorable pictures of her family.