أجل والدين will be starting in 10 minutes this Lush Doha thing So ladies and gentlemen, start-ups Hopefully we will have everybody in the front row ورش ساعت Welcome to the start-up scene and today is the slush day Right? So we will be starting our first activity for the Innovation Theatre 2014 And I would like to welcome our start-ups for competing today To be in the biggest start-ups and investment events taking place in Helsinki in 2014, November 2014 So I don't see Abdulaziz. Where is Abdulaziz Shalhoub? Yeah, Abdulaziz. Welcome aboard So today we have a start-up competition This competition originally was designed to be between regional start-ups So we had invited three Saudi start-ups and three Qatari start-ups Unfortunately, two of the Saudi start-ups were not able to make it for various purposes That came at the last minute So we will be having four start-ups instead of six And I mean, we will be having a lot of activities We will be having a talk presented by Intel Capital باريش اكسوي The Senior Director for Investment in Intel Capital will be there with us very soon And we will be having a very interesting panel discussion on how can you fly your start-up globally So I would love to invite Henry, Henry Holm Representing the start-up sauna foundation He will be orienting us on what is Slush And what is the aim of Slush Doha And then he will take us on this interesting journey Henry, thank you so much Good afternoon everyone Good afternoon My name is Henry Holm I am here to present the start-up sauna foundation Finland I would like to spend a few minutes with you just to run through the agenda for the afternoon So we will be covering a little of the background of the start-up sauna and the Slush event That gives a little bit of the glimpse and insight to the cold winter event now in a late spring early summer here in Doha Also, we will be going through various stages of the evaluation of the ideas and the start-ups We will have a chance to coach and discuss Every time when you present your own ideas and your ideas of your company there's a possibility for new discoveries Some of the old ideas becomes obsolete and some of the old ideas becomes actually the new ideas which are then the prominent and the drive of the company and the product what you present At 4pm we will have the pitching competition It's a 5 minute very intense straight to the point pitch Why would I invest to this particular event or company or product? And like mentioned earlier we will have a panel discussion about going global and then finally announcing the winners for the event Without any further due I also want to send regards from the CMO of the start-up sauna Martin Talvary Martin was here today very early morning hours but because of the visa regulations he was not able to enter the country with the 72 hour visa which we thought would be possible so my apologies for that but the presentation covers all the key messages what Martin wanted to leave with us So, Slash and Start-up Sauna Program So, Start-up Sauna is an organization which was created around one country Finland in a very crucial state I would put it this way because the country was taking a lot of youth into a company called Nokia and that saga which lasted from mid 90s till 2007 8.9 until now being a part of the Microsoft Corporation a handset division took a lot of talent from the schools and at the time when we went to the schools and we asked students how many of you would like to be an entrepreneur very few raised their hands and at the time you know the entrepreneurship was not seen as a very glamorous choice it was seen as a different choice to the one where people would take large corporates but every time when we look back into the nation's growth it is important to recognize that everything starts from small and we need to incubate the ideas and the spirit of entrepreneurship no matter what so we remind ourselves also in the Start-up Sauna our meeting room one of the meeting room is actually looking like a sauna it is a place where you go you sit down you share your ideas and thoughts the whiteboard a good wifi connection and you may book the venue at your wish and this is part of the whole idea how this is put into a conversation it is a conversation where we want the ideas to find the ones who want to invest and then also nurture the ideas because not necessarily is the first idea the best one we run twice a year program we start the spring program after the March and April local events in May now it's finishing in May June 4th starting on May 5th and during that 5 week intense program not only we learn a lot about the new Start-up Companies but also the Start-up Companies they learn a lot about themselves so the founding members get to know each other in a totally different depth and even ideology because sometimes the ideas have to be driven by a much deeper vision or passion than just making money and during this July there's a Silicon Valley Visits and then we start the second program in October and the first program actually finishes into a slash event in November these 70 plus local events accumulates about 10 Start-ups per event 700 Start-ups coached we get about 1,800 applications that will actually be twice as much going forward now and those 8 patches which we have done so far over 100 alumni companies has graduated and if you look at the investment amount it's still a relatively small amount but we talk about smaller economies investing into the companies so in a prorated ratio actually it is already a significant amount we have 100 investors and venture capitalists who are helping to start-up companies to grow and then coach them and this is really to share the network what these people have built or each one of us have built over the years on doing the international business and here's the catch we are a non-profit and it is an organization which is not taking equity in your company so each one of us participate to this one to bring your company into a greater new heights and giving you the opportunity to actually grow then about the slash slash event is something that during the cold winter month of November there is no reason really to go outside it's cold, it's miserable it's slashy, it's icy weather so it's a good idea to stay inside and innovate make your best ideas to flourish and come and share those with us in 2008 and 2010 it was very very small almost in the event where people who were really enthusiastic about building this new ecosystem of start-ups and putting this vision of 2020 we will have a flourishing very new breed of businesses coming out of the new countries it was just a matter of tens of companies coming together 2011 and 12 it became hundreds so suddenly we realized that 3,500 visitors 550 companies has been with us and the numbers are growing now 2013 we had 7,000 attendees which we expect now to be 15,000 this year 68 countries crossing 100 hopefully during the next event and then 1,221 start-ups crossing then 2,500 this major event gives an opportunity for then matchmaking 1,221 conversation between the investors and then the start-ups again we take no stake on that we facilitate, we give the premises Finnish government supports the and the Helsinki government supports the event and gives us the venue overall 70 billion dollars of worth of assets these venture capital firms are presented in the event also large media coverage so why to do this we believe that we have to build a really well-functioning start-up ecosystem across Eurasia and what do we mean by well-functioning is actually connecting this ecosystem then to the rest of the world developing ecosystem there is not just one ecosystem there is actually many and us uniting our thoughts and ideas as well together we can actually generate more wealth more also opportunities and more jobs for the future generations so all this voluntary organization hundreds of people they put their own personal time and thought to make this all happen and we would like to then of course to share this thought with all the competitors so from today's event the winning company will be joining us to the slash event in November 1819 in Helsinki and let's see what it's all about I think we have the energy so who would like to be in slash in November I would love to guys I would love to invite our competing start-ups to be there in stage so I will start with the guest I will start with عد العزيز from Saudi I will invite سعدة وسلحب so I would love to welcome you all and wish you a very interesting slash journey and please present your start-ups to the audience and bear in mind that there is audience voting so I think we should show that audience are contributing into this so عد العزيز please proceed do we have a mic please first of all I want to thank you for inviting me from Saudi Arabia to this great and fantastic event my name is عبد العزيز أرشل هوب I'm from Saudi Arabia I graduated from King Saudi University from computer science and today I'm going to present a new idea that I just started around maybe 4 months from now it's called عد سوق the idea is about connecting the advertiser and publisher through the social media and maybe we'll continue more about the idea when we present it at 4pm إن شاء الله and again thank you I would like to thank the organizers of this event for giving us the opportunity to present our companies today and I am the co-founder and the creative director of Garnass Company we are a creative local Qatari studio that makes local games and creates local content and we have launched a few games already in the market من يرز من فرنسي من يرز دهسري the managing director and the co-founder of Garnass you know what's the challenge today the challenge is that usually in Qatari when you have you're hosting someone usually let them win but today unfortunately we cannot let you win I'm sorry this is the first wifi advertisement company in Qatari we both met in stars of science the scientific show which is assured by Qatari foundation for young scientists and entrepreneurs to develop their ideas and we decided to take one of our ideas a bit further and actually implement it in the market so we've started we're keen to present it to you and looking forward to to present it in maybe a few minutes السلام عليكم مجددا as my partner said actually briefly we're here to win we're here to go to Finland I'll see you there wait for us okay السلام عليكم I'm Akhil I'm co-founder I'm a startup with an idea called ticket fun which is like the most happening thing here because it's events what we do and we do online tickering with recommendation engine like the first time in the Middle East and the region so watch us out for the presentation and we're looking forward for Slush yes السلام عليكم my name is Hamid Rwadi founder and CEO of ticket fun and we're here to compete with these gentlemen and we're here to win and we will win thank you very much great so we'll be having four winners then I like the energy so I'll pitch for Ablaziz so we'll be partners together we invited the three Saudis we would love to have them on board thank you so much thank you so much guys


Well, hello and welcome to understand men now. I'm Jonathan Astley of Jonathan Astley dot com and I'm so excited to be doing this live Stream for you today our topic the six deep questions to ask a guy to build attraction You got to start with these six. Well, you don't actually have to start with these six But these are six good ones. So all right really quickly if you're new to my YouTube channel Please hit the subscribe button hit the bell so you can be notified of new content And if during the course of this video, I share something that resonates with you Please hit that like button Okay, very quickly for those that are joining me live or those listening to the recording I do the content bit first then I take questions. So Play right now, I'm going to cover the content first and then we're going to go to questions. Okay, so let's talk about What kind of questions you should ask a guy?  They really do a terrible job. And how do I know this? Because tonight's friday night There's probably going to be hundreds of thousands of first dates throughout the world or at least here in the united states and Significant number of them will never get to a second date And even a significant number of those second dates don't make it to a third date And even a significant number of those third dates don't turn into a lasting relationship And one of the reasons is is most human beings these days date on a very surface level very surface level I mean and let's face it the dating apps You know kind of perpetuate this You know like when when you swipe someone and they've swiped you and whether a woman reaches out first or the guy reaches out first And their best first line is high Like really that's the best thing you can say now I know if you're walking up to a total stranger At a nightclub the first thing you might say to someone is hi or you might say hi My name is jonathan or something like that But here in the dating world, you know when you've got a dating app or a dating profile There's a lot of data listed there. There are things you can draw upon to start a conversation And yet most people do a terrible job at that now. I'm not saying all but most okay, but as we progress further I've noticed that one of the things that women do poorly at Is asking really good questions because you've been many of you've been told that asking questions might sound like an Interrogation or an interview. Let me refrain that most people say well if you ask questions, that's an interview Well, I want to get real here Dating is an interview process. That's what dating is the purpose of dating Is not to have a good time the purpose of dating is decide if you want to explore a relationship with someone And the purpose of a relationship is to decide if you want to explore a long-term commitment with someone These are the purposes of it. So if you want to really build a deeper richer foundation of trust connection and attraction Then let's go beyond the surface of hey, how's your day? Oh, how's your day? I hope you have a beautiful day I really do hope you have a great weekend. Hey, have a great weekend. Hey, are you doing anything fun this weekend? Like really like that's the best you know And I want to share with you all something, you know, like I have a meme I save on my phone here I'm going to pull it up real quick. Where are my favorites? Uh Bear with me Where do favorites show up? Oh, here we go. So this is one of my favorite memes.  I don't want to know what's up Okay, so while I share this with you is I want to encourage a deeper approach to the dating process instead of things like this You like sushi.  Oh my god. I do it every weekend You like the rolling stones? I've seen them in concert five times. Wow. We must be so compatible with one another You know, this is just simply what I I call as familiarity or areas of where we have similarities with another Have you have you ever had that where you went on a date where you felt like you were so similar? But all it was is you just had these familiar things But you really didn't have the deeper connection of shared values blendable lifestyles and emotional maturity If that's ever happened you please hit that like button so I can know right now and By the way, when you do hit the like this gets shared Oh really quickly. Um since my audio had messed up last time. I'm trying a microphone right now Let me know if the audio is okay by reporting it in the chat box. Okay, so let's jump into those six deep questions You want to ask a guy? And then I've got something to share right after that. So let me put on my trusty glasses and number one What's the one thing you're most passionate about? What's the one thing you're most passionate about by the way ladies This is whether it's a woman asking a man or a man asking a woman. I love this question because Let's face it having passion in our life having purpose having something that that drives us Or at least brings us joy is something worth celebrating So when someone says what's what's something? What's one thing that you're very passionate about? I know for me, it would most likely be amongst other things would be my son I would share that and why that's so important is giving a person an opportunity to share what's Passionate for them in their life gives you a sense of what brings them joy What brings them joy? This is so much better than the surface questions Like what kind of car you drive or what do you do for a living? Not that that's not a bad question to ask what you do for a living, but I want us to go deeper And in sales they teach their several techniques of called mirror and matching mirror and matching And also what's called drilling down drilling down And why I say this about sales is one of the things you have to become in the dating process is a good sales person And what I mean to say is a You're selling yourself to some degree But also a good sales person knows how to qualify a prospect They know how to qualify a prospect and what I mean to say is assess if this person is worth investing in Does this resonate with you? Please let me know hit that like button So what I love about the passionate question is that it allows you to go deeper than the surface that most people do And these six questions are going to be really fantastic questions to ask Okay. Now I want to say this some men Revel in the questions because they're narcissists So they love that you're asking them questions, but these men are very single focused They'll answer your question from a place of vanity But they won't come back and ask you the follow-up questions that go with it So that's one type of person Okay, then there's the weak, you know type of man who's afraid of his own shadow, which you're not going to want to Date him anyway He might you know share what's coming up, but he might be very vague. He might be very Reclusive in that sort of thing or if he's an avoidant personality. He's going to avoid questions as well So those needy and avoidant people Oftentimes struggle when it comes to asking questions I want to talk about those emotional grown-ups and let me just say this emotional maturity is weak in both men And women I can share with you that all these questions. I've asked a woman I've had so many occasions. I want to say well over 80 percent of the time The women don't follow up with the same questions with me It's me asking asking asking and they don't violent They don't they're not even inquisitive enough to ask me Juicy delicious deep questions And I got to tell you as a man who values emotional maturity And I mean I truly value emotional maturity that is the biggest turnoff when I've asked a woman a sincere deep question And she hasn't followed up with the same so again Emotionally mature people can lean into what I'm about to share egoic people will only focus on themselves and Avoidant personalities or anxious people are also avoidant as well or they might Ramble the anxious person might ramble on ramble on ramble on but they all care about themselves as well Is this resonating with you? Please let me know by hitting that like button. All right. Let's get into the second one here And that is oh, I love this. What's your greatest professional accomplishment so far? What's your greatest professional compliment so far? Um Oh the sound is bit grainy on my end. However, I can hear and understand you. Okay. Thanks for letting me know I did not know that I've got this new microphone. So I'm hoping that it works. Well Okay, so what's your greatest accomplishment men like to brag About their accomplishments in their professional life. I know for myself one of my greatest accomplishments Is the book that I wrote what the heck is self love anyway? From a professional standpoint what I'm so proud of is this was a number one bestseller on amazon In fact, by the way, if you want to grab a copy, there's a link below right there so I'm asking someone their professional accomplishments men are tend to be more driven by their Professional accomplishments by their career. We tend to identify ourselves with our career So this is a really good question that allows you to build deeper connection And if you follow up like a follow-up question might be jonathan what you do for your living your dating and relationship coach great What inspired you to do that? Remember? I talked about drilling down by asking what inspired someone why they do what they do Is a great question to allow them to elaborate But as I said before Anyone who hyper focuses on talking about themselves and they're not doing the same Oftentimes lacks a sense of emotional maturity because an emotional grown-up understand that communication is a dance It's a back and forth. It's a ping-pong game. It's not one-sided So if you're doing all the question asking now we could chalk it up to someone being nervous But you know what?    Just enjoy the process And lean in with some fun and some curiosity. Let me repeat that lean in with some fun and curiosity Is this sinking in I hope so By the way, the light is making me sweat like you can't believe I will have underarm stains. I am so embarrassed Okay, number three Which uh, do you have a favorite book and why do you have a favorite book and why I love this question Because what a person reads gives you insight into how they think how they operate Um How they view the world they can speak volumes. I'll share with you my dating app for example um, here's my bumble app And I'm sharing this with you because I want to give you some perspective Is one of the pictures on my profile Look at that is a stack of books is a stack of books. Do you see that? All the way from shut up stop lining get a life the uncommon bond the queen's code understand men now Uh chatting or cheating the hoffman process nonviolent communication the untethered soul I I share this because and I get a lot of great compliments on my dating profile because it gives a personal reflection of what am I into being curious and and at finding out what someone reads Speaks volume to their level of depth and growth Speaks level to their depth and growth. So one of my favorite books happens to be where is it? Right here is the untethered soul by michael singer the untethered soul by michael singer And if you ask me why because this book teaches you how to talk to the voices in your head Let me repeat that this book teaches you how to talk to the voices in your head So I really encourage you all to not only get this book, but to ask these deeper questions When you're dating let's go beyond the surface I want to know about atoms and galaxies and and death and aliens and things that make you scared at night. Okay All right, let's get to the fourth one And I said this was going to be a little bit weird because the question is tell me something weird about you Tell me something weird about you now for those who know my work and follow my work Know that I'm a big fan of the movie when harry met sally when harry met sally And sally were played by meg rime was quite unique. In fact, she had a unique way of ordering food It was quite weird, you know everything had to be on the side and she would order in such a way that the chef Didn't even know it was possible But what's so great about Sally and harry is at the end of the movie he says I love it that it takes you an hour and a half to order a ham sandwich. I love it that you think 71 degrees is cold I love it that you're the first person I think of when I wake up in the morning your last person I think of when I go to bed and when I realize I wanted to spend the rest of my life with you I read we're here to tell you ladies and gentlemen. We don't fall in love with the perfection of the person We actually fall in love with those unique cracks in a person those things that make them weird I shared the story often of someone who I cared about deeply that she would always yelp every restaurant before we go out It used to drive me nuts, but when we ended the relationship. I found myself yelping Every restaurant that I went out to because I thought it was weird at first But ultimately that's one of the things I loved about her So ask someone to tell you what's weird. So I'm going to share with everybody something weird about me I invite you to post something weird about yourself whether in the comments or in the live chat But those who know me know I can't stand condiments. I literally am grossed out by condiments primarily for ketchup mustard mayonnaise and relish. I am so grossed out that Now most high-end restaurants don't have them sitting at the table But when you go to a coffee shop type of place, it's there And I literally have to ask the waiter waitress to remove it or I'm going to throw up I am just grossed out by it. So that's something weird about myself. I invite you to share something weird about yourself as well And do so in the live chat or in the comments because it is our weirdness that actually bonds us with another human being Okay, the fifth deep question you can ask a guy to build attraction and I love this one Can you think of the most romantic thing you've ever done for a woman and how did she feel about it? Can you think of the most romantic thing you've done for a woman and how'd she feel about it now? This might send scare some guys But I think this is a great question because if a guy can express His romantic side and what he's done in a past relationship in a positive light You know, sadly when we ask questions about past relationships, you hear such negativity Negativity fact you oftentimes see a lot of blaming. They're always blaming the other person For the demise of the relationship instead of taking ownership and I love this question. What's the most romantic thing? You know, you've ever done for a woman and by the way ladies you can do this Guy can ask you the same question. What's the most romantic thing? But today we're going to ask okay I'm going to ask you to post a comment on what's the most romantic thing a guy has ever done for you Posted in the comments or post it in the live chat. Okay All right, we'll be taking questions in just a second and our sixth and final thing and by the way There are hundreds of deep questions. I just came up with six for today. I'll do another video again And we're going to wrap up with Do you have any fears when it comes to love and relationship? Do you have any fears when it comes to love and relationship? Now you might be wondering while jonathan, how does this build deep attraction or how's this build attraction? This is more about building intimacy when someone can actually share their fears around love and relationships It actually creates an opportunity to bond with another person at a deeper more intimate level So I love this question What's your fears around love and relationship to really expand the greater conversation than what do you like sushi? Me too. Do you like stand-up paddle board my favorite sport? Do you like the rolling stones? Let's go see them in concert. Come on. Let's get beyond the surface here And let's start going deeper when we're meeting people on the second third or fourth date and start going Beyond the surface and really get to know a human being from the inside out Is this sinking in is this resonating with you? Please let me know by hitting that like button All right, so just to recap The six questions deep questions to ask number one What's the one thing you're most passionate about and by the way, there can be way more than one Number two, what's your greatest professional accomplishment? Number three, do you have a favorite book and why? Number four, tell me something weird about you Number five, can you think of the most romantic thing you've ever done for a woman and how'd she feel about it? And lastly, do you have any fears when it comes to love and relationships? And by the way, yes, sushi is a cool thing for those that comment about it All right, we're gonna take questions in a second But before we do I have a tiny little comment to make Really quickly to post a question write the word question and post it buy a super sticker and write the question in the super sticker Or buy a super chat And post the question in there, but it's time for the q&a and yes, I'm amazing I just hit 29 000 subscribers today really quickly before I get into the q&a. I have a little disclaimer My coaching is called heart centered radical honesty It's direct a little tough love and a lot of heart. I think of myself like your big brother Ideally, I wish I could be there for you when a guy is taking you out on a first date So I could be there with the shotgun and say what are your intentions with my sister or the way a father might do that Now really quickly Um Occasionally, I'm not a doctor. I'm not a therapist. There's no certification for what I do after in the course of this If I feel like someone needs medical treatment I actually refer them because I'm a big proponent of seeking medical attention from a therapeutic perspective from a psychotherapy perspective If they have deep wounds, so I'm a big proponent of people going out and doing work Okay, and lastly, I occasionally use Explotives to enhance the sentence and I tend to yell this is not yelling to offend anyone This is just my style of communicating this way It's also much like the way a child is about to touch fire with their finger I'm yelling to encourage everyone to stop doing behavior That doesn't really benefit or serve them. Okay. If this is sinking in, please hit that like button. All right Do we have any questions? Let's take a look at the board Um Please post a question This is the best part. Come on everyone stop being bashful Well, someone's ready to post a question write the word question and the question thereafter I want to share with you two stories One happens to be from a client of mine who got married last year during covid I do see a question that's come up. So I'll grab that in the second leaf. Um She's now happily married to a great guy and I remember during the dating process One of the things she noticed Was this relationship was relatively easy everything about it the conversation flowed easily the way they blended lives together was easy Everything about it was relatively easy. There wasn't a lot of friction And I don't mean that a relationship isn't going to have friction, but it flowed with ease Now I want to share with you a story that I I got a call from a woman this week who is in a Five or six year marriage. She's known her guy for For seven years and she's unhappy and wants to get a divorce And it's been quite frankly She said from the very beginning something was off, but she went against her better judgment There were so many red flags, but she went to get some better judgment Ladies if you're tired of making the wrong choices if you're tired of picking men who are emotionally immature And not ready for a serious deep relationship Then do me a favor check out a link to a free discovery call with me because my area of expertise Is teaching you how to vet for emotionally available men So you don't end up like this person who has a child with them and it's just miserable And she knew it literally on the very first month they were dating But she went against her better judgment. We have to be stronger in our judgments because quite frankly We only get one chance at this You know thing called life. I mean not from a spiritual perspective from from a human perspective Let's make it count. Let's do the best we can can we agree to that? All right I hope that helped. Let me check out. We did get a super sticker So Stephanie. Hey, thank you so much. Stephanie. That's very sweet of you Um, all right, you write how much wood does a woodchuck chuck if a woodchuck could chuck wood Just kidding. Just wanted to send a little cash and love your way since I finally were able to catch you live That is so sweet. She just bought a super sticker for $49.99. Thank you from the bottom of my heart Most and by the way, uh, everyone youtube does take a cut of this But I do say this most uh, my goal with all the super sticker chat and money that come in Is going to go help to create a foundation. I'm working on I'm actually looking into it with an attorney right now for my son Connor who passed away In honor of connor. Um, I'm thinking about calling it salty something I don't know it's got it has to have salty in it because everyone who knows connor knows his nickname was salty So I just want to thank you Stephanie for your generosity. All right. Let's take a look at something leaf writes um Okay, she was writing to someone else Mia writes, how do you tell a guy you've been wanting to date for over a year that you want to date him Love this question. How do you tell a guy you've been wanting to date for over a year that you want to date him? Well, first off, I think let's let's take out the word date And let's replace it with get to know him better from a romantic perspective. How about that? We replace the word dating and replace it with getting to know each other better from a romantic perspective And there's something really simple you can do And you can say something like this. Hey tim Yeah, I really like you and I'd like to take you out for a cup of coffee Are you free Tuesday or thursday at three or are you free at three o'clock or five o'clock? Just ask him out. Why is there why does there have to be so much pressure? Why does it always have to be men have to be doing this legs? Let me tell you something this old narrative that men are are so preconditioned to be the provider and protector and because they tend to be the ones who do the asking for marriage That they're the drivers of the bus. Let me just tell you this Why was the number one search term for most women centered around why are men commitment phobic? Why do men pull away? Why do men you know disappear and ghost?  She said hey, if you asked me out again, I would say yes I mean, so this was on a first date, but at least she gave the indication. She liked me or you could simply say to a guy I like you. I like you That's it. So you have to say now if he doesn't reciprocate in kind He might he might it might you might catch him off guard and that might happen Okay, but guess what if he doesn't reciprocate in kind, that's okay At least you were in your empowered effort and you made the effort yourself. Is this sitting in is this resonating? Please let me know. Okay. Well, I burp, okay mm By the way, Mia, I highly recommend checking out the book if the Buddha dated if the Buddha dated I love this book because it takes out expectations in the dating processes and it replaces it with love Let me repeat that we take out the gender expectation that the penis is supposed to do this and the vagina is supposed to do that Because it's societal rules say that's what's supposed to happen. Let's take out The gender biology and let's get to the heart because at the end of the day our hearts are very much the same Okay, it's red blood that coils goes through our veins and every human wants to connect at an emotional level So let's throw out the expectations and let's focus on the heart and that's my suggestion for you Is that helping? I hope it does. All right And let's see Natasha. Okay. Kelly right. Oh, we got a bunch of questions We fight I'd like to know why YouTube stopped me from being able to get to the final purchase step on any video if anyone knows I don't okay. I don't sorry that um Question can I tweak the the question the most romantic thing you've ever done for a woman? How'd she like it to where? To where it asks the most romantic thing he's done for me and get to say how much I love him for it Okay, so let me just say why I like asking that question It allows a guy to boast a little bit about how he Operates in relationships as it gives them an opportunity and a boast since you're brand new to dating this this deep question to build Attraction is the beginning stage of dating you can't really ask about the most romantic thing He's done for you and I would not ever ask him What's the most romantic thing you would do for me because that's an expectation based way of asking a question So by asking the question the way I laid it out earlier and allows him to boast about how he is as a boyfriend And that's why I like the question Okay, Kelly. Hey, Kelly. Give by the way Kelly. Hi. I just want to say I love your golf swing Kelly writes what if a guy is very vague about his past relationships and will only reveal a few details About a couple of them. Is it healthier to not discuss every relationship in one's past? Okay Now we have two questions here I don't believe you have to talk about every relationship, but let me tell you why it's important to talk about past relationships It's not the content that you're listening for Well, well, let me backtrack it is the it's not about knowing the details of his past It's really asking this question is to pay attention Is he acting like a victim of his past relationships?  It's just very hot Okay, if he gets defensive if he tries to avoid talking about our past relationships is a great indicator For how emotionally mature a person is not so much by the the details of their past relationship But how have they processed and how have they healed? So if someone had a traumatic past relationship, everyone, please write this down if you're watching the replays pause this and write this down How did you heal from that past relationship or what did you learn about yourself in that past relationship? These are very telling questions to ask a guide This builds deeper connection for the emotionally mature guy and for the emotionally immature guy He is going to run as fast as he can for the avoidant man He's going to run away from these questions And why you want him to run away because if a guy runs away, he can't lean into deeper intimacy If a person can't show up as an emotional grown-up in relationship They these people become very problematic just like that that woman I shared with you Who called me up frantically? Because she's happy in her marriage and she said literally that answers were all there because he basically operated from victim consciousness Here in the united states. We are suckling on the nipple of victim consciousness We are suckling at it so many human beings don't take ownership for their part So why asking questions and if someone deflects or gets angry upset That's not a red flag. That's a deal breaker one as fast as you can because that person is going to be a problematic person To be in relationship. Is this sinking in is this resonating? Please let me know by hitting that like button and hit the like buttons, please Okay, um, thank you kelly for your question.  I can't even think of how to pronounce the last name Well, we'll just say who did sure julia roberts plays shelby in what movie? Let's see who gets that right in the movie trivia question. All right. Let's get natash's question now Question hanging out with a 50 year old guy. I'm 46 for six weeks one or two times per week He compliments me helpful great conversation cares about my feeling yet still no kisses Have been tactile and smiles I don't know what's hating natasha then then smack a kiss on his lips because I I know that i'm trying to kiss a woman on the first date. I mean if i'm attracted to a person I'm trying or at least I want some a little kiss at the end of the first date But certainly if we haven't gotten to the second date and we haven't kissed Um, maybe this guy doesn't have much of a libido or sex drive. I don't know But if you want to if you want to kiss smack a lip Smack your lips on his lips and kiss them and then see what happens because most people will tell you They can tell a lot about a person from a first kiss So to me that's a very important thing six weeks in 12 times being together you should be kissing. Um Or it's just a platonic relationship and maybe that's what it is to him. Maybe he sees it as a uh platonic relationship So by initiating you can get a better sense of what's going to happen or at least initiate a conversation and talk about it Ladies we have to stop being afraid to have conversations wins with men. This Rosenberg. Please get this book This is such an important book to get to learn. It should be called command compassionate communication And that's what I want to encourage everyone to do is to have more compassionate communication all right, um Let's see. I thought I saw another sticker, but I guess not Yes, the answer is steel magnolia is not mystic pizza. It was steel magnolias Okay, if you have a question write the word question or buy a super sticker and a super chat and I'd be so grateful Okay, kelly says very helpful answer. Thank you working on the swing. Yes, Heather for a few hours He has not been intimate informed. Okay. Let me clarify pandemonium. Are you saying? The connection is off. I don't All right, it looks like we're having problems. I guess we're going to have to wrap up today everyone Thank you so much for joining in my live stream today. Thank you so much for joining in my live stream today I hope you find value in the content that I share Um, this is one of my pros in it a little bit crass at times I yell at times, but it's all just to wake people up because my goal is to shift perspective First and foremost to have everybody love on themselves. I'm a big proponent of self love because when you love on yourself When you love on yourself, it doesn't matter whether or not you're in a happy relationship with a man because the most important relationship You'll ever have is the relationship with yourself. And that's what I want to encourage everyone to be again I'm sorry for the technical difficulties. I'm hoping that this will be fixed next time in the meantime I'm going to wrap this up live stream as I always do first off giving myself a big


Abstract Alzheimer's disease, AD, is a progressive neurodegenerative disorder that affects millions worldwide. Despite recent advancements in research, there is currently no effective treatment available. This paper proposes a new approach to treating AD by using a nanoparticle drug delivery system called Transfer and Receptor Zinc RNA-aptamer, TR-ZRA. The system is designed to target the blood-brain barrier and deliver therapeutics to the brain. It is loaded with two components, a CD-22SHRNA plasmid to reduce the production of a protein associated with AD and A-beta aptomers to detect A-beta plaque levels. TR-ZRA was tested in mouse models of AD and found to improve cognitive function and reduce inflammation. Additionally, it was shown to increase the ability of microglia to clear A-beta from the brain. These results suggest that TR-ZRA could be used as a potential treatment for AD. This article was authored by Yanrong Su, Yufan Huang, Qin Jekou, and others. We are article.tv, links in the description below.


So, thanks to the organizers, first of all, and the judges for giving me the chance to talk about some of my work that I've been doing during my PhD. This is a side project, so I made a project when I was doing this stuff when I had some time and not doing making pasta, so when I was not making pasta, I was doing this. So, starting with this picture, which is the same picture that Randy Cummins showed in his talk, and this picture shows it was taken in the 1970s, and it's the Kinetoplast DNA, so outside is a natural environment, which is the mitochondrion of some organisms of the class Kinetoplastida, and what's peculiar about this structure is that it's fairly unique in nature, so these are all small rings, there are links together, and they are all packed inside this disc-like object, membrane, in the mitochondrion, and when they are taken out of from there, they look like that, so they're all linked together. So, in the 1990s, the group of Cossarelli started to think about this problem from a more mathematical and topological point of view, and they suggested that maybe this network is made of, like, you can think about this network as a two-dimensional layer, where you have rings linked together in this fashion, and you can check that by doing digestion experiments, so you put the restriction enzymes in the solution, you break down the network and you see the products, and you can check whether your model fits the data, and I don't need to tell you that this structure really reminds Olympic gels that were imagined by the gents, and as far as I know, no one has made an Olympic gel, a synthetic Olympic gel in the lab up to now, but nature has been doing it for thousands and thousands of years, so maybe we can learn from nature, also in this case, try to make something smarter. So, I started from that picture, so my work was motivated by the fact that the Kinetoplast seems to be biologically successful, but how can we understand that? I mean, can we understand something better, more destruction? So, what I know from that picture is that the DNA is all packed under strong confinement in that disk shape object, and so what these people have been doing, some of them are in the audience, actually, so it was to model the network as two-dimensional layers where rings were allowed to link with one another. What I did was to relax this assumption and to simply model, do some brand dynamic simulations of rings that were allowed to pass through one another, and then from there you freeze the network at some point, you compute the pairwise linking number, you get the network out of that, and from the network you can compute whatever you like, for instance, you can compute the mean balance of the network, that probably the distribution of the linking number, and then what you can do, you can simulate, you can do the simulations of this restriction, digesting experiments, and you can do it for different system densities, and then you see that this value of density and the products that you get, the relative abundance of the products, they match pretty well the data from the Kostarelli group, so then we went back and then said, well, what about that density, what's special about it, and then we discovered that density corresponds to this point in this graph which shows the size of the largest connected component in the network, so what it means is that it's surprising that it's not either down there or down up here, it's right bang in the middle where the percolation is start to take place, so what we argued is that the network is near the percolation point, and then you can imagine the kinetoplast as being optimizing the linkedness of the network, so it's neither, and this is biologically important because clearly you want to keep all the genetic material together when you divide, but then you also want to do the replication quite quickly, so you don't want to unlink, you don't want to be super linked because otherwise you'll have to do too many operations, and so I don't have time to go through this part of the talk, okay, so a very cool experiment that I want to just to mention briefly is this one, which is so how the replication, so even Kamin was saying how does the replication occur, because if they are all linked when they divide, so the point is that basically the network seems to rotate around two fixed points, and then the mini circles get unlinked from the network, and then re-linked at the periphery of the network, and you see here the tagged newly produced mini circles that are introduced from the periphery, and they diffuse inside the network, that was pretty cool I thought, and we tried to come up with some simple model, and we are working on it, and here I end up thanking collaborators and people having the honor to talk to Ingorek, Gareth, and Tom, and Matthew, I remember so, and Edinburgh, Fundings, HIP, PSSC, and this is some linked pasta, I want to do the whole Kinetoplast, so then I go quite lazy and I stopped at two, so. Okay, time for a quick question, I've got one, the Kinetoplast network has maxi circles in it too, did you put any of those in? No, they are biologically important, that is for sure, but we haven't taken them into account in the model. Okay, well thank you. Ah, that's a good question, we haven't checked the distribution, I haven't checked the distribution, I don't seem to have, but the average variance in history, this is a good point, I should look at it. Okay, let's thank our speaker again, the next talk is by Wandan in Piscaya from Warsaw, talking about complex lasso topology in proteins. Okay, good morning, I feel very honored and quite surprised to find myself on that side of the audience today, I'm a mathematician who work with some theoretical staff, functional equations, and so on, and I was very lucky three years ago to meet Joanna Słukowska, and because of her to meet all of your society, work with Ken, collaborate with Eric and with the others, and to get to know a little bit of your beautiful and colorful science, I'm very grateful for that today. And this is my poster, which is kind of a picture of last two years of my life, of my adventure with Joanna, which resulted in those free databases and servers on the complex topology in proteins, so Joanna showed all of them in the morning today, I will try briefly to encourage you to use them. So, not protein is the first one, which collects all the information about the nuts in the proteins, so all the statistics from whole PDB and the detailed information about each particular structure, and what's the most important, we also have a server for you where you can upload any structure, actually it doesn't have to be protein structure, and you can get all the information, and in case of any questions or any problems with the user server, please contact me, and then the second one is lasso abroad, and Joanna told you a little bit about lasso, so lasso appearing in the protein when we have, in any structure, when we have some additional bond that creates closed loop, so we have closed loop, and then the tail can go through the loop, can go, can go around or can do whatever it wants, so those are the lasso types that we have found in proteins in the PDB, and in this case we also have a server for you, for instance you can upload the trajectory, you can choose some options, more detailed or less detailed calculations and many other options, some advanced options, and in the case of trajectory you can get such results, so there is no time to explain you in the details, but I just want you to make me believe that from this picture you can very easily see that in this case we had some loop, and the tail went through the loop first crossing the loop in two places, and those two lines show us that there were two places, and then it went totally through the loop, so anyway you can really see from those information the pattern of folding the protein, and you can also see the detailed information about any frame, any structure, and also in any question, in the case of any questions, problems, this can take me, and then the last one, link prot, is from this summer, it's very very very fresh and very tasty, hopeful, so for two chains, two open chains, we close them on the on the spur, so we obtain few link types for any structure with some probabilities, and what kind of links we have found in proteins, we were looking for the links between up to four chains, so with the probability higher than 50%, we found two links for two components, only for two components, hopp and solomon, for the probability 30%, we found star of David as well, and two links between three components, and for the probability 10%, we found a couple more, that one is the most complicated for two components, has 10 crossings, and as I told we checked also all the set of all four, all the sets of four components for chains, and those are link types that we found in proteins, with the probability higher than 10%, and this part of our project was kind of exciting, because those structures, they aren't in any tables, link tables, not tables, as far as I know, so that was kind of exploring, kind of exploring new things, okay, and here we also have server for you, and in case of any questions and problems, please contact me, those servers and databases are made for you, and I hope that we can together move on with some new discoveries with those other things, thank you. Okay, quick questions. Well, I'm going to stop by, I think my question's unfair. I'm just, I want you to speculate about, kind of looking forward about our ability to design knots, any knots, with protein chains in general, knowing what we do about all of this, how easy, hard, what do you think the challenges might be? Perhaps that's unfair. So if you're really interested in my opinion about that, yes, you know, I'm not a specialist in those, in this thing what you asked about, but what is exciting for me with that collaboration, that they really, what I can see here, I really believe that those things are possible, and that's what I, I think that the thing that you asked, it's not easy, but I hope I will see in my life then, yeah. Okay, let's thank our speaker once again. Our next talk is by Antonio Sumo from CISA in Triesta, poor translocation of knotted chains. I would like to thank the organizer for choosing my poster. I'm going to talk about my work during my during my PhD under supervision of Christian, which is on Portland's location knotted chains. So the motivation of our work is the ongoing effort to sequence DNA. In this case, sequence single strand DNA making them pass through nanopore. This sequencing experiment are involving longer and longer chain, which have high probability of knotting. So we asked ourselves what can be the impact of the presence of knot inside the chain when it starts to translocate. So our model is a really a basic flexible chain, which is mapped on the single strand DNA, but can be regarded as a zero model for other biopolymers like proteins because you have some charge interaction, but it's really screened. So the first work was done by our group in 2012 and shows that microscopic intuition or friction is how different practical one. So I'll tell you the translocation is going to follow. So the tension from reach the pore that is located here in this case in the green region, then the knot tightens and drags toward the pore and here it gets stuck because we choose a pore small enough to make all the single strand pass. So while our intuition phase, you can see that in the case of low forces, when they're not reached the pore, the translocation can still continue to go on until the end. While for high forces, this is not the case. As you can see, it gets stuck. So we let's say recover our intuition. So we started from this observation, let's say this phenomena and try to understand better, which is the impact of the other different topologies, most simple one, let's say, and even some composite knot. Here is the basic phenomenology. So you have the velocity to translocate 30% of the chain for different topologies and different color representing increasing force. So in that not that case, yeah, that when you increase the force, the velocity is just proportional. Well, if you, let's say, select here, twist knot, you have what I showed before. So for small forces, the translocation can go on. Like for high forces, it jumps. This is the case for our twist knot. Instead, if you look at the twist knot, the situation is different. So yeah, the translocation can go on. Anyway, for these cases, you can see that the scale of the velocity is different from the, they're not the one. So in any case, we have some impact in translocation. Another nice feature is that if you have composite knot and change the order in which they arrive toward the pole, you have that the velocity is different, as you can see. So if you want a better explanation, you can come later to my poster in the last minute. Here, I'll tell you briefly how we characterize this phenomenology. So in particular, we measure the tractive force before and past the knot, because you can see the knot as a dissipative machine, for which an applied force, an output force, which is a smaller one, we measure this output versus input force, and you can see that for the twist knot, you have the force decreased to zero, where for torus knot, it continues to increase. So basically, the difference between torus and twist knot is that torus knot have just one single tracking points, while twist knot have at least two main tracking points, one at the entrance and one at the end, meaning that the friction is different. This plot also can let you understand where composite knot be in a different way if you change the order. So you can see composite knot as the sum of sample knot, in which cases you can compute this force before the second knot using this diagram. So actually, changing the order means that you compose these curves in different ways, meaning that of course you're going to have different results. And this concludes my presentation. Thank you. Okay, time for a quick question or two. Nice talk, thanks. Maybe I just missed it, but I didn't understand how you applied the force. Okay, yeah, no, I didn't tell you because it was too short. So the force is applied just inside the pore, as done in experiment. So it just applied, let's say, an electric field inside, and it starts to translocate. So does every bead fill a force or just one bead is the force transmitted along the chain? No, no, no. Okay, the force is applied just inside the pore, but of course it transmits along the chain. There's a lot of paper solution on this. So yeah, the force is transmitted toward the end of the chain where we're doing it. It started to rectify during it. Great. Thanks. One more question. Let's thank our speakers again. On behalf of the organizers, there's some very special people we'd like to thank. One is our technician, Iqbal. So give him a hand. But a very, very special thanks for our secretary, Erika Sanataro. Erika, you've done a great job. And on the behalf of the organizers, thank you for coming. This was a terrific conference, and you made it happen. So thank you.


How's it going everybody forced here we're ribbon 2022 of pristine baseball four-box break Number 28 it's a random team style. I'll let you guys take a look at the results there You can always find them at late sports cards.com here. We go everybody. Good luck Jram the hero for Cleveland the Phillies stole one from the Cardinals man At that magic crazy day so far love playoff baseball the only issue of playoff baseball as it goes by so fast So you've just got elements a prism first off the line one boxer for football and a prism hobby three box for football as well guys That is it for tonight. Persons are close to full as well Again, I'm gonna say it until it expires use that coupon YouTube members the code is in the Community tab on YouTube you want to join YouTube membership go to the membership tab. It's only 199 a month Get all the cool emotes sometimes you remember the only chat, but you're always the other chat with The membership and then periodic coupons like the one right now also exclusive content Get heads up for when like all my acts go on sale and things like that. So you get show recaps You're gonna see singles that we buy it shows singles that we sell it shows This little bit of that you guys All right, it looks like the Mariners are gonna hang on for this one. We'll see though We'll see you can see what happens. Who do we got tonight? Got Padres with Darvish or the Mets for sure. I mean, that's a pictures do right there It's gonna be I'm predicting a lot of strikeouts in that game That's a that's a good old-fashioned game one pictures dual For sure had a lot of those in every single game today pretty much actually for sure For sure sir. Yeah, it sounds like sounds like Sean's going with the Mets. Oh I don't mind the Mets I guess go Padres each row pristine borders, but I like to see the Padres I Wouldn't mind the Mets but I guess the Padres won't Padres just because the Mets have been tortured They've been tortured These are all basepacks here you got pristine borders Pedro Red Sox, but I think I think I mean the Mariners are America's team right now all day They should be the world's team man. Go Mariners pure power Raphael Devers Red Sox. What up, Cory? What's going on Cory? Okay, come on pack Come on now slamming Sammy pristine borders Oh We're running it Mariano Rivera pristine borders refractors now Gary Sheffield is a Marlin purple refractor a's Matt Olson 42 out of 99 Areas right there. There's the grom. He should be going game to Purple refractor a rod rangers 74 out of 99 Kirby Puckett Riley Veerling and a gold Jersey auto white Sox Bo Jackson White Sox Bo Jackson 45 of 50 Heck yeah, non-zeo. 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You got a patiently wait on that one Dang, man.  He sticks all the time in postseason strikeouts.  He's the youngest Player in Mets postseason history Francisco Alvarez, he was the number one prospect this year to Tyler McGill fresh faces Mets rookie So tug Lori, how's it going? Mookie pure power That's the thing about the Dodgers because I'm a Red Sox fan. I love Mookie. So like when the Dodgers do well I do not mind it at all Got a Beltray pristine Borders Rangers. I Cannot be upset seeing Mookie Betts win things and do good can't be upset pure power Aaron judge Is that guy have a lot of power really? I mean I guess right and then Miguel Cabrera pristine Borders Fractures now with that dummy thick card coming up Joe Barlow rookie got Ryan Sandberg Cubs orange nice one gets on the numbers in this break 22 at 25 How see your proper refractor Red Sox JD Martinez 15 of 99. All right. Here's that dummy thick card It's an orange. So it's gonna be a relic auto orange White Sox Probably Frank Thomas, right? Yeah, Frank Thomas sick hit Jersey auto orange 13 at 25 white Sox the big hurts White Sox crushed in the break man had that awesome Bo Jackson earlier as well and Frasier purple refractor Brandon Lowe Tampa Bay Number 84 and 99 think I had some cards Gold refractor Max Munty Dodgers 38 of 50 Louise Robert and Ozzie Smith Jake still here Gold refractor twins Kirby Puckett 18 of 50 nice twins card their blue refractor Ozzie Smith Cardinals 45 is 75 raised based autopilot glass now The refractor auto Phillies Reese Hoskins 80 of 99 Rookie refractor and main warfare Royals not numbered and a base refractors. He asked her hand as blue jays last box Anybody play overwatch in chat. I think I'm gonna play some overwatch to tomorrow for the first time I haven't played it came out Tuesday. I haven't played it yet Yeah, I'm the same way soon. It's been too busy to Because I know when I sit down to play it. I want like a few hours to You know, I can't just dip my toes in the water with overwatch. I gotta go All in, you know Got a cannonball. I played the beta a bit. It was good. I do not know if the Q time issue was solved I have no idea Work on my gamer hours. No, man. I got lots of breaker hours. I don't have a ton of time to game I'll run up by rich I'm like rich instead of me breaking one day Do you want to just pay me to play video games for one? That he'll totally be on board with that Yeah, I'll play ranks Probably not at first, but I gotta get I haven't played the first one in a while I played a lot of ranked at first. I was never really that good. Yeah, that's Invicta. I I help pace Invicta. I'm a subscriber Got a pristine borders of Cunha. So Victor always plays video games on my dime, huh? It's crazy, man Like some one sub does barely anything a pristine borders Francisco Lindore Or the Mets I'm just kidding sin. You know, I'm kidding It's your month anniversary Where are we going to dinner?  Yes, sir pristine borders. Jose. I pray you Shot subs to you. I've given I've given a lot of subs in cinch tap before I haven't been in there for in a while though I mean, I'm sorry. I pop in the window. I gotta get back in there Sin Vic is always going live. I like where we're one a.m. Dude Also, I just am busier lately, I think pure power Reggie Jackson. Yeah, I don't know. I didn't see it Appreciate it. 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That's a nice card 7 to 25 angels and a red refractor Bryce Harper one of five bills That's gonna do it. We've got museum collection coming up. Thanks again everybody