And she sold this for $90. She picked it up at a consignment sale for $8. And that was on Facebook. Hey, Bolo Buddies, thanks for watching. All right, you guys, I have 42 Bolo's coming your way, items to be on the lookout for, items you can hopefully buy low and sell high. These come from featured Bolo Buddies members and I've got 14 members that I'm gonna share with you, three Bolo's from each. So get ready because there are some big money Bolo's in here. I'm gonna tell you where they got the item, what they paid for it and what it sold for. So let's get started. Hey, Bolo Buddies, thanks for watching. Online Little Pony, amazing, right? All right, let's get started. So the first item comes from Philly Flipper. If you have not checked out his channel, I highly recommend it. He is almost to 9,000 subscribers. He has a really unique way of showing his, like when he goes out to the yard sales and to the auction houses, he takes you on the journey with him. But he also puts in edits and pops up how things are listed and when they sell. So it's definitely educational. So check him out for Bolo items. The first item he sold is the stained glass Tiffany mushroom shape lamp. And he sold this on Mercari for $150 and the buyer paid shipping. And this sold in less than a week and he paid $10 for it. The next item are these tailor made, oh, what do you call them, golf clubs. And he picked these up at a yard sale for $15, took two days to sell and he sold them for $170 plus shipping. The next item is this vintage new old stock with the original box perfume, Levin, or Lanvin Spanish geranium mist, fancy. And he paid $90 for 50 bottles of perfume. So about $1.80 for this. And this one sold for a best offer of $100. The next item comes from a rural squirrel. She does a ton of Benz videos and she does a lot of challenges where she goes and meets up with other YouTubers and they are super fun videos. Definitely go check her out if you have not already. She is linked down below along with everyone else that I'm gonna mention here in this video. All right, she picked up these at Ross. Ross is a discount store, kind of like TJ Maxx or Gabriel Brothers, just a discount store. And she paid $29.99 for these and she sold them on best offer for $140 plus shipping. So Doc Martens can definitely be a Bolo, whether they're new or old, they seem to do pretty well. So Ross, you guys, is selling Doc Martens. Check that out. This next item is from, she was at the Goodwill Benz and she picked up a whole bunch of these, she said. One of them sold for $75. This one, she took a best offer of $44 and she said, by weight, it cost her about 45 cents and the buyer paid shipping on this. Now, if this here is marker, what you can do is take a dry erase marker and if you go over that, just rub over it and then take a paper towel, that will come off. So that is just a little tip, if you guys haven't seen that on my channel before. So dry erase marker, it's like magic. The next item is a Christian Kota Kata. I don't know, it's a skirt. She said it's 100% silk and she picked this up at the Goodwill Benz for $1.14 and she took a best offer of $72 plus shipping on this. Here is the tag and that is a fancy, fancy skirt. Dug it out of the Benz. Isn't that amazing? The next item comes from Leo's Treasure. She does not have a YouTube channel but this is her eBay store. Definitely go check her out and see some of the different items that she has available. Definitely some cool stuff here. All right. And the eBay stores are linked down below also for all of the sellers in this video. So this is a beer tap. She bought 20, or I'm sorry, a box of nine for $20. I think I'm reading this right. And it ended up being about 75 cents per tap. So I must have written down a number wrong. So if it was 75 cents and she sold this for $79.96, it's a sweetwater fish trout beer tap. Look how pretty it is. Wow, that's cool, right? There's the markings on it. And I don't know if she got that at an auction or where she got it. The next item is this Gund 1982 pink bunny rabbit. Hard to find. HTF stands for hard to find. She got this at a thrift store for $2.99 and she sold it for a best offer of $35.75 and the buyer paid shipping on that. The next item, she took these off of a men's blazer. It was a chaps by Ralph Lauren blazer that she picked up at the Goodwill for $2.99 and she scrapped the blazer because of the condition but she pulled the buttons off and sold them. So pretty cool. She sold those for $32.75 plus shipping. The next item comes from Double Tease Treasures and this is her YouTube channel. Definitely go over and sub her up. She does little collab videos with other YouTubers as well. Lots of great information over here, how to clean without chemicals, different, just different stuff. So check her out. And she sold, I picked this one from her store. I was like, you got to tell me about this Chef Boyardee and she's like, well, some of the stores can't get it. So she listed them on eBay and I think she sold like four or five of them but she paid $2.98 each for them. So a total of $18 in these and she sold them for $77.97 with free shipping. So not huge profits but she sold four or five of them and that's pretty easy to pick up and ship. So pretty interesting and she got it retail arbitrage from Walmart. The next item is these Lily Pulitzer Men's Shorts. So we all know that Lily Pulitzer women's items do well. These are shorts and she got these at a thrift store for $6, took a best offer of 50 and the buyer paid shipping on those. These are Jinko, J-N-C-O and they usually have some sort of patch or embellishment on them. These don't have, they're not too fancy. They've got the little patches there as seen but these are, I'm gonna say they're size 11 so I'm pretty sure that's a junior size and they're kind of a skater style. She got these for $6 at the Goodwill and she sold them for $122.96 with free shipping. The next item comes from It's All Goods 94 and this is her, she doesn't have a store so when you click on the link it's just gonna take you to a list of her items like I'm showing you here. So definitely go check her out. She's got some really cool stuff. She does a lot of toys but it looks like she's got some lava lamps listed so that's pretty cool. I would not wanna ship a framed and she got this for $2 at a thrift store and sold it for $210 plus shipping. The next item is this Lionel Heavy Duty Construction Zone Electric Train and this one came from a yard sale. She paid $10 for it and she sold it for a best offer of $89. Let's see. So it looks like they're just plastic trains but they are Lionel so that's a pretty popular train brand there. This one's pretty cool. Plato Cookie Monster's Letter Lunch Alphabet Sesame Street and it says see details. So I'm guessing if it's older that the Plato might be dried out. I don't know. Let's see. She got this at Savers which is a thrift store for four bucks and took a best offer of $50 plus shipping. The next item comes from the Funky Pickle Thrifter. Oh my goodness. She's so close to 4,000 subscribers. I'm guessing she's gonna have hit it by the time I released this video but you all definitely need to go sub her up. Look at this finding uranium glass in my vintage jewelry. How cool is that? Did you guys know that with a black light some jewelry glows? Check that out. All right. She sold and she finds crazy stuff at garage sales. She got this at a garage sale for $2. It is a Opal Cluster 14 karat gold necklace and she sold this for a best offer of $390 and the buyer paid shipping. She also sold this vintage Dave Clark doll. It is a collectible and it's, let's see. Trying to see here the brand. Oh, Remco. And she got this at a yard sale for a buck and she sold it for $69.99 plus shipping. So she specializes in jewelry but she also sells a lot of really cool vintage items. These underwear she picked up at a junk store. I'm not sure what a junk store is but I've heard other people call places they go junk stores also. She got these for a dollar each. I think she said she bought about 10 different packs and she sold these for $27.99 each. It looks like she had three of this exact pair and so a dollar into $27.99 three times is pretty, pretty good. This next item comes from Butterfly Hustles and again another awesome YouTube channel. You guys, these YouTube channels are amazing. Go check them out. You will learn so much and she does a lot of thrift with me videos and super, super knowledgeable and an awesome Bolo finder. So what did she find? She found this Johnny was tunic top at the Goodwill for $4.49 and she sold that for $180.40. Let me see if I can find you a tag here. Here's the inner tag of the shirt. So it's embroidered, looks like that. She sold this vintage WF Washington Forge flatware. She got this at the Goodwill for $5.50 and she sold it for $76.50 plus shipping. And the last item she sold is this vintage 1940s Girls Doll Ice Skating Winter Outfit and she got this for free and took a best offer of $43 with free shipping. The next item comes from Vintage Barbie and she just did this video and it has over a thousand views already and she is brand new to YouTube. So that is awesome. Super excited for her there and definitely goes sub her up. She's going to a state sales and taking you guys along with her and doing a fantastic job. So what did she sell? She sold these Vintage Panties that she picked up in one of her recent videos. So check that out. Let's see, she got these for a bucket in a state sale and sold them for $26 on auction plus shipping. The next item she sold is this Fairy Doll Gazelle Catherine's Creation. It's a 33 inch doll with wings. So it is really, really a big doll there. Look at that, that is so neat. Wow, $15 at an auction and she sold it for $51 plus shipping. And the next item she sold is this Dungeons and Dragons. I think it's the game. It's got dice and papers. She got this at an estate sale and it's in her most recent video. She paid three bucks for it and sold it for $125 plus shipping. So that's fantastic. The next item comes from the Rebel reseller and her and I just did a collaboration video where we purchased a $50 plush lot from Donna Telebotelino. So definitely go check out and see what she got and check out what I got and see who you think had the better box or bag. Pretty fun collaboration and I really appreciate that she did that with me and super excited to do more things like that in the future. All right, so she is a Bolo Finder. She sells tons of plush. She is like a plush expert. Okay, so she sells tons of plush but she also sells toys and other vintage items as well. So she is definitely a Bolo Finder. Definitely sub up her channel, the Lion King battle fighting action figures. They're just action figures. Come on, are you kidding me? I think I've sold these before but I think what is really helping this is that it's got all of the animals there and she sold this for $90. She picked it up at a consignment sale for $8 and that was on Facebook. This Etsy sale, she sold this on Etsy. It's a Tyco Kitty Kitty kittens tiger plush and I'm looking at how beat up hers is and I'm thinking the one that I sold for $35 I should have just done a buy it now on it. I don't know if you guys saw that video but I found one at a thrift store and a bunch of plush and it was really, really ratty. Just in rough condition but this one really is not in, it might be in a little better condition and maybe this one's more rare but or harder to find. But yeah, I ended up selling mine for $35. So in hindsight I should have just put a buy it now or best offer and or maybe did an auction and started it higher. So if you see these, they are collectible, highly collectible, people want them and they will pay up for them. This sold for $71.95 plus shipping on Etsy. And the next item here is this strawberry shortcake mini figures lot from the 1980s. She got these at an estate sale and a lot of toys. So her cost of goods was about a buck and she sold this for $44.95 plus shipping and the Kitty Kitty Kitty came from the Goodwill Bins. The next item is from Yard, Sales and Dreams. Again, another awesome channel. Go check out this and Pony that she sold. Oh my goodness, you may have seen it on my channel but wow, just an awesome find there. All right, what did she sell?  She sold this Frederick Remington bronze sculpture. She paid $100 for this. So she knew it was a bow low and she paid up for it. It ended up selling for $399.99 and the buyer paid shipping on that. The next item she sold is this beautiful, amazing painting and she said that the buyer requested that they take it out of this awesome frame and roll it up and ship it to them. I think the frame is just amazing. I love this, I love it. She got this at an estate sale for $8, took a best offer of 170 plus shipping. The next item here is this tanker. It's a 1979 Precision Products wheeler, 18 wheeler truck trailer remote control. She got this at a storage unit pickup and storage unit pick, I'm sorry, paid $5 for it and sold it for $380 plus shipping. The next item here comes from Lake Threads. He doesn't have any content yet, but he is looking to do some in the future, so check him out. And what did he sell? He sold this Orvis Carry It All fly fishing bag, new without tags. He got this at a thrift store. He did pay up for it, he paid $40 for it, but he sold it for $135 plus shipping. The next item is this Disney Trader Sam's 2021 Jungle Cruise Boat Dole Whip Disney Bowl with Oar Spoon. So he actually purchased three of these. He paid $1.70 each, one sold for $31.50, another sold for $39.99, and he has one available and the buyer paid shipping on those. The next item are these Hoka. I think that's how you pronounce it, they are tennis shoes. And these are pretty popular from what I understand I have never sold any. I don't do a lot of shoes in my store, but I do know about this brand. Oh, what's he? Oh, it's the other shoe. I was like, what is that? Thought maybe it had something attached to the bottom. He paid 15 for these at a thrift store and he sold them for $55.99. The next item comes from Gail's New and Vintage and she does not have an eBay store, but you can find her down in the description. I have a link that will take you here and then you can kind of just scroll through her other listed items like I'm doing right here. Now, this first item she sold, she sold on Poshmark, so she also sells over there. It's a NASCAR M&M jacket. And she sold this for $150.  She sold this Sega Sonic the Hedgehog. It's a little three inch figure toy. She got it for 10 cents at a yard sale and sold it for $37.79 plus shipping. Now that is my kind of Bolo. I love toy Bolo's. All right, and this Trifori Vintage Loosite and Liquid Silver Tone Modernist Necklace. Free from her friend and she sold this for a best offer of $60 plus shipping. The next item comes from Gigi's Goldmine and this is his store. It is also linked down in the description. No YouTube channel, but really, really cool stuff here. So definitely go and check him out. What did he sell? He sold this Frankenstein. He got this at a garage sale for $1.50. What a steal of a deal there. It's 36 inches. Those blow molds can definitely be big money. They are kind of a pain to ship in my opinion, but people do it all the time. I just don't like shipping big stuff. He sold this for $110 plus shipping. The next item he pulled out of a mystery box, a mystery toy box from Auctions For You. Her YouTube channel will be linked down below as well. If you're looking for inventory, her and Donna Telebotilino sell inventory to resellers. So definitely check them out, linked below. This one, he said, by the time he unpackaged everything and divided it out, he had about $5 in this and it sold for $129.99. Batman the Killing Joke Graphic Novel Collector Set. So that is a nice Bolo. And I featured another item that he got that was from Auctions For You also. So he must have had a really awesome box. Taco Bell Plush Chihuahua 1998 dog. It's an advertising dog. He got this at a garage sale for $3 and sold it for $24.99 plus shipping. The next item comes from One Stop Shop 59. Oh my goodness, look at that vase. Ah, it's an owl that is so cute. If you don't know, I love owls. So she has a really, really cool store, lots of really unique items in her store, collectibles, interesting stuff like that. So definitely go check her out. The next item, okay, Anna-Lee's Sass in Class Hot Water Cornbread Cookie Jar Small. She got this at a thrift store for $3 and sold it for $47.81 plus shipping. So it's a cookie jar. Rare Rock America Alex Pardee Bunny with Mint. I don't know what that means. I'm trying to figure out what on earth this is. Is it just a plush? Huh, I don't see the tag. Here it is. Bunny with, Bunny with, huh. Oh, she's probably saying, okay, Bunny with is the name. And then she's saying Mint with tag from 2004. She got this for $0.25 at an estate sale and it sold for $28.87. Okay, this next one she said she picked up at an estate sale for $10. She held off on listing it for a while because she was afraid it would get pulled. I'm covering up, it's nude and she is well endowed. So I don't know what the rules are for wood sculptures being nude. I know a lot of anything else that's nude has to go like in a separate, I think there's like an adult section of eBay. I don't know, I don't sell in that section. So I don't know too much about it, but it did not get taken down because of it. Maybe because it's a wood sculpture. I don't know if anybody has any information on that, please let us know in the comments because I'm not real sure, but I'm gonna cover it up. So because it is, yeah, it's a lot. Anyway, she got this for $10 and she ended up selling it for a best offer of $114.50 plus shipping. So it's a beautiful wood carving, but definitely nudity. So not sure how that falls into the guidelines of eBay, but definitely before you list something, whether it be a wood sculpture or a sculpture in general or I don't know, like I know photographs that are nude and I think magazines, that stuff cannot be put on eBay. At least not on regular eBay. So look into that before you list, but definitely some really cool Bolo items in this video. Big money Bolo's, what was your favorite Bolo? Let me know down in the comments. If you made it this far, put a hat emoji. And if you're new to the channel, thanks so much for being here. If you've been here a long time, thank you so much for being here. I appreciate every single one of you. Be sure to like, comment, subscribe and share. And as I mentioned, these are members of my Bolo Buddies YouTube channel. This channel, if you would like to join memberships to be in a video like this, you can join at level two. It's $4.99 a month and that gets you in this video. If not, no worries, my channel is completely free. This is just for people that want the perks of getting a shout out and getting their Bolo shared. So thank you so much for being here and I'll see you at the next one. Thanks for watching.


Live from the Moscone Convention Center in San Francisco, California, it's theCUBE at Oracle Open World 2014. Brought to you by headline sponsor Cisco Systems with support from NetApp. And now here are your hosts, John Furrier and Jeff Frick. Okay, welcome back everyone here live in San Francisco for Oracle Open World 2014. It's theCUBE. We go out to the events. Extract the city from the noise. We're live on the floor inside the Cisco booth. We also have another CUBE broadcasting live as well. Double the trouble, Jeff Frick, my co-host here inside theCUBE. I'm John Furrier, co-host. Our next guest is Raghunath, an NVR distinguished engineer, data center business group at Cisco. Welcome back to theCUBE. Great to see you again. Thanks John. Now this is pretty exciting. I can't wait to hear that 40,000 plus attendees in San Francisco this week. Yeah. Oracle is chouting benchmarks. I love Larry Allison, Jeff, when he gets on stage. We are the fastest. Like he's always like, whether they're the fastest or not. Well they have a big ship out front, John. Whether they're the fastest or not, we don't want to, but they always find a benchmark for them. But the reality is that people do care about benchmarks and we want to jump into that on the industry standard benchmarks around big data. What are you guys reporting with Cisco? What's some of the numbers you have? So if you look at the benchmark landscape, we have mature benchmarks for system performance from the spec organization and the database performance like a TPCC, TPCH from the Transaction Processing Performance Council. I mean, benchmark is pretty important for the vendors and the customers, and also for engineering organization. I want to talk about two things. One is, if you look at UCF, right? Last five years, we have done extremely well in the marketplace. So one of the things that we are able to demonstrate in the industry is the leadership of Cisco UCS with the 95 plus industry standard benchmark parts. I mean, that is on the system performance side and database performance side. So now, everybody talks about big data. It is becoming a pretty major part of enterprise IT ecosystem across all the major verticals. When you talk about Hadoop and noise quality, it's pretty exciting from a technology innovation perspective. But several of our customers are challenged with how do you size your infrastructure for your application or a new benchmark? What is the right configuration terms of performance, price, performance, energy efficiency? So the TPC has recently announced industry standard benchmark for benchmarking big data systems. I would say this is the first industry standard benchmark for benchmarking big data systems. With this, you'll be able to compare performance, price performance, and energy efficiency of various platforms in a veneral, neutral way. There is no publications yet. I mean, we expect a few publications coming up this year. So how about the interaction with Oracle? Because obviously, you guys have a lot of benchmarks certainly on the Cisco side, different environment. But specifically for Oracle, what were some of the considerations you guys have found that customer scenarios would be most likely the use case for? I mean, the industry benchmarks are great because it's good proxy. But how does that translate to Oracle interactions with the customers you see? Yeah, if you look at today's enterprise application ecosystem, I would put the applications in three buckets. One is the traditional kind of transaction processing and enterprise data warehousing. Okay, UCS, V-Cities and CCDs are ideal platform for those. So we have demonstrated that using several benchmarks, you know, the TPCC benchmark, TPCX benchmark, and two E-Business benchmark that we published this week claiming number one record. So that is on the traditional application space. Then you have this emerging big data space, you know, where Hadoop and NoSQL distributions play a very important role. From a UCS perspective, you know, we provide the best platform for the enterprise application IT where you have traditional applications and emerging big data applications co-exist and complement each other.  So we have announced our new generation platform for, you know, internet of things, internet of things gateways and, you know, processing at the edge with our UCS mini platform. It's basically a 6RU form factor blade chassis in which you have a compute connectivity and storage all in. And, you know, it's primarily for, as I said, internet gateways and branch offices, but it can be managed centrally with the UCS manager. I think the thing with those three, two of them are relatively new. Right, they'll play data space as well as the internet of things space. The other thing we hear a lot about is really the data center transformation driven by power. And a lot of times power is the biggest gate to data center expansion at a particular location. So talk about how the power landscape is really changing in the evolution of the data center and how benchmarking is impacting that. And then the other thing we hear too now is about different densities within the data center based on the workload. How does that factor in?  If you look at our, you know, UCS portfolio, we have a significantly improved on energy efficiency. Okay, if you look at our previous platform versus the current platform, one of the consideration is, you know, 30 to 40% performance improvement at the same power footprint. That has been, you know, something that we have made sure that our new platforms deliver. And on a benchmarking side, there are several industries under the benchmark for power. One is SPECT Power, very popular benchmark. And, you know, look at the TPC benchmarks, energy efficiencies, one of the components that, you know, vendors can report part of their benchmark publications. So talk about the show here. What's your take of Oracle 14? I mean, more buzz this year. I mean, every year it kind of has its own vibe. Four years ago, it was like, dead, quiet, it was like, what's Oracle gonna do? And then all of a sudden, within a year, they just changed the game. A lot more engineered systems. The Sun Acquisition came into play. These have a sort of more geeky hardware piece. A lot more developers.  But let me look at this in a two different perspective. One is on the application perspective. So a lot of applications are moving on the cloud. Oracle made some announcements last night about their commitment to cloud. I think this is very much in line with, you know, the announcement we have made like six months ago about our private cloud strategy, public cloud strategy and inter-cloud strategy. So where you can run your application in your data center, in your public cloud, or like a private cloud in a comprehensive way you can manage your application. You can move up applications from your private cloud to public cloud or public cloud to private cloud. And, you know, I need to understand a bit more on the Oracle announcement. One of the things that are interesting, you know, came to me is the ability to run, like, Oracle Database in a multi-tenant environment. You know, I think that would be the most exciting thing from a software perspective, Oracle perspective in this announcement. And I also see like a lot of companies, you know, on the storage space made significant improvements on performance, especially with the flash storage. So we have done the same thing with our own flash solution as well as, you know, flash solution in partnership with our storage vendors. So what are some of the next big hurdles to cross to increase those benchmarks inside the data center? What are the stuff when you're looking down the road? You're thinking a few steps ahead of most people in the game. You know, on a benchmarking perspective, yes. I mean, we want to stay ahead of our competition so that we can deliver the best to our customers, you know, as soon as, you know, we can. You know, the two things. One is on the compute density, okay? So we have the capability to take a full advantage of the internal process of family. You know, if you look at the process of performance, it has been going in line with the workflow. So to complement that, so we have increased our memory footprint on our server platforms. If you look at our B460 and C460, these two systems are very popular machines in the database space, S54 Oracle. We support up to six terabyte of main memory with the capability to support, you know, 15 gigabytes per second IU bandwidth. This is going to be, you know, kind of the next generation machines for not only for our customer deployment, but to demonstrate, you know, the performance, scalability, and the price performance given to the support of other database management systems. So you touched on it. Talk a little bit about flash and the impact of flash that you guys are seeing as it propagates further and further inside the data center beyond just the super high value, low latency application.  The advantage that comes from two things. One is performance, okay? If you look at the IOs, IOs per second, you know, capability, the flash technology can deliver 1,000 to 10,000 times more IOs per second than spinning media. And on the energy efficiency side, it is one-tenth or like one-twentieth. So, I mean, if you look at five years from now, I would say 75% of all the, you know, transactional and real-time database will be running on flash.  I think the spinning media is going to be more effective in terms of dollar per terabyte. But overall, flash is going to be the future of storage. So Larry was talking on this keynote about big data, and he basically throws it out there like, it's included. It's included. You can't ignore it. You can't ignore it. It was just a direct quote, which I was so, like, happy, but also rolling and laughter because, of course, it's included. Big data is everywhere now. It's part of the platform and everything. But Oracle tries to compartmentalize it like, oh, that's the solution. How do you guys look at how you guys are building out the big data piece of it? Because big data is a big part of the analytics involved in software for the networking stuff, but also the Anthony of the data. So how do you sort out that big data piece relative to the Oracle ecosystem? Sure. I mean, as I mentioned before, Oracle is primarily solving your traditional transaction or data management problem. One of the innovations that Oracle has done is ability to query your data set, outside Oracle database, residing on Hadoop. So treating your data on Hadoop as part of your database table. Okay, so not only you have access to, you know, the data inside your database, but also having access to the data that is residing on Oracle database or other logical distributions. It's very powerful. And the exolytics, how do you think that's going to fit into the whole product issues around customer choice? Sure, yeah. I mean, exolytics is a high-performance analytic solution for in-memory processing. As I mentioned before, large memory is one of the areas that we are focusing on infrastructure perspective. You know, our B460 and C460 supports up to six terabytes of memory. And the new generation of C220 and C240 and B200 platforms, two socket systems powered by Intel E2600 CPUs, we support up to 1.2 terabytes of memory. So in infrastructure perspective, we are fully in line with taking full advantage of like technologies coming from Oracle. So you recently joined as chairman of the Big Data Benchmarks Standards Committee. Congratulations. Thank you. And also being at Cisco, you're covering the data center. I want to take the perspective of looking at why benchmarks exist to share data with potential performance issues and environments that most CIOs or enterprises have. So in your opinion, in your expert opinion, what do you think the biggest driver is in the data center right now for folks out there who are looking at it and realizing that they've got to move to the cloud? They realize there's some transformation going on. In your opinion, what are the big critical things for the transformation, the data center to the cloud? I think the main challenge is your ability to move data back and forth, okay? While meeting all the security, privacy and compliance requirements, there are a lot of things happening in the industry to solve that problem. And one of the major initiatives is our InterCloud also. The ability to provide end-to-end isolation for your application when you send data or application between your data center to another public or private server. So the question I always get, and I'll ask you because you're the expert chairman on the benchmarking committee is, how do you make a benchmark that satisfies all the different audiences and use cases? Because it's hard. You can't always get every use case, but you try to figure out a benchmark that's representative of what the environments do. So how do you guys wrestle with that? So if you look at the industry standard committees like SPEC or TPC, so pretty much all the major vendors are represented in the committee. So all the decisions are taken through a democratic process, okay? The recent... A democratic process by the vendors. Sorry. By the vendors who want the benchmarks to be in their favor. So I mean... Or not, is that true? Of course, I mean like if you're representing yourself in the industry standard in Socia, you wear two hats. One is representing your company. Yeah, of course. The second hat is, you know, your industry standard hat so that when you develop a benchmark, it is vendor-neutral so that your end user customer can compare different architectures, different topologies. So that is the end goal. Yeah, yeah. And you know, what we have seen historically is that the industry standard benchmarks enable healthy competition. Yeah. End conversations too. Yeah, absolutely. Yeah, so... That debate. Debates too, yeah. Debates healthy. At the end of the day, our customers are going to get the most benefit because when there is competition, the performance will go up, price performance will go down. So benefiting the customers. Yeah, yeah. But the key is getting some signal out there that says, okay, here's how generally things look with data to give some navigation to... Yeah, and another thing is, you know, the benchmarks define a level playing field. Okay, so that, you know, architecture X can be compared with architecture Y. Product A can be compared with product B in a vendor-neutral manner. So, I mean, that's the key. That's the key. So I'm going to shift gears a little bit. I don't know if they let you out of the lab and go out to talk to customers, but I'd love to hear your perspective on some things that you've seen recently where people are putting all this power to work in ways that they couldn't do it before. Do you have any good stories from the field?  Oh, good. You know, let me give you a good example. Okay, I have been involved in a research paper. I have two interns working on it, looking at the big data in the health care sector. So one of the amazing things was, you know, HGP, the Human Genome Project, that is one thing that has output form more slow. If you look at the... The scale of that thing is huge, right? Yes. Give us some numbers that kind of map out the scale of what the... If you look at the 15 years ago, the cost for genome sequencing or a fraction of a DNA, I think the metric that they use is Sinti Morgan. To process that would foster something like $10,000 in 2001. Now it is in less than 10 cents. Okay? So if you look at the product against more slow, it is significantly beating more slow. I mean, that is one thing. I mean, the great potential of big data and the genomics combined in the health care space, especially for personalized medicine, you know, that can significantly change the health care industry. So we can only live longer, 1,500 years more. Yeah? Well, I don't know. Two years more. Okay. I'll get you the final word in this segment. Thanks for coming on the queue. Share it to folks out there if they were to ask you, what's the big deal this year at Oracle? What's the big technical focus at the show? I think what I've heard so far, the main focus is a database in cloud, a database as a service, you know, in a multi-tenant environment with, you know, all the necessary isolation and, you know, security and privacy-challenging address. Okay, security. Okay, we are here live in the Cube. This is the Cisco booth. This is the Cube. We are broadcasting for Two Straight Days. I'm John Furrier with Jeff Frick. We'll be right back with our next guest after this short break.


We hold these truths to be self-evident that all men are created equal, that they are endowed by their Creator with certain unalienable rights, that among these are life, liberty, and the pursuit of happiness. These words from the Declaration of Independence are familiar to many of us, and yet it took 143 years for women to get the right to vote and 189 years for Black people to get the right to vote. And still today, life, liberty, and the pursuit of happiness are still only words for many people. Here in Boston, life expectancy varies by 30 years, depending on where you live. In Roxbury, with many poor and Black people, life expectancy is 59 years. In the back bay, wealthy and mostly white life expectancy is 91 years. It's tough to have liberty when you are in prison. The United States incarcerates 716 people for every 100,000 people. Our rate of incarceration is more than five times higher than most countries in the world. Millions of people in our country don't have health care, a decent job, good education, a home they can afford, and that makes it pretty hard to pursue happiness.  People today who are making the words of the Declaration of Independence come true. Hi, I'm Michael Jacoby Brown, your host of We Hold These Truths. And today we're very lucky to have with us Lou Paddy, owner and operator of Gray Paddy Auto. Gray Paddy Auto has been in Arlington for almost 50 years. Lou's been working there. And when we think about life, liberty, and the pursuit of happiness, liberty often involves transportation. And I don't know about you or what planet you live on, but to get around Boston, unless you got all day, and don't worry about standing outside at a freezing bus stop for a half an hour, you need a car to have any kind of liberty. I often work in different parts of Boston, and I drive there. And one of the reasons I drive there is because Lou Paddy, for the last more than 20 years, has made sure my cars are able to get up and go every morning. And Lou's been working on cars for over 50 years. So Lou's really an important part of anyone who cares about liberty, because getting around in this city, in this country, in this state really often requires a car. So Lou, welcome. It's nice to have you here. And thanks for being here. I wonder if you can tell us a little bit about where you were born and how you ended up coming to Arlington. Well, nice to be here, Michael. Thank you very much. Well, I came here as an immigrant. I immigrated from Belgium in 1961. And after a short stay in Somerville, Mass, we moved to East Arlington in about 1966. Wow. And how did you get involved in the auto repair business? Tell us a little bit about it. Just while growing up, I was involved with people that were mechanics, mechanics, crackerjack mechanics. And I was self-taught. A lot of things that I did started out with small engines. Until again, I ran into some mechanics that were kind enough to teach me a lot of stuff. Where was that? Well, actually, I ran into a mechanic working at Mahoney's Rocky Lech Farm when I was old enough to get my working papers. I think back in the day, you could get your working papers at 14 or 15 years old. And immediately, this mechanic recognized the fact that I could be really helpful around the machines that they owned at the farm. You had an aptitude for that. I just kind of had a knack for it. Yeah, no, it's great that some people are good at that and some people not necessarily. It was something that came naturally to me. Yeah. And so tell us a little bit about starting Gray Patty. I mean, many of us know Steve Gray, unfortunately. Unfortunately passed away. Six years ago. I started at the gas station in 1974. Really? Sweeping floors and pumping gas. Which gas station was this? This was a Sonoco gas station that used to be across from Arlington High School. Oh, right. No longer there. Right. So I started there and Steve recognized the fact that I was worth a little more than just sweeping floors. And at the time, I had graduated high school in 1974. And after I took some automotive classes at high school, I enrolled in a program at Benjamin Franklin Institute of Boston in 1974 and graduated in 1976. At the same time, working for Steve Gray. And not only pumping gas, but working on automobiles under the supervision of another mechanic that I met in my life that helped me out a lot. Who was that? His name was John Anderson. John Anderson? Yes. It's good to say their names. Yeah. Because that's how we learned from people. And he was really good to me. He really helped me out a lot. He taught me a lot. Now is he still around? He is retired. But he's still around. He's still around. Yeah. Oh, that's great. And he taught you a lot. Yes. And tell us a little bit about starting Gray Patty Auto. I know that was almost 50 years ago now. I started in 1974 and in 1980, after working for Steve for six years, obviously, he offered me a share in the business, a half share in the business. Really? Wow. And I accept it, of course. And he needed somebody that he could trust, that he thought he could trust to take care of business so that he could take some time off and he could enjoy life a little more than just working. Yeah. Gas station businesses, the hours are just endless. What's it like? It's tough. What do you mean? With the regulations and back then, the paperwork involved in dispensing gasoline, purchasing, storing and dispensing gasoline. It was just, it was 24 hours a day. Really? What do you have to do? Because I think a lot of people don't know that. There was a lot of state and federal regulations that came into play in the 70s that made it very difficult to handle the product. Really? And the oil company was tough on their dealers. Earlier in the 60s, let's say, maybe the early 70s, the oil companies worked for the dealers. Really? But in the 80s, late 80s, the 90s, the dealers started working for the oil companies. And the oil companies became very strict on their pricing structures. They wanted their product to sell more than the product up the street, than the gasoline station up the street. And it was a very competitive market. Really? It was tough. Let's talk about cars themselves. You've been doing this since the 60s, right? Well, in the 70s, I was working on 60s cars, maybe even some late 50s cars that people still had around. So yeah, the 50s and 60s technology was at our doorstep when I was first starting in the business. Right. So how have cars, and now you've been repairing cars too, keeping people's liberty going for a long time. How have cars changed over all those years? The biggest changes have been in manufacturing, of course, a lot of lighter materials, a lot of plastics. Big changes came to the emissions and safety standards that this country was developing to try and create a product that would be less intrusive to our environment. In the middle 70s, the EPA, I believe, was formed and that was a big hit for the auto manufacturers because it was crunch time. It was time to clean up the tailpipe. So the automobile manufacturing really had a big job on their hands. The 60s were very dirty. The 50s were even dirtier, the automobiles. And if it wasn't for the EPA implementing these rules and regulations on an escalating basis every year, the manufacturers had to provide automobiles within their whole fleet that they offered to the public a certain number, a certain miles per gallon number. It was something that they had called cafe regulations. If it wasn't for the introduction of all these rules and regulations, although as strict as they were, it would be a lot dirtier around here. The air would be unbreathable. I think that it was a good measure by the U.S. government to implement some kind of strategy to clean up the air. A lot of people now obviously are concerned about global warming because most cars, except for electric cars, produce emissions that create the carbon in the atmosphere, which is causing all these storms and fires and all over the place. And yet most people to get around still need a car to get to work.  Sometimes she can ride her bike and take the bus or the subway, but sometimes it's just not working. And in Boston's weather, a lot of times it's pretty uncomfortable to stand outside when it's 20 degrees waiting on Mass Ave for the bus, for the bus could take, I mean, I've been waiting out there sometimes when I do take it, 20, 25 minutes. So cars, unless something really changes, are going to be with us. I agree. So how do you see the future of cars in personal transportation? You're someone that's been, you know, so to speak, on the front lines for decades. The future of cars. Well, I think the future is pretty evident that the manufacturers involved around the world are no longer going to produce gasoline-powered automobiles or fossil fuel powered automobiles. A lot of the manufacturers have stated that. And they're going to go to the next best thing, which is, I think the next subject was the electric car thing. Right. We have some of that, but not a lot. Not a lot. Not a lot. I think that the future of the automobile, I still think it's going to have four wheels. When a wheel falls off, I've had that happen. Three, that happens. Yeah, that's kind of dangerous. I had one fall off at about 60 miles an hour once. It was not a happy experience. Yeah, really. I could live without it, probably. I see, I recently read an article about, again, a Wolverine company that builds flying automobiles. I don't know. I'm not so hip on that, but flying automobiles have been produced for decades. They just never got off the ground, so to speak. Really? Sometimes I'd like to have one when I'm stuck in a traffic jam. What are they?  I just don't get it. You've got to take off somewhere and you've got to land somewhere. Do they stop at red lights? When they're not flying, yes. I think they're called helicopters, aren't they? No, no. Actually, with wings and the amount of passengers depends on how much power is built into it. It's pretty strange. I don't think that's going to happen. I could just sit there crashing into each other. If it's got an engine, it's going to be a problem. What about the electric cars? What's it like, 2% of cars in these states? According to AAA, John Paul, I believe is the gentleman's name, the Alican advocate recently had an article. John Paul spoke at the Alican Men's Club, a retired men's club of violating, excuse me. He stated the fact that right now this country is utilizing approximately 2% of the vehicles in this country now are electric. If there's a sudden rise in that percentage of vehicles in this country, it's going to present a problem with the electrical grid. The available electricity is not there. Now, this morning I heard that part of the president's push on the structure, the whole structure deal, they want to fix all the bridges, they want to fix everything. Part of it was installing charge stations all over the country, and the government is going to fund that, subsidize it possibly. But I think that should have been taken care of before the electric car really started becoming more popular. Personally, I think that the hybrid product is still makes more sense. Because you've always got a filling station around. A filling station to me is whether you're filling it up with diesel fuel or gasoline or electricity, how come all the gas stations in Highlington or in any town, city, how come there are no charge stations in all of these areas? It is a filling station, right? Good question. Yeah. Ryan, what do you think it would take to change that? I mean, you know cars themselves, are the electric cars, what are they, I don't know if you repair them or if they need repair? We do not repair them. And we limited on the hybrid car repair simply because I haven't taken advantage of the education available. Because I think it's going to take a few years to learn how to repair the cars properly, the electric part of the hybrid cars. And I'm not, you know, I'm at the end of my after 50 years or more. After 50 years, I think I'm going to stop trying to learn about new stuff. And that may be the wrong approach. But I'm more comfortable trying to sustain the energy and the repairability that I can handle now at this stage of the game. But I still think that hybrid is the way to go. Electric cars, electric cars are going to put people like me out of business. Oh, really? Personally. Now, the maintenance on the electric cars is pretty much, I'm not going to say nonexistent. But it's a much cleaner operating vehicle. And that's part of the reason it's eventually going to become more popular. But it's less moving parts. We don't have all kinds of transmissions and transfer cases and engines to all orchestrate properly to make this vehicle go down the road. And not only here on Mass Ave, but on the highway at 60, 70, 80 miles per hour safely. The electric car is just incredible. Really? The electric car just at the snap of a finger. No noise. But it must have some moving parts. I mean, the wheels got to move. It's got moving parts. But I'd say less than half as much as a conventional automobile, probably 25%. So do you think it'll be, what it'll be like in terms of repair and longevity?  Long time. Right, thank you, Lou. That's got 172,000 plus miles on it. Fatted for, I don't know, 10 years?  Does the electric part of it actually create more longevity as far as, as far as how long the vehicle lives? I don't know. Apparently, it does a pretty good job, because there's a lot of Priuses out there. All the Priuses that are still going, and they're still in pretty decent shape. Right. But they're not totally electric. They're hybrid. They're not totally electric. They still have a small gasoline engine. Yeah. But obviously, it's not overworked. I couldn't tell you, I couldn't tell you, if I've ever seen a Prius, that I personally have had to replace an engine. Really? Just, if properly maintained, I think that car will go 300,000 miles. If it doesn't rust away, you know, everything as far as if it doesn't get crashed, if it doesn't rust away, there's a handful of things that are going to go wrong, but it's totally acceptable. Right. Well, what would it take to have, in terms of, you know, improving the environment, and if electric cars are the future, you said a lot of manufacturers are going to be only making electric cars. Only making, I don't know if strictly, for example, Volvo announced maybe last year that they were, in five years or four years, they were not going to sell internal combustion automobile. But, well, okay, so that answers my question. Internal combustion means that it's not going to be a hybrid. Right. It's not going to be a hybrid in either way. It's going to be totally electric. Right. So it's got to be a plug-in, or it's got to have some kind of means of recharge. But what would it take to have more electric cars? You said there aren't enough charging stations. The infrastructure is not there. I mean, what would specifically do you think it would take? It would take electrical generation, bigger power plants, larger power plants, more solar, you know, solar to provide electricity to power the automobiles. I'm not an electrical engineer by any means. I would think that the charge thing would have already been implemented. It's kind of like taking the carriage before the horse. Right. We've got the product. The product is here. But I think if you talk to some people and they say, this is not my travel car, if I want to travel, I'm going to take the Prius. Right. You know, I'm not going to take the Tesla. Really? Okay. You know, I'm going to keep my Tesla, but it's going to stay inside of 128. Really? You know, and I'll be fine with that because I'll never have to worry about recharging it. But if I'm going to drive out to Ohio. Yeah, although I told you a friend of mine drove from Minneapolis to here in his Tesla. He said it was all... Well, if you go to Tesla, you need to be... You need to have one of those powerful phones that will tell you where all the charge stations are. Yeah, I guess that's what he did. And if there's other people at the charge station, you're going to be there for a while. I see. Right. It must take a while. It doesn't charge right away. It's not like filling my car up with gas that takes five minutes. Right. Not at all. Not at all. Might as well watch a movie. Well, what would you say to...  What would you say to young people thinking of getting into repairing cars? It's a...  It's kind of a tough question. What would you say to them? What I see on my information databases that I have at the garage is, you know, if I look up a Prius and I need some specifications, I just jump in and I enter all my information and it comes back and then I pick out what I need. Now, if I go to Tesla and my information database is updated every month... Really? Wow. There's no information on Tesla. Really? I can't even tell you how these automobiles get repaired. I'd like to someday go to a Tesla dealer and see what the mechanics are doing. I don't even think they have dealers. I think you buy them all online. So, you know, that being said, you know, what are your recommendations to a young kid who wants to get into the automobile industry, stick it out and just work on automobiles until everything's electric? How long is that going to be? I think it's going to be a while. I think you probably... Another 25 years? I think after 25 years, you're not going to see the car in garage anymore. Really? I could be totally wrong, but the way I see it's going, I can't afford to buy the computer networks and the software to work on these automobiles today. I would have to specialize on, for example, VW and buy all the equipment just to work on VW to repair the product accurately, or BMW. It's the same thing. The software and the hardware from either manufacturing basis, I'm not going to be able to use it on another manufacturer's product. And it's the same thing with Chevrolet, and it's the same thing with whatever brand is being produced at this time. So it's very expensive to buy all the data? It's very expensive to be able to... Okay, so just a quick example when we were at the gas station, we got along with the Buick dealership down the street. I bought a lot of parts for GMs through the Buick dealership, which is no longer there, but the way, which is the CVS now. And they worked on Buicks. If I had a problem with a Buick and I couldn't figure it out, I'd go down to see my buddy and say, hey, what do I do with this? And he'd be like, well, look in this area and and he'd give me some pointers. But if an Asian car ever came into their shop, you know, everybody was just, well, what do we do with this? Send it to great baddies. Let me... I'm going to go up and see Lou and Steve and see what they can tell me about this. When the Asian automobiles came to this country, immediately they were in our shop getting maintained. More than maintenance, they were getting repaired. They were no better than the American cars in any way until...  Until the late 80s, the early 90s. But back to the fact that somebody... I see that Minutemen Tech has just reorganized. Right. And they've even changed the name. Not sure about that. Minutemen Regional. Really? They changed the name, but they're still keeping the automotive technology program. And I say that because I think my daughter had showed me what the tuition base was now. There is a tuition base now at Minutemen Tech. I don't know what I would recommend as far as somebody that wanted to get into automotive technology nowadays. Like I said, I think it's changing. It's ever changing. You have to be more computer literate than us dinosaurs. Well, you're no dinosaur. Well, I want to thank you, Lou. Our time's about up. But I want to thank you. You've been taking care not only of my car, but probably half the people in Arlington's cars. And when we talk about keeping the truths of life, liberty, and pursuit of happiness, I know for one. And I bet for many of you, being able to have an automobile that's reliable, you want to go to Lou Patty, you know, Gray Patty Auto on Mass Ave in Arlington to have it fixed. And part of liberty is to be able to get around. And I don't know what the future of personal transportation or public transportation is going to be, but I think I agree with Lou Patty that cars are going to be with us for a long time. So thanks for tuning in. My name is Michael Jacoby Brown. I'm your host of We Hold These Truths. We were really honored to have Lou Patty of Gray Patty Auto, Arlington institution with us today. And we hope you'll tune in next time. Okay. Thank you very much.


Hello everyone, let's continue with the remaining tutorial of Arabidopsis whole transcriptome analysis You can always quickly access the trading material from here first scroll down and click on the transcriptomics topic and then Go to the bottom of the page and you can find our hands on Here So far you have analyzed the micro RNA sequencing data as Described here in this upper part of the figure So you first started with the micro RNA reads and then did some quality control using fast QC and multi QC and Then trim the low quality reads and creep for the adapters using trim galore And then you quantified the micro RNAs using Miradip tool tool and then you continue with desec 2 for differential gene expression analysis and Then you selected the up-regulated micro RNAs out of them And then now the this the second part of the tutorial deals with the mRNA data analysis So you start with the mRNA reads here and then do similar quality assessment as before and then this time we use Salmon for quantification and Use desec 2 for differential gene expression analysis now we will extract the downregulated mRNAs and finally we will use Micro RNA target prediction program called target finder to find put it to targets of Upregulated micro RNAs in downregulated mRNAs That's the overview of the workflow now. Let's jump into the hands on If you haven't already, please import these four fast Q files that correspond to the mRNA sequencing data into your history You can either follow these instructions or you can watch the previous tutorial If you're using the history from the previous tutorial, you should already have these fast Q files in your history I'm continuing my analysis in the history from the previous tutorial So I should have them in my history already. There they are First things first, let's add some tags to our data sets Similar to what we have done with the micro RNA data set collections For this collection we give tags control and mRNA For treated samples we give tags PR and mRNA Now let's go into the training material and directly jump into the mRNA data analysis First we do some quality control using first QC tool So just click on this and Then we now select the collections from control mRNA data sets first and And then click on execute while it is running we can Rename the outputs, so just copy this text from here and rename the first QC raw data set collection. We go back again and copy the Text for web page and rename the collection of the web page Now first QC jobs are complete now Repeat the same steps for the PR treated samples now select the first Q file collection on PR treated mRNA and then Click on execute now go back and then we copy the text to rename the files again So but this time we should actually change the control to PR treated now we have to Copy this and then Changed other collection name To the web page Now we are done with both the first QC jobs And now we merge the collections of the first QC outputs we select the Raw data collection from the first round and raw data collection from the second round and then click on execute Now we can use multi QC to summarize this fast QC results in one single report So select fast QC Here and now choose the data collections and then click the click on the merged collection We should also rename the title of the report to mRNA quality check so copy this and then press here Click on the execute the multi QC reports are ready now. Let's visualize them Here we see some general statistics Okay, so if it's not loading just click again. Yeah, here we see some general statistics on JC content millions how many millions of reads duplicates and If you scroll down, we see the number of reads the absolute number of reads and Sequence quality histograms looks fine And the Persequence quality scores also look fine And the GC content also fine And they are also not many duplicates But it seems there are some adapters. So it's Illumina Universal adapter present in the reads So they're present in all the samples but Not too many So it's less than 2% of the sequences contain the adapters and everything else is fine. Let's Go back Try to answer these questions while you're doing your hands on if you cannot you can always use a solution from here Although there are adapters in all samples. We are not going to clip them the main reason for this is Usage of the Solomon to quantification Solomon does simultaneous mapping and quantification That's not to any base-to-base alignment, but by mapping and quantification to infuse Which reads are originated from which transcripts? As the KMS that corresponding to the adapters in the reads are not part of the Reference transcript home. They are not counted Hence, it's not mandatory to Clip the adapters when using some let's now perform mRNA quantification by Solomon one tool choose the reference transcriptor from the history And then choose our transcript on first the file leave the cameras as they are And now we select our fast queue file starts a collection So we start with control mRNA collection And then we See yes to validate mappings Then then we have to select the File contains mapping transcript genes that is our gdf file and then That's it I guess we have all the options set now We go back and click execute while Solomon is running. Let's rename the outputs first copy this and Rename the corresponding Collection then go back and rename the other collection to Add gene We are done Now we have to repeat all the steps again For the we are treated samples. So click on someone contact in Select the transcript on first the file Now this time we select Collection that belong to the BR treated mRNA samples Set the valid mappings to yes Select the gdf file Then click on the execute button. We have to rename the files again. So copy the names again paste them, but this time we have to Replace control with BR treated and we also have to do the same for the gene quantification files, so replace control with BR treated and Add gene So the song run will take Some time to finish Solomon produces two output files first one is quantification and the second one is gene quantification file Quantification file summarizes the quantification at transcript level and the gene quantification file Summarize the quantification at gene level if you look at one of the files It Contains these five columns So the first one is the name name of the transcript or name of the gene the second one is the length or sequence length of the transcript and The third one is the effective length that is calculated by Solomon So it is kind of correct a length which takes sequence specific bios gc bios and fragment length distribution into account And then Solomon also Calculates Anomalyzed Expression levels in TPMs, which is transfers transcripts for million TPM calculation is based on the effective lens and I'll solve one also Outputs the absolute number of reads but transcript in the last column now, let's go back to the Tutorial So far we have done with the Quantification of the mRNAs now we are Ready to perform differential Gene expression analysis using these two So first we need to give a factor name so as we want to know the effects of the Resinolide We Give it that name and the factor and the factor level is What we are interested in first? So the present right is the factor level the first factor level and then we select the samples that are treated So go to the collections and select The gene quantification files Right control for the other factor level and now we select the control Collection then we Tell the second to that the values Coming from Salmon, and we also give Annotation file so that maps Transcripts to genes and then we click on execute Our DC2 is running. Let's rename the outputs So click on the edit attributes and change the name to desect plots mRNA Save it and then change the results file to De-sector results mRNA Save it Let's inspect the de-sector plots The first plot you see here is PCA plot As you can see the samples from the same experimental conditions are Clustered together signifying High similarity Between the biological replicates you can see the same trend in the heat maps too As you can see in red even with this sub-sample data set we found some differentially expressed mRNAs Let's Go back to the Tutorial So all these plots all the results of de-sector are actually generated from sub-sample data. So Before continuing further with the filtering we get the results from the complete data sets. So copy this link and then Upload the data So click on the paste fetch and then click start We should rename this file to mRNA de-sector results table save it and then First we need to add all the tags that are related to the mRNA data analysis. So we add First our mRNA Br and control so mRNA Br and control should have been renamed already. Okay, let's save it again. Let's go back to the tutorial So now what we do is we filter for Differentially expressed genes up regularly to microRNAs and downregulated. Oh, sorry.  So now we have to select the this filtered list of genes. So Then C3 Column that contains the four changes with greater than one that is Absolute two-fold change and then minus one for downregulated. So now we still select the List of differential express genes now rename them to Differentially expressed mRNAs first And this one has Upregulated mRNAs and this one has downregulated mRNAs Save it. Now you can answer try to answer these questions by yourself So first we can look at how many genes are differentially expressed. So this is like 4176 genes That are significantly differentially expressed and there are 778 upregulated and 328 downregulated genes. To answer this third question. What is the most significantly? Upregulated gene and what is its function? You have to copy this gene ID You can search for it. You probably find a result that is in their database So it encodes for P450 enzyme that catalyzed the production reaction production of Resinolide. So it seems this is a very relevant gene for our analysis And it's no surprise that this is differentially the most differentially expressed mRNAs. Let's go back to Tutorials now we have all downregulated microRNAs and Upregulated mRNAs Now it's time to find targets of the microRNAs that are in the downregulated mRNAs So to do this we first need to extract the sequences of the microRNAs and mRNAs We have to first extract the IDs of the Genes and then extract the sequences. So we start with the microRNAs first use the cut tool to cut out the first column which contains the gene ID from the upregulated microRNAs first So we cut the first column which is C1 and then Click on execute this as upregulated microRNA IDs The job has started and finished so we can rename it Save it If we inspect the file they are on total 16 Mature microRNA is a microRNA star sequences IDs So now we have to filter for sequences using filter faster tool So once we select the mature microRNA sequences first the file and second time we select the star microRNA sequences So we start with the mature microRNA sequences first the file So this file contains All the mature microRNAs and the first format you have the ID and then in the next line we have the sequence So select the mature microRNA first the file and then for filtering we Provide the list of IDs and we say we have to select upregulated microRNAs and Then match IDs by default, which is simply a greater than symbol followed by ID then we click on Execute so there are nine sequences out of 16 which are mature microRNAs And the remaining seven are the micro are from microRNA star sequences so we Repeat the filtering step again But this time we select first the file from star sequences So select star microRNA sequences first the file Keep the list of IDs as upregulated MicroRNAs and then we can execute this file should contain Seven sequences Once we got both the files we concatenate them Using cat to see here seven sequences that belong to MicroRNA star sequences and then nine mature microRNA sequences So we concatenate them So we first select the results of first filtering and then select the result of second filtering And then click execute now we rename the output to Upregulated microRNA sequences here say Now let's inspect the final ones so we have Total 16 MicroRNA sequences and now we go back and continue to Obtaining the downregulated MRNs. So now we do a similar step as before to extract the IDs so this step he rerun this step We select the first column, but this time we select downregulated MRNs So now we are selecting the first column that is the IDs of the genes from the differentially expressed and downregulated MRNs First rename it to downregulated mRNA IDs Here if you inspect it. So we have like 328 IDs So 328 upregulated MRNs total and This time it's not so straightforward. So we have all the sequences in the transcriptome first file Transcript term first the file header is a little bit different than the microRNA first the files we've seen before but this has a bit Bit complicated header. So it has a transcript ID first and then we have Gene ID What we have to do now is we have to select this gene ID from here But it's not at the beginning of the line. So we cannot directly use it The default option to extract the sequences we do trick to extract the sequences here You again use filter faster tool for extracting the Sequences out of transcriptome first the file. So but this time we have to write a kind of tricky regular expression To extract them. We'll see how to do it. So first We select the transcriptome first the file and then a list of IDs And then the IDs are downregulated mRNA IDs And then now we as we can see the ID is not at the beginning of the Faster header, but somewhere in the middle. So we to match it properly We have to write so-called regular expression So we have to match This which is in the middle of the hell So if you look at the gene ID start with the Gene is equal to and there's ID of the chain And the ID always contain ET at the beginning and then followed by seven Alphanumeric characters So this is what we are going to use Regular expression is gene is equal to a ET dot Seven so we copy this so what we are doing here is we Want to select Everything that is in this brackets. So Now we are matching everything that starts with a T and then has Anything the dot corresponds to anything any character seven times 80 followed by seven characters This is why we are going to match so Gene is equal to 80 followed by any seven characters. Now we rename this status to downregulated mRNA sequences now we have already Upregulated to microRNA sequences and downregulated mRNA sequences so we can Continue with micro on a target prediction with target finder so here we select upregulated micro RNA sequences and here the downregulated mRNA target sequences we So in this case we are a bit lenient and then we say predictions go off to set to 5.0 then we select the tab delimited format which is More condensed format than the default output format. We'll see what we get It's ready now if you visualize the file that target finder found targets for some micro RNAs but not all so you can see there are No results found for some of the micro RNAs. You can also see some micro RNAs have multiple targets For example, this first one has multiple targets So the first column is a micro RNA ID second column is this second column is the target ID This is the position on the target where the micro RNA is aligned to and That's the strand And the next column is the score So as we put the scorecraft of 5 everything below 5 will be there And then we have the sequences of the micro RNA and the mRNA And the middle is the actual alignment between the micro RNA and the target mRNA sequence So you can click on this each of each of these links to know more about these target genes We propose the following Hypothesis from our results. So if you inhibit any of these genes the Plants can have higher resistance to draft condition as a validation experiment You can acquire the mutant seeds and wild type seeds and then grow them on top to control conditions one With watered and the second one with drought stress And then you analyze the plant weight after 33 days and you'll see if the mutant plants have improved resistance to drought condition So that's all For this tutorial and if you want to analyze more data We provide an optional exercise So if you are done clean with this analysis, you can analyze this later Do it now or on the last day of the workshop where you have more time That's all for now. Please give us your feedback and Thank you for your participation


It's quick tips. We talk every week. We give you something quick. You want to know what every call is worth You want to succeed at phone calling? But a lot of times we don't realize what picking up that phone can actually do for us or can bring us or can we can We can get in our life or achieve just because we did something we didn't enjoy We picked it up something. I love to figure out. What is every phone call worth when you pick up the phone? What's it worth? $20, $20, $20. I bet you pick it up a lot more if you knew that every time you pick it up is worth 20 bucks What is your phone calls worth? Track your dials, track the appointments set, track the percentage of appointments that you close. Maybe you make Maybe you make 10 phone calls and you set two appointments and you sell one. If that one sale is worth $500 Then those 10 phone calls were worth $50 each 50 bucks 50 bucks 50 bucks I guarantee you you would pick it up a lot more start to pick it up Because it's money waiting for you to pick it up


Well, I think inquiry is all about, you know, just providing those avenues where students are becoming engaged and excited about the learning, and they want to find answers to their questions. And the excitement is so rich that they're actually going home and they're talking to their parents about it, and they're talking to their friends, and they're getting others excited as well. That to me is inquiry. And I love the freedom the kids have to go after their own questions. So that's where I see the shift in the two curriculums, the old and the new, is that we don't have to come up with all the right questions to ask them. And we're facilitators in the process, like we're guiding them through it. And it always amazes me too when you observe classrooms where inquiry is prevalent, how students don't even have a realization of time. They're just focused and the bell will ring, and you know, I've even heard stories from some teachers that they don't even want to go out for recess in elementary, and that to me is amazing. And the stories around the supper table are about what we learned at school today. And again, powerful when you have teachers coming up to the principal saying, I don't know what they're doing in social studies, but keep doing it. And the fact that I've also heard some parents say, geez, you know, I've actually had to go on the internet and learn some of this stuff because, you know, my son or daughter keeps, you know, coming home and asking me these questions. And I'm getting interested, so it's leading to inquiry for them as well. So I think it's a great tagline. Inquiry learning is contagious. It is. That's a great tagline, actually. It is. Well, I think it just, inquiry lends itself to this new program. And I know that my work with teachers when I started with implementation, just sitting down with them and looking through the outcomes, there's a lot of fear because there are many, many outcomes. And you know, a lot of teachers were looking at how am I going to, you know, cover all of this. But teachers that embrace the new curriculum and the shift in pedagogy and of who have incorporated inquiry have quickly realized that it's not always about coverage. It's about uncovering the outcomes. And by using the inquiry process, they're amazed at how many outcomes they actually cover by having students go through and do the research and find answers to the questions and being a facilitator in the learning and developing that community of learners atmosphere. For me, it's just simple things to get an inquiry. You know, just using those clips, like going on YouTube if your school doesn't have a block, but there are ways around that. Teacher tubes, another one. Video clips, quotes, just to spark interest. And that's an easy way to get at it, and it can lead to greater projects or inquiries. Because I think sometimes teachers, when they think of inquiry, they think, oh, it's this long drawn out process and I don't have the time. And that's not always true because it can just be something as simple as a question on the board. And a what if question. And ask students to try to find answers or make meaning of that question. I like your reference to all the strongly visual elements. Today's digital kids love the visual. 21st century learners. Right, 21st century learners. So we need those short video clips. We don't need a 35 minute movie. No. Or we can use some cool web 2.0 tools like Animoto and Voice Thread to really engage their interest and get them wondering and inquiring. Absolutely. And for me, the very best practice I think for teachers is they need to be curious. They need to model the curiosity, the inquiring minds themselves and not have all the answers and be willing to learn along with the kids. Kind of the shared learning I think is really powerful. And again, just to touch on your comment about Animoto and Voice Thread. Because again, I know doing sessions sometimes there are teachers in the audience who are a little bit scared because it's a technology and maybe they don't have the background. But I just would like to say that teachers that I know that have tried this, a lot of times they don't have the expertise. And the amazing thing like you mentioned about 21st century learners, the kids know how to do these things. And again, you're just facilitating and there will be students in the room that can help and it's very, very easy to do. So I wouldn't be scared or worried about trying to use these things because the kids will figure it out. And that's a shift, right? But we don't have to have all the expertise in the room. Some of it can come from our kids. And they can shine and lead during different parts of the inquiry process. Absolutely.


Hei, rydw i'n meddwl yw'r 4th ymddangos i'r bobl yn ymddangos i'r Sefydl Cymru, i'r ddod i'r ddysgrifeth yma i ddim yn cysgolio'r ddysgrifeth yma i ddim yn ei ddod i'r ddysgrifeth yma i ddod i'r cyfnodau Cymru. Rydw i'n mynd i'r 1700 ymddangos i'r ddysgrifeth, ac yn gwych yn ymddangos i'r gyfnod ar gyfer y dyfodol, 14 gwahanol amdano yn go功wys iawn a wrth11 mae'r gweithio, y gallwn yn llaw'wch yn blaen i'w chaosol yn ddechrau'r dweud.�던, there's still about 700 of you who haven't done so and we've got a small army of people ringing round and trying to track you down at the moment to encourage you to go on our website, send us your details so that we can get this money out to you as quickly as possible, Thank you to those businesses who wrote to Cambridge Independent to thank us for turning round their own applications in a matter of four days. It's always really nice to get some compliments back. If for a business which can pay it's bills please do so, I was impressed to learn this week that Taylor Wimpee and some of the supermarketsபeiliw fighters are paying their suppliers upfront. It's really important that all of us keep our cash flow going so you can help your own business own business but you can help other people's businesses as well and finally if you're a small business who we might not know about please make contact with us because we have a one-stop shop of information at the council that we want to share with you of all the information and advice that will help you to stay in business and come through this crisis in a good state so please email us at greatercambridge.business at cambridgea.gov.uk so have a nice weekend enjoy the sunshine stay in business stay safe and stay well


Commander Terry Wilkett continuing to fly a very precise course up toward the Mir space station and this view from the Mir space station looking at endeavor as it continues its approach toward the Mir now at a distance of just about 45 feet. And this view of the crew cabin of Endeavour from its overhead windows and these are the overhead windows in the crew cabin of Endeavour as it is now just about 43 feet away from Mir. This view coming from the Mir space station as Endeavour approaches the 30 foot distance from the Mir space station this view of the overhead windows in the crew cabin of Endeavour. Very shortly the crew will begin its station keeping at the 30 foot level before receiving their final go decision to proceed for at the docking with Mir that docking expected just about 11 minutes from now. The docking will take place in darkness as this view also is in orbital night as the two spacecraft now are some 209 nautical miles or 240 statute miles high over the African continent. The crew is reporting that they have sighted the docking target on board Mir and that no course correction is required at this time and the crew is now beginning its final approach to the Mir passing inside the 30 foot mark as it continues a very slow approach for docking with the Mir space station. Endeavour now within 25 feet closing at a rate of about one tenth of a foot per second and contact between Endeavour and the Mir space station confirmed on time at 2.14 pm central time. This view of Astronaut Dave Wolf aboard the Mir space station as Endeavour is now initiating a post-contact thrust maneuver to drive the two rings together to create a hard mate. And again Dave Wolf aboard the Mir space station and now docked to Endeavour along with Flight Engineer Pavel Vinogradov. The crew is now ready to meet the guests. It's an impressive sight actually. It's just amazing. It's been described as a big mosquito or dragonfly and it's right. It's got all kinds of wings on it. It's an incredible sight. Once Commander Anatoly Soloviev is done with his activities opening up the Mir hatch as well as some housekeeping activities at the conclusion of that the shuttle will begin a pressurization equalization prior to the opening of the door from the hatch from the shuttle side allowing the Mir 24 and STS-89 crews to greet each other personally. You have a go for hatch opening. This is mission control Houston. Hatch opening and a first greeting between Mir 24 Commander Anatoly Soloviev and STS-89 Commander Terry Wilkett as Endeavour Mir are passing just to the southeast of Australia. And greetings all around. And an exchange of greetings continuing as the Mir 24 and STS-89 crews meet and greet one another. The crew is now ready to meet the guests. You guys look great. You guys look wonderful. This is really fun. This is really fun. There's a space shuttle down there and there's a real space station that way and we have a place for you. Thanks for coming to get me. Although I could have lived a lot longer psychologically physically but there's a lot of fun and good business to be done on Earth. It's time to go back I think and stand the ball over to Andy. He's very capable and should enjoy this. I'm very happy to welcome the crew of Terry Wilkett that had so magnificently performed the rendezvous and docking and we're very happy to see our friends who have arrived to work together with us on the Mir station. Our friends from Star City. Thank you Anatoly. It is our honor to be here on Space Station Mir. And we thank you for your welcome. We have looked forward to picking up our friend Dave and bringing him back home. And of course we'll be sad for leaving our friend Andy. But we know that another crew will be here shortly to pick him up. And in the meantime I'm sure he is in good hands. We have some gifts that we would like to present to you. Here is some fresh fruit. We have a couple of other things here. We thought we would spur to pick up some chocolate space shuttles as a crew notebook that we use on the shuttle with some shuttle some space Indians for both you and Pavel. Thank you for welcoming us aboard. And last one, a Swiss Army knife with a shuttle image on the side. Most importantly we have brought you this gift. It's confusing for a year and a half now. I'm not sure whether I live in Russia, space or America. But I know it's going to be America. I know all of you people from different places. From space, we really met in space. And Sally from Star City in Russia, you were my first roommate. And it's just amazing to see you all again up here. And it really hits me harder than I ever expected it would. Thanks for coming. And Andy, I look forward to helping you get adjusted. And I think you're in for a wonderful experience. One of the best of your whole life. Well, thanks, David. And Anatoly. It's a pleasure to see you. Thank you very much. Just as I've only been in here a few minutes, I can say I'm going to have an awful lot to learn despite all the training. But it's going to be a fascinating time. It's no doubt about that. So thank you. Thank you both. Anatoly will help you learn. It's a great pleasure to be back. I'm not only on through the space station near to see my old commander again, Anatoly. Great guy. And I'm sure Andy will learn a lot. It's very pleasant to see my colleagues on Mir. We've long waited this moment. We traveled for two days to this point. And finally have seen each other. And we are now seeing a number of happy and gleeful people. And I feel very fortunate to have been here, to be here among these people. Of course, this is the first time I am aboard Mir. This is a very impressive size. The scope of this is just unbelievable. I would like to express my thanks to everyone, to David, Anatoly and Pavel and Terry. I would like to like to greet all of the astronauts and cosmonauts who have arrived here. It is not possible for everyone on earth to come up here. But for those of us who are here, we are one family. We are all citizens of the world. And I greet you all.


It's New Year's! Remember New Year's Eve a year ago, 2019. Back then this year 2020 was nothing but future. New Year's Eve that's the day of the year that most of us think about the future more than any other day.  We set new goals and we promise to change things, change our behaviors. So what are we thinking and how do we think about the future? Well as you know one common theme is more or less. It might be more exercise, more money maybe, or more time with friends or for hobbies or less. Less junk food, less Facebook, less stress and work. These are priorities and priorities of course are important. If you exercise instead of smoking it will most probably affect your future. But this is also just extrapolating and the future is not so simple. It's not just an extension like more or less of what has been, of what we understand and know and everything we've been through. Our future is above all affected by what we don't know. It contains lots of experiences that none of us can imagine and even less expect. What if someone at that New Year's party where you were at last year said, look over there, can you see the coronavirus? Of course you can't because you don't know what to look for. One year ago most of us never heard of the coronavirus. I mean we didn't even know the word epidemiologist and even if you were to get a crash course during the fireworks, how likely do you think something like this would have sounded? Well this, I mean let me tell you that this got to be something. Let me give you a prediction. I predict they will cancel the Olympics. They will close every pub in all of London. They will close down India. That's the level we're at. You will be stuck inside a year in your pajamas. No, we don't think that way because we can't. When we think about the future we can worry and we can wish but only about what's in our imagination. What we cannot even imagine. That doesn't fit in our thoughts about the future. I mean you can do the opposite. You can do an exercise. You can be the future. Let's imagine if there was time travel. So let's bring a person here from the past. I'm Swedish so let's bring back a Viking. Now you are the future. Can you please explain a smartphone to this here, Viking? Remember he don't know what the internet is. He doesn't understand words like camera or GPS. This guy don't even know what a good old telephone is. That gap between you and the Viking. The gap we go to museums to experience. That gap is obviously the same between us and future museums. The future museums are constantly being filled. It's like a steady stream of incomprehensible news and crisis and innovations and smartphones, coronaviruses. And when we talk about these we talk about them and refer to them as good or bad. If I say innovation sounds good doesn't it? Crisis not so good. I want you to think of them not as good or bad but as decisive moments to remind you that they are never good or bad per definition. Decisive moments mean just one thing. It will never be the same again. It will never go back. When you know what a smartphone is or what a coronavirus is, you cannot unlearn it. And in that future museum this is the exciting place. This is where you stop. Your great grandchildren they will look at Steve Jobs when he introduced the first iPhone back in 2007 and Elon Musk with the first test line 2008. Then there is nothing. Nothing exciting for more than a decade. The economy is growing, stock market is up, all is good. The only thing fascinating is this Donald Trump character. Besides that nothing to think of and see. And then boom 2020. The year of the corona pandemic. Wow. This is an exciting part of the museum. This is a decisive moment. New normal. There are always the small, obvious, quite easy to spot opportunities in times like this. I like to refer to them as umbrellas. Like umbrellas when it's raining. It's quite easy to predict that this is an opportunity. The interest will grow. Now there is a growing market for things like designer face masks or these here are selfie masks. I mean obviously no future without our selfies. There is also a growing market for digital tool for communication meetings like this thing we're doing online right now and e-commerce. Everything that can help us in a world of physical distancing. So these are umbrellas on a rainy day. But then there are the not so easy to spot opportunities. A long-term change on the societal level. Change to the way we live our life. What if the transformation from office workers to out-of-office workers is just as profound as when we went from being farmers to industrial workers. Remote work. The opportunity might be something much bigger than just getting a smaller office. Maybe the real opportunity in this new world of remote workers is about a new society. Where people can move from big cities to smaller cities with a lower cost of living and higher quality of life. Where the most diverse and inclusive teams in history will emerge as a result. Now when companies can attract talent globally and where time as the main KPI for judging performance will be replaced by productivity output. Think of what that might mean to all the women. So many women still have to choose between professional life and family life. Now if managers instead have to learn to measure employees by output results rather than from how many hours they've spent together in the office or on the golf course. How well they know each other. Then maybe no one have to make that choice anymore. These are not predictions. These are opportunities and right now entrepreneurs, investors, leaders, people like you will spot opportunities like this and act. Maybe the only thing a small town needs to attract these remote workers is a great school. If they get some great teachers and then of course some super fast broadband maybe they can just sit back and enjoy how people and wealth is coming back again. Decisive moments are exciting moments. It is true when they say COVID-19 took us straight to 2030. One consequence of this pandemic is that much of what would have happened the coming 10 years now happened in just one. Obviously the old and already sick are suffering more than the young and healthy. Same in business. Weak companies the ones stuck in old business models having a hard time to transform and who would have died out now they do so in a faster pace. But just remember behind every bankrupted company every close storefront there is new opportunity. There is like this vacuum waiting to be filled. And another thing that leaped for this our use of digital tools for communication all the umbrellas I just told you about. I mean we were jumping online for all of this the coming 10 years anyway and now COVID-19 took us straight to 2030. But it is also true to describe the pandemic as life put on hold. Many have experienced 2020 as life in slow motion. All of a sudden we've had time you know that thing we never used to have time to think and reflect time to decide what's important and what's not. No matter what you think of this year at least it reminded us of one thing we cannot predict the future. No one can. I mean it's obvious now we were all wrong we didn't even have paper face masks for doctors. And this is an important reminder when we talk about the future like I always say the future is not to be predicted it is to be created. Success was never about predictions successful companies the ones we talk about all the time and Google, Amazon, Tesla they are not successful because their founders were good at guessing. The reason they are successful is because of a true understanding of the now. What problems need to be solved now and what opportunities are there now. So when you think about the future you should pay attention to this present moment. And I know this sounds like a no brainer one liner from a public speaker. But think again look around automobile companies still making hundreds of thousands of fossil fuel cars. The food industry gives unhealthy food that makes us sick. Retailers like in clothing totally unsustainable using resources and polluting all these unsustainable business models out there. This is ignoring today's problems and opportunities. It's quite obvious if you think of it like this. This here is GDP per capita. Thousands of years of progress or well rather thousands of years of no progress and then in just two three hundred years we got it all. Economic growth the fact that we today can create more human well-being in a week than that poor Viking could do in his whole lifetime. But again if we focus on the now today's problems let's add this graph. This is CO2 emissions and then it's quite clear that the way we created this fortune is not working. It's unsustainable. It's by burning fossil fuels and today we know that this is not a safe way and it's definitely not a way to to create more human well-being. Again only when we face the current situation with an attitude of acceptance then we can go on and be curious and think of action what needs to be done. That's how the future is born. It's not to be predicted it is to be created. You know what they say about this year depending on your understanding and then your action. You can come out of 2020 a hunk a monk or a drunk and now in just two weeks we're back watching the fireworks again some kind of physical distancing party and this time just like millions of Americans two months ago or so we will probably celebrate the end of something rather than looking forward. But you will celebrate the new year 2021 and the only thing we know about it is this 2021 will also bring change and you will adapt like you always do but for the future to be inspiring you need more than the ability to adapt. If you want to know how to cope with the speed of change and you want to create a great vision about the future make one that isn't about some thing you want. Make a vision about your relationship to uncertainty. You need to create a culture where you learn to enjoy uncertainty so when you wake up in the morning and and think of how uncertainty is the only certainty and and then just like a student you feel joy and excitement just because of that. Wow we are alive in extraordinary times of adventure and change and so much to learn still out there ahead of us so much to explore when that's your feeling then you should celebrate because the ones who learn to enjoy uncertainty those are the ones who will love our future. I wish you the best of luck and happy new year.