what is a probe in molecular biology?
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Molecular probe Please help improve the article with a good introductory style. (October 2009) (Learn how and when to remove this template message) A molecular probe is a group of atoms or molecules used in molecular biology or chemistry to study the properties of other molecules or structures.
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Molecular biology Molecular biology /məˈlɛkjʊlər/ concerns the molecular basis of biological activity between biomolecules in the various systems of a cell, including the interactions between DNA, RNA and proteins and their biosynthesis, as well as the regulation of these interactions.
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- Molecular Biology & DNA. Q: A: An intron is a nucleotide sequence found within the genes and is removed via RNA splicing when a mature RNA molecule is being generated; on the other hand, an exon is a nucleic acid sequence present in a mature messenger RNA molecule.
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- The probe is labeled on both strands (usually), so either strand can hybridize with a DNA strand on the filter. On the other hand, the labeled DNA can also re-anneal to its partner in solution -- the opposite labeled strand of DNA.
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Definitions &Translations Molecular biology /məˈlɛkjʊlər/ concerns the molecular basis of biological activity between the various systems of a cell, including the interactions between the different types of DNA, RNA and proteins and their biosynthesis, and studies how these interactions are regulated.
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probe Definition of probe for English Language Learners. : 1  a careful examination or investigation of something. : 2  a thin, long instrument that is used especially for examining parts of the body.
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Designing PCR primers and probes Design your PCR probes to conform to the following guidelines: 1  Location: Ideally, the probe should be in close proximity to the forward or reverse primer, but should not overlap with a primer-binding site on the same strand. 2  Probes can be designed to bind to either strand of the target.
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Hybridization probe DNA sequences or RNA transcripts that have moderate to high sequence similarity to the probe are then detected by visualizing the hybridized probe via autoradiography or other imaging techniques. Normally, either X-ray pictures are taken of the filter, or the filter is placed under UV light.ybridization probes used in DNA microarrays refer to DNA covalently attached to an inert surface, such as coated glass slides or gene chips, to which a mobile cDNA target is hybridized.
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- Microarrays are a collection of many short DNA strands, called “probes,” that are attached to a solid surface (e.g., a glass slide). The probes are selected for their specific, known DNA sequenceto which, only complementary pieces of DNA will bind (hybridize).
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A dual response near-infrared fluorescent probe for hydrogen polysulfides and superoxide anion detection in cells and in vivo A dual response near-infrared fluorescent probe for hydrogen polysulfides and superoxide anion detection in cells and in vivo. Received 28 May 2015, Revised 4 June 2015, Accepted 6 June 2015, Available online 10 June 2015.
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The Cu Chi Tunnels: Vietnam's Deep, Dark Past Whatever your take may be on the Vietnam War (or the American War, as the Vietnamese call it) and the causes for which it was fought, you have to credit the VC soldiers for their pluck and valor: At least 45,000 Vietnamese men and women died defending the Cu Chi tunnels.
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Nucleic acid secondary structure In molecular biology, two nucleotides on opposite complementary DNA strands that are connected via hydrogen bonds are called a base pair (often abbreviated bp). In the canonical Watson-Crick DNA base pairing, Adenine (A) forms a base pair with Thymine (T) and Guanine (G) forms a base pair with Cytosine (C).
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What is Molecular Biology? As a science that studies interactions between the molecular components that carry out the various biological processes in living cells, an important idea in molecular biology states that information flow in organisms follows a one-way street: Genes are transcribed into RNA, and RNA is translated into proteins.
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Genes related to development of refractory prostate cancer Sense, when applied in a molecular biology context, is a general concept used to compare the polarity of nucleic acid molecules, such as DNA or RNA, to other nucleic acid molecules.Depending on the context within molecular biology, sense may have slightly different meanings.ense, when applied in a molecular biology context, is a general concept used to compare the polarity of nucleic acid molecules, such as DNA or RNA, to other nucleic acid molecules.
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Fermentation Probe Sensors. The optek probe series of bioprocess analyzers were designed specifically to easily integrate into bioreactors and fermenters. These precise systems provide the user with a powerful tool to characterize cell growth in the laboratory and large scale cell culture and fermentation. The real-time biomass concentration is measured as a function of NIR absorption, which is less sensitive to air bubbles and coating than backscattering instruments.
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Can you describe the process of blood clotting? Blood Clotting Process: - Clotting factors are proteins in the blood that control bleeding. When a blood vessel is injured, the walls of the blood vessel contract to limit the flow of blood to the damaged area.
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Definitions &Translations Molecular biology. Molecular biology is the branch of biology that deals with the molecular basis of biological activity. This field overlaps with other areas of biology and chemistry, particularly genetics and biochemistry.
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Fluorescence in situ hybridization Probe size is important because longer probes hybridize less specifically than shorter probes, a short strand of DNA or RNA (often 10–25 nucleotides) which is complementary to a given target sequence, it can be used to identify or locate the target.. The overlap defines the resolution of detectable features.
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- ATP: The Energy Transfer Molecule. Adenosine 5'-triphosphate (ATP) is a multifunctional nucleotide, most important as the molecular currency of intracellular energy transfer. Like tiny rechargeable batteries, ATP molecules transport chemical energy within a biological cell.denosine 5'-triphosphate (ATP) is a multifunctional nucleotide, most important as the molecular currency of intracellular energy transfer. Like tiny rechargeable batteries, ATP molecules transport chemical energy within a biological cell.
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Hybridization probe DNA sequences or RNA transcripts that have moderate to high sequence similarity to the probe are then detected by visualizing the hybridized probe via autoradiography or other imaging techniques. Normally, either X-ray pictures are taken of the filter, or the filter is placed under UV light.
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Reporter gene Reporter gene. In molecular biology, a reporter gene (often simply reporter) is a gene that researchers attach to a regulatory sequence of another gene of interest in bacteria, cell culture, animals or plants.
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Penn Genomics Analysis Core DNA and RNA analysis on Agilent 2100 bioanalyzer. The Agilent 2100 Bioanalyzer (http://www.genomics.agilent.com), a microfluidics-based platform is suitable for sizing, quantification and quality control of DNA, RNA, proteins and cells. A fast automated run on the system for 30-40 minutes delivers high quality digital data.
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- In the future, geneticists may have PNA probe libraries available to test for multiple mutations on one or many genes. As the research team wrote in their letter to Nature, a PNA probe library . . . might form the basis for a universal screening strategy for any genetic disease with a known spectrum of mutations..
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- The method of choice for nucleic acid (DNA, RNA) quantification in all areas of molecular biology is real-time PCR or quantitative PCR (qPCR). The method is so-called because the amplification of DNA with a polymerase chain reaction (PCR) is monitored in real time (qPCR cyclers constantly scan qPCR plates).
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Appendix A: Units and Measures Cell biology deals with things which are relatively small. The units of measurement typically used are the micron at the light microscope level, and the nanometer at the electron microscope level. For molecular measurements, the norm is the Angstrom.
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Hybridization probe (December 2009). In molecular biology, X hybridization probe is a fragment of DNA or RNA of variable length (usually 100-1000 bases long) which is radioactively labeled.It can then be used in DNA or RNA samples to detect the presence of nucleotide sequences (the DNA target) that are complementary to the sequence in the probe.ybridization probes used in DNA microarrays refer to DNA covalently attached to an inert surface, such as coated glass slides or gene chips, to which a mobile cDNA target is hybridized.