plate reader dna rna concentration
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Measure DNA and RNA Quickly and Easily in SpectraMax Microplate Spectrophotometers Measure DNA and RNA Quickly and Easily in SpectraMax Microplate Spectrophotometers (Maxline Application Bulletin #7)(PDF) Summary: Accurate and reproducible A260 measurements can be made easily in SpectraMax Microplate Spectrophotometers with awareness of the optical properties of microplate materials and attention to technique.
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Both Viral Transcription and Replication Are Reduced when the Rabies Virus Nucleoprotein Is Not Phosphorylated Phosphorylation of rabies virus N affects both transcription and replication of the rabies virus minigenome. In our previous study (28) and the study described above, only CAT activities were assayed to measure viral transcription and replication.utation of the serine at position 389 of the rabies virus N to alanine also resulted in increased binding to leader RNA in comparison to that for wt N. In the present study, we demonstrated that N phosphorylation affects both viral transcription and replication.
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Amoxicillin Suspension In blood serum, amoxicillin is approximately 20% protein-bound. Orally administered doses of 250 mg and 500 mg amoxicillin capsules result in average peak blood levels 1 to 2 hours after administration in the range of 3.5 mcg/mL to 5 mcg/mL and 5.5 mcg/mL to 7.5 mcg/mL, respectively.
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- Despite the fact that the sensitivity of plate readers is rather low compared to fluorescent microscopy, measurements of [Ca 2+ ] i kinetics with plate readers would thus be favorable. Consequently, many laboratories now use plate readers and a large amount of HTS data on changes in [Ca 2+ ] i has become available.
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Agarose gel electrophoresis Agarose gel electrophoresis. Digital image of 3 plasmid restriction digests run on a 1% w/v agarose gel, 3 volt/cm, stained with ethidium bromide. The DNA size marker is a commercial 1 kbp ladder. The position of the wells and direction of DNA migration is noted.
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Agarose gel electrophoresis Digital image of 3 plasmid restriction digests run on a 1% w/v agarose gel, 3 volt/cm, stained with ethidium bromide. The DNA size marker is a commercial 1 kbp ladder. The position of the wells and direction of DNA migration is noted.
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Taq polymerase T. aquaticus is a bacterium that lives in hot springs and hydrothermal vents, and Taq polymerase was identified as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR. Therefore, it replaced the DNA polymerase from E. coli originally used in PCR. Taq's optimum temperature for activity is 75–80 °C, with a half-life of greater than 2 hours at 92.5 °C, 40 minutes at 95 °C and 9 minutes at 97.5 °C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72 °C.
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- The objective of this study was to select lipase-producing bacteria collected from different counties of the Amazon region. Of the 440 bacteria strains, 181 were selected for the lipase assay in qualitative tests at Petri dishes, being 75 (41%) lipase positive.The enzymatic index was determined during fifteen days at different temperatures (30°, 35°, 40°, and 45°C). The highest lipase activity was observed within 72 hours at 30°C.rom the 440 strains tested in the basic medium, 181 were chosen for testing lipase activity, since they presented a good preliminary development in that medium (up to 72 hours). From these, 75 strains (41%) showed enzymatic activities (Table 1). Table 1: Amount of lipolytic bacteria for each group analyzed.
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- Reverse transcription polymerase chain reaction Reverse transcription polymerase chain reaction (RT-PCR) is the most sensitive technique for detecting the presence of rotavirus and the PCR products can be used to determine the particular virus genotype and strain.
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- PON1 transfer from HDL to chylomicrons. HDL (0.9 mg of protein/mL) was incubated with chylomicrons (0.75 mg of triglycerides/mL) for 30 min at 37 °C, followed by chylomicrons (A) and HDL (B) re-separation from the mixture by ultracentrifugation for 20 min at 30,000 rpm.he concentration of serum chylomicrons in an average postprandial state, as used in our study, is about 1500 μg of triglycerides/mL, whereas the concentration of serum HDL is about 1800 μg of protein/mL.
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Isopropanol precipitation of DNA - duration and magnitude of cold storage up vote 2 down vote favorite. DNA prep protocols often include a final precipitation step with alcohol, often isopropanol, where the DNA must be kept in the alcohol, at a low temperature such as -20C or -70C, often overnight.
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- Home › Articles › Electrophoresis of DNA and RNA. The most popular alternative to glass powder elution for the complete purification of DNA from agarose is electroelution. Because agarose gels are run in a horizontal apparatus, the gel can be manipulated during a pause in the run.
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Test Center Clinical Significance. The detection of HSV-1 and HSV-2 DNA is based upon the real-time amplification, detection and differentiation of specific HSV-1 and HSV-2 genomic DNA sequences by PCR from total DNA extracted from the specimen. The quantitative range of this assay is 100-2,000,000 copies/mL.
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- Plate reader-based measurements of [Ca 2+ ] i have a rather low sensitivity compared to fluorescence microscopy (Fig. 2). Consequently, subtle effects may be overlooked in the plate reader. Additionally, the lack of single cell resolution may blur the interpretation of the obtained results.
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The Basics: RNA Isolation RNA quantitation is an important and necessary step prior to most RNA analysis methods. Here we discuss three common methods used to quantitate RNA and tips for optimizing each of these methods. The traditional method for assessing RNA concentration and purity is UV spectroscopy. The absorbance of a diluted RNA sample is measured at 260 and 280 nm. The nucleic acid concentration is calculated using the Beer-Lambert law, which predicts a linear change in absorbance with concentration (Figure 1).
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- The deoxyribonuclease (DNase) test detects the degradation of DNA by bacterial species that produce DNase. The DNase test may be performed on plate media and is available in some commercial tests, e.g., QuadFERM+.When DNase is produced by organisms in the QuadFERM+ test, an acidic endproduct is formed and the pH indicator changes from red (alkaline) to yellow (acid) e.g., Figure 1.Figure 1. Example of a Rapid DNase Test for Neisseria and Related Species.he DNase test may be performed on plate media and is available in some commercial tests, e.g., QuadFERM+. When DNase is produced by organisms in the QuadFERM+ test, an acidic endproduct is formed and the pH indicator changes from red (alkaline) to yellow (acid) e.g., Figure 1.
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Agarose gel electrophoresis The concentration of gel affects the resolution of DNA separation. For a standard agarose gel electrophoresis, a 0.8% gives good separation or resolution of large 5–10kb DNA fragments, while 2% gel gives good resolution for small 0.2–1kb fragments. 1% gels is often used for a standard electrophoresis.garose gel has lower resolving power than polyacrylamide gel for DNA but has a greater range of separation, and is therefore used for DNA fragments of usually 50-20,000 bp in size.
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QIAquick Gel Extraction Kit The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. DNA ranging from 70 bp to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl.
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RNA New Analytical Tool for Fluorescence Detection of Double-Stranded RNA. 1  Aug. 4, 2016 — Ribonucleic acid binding fluorescent probes have been powerful and important analytical tools for the study of RNA structures and functions. Researchers report a new RNA probe that binds to ...
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Biology 305 Laboratory We will use agarose gel electrophoresis to determine DNA fragment sizes, and to quantify DNA. This technique separates DNA molecules based on size. DNA has a net negative charge that is proportional to its size. When placed in a matrix of agarose and exposed to an electric field, DNA molecules will move towards the positive electrode.
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Comparison of the Real-Time PCR Method and the Gen-Probe Amplified Mycobacterium tuberculosis Direct Test for Detection of Mycobacterium tuberculosis in Pulmonary and Nonpulmonary Specimens However, real-time PCR seemed to be less susceptible to amplification inhibitors than AMTDII. During the last decade, a number of nucleic acid amplification (NAA) methods have been developed for rapid detection and identification of Mycobacterium tuberculosis complex (MTB) in clinical specimens (3, 5, 9, 10). These techniques are attractive due to the direct detection of few copies of genomic sequence of MTB in clinical specimens.
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- Different methods of RNA extraction were compared. Figure 2 demonstrates that Trizol and RNeasy spin columns render better results than the NP40 method. cDNA yields were not affected when glomerular tissue was stored for up to 56 days and at a temperature of -70°C in PBS or the RNase inhibiting compound RLT Figure 2.ethods. RNA integrity was compared between different methods for extraction of RNA, synthesis of cDNA, and storage of renal tissue. Hereby, RNA later, an RNA-preserving compound, was tested for implementation in a protocol for renal biopsies that combines routine histology and RNA expression studies.
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- LaFarge Lecture Series. The LaFarge Lecture Series, designed exclusively for the College Prep Program, provides students with the unique opportunity to attend engaging undergraduate seminars conducted by some of Georgetown University's most distinguished faculty and scholars.
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- Anti-ds DNA antibody [35I9 DNA] images. Immunocytochemistry - Anti-ds DNA antibody [35I9 DNA] (ab27156)This image is courtesy of an Abreview submitted by Dr Jan Postberg. ab27156 at 1/1000 staining Stylonychia lemnae cells (isolated macronucleus anlagen) by ICC. The cells were methanol fixed and incubated with the antibody for 1 hour.
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- Methods of DNA/RNA Purification from PAGE Gels. This technique accelerates the slow diffusion of DNA from a block of polyacrylamide by maximizing the surface area for contact between the gel and the elution buffer. The gel slice is crushed into a fine slurry, which is soaked in the elution buffer of choice.
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Gel electrophoresis The DNA size marker is a commercial 1 kbp ladder. The position of the wells and direction of DNA migration is noted. Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge.