which stain is used to measure sperm viability
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Review ArticleMeasurement of Concentration and Viability in Stallion Sperm Introduction. 1  Sperm concentration and sperm membrane intactness (SMI) or viability are two measures of sperm quality that provide important, but different, information about a stallion's reproductive capability. “ 2 Sperm viability” has been used for decades as a measure of sperm quality.
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- Forensic biology is used to determine what types of biological stains, such as blood or semen, are left at a crime scene and to link those stains to individuals through DNA analysis.1  DNA Analysis. 2  Serology. 3  Biology Screening. 4  Pathology/Forensic Anthropology.he Technical Working Group on Biological Evidence Preservation has released The Biological Evidence Preservation Handbook: Best Practices for Evidence Handlers. The Handbook addresses packaging and storage, tracking and chain of custody, and disposition of biological evidence.
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- The most commonly used stain for detecting DNA/RNA is ethidium bromide. Ethidium bromide is a DNA interchelator, inserting itself into the spaces between the base pairs of the double helix.
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Male Fertility Infertility Tests - Sperm Count Semen Analysis A semen analysis evaluates the amount and quality of a man’s semen and sperm to determine fertility. This test is sometimes called a sperm count. Semen analysis is used to assess the following: 1  Motility: movement of the sperm (swimming ability). 2  Morphology: percentage of sperm that have a normal shape.
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- The semen analysis also can be used to determine whether sperm are present in semen after a man has had a vasectomy, a surgical procedure that prevents sperm from being released within the ejaculate. This surgery is considered a permanent method of birth control (99.9%) when performed successfully. ^ Back to top.
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Microbiology Lab Practice test Dr F Endospore Stain. The endospore stain is a differential stain used to visualize bacterial endospores. Endospores are formed by a few genera of bacteria, such as Bacillus . By forming spores, bacteria can survive in hostile conditions. Spores are resistant to heat, dessication, chemicals, and radiation.
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- . Water is used to decolorize. the cells; as the endospores are resistant to staining, the endospores are equally resistant to de-staining and will retain the primary dye while the vegetative cells will lose the stain.The addition of a counterstain or secondary stain (safranin) is used to stain the decolorized vegetative cells.hen vegetative cells are subjected to environmental stresses such as nutrient deprivation they eventually die. However, some bacteria such as the Bacillus spp. and the Clostridium spp can circumvent the problems associated with environmental stress by forming. endospores. .
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- Cell structures made visible by staining with hematoxylin and eosin. The combination of hematoxylin and eosin is the most commonly used method to stain sections of tissues. Hematoxylin, a basic dye, binds to acidic components of a tissue, which are thus said to be basophilic..
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- That is why there does notneed to be a decolorizer in this stain: it is based on the binding of the malachite greenand the permeability of the spore vs. cell wall.. The steaming helps the malachitegreen to permeate the low-permeability spore wall.hat is why there does notneed to be a decolorizer in this stain: it is based on the binding of the malachite greenand the permeability of the spore vs. cell wall.
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- however not from the spore wall once the dye is locked in. That is why there does not need to be a decolorizer in this stain: it is based on the binding of the malachite green and the permeability of the spore vs. cell wall. . The steaming helps the malachite green to permeate the low-permeability spore wall. A variety of chemicals comprise the
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Toluidine Blue â A Histology Stain for Mast Cells (and Other Things!) Toluidine blue (TB) is a polychromatic dye which can absorb different colours depending on how it binds chemically with various tissue components. It first emerged in 1856, courtesy of a British chemist called William Henry Perkin.B stains tissues via the phenomenon of ‘ metachromasia ‘. Many dyes can show metachromasia, but the thiazine group dyes such as TB are especially good for this type of staining. This phenomenon refers to how a dye stains tissue components a different colour from the dye solution and the rest of the tissue.
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Types of Staining techniques used in Microbiology and their applications 4.38 / 5  ( 16 ) Negative stains are used when a specimen or a part of it, such as the capsule resists taking up the stain. India Ink preparation is recommended for use in the identification of Cryptococcus neoformans. Giemsa stain: Giemsa stain is a Romanowsky stain. It is widely used in Microbiology laboratory for the staining of: 1  Malaria and other blood parasites. 2  Chlamydia trachomatis inclusion bodies. 3  Borrelia species.  Yersinia 1  pestis. Histoplasma species.  Pneumocystis jiroveci cysts (formerly Pneumocystis carinii)
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- Intense staining was observed in almost all the spermatogenic cell types (from spermatogonia to elongated spermatids) in testis sections from CP-treated rats. The gene encodes a RNA binding protein that localizes to the nucleus of all spermatogenic cell types (4,20). Various parameters related to sperm quality--including motility, concentration, and spermatogenic cell density, among other measures of sperm health--were also assessed.
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Capsule Staining- Principle, Reagents, Procedure and Result 4.3/5  (253) The capsule differs from the slime layer that most bacterial cells produce in that it is a thick, detectable, discrete layer outside the cell wall. The capsule stain employs an acidic stain and a basic stain to detect capsule production. Principle of Capsule Staining Capsules stain very poorly with reagents used in simple staining and a capsule stain can be, depending on the method, a misnomer because the capsule may or may not be stained.
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- The primary stain applied is malachite green, which stains both vegetative cells and endospores. Heat is applied to help the primary stain penetrate the endospore. The cells are then decolorized with water, which removes the malachite green from the vegetative cell but not the endospore.ndospore Stain. Purpose: The endospore stain is a differential stain which selectively stains bacterial endospores. How it works: Bacterial endospores are metabolically inactive, highly resistant structures produced by some bacteria as a defensive strategy against unfavorable environmental conditions.
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- The gonorrhea test is used to: 1  Diagnose the cause of symptoms. 2  Screen sexually active people. 3  Document that a person has been sexually abused.n men, a quick method that may be used in a clinic or doctor's office is the gram stain, which allows the doctor to look at a sample from the urethra for the presence of the bacteria using a microscope. While this method can diagnose gonorrhea, it is not sufficient to rule out an infection in asymptomatic men.
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LAB 7: ENDOSPORE STAIN AND BACTERIAL MOTILITY Strong dyes and vigorous staining conditions such as heat are needed. Once stained, however, endospores are equally hard to decolorize. Since few bacterial genera produce endospores, the endospore stain is a good diagnostic test for species of Bacillus and Clostridium.
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- During the endospore stain protocol, a bacterial smear of an endospore-producing bacteria, such as Bacillus, is prepared. Then the dye malachite green is forced into the spore with heat from a water bath, in much the same way that fuchsin is forced through the waxy mycolic acid layer of Mycobacterium in the acid-fast stain. Virtual Microbiology.
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Histopathology To see the tissue under a microscope, the sections are stained with one or more pigments. The aim of staining is to reveal cellular components; counterstains are used to provide contrast. The most commonly used stain in histopathology is a combination of hematoxylin and eosin (often abbreviated H&E).he second method of histology processing is called frozen section processing. This is a highly technical scientific method performed by a trained histoscientist (a Medical Laboratory scientist specialist in Histopathology.
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Cell Viability and Proliferation Assays Assays that measure metabolic activity are suitable for analyzing proliferation, viability, and cytotoxicity. The reduction of tetrazolium salts such as MTT and XTT to colored formazan compounds or the bioreduction of resazurin only occurs in metabolically active cells.
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Reticulin stain A liver biopsy stained using the reticulin demonstrating the normal hepatic plate thickness and mild steatosis. In pathology, the reticulin stain, is a popular staining method in histology. It is used to visualize reticular fiber and used extensively in liver histo pathology. See also. H&E stain; Trichrome stain; References
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- The necessary temperature for maintaining sperm viability is far colder than a home freezer maintains. Specialized kits sold by only a few andrology clinics are designed to let you manage the collection process in the comfort of your own home. They can be purchased and shipped to you for about $350.
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Janus Green B Janus Green B has been used in staining technique to determine cell density, to detect the presence of telocytes in trachea and lungs and used as a dye to visualize bonghan ducts inside lymphatic vessels of rabbits. Biochem/physiol Actions Janus Green B belongs to the phenazine group of dyes. It is a dark green/dark brown/dark black powder. Janus Green B should be oxidized to become colored. Thus, this dye can be used to specifically stain mitochondria in living cells. It has been used to stain biomolecules, nucleic acids and chromosomes.
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- METHOD/PROCEDURE: 1  Place a drop of the CSF specimen on a clean glass slide. 2  Spread using a sterilized inoculating loop and allow to dry. 3  Heat fix the smear on the glass slide. 4  Flood the slide with methylene blue stain. 5  Allow for 5 minutes. 6  Allow the slide to dry completely. 7  Add a drop of immersion oil on the slide.
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Semen analysis The site of semen collection does not affect the results of a semen analysis. Measurement methods. Volume can be determined by measuring the weight of the sample container, knowing the mass of the empty container. Sperm count and morphology can be calculated by microscopy. Sperm count can also be estimated by kits that measure the amount of a sperm-associated protein, and are suitable for home use. [unreliable medical source?] Computer Assisted Semen Analysis (CASA) is a
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H&E stain H&E stain, HE stain or hematoxylin and eosin stain, is a popular staining method in histology. It is the most widely used stain in medical diagnosis; for example when a pathologist looks at a biopsy of a suspected cancer, the histological section is likely to be stained with H&E and termed H&E section, H+E section, or HE section.