why does dna dissolve in water
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- Split and merge into it. Answer by YungSan3. Confidence votes 6. yes DNA is soluble in water. the phosphate backbone is negative so it is polar. This polarity allows water, which is also a polar molecule, to dissolve the DNA.plit and merge into it. Answer by YungSan3. Confidence votes 6. yes DNA is soluble in water. the phosphate backbone is negative so it is polar. This polarity allows water, which is also a polar molecule, to dissolve the DNA.
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- Like in proteins, water is an integral part of nucleic acid structures. A shell of hydration, impermeable to cations, is located around DNA double helices [18]. It consists of about 18-19 water molecules per nucleotide in B-DNA and of about 13-14 in A-DNA.
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Why is DNA insoluble in ethanol and soluble in water? This makes DNA soluble in water. Alcohol on the other hand, is a non-polar molecule, meaning it has little to no charge. This type of chargeless molecule does not interact with and form hydrogen bonds with DNA and therefore cannot dissolve DNA.NA consists of proteins that are polar. Water is polar so it is soluble in water. Alcohol is also non-polar, so insoluble in ethanol...u owe me 10 pts for this one.
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Nucleic acid thermodynamics DNA denaturation, also called DNA melting, is the process by which double-stranded deoxyribonucleic acid unwinds and separates into single-stranded strands through the breaking of hydrophobic stacking attractions between the bases.See Hydrophobic effect.he process of DNA melting is also used in molecular biology techniques, notably in the polymerase chain reaction. Although the temperature of DNA melting is not diagnostic in the technique, methods for estimating Tm are important for determining the appropriate temperatures to use in a protocol.
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- This is because when a solute is dissolved in water, its molecules (or. Lab 3: Diffusion and Osmosis (Revised Winter 2011) Lab 3 - Biol 100 - Page 2 of 7 ions) fill the space previously occupied by water molecules, thus reducing the concentration of water.
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- In this case, go back to the first step and add less water. You need an opaque cell ‘soup’ for good yields. The amount of DNA you will get will depend on the ratio of DNA to cell volume rather than the number of chromosomes in your material. Plant seeds (such as peas) contain a high proportion of DNA. 3 The salt added helps the DNA to precipitate (as it clumps together) when it meets the ethanol phase. 4 It is important to allow time for each step to complete. The detergent must sit for at least 5 minutes to disrupt the cell membranes and nuclear membranes.
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Why do you add dish soap to extract DNA out of a liver cel?? Since DNA is much more soluble in alcohol than in water. The DNA will migrate to the alcohol layer and condense, where you can then wind it around a stick, without having to deal with the the remains of the cells.
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- DNA helicase causes the hydrogen bonds to melt, which opens up the DNA at the site where DNA helicase attaches to the DNA double helix. This action will continue as DNA helicase moves down the length of the DNA strands, just like a zipper gives more access the further down it's pulled. As DNA helicase continues to move, it creates a replication fork, which is the open area of DNA where replication or transcription can take place.
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- The dsDNA molecule is quite robust and stable in the correct conditions. For example, careful purification of DNA only takes about one hour, and we keep the DNA disolved in Tris buffer (pH 7.5-8.5) with some EDTA to trap Mg++ ions. (DNAase enzymes that cut up DNA need Mg++ for activity, so EDTA keeps them inactive).
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- Several different forms of sodium are used in DNA extraction. Sodium dodecyl sulfate, or SDS. is a sodium-containing detergent. It has the chemical formula of C12H25NaO4S, where the Na stands for sodium. Detergents are used to break down cell walls and membranes.odium hydroxide is a base. A solution of sodium hydroxide makes the solution very basic or alkaline. Sodium hydroxide can act by loosening the rigid structure of a cell wall or membrane, thereby releasing the DNA. Sodium hydroxide is most often used in plasmid DNA extraction.
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DNA Extraction? Alcohol? Best Answer: You add alcohol in order to concentrate the DNA and in order to get rid of the salts and other chemicals used in the extraction process.As others said above, DNA precipitates in alcohol.est Answer: You add alcohol in order to concentrate the DNA and in order to get rid of the salts and other chemicals used in the extraction process.
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DNA Precipitation: Ethanol vs. Isopropanol DNA falls out of solution in 35% isopropanol and 0.5M salt. Using ethanol, the final concentration needs to be around 75% with 0.5M salt. So for the typical precipitation protocol, isopropanol is added from between 0.7-1 volumes of sample and ethanol is added at 2-2.5 volumes of sample.
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The sciences behind the rise of biotechnology DNA extraction is the process by which a cell is broken open to expose and extract its DNA. This is done by breaking down and emulsifying the fat and proteins that make up the cell's membrane through the addition of both salt and detergent solutions. Then the DNA is separated by by adding alcohol and centrifuging the resulting solution.
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Why are hydrogen bonds so important for living things? Hydrogen bonds hold the two strands of DNA together and is a component of the Base pairing in nucleic acids. Hydrogen bonding allows water to remain liquid at room temperature when it would otherwise be a gas. It allows a number of nitrogen and oxygen containing compounds (such as glucose and urea) to pass easily through the body. Hydrogen bonds hold the two strands of DNA together and is a component of the Base pairing in nucleic acids.
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- The word detergent is used instead of soap in a DNA extraction buffer. Detergent is used to create a hydrophobic environment that favors the precipitation of proteins. Protein…s are one of two major contaminants in DNA extraction (the other major contaminant being RNA).
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Be Part of Ask A Biologist An electric current is used to move the DNA molecules across an agarose gel, which is a polysaccharide matrix that functions as a sort of sieve. The matrix helps catch the molecules as they are transported by the electric current.
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Extraction of DNA from White Onion 1 When sodium is added to DNA it forms a protective shell around it. 2  On the other hand, protein molecules precipitate from solution in the presence of salt. 3  DNA is insoluble in ethanol (ethyl alcohol). 4  As ethanol is added to a solution containing DNA, the DNA will come out of solution and stick to whatever is around.rocedure: 1  Place 100 ml of homogenizing solution in a beaker heat the solution in a water bath until it reaches 60 C. 2  Mince the onion and add to the solution when it has reached 60 C. Stir and let sit for 15 minutes.
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Polymerase chain reaction (PCR) Before PCR can occur, the two strands in the DNA double helix need to be separated. This is called denaturation. It is done by raising the temperature of the DNA solution. This causes the hydrogen bonds between the complementary DNA chains to break, and the two strands separate.efore PCR can occur, the two strands in the DNA double helix need to be separated. This is called denaturation. It is done by raising the temperature of the DNA solution. This causes the hydrogen bonds between the complementary DNA chains to break, and the two strands separate.
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How can we destroy DNA? DNA is a nucleic acid consisting of a nitrogenous heterocyclic base, a pentose sugar, and a phosphate group. DNA can be destroyed by the same substances that can destroy other human tissue-intense heat, strong ionizing radiation, a strong base such as sodium hydroxide, or a strong acid such as hydrofluoric acid.NA is a nucleic acid consisting of a nitrogenous heterocyclic base, a pentose sugar, and a phosphate group. DNA can be destroyed by the same substances that can destroy other human tissue-intense heat, strong ionizing radiation, a strong base such as sodium hydroxide, or a strong acid such as hydrofluoric acid.
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DNA Precipitation: Ethanol vs. Isopropanol DNA falls out of solution in 35% isopropanol and 0.5 M salt. Using ethanol, the final concentration needs to be around 75% with 0.5 M salt. So for the typical precipitation protocol, isopropanol is added from between 0.7–1 volumes of sample and ethanol is added at 2-2.5 volumes of sample.ut because salts are generally less soluble in isopropanol than in ethanol, they have more of a tendancy to co-precipitate with the DNA. So to lessen the chances of salt precipitation, isopropanol precipitations are carried our at room temperature with minimal incubation times.
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Why Does Water Dissolve Sugar? 1 For a liquid to dissolve a solid, the molecules of the liquid and solid must attract one another. 2  The bond between the oxygen and hydrogen atoms (O–H bond) in sugar (sucrose) gives the oxygen a slight negative charge and the hydrogen a slight positive charge.3  Sucrose is a polar molecule. The polar water molecules attract the negative and positive areas on the polar sucrose molecules which makes sucrose dissolve in water. 2  A nonpolar substance like mineral oil does not dissolve a polar substance like sucrose.
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- Making the world better, one answer at a time. No, DNA is not soluble in ethanol. When the 2 are mixed, DNA can be spooled out by stirring the solution with a glass rod.lucose is sparingly soluble in ethanol. This is because the organic molecules of the ethanol are too large to separate the glucose, unlike water where the water molecules a … re easily able to dissolve the crystalline structure to react with the-OH groups.
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Why Is DNA Soluble in Water? Polar molecules have atoms that are able to form hydrogen bonds, typically hydroxyl (-OH) or carbonyl (C=O) groups. The DNA backbone consists of alternating ribose (sugar) and phosphate molecules. Phosphate is negatively charged, which is why DNA macromolecules are predominately negative.
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- The original strand of DNA is unzipped by an enzyme that breaks the  hydrogen bonds between the bases. This process continues until the  entire strand of DNA has been unzipp … ed and copied.1 person found this useful.he original strand of DNA is unzipped by an enzyme that breaks the  hydrogen bonds between the bases. This process continues until the  entire strand of DNA has been unzipp … ed and copied. 1 person found this useful.
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- DNA is a long, stringy molecule. The salt that you added in step one helps it stick together. So what you see are clumps of tangled DNA molecules! DNA normally stays dissolved in water, but when salty DNA comes in contact with alcohol it becomes undissolved.