what is the basic principle of cyanmethemoglobin determination of hemoglobin?
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- Methods for hemoglobinometry can be grouped into 4 main classes depending on the basic technique employed with variants within each class: 1. Colorimetric Methods. 2. Gasometric Methods. 3. Specific Gravity Methods. 4. Chemical Methods. The method of choice for hemoglobin determination is the cyanmethemoglobin method (This is a type of colorimetric method). The principle of this method is that when blood is mixed with a solution containing potassium ferricyanide and potassium cyanide, the potassium ferricyanide oxidizes iron to form methemoglobin.
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An Overview of Hemoglobin HEMATOCRIT (HCT)- The hematocrit refers to the 'percentage' of one's red blood cells. HEMOGLOBIN (HGB) - Hemoglobin is a protein that is carried by red cells. It picks up oxygen in the lungs and delivers it to the peripheral tissues to maintain the viability of cells.
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hemoglobin test Description. Hemoglobin is made of heme, an iron compound, and globin, a protein. The iron gives blood its red color. Hemoglobin tests make use of this red color. A chemical is added to a sample of blood to make the red blood cells burst.
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Hemoglobin Concentration (Hb) Description. Hemoglobin is the main component of red blood cells and serves as the transporter for oxygen and carbon dioxide in the blood. Hemoglobin concentration (Hb) is usually reported as grams of hemoglobin per deciliter of blood (g/dL).Hb is commonly measured by the cyanhemoglobin (hemoglobin cyanide) method.emoglobin concentration (Hb) is usually reported as grams of hemoglobin per deciliter of blood (g/dL). Hb is commonly measured by the cyanhemoglobin (hemoglobin cyanide) method.
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4. Transport of Blood Gases and Metabolism of Hemoglobin Hemoglobin (Hb), the pigment that gives blood its red colour, provides the transport of blood gases. It carries oxygen from lungs to the rest of the body and a portion of carbon dioxide in the opposite direction. Hb transports 98 % of the total oxygen present in the blood and 23 % of CO2. Hemoglobin also forms an important blood buffer system, which removes the excess of H+ (particularly in peripheral tissues) by binding them to the His- residues of its molecule.
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Optical Absorption of Hemoglobin Hematocrit determines the fraction of the blood that is red blood cells. The red blood cells are primarily composed of hemoglobin (95% of the dry mass). 2 of functional hemoglobin combines with 1.34ml O 2, the O 2 capacity of normal blood is (150g Hb/liter)(1.34ml O2 g Hb) = 200ml O2/liter.
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- Hemoglobin Concentration. Whole blood is diluted in cyanmethemoglobin reagent.1,2 This reagent hemolyzes the erythrocytes which releases hemoglobin into the solution. The ferrous ions (Fe2+) of the hemoglobin molecules are oxidized by potassium ferricyanide to ferric ions (Fe3+). This oxidation results in the formation of methemoglobin.
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mean corpuscular hemoglobin Hb, Hbg, Hgb. The iron-containing pigment of red blood cells (RBCs) that carries oxygen from the lungs to the tissues. The amount of hemoglobin in the blood averages 12 to 16 g/100 ml in women, 14 to 18 g/100 ml in men, and somewhat less in children.hus, each hemoglobin molecule can bind one molecule of oxygen. The binding of oxygen by one monomer increases the affinity for oxygen of the others in the tetramer. This makes hemoglobin a more efficient transport protein than a monomeric protein such as myoglobin.
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- SaO 2-oxygen saturation, calculated. pH, pCO. 2, and pO. 2 are the primary measurements of the blood gas machine. Other terms that are used when discussing blood gas analysis. Base deficit or excess--the difference between a patients measured serum bicarbonate level.
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Medical Definition of Hemoglobin Hemoglobin: The oxygen-carrying pigment and predominant protein in the red blood cells. Hemoglobin forms an unstable, reversible bond with oxygen.In its oxygenated state it is called oxyhemoglobin and is bright red. In the reduced state it is called deoxyhemoglobin and is purple-blue. Each hemoglobin molecule is made up of four heme groups surrounding a globin group.Heme contains iron and gives a red color to the molecule. Globin consists of two linked pairs of polypeptide chains.n the reduced state it is called deoxyhemoglobin and is purple-blue. Each hemoglobin molecule is made up of four heme groups surrounding a globin group. Heme contains iron and gives a red color to the molecule. Globin consists of two linked pairs of polypeptide chains.
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- Synthesis of Heme. Heme is the prosthetic group of hemoglobin, myoglobin, and the cytochromes. The heme of cytochrome c is shown at right. (For the slightly different structure of heme a, see the notes on electron transfer.) Heme is an asymmetric molecule. (Note the positions of the methyl side chains around the ring system.) The heme ring system is synthesized from glycine and succinyl-CoA.
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- Additional Info. The first step in a BOD determination is taking a water sample from the test area and diluting it with a known volume of distilled water. The solution is then thoroughly shaken to insure oxygen saturation.dditional Info. The first step in a BOD determination is taking a water sample from the test area and diluting it with a known volume of distilled water. The solution is then thoroughly shaken to insure oxygen saturation.
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- Hemoglobin normal values: Hemoglobin is the oxygen-carrying pigment in the blood, the predominant protein in the red blood cells.emoglobin normal values: Hemoglobin is the oxygen-carrying pigment in the blood, the predominant protein in the red blood cells.
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Overview Definition. A hemoglobin test measures the amount of hemoglobin in your blood. Hemoglobin is a protein in your red blood cells that carries oxygen to your body's organs and tissues and transports carbon dioxide from your organs and tissues back to your lungs. If a hemoglobin test reveals that your hemoglobin level is lower than normal, it means you have a low red blood cell count (anemia). Anemia can have many different causes, including vitamin deficiencies, bleeding and chronic diseases.
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- Charge maintained by chloride entering RBC (chloride shift) Hydrogen ions combine with haemoglobin to form haemoglobonic acid. Hydrogencarbonate ions Releasing oxygen The partial pressure of oxygen (also called oxygen tension) of respiring tissues is lower than the lungs.
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- THE COLORIMETRIC DETERMINATION OF. HEMOGLOBIN.*. BY WALTER W. PALMER. (From the Hospital of The Rockefeller Institute for Medical Research.) (Received for publication, November 30, 1917.) The method to be described for the determination of hemoglobin. depends upon the comparison, in a calorimeter, of carbon mon-. oxide hemoglobin solutions, one of which has a known hemo-. globin content.
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Hemoglobin Concentration (Hb) Anemia is considered to be present if the Hb or the hematocrit (Hct) is below the lower limit of 2 standard deviations (-2SD) or the 95% confidence interval for the normal population. This definition of anemia results in 2.5% of normal individuals being classified as anemic.emoglobin concentration (Hb) is usually reported as grams of hemoglobin per deciliter of blood (g/dL). Hb is commonly measured by the cyanhemoglobin (hemoglobin cyanide) method.
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What is bromothymol blue? Bromthymol Blue indicates Carbon dioxide is present. It is also a general acid-base indicator. When it turns green it is at the endpoint of the titration. When it turns yellow … , it is overshot and thus acidic.
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Hemoglobin Level Test Hemoglobin is a protein that is contained within red blood cells (RBCs). Hemoglobin is the part of the red blood cell that carries oxygen to, and carbon dioxide away from, the cells in the body. Without the right amount of hemoglobin in the red blood cells, the cells of the body will not be able to receive enough oxygen.
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media tests indicator bromythol blue in OF medium. 2 tubes used, one sealed, the other open.turns yellow in the presence of acids,indicating catabolism of carbohydrates.dark blue/black color is produced in the presence of alkaline solution due to the use of pepton and npt carbohydrates because ammonia is produced.
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- Other method takes advantage of the varying conductivities of blood at. different concentrations of blood. In Hemoglobincyanide method hemoglobin is chemically converted to form a cyanmethemoglobin. which is having maximum absorption around 540nm wavelength. The hemoglobin concentration is determined.
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- HCl, then indicating the number of moles of sorbate ion using the C 6 H 7 O 2 −: HCl mole ratio, and then subsequently calculating the correct [KC 6 H 7 O 2]. The first point in part (c) was earned for selecting thymol blue as the indicator to use in the titration. The second point was earned for indicating that the color change
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- Background for the Kjeldahl determination of Organic Nitrogen. The Kjeldahl method is an analytical method for the determination of nitrogen in the trinegative state in certain organic compounds. The method was developed in 1883 by Johan Kjeldahl, a Danish chemist, and is used extensively in the determination of protein in foods, since protein is a macromolecule made up of nitrogen containing amino acids linked together.